Recombinant human sHer2 produced by HEK 293 and sHer2 isolated from the supernatant of the primary tumor cell culture from breast cancer patients was used in the experiments. The oligomeric status was estimated using the method of gel-filtration chromatography and electrophoresis. The stability of Her2 was determined by the change in the protein secondary structure under variation of temperature and pH values by means of CD spectroscopy. The inhibitory activity of sHer2 was analyzed in tests with Her2-expressing cell cultures. The content of sHer2 in the supernatant of primary tumor cell cultures from breast cancer patients was determined using the sandwich ELISA. Amplification of the HER2 gene in the primary tumor cells was verified employing the FISH. ...
We investigated the antiproliferative effects of extracellular nicotinamide adenine dinucleotide against human malignant CaCo-2 (colon carcinoma), Hep
Zhang M, Lykke-Andersen S, Zhu B, et al. Characterising-regulatory variation in the transcriptome of histologically normal and tumour-derived pancreatic tissues. Gut. 2018;67(3):521-533. doi:10.1136/gutjnl-2016-313146. ...
Zhang M, Lykke-Andersen S, Zhu B, et al. Characterising-regulatory variation in the transcriptome of histologically normal and tumour-derived pancreatic tissues. Gut. 2018;67(3):521-533. doi:10.1136/gutjnl-2016-313146. ...
TY - JOUR. T1 - Characterization of a newly established human pancreatic carcinoma cell line, UK Pan-1. AU - Fralix, Kimberly D.. AU - Ahmed, Mansoor M.. AU - Mattingly, Cynthia. AU - Swiderski, Carol. AU - McGrath, Patrick C.. AU - Venkatasubbarao, Kolaparthi. AU - Kamada, Nanao. AU - Mohiuddin, Mohammed. AU - Strodel, William E.. AU - Freeman, James W.. PY - 2000/5/1. Y1 - 2000/5/1. N2 - BACKGROUND. A highly tumorigenic cell line designated as UK Pan-1 was established in a surgically removed human pancreatic adenocarcinoma and characterized as having many of the genotypic and phenotypic alterations commonly found in pancreatic tumors. METHODS. The cell line was characterized by its morphology, growth rate in monolayer culture and soft agar, tumorigenicity in nude mice, and chromosomal analysis. Furthermore, the status of p53, Ki-ras mutation and transforming growth factor (TGF)-β receptor expression were determined. The characteristics of UK Pan-1 were compared with those of other commonly ...
Cytokine production by the human bladder carcinoma cell line T24 in the presence of bacillus Calmette-Guerin (BCG).: The study was initiated as an in vitro appr
Substantial multiplication in vitro of cloned cells from a human embryonal carcinoma cell line, Tera 2, has been obtained in a basal medium (DMEM/Hams F12,50:50, v/v) supplemented with 10 micrograms low density lipoprotein/ml, 100 micrograms high density lipoprotein/ml, 100 ng multiplication stimulating activity/ml, 100 ng insulin/ml and 1 microgram transferrin/ml. The growth rate appears to be similar to that obtained in 10% serum. Furthermore, studies on the expression of cell surface receptors revealed that cloned Tera 2 cells express high-affinity receptors for IGF-II but not for insulin. The cells also express receptors for Epidermal Growth Factor (EGF) even though the addition of EGF does not stimulate their proliferation in serum-free medium. These results suggest that the expression of specific growth factor receptors is not an absolute determinant of hormone responsiveness. ...
Apoptosis is a form of cell death in which the cell actively participates. Apoptosis was induced in two human leukaemic cell lines, U937 and HL-60, by incubation with a diverse array of chemical agents. Cell death was assessed by gel electrophoresis, light microscopy and flow cytometry. It was demonstrated that apoptosis involved the formation of large kilobase pair DNA fragments (20-50, 145-245 and 580 kilobase pairs) prior to, or accompanying, internucleosomal cleavage. Degradation of DNA to large kilobase pair sizes also occurred in some forms of necrosis. These fragments were similar, but not identical, to those generated during apoptosis. The identity of the endonuclease(s) responsible for DNA cleavage to large kilobase pair fragments is as yet unknown. One suggestion is that topoisomerase II might be involved. Using an HL-60 subclone with reduced topoisomerase II expression, it was shown that topoisomerase II was not necessary for the formation of large kilobase pair DNA fragments and ...
TY - JOUR. T1 - Expression of the matrix metalloproteinase promatrilysin in coculture of prostate carcinoma cell lines. AU - Stratton, M. S.. AU - Sirvent, H.. AU - Udayakumar, T. S.. AU - Nagle, R. B.. AU - Bowden, G. T.. PY - 2001/8/22. Y1 - 2001/8/22. N2 - BACKGROUND. Matrix metalloproteinases (MMPs) are involved in tumor progression. Matrilysin (MMP-7) has been shown to be upregulated in prostatic carcinomas and can increase the invasive capacity of DU-145 cells. Because of the heterogenous nature of prostatic tumors, we examined promatrilysin expression in cocultures containing two different prostatic carcinoma cell lines, DU-145 and LNCaP. METHODS. Using enzyme linked immunosorbent assay (ELISA) analyses, promatrilysin expression was measured in DU-145/LNCaP cocultures and conditioned media cross-cultures. The effects of blocking IL-6 on promatrilysin expression were examined by pretreating conditioned media with IL-6 neutralizing antibody. RESULTS. A significant induction of promatrilysin ...
BioAssay record AID 103718 submitted by ChEMBL: In vitro cytotoxicity against human breast carcinoma cell line MCF-7 at 8.1*10e-5M.
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Polyamines (PAs) are involved in regulation of cell growth and cellular survival by interacting with processes like translation, transcription or ion transport. It is described that polyamines can induce apoptosis in mesenchymal cell lines. The aim of our study was to analyze whether the physiological PAs (putrescine, spermidine or spermine) or the PA-derivate deoxyspergualin (DSG), a novel immunosuppressant, induce apoptosis in immunocompetent cells. Furthermore, we wanted to investigate which molecular mechanisms are involved in the execution of the cell death program. By means of flow cytometric analysis we found an induction of apoptosis by spermine (Spm) and DSG in quiescent and activated PBMCs, PHA generated lymphoblasts, and various tumor cell lines (Jurkat, SKW-3, U937). Moreover, DSG and Spm triggered apoptosis in human Fas-deficient cells and in cell lines MV4.11. and RS4.11., which are described to be resistant to apoptosis induction by many conventional chemotherapeutic agents. ...
BioAssay record AID 104079 submitted by ChEMBL: In vitro cytotoxicity against human mammary carcinoma cells 180 fold resistant to adriamycin MCF7-R.
1 Growth of Human Lung Tumor Cells in Culture Reen Wu Center for Comparative Respiratory Biology and Medicine, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California,
Tumour cells release membrane micro(nano)fragments called tumour-derived microvesicles (TMV) that are believed to play an important role in cancer progression. TMV suppress/modify antitumour response of the host, but there is also some evidence for their direct interaction with cancer cells. In cancer patients TMV are
3020 A variety of human carcinoma cells express high levels of interleukin-13 receptorα2 chain, a primary binding subunit of IL-13R complex in vitro and in vivo. These receptors could be targeted by a chimeric fusion protein consisting of Interleukin-13 and a truncated form of Pseudomonas exotoxin (IL-13PE38). Microarray analysis of adrenomedullin (AM) transfected human prostate tumor PC-3 cells showed that human and rat AM up-regulated the IL-13Rα2 chain gene. We now demonstrate that IL-13Rα2 chain is also up regulated in two human breast tumor cell lines (MCF-7 and MDA-MB-231) and one prostate carcinoma (PC-3) cell line after treatment with AM. RT-PCR results confirmed that mRNAs for IL-13Rα2 were enhanced 2.5 to 4 fold in 24 and 48 hr AM treated cells, respectively compared to control cells. Indirect immunofluorescence assay (IFA) revealed that protein level of IL-13Rα2 were also increased in AM treated breast and prostate carcinoma cells. To understand the mechanism of up-regulation, ...
Choong et al. used a database of expressed proteins for a hepatocellular carcinoma-derived cell line (HCC-M), separated by two-dimensional gel electrophoresis, that they had developed previously. The separated proteins were fingerprinted by using peptide mass spectrometric techniques. From these data they could identify novel proteins that were not present in the SWISS-PROT or National Center for Biotechnology Infromation (NCBI) databases. One such protein was then taken for further analysis and was subjected to in-gel trypsin digestion and the products were sequenced using mass spectrometry. The sequences were then used to search an expressed sequence tag (EST) database and 40 DNA sequences obtained were assembled into a putative open reading frame. On the basis of this consensus DNA sequence, the authors named the protein HCC-1, and confirmed and extended the sequence using RACE (rapid amplification of cDNA ends).. A battery of bioinformatics tools was used to analyze the predicted protein, ...
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Buy our SK N SH (human neuroblastoma cell line) whole cell lysate. ab3956 has been validated in western blot. Abcam now offers a 12-month guarantee.
By analysing a human lung tumour cell line subtraction cDNA library, we have identified and characterized a novel member of the human S100 gene family that we designated S100A14. The full-length cDNA is 1067 bp and encodes a putative protein of 104 amino acids. The predicted protein contains the S100-specific EF-hand calcium-binding domain. The gene is ubiquitously expressed in normal human tissues of epithelial origin. S100A14 transcript was found to be down-regulated in many immortalized and tumour cell lines from diverse tissues. In contrast to the tumour cell lines, S100A14 shows up-regulation at the mRNA and protein level in many human primary tumours, including lung and breast carcinomas. To elucidate mechanisms whereby S100A14 expression is enhanced in lung and breast tumours, we studied the effects of epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) on its expression. Both are ligands of ERBB receptor and induced S100A14 expression in the immortalized ...
Cellular Biology:, Neoplasm:, Serology: Antigen, Tissue Culture:, Types of Tumors:, Transplantable Tumors: YCAB, Genes: H-2 - Histocompatibility-2, Strains: BALB/C. ...
IL-13Rα2 chain has been shown to play a unique role in tumor biology. It is overexpressed in a variety of primary tumor cell cultures and tumor cell lines (13, 15-18, 25, 34), whereas normal cells including lymphoid cells, endothelial cells, and astrocytes (13, 15, 17, 26) do not express or express low levels of this cytokine receptor chain. Recent studies have demonstrated that overexpression of IL-13Rα2 chain in certain breast and pancreatic cancer cell lines resulted into loss of tumorigenicity, whereas unmodified control tumor cells formed enlarging tumor nodules when injected in immunodeficient mice (46). In another study, Terabe et al. (47) have demonstrated that the soluble form of IL-13Rα2 chain (5) can modulate immune environment and shift Th2 phenotype to a dominant Th1 phenotype. This shift resulted into resistance of 15-12RM cell-derived tumor recurrence. Thus, further investigation of the role of IL-13Rα2 chain in tumor immunology and targeting has become extremely ...
Work in this thesis was primarily involved with the characterisation of four receptors, the turkey and bovine P2Y1, and the human P2Y2 and P2Y4 receptors, transfected into the human astrocytoma cell line, 1321N1. It also describes the preliminary characterisation of two vascular smooth muscle cell types, and the generation of P2Y1-GST fusion proteins for the production of antibodies.;1321N1 cells were found to release nucleotides in response to sheer stress, but this was overcome by adaptation of the stimulation method. 1321N1 cells expressing the four receptors were assayed for PLC activity, and the rank order of agonist potency for each receptor was generally consistent with reports in the literature. Nucleotide interconversion at the surface of 1321N1 cells was also examined indirectly using the ecto-ATPase inhibitor ARL 67156 and hexokinase. Although there was no significant effect of nucleotide breakdown on the agonist concentration-responses, it was found that upstream conversion of ...
Many cancer patients develop tumor-reactive immune responses against antigens that are either expressed on the surface of tumor cells or released from them into the peripheral circulation. In this study, tumor-reactive immunoglobulins, present in the sera of ovarian cancer patients, were used to identify commonly recognized tumor-associated antigens on ovarian tumor cells. Western immunoblot analysis of cellular proteins, obtained from UL-1 ovarian tumor cell line, demonstrated several commonly recognized immunoreactive proteins. Two of these proteins (Mr 32,000 and 71,000) were selected for further investigation. Cellular proteins isolated from normal human ovarian epithelia, in a similar fashion, failed to exhibit corresponding immunoreactivity to these proteins. As an additional control, sera from normal (nontumor-bearing) individuals failed to identify these proteins on Western immunoblots. Furthermore, the absorption of the ovarian cancer patients sera with normal ovarian epithelial tissue ...
Cell culture. Six gastric cancer cell lines (KE-39, KE-97, HuG1-N, HuG1-PI, ECC-10, and ECC-12) were incubated in RPMI 1640 with 10% FCS (Life Technologies/Invitrogen Corp.) at 37°C. Other tumor cell lines were maintained in DMEM with 10% FCS (Life Technologies/Invitrogen Corp.) at 37°C. FK228 (a gift from Fujisawa Corporation) was used at concentrations of 1.0 to 2.5 ng/mL.. Plasmids and transfection. pCAGF1-IG (empty vector), pCAGF1-IG-Brm, pCAGF1-IG-Brm-KR, pCAGF1-IG-BRG1, and pCAGF1-IG-BRG1-KR were prepared as described previously ( 26). All transfections were done using Lipofectamine Plus (Invitrogen Corp.).. Western blotting. An anti-Brm antibody (Transgenic, Inc.), anti-BRG1 antibody (H-88; Santa Cruz Biotechnology), and anti-BAF47/Ini1 antibody (BD Transduction Laboratories) were used, and immunostaining was done as described previously ( 24).. Immunohistochemistry. Cells were fixed with 4% paraformaldehyde at 37°C for 1 h. After treatments with 0.2% Triton-X100 for 20 min at room ...
A stable LifeAct-TagGFP2 expressing human fibrosarcoma cell line Long-term actin visualization - 25 passages guaranteed Characteristics proven to be identica...
Aberrant growth factor production is a prevalent mechanism in tumourigenesis. If T-cells responded positively to a cancer-derived cytokine, this might result in selective enhancement of function within the tumour microenvironment. Here, we have chosen colony-stimulating factor-1 (CSF-1) as a candidate to test this concept. CSF-1 is greatly overproduced in many cancers but has no direct effects upon T-lymphocytes, which do not express the c-fms-encoded CSF-1 receptor. To confer CSF-1-responsiveness, we have expressed the human c fins gene in immortalized and primary T-cells. Addition of soluble CSF-1 resulted in synergistic enhancement of IL-2-driven T-cell proliferation. CSF-1 also co-stimulated the production of interferon (IFN)-gamma by activated T-cells. These effects required Y809 of the CSF-1R and activation of the Ras-MEK-MAP kinase cascade, but were independent of PI3K signalling. T-cells that express c-fins are also responsive to membrane-anchored CSF-1 (mCSF-1) which, unlike its soluble ...
IL-4(38-37)-PE38KDEL is a targeted fusion protein that specifically attaches to cells expressing IL-4Rs reported to be present on many different solid tumor cells of varying pathology (21) . After binding to the IL-4R, the fusion protein is internalized, and then the toxin portion of the molecule causes the ADP ribosylation of elongation factor 2 and arrests protein synthesis (22) . The cell dies by apoptosis attributable to the lack of new protein synthesis (23) . Human brain tumor cells are particularly enriched in the expression of IL-4Rs on their cell surface (9 , 10) . Although numerous preclinical safety and efficacy experiments have been performed with IL-4(38-37)-PE38KDEL, this targeted drug has not been administered to humans before. Here we demonstrate for the first time that intraglioma administration of IL-4(38-37)-PE38KDEL to patients is safe. No treatmentrelated deaths or life-threatening toxicities were observed in any patient. In six of nine patients, administration of ...
Great progress has been made over the past two decades in the development of animal models for ovarian cancer. Each generation of animal model has had its advantages and limitations. The earliest models used a xenograft approach in which human ovarian tumor cells or tissues were grown in immunodeficient mice (17, 27, 32, 33, 36). The xenograft model preserved the complex interactions that occur between cancer cells and their microenvironment, including stromal-epithelial cell interactions, as well as influences of matrix proteins, growth factors, and angiogenesis. Thus, this model was a great advance over cell culture model systems and advanced the study of therapeutic interventions. However, an important weakness in the xenograft model was the lack of host immunity, which severely limited the ability to reliably predict the effect of noncancer immune-host influences on outcomes. In addition, tumors were introduced in the xenograft model rather than arising as primary lesions in the ovary, thus ...
The purpose of this study was to evaluate the effect of plasma-activated media (PAM) on a multicellular tumor spheroid (MCTS) obtained using HCT116 colon carcin...
This unit describes the use of retroviral vectors that can be successfully employed for gene transfer into both primary tumor cultures and established cell lines
CLS weist eine Sammlung von insgesamt 30 verschiedenen Tumorzellen des Gehirns auf (Stand: Dezember 2016). Eine bersicht ber alle derzeitigen Zelllinien isoliert aus Hirntumoren k nnen Sie unter Panel Brain Tumor cell lines. als PDF herunterladen. Die Informationen sind unterteilt in Tabelle 1, die allgemeine Daten enth lt, sowie in Tabelle 2, die Zellmarker, Tumorantigene, Mutationen sowie Zytokine, soweit bekannt, auflistet. ...
CLS weist eine Sammlung von insgesamt 30 verschiedenen Tumorzellen des Gehirns auf (Stand: Dezember 2016). Eine bersicht ber alle derzeitigen Zelllinien isoliert aus Hirntumoren k nnen Sie unter Panel Brain Tumor cell lines. als PDF herunterladen. Die Informationen sind unterteilt in Tabelle 1, die allgemeine Daten enth lt, sowie in Tabelle 2, die Zellmarker, Tumorantigene, Mutationen sowie Zytokine, soweit bekannt, auflistet. ...
Tumor-derived cell lines matched to either normal or metastatic cell lines obtained from the same patient provide a valuable resource for cancer studies. The availability of such models allows researchers to compare tumor lines to their normal counterparts.
Tumor-derived cell lines matched to either normal or metastatic cell lines obtained from the same patient provide a valuable resource for cancer studies. The availability of such models allows researchers to compare tumor lines to their normal counterparts.
Pdpk1 - Pdpk1 (untagged ORF) - Rat 3-phosphoinositide dependent protein kinase-1 (Pdpk1), (10 ug) available for purchase from OriGene - Your Gene Company.
Circulating Tumor Cell (CTC) Diagnostics Market Driven by Increasing incidences of cancer : Size, Share, Trends, Growth and Forecast 2021
To investigate the patterns of genetic lesions in a panel of 23 human multiple myeloma cell lines (HMCLs), we made a genomic integrative analysis involving FISH, and both gene expression and genome-wide profiling approaches. The expression profiles of the genes targeted by the main IGH translocations showed that the WHSC1/MMSET gene involved in t(4;14)(p16;q32) was expressed at different levels in all of the HMCLs, and that the expression of the MAF gene was not restricted to the HMCLs carrying t(14;16)(q32;q23). Supervised analyses identified a limited number of genes specifically associated with t(4;14) and involved in different biological processes. The signature related to MAF/MAFB expression included the known MAF target genes CCND2 and ITGB7, as well as genes controlling cell shape and cell adhesion. Genome-wide DNA profiling allowed the identification of a gain on chromosome arm 1q in 88% of the analyzed cell lines, together with recurrent gains on 8q, 18q, 7q, and 20q; the most frequent ...
It was reported previously that the specific aim of screening agents that would induce apoptosis in the human breast carcinoma cell line MDA-MB-231 was completed, and that calyculin A was the most effective compound tested. Since then Cdc25 phosphatases were reported as possible key oncogenes in human breast carcinoma. In light of our results with calyculin A, it was decided that a more promising focus for the project would be the biochemical basis for the oncogenic actions of Cdc25 phosphatases in human breast carcinoma. At this time we have examined 18 compounds and discovered a Cdc25 phosphatase inhibitor, called 1f, that selectively inhibits Cdc25A,B, and C, has antiproliferative activity against and causes a G1 block in MDA-MB-231 cells. The pure form of this compound and two other compounds which selectively inhibit Cdc25 phosphatases have been made and also demonstrate an equal if not better inhibition of Cdc25 phosphatases as their combinatorial form.*HUMANS
In the present study we describe the establishment and characteristics of a new human tumor cell line (OV-1063) positive for carcinoembryonic antigen (CEA) originating from ovarian metastatic tumor cells. Analysis of the cultured cells during their in vitro adaptation period revealed while the primary culture exhibited a low proportion of CEA-positive cells, this proportion increased with culture passages and eventually more than 90% of the cells in the established line were CEA-positive. Thus, during the period of adaptation to in vitro growth, a selection for CEA-positive cells took place but the amount of CEA secreted per each positive cell seemed to be constant. Several tumor-associated characteristics were found positive on the established OV-1063 cell line. The in vitro growing cell line exhibited an abnormal chromosome pattern with a near-trisomy karyotype for some chromosomes, colony formation in soft agar as well as positive staining with a monoclonal antibody B38.1. Culture supernatants of the
A team of researchers at the Sylvester Comprehensive Cancer Center at the University of Miami Miller School of Medicine has developed a technique for growing ovarian cancer cell lines that mimic the original tumor in long-term cultures. The technique offers hope for quickly advancing precision medicine applications for a variety of cancers by enabling clinicians to test therapies on cells from a patients own tumor in the laboratory, thus identifying the most targeted therapy for that patient. The results of the research, which was led by Tan A. Ince, M.D., Ph.D., associate professor of pathology, Scientific Director of Sylvesters Live Tumor Culture Core and Tissue Bank Core Facility, and Director of the Tumor Stem Cell Division at the Interdisciplinary Stem Cell Institute, have been published online in the journal Nature Communications in an article entitled "Characterization of Twenty-Five Ovarian Tumour Cell Lines that Phenocopy Primary Tumours." The key to Inces research is his development ...
Cell lines are invaluable tools for biomedical research. Cancer cell lines are the foundation of cancer biology and the quest for drug treatments. Adenocarcinoma is a cancer of the epithelium (including colon) and originates in glandular tissue, such as skin surface layer and glands.. Product. These human adenocarcinoma cell lines have been previously licensed to commercial parties for research into human adenocarcinoma treatments.. These cell lines are available through Public Health England ECACC. Please make your purchase through the links below.. Imperial Innovations requests that commercial/for profit parties contact them first to discuss the terms of supply prior to Innovations authorising the release of supplies from ECACC.. ...
MMP-2 production by tumor cells has been demonstrated to play a fundamental role in ECM degradation and tumor cell invasion (for review see 5 . The recent finding of reduced tumor progression in MMP-2-deficient mice (57) highlights the importance of this molecule. In this study, we wished to determine how MMP-2 gene expression is regulated in human astroglioma cells, as a strong correlation has been observed between astroglioma invasion and MMP-2 expression (17, 19, 20, 22, 23, 27). Our results indicate that two cytokines, TNF-α and IFN-γ, partially inhibit MMP-2 gene expression and can function together in an additive manner for near-complete inhibition of MMP-2 expression in human astroglioma cells. TNF-α/IFN-γ inhibition of MMP-2 expression was observed at several levels: on gelatinolytic activity as determined by zymography, on protein expression (both ProMMP-2 and activated MMP-2) as assessed by immunoblotting, on MMP-2 mRNA expression, and on MMP-2 promoter activity. Our results also ...
MDA-MB-435, a member of the NCI-DTP panel of 60 human tumor cell lines, has been used for decades as a model of metastatic human breast cancer. This cell line was derived at M.D. Anderson in 1976 from a pleural effusion from a 31-year old woman with a history of breast cancer (Cailleau R, Olive M, Cruciger QV. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro. 1978 Nov;14(11):911-5.; Brinkley BR, Beall PT, Wible LJ, Mace ML, Turner DS, Cailleau RM. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 1980 Sep;40(9):3118-29.) Further background information on this cell line may be found at the M.D. Anderson Breast Cancer Cell Line Database.. Recent advances in gene expression analysis allow the opportunity to more fully characterize tumor cell lines. Analysis of MDA-MB-435, in conjunction with the rest of the NCI60 panel, revealed that the pattern of gene expression for MDA-MB-435 more closely ...
Data presented above show that the human fibrosarcoma cell line HT1080 is able to inhibit the growth of experimental metastases. These results suggest that this inhibition is due to the antiangiogenic activity of circulating TSP-1 that is released by the tumor cells in vivo, circulates at effective levels, and makes the tumor-bearing animals unable to mount an angiogenic response. The data also demonstrate the in vivo efficacy of soluble TSP-1, showing it can be used as a drug to prevent the growth of experimental metastases.. HT1080 produced concomitant tumor resistance directly by secreting active TSP-1, unlike previously studied rodent tumors that create an antiangiogenic state when the tumor cells, or associated stromal elements (29), secrete enzymes or activators of enzymes (30) that generate inhibitors from other molecules. We saw no significant evidence for in vivo breakdown of tumor-derived TSP-1 to fragments smaller than the 140 kDa monomer, a molecule that is as effective an ...
4Discussion. This study addressed the question of which are the cellular processes that sensitive leukemic cells induced to achieve tolerance to vincristine. To this end, the B-ALL cell line CCRF-SB was gradually exposed until cell proliferation was observed in the presence of 6nM vincristine, and the corresponding proteomic profile was compared to that of cells grown in the absence of the chemotherapeutic drug.. Chemoresistance may be intrinsic or acquired.18 The ability to tolerate high concentrations of chemotherapeutics of an intrinsically resistant cancer cell is not developed as a result of an exposition to the drugs; instead it is the result of genetic abnormalities the cell carries before exposition.18 By contrast, acquired chemoresistance is developed after the cancer cell is exposed to the drug and may be the result of molecular evolution of resistant clones.19 Experimental settings to study acquired chemoresistance include the comparison of matched paired samples at diagnosis and ...
Detail záznamu - Neoplastic progression of the human breast cancer cell line G3S1 is associated with elevation of cytoskeletal dynamics and upregulation of MT1-MMP - Detail záznamu - Knihovna Akademie věd České republiky
To study potential sources of tumor-associated Ags in human ovarian cancer, we have established two ovarian tumor cell lines (OvS1 and OvA2) from two ovarian cancer patients, which express the cellular oncogene HER2/neu. Corresponding tumor infiltrating lymphocyte cultures have also been established and display an autologous tumor-specific pattern of cytotoxicity that is HLA-A2 restricted. To determine the potential relationship between HER2/neu expression and CTL-mediated cytolysis, we first established tumor cell clones from OvS1. These were categorized as high or low expressors of HER2/neu (cOvS1+ or cOvS1-, respectively), and cOvS1+ clones displayed a significantly higher sensitivity to CTL killing as compared with cOvS1- clones. To modulate the expression of HER2/neu, ovarian cancer cells were treated with IFN-gamma. After this exposure, HER2/neu expression was significantly decreased, whereas the expression of HLA Class I was significantly increased. Despite the increase in HLA Class I ...
The cyclin-dependent kinase member, Cdk5, is expressed in a variety of cell types, but neuron-specific expression of its activator, p35, is thought to limit its activity to neurons. Here we demonstrate that both Cdk5 and p35 are expressed in the human astrocytoma cell line, U373. Cdk5 and p35 are present in the detergent-insoluble cytoskeletal fraction of this cell line and Cdk5 localizes to filopodia and vinculin-rich regions of cell-matrix contact in lamellopodia. When exposed to a 46(o)C heat shock, U373 cells change shape, lose cell-matrix contacts and show increased levels of apoptosis. To test whether Cdk5 activation might play a role in these events, U373 cells were stably transfected with histidine-tagged or green fluorescent protein-tagged constructs of Cdk5 or a dominant negative mutation, Cdk5T33. Under normal growth conditions, growth characteristics of the stably transfected lines were indistinguishable from untransfected U373 cells and Cdk5 localization was not changed. However, ...
Amphiregulin (AR) is a newly discovered glycosylated, 84-amino acid residue polypeptide growth regulator which has sequence homology to the EGF family of proteins. To obtain immunological reagents to study the biological role of AR, two synthetic peptides containing sequences corresponding to distinct regions of AR were used to generate polyclonal antibodies in rabbits. One preparation of antipeptide antibodies directed against residues 26-44 of AR (AR-Ab2) was most effective in the detection of native AR, whereas another preparation of antibodies against residues 8-26 (AR-Ab1) was found to be most efficacious in the detection of AR in formalin-fixed and paraffin-embedded tissues. The growth of a colon carcinoma cell line, Geo, which proliferates autonomously under serum-free conditions, was stimulated by the exogenous addition of AR or EGF. Half-maximal stimulation of this growth was observed at 40 and 200 pM of EGF and AR, respectively. A mAb to the extracellular domain of the EGF receptor ...
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Additional comment: After the equilibration, the IPG gel strips were cut to size. Six mm were removed from the anodic end and 14 mm from the cathodic end. The second dimension gels were overlayered with a solution containing agarose (0.5% w/v) and Tris-glycine-SDS (25 mM-198 mM-0.1% w/v) pH 8.3 heated at about 70o C and the IPG gel strips were immediately loaded through it ...
Scale bar: 100 μm. B. The proliferation of atypical tumor cells with osteoid formation is shown. Xenografted tumor cells resemble original tumor cells. Scale bar: 50 μm. Cell growth and morphological findings in vitro UTOS-1 cells were spindle-shaped, contained several nucleoli, and formed clumps. Two weeks after initial cultivation in primary culture, the tumor cells reached subconfluence with some Pictilisib piled-up foci selleck products of cells (Figure 4A). After the cells were serially subcultured for about 3 months, they began to grow rapidly at passage 6 (Figure 4B). Figure 4 Morphology under phase-contrast microscopy. A. In primary. culture, spindle-shaped tumor cells reach subconfluence with some piled-up foci of cells. Scale bar: 100 μm. B. At passage. 6, the tumor cells begin to grow rapidly. The configuration of tumor cells is equalized after the 6th generation. Scale bar: 100 μm. This new cell line has been maintained in vitro for more than 50 passages over more than 2 years. ...
Single Cell Profiling of Circulating Tumor Cells: Transcriptional Heterogeneity and Diversity from Breast Cancer Cell Lines. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Ovarian cancer is a cancer that forms in an ovary. It results in abnormal cells that have the ability to invade or spread to other parts of the body. SK-OV-3, NIH:OVCAR-3 and HO-8910 are the ovarian carcinoma cell lines derived from human ovarian tumor. Studies were carried out to determin
We have shown recently that the hyaluronan receptor, CD44, and matrix metalloproteinase 9 (MMP-9) form a complex on the surface of TA/St mouse mammary carcinoma cells that activates latent transforming growth factor-beta (TGF-beta) and is required for tumor invasion. Disruption of the CD44/MMP-9 com …
MiRNA-21 ASO reduced the colony formation capacity of human colon carcinoma cells. Human colon carcinoma cell line HCT116 cells were transiently transfected wit
TABLE-US-00004 TABLE 4 CTC CTPC Analysis of Cells Migrating from Tumor Cultures Cell Count CTC CTPC Test (Viable/ Clus- Clus- Group Sample Total) CTCs ters CTPCs ters 1 Day 2, 5, 7 ,1%/nd .sup. 70 4 44 0 Pool1,2 Day 9, 13, ,1%/nd .sup. 44 2 20 0 15 Pool1,2 Day 17, 19, 1360/12,600 0 0 4 2 21 Pool1,2 Day 23, 26, 1340/10,800 26 2 30 4 28 Pool1 Day 33 2,230/2,800.sup. 32 0 40 0 Day 35 1,700/8,500.sup. 54 0 46 0 Day 40 190/1,000 74 0 44 0 2 Day 2, 5, 7 ,1%/nd .sup. 166 0 76 2 Pool1,2 Day 9, 13, ,1%/nd .sup. 18 0 16 0 15 Pool1,2 Day 17, 19, 880/2,600 6 0 10 0 21 Pool1,2 Day 23, 26, 740/3,000 0 0 28 4 28 Pool1 Day 33 2,450/2,800.sup. 52 0 48 0 Day 35 1,420/3,500.sup. 62 0 50 0 Day 40 740/2,000 84 2 64 4 3 Day 2, 5, 7 ,1%/nd .sup. 80 0 74 0 Pool1,2 Day 9, 13, ,1%/nd .sup. 12 0 6 0 15 Pool1,2 Day 17, 19, 340/15,600 6 2 20 2 21 Pool1,2 Day 23, 26, 640/10,200 32 2 8 0 28 Pool1 Day 33 3,350/3,500.sup. 140 0 92 0 Day 35 980/2,000 46 0 34 0 Day 40 280/1,500 160 0 122 0 1Individual samples were frozen and ...
TY - JOUR. T1 - Experimental approaches to problems of invasion and metastasis.. AU - Paweletz, N.. AU - Paku, S.. AU - Werling, H. O.. AU - Spiess, E.. PY - 1986/1/1. Y1 - 1986/1/1. N2 - The highly metastasizing ASML cells and the non-metastasizing AS cells, arisen as spontaneous tumors of the rat, were confronted with rat lung tissue in vitro. Small cubes of the lung were allowed to heal their cut edges, then tumor cells were added. Both tumor cell types adapted their shape to the environment, penetrated the superficial layer of lung cells, either of epithelial or of fibroblastoid character and settled on the basal lamina, which, however, was not pierced. In a second set of experiments the tumor cells were inoculated intravenously into the living animal. The lung loaded with tumor cells was excised and cut into cubes which were then incubated in vitro. Here also both tumor cell types exhibited an invasive behavior but the basal lamina of the vessels in which the tumor cells have been arrested ...
The liver is a common site of metastases from a variety of organs such as lung, breast, colon and rectum. When liver metastases occur at the time of initial diagnosis of the primary tumor, they are described as synchronous. If detected after the initial diagnosis, they are described as metachronous. The liver is frequently involved since it receives blood from the abdominal organs via the portal vein. Malignant cells detach from the primary cancer, enter the bloodstream or lymphatic channels, travel to the liver, and grow independently. We do not understand the mechanism of how a tumor cell can leave the primary site and grow in specific organs. Potentially, the environment of the liver is suitable to the growth of certain tumor cells. Once a tumor begins to grow in the liver, it receives its blood supply from the hepatic artery ...
Oligonucleotides having approximately 8 to 18 nucleotide units and a 3-tail which includes asteroid structure attached to the 3-end through the A ring of the steroid skeleton and which form substantially stable duplexes at physiological temperature, have selective cytotoxic activity against certain tumor cell lines.
Buy our Raji (human Burkitts lymphoma cell line) membrane lysate, tumor cell line. ab30126 has been validated in western blot. Abcam now offers…
Cancer cell lines are originally derived from patient tumors, but acquire the ability to proliferate within in vitro cell cultures. As a result of in vitro manipulation, cell lines that have been traditionally used in cancer research undergo genetic transformations that are not restored when cells are allowed to grow in vivo.[9] Because of the cell culturing process, which includes enzymatic environments and centrifugation, cells that are better adapted to survive in culture are selected, tumor resident cells and proteins that interact with cancer cells are eliminated, and the culture becomes phenotypically homogeneous.[4] When implanted into immunodeficient mice, cell lines do not easily develop tumors and the result of any successfully grown tumor is a genetically divergent tumor unlike the heterogeneous patient tumor.[4] Researchers are beginning to attribute the reason that only 5% of anti-cancer agents are approved by the Food and Drug Administration after pre-clinical testing to the lack ...
Discover our worklow for the generation and culture of primary human tumor cell lines, and find out how to retain the primary tumors heterogeneity. - USA
Nik Mohd Afizan Nik Abd Rahman is the author of this article in the Journal of Visualized Experiments: Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures
Acid-Prepared Mesoporous Spheres (APMS) Increase Efficacy of Doxorubicin Transfer and Toxicity in Human Mesothelioma Cells, S. R. Blumen, K. Cheng, M. MacPherson, M. E. Ramos-Nino, T. A. James, D. J. Taatjes, C. C. Landry, and B. T. Mossman, Int.J. Cancer., doi: 10.1002/ijc.25666 ...
Rhomboid protease RHBDL2 is shown to cleave mammalian EGF, producing an active form that stimulates EGFR signalling. EGFR signalling is crucial in development and disease, and endogenous RHBDL2 activity is present in several tumour cell lines, suggesting that it could regulate EGFR signalling in vivo and that one should look beyond ADAMs to see the whole picture. ...
O human hepatoma cells and also virus entry into Huh 7.5 cells in infectious cell culture system.PBS and pelleted at 30,000 rpm for 2 h and stored at 270uC.
Critical Outcome Technologies Inc. released important new test results today proving that COTI-2, the companys lead oncology candidate, stops cancer cells from replicating by correcting the effects of ...
Thought to be genetically stable and identical, cancer cell lines harbor significant levels of genetic variation, which may help explain why it can be hard to reproduce findings in cell line-based research.
PromoCells Primary Cancer Culture System is a standardized, animal component-free and serum-free system for the isolation and culture of human primary tumor cells. It supports the long-term culture of cancer cells while maintaining the original tumor properties and heterogeneity with controlled stroma support. Prolonged culture allows for functional selection of malignant cells giving access to an enriched population of primary cancer cells.. An innovative solution for culturing primary cancer cells: ...
Read user reviews, compare products and contact manufacturers of Cell Lines products, including eukaryote, prokayote and stem cell lines on SelectScience.
Read user reviews, compare products and contact manufacturers of Cell Lines products, including eukaryote, prokayote and stem cell lines on SelectScience.
Circulating tumor cells (CTCs) are very important targets for cancer research. Their detection and molecular characterization enable researchers to gain new insights in the mechanism of cancer. However, detecting them against the background of normal cells in whole blood can be challenging, as it requires selective enrichment of CTCs or removal of other nucleated cells while maintaining the variability of tumor cell expression. ...
In article ,Pine.SOL.3.91.970204092639.10836C-100000 at post,, Rachel Teitelbaum ,teitelba at aecom.yu.edu, wrote: ,There was a while back a treatment I thought was promising, but have lost ,sight of the follow-up. Anyone out there know what happened? They were [description of transfecting B7 into tumor cells] Well, yeah, there are a lot of things you can transfect into a tumor cell line to make it more easily rejected when you inject it into a host (mouse); costimulatory molecules, cytokines, MHC overexpressing constructs, etc. The problem is how to direct the immunity to a pre-existing tumor. I suppose the following approach is theoretically possible: Surgical oncologist removes a tumor from patient x. A sample of the tumor cells is transfected with an immunostimulatory construct, then injected back into the patient. The injected cells might raise specific anti-tumor effector cells, and if the tumor cells were irradiated, they would be prevented from growing themselves. This would be a ...
門川 佳央 , 久保 肇 , 坂井 義治 日本消化器外科学会雑誌 40(5), 693, 2007-05-01 CiNii PDF - オープンアクセス J-STAGE 医中誌Web 参考文献3件 ...
Manassas, VA (PRWEB) February 07, 2012 -- Searching for appropriate tumor cell models often entails a time-intensive review of the literature and genomic
An increasing number of chromosomal aberrations is being identified in solid tumors providing novel biomarkers for various types of cancer and new insights into the mechanisms of carcinogenesis. We applied next generation sequencing technique to analyze the transcriptome of the non-small cell lung carcinoma (NSCLC) cell line H2228 and discovered a fusion transcript composed of multiple exons of ALK (anaplastic lymphoma receptor tyrosine kinase) and PTPN3 (protein tyrosine phosphatase, nonreceptor Type 3). Detailed analysis of the genomic structure revealed that a portion of genomic region encompassing Exons 10 and 11 of ALK has been translocated into the intronic region between Exons 2 and 3 of PTPN3. The key net result appears to be the null mutation of one allele of PTPN3, a gene with tumor suppressor activity. Consistently, ectopic expression of PTPN3 in NSCLC cell lines led to inhibition of colony formation. Our study confirms the utility of next generation sequencing as a tool for the ...
Cell lines are invaluable tools for biomedical research. Cancer cell lines are the foundation of cancer biology and the quest for drug treatments. Adenocarcinoma is a cancer of the epithelium (including colon) and originates in glandular tissue, such as skin surface layer and glands.. Product. These human adenocarcinoma cell lines have been previously licensed to commercial parties for research into human adenocarcinoma treatments.. These cell lines are available through Public Health England ECACC. Please make your purchase through the links below.. Imperial Innovations requests that commercial/for profit parties contact them first to discuss the terms of supply prior to Innovations authorising the release of supplies from ECACC.. ...
TY - JOUR. T1 - Identification of Endogenous Inhibitory Growth Factors from a Human Colon Carcinoma Cell Line. AU - Levine, Alan E.. AU - McRae, L. J.. AU - Hamilton, David A.. AU - Brattain, Diane E.. AU - Yeoman, Lynn C.. AU - Brattain, Michael G.. PY - 1985/5/1. Y1 - 1985/5/1. N2 - A line of human colon carcinoma cells, designated MOSER, was established which synthesized tumor-inhibitory factor (TIF) and transforming growth factor (TGF) activity. Both activities were found in serum-free conditioned medium and in cell extracts. The activities coelute on Bio-Gel P-10 in acetic acid, but can be completely separated by reverse-phase high-pressure liquid chromatography. The TIF and TGF activities were acid and heat stable and were sensitive to trypsin and dithiothreitol. MOSER cell TIF prevented the anchorage-independent growth of the more differentiated colon carcinoma cell lines tested but did not affect the less differentiated lines. Using anchorage-dependent growth conditions, the effect of ...
The low molecular weight thiol (-SH) content of a human prostate carcinoma cell line (LNCap), important to the cellular resistance to drugs and irradiation, was investigated using three forms of thiol assay each utilizing different chemistries. The composition of the mixture was examined by derivatization of the thiols with a three-fold excess of the Ellman reagent to give mixed aromatic disulfides. The components were isolated by chromatography on C18 reverse phase silica gel followed by DE52 anion exchange separation, and then analyzed by capillary electrophoresis against prepared standards. The glutathione adduct (GSSE) and an unknown disulfide (RSSE) were the major components isolated on DE52 together with two minor ones. However, from the absorbance at 325 nm, it was found that the GSSE isolated (1.5 ± 0.2 femtomoles/cell) could only account for 28.5 ± 4.3% of the total ASF thiols. It appeared that the bulk of the thiol material had not formed a stable mixed disulfide with Ellmans reagent, and
1 alpha,25-Dihydroxyvitamin D (1,25 D; also know as calcitriol), the hormonal form of vitamin D, can inhibit the proliferation and promote the differentiation of human prostate adenocarcinoma cells. However, little is known about the effects of 1,25 D on the invasive ability of prostate cancer cells. We used an in vitro bioassay of cell invasion (Amgel assay) to examine the effects of 1,25 D and a "noncalcemic" vitamin D analogue, 1,25-dihydroxy-16-ene-23-yne-cholecalciferol (16-23-D3), on the invasiveness of three well-characterized human prostate carcinoma cell lines: DU 145, PC-3, and LNCaP. PC-3 and LNCaP cells were poorly invasive in Amgel and were hardly affected by treatment with 1,25 D or 16-23-D3 (, 3%). Conversely, DU 145 cells were highly invasive in Amgel, and their invasion was markedly inhibited by 1,25 D and 16-23-D3 (maximally 66 and 59.4% respectively). This effect was both dose-dependent, with maximal inhibition at 1 x 10(-7) M and 72 h. Significant inhibition of invasion was ...
3University of Rochester Cancer Center, Rochester NY 14642, U.S.A.. Correspondence to: B.V. Polevoda, Dept. of Biochemistry and Biophysics, University of Rochester Medical Center, P.O. Box 712, 601 Elmwood Ave., Rochester, NY 14642 U.S.A. Tel 716-275-3329; Fax 716-271-2683. E-mail: [email protected] Key Words: Apoptosis, cell-cycle, G2/M arrest, p27, Ukrain.. Abstract. Exposure of ME 180 and A431 carcinoma cells to Ukrain (NSC-631570), a novel semisynthetic drug from Chelidonium majus L, results in cell growth inhibition which is concomitant with reversible G2/M cell cycle arrest and apoptosis at doses as low as 7 μΜ. In contrast, the same drug concentrations were not affective towards normal human keratinocytes. In order to investigate whether cell cycle control mechanisms are effected in response to Ukrain, we analyzed cell cycle distribution and levels of cyclins and cyclindependent kinases in drug treated carcinoma cells. We found alterations in levels of mitotic cyclins ...
The phosphatidylinositol 3-kinase (PI3K) signaling pathway has inherent oncogenic potential. It is up-regulated in diverse human cancers by either a gain of function in PI3K itself or in its downstream target Akt or by a loss of function in the negative regulator PTEN. However, the complete consequences of this up-regulation are not known. Here we show that insulin and epidermal growth factor or an inactivating mutation in the tumor suppressor PTEN specifically increase the protein levels of hypoxia-inducible factor (HIF) 1alpha but not of HIF-1beta in human cancer cell lines. This specific elevation of HIF-1alpha protein expression requires PI3K signaling. In the prostate carcinoma-derived cell lines PC-3 and DU145, insulin- and epidermal growth factor-induced expression of HIF-1alpha was inhibited by the PI3K-specific inhibitors LY294002 and wortmannin in a dose-dependent manner. HIF-1beta expression was not affected by these inhibitors. Introduction of wild-type PTEN into the PTEN-negative ...
International Scholarly Research Notices is a peer-reviewed, Open Access journal covering a wide range of subjects in science, technology, and medicine. The journals Editorial Board as well as its Table of Contents are divided into 108 subject areas that are covered within the journals scope.
Multicellular tumor spheroid cultures (MCTS), have a wide variety of uses in the field of cancer treatment. Current methods, however, do not provide spheroids adequate large for therapy testing. In order to circumvent this problem, a bioreactor made using Polydimethylsiloxane (PDMS) was constructed to allow the adequate growth of spheroid clusters. Liver Hepatic Carcinoma cells (Hep G2) and Anaplastic Thyroid Carcinoma (SW 1736) were cultured and isolated. They were then further matured using the "hanging drop" method, involving spheroid formation using gravity. The optimal growth time for hanging drop cultures was determined to be 72 hours. PDMS wells of different diameter were then constructed using a 24-well plate as a base, and clusters of cells were transferred into them for MCTS formation. The wells were fabricated using PDMS as a mold, then carving out wells for cell growth. Development of the spheroids in the bioreactor was monitored using microscopy paired with various staining ...
Prostaglandins can be synthesized in an adrenocortical carcinoma, and they can work in an autocrine or paracrine fashion. In rabbit chondrocyte and human squamous carcinoma cell lines, EGF induced the secretion of PGE2 via up-regulation of the activities of phospholipase A2 (PLA2) and COX-2 (Sato et al. 1997, Huh et al. 2003). This may suggest that PGE2 acts as a secondary factor to EGF in the up-regulation of aromatase expression. Therefore, we checked whether PGE2 was secreted from NCI-H295R cells in response to EGF. In this study, NCI-H295R cells secreted PGE2 in response to EGF (Fig. 13), and PGE2 increased aromatase activity to a greater extent than other prostaglandins (Fig. 6). The inhibition of EGF-induced aromatase expression with PGE2 receptor antagonists confirmed that PGE2 is the secondary factor of aromatase expression with EGF (Fig. 14). PGE1 also increased aromatase activity to a degree similar to that of PGE2, but EGF could not stimu- late NCI-H295R cells to secrete a sufficient ...
Autophagy plays an important role in cancer because of its tumour suppressing and tumour protecting function. For tumour suppressing function at the initiation stage, ATG Beclin-1 (Fig. 1) was identified as a tumour suppressor gene as it is mono-allelically deleted in many cases including ovarian cancers (75%), breast cancers (50-70%) and prostate cancers (40%) [5]. Also, Beclin-1 is allelically deleted and weakly expressed in most human breast carcinoma cell lines while the normal epithelium cells demonstrated a much higher expression [6]. In addition, overexpression of Beclin-1 in human breast carcinoma cell line MCF-7 cells could reduce tumourigenesis by inhibiting cell proliferation in a xenograft model [2]. Thus, low expression of Beclin-1 could favour the development of cancer. For colorectal and gastric cancers, associations were found with the down-regulation of Bif-1 and Atg2B, Atg5, Atg9B and Atg12 mutations, which led to inhibition of programmed cell death in colon cancer (Fig. 1). ...
Recent studies have indicated a link between levels of cyclooxygenase-2 (COX-2) and development of the multidrug resistance (MDR) phenotype. The ATP-binding cassette sub-family G member 2 (ABCG2) is a major MDR-related transporter protein that is frequently overexpressed in cancer patients. In this study, we aimed to evaluate any positive correlation between COX-2 and ABCG2 gene expression using the COX-2 inducer 12-O-tetradecanoylphorbol-13-acetate (TPA) in human breast cancer cell lines. ABCG2 mRNA and protein expression was studied using real-time RT-PCR and flow cytometry, respectively. A significant increase of COX-2 mRNA expression (up to 11-fold by 4 h) was induced by TPA in MDA-MB-231 cells, this induction effect being lower in MCF-7 cells. TPA caused a considerable increase up to 9-fold in ABCG2 mRNA expression in parental MCF-7 cells, while it caused a small enhancement in ABCG2 expression up to 67 % by 4 h followed by a time-dependent decrease in ABCG2 mRNA expression in MDA-MB-231 cells.
Principal Investigator:YAMAGUCHI Koji, Project Period (FY):2000 - 2001, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Digestive surgery
Fibroblast growth factors (FGFs) and their high-affinity receptors contribute to the autocrine growth stimulation in several human malignancies. Here, we describe that FGF18 expression is up-regulated in 34/38 colorectal tumours and is progressively enhanced during colon carcinogenesis reaching very high levels in carcinoma. Moreover, our data suggest that FGF18 affects both tumour cells and tumour microenvironment in a pro-tumorigenic and pro-metastatic way. Addition of recombinant FGF18 to the culture media of slowly growing colorectal tumour cell lines LT97 and Caco-2 stimulated proliferation. Phosphorylation of externally regulated kinase 1/2 and S6 was increased already 5 min after growth factor addition. SW480 cells, endogenously producing large amounts of FGF18, were not affected in this setting, but recombinant FGF18 supported tumour cell survival under conditions of serum starvation. Down-modulation of endogenous FGF18 production by small interference RNA (siRNA) significantly reduced ...
Many types of mammalian cells can aggregate and differentiate into 3-D multicellular spheroids when cultured in suspension or a nonadhesive environment. Compared to conventional monolayer cultures, multicellular spheroids resemble real tissues better in terms of structural and functional properties. Multicellular spheroids formed by transformed cells are widely used as avascular tumor models for metastasis and invasion research and for therapeutic screening. Many primary or progenitor cells on the other hand, show significantly enhanced viability and functional performance when grown as spheroids. Multicellular spheroids in this aspect are ideal building units for tissue reconstruction. Here we review the current understanding of multicellular spheroid formation mechanisms, their biomedical applications, and recent advances in spheroid culture, manipulation, and analysis techniques. ...
The nature of the signal transducing the change in cell volume to the activation of osmolyte efflux pathways for RVD in osmotically swollen cells is not clear. Two major models have been proposed, one involving Ca2+ as an intracellular second messenger and the other in which RVD is mediated through Ca2+-independent mechanisms. According to the first model, cell swelling causes an increase in cytosolic Ca2+ that activates volume regulatory mechanisms. The increase in [Ca2+]i may result from Ca2+ influx, via plasma membrane channels (Christensen, 1987), or Ca2+ release from intracellular stores (McCarty and ONeil, 1992; Wu et al., 1997). The proposed effectors are mostly Ca2+-activated cation and anion channels and carriers. This model has been widely accepted as a paradigm for the involvement of Ca2+ as a transducing signal for RVD. Nevertheless, the existing evidence backing up this model is often weak and fragmentary, and the comprehensive analysis necessary to establish an active role of ...
TY - JOUR. T1 - Association of HER2/neu expression with sensitivity to tumor-specific CTL in human ovarian cancer. AU - Yoshino, Ichiro. AU - Peoples, George E.. AU - Goedegebuure, Peter S.. AU - Maziarz, Richard. AU - Eberlein, Timothy J.. PY - 1994/3/1. Y1 - 1994/3/1. N2 - To study potential sources of tumor-associated Ags in human ovarian cancer, we have established two ovarian tumor cell lines (OvS1 and OvA2) from two ovarian cancer patients, which express the cellular oncogene HER2/neu. Corresponding tumor infiltrating lymphocyte cultures have also been established and display an autologous tumor-specific pattern of cytotoxicity that is HLA-A2 restricted. To determine the potential relationship between HER2/neu expression and CTL-mediated cytolysis, we first established tumor cell clones from OvS1. These were categorized as high or low expressors of HER2/neu (cOvS1+ or cOVS1-, respectively), and cOvS1+ clones displayed a significantly higher sensitivity to CTL killing as compared with ...
Previously, we have demonstrated that interleukin-4 receptor α (IL-4Rα) is overexpressed on a variety of human cancers and can serve as target for IL-4 immunotoxin comprised of IL-4 and a mutated Pseudomonas exotoxin. However, its expression and association with grade and clinical stage of bladder cancer has not been studied. IL-4Rα expression was examined in human bladder cancer cell lines, mouse xenografts, and biopsy specimens at mRNA and protein levels by real-time RT-PCR and IHC/ISH techniques. We also examined the effect of IL-4 on proliferation and invasion of bladder carcinoma cell lines. For tissue microarray (TMA) results, we analyzed the precision data using exact binomial proportion with exact two-sided P-values. We used Cochran-Armitage Statistics with exact two-sided P-values to examine the trend analysis of IL-4Rα over grade or stage of the bladder cancer specimens. The influence of age and gender covariates was also analyzed using multiple logistic regression models. IL-4Rα ...
We sought to optimize the conditions under which B7-mediated gene transfer could effect antitumor immunity. Transduction of murine mammary carcinoma cell lines and human breast cancer cell lines with adenoviruses expressing B7-1 led to high levels of B7 expression. The expression - of mB7-1 on melanoma cells but not mammary carcinoma cells results in reduced growth rate. Interestingly, adenovims-mediated expression of mB7-1 on mammary carcinoma cells did not result in a reduced tumor growth rate.*GENES
Circulating tumor cells (CTCs) have been described as a population of cells that may seed metastasis, which is a reliable target for the prevention of metastases in lung cancer patients at the early stage. The culturing of CTCs in vitro can be used to study the mechanism of lung cancer metastasis and to screen antimetastasis drugs. This study aims to establish CTC cell line in vitro and explore the potential mechanism of its metastasis. A mixture of EpCAM- and EGFR-coated immunomagnetic microbeads in microfluidic Herringbone-Chip was used to capture CTCs. The CTCs, 95-D and A549 cells was evaluated by cell proliferation assays, clonal formation assays, migration assays and drug resistance. Flow cytometry and cytokine protein chip were used to detect the difference in phenotype and cytokine secretion between CTCs, 95-D and A549 cells. The NOD/SCID mice were used to study tumorigenicity, lung organ colonization and metastasis of CTCs. The H&E staining, immunohistochemistry and immunofluorescence assay
Riboflavin (vitamin B2) is a precursor for coenzymes involved in energy production, biosynthesis, detoxification, and electron scavenging. Previously, we demonstrated that irradiated riboflavin (IR)...
The role of estrogen in the growth and survival of ovarian cancer cells is controversial. In this study, we investigated the changes in cell-cycle regulatory proteins in ovarian cancer cell lines after estrogen treatment to explore the role of estrog
Description The Caov-4 cell line is an ovarian cancer cell line with epithelial morphology that grows in adherent culture. These cells harbor a loss-of-function mutation in the p53 gene and are sensitive to cisplatin. Source This cell line was established from a metastatic site (fallopian tube) in a 45-year-old Caucasian female with adenocarcinoma of the ovary. Inventors Jorgen Fogh, PhD, formerly at Sloan Kettering Institute, Memorial Sloan Kettering