As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.

Two new crystal structures of Bacillus stearothermophilus tryptophanyl-tRNA synthetase (TrpRS) afford evidence that a closed interdomain hinge angle requires a covalent bond between AMP and an occupant of either pyrophosphate or tryptophan subsite. They also are within experimental error of a cluster of structures observed in a nonequilibrium molecular dynamics simulation showing partial active-site assembly. Further, the highest energy structure in a minimum action pathway computed by using elastic network models for Open and Pretransition state (PreTS) conformations for the fully liganded TrpRS monomer is intermediate between that simulated structure and a partially disassembled structure from a nonequilibrium molecular dynamics trajectory for the unliganded PreTS. These mutual consistencies provide unexpected validation of inferences drawn from molecular simulations.

Enterovirus 71 (EV-A71) receptors that have been identified to date cannot fully explain the pathogenesis of EV-A71, which is an important global cause of hand-foot-and-mouth disease and life-threatening encephalitis. We identified an interferon-gamma (IFNγ)-inducible EV-A71 cellular entry factor, human tryptophanyl-tRNA synthetase (hWARS), using genome-wide RNAi library screening. The importance of hWARS in mediating virus entry and infectivity was confirmed by virus attachment, in vitro pull-down, antibody/antigen blocking, and CRISPR/Cas9. Upon IFNγ treatment, induced hyperexpression and plasma membrane translocation of hWARS were observed, which sensitized semi-permissive (human neuronal NT2)/non-permissive (mouse fibroblast L929) cells to EV-A71 infection. Our hWARS-transduced mouse infection model showed pathological changes similar to patients with severe EV-A71 infection. The expression of hWARS is also required for productive infection by other human enteroviruses, including the ...

1MB2: Interconversion of ATP binding and conformational free energies by tryptophanyl-tRNA synthetase: structures of ATP bound to open and closed, pre-transition-state conformations.

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Secreted tryptophanyl-tRNA synthetase as a primary defence system against infection / Y H Ahn; S Park; J J Choi; B K Park; K H Rhee; E Kang; S Ahn; Chul Ho Lee; J S Lee; K S Inn; M L Cho; S H Park; K Park; H J Park; J H Lee; J W Park; N H Kwon; H Shim; B W Han; P Kim; J Y Lee; Y Jeon; J W Huh; M Jin; S Kim , 2016 ...

Mouse Monoclonal Anti-Tryptophanyl tRNA synthetase Antibody (3A12) [PE]. Validated: WB, ELISA, ICC/IF, IP. Tested Reactivity: Human. 100% Guaranteed.

Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG.

Compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.

TABLE-US-00002 TABLE 2 Acces- Clear Endo- Fold- Pattern sion Protein Name Cell metrioid Serous Mucinous change t-test C (+) P60174 Triosephosphate isomerase 35.59 15.33 16.76 12.73 2.32 4.41E-08 C (+) P23381 Tryptophanyl-tRNA synthetase, cytoplasmic 6.23 1.24 1.99 1.00 4.20 8.10E-07 C (+) O75223 Uncharacterized protein C7orf24 5.95 1.67 1.27 2.74 3.41 1.68E-02 E (+) Q6P1P0 ASRGL1 protein 2.91 10.03 3.18 3.08 3.30 7.23E-04 E (+) P20472 Parvalbumin alpha 3.85 11.75 1.52 1.52 4.90 2.17E-02 S (+) Q15121 Astrocytic phosphoprotein PEA-15 1.00 2.78 4.79 1.00 3.79 7.25E-03 S (+) O43598 c-Myc-responsive protein Rcl 3.23 2.86 6.87 3.58 2.17 1.81E-04 S (+) R42771 Cyclin-dependent kinase inhibitor 2A 1.35 1.00 4.32 1.98 3.20 1.07E-04 S (+) P52907 F-actin capping protein subunit alpha-1 15.72 20.01 25.55 20.13 1.41 2.14E-03 S (+) O76070 Gamma-synuclein (Breast cancer-specific gene 1 protein) 1.34 1.34 8.25 1.34 6.16 1.76E-03 S (+) Q9HC38 Glyoxalase domain-containing protein 4 2.07 1.68 6.35 1.00 3.71 ...

Peptide chain release factors (RFs) are required for the termination of protein biosynthesis [1]. At present two classes of RFs can be distinguished. Class I RFs bind to ribosomes that have encountered a stop codon at their decoding site and induce release of the nascent polypeptide. Class II RFs are GTP-binding proteins that interact with class I RFs and enhance class I RF activity. In prokaryotes there are two class I RFs that act in a codon specific manner [2]: RF-1 (gene prfA) mediates UAA and UAG-dependent termination while RF-2 (gene prfB) mediates UAA and UGA-dependent termination. RF-1 and RF-2 are structurally and evolutionary related proteins which have been shown [3] to make up a family that also contains the following proteins: ...

SWISS-MODEL Repository entry for A0A4P9T2X5 (A0A4P9T2X5_SALET), Peptide chain release factor 1. Salmonella enterica subsp enterica serovar 1,4,[5],12:i:-

ETF1 recognizes the stop codon in the A site of the ribosome and promotes nascent peptide chain release, and the GTPase GSPT1 facilitates this peptide release via its interaction with ETF1 ...

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This protein is identical to GenBank accession AAD18266 (genome position accession AE001598). The peptide chain release factor 1 (RF-1) directs the termination of translation in response to the peptide chain termination codons UAG and UAA. RF-2 (gene prfB) mediates UAA and UGA-dependent termination ...

Accepted name: serine tRNA ligase. Reaction: ATP + L-serine + tRNASer = AMP + diphosphate + L-seryl-tRNASer. Other name(s): seryl-tRNA synthetase; SerRS; seryl-transfer ribonucleate synthetase; seryl-transfer RNA synthetase; seryl-transfer ribonucleic acid synthetase; serine translase. Systematic name: L-serine:tRNASer ligase (AMP-forming). Comments: This enzyme also recognizes tRNASec, the special tRNA for selenocysteine, and catalyses the formation of L-seryl-tRNASec, the substrate for EC 2.9.1.1, L-seryl-tRNASec selenium transferase.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9023-48-7. References:. 1. Katze, J.R. and Konigsberg, W. Purification and properties of seryl transfer ribonucleic acid synthetase from Escherichia coli. J. Biol. Chem. 245 (1970) 923-930. [PMID: 4906848]. 2. Makman, M.H. and Cantoni, G.L. Isolation of seryl and phenylalanyl ribonucleic acid synthetases from bakers yeast. Biochemistry 4 (1965) 1434-1442.. 3. Webster, L.T. and ...

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Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.

wrt-10 encodes a hedgehog-like protein, with an N-terminal signal sequence, a Wart domain, and a C-terminal region of low-complexity sequence. WRT-10 is expressed in both male and hermaphrodite intestine. the Wart domain is predicted to form a cysteine-crosslinked protein involved in intercellular signalling, and it has subtle similarity to the N-terminal Hedge domain of HEDGEHOG proteins. WRT-10 is required for normal growth to full size and locomotion. both of these requirements may reflect common defects in cholesterol-dependent hedgehog-like signalling or in vesicle trafficking. [Source: WormBase]wrt-10 encodes a hedgehog-like protein, with an N-terminal signal sequence, a Wart domain, and a C-terminal region of low-complexity sequence. WRT-10 is expressed in both male and hermaphrodite intestine. the Wart domain is predicted to form a cysteine-crosslinked protein involved in intercellular signalling, and it has subtle similarity to the N-terminal Hedge domain of HEDGEHOG proteins. WRT-10 is ...

SWISS-MODEL Repository entry for A0A3V7VXR1 (A0A3V7VXR1_SALET), Peptide chain release factor 1. Salmonella enterica subsp enterica serovar Paratyphi B

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The aminoacyl-tRNA synthetases (EC 6.1.1.) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices, and are mostly dimeric or multimeric, containing at least three conserved regions. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2-hydroxyl of the tRNA, while, in class II reactions, the 3-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, ...

The JSU web identity standards include required and recommended components. These standards apply to all sites revamped or created as part of the JSU website redesign and development in 2012, and to all sites created thereafter. For sites outside the scope of the project, we strongly encourage JSU to incorporate the standards as soon as possible.. ...

Define identity element. identity element synonyms, identity element pronunciation, identity element translation, English dictionary definition of identity element. n. The element of a set of numbers that when combined with another number in a particular operation leaves that number unchanged. For example, 0 is the...

Mathew Shane, William Liefert, Mitch Morehart, May Peters, John Dillard, David Torgerson, and William Edmondson. WRS-09-02, March 30, 2009 The world economic crisis that began in 2008 has major consequences for U.S. agriculture. The weakening of global demand because of emerging recessions and declining economic growth result in reduced export demand and lower agricultural commodity prices, compared with those in 2008. These, in turn, reduce U.S. farm income and place downward pressures on farm real estate values. So far, the overall impact on U.S. agriculture is not as severe as on the broader U.S. economy because the record-high agricultural exports, prices, and farm income in 2007 and 2008 put U.S. farmers on solid financial ground. Moreover, the debt equity ratios in agriculture tend to be more conservative than those in most other sectors of the economy. There is much uncertainty concerning the depth and extent of the crisis. The outcomes for U.S. agriculture are dependent on whether or not ...

Arginyl tRNA synthetase (ArgRS), a class I aminoacyl tRNA synthetase, is a monomer which aminoacylates the 2-OH of the nucleotide at the 3 of the appropriate tRNA. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. There are at least three subgroups of ArgRS. One type contains both characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding. The second subtype lacks the KMSKS motif; however, it has a lysine N-terminal to the HIGH motif, which serves as the functional counterpart to the second lysine of the KMSKS motif. A third group, which is found primarily in archaea and a few bacteria, lacks both the KMSKS motif and the HIGH loop lysine [PUBMED:11106639].. ...

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The solution delivered exceeded the criteria outlined, operating as a preliminary screening tool it allows management to identify up to 30 individuals at any one time with a raised temperature as they enter the building. Live alerts sent to the operator enables immediate action to be taken before contact with others occurs.. The system is one of the most accurate on the market, operating within parameters of 0.3 degrees which meet international clearance. In addition, the software allows Eaton to record an audit trail during activations, including steps taken to assist with health, safety and welfare compliance.. WRS Engineers preconfigured the system for MEDC Sutton in Ashfield and a number of other Eaton sites across the UK. The result of this was sites were able to set up the camera, black body calibrator and monitor with ease and had WRS engineering support on hand when required.. Ultimately, the system gives Eaton exactly what they were looking for - a solution that compliments their ...

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Ata hai pouco, non se coñecían marcadores bioquímicos ou moleculares que servisen para distinguir as especies do filo Thermotogae do resto das bacterias.[1] Porén, un estudo xenómico comparativo recente identificou unha gran cantidade de indeis sinatura consevados en importantes proteínas, que son específicos de todas as especies de Thermotogae ou dalgúns dos subgrupos.[19] Dezaoito destes indeis conservados en importantes proteínas como Pol1, RecA, TrpRS e as proteínas ribosómicas L4, L7/L12, S8, S9 etc. só están presentes en especies de Thermotogae secuenciadas, o que proporciona novos marcadores moleculares para este filo. Ademais, estes estudos identificaron 14 indeis conservados que son específicos do clado formado polos xéneros Fervidobacterium e Thermosipho, 12 indeis conservados específicos do xénero Thermotoga (excepto na especie Thermotoga lettingae), e 8 indeis conservados que serven de marcadores moleculares para especies do xénero Thermosipho.[19] O clado formado ...