TY - JOUR. T1 - Disseminated Trichosporon beigelii (cutaneum) Infection in an Artificial Heart Recipient. AU - Murray Leisure, Katherine A.. AU - Aber, Robert. AU - Rowley, Lawrence J.. AU - Applebaum, Peter C.. AU - Wisman, Craig B.. AU - Pennock, John L.. AU - Pierce, William S.. PY - 1986/12/5. Y1 - 1986/12/5. N2 - A 44-year-old man with end-stage ischemic cardiomyopathy was supported with an intra-aortic balloon and The Penn State Heart (artificial) prior to orthotopic cardiac transplantation on the 14th hospital day. At the time of transplantation, intraoperative cultures of pericardial and mediastinal fluid showed growth of Trichosporon beigelii (cutaneum). Shortly thereafter the patient developed visceral dissemination of T beigelii with no associated skin lesions. He was treated with amphotericin B and rifampin, but postmortem examination showed persistent, disseminated infection.. AB - A 44-year-old man with end-stage ischemic cardiomyopathy was supported with an intra-aortic balloon ...
Phosphorylation of acidic ribosomal proteins P1/P2-P0 is a common phenomenon in eukaryotic organisms. It was found previously that in Trichosporon cutaneum, unlike in other yeast species, in addition to the two acidic ribosomal proteins, two other proteins of 15 kDa and 19 kDa of the small ribosomal subunit were phosphorylated. Here we describe two protein kinases: CKI and CKII, which are engaged in the modification of T. cutaneum ribosomal proteins. The acidic ribosomal proteins and the protein of 19 kDa were modified by CKII associated with ribosomes, while the protein of 15 kDa was modified by CKI. Protein kinase CKI was purified from cell-free extract (CKIC) and from ribosomal fraction (CKIR). The molecular mass of CKIC was established at 33 kDa while that of CKIR at 35-37 kDa. A protein of 40 kDa copurified with CKIR but not CKIC. Heparin significantly increased 40 kDa protein phosphorylation level by CKIR. Microsequencing analysis revealed the presence of CKI recognition motifs in the ...
A yeast isolate revealing unique enzymatic activities and substrate-dependent polymorphism was obtained from autochthonous microflora of soil heavily polluted with oily slurries. By means of standard yeast identification procedures the strain was identified as Trichosporon cutaneum. Further molecular PCR product analyses of ribosomal DNA confirmed the identity of the isolate with the genus Trichosporon. As it grew on methanol as a sole carbon source, the strain appeared to be methylotrophic. Furthermore, it was also able to utilize formaldehyde. A multi-substrate growth potential was shown with several other carbon sources: glucose, glycerol, ethanol as well as petroleum derivatives and phenol. Optimum growth temperature was determined at 25°C, and strong inhibition of growth at 37°C together with the original soil habitat indicated lack of pathogenicity in warm-blooded animals and humans. The unusually high tolerance to xenobiotics such as diesel oil (,30 g/l), methanol (50 g/l), phenol (2 ...
The inhibitors present in dilute acid-treated lignocellulosic hydrolysates would show great effect on the growth and product formation of microorganisms. To understand their inhibitory law and mechanism on oleaginous microorganism could help improving the efficiency of lignocellulose hydrolysis, detoxification, and lipid fermentation. The effects of four representative alcohol compounds present in lignocellulosic hydrolysates, including furfuryl alcohol, vanillyl alcohol, catechol, hydroquinone on the cell growth and lipid accumulation of Trichosporon fermentans were systematically investigated in this work. The toxicity of selected alcohol compounds was well related to their log P value except furfuryl alcohol, whose log P value was the minimum but with the highest toxicity to T. fermentans. The inhibition of all the alcohol compounds on the growth of T. fermentans was more serious than on the lipid synthesis. Also, the growth of T. fermentans was more sensitive to the variation of inoculum size,
Trichosporonasahii is a basidiomycete yeast responsible for white piedra and onychomycosis in the immunocompetent host. In the immunocompromised patients, invasive infections are reported; their diagnosis is difficult and they are associated with high mortality rate. Urinary infection due to Trichosporon Asahi is rare butits incidenceis increasing. We report the case of a 58 year old diabetic patient. The yeast was isolated from urine samples of three consecutive crops in pure form. The patient improved after antifungal therapy.
0047] Unexpectedly, in these methods and processes, melt index parameters such as MI and HLMI of the olefin polymer can decrease as the amount of the alcohol compound added to the polymerization reactor system is increased. Also unexpectedly, molecular weight parameters such as Mw and Mz of the olefin polymer can increase as the amount of the alcohol compound added to the polymerization reactor system is increased. The alcohol compound can be added to the polymerization reactor system (e.g., into a polymerization reactor) alone, with a carrier, with the dual catalyst system, etc. The amount of the alcohol compound added to the reactor system is not particularly limited, so long as the amount of the alcohol compound added to the reactor system is sufficient to impact at least one of the MI, HLMI, Mw, and Mz of the olefin polymer as described herein, and does not adversely impact the catalyst activity or polymer production rate in a significant manner (e.g., 20%+ reduction in catalyst activity, ...
Kocho and Bula are fermented product of Enset (Ensete ventricosum). It is the staple food for 20 million people in Ethiopia. The aim of study was to isolate, identify and characterize yeast species from fermented kocho and bulla by using Biolog Micro station. 300 Kocho samples were collected from Angacha District. 0.1ml of serially diluted samples were Streaked on yeast pepton dextrose agar and incubated at 280C. Pure yeast colony inoculum were prepared at 9ml distilled water at 49% +2 turbidometer and transferred in to YT micro plate. Incubated for 24-72 hours at 28oC and micro plate reading were carried out using MicroLog 3 Software version. 4.20.05. Seven yeast species were identified from study samples. Biolog Micro station 100% probability and |0.5 Similarity read identify Cryptococcus albidus Var aerus, Guilliermondella selenospora, Rhodotorula acheniorum and Trichosporon beigelii. 99% Cryptococcus terreus A, 98% Candida zylandase, 86% Kluyveramyces delphensis respectively. Characterization of
Lyman, C.A., Devi, S.J., Nathanson, J., Frasch, C.E., Pizzo, P.A. and Walsh, T.J. (1995) Detection and Quantitation of the Glucuronoxylomannan-Like Polysaccharide Antigen from Clinical and Nonclinical Isolates of Trichosporon beigelii and Implications for Pathogenicity. Journal of Clinical Microbiology, 33, 126-130.
摘要 旨在调查大熊猫体表酵母菌种属及其对小鼠的致病性。通过传统分离培养、形态学观察,并利用真菌ITS序列通用引物ITS1、ITS4对分离株进行PCR扩增和系统发育学分析,从而鉴定其种属。利用丹麦Rosco纸片扩散法,用两性霉素B、制霉菌素、酮康唑、氟康唑、伊曲康唑对12种分离株进行药敏试验和耐药性分析,同时进行动物致病性试验。结果本次共在37个熊猫被毛样本中分离到45株酵母菌,分属于3个属,12个种。酵母分离菌分别为毛孢子菌属(Trichosporon)40株,隐球菌属(Cryptococcus)4株,假丝酵母菌属(Candida)1株,分别鉴定为皮肤毛孢子菌(Trichosporon cutaneum)、串珠毛孢子菌(Trichosporon moniliiforme)、指间毛癣菌(Trichosporon interdigitale)、秋吉台毛孢子菌(Trichosporon akiyoshidainum)、星形毛孢子菌(Trichosporon asteroids)、芸苔毛孢子菌(Trichosporon ...
Background and Purpose: Trichosporon is a genus of anamorphic basidiomycetous yeast which is widely distributed in nature and is found in tropical and temperate areas. The aim of this work was to study the isolation, identification and molecular analysis of Trichosporon species in soil. Materials and Methods: In order to isolate and identify Trichosporon species in soil, 30 samples were collected from 30 different locations across Iran. The isolates were identified by means of the standard methods of yeast identification. To confirm morphological identification, genomic DNA was extracted and the hypervariable D1/D2 domain of the large-subunit (LSU) ribosomal DNA (rDNA) gene was amplified by polymerase chain reaction (PCR), using primer pair NL-1/NL-4, and then the sequences were analyzed. Results: According to the morphological and physiological assessments, isolates were identified as T. coremiiforme. The isolates formed chlamydospore after one week on yeast-malt (YM) agar medium. Using Blast program,
Consider the cumulative impact of this species on the animals, fungi, microbes, and other organisms in the communities that it invades. Although a non-native species may provide resources for one or a few native species (e.g. by providing food, nesting sites, etc.), the ranking should be based on the species net impact on all native species. Give more weight to changes in composition and interactions involving rare or keystone species or rare community types ...
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The species page of Trichosporon montevideense JCM 9937. Also known as Endomycopsis montevidensis. Information about genome files, completeness, GC-content, size, N50-values, and sequencing methods are listed.
The species page of Trichosporon asahii var. asahii CBS 2479. . Information about genome files, completeness, GC-content, size, N50-values, and sequencing methods are listed.
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Several other pediatric dermatoses were diagnosed, such as pediculosis in 18.6% and chickenpox in 15.1%. Among the examined professionals white piedra was not diagnosed. DISCUSSION. Trichosporon spp. (old denomination T. beigelii) is commonly found in nature (soil, water and vegetal matter) and it has been isolated from monkeys, horses and dogs3,6. The diagnosis of Trichosporon infection has became more important in the last few years, as it has been described as eczematous form mimicking dermatophytosis, as generalized papules and nodules in immunocompromised patients representing a trichosporonosis form, or as systemic infection in patient submitted to peritoneal dialysis or parenteral nutrition12,14. White piedra can be clinically indistinguishable from pediculosis or trichobacteriosis, and association of white piedra with bacteria and nits has been observed7,15,16,19. In our casuistic, in one case there was association with pediculosis but we didnt observe association with bacteria by ...
Invasive trichosporonosis is an extremely rare mycosis, but Trichosporon fungemia (TF) in patients with hematologic malignancies has been increasingly recognized to be a fulminant and highly lethal infection. Although the utility of azole therapy has been demonstrated in several observations, little is known about the efficacy of one of azoles, miconazole (MCZ). To assess its therapeutic role, we retrospectively investigated 6 cases of TF in patients with acute leukemia receiving MCZ containing regimens. Successful outcome was obtained in 4 patients [MCZ + amphotericin B (AmB) in 2, MCZ only and MCZ + fluconazole (FLCZ) + AmB in one each], but not in 2 (MCZ + FLCZ + AmB and MCZ + FLCZ in one each). Although MCZ and AmB exhibited good in vitro activities against isolates from all patients, FLCZ had such finding from only one patient. Considering the reportedly limited utility of AmB, MCZ seemed to play a critical role even in the combination therapies for TF. Despite the release of newer
Association between pulmonary disease and IgA nephropathy (IgAN) has been previously reported. However, no association has been reported between hypersensitivity pneumonitis (HP) and IgAN. Here, we report about a patient with no particular medical history, who experienced worsening dyspnoea in the course of 1 month, with ground-glass opacity on chest CT and no improvement after antibiotic therapy. The patient was diagnosed as having HP based on the history of antigen exposure, detection of Trichosporon asahii-specific antibodies and bronchoscopy findings. Concomitantly, findings of renal biopsy revealed the IgAN diagnosis. The patient underwent corticosteroid therapy, with good outcomes for both HP and IgAN. This is the first report in the literature to describe summer-type HP complicated with IgAN. ...
In this work, methanol in crude glycerol solution was used to assist the lipid production with oleaginous yeast Trichosporon oleaginosus cultivated under non-sterilized conditions. The investigated methanol concentration was 0%, 1.4%, 2.2%, 3.3% and 4.4% (w/v). The results showed that methanol played a significant role in the non-sterilized fermentation for lipid production. The optimal methanol concentration was around 1.4% (w/v) in which the growth of T. oleaginosus was promoted and overcame that of the contaminants. The non-sterilized fed-batch fermentation with initial methanol concentration of 1.4% (w/v) was then performed and high biomass production (43.39 g/L) and lipid production (20.42 g/L) were achieved.. ...
La piedra negra áurea es una variante de la piedra negra que puede dar pepitas de oro o al ser minada. 1 Obtención 1.1 Generación natural 1.2 Rotura 2 Uso 2.1 Piglins 3 Curiosidades 4 Referencias La piedra negra áurea se genera como parte de los restos de bastión y se puede encontrar debajo de los cofres del bastión. La piedra negra áurea requiere que se extraiga con un pico, en cuyo .... Lee mas ...
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Video language: English. Attila Kardos has shown up for his photo shoot with the world-famous fashion photographer; Ricky Piedra. During the shoot Attila decides that the swimsuit needs to be filled out just a little more and begins stroking himself and consequently more than adequately fills the swimsuit. Ricky is impressed with Attilas initiative and moves in to inspect how well his bulge has grown. In an instant that bulge is bouncing out of the swimsuit and into Rickys hungry mouth. Ricky masterfully works his way up and down Attilas long cock shaft, making the full length down his throat. Attila can see that Ricky is bursting at the seams and helps to relieve him of his confining clothing. When Rickys cock bounds forward, Attila is there to take it into his mouth. Attila gives Rickys cock a good sucking before he plants his smooth ass on his face. Ricky loves the smooth, sweet taste of Attilas ass and Attila loves the feel of Rickys beard bristling across his ass. Ricky is flipped ...
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Inter and intra species variation in the biofilm production of 54 Trichosporon spp. clinical isolates and 7 reference strains.A- 19 isolates from blood identifi
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