Dive into the research topics of A rapid dot-blot assay to assess chimerism following sex-mismatched bone marrow transplantation. Together they form a unique fingerprint. ...
TY - JOUR. T1 - Donor bone marrow infusions are tolerogenic in human renal transplantation. AU - Ciancio, G.. AU - Garcia-Morales, R.. AU - Mathew, J.. AU - Carreno, M.. AU - Burke, G. W.. AU - Ricordi, C.. AU - Kenyon, N.. AU - Esquenazi, V.. AU - Cirocco, R.. AU - Tzakis, A.. AU - Miller, J.. PY - 2001/1/1. Y1 - 2001/1/1. UR - http://www.scopus.com/inward/record.url?scp=0035087150&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0035087150&partnerID=8YFLogxK. U2 - 10.1016/S0041-1345(00)02485-4. DO - 10.1016/S0041-1345(00)02485-4. M3 - Article. C2 - 11267299. AN - SCOPUS:0035087150. VL - 33. SP - 1295. EP - 1296. JO - Transplantation Proceedings. JF - Transplantation Proceedings. SN - 0041-1345. IS - 1-2. ER - ...
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TY - JOUR. T1 - Pulmonary embolism with bone fragments following vertebral body marrow infusion for tolerance induction. AU - Fernandez, Luis A.. AU - Romaguera, Rita. AU - Viciana, Ana L.. AU - Ruiz, Phillip. AU - Tzakis, Andreas G.. AU - Ricordi, Camillo. PY - 1996. Y1 - 1996. N2 - Protocols of donor bone marrow infusion for tolerance induction are receiving increasing attention in clinical trials of organ allotransplantation. We report pulmonary embolism with bone fragments following vertebral body marrow infusion in a recipient of a liver and intestinal transplant. Even though pulmonary embolism with bony microfragments has been widely described following bone marrow transplantation, the use of single, high-dose donor bone marrow infusion and/or multiple infusions currently under clinical investigation for induction of donor specific unresponsiveness, may warrant the implementation of additional steps in the vertebral body marrow processing technique to decrease or eliminate the component of ...
Chimerism analysis is one part of the monitoring process for bone marrow transplant patients. The pre-transplant chimerism analysis of both donor and recipient is necessary in order to evaluate for the presence of hematopoietic microchimerism in patients at various time points subsequent to allogeneic bone marrow transplant. Hematopoietic microchimerism will be determined by PCR analysis using a panel of highly-polymorphic short tandem repeat (STR) markers. DNA will be extracted from the recipients peripheral blood cells or bone marrow. The number of alleles in the profile established by the STR panel at the time of the pre-transplant chimerism analysis will determine the complexity of the post-transplant chimerism analysis. Screen-informative STRs from post-transplant recipient samples will be quantified to determine the percent of hematopoietic microchimerism in the patient.. ...
The term chimerism in the setting of hematopoietic stem cell transplantation refers to the engraftment or presence of lymphohematopoietic cells of donor origin, typically detected by using techniques of DNA analysis (18). Mixed chimerism, in contrast to full donor chimerism, indicates the presence of both donor and recipient lymphohematopoietic cells. Because mixed versus total donor chimerism can now be reliably generated with specific NST regimens and avoided with other NST regimens, investigators must address an important question: is the state of mixed chimerism after transplantation desirable?. There are at least 3 theoretical advantages of mixed chimerism over full chimerism as an approach to transplantation tolerance (19), as follows: 1) mixed chimerism can be achieved with nonmyeloablative regimens that are less toxic, 2) mixed chimeras have better immune function, and 3) thymic deletion of host-reactive cells occurs to a greater degree in mixed chimeras due to the intrathymic presence ...
Intestinal transplantation is being increasingly performed to treat patients with irreversible intestinal failure. The major cause of intestinal graft failure is graft-versus-host disease (GVHD) that represents a life-threatening complication after small bowel transplantation (Itx). The purpose of this study was to assess the diagnostic and prognostic value of skin biopsy histological changes for acute GVHD after Itx in pigs. Thirty-four Large White pigs were divided into three groups: Group I with Itx only, Group 2 with Itx and donor bone marrow infusion (Itx BM) and Group 3 (control group-before the operation). Animals received tacrolimus-based immunosuppression from day 0 to day 30 postoperatively. Skin and small bowel biopsies were histologically assessed, analysed and classified from grade I to 4 on postoperative days 15, 30, 45 and 60. There was a strong correlation between the histological grading values of skin biopsy changes and the histological grading values of small bowel biopsy ...
Allogeneic bone marrow (BM) engraftment for chimerism and transplantation tolerance may be promoted by combinations of costimulation blocking biologics and small molecular weight inhibitors. We showed previously in a mouse model that anti-CD40Ligand (anti-CD40L, CD154) combined with anti-LFA-1 or everolimus (40-O-(2-hydroxyethyl)-rapamycin) resulted in stable chimerism in almost all BM recipients, whereas anti-LFA-1 plus everolimus conferred approximately 50% chimerism stability. Here, we investigated whether this lower incidence could be increased with deoxyspergualin (DSG) in place of or in addition to everolimus. However, DSG and everolimus were similarly synergistic with costimulation blockade for stable hematopoietic chimerism. This correlated with allospecific T cell depletion and inhibition of acute but not chronic skin allograft rejection. Different treatments were also compared for their inhibition of alloreactive T cell proliferation in vivo. While anti-CD40L did not impair T cell ...
Target cell isolation ideal for chimerism analysis. Positive selection with MACS® MicroBead Technology for multiple cell types from whole blood in 30 min. - 대한민국
TY - JOUR. T1 - Promotion of xenogeneic hematopoietic chimerism in rodents by mononuclear phagocyte depletion. AU - Cheng, J.. AU - Glaser, R. M.. AU - Kruger-Grey, H.. AU - White-Scharf, M. E.. AU - Cooper, D. K.C.. AU - Thall, A. D.. PY - 2002/11. Y1 - 2002/11. N2 - The successful establishment of tolerance toward pig tissues in primates through hematopoietic progenitor cell engraftment is restricted by the rapid disappearance of these cells in the recipient following infusion. We developed and tested the hypothesis that phagocytes of the reticuloendothelial system are responsible for the rapid clearance of infused pig hematopoietic cells using a mouse model. Mice received non-myeloablative conditioning and, on various days, were injected with medronate-encapsulated liposomes (M-L) or control blank liposomes, followed by the intravenous infusion of miniature swine hematopoietic cells. M-L were well-tolerated in mice (n = 100) at levels that deplete mononuclear phagocytes. Depletion of ...
The invention relates to the methods for producing hematopoietic chimerism and central tolerance by peripheral tolerance induction without myeloablative conditioning.
In a murine model, nonmyeloablative conditioning followed by bone marrow transplantation (BMT) led to mixed chimerism and subsequent tolerance to an allogeneic skin graft (19). Mixed chimerism implies stable coexistence of donor and recipient lymphohematopoietic cells in a transplant recipient. Translating this approach to NHP kidney transplantation required successive modifications of the conditioning regimen, finally including total body irradiation, thymic irradiation, and ATG, followed by combined BMT and kidney transplantation, splenectomy, and short-term cyclosporine immunosuppressive treatment (20). Most (9 of 13) NHP transplant recipients had long-term normal renal function without maintenance immunosuppression despite losing chimerism within months of BMT, indicating that long-term tolerance relied upon peripheral rather than central tolerance mechanisms. The same regimen did not achieve tolerance of cardiac allografts (21), suggesting that organ-specific differences influence the ...
Ariffin, H.; Daud, S.S.; Mohamed, Z.; Ibrahim, K.; Lee, T.F.; Chong, L.A. (2007) Evaluation of two short tandem repeat multiplex systems for post-haematopoietic stem cell transplantation chimerism analysis. Singapore Medical Journal, 48 (4). pp. 333-340. ISSN 0037-5675. ...
We analyzed the clinical course and risk factors of 18 patients with poor engraftment after allogeneic bone marrow transplantation (BMT), defined as absolute neutrophil count below 0.1×10 9/1 28 days post-BMT. Significant risks associated with non-engraftment included HLA one antigen mismatch, BMT from matched unrelated donor, and a low dose of colony-forming units-granulocyte-macrophage (,10 4/kg). Examined by a semi-quantitative analysis of polymorphic microsatellite markers, donor DNA chimerism on day 28 was found to be predictive of treatment outcome. Seven patients had detectable donor DNA, varying from 43 to 100%. Five of them responded to granulocyte colony-stimulating factor (G-CSF) and achieved engraftment. Two were given further infusions of peripheral blood hematopoietic stem cells (PBSC) from the same donors, resulting in engraftment in one of them. Eleven patients had no detectable donor DNA, and none responded to G-CSF. Autologous regeneration occurred in six of these patients, ...
The mechanism by which mixed chimerism reverses autoimmunity in type 1 diabetes has not been defined. NOD mice have a well-characterized defect in the production of myeloid progenitors that is believed to contribute significantly to the autoimmune process. We therefore investigated whether chimerism induces a correction of this defect. Mixed chimerism restored production of myeloid progenitors in NOD mice to normal levels. Notably, NOD bone marrow cells as well as donor bone marrow cells produced the mature myeloid progeny, and the level of donor chimerism was not correlated with the degree of restoration of the defect. Moreover, NOD bone marrow cells cultured with Flt3-ligand developed a heat-stable antigen-positive/Ly6C+ population comprised primarily of mature myeloid dendritic cells, suggesting that the underlying abnormality is not cell intrinsic but rather due to a block in development of mature myeloid progeny, including myeloid dendritic cells. Strikingly, treatment of NOD mice with ...
Successful induction of allograft tolerance has been achieved in nonhuman primates (NHPs) and humans via induction of transient hematopoietic chimerism. Since allograft tolerance was achieved in these recipients without durable chimerism, peripheral mechanisms are postulated to play a major role. Here, we report our studies of T cell immunity in NHP recipients that achieved long-term tolerance versus those that rejected the allograft (AR). All kidney, heart, and lung transplant recipients underwent simultaneous or delayed donor bone marrow transplantation (DBMT) following conditioning with a nonmyeloablative regimen. After DBMT, mixed lymphocyte culture with CFSE consistently revealed donor-specific loss of CD8+ T cell responses in tolerant (TOL) recipients, while marked CD4+ T cell proliferation in response to donor antigens was found to persist. Interestingly, a significant proportion of the proliferated CD4+ cells were FOXP3+ in TOL recipients, but not in AR or naive NHPs. In TOL recipients, ...
Having a donor lymphocyte infusion (DLI) means youll get more cells from your original donor. It aims to push your chimerism levels up towards 100%.
Chimerism genetic testing is used to monitor the success of haemopoietic stem cell transplantation (HSCT) by evaluating the ratio of donor and recipient DNA in the recipients blood or bone marrow.. This guides treatment to help prevent engraftment failure, to maximise graft-versus-tumour effect and to minimise opportunistic infection and graft-versus-host disease.. Flow sorted chimerism analysis of peripheral blood can be used to monitor T-cell, B-cell and myeloid lineage specific engraftment upon request.. ...
The results of a small clinical trial offer a way to make umbilical cord blood transplants for leukemia and lymphoma more effective.
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Our clinical investigations demonstrate, that nonmyeloablative stem cell transplantation (NST) is a novel therapeutic option in patients with high-risk ALL or AML. NST can be administered to patients eligible for allogeneic stem cell transplantation with contraindications against high-dose radio- and chemotherapy (standard SCT). After NST, infections and late onset acute graft-versus-host disease (GvHD) frequently occurred, but transplant-related mortality was low in contrast to standard SCT. Leukemia-free survival and overall survival were similar after NST and standard SCT. A higher relapse rate after NST was balanced by a higher transplant-related mortality after standard SCT. The conditioning regimen itself had no relevant impact on survival. Sequential chimerism analyses of leukocyte subpopulations resulted in early diagnosis of mixed chimerism, which proved to be predictive for later relapses. Stable mixed chimerism was not established in these patients. Adoptive immunotherapy in patients, ...
OUTLINE: Patients receive allogeneic white blood cell infusions once daily for 5-10 infusions.. Patients undergo blood sample collection periodically for correlative laboratory studies. The samples are evaluated by in vitro white cell kill assay before the first infusion, immediately after the first infusion, on day 2, and then immediately after the last infusion to assess in vitro cancer cell killing activity. Chimerism studies are performed before the first infusion, immediately after the first infusion, and then on days 2 and 7. Complete chimerism is assayed by short tandem repeat analysis using PCR. Patients with readily accessible tumor tissue (e.g., cervical or axillary lymph nodes or subcutaneous tumor nodules) may also undergo biopsy during the first week of treatment to demonstrate the presence or absence of tumor infiltrating granulocytes.. After completion of study therapy, patients are followed periodically for 3 months. ...
Guest: Dr. Xunrong Luo, Instructor, Departments of Medicine and Pathology, Duke University School of Medicine; Director of Translation, Duke Transplant Center Topic: Transplantation state of the science, transplantation tolerance, immunosuppression, non-chimeric approach, new technologies in transplantation Xunrong Luo Duke faculty page Transplantation Tolerance through Hematopoietic Chimerism: Progress and Challenges for Clinical Translation article…
Abstract. Abstract 4101Background:. Despite the curative promise of hematopoietic cell transplantation (HCT), many patients with hematologic malignancies rela
To the Editor:. The article entitled Evidence for Cardiomyocyte Repopulation by Extracardiac Progenitors in Transplanted Human Hearts by Laflamme et al,1 published in Circulation Research, confirms our early observations2 that cardiac chimerism occurs after transplantation of a female heart in a male patient. These results are also in agreement with the detection of chimerism in multiple organs after bone marrow transplantation.3 The authors of the study in Circulation Research are perturbed by the low frequency of chimerism in their samples compared with ours2 (as well as in a subsequent recent report3). They attribute this discrepancy to …technical differences… (pages 637 and 638) between the two studies. We agree completely with their assessment.. Given the resolution of the histological sections included in the article in Circulation Research, 1 we are pleasantly surprised that they could identify even this relatively low level of chimerism. Laflamme et al place special emphasis on ...
If Im going to the trouble of cloning myself, I want the clone to be a copy of me! Im imagining what someone might say if they were told that their expensive and ethically dubious personal cloning efforts produced a […]. ...
To investigate the antigen specificity of regulatory T cells capable of preventing transplant rejection, we have developed two different strategies to achieve tolerance to fully mismatched skin grafts in euthymic mice. A combination of nondepleting Abs targeting CD4, CD8, and CD154 (CD40 ligand) induces dominant transplantation tolerance to fully mismatched skin allografts. Such tolerance is antigen-specific, mediated by regulatory T cells, and can be extended through linked suppression to naïve lymphocytes. The same protocol, when combined with allogeneic bone marrow, enables the development of mixed hematopoietic chimerism and deletional tolerance. Although we cannot exclude that some regulatory T cells may persist in chimeric mice, these cells are insufficient to mediate linked suppression. CD4(+)CD25(+) T cells, whether taken from naïve mice or from mice tolerized through either treatment protocol, were always able to prevent rejection of skin grafts by naïve CD4(+) T cells, and did so with no
TY - JOUR. T1 - Acute graft-versus-host disease of the heart. AU - Roberts, Stephen S.. AU - Leeborg, Nicky. AU - Loriaux, Marc. AU - Johnson, F. Leonard. AU - Huang, Meei Li. AU - Stenzel, Peter. AU - Thiede, Christian. AU - Godder, Kamar T.. PY - 2006/10/15. Y1 - 2006/10/15. N2 - Graft-versus-host disease (GVHD) is a frequent cause of morbidity and mortality after bone marrow transplantation. Acute GVHD most commonly involves the skin, gastrointestinal tract, and liver. Involvement of other organ systems is rare and remains controversial. We report a patient with GVHD who suffered a fatal ventricular arrhythmia shortly after bone marrow transplantation. Autopsy of the heart showed lymphocyte infiltration. Investigations for cardiotrophic viruses were negative, and chimerism analysis of the heart showed both donor and recipient DNA. We conclude that the cause of death was possibly graft-versus-host disease of the heart. A review of the literature revealed a total of 14 cases of possible cardiac ...
Donor lymphocyte infusions for patients who relapse after allogeneic stem cell transplantation for chronic myeloid leukaemia.: The understanding of the use of d
Results: In untreated control groups (n=5) animals rejected skin grafts, solid organ grafts and hind limb transplants acutely by POD 14 (± 1 day), POD 9 (± 2 days), and 8 (± 1 day), respectively. The induction regimen extended skin graft survival to 32 ± 8 days (n=5). Additional donor BM augmentation lead to allograft survival of 150 days in 4 of 5 recipients. However, indefinite graft survival of ,150 days was observed in all animals receiving the induction regimen and a VCA. Mixed chimerism analysis showed engraftment of donor BM in groups receiving BM at the time of skin transplantation (6.8% ± 3.1%). In groups receiving a VCA alone or an allograft augmented with donor BM and splenocytes, donor chimerism was detected at 22.51% ± 5.96% and 30.17% ± 8.72%, respectively. Vβ-T cell receptor staining showed decreased expression of Vβ 5.1/5.1 and Vβ 8.1/8.2 in animals receiving the full induction regimen compared to controls indicating a central selection and depletion mechanism for ...
Abstract. Introduction: Early response rates to non-myeloablative therapy are encouraging, however long term remissions remain elusive. Manipulating donor lymp
The present invention relates to the use of a non-lethal preparative regimen for the treatment of a patient with human immunodeficiency virus (HIV) disease. In a clinical trial, an HIV-positive patient was conditioned by non-lethal doses of irradiation and a chemotherapeutic drug prior to receiving donor bone marrow cells from a baboon. While long-term engraftment of donor cells was not observed, the non-lethal preparative conditioning regimen was able to reduce the viral burden and improved the clinical outcome. Such method is useful for treatment of patients with advanced acquired immunodeficiency syndrome (AIDS).
followed by donor lymphocyte infusion (DLI) from HLA -haploidentical donors is a safe procedure that will not cause Graft ... (consolidation) at 1.0 g/m2 for 6 dosages followed by HLA-mismatched DLI after the second consolidation. Biological: DLI HLA-mismatched DLI will be administered Day 9, approximately 24-48 hours .... Clinical Trial last updated 05/03/2016 - 9:59am.. ...
We have reached the point in science when creating Chimera is possible, should we continue down this path or does it spell the end for the Human race ...
Peter Rost, M.D., is a former Pfizer Marketing Vice President providing services as a drug expert witness, pharmaceutical marketing expert witness, pharma marketing expert witness, drug industry expert witness, speaker and writer.
Recipient-derived cells integrate into renal allografts inducing organ-specific microchimerism. Circulating pluripotent progenitor cells with high plasticity for differentiation were suggested as a potential source of allograft chimerism. Whether or not these cells also contribute to tumor formation in renal transplants is unknown. We analyzed six histologically different tumors in renal allografts for the presence of recipient-derived cells. To circumvent dependency on gender mismatch, a polymerase chain reaction assay for highly polymorphic short tandem repeat marker (DNA fingerprinting) in combination with laser microdissection was applied. Pure tumor cell populations were harvested by laser microdissection after immunohistochemical (CD45/CD68) marking of contaminating leukocytes. In cases of gender mismatch (n = 2), results were confirmed by sex chromosome in situ hybridization. Two metanephric adenomas demonstrated microchimerism comprising both donor- and recipient-derived tumor cells. Two ...
Following a bone marrow transplant, patients are monitored closely for evidence of graft rejection or recurrence of the original disease. Bone marrow transplantation creates a donor-recipient cellular chimerism in the patient, which can be quantitively measured through short tandem repeat (STR) analysis of peripheral whole blood to determine the percent chimerism of the sample. Increasing recipient chimerism is an indication of graft rejection or relapse. Software programs designed to analyze forensic mixture samples have the potential to be useful in analyzing post-transplant mixed chimeric samples. Post-transplant samples were analyzed using three mixture deconvolution software programs. The programs were fast, accurate and consistent in determining the mixing proportions of the samples and the three programs gave concordant results.
Simon Collins, HIV i-Base. One of the most interesting and intriguing posters at the conference was a case study presented by Gero Hutter from the Medical University of Berlin.. The patient, a 40-year-old man diagnosed in 1995, experienced a relapse of acute myeloid leukemia (AML) that was first diagnosed in 2006. He underwent allogeneic transplant of peripheral stem cells (alloSCT) with an HLA-matched donor, selected to be homozygous for CCR5-delta 32.. A bone marrow registry search identified 232 individuals who had matched HLA, and PCR identified homozygousity for CCR5-d32. HAART was stopped on the day of the transplant. GvHD prophylaxis followed standard regimens and engraftment was achieved on day 13. Complete chimerism detected by competitive PCR was observed on day 60.. Viral load was measured both by RNA-PCR and proviral DNA-PCR. DNA-PCR was negative from day +68.. Previous attempts to use stem cell transplantation as HIV therapy have failed. Here, the investigators claim to have ...
We investigated the impact of the host environment on the survival, expansion, function, and long-term memory function of differentiated GVH-reactive CD4 and CD8 primed T cells. Transfer of GVH-reactive primed T cells generated in lethally irradiated recipients to quiescent mixed chimeras or secondary irradiated hosts allowed us to determine the impact of inflammation on host alloantigen-primed GVH-reactive T cells in the effector phase of GVH alloresponses. Whereas previous studies (14, 15) investigated the effects of inflammation on transferred T cells primed by in vitro antigenic stimulation, GVH-reactive primed T cells in our study were generated in vivo in primary recipients with irradiation-induced inflammation and GVHD. Transfer of GVH-reactive primed T cells from recipients developing GVHD to quiescent mixed chimeras was associated with decreased ability to increase donor chimerism compared with transfer of naive T cells. Consistent with their decreased ability to convert mixed to full ...
One of the functions of the preparative or conditioning regimen before marrow infusion is to create space for the transplanted autologous or allogeneic donor marrow. Marrow for transplantation can be...
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BOSTON -- Six organ-transplant recipients have remained healthy for up to five years without anti-rejection drugs, said researchers in the U.S. and Australia whose strategy was chimerism.
TY - JOUR. T1 - Mixed Chimerism and Secondary Graft Failure in Allogeneic Hematopoietic Stem Cell Transplantation for Aplastic Anemia. AU - Adult Aplastic Anemia Working Group of the Japanese Society for Hematopoietic Cell Transplantation. AU - Kako, Shinichi. AU - Yamazaki, Hirohito. AU - Ohashi, Kazuteru. AU - Ozawa, Yukiyasu. AU - Ota, Shuichi. AU - Kanda, Yoshinobu. AU - Maeda, Tetsuo. AU - Kato, Jun. AU - Ishiyama, Ken. AU - Matsuoka, Ken ichi. AU - Miyamoto, Toshihiro. AU - Iida, Hiroatsu. AU - Ikegame, Kazuhiro. AU - Fukuda, Takahiro. AU - Ichinohe, Tatsuo. AU - Atsuta, Yoshiko. AU - Mori, Takehiko. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Mixed chimerism (MC) and/or secondary graft failure (SGF) with recipient- or donor-type chimerism is a major obstacle in allogeneic transplantation for aplastic anemia (AA). From a registry database in Japan, patients with AA age ,15 years who underwent a first allogeneic bone marrow or peripheral blood stem cell transplantation between 2000 and 2014 and ...
ContextA minimally toxic nonmyeloablative regimen was developed for allogeneic hematopoietic cell transplantation (HCT) to treat patients with advanced hematolo
Paediatric cancer is a disease where the cells of the body grow in an uncontrolled way. Paediatric cancers are those that primarily occur in children. The most common of these are leukaemias, brain tumours and lymphoma. Almost all cancer cells show genetic changes that can be the cause of the cancer or can indicate disease progression.. Paediatric cancer genetic testing is a vital tool, used to identify the cause of the cancer, the type of cancer, help direct the best course of treatment, and to monitor disease progression and the efficacy of treatment.. Chimerism genetic testing is used to monitor the success of blood stem cell transplantation (like bone marrow transplantation). The test looks at the level of donor bone marrow versus the level of bone marrow from the patient (recipient). VCGS has developed an advanced chimerism test which can detect very small changes in the levels of donor and recipient bone marrow. This is used, with high levels of accuracy, to monitor the health of the ...
Graft-versus-host disease (GVHD) is a major cause of morbidity and mortality in hematopoietic stem cell transplantation (HSCT). Donor T cells are key mediators in pathogenesis, but a contribution from host T cells has not been explored, as conditioning regimens are believed to deplete host T cells. To evaluate a potential role for host T cells in GVHD, the origin of skin and blood T cells was assessed prospectively in patients after HSCT in the absence of GVHD. While blood contained primarily donor-derived T cells, most T cells in the skin were host derived. We next examined patient skin, colon, and blood during acute GVHD. Host T cells were present in all skin and colon acute GVHD specimens studied, yet were largely absent in blood. We observed acute skin GVHD in the presence of 100% host T cells. Analysis demonstrated that a subset of host T cells in peripheral tissues were proliferating (Ki67+) and producing the proinflammatory cytokines IFN-γ and IL-17 in situ. Comparatively, the majority ...
Regardless of the MHC mismatching, all animals displayed high-level chimerism at all time points in lymphoid (50-70%), myeloid (40-80%) and granulocyte (40-80%) lineages. MHC Class II-mismatched chimeras remained tolerant of VCAs, without significant histological or clinical features of rejection at any time-point. In contrast, both MHC Class I-mismatched animals experienced acute rejection crises of the epidermal component of the VCAs following tapering of immunosuppression, with one of these animals mounting recurrent rejection episodes. Histological visualization confirmed that rejection was confined to skin epidermis. In vitro assays in all animals demonstrated donor-specific non-responsiveness at all points, including after rejection crises, suggesting that the epidermal rejection in the MHC class I-mismatched animals was a local effect ...
This is a phase II multi-institutional therapeutic study of a non-myeloablative T cell receptor (TCR) alpha/beta depleted haploidentical transplantation with
The two patients in the Boston study had battled HIV for years. They agreed to stop taking their HIV medications earlier this year to test whether the medicine was holding the infections in check, or whether it was the transplant of healthy donor bone marrow cells each received that was vanquishing signs of the virus in their bodies. Both had received the transplants after chemotherapy and other treatments had failed to stop their Hodgkins lymphoma, a cancer of the blood.. For weeks, Henrichs team carefully tested the patients blood, searching for signs of HIV. In July, with one patient off the HIV medications for seven weeks, and the other patient off for 15, the scientists reported their early, encouraging results: They could find no trace of the virus in their blood cells.. But in August, the scientists detected HIV in one of the patients, who then resumed taking medication. The other remained seemingly HIV-free. Concerned about the ethics of continuing the study, the scientists gave the ...
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Loss of chimerism is one of the major problems after allogeneic stem cell transplantation(SCT). Donor- lymphocyte infusions(DLI) are used as a treatment after taper or stopping immunosuppression. In this study, DLI ...
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ashley.hunt60 Wrote:How about the interspecies chimera some are pushing for? Yes please. But in reality, its kind of strange to me. But strange doesnt mean bad. Quote:Souls - If life starts at conce