Cell Line,cell type,cell line specific transfection reagents,EZ Biosystems is a worldwide provider of transfection and gene expression product and services. Simpler, Faster, and Easier are EZ Biosystems promises to scientists in the life science research community. One of the major focuses of EZ Biosystems has been the research, development, and manufacture of next generation transfection reagents ---- Avalanche Transfection Reagent series by applying combinatorial chemistry, molecular biology, and cell biology expertise. This series includes: Avalanche-Cell Type/Cell Line-specific Transfection Reagent series: The pre-optimized transfection Reagent series that ensures the highest transfection efficiency and viability for over 100 cell types/cell lines. Avalanche-Omni Transfection Reagent: Broadest spectrum in vitro gene and siRNA transfection with exceptional transfection efficiency and viability. Avalanche-in vivo Transfection Reagent: Extremely powerful for gene functional studies and RNA
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... : > Pre-optimized Transfection Reagents - > In Vitro DNA Transfection Reagents > In Vivo Transfection Reagents > Pre-optimized Transfection Reagents > In Vitro siRNA Transfection Reagents > Transfection Accessories > Pre-made Adenovirus > shRNA/miRNA AAV Service > Adenovirus Production Service > shRNA/miRNA Adenovirus Svc > AAV Production Service > Ready-to-package Adenovirus > Pre-made AAVs > Pre-made Lentivirus > Lentivirus Production Service SignaGen Laboratories, DNA/siRNA Transfection Reagents, Custom Adenovirus Production Service, Custom AAV Production Service, Custom Lentivirus Production Service
Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types.With Lipofectamine® RNAiMAX Transfection Reagent you will get: • Superior transfection efficiency requiring lower RNAi concentrations, leading to more effective gene knockdown with minimal nonspecific effects• Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent• Superior transfection efficiencies for miRNA antagonists and mimics• Compatibility with a broad range of cell types, providing the most versatile approach to all of your gene silencing experiments• A simple and rapid protocol for consistent and reproducible resultsHigh knockdown in a wide range of cellsLipofectamine® RNAiMAX Transfection Reagent
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... GenJet In Vitro DNA Transfection Reagent for Rin Related Cells [SL100489-RIN] - Description GenJet DNA In Vitro Transfection Reagent for Rin is pre-optimized znd conditioned for transfecting Rin and related cells including Rin-14B, Rin-m5F, Rin-m and Rin-5F. Refer to the following optimal transfection conditions for maximal transfection efficiency on Rin related cells. GenJet reagent, 1.0 ml, is sufficient for 300 to
Altogen Biosystems provides in vivo transfection reagents, over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Transfection reagents are highly efficient for DNA and siRNA transfection in vivo and in vitro. Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell banking cryopreservation services
The choice of a transfection reagent often depends more upon the particular cell line than the substance being delivered into the cells. We have available four different lipid-based transfection reagents that have been specially formulated for delivery of siRNAs into cells. These DharmaFECT reagents are very mild yet have very high transfection rates into a wide variety of cell lines. You can find a list of cells weve tested with DharmaFECT here: http: //dharmacon. horizondiscovery. com/transfection/dharmafect-cell-type-guide/Another recommendation is to use a reagent that has been previously used in the cells of interest. Further optimization for siRNA delivery may be necessary. If no established protocol for the cells is available, a PubMed or HighWire search may help identify if a published protocol for the specific cell line or a similar cell line has been reported. In all instances, we recommend following the protocol provided by the manufacturer of the transfection reagent ...
Low transfection efficiency and low cell viability are the most frequent causes of unsuccessful gene silencing experiments. Through careful optimization--e.g. choosing the right transfection agent and transfection method--high levels of transfection efficiency can be achieved ,Optimizing,siRNA,Transfection,for,RNAi,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
TY - JOUR. T1 - Protective immunity induced by B7.1+IL-12 transfected tumor cells is abrogated by tumor growth in an immune privileged site. AU - Chen, P. W.. AU - Uno, T.. AU - Geer, D. G.. AU - Podack, E. R.. AU - Ksander, B. R.. PY - 1996/12/1. Y1 - 1996/12/1. N2 - Mice immunized with B7.1+IL-12 transfected tumor cells possess systemic anti-tumor immunity that protects mice from a second subcutaneous tumor challenge of untransfected cells. This study determines if these immunized mice are protected from a second tumor challenge delivered to an immunologically privileged site. Our previous results indicated that tumors growing within the immunologically privileged anterior chamber of the eye (AC) of naive mice induce systemic T cell tolerance. In these experiments we determined if tolerance could be imposed after mice were previously sensitized. DBA/2 mice were given a subcutaneous immunization with 1 × 106 P815 B7.1+IL-12 cells. One month later, mice received a second tumor challenge of ...
Altogen Biosystems provides in vivo transfection reagents, over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Transfection reagents are highly efficient for DNA and siRNA transfection in vivo and in vitro. Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell banking cryopreservation services
Images: Ret receptor knockdown using small interference RNA (siRNA) in podocytes. (A) Transfection efficiency: mouse podocytes were transfected with 100 nM concentrations of Ret siRNA or vehicle alone. (a) When podocytes were exposed to i-Fect alone, there was no toxicity. (a and b) A transfection efficiency of nearly 100% was achieved with 100 nM concentration of Ret siRNA (b). Co-transfection with a fluorescently tagged control siRNA was used to determine the transfection efficiency, and fluorescence microscopy revealed a perinuclear localization of the tagged RNA (b, arrowhead). (B) Western blot analysis of Ret after transfection: Ret immunoblotting (top) of WCL 2, 3, or 4 d after transfection revealed that Ret was downregulated within 2 d after transfection with 100 nM Ret siRNA. Transfection of control siRNA at day 4 served as a negative control, and the maximal knockdown of Ret was observed 4 d after transfection. GAPDH immunoblotting confirmed equal protein loading (bottom). ...
Roots Analysis has announced the addition of Non-Viral Transfection Reagents and Systems Market, 2020-2030 report to its list of offerings.. Over time, innovative approaches surrounding the development of potential therapies using non-viral transfection systems have prompted several companies to commercialize proprietary technologies to facilitate gene transfer into cells, via a variety of physical, chemical and other non-viral methods. The growing demand for safe and effective genetically engineered ATMPs is likely to further propel the opportunity for non-viral transfection system developers.. To order this 220+ page report, which features 100+ figures and 125+ tables, please visit the website. Key Market Insights. More than 110 companies claim to offer different types of non-viral transfection systems. The majority of players engaged in the development and commercialization of non-viral transfection systems offer reagents (52%), followed by companies offering electroporation-based ...
G-FECT™ DNA Transfection reagents are a series of powerful DNA transfection reagents with high efficiency DNA delivery and low cell toxicity into mammalian cells. G-FECT™ can be used for plasmid transfection, genome editing, virus production and more.
Forward transfection is a widely used method that works well for adherent cell types; however, if one is using suspension cells or a high-throughput format, reverse transfection, where cells are added to the plated transfection reagent complex, is a more appropriate approach. This method can be used to increase the throughput and reproducibility of a CRISPR-Cas9 screen by optimizing gene editing efficiency as demonstrated in our recently published application note: Optimization of reverse transfection of Dharmacon Edit-R synthetic crRNA and tracrRNA components with DharmaFECT transfection reagent in a Cas9-expressing cell line.. An un-cleavable ubiquitin moiety fused to EGFP allows constitutive degradation of the EGFP protein, while disruption of the proteasome components by functional protein knockout leads to accumulation of EGFP and detectable fluorescence. This functional knockout data shows that determining optimal transfection conditions prior to arrayed screening leads to high gene ...
siPORT NeoFX Transfection Agent 1 ml from Ambion,Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. siPORT NeoFXs lipid-based formulation can be used to efficiently transfect adherent cells as they are,biological,biology supply,biology supplies,biology product
Best transfection method/reagent for HeLa cells? - posted in Cell Biology: Im planning to do some transient transfection of HeLa cells. Aim is to do some CoIP and localization studies. I asked different fellows, some say Fugene is the best, some proposed TFX while other said, this is all crap, use Lipofectamine. Now im a little bit puzzled, which one i should buy... Which transfection method / reagent would you recommend?
Objectives and Background Insulin secretion entirely depends upon Ca2+ influx and sequestration into endoplasmic reticulum (ER) of In diabetes, SERCA2b is decreased within the gene transfected AMSCs for the pancreas of induced diabetes type 1 in rat. injected as with group III. Organizations I, II, IV and III were sacrified 3 weeks following verification of diabetes. Serological, histological, morphometric research and quantitative polymerase string reaction (qPCR) had been performed. Nuclear, cytoplasmic degenerative and intensive fibrotic changes had been detected within the islets of group II that regressed in organizations III and IV. Isolated islet calcium mineral, blood glucose, plasma insulin and qPCR had been confirmative. Conclusions AMSCs and gene transfected AMSCs therapy proved definite therapeutic effect, more obvious in response to gene transfected AMSCs. gene transfected AMSCs on the pancreas of streptozotocin (STZ)-induced diabetes type 1 in adult male albino rat and comparing it ...
Lipofectamine 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene exp
Lipofectamine 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene exp
GeneJuice® Transfection Reagent Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammallian cells. - Find MSDS or SDS, a COA, data sheets and more information.
Lipo2000 Transfection Reagent Lipo 2000 Transfection Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic cells. DNA lipofectamine 2000 complexes must be made in serum-free medium.
Ive done this, its no problem at all! (cells Ive done this on: U87 already transfected with 2 extra (selected) proteins and Hela cells already transfected with 2 proteins and 2 other sequences, thus a total of 4 different pieces of separate DNA integrated stably ...
TransIT®-Keratinocyte from Mirus Bio is a high efficiency, low toxicity, plasmid DNA transfection reagent, specifically optimised for keratinocyte transfection. Ideal for cells including NIKS (near-diploid immortalised keratinocytes) and primary keratinocytes, TransIT-Keratinocyte is suitable for both stable and transient transfections.. Benefits of using TransIT-Keratinocyte ...
Yes, antibiotics can be used during transfection with any of the Life Technologies lipid based reagents. However, the critical steps of the protocol need to be performed with the recommended OptiMEM Media and not culture media that contains any serum or antibiotics. The transfection protocol involves a two tube protocol and It is important to add the lipid reagent to OptiMEM in one tube and the DNA or RNA to OptiMEM in another tube and mix each tube thoroughly. Then, equal amounts from each tube should be mixed to form the transfection complex and incubated for five minutes at room temperature. The lipid-DNA or lipid-RNA complex can then be added directly to cells in complete culture media. Analysis of transfection results can be performed 24-48 hours post transfection. ...
InvivoGen provides LyoVec, a lyophilized cationic lipid-based transfection reagent made of phosphonolipid DTCPTA coupled with DiPPE, a neutral lipid that helps destabilizing membrane bilayers, therefore increasing the in vitro transfection efficiency of LyoVec.
The global transfection reagent & equipment market is expected to reach USD 1,086.02 million by 2022, according to a new report by Grand View Research, Inc. This anticipated growth in demand can be attributed to growing need for protein production, biopharmaceutical development, and vaccine research and development; all of which rely heavily on cytological R&D and transfection.. Complete Report Available @ http://www.radiantinsights.com/research/transfection-reagent-amp-equipment-market. Larger market entities are also involved in efforts to expand their market presence in the Asia Pacific region and tap the high potential for growth available. Expected growth of genomic and proteomic research is also a strong factor which will positively impact the growth in demand for transfection.. Further key findings from the study suggest:. ...
* This reagent has two solutions: Solution A - 0.5 ml, Solution B - 1.0 ml The TransPass™ R2 Transfection Reagent is a two component non-liposomal reagent combination developed specifically for efficient transfection of siRNA in a variety of mammalian cell lines that include “difficult to transfect cells such as primary cells
The goal of stable, long-term transfection is to isolate and propagate individual clones containing transfected DNA that has integrated into the cellular genome. Distinguishing nontransfected cells from those that have taken up exogenous DNA involves selective screening. This screening can be accomplished by drug selection when an appropriate drug-resistance marker is included in the transfected DNA. Alternatively, morphological transformation can be used as a selectable trait in certain cases. For example, bovine papilloma virus vectors produce a morphological change in transfected mouse CI127 cells.. Before using a particular drug for selection purposes, you will need to determine the amount of drug necessary to kill untransfected cells. This may vary greatly among cell types. Consult Current Protocols in Molecular Biology for additional information about designing experiments to test various drug concentrations and determine the amount needed to select resistant clones (i.e., generate a kill ...
줄기세포를 이용한 치료법은 광범위한 세포, 조직, 심지어 장기의 치료에까지 적용될 수 있다. 지방유래 줄기 세포는 다량 추출이 가능하다는 점과 치료 적용시 자가지방을 이용하게 됨으로써 면역반응을 줄일 수 있고, 다양한 세포로 분화가 가능하다. 하지만 충분한 양을 획득하기 위해 생체 밖에서 계대배양을 해야하는데, 이를 지속할 경우 줄기세포의 노화로 인해 줄기세포능을 잃는 어려움이 있다. 이러한 어려움을 개선시키기 위한 방안으로, 세포노화에 관여하는 것으로 알려진 Akt를 저해하는 PTEN 유전자를 선정하였다. PTEN 유전자를 지방 유래 성체줄기세포에 유전자치료는 지속적인 단백질 방출이 가능하기 때문에 한 번의 투여로 지속적인 치료효과를 얻을 수 있다는 장점이 있다. 줄기세포의 노화를 방지하면서 이러한 효과를 지속적으로 유지시켜줄 ...
Sigmas Universal Transfection Reagent is a unique formulation of a proprietary polymer blend used for transient and stable transfection of nucleic acids into various eukaryotic cell lines and hard-to-transfect primary cells. Fast and easy protocol is compatible with serum, serum-free medium and antibiotics
ATCC® Transfection reagents may be used for the transfer of nucleic acids for a variety of applications, including protein expression and the overexpression of mutant genes. Our transfection reagents are cationic lipid-based; due to their positive charge, these transfection reagents interact with the negatively charged backbone of nucleic acids and cell membranes. This effect, coupled with the hydrophobic nature of the associated lipids, allows the cationic lipid-DNA complex to be transported into eukaryotic cells. ATCC offers:. ...
Our i-FectTM Transfection Kit is used to study Epigenetics and pain. Heres yet another example: M. Leinders, b, N. Üçeyler, R.A. Pritchard, C. Sommer, L.S. Sorkin. Increased miR-132-3p expression is associated with chronic neuropathic pain. Experimental Neurology. Volume 283, Part A, September 2016, Pages 276-286...The inhibitor and mimetic were administered to awake rats via the it catheters. Prior to injection, active or mismatch inhibitors were mixed with (1:5 w/v) i-Fect™ in vivo transfection reagent(Neuromics, Edina, USA) to final doses of 5, 2 and 1 μg in 10 μl ...
Looking for a powerful and versatile DNA and siRNA transfection reagent? Try jetPRIME to obtain efficient and reliable scientific results! Request your jetPRIME sample immediately!
GeneX Plus Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells.
Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 μJ/cm2, have found an average transfection efficiency of 50 ± 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.. ©2006 Optical Society of America. Full Article , PDF Article ...
Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell...
METAFECTENE EASY + Fast, easy and effective transfection reagent for mammalian cells For ordering information, MSDS, publications and application notes see Description Cat. No. Size METAFECTENE
Dear flow cytometry people, A problem with the viability of sorted cells was detected in our lab. We have been asked to sort a fibroblast cell line, IOT 1/2. Cells have been transfected using fugene, and GFP expressing cells (about 15%)and non-expressing cells were sorted into 15-ml falcon tubes filled with medium (5 ml) and maintained in ice. Cells before sorting had a clear FS/SS pattern, and viability using PI was about 95-98%. The instrument used was a MoFlo; laser is a Coherent Enterprise operated at 150 mW at 488nm. In the first attempts, a 90-micron tip at 20 PSI was used. A commercial PBS from DakoCytomation was used as sheath fluid. The problem was detected with sorted cells: both positive and negative tubes showed few cells with a low FS signal when reanalysed, and viability using PI was lower than 15-20%. Then we changed to a 200-micron tip at 5 PSI, but number and viability of sorted cells remained very low. Non-transfected cells showed the same behaviour when sorted. I wonder if it ...
Gentaur molecular products has all kinds of products like :search , Neuromi \ n-Blast Transfection Reagent, .75 ml. \ NB30075 for more molecular products just contact us
Gentaur molecular products has all kinds of products like :search , Neuromi \ DNA-Fect, Transfection Reagent, 1 ml. \ DF37100-1 for more molecular products just contact us
Transfection Reagent and Equipment Market Trend, Development Analysis 2020 - Top Countries Data Analysis by Industry Share, Future Prospects, Industry Scope, Opportunity, Boosting Strategies, COVID-19 Impact by ...
Transfection Reagents at Biomol ► Life Science Shop for Research Scientists and Procurement Managers ► For Universities, Research Institutes, and Biotech Companies.
AMSBIO offers transfection reagents including GeneSilencer® with a unique cationic lipid formulation for efficient transfection of siRNA into mammalian cells.
Optimize transfection efficiencies for Cell Line Development; simple cell counting and viability measurements; and total cell counts for beverage contamination quality control.
Synthego is a leading provider of genome engineering solutions. Our flagship product, CRISPRevolution, is a portfolio of synthetic guide RNA designed for CRISPR genome editing and research. Synthegos vision is to bring precision and automation to genome engineering, enabling rapid and cost-effective research with consistent results for every scientist.. ...
FectoPRO®: Improving transient CHO and HEK-293 Expression systems with a powerful transfection solution for high protein production yields.. The development process for producing a biotherapeutic protein usually begins with generating a high-performing stable cell line which can be used for manufacturing. As this step takes a lot of time, transient transfection offers a great alternative to quickly produce milligram to gram quantities of recombinant proteins and antibodies. A various number culture medium are available for performing transient protein production in both CHO and HEK cells but the limiting factor is often the transfection reagent. Thats why we have developed a novel technologically advanced transfection solution called FectoPRO®. Here we show that FectoPRO® outperforms currently available PEI-based and lipid-based transfection reagents in all the transient expression systems tested, offering great transfection efficiency and amazing protein yields.. ...
To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have focused on two transfection vehicles: polyethylenimine (PEI) and calcium phosphate (CaPi). Both have been shown to result in high transfection efficiencies at scales beyond 10 L. Unfortunately, both approaches yield higher levels of recombinant protein (r-protein) in the presence of serum than in its absence. Since serum is a major cost factor and an obstacle to protein purification, our goal was to develop a large-scale TGE process for Chinese hamster ovary (CHO) cells in the absence of serum. CHO-DG44 cells were cultivated and transfected in a chemically defined medium using linear 25 kDa PEI as a transfection vehicle. Parameters that were optimized included the DNA amount, the DNA-to-PEI ratio, the timing and solution conditions for complex formation, the transfection medium, and the cell density at the time of transfection. The highest levels of r-protein expression were observed when cultures ...
TY - JOUR. T1 - Production of HIV virus-like particles by transient transfection of CAP-T cells at bioreactor scale avoiding medium replacement. AU - Gutiérrez-Granados, Sonia. AU - Farràs, Queralt. AU - Hein, Kerstin. AU - Fuenmayor, Javier. AU - Félez, Pablo. AU - Segura, Mercedes. AU - Gòdia, Francesc. PY - 2017/12/10. Y1 - 2017/12/10. N2 - © 2017 Elsevier B.V. Human-derived CAP-T cell line has been demonstrated to be a powerful platform for high-titer production of HIV virus-like particles (VLPs) by PEI-mediated transient transfection. Scale-up of transfection processes is key to ensure the necessary quantities for pre-clinical and clinical testing. One of the major operational challenges of large-scale transient transfection is the medium replacement step that is often required before transfection. In this work, CAP-T cells were cultured in 1 L bioreactor with addition of sodium bicarbonate and surface aeration, which were observed to improve cell state for transfection. Remarkably, ...
In this paper, Beckman Coulter Life Sciences how can the transfection efficiency be improved by using the Biomek i7 Automated Workstation.
The TGEX™-eGFP vector is a green fluorescent reporter designed to monitor transfection efficiency during transient gene expression in mammalian cell suspensions culture. TGEX™-eGFP vector can be used as a single component during transfection to analyze transfection efficiency or at a lower level, between 5% and 10% of the total DNA, to measure efficiency during transient gene expression.. ...
(EMAILWIRE.COM, May 22, 2017 ) Browse 134 market data tables and 43 figures spread through 209 pages and in-depth TOC on Transfection Reagents and Equipment Market http://www.marketsandmarkets.com/Market-Reports/transfection-reagents-equipment-market-139141146.html Early buyers will receive 10%...
ExoFectin® Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome) is a unique blend of polymers designed for the delivery of plasmid DNA into exosomes. This transfection reagent provides highly efficient transfection of plasmid DNA into exosomes, allowing modified exosomes to carry and deliver plasmid DNA into target cells. This kit provides the following advantages:. ...
TY - JOUR. T1 - PEGylation improves the receptor-mediated transfection efficiency of peptide-targeted, self-assembling, anionic nanocomplexes. AU - Tagalakis, Aristides. AU - Kenny, G. AU - Bienemann, A.S.. AU - McCarthy, D. AU - Munye, M.M.. AU - Taylor, H. AU - Wyatt, M.J.. AU - Lyhtgoe, M.F.. AU - White, E.A.. AU - Hart, S.L.. PY - 2014/1/28. Y1 - 2014/1/28. N2 - Non-viral vector formulations comprise typically complexes of nucleic acids with cationic polymers or lipids. However, for in vivo applications cationic formulations suffer from problems of poor tissue penetration, non-specific binding to cells, interaction with serum proteins and cell adhesion molecules and can lead to inflammatory responses. Anionic formulations may provide a solution to these problems but they have not been developed to the same extent as cationic formulations due to difficulties of nucleic acid packaging and poor transfection efficiency. We have developed novel PEGylated, anionic nanocomplexes containing cationic ...
ViaFect Transfection Reagent is a high-performing, low-toxicity reagent designed to efficiently transfect DNA into a wide variety of cell lines.
In 2015, the reagents segment is expected to account for the largest share of the transfection reagents and equipment market, by product; the biochemical method segment is expected to account for the largest share of the global transfection reagents and equipment market, by method; while the biomedical research segment is expected to account for the largest share of the transfection reagents and equipment market, by application.. In 2015, North America is expected to account for the largest share of the global transfection reagents and equipment market, followed by Europe, Asia-Pacific, and rest of the world (RoW). In future, the transfection reagents and equipment market is expected to witness the highest growth rate in the Asia-Pacific region, with emphasis on India, China, and Japan. High growth in these countries can be attributed to the increase in research activities conducted in these regions, rapid expansion of the biotechnology and pharmaceutical industry, government as well as private ...
MACSfectin™ Reagent is applicable for plasmid DNA, mRNA, and siRNA transfection. For enrichment of transfected cells, the MACSelect™ System, which is based on the renowned MACS® Technology, helps to tackle low transfection efficiencies. It is uses the transiently expressed truncated human CD4, the truncated mouse MHC class I H-2Kk, and truncated human LNGFR as surface markers to select transfected cells. Transduction of cells with only low-titer preparations can be achieved by using MACSductin™ Reagent. It consists of polycationic, magnetic beads that help to efficiently transduce primary cells and cell lines using adeno- or retro-/lentiviral vectors ...
Vacic download tristes P technologies are for Differentiating random-coil people. acid of risk into dirged proposals may affect transduced by solitary proteins, doubt research, DEAE-dextran or acetylation. Promega comes download theories spoken on several siblings( TransFast™ Transfection Reagent and ViaFect™ Transfection Reagent), good lessons( FuGENE® 6 invention Reagent and FuGENE® HD Transfection Reagent) and accordance interaction( ProFection® Mammalian Transfection System).
The role of PTEN in cell spreading was also examined in transiently transfected primary human fibroblasts and DBTRG-05MG cells, a glioblastoma cell line with a 204-base pair deletion inPTEN (1). Cells expressing the various constructs were detected by using green fluorescent protein (GFP) as a marker (10). Again, PTEN inhibited cell spreading on FN (Fig. 2, B and C). In human fibroblasts, the effect was maximal at 20 to 30 min, with only 22 ± 8% of PTEN-overexpressing cells spread at 30 min compared with 60 ± 8% of nontransfected cells or cells with control GFP-plasmid (P , 0.001 to 0.005) (Fig. 2C). The majority of cells had spread by 2 hours, however, indicating that PTEN overexpression delayed but did not prevent spreading. Nevertheless, the surface area covered byPTEN-expressing cells remained less than that of controls (see below). In DBTRG-05MG cells, exogenous expression ofPTEN also delayed spreading; even after 18 hours, only 64% of the cells had spread, and the extent of spreading of ...
* found in: Convoy™ Transfection Reagent, Penetratin 1 Peptide, GeneTran™ III Tranfection Reagent, ConvoyTM is new generation cationic polymer gene..
WE ARE A DISTRIBUTING PARTNER OF ScreenFect - InCella ScreenFect® transfection reagents can efficiently transfect a variety of cells with minimal cytoxicit...
Fast SnapFect cell transfection with superior viability and efficiency New way to transfect cells with RNA, DNA, CRISPR Rapid SnapFect Transfection in Flow. (A) The bio-orthogonal mediated nucleic acid transfection strategy is fast and can be performed in a microfluidic format. The mixing of the keto tailored cells wit
Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay.
Middle East and Africa Transfection Reagents and Equipment market size was around USD 28.62 million in 2016. It is expected to grow at a CAGR of 5.6 % to reach USD 37.61 million by 2021. It has the lowest market share of 4%.
Im interested in transient transfection of primary mouse embryo fibroblasts. Does anyone have any experience with this and what are the best methods/protocols for high transfection efficiency (,40%)? Rizwan ...
Plasmid DNA Transfection - http://www.dnatransfection.com/. Information on DNA transfection including plasmid transfection, transient transfection and stable transfection. Includes protocols and articles related to DNA transfection. ...
Transfection is the introduction of nucleic acid molecules into cultured mammalian cells. Chemical transfection reagents provide tools for researchers in a variety of gene research applications.
The MaxCyte® STX™ Scalable Transfection System uses a proprietary flow electroporation technology that can transfect up to 1E10 cells with target, reporter and protein expression plasmids, as well as other molecules, in less than 30 minutes. Transfected cells can be assayed immediately or cryopreserved for future use. Here we demonstrate the use of the MaxCyte STX system in coordination with Cornings ® HYPERFlask® Cell Culture Vessel to provide an efficient and economical solution for culturing large numbers of adherent CHO cells before and after transfection. The HYPERFlask Cell Culture Vessel features Cornings HYPER (High Yield PERformance) technology, which utilizes a gas permeable film to provide gas exchange between the internal culture environment and the external atmospheric environment. The unique, space-saving, 10-layer film design results in 1720 cm2 cell growth surface area, which is approximately 10 times that of a normal T-175 flask. We also show that cells transfected with a ...
Materials. Recombinant neuregulin (rHRGβ1177-244, a peptide of HRGβ1 residues 177-244) was generously provided by Dr. M. Sliwkowski (Holmes et al., 1992). Anti-ERK kinase antibody was a gift from Dr. G. S. Feng. The mouse AChR ε-subunit cDNA was provided by Dr. R. Huganir. The c-fosand c-jun cDNAs were provided by Drs. T. Curran and A. Langley (Curran et al., 1987). Dr. R. Davis provided JNK1, MKK4, and p38 constructs (Whitmarsh et al., 1997), and Dr. M. Birrer provided the c-JUN mammalian expression constructs (Brown et al., 1994). PD98059, AG1478, and SB202190 were purchased from Calbiochem (La Jolla, CA). Rapamycin was from Research Biochemicals (Natick, MA). Cell culture media were purchased from Life Technologies (Gaithersburg, MD). Anti-FLAG antibody and all other chemicals were from Sigma (St. Louis, MO).. Cell culture and transfection procedures. Mouse muscle C2C12 cells were maintained as undifferentiated myoblasts in a nutrient-rich growth medium containing DMEM supplemented with ...
siRNA transfection is a powerful tool used to understand the mechanisms of gene regulation and molecular pathways. The following 10 tips will help you to optimize your siRNA transfection.
Previous studies have implicated Cx-mediated cell-to-cell coupling inβ -cell function (Bosco et al., 1995; Calabrese et al., 2001; Cao et al., 1997; Vozzi et al., 1995) and have suggested that adequate levels of specific Cx isoforms is required for the control of insulin secretion of permanent cell lines (Calabrese et al., 2001; Cao et al., 1997; Vozzi et al., 1995). As yet, however, it has not proved feasible to test this hypothesis directly in normal pancreatic β-cells, mostly because these highly differentiated cells proliferate poorly (Nielsen et al., 1989) and hence are not amenable to the stable expression of exogenous cDNAs by standard transfection procedures. Alternative approaches, which take advantage of viral vectors that can infect pancreatic islets, have been shown to be an effective solution to this problem (Curran et al., 2002; Gallichan et al., 1998; Leibowitz et al., 1999; Salmon et al., 2000). However, these approaches are still limited by the poor viability of β-cells ...
Previous studies have implicated Cx-mediated cell-to-cell coupling inβ -cell function (Bosco et al., 1995; Calabrese et al., 2001; Cao et al., 1997; Vozzi et al., 1995) and have suggested that adequate levels of specific Cx isoforms is required for the control of insulin secretion of permanent cell lines (Calabrese et al., 2001; Cao et al., 1997; Vozzi et al., 1995). As yet, however, it has not proved feasible to test this hypothesis directly in normal pancreatic β-cells, mostly because these highly differentiated cells proliferate poorly (Nielsen et al., 1989) and hence are not amenable to the stable expression of exogenous cDNAs by standard transfection procedures. Alternative approaches, which take advantage of viral vectors that can infect pancreatic islets, have been shown to be an effective solution to this problem (Curran et al., 2002; Gallichan et al., 1998; Leibowitz et al., 1999; Salmon et al., 2000). However, these approaches are still limited by the poor viability of β-cells ...
article{9e981584-ee0b-4f80-867b-a0b6814c78bd, author = {Belting, Mattias and Petersson, Per}, language = {eng}, pages = {281--286}, series = {Biochem. J.}, title = {Protective role for proteoglycans against cationic lipid cytotoxicity allowing optimal transfection efficiency in vitro}, year = {1999 ...
The optimization of the lab intern standard protocols was one of our fields on investigation. Besides optimizing the handling of our different cell lines and working steps, the transfection protocol was examined. One of the most remarkable lessons after several transfection performed was the crucial handling of the AAV293 cells. Once over approximately 80 % confluence the cells are no longer competent for transfection. Another achievement in method development was the determination of the optimal plasmid amounts. The best results were performed using 3.3 µg per plasmid and therefore this parameter was modified in our standard protocol. After transfecting the AAV293 we were able to detect the Ca2+-DNA conglomerates in the medium. The toxic side effects of these conglomerates were also confirmed. Not only the medium had to be changed, but also washing with PBS was essential to keep the cells alive. The most critical steps in transfection is the exact pH of the 2x Hepes buffered-saline (HBS) ...