SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... GenJet In Vitro DNA Transfection Reagent for Rin Related Cells [SL100489-RIN] - Description GenJet DNA In Vitro Transfection Reagent for Rin is pre-optimized znd conditioned for transfecting Rin and related cells including Rin-14B, Rin-m5F, Rin-m and Rin-5F. Refer to the following optimal transfection conditions for maximal transfection efficiency on Rin related cells. GenJet reagent, 1.0 ml, is sufficient for 300 to
Altogen Biosystems provides in vivo transfection reagents, over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Transfection reagents are highly efficient for DNA and siRNA transfection in vivo and in vitro. Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell banking cryopreservation services
The choice of a transfection reagent often depends more upon the particular cell line than the substance being delivered into the cells. We have available four different lipid-based transfection reagents that have been specially formulated for delivery of siRNAs into cells. These DharmaFECT reagents are very mild yet have very high transfection rates into a wide variety of cell lines. You can find a list of cells weve tested with DharmaFECT here: http: //dharmacon. horizondiscovery. com/transfection/dharmafect-cell-type-guide/Another recommendation is to use a reagent that has been previously used in the cells of interest. Further optimization for siRNA delivery may be necessary. If no established protocol for the cells is available, a PubMed or HighWire search may help identify if a published protocol for the specific cell line or a similar cell line has been reported. In all instances, we recommend following the protocol provided by the manufacturer of the transfection reagent ...
Low transfection efficiency and low cell viability are the most frequent causes of unsuccessful gene silencing experiments. Through careful optimization--e.g. choosing the right transfection agent and transfection method--high levels of transfection efficiency can be achieved ,Optimizing,siRNA,Transfection,for,RNAi,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
Altogen Biosystems provides in vivo transfection reagents, over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Transfection reagents are highly efficient for DNA and siRNA transfection in vivo and in vitro. Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell banking cryopreservation services
Images: Ret receptor knockdown using small interference RNA (siRNA) in podocytes. (A) Transfection efficiency: mouse podocytes were transfected with 100 nM concentrations of Ret siRNA or vehicle alone. (a) When podocytes were exposed to i-Fect alone, there was no toxicity. (a and b) A transfection efficiency of nearly 100% was achieved with 100 nM concentration of Ret siRNA (b). Co-transfection with a fluorescently tagged control siRNA was used to determine the transfection efficiency, and fluorescence microscopy revealed a perinuclear localization of the tagged RNA (b, arrowhead). (B) Western blot analysis of Ret after transfection: Ret immunoblotting (top) of WCL 2, 3, or 4 d after transfection revealed that Ret was downregulated within 2 d after transfection with 100 nM Ret siRNA. Transfection of control siRNA at day 4 served as a negative control, and the maximal knockdown of Ret was observed 4 d after transfection. GAPDH immunoblotting confirmed equal protein loading (bottom). ...
G-FECT™ DNA Transfection reagents are a series of powerful DNA transfection reagents with high efficiency DNA delivery and low cell toxicity into mammalian cells. G-FECT™ can be used for plasmid transfection, genome editing, virus production and more.
Forward transfection is a widely used method that works well for adherent cell types; however, if one is using suspension cells or a high-throughput format, reverse transfection, where cells are added to the plated transfection reagent complex, is a more appropriate approach. This method can be used to increase the throughput and reproducibility of a CRISPR-Cas9 screen by optimizing gene editing efficiency as demonstrated in our recently published application note: "Optimization of reverse transfection of Dharmacon Edit-R synthetic crRNA and tracrRNA components with DharmaFECT transfection reagent in a Cas9-expressing cell line.". An un-cleavable ubiquitin moiety fused to EGFP allows constitutive degradation of the EGFP protein, while disruption of the proteasome components by functional protein knockout leads to accumulation of EGFP and detectable fluorescence. This functional knockout data shows that determining optimal transfection conditions prior to arrayed screening leads to high gene ...
siPORT NeoFX Transfection Agent 1 ml from Ambion,Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. siPORT NeoFXs lipid-based formulation can be used to efficiently transfect adherent cells as they are,biological,biology supply,biology supplies,biology product
Best transfection method/reagent for HeLa cells? - posted in Cell Biology: Im planning to do some transient transfection of HeLa cells. Aim is to do some CoIP and localization studies. I asked different fellows, some say Fugene is the best, some proposed TFX while other said, this is all crap, use Lipofectamine. Now im a little bit puzzled, which one i should buy... Which transfection method / reagent would you recommend?
Lipofectamine 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene exp
GeneJuice® Transfection Reagent Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammallian cells. - Find MSDS or SDS, a COA, data sheets and more information.
Ive done this, its no problem at all! (cells Ive done this on: U87 already transfected with 2 extra (selected) proteins and Hela cells already transfected with 2 proteins and 2 other sequences, thus a total of 4 different pieces of separate DNA integrated stably ...
Yes, antibiotics can be used during transfection with any of the Life Technologies lipid based reagents. However, the critical steps of the protocol need to be performed with the recommended OptiMEM Media and not culture media that contains any serum or antibiotics. The transfection protocol involves a two tube protocol and It is important to add the lipid reagent to OptiMEM in one tube and the DNA or RNA to OptiMEM in another tube and mix each tube thoroughly. Then, equal amounts from each tube should be mixed to form the transfection complex and incubated for five minutes at room temperature. The lipid-DNA or lipid-RNA complex can then be added directly to cells in complete culture media. Analysis of transfection results can be performed 24-48 hours post transfection. ...
InvivoGen provides LyoVec, a lyophilized cationic lipid-based transfection reagent made of phosphonolipid DTCPTA coupled with DiPPE, a neutral lipid that helps destabilizing membrane bilayers, therefore increasing the in vitro transfection efficiency of LyoVec.
The global transfection reagent & equipment market is expected to reach USD 1,086.02 million by 2022, according to a new report by Grand View Research, Inc. This anticipated growth in demand can be attributed to growing need for protein production, biopharmaceutical development, and vaccine research and development; all of which rely heavily on cytological R&D and transfection.. Complete Report Available @ http://www.radiantinsights.com/research/transfection-reagent-amp-equipment-market. Larger market entities are also involved in efforts to expand their market presence in the Asia Pacific region and tap the high potential for growth available. Expected growth of genomic and proteomic research is also a strong factor which will positively impact the growth in demand for transfection.. Further key findings from the study suggest:. ...
* This reagent has two solutions: Solution A - 0.5 ml, Solution B - 1.0 ml The TransPass™ R2 Transfection Reagent is a two component non-liposomal reagent combination developed specifically for efficient transfection of siRNA in a variety of mammalian cell lines that include “difficult to transfect cells such as primary cells
줄기세포를 이용한 치료법은 광범위한 세포, 조직, 심지어 장기의 치료에까지 적용될 수 있다. 지방유래 줄기 세포는 다량 추출이 가능하다는 점과 치료 적용시 자가지방을 이용하게 됨으로써 면역반응을 줄일 수 있고, 다양한 세포로 분화가 가능하다. 하지만 충분한 양을 획득하기 위해 생체 밖에서 계대배양을 해야하는데, 이를 지속할 경우 줄기세포의 노화로 인해 줄기세포능을 잃는 어려움이 있다. 이러한 어려움을 개선시키기 위한 방안으로, 세포노화에 관여하는 것으로 알려진 Akt를 저해하는 PTEN 유전자를 선정하였다. PTEN 유전자를 지방 유래 성체줄기세포에 유전자치료는 지속적인 단백질 방출이 가능하기 때문에 한 번의 투여로 지속적인 치료효과를 얻을 수 있다는 장점이 있다. 줄기세포의 노화를 방지하면서 이러한 효과를 지속적으로 유지시켜줄 ...
Sigmas Universal Transfection Reagent is a unique formulation of a proprietary polymer blend used for transient and stable transfection of nucleic acids into various eukaryotic cell lines and hard-to-transfect primary cells. Fast and easy protocol is compatible with serum, serum-free medium and antibiotics
ATCC® Transfection reagents may be used for the transfer of nucleic acids for a variety of applications, including protein expression and the overexpression of mutant genes. Our transfection reagents are cationic lipid-based; due to their positive charge, these transfection reagents interact with the negatively charged backbone of nucleic acids and cell membranes. This effect, coupled with the hydrophobic nature of the associated lipids, allows the cationic lipid-DNA complex to be transported into eukaryotic cells. ATCC offers:. ...
Our i-FectTM Transfection Kit is used to study Epigenetics and pain. Heres yet another example: M. Leinders, b, N. Üçeyler, R.A. Pritchard, C. Sommer, L.S. Sorkin. Increased miR-132-3p expression is associated with chronic neuropathic pain. Experimental Neurology. Volume 283, Part A, September 2016, Pages 276-286...The inhibitor and mimetic were administered to awake rats via the it catheters. Prior to injection, active or mismatch inhibitors were mixed with (1:5 w/v) i-Fect™ in vivo transfection reagent(Neuromics, Edina, USA) to final doses of 5, 2 and 1 μg in 10 μl ...
Looking for a powerful and versatile DNA and siRNA transfection reagent? Try jetPRIME to obtain efficient and reliable scientific results! Request your jetPRIME sample immediately!
GeneX Plus Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells.
Xfect Transfection reagent creates bio-degradable nanoparticles for superior transfection efficiency of plasmid DNA into mammalian cells.
Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 μJ/cm2, have found an average transfection efficiency of 50 ± 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.. ©2006 Optical Society of America. Full Article , PDF Article ...
Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell...
METAFECTENE EASY + Fast, easy and effective transfection reagent for mammalian cells For ordering information, MSDS, publications and application notes see Description Cat. No. Size METAFECTENE
Dear flow cytometry people, A problem with the viability of sorted cells was detected in our lab. We have been asked to sort a fibroblast cell line, IOT 1/2. Cells have been transfected using fugene, and GFP expressing cells (about 15%)and non-expressing cells were sorted into 15-ml falcon tubes filled with medium (5 ml) and maintained in ice. Cells before sorting had a clear FS/SS pattern, and viability using PI was about 95-98%. The instrument used was a MoFlo; laser is a Coherent Enterprise operated at 150 mW at 488nm. In the first attempts, a 90-micron tip at 20 PSI was used. A commercial PBS from DakoCytomation was used as sheath fluid. The problem was detected with sorted cells: both positive and negative tubes showed few cells with a low FS signal when reanalysed, and viability using PI was lower than 15-20%. Then we changed to a 200-micron tip at 5 PSI, but number and viability of sorted cells remained very low. Non-transfected cells showed the same behaviour when sorted. I wonder if it ...
Gentaur molecular products has all kinds of products like :search , Neuromi \ n-Blast Transfection Reagent, .75 ml. \ NB30075 for more molecular products just contact us
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... at Biomol ► Life Science Shop for Research Scientists and Procurement Managers ► For Universities, Research Institutes, and Biotech Companies.
AMSBIO offers transfection reagents including GeneSilencer® with a unique cationic lipid formulation for efficient transfection of siRNA into mammalian cells.
Synthego is a leading provider of genome engineering solutions. Our flagship product, CRISPRevolution, is a portfolio of synthetic guide RNA designed for CRISPR genome editing and research. Synthegos vision is to bring precision and automation to genome engineering, enabling rapid and cost-effective research with consistent results for every scientist.. ...
The MaxCyte STX Scalable Transfection System provides an ideal solution for rapidly expressing a large number of antibodies or proteins in the quantities needed for use in screening and other pre-clinical drug development applications. The MaxCyte STX uses flow electroporation to reproducibly transfect an array of cell types, including CHO cells and other cells commonly used for protein production.
Transfection of primary cells, stem cells and difficult-to-transfect cell lines and their use in cellular screening assays will be featured by MaxCyte. They will also present the use of the MaxCyte® STX™ Scalable Transfection System for large-scale transient transfection.
Hi everyone , , Ive been trying to transfect alpha T-3 cells with a luciferase , construct using the calsium phosphate method of transfection. The DNA I , used for this transfection was isolated with Promegas Wizard , Maxipreps. To date, all my transfections were unsuccessfull. Does , anybody have any ideas why the transfections wont work? , , Thanks , Rentia I have used Promegas Wizard minipreps and maxipreps to prepare plasmid DNA that was suitable for transfection. The cell lines that I transfected were Daudi and Owl Monkey 531H. I transfected by electro- poration. I dont know what might be wrong with your preparations. -- Rainforest laid low. Wake up and smell the ozone, Says man with chainsaw. - John Ladasky ...
Your German Partner for Transfection, Gene Expression, Fluorescent Probes, IVD Kits, Antibodies, Peptides, Lipidomics, Glycobiology, Lab Supplies, and more.
|p|Transfection — the delivery of DNA or RNA into eukaryotic cells — is a powerful tool used to study and control gene expression. Cloned genes can be transfected into cells for biochemical characterization, mutational analyses, investigation of the effects of gene expression on cell growth, investigation of gene regulatory elements, and to produce a specific protein. Transfection of RNA can be used either to induce protein expression, or to repress it using antisense or RNA interference (RNAi) procedures.|/p| |p|There are two types of transfection — transient and stable — suited to different experimental applications, and with different vector requirements.|/p|
Polyplus Transfection Licenses Kempbio For Mammalian Transient Transfection Technology - read this article along with other careers information, tips and advice on BioSpace
The COP9 signalosome is a highly conserved protein complex composed of eight subunits. In this study a novel, regulatory mechanism of CSN biogenesis was identified. We used stable transfected siCSN1 cells in which the protein and the mRNA expression of CSN subuntis were downregulated. Transfection of His-CSN1 in those siCSN1 cells led to the induction of the de novo Synthesis of the whole CSN complex. In addition the expression of the transcription factors STAT1 and c-Myc was elevated. The cells were treated with IFN alpha or IFN gamma, respectively. This resulted in the induction of the CSN de novo synthesis. Moreover, the siRNA-mediated inhibition of STAT1, c-Myc, Lin28B as well as treatment with the pharmacological inhibitors AG9 or AG490 led to a reduced protein expression of the analysed CSN subunits. We found that in all experiments there was a significant change on protein level in contrast to a marginal change on the RNA level. Based on our study we hypothesized that the CSN biogenesis ...
The mouse myoblast C2C12 cell line transfected singly with cDNA for Pax-3, PAX3-FKHR, or insulin-like growth factor (IGF) II or cotransfected with IGF-II plus Pax-3 or with IGF-II plus PAX3-FKHR genes showed an altered morphology, a lack of differentiation, and higher proliferation rates in vitro. On s.c. injection into nude mice, tumors grew from transfected cell lines but not from cells transfected with the empty vector. Tumors derived from IGF-II/PAX3-FKHR- and IGF-II-transfected cells grew most rapidly. Cotransfection of IGF-II plus Pax-3 induced tumors comprised highly differentiated striated muscle cells; Pax-3, PAX3-FKHR, or IGF-II transfection produced tumors at varying stages of differentiation. Tumors derived from IGF-II plus PAX3-FKHR-cotransfected cells were composed of undifferentiated cells. This was the only tumor type to infiltrate the underlying muscle. The most angiogenesis and the least apoptosis were observed in the latter tumors. These results support the hypothesis that ...
Sigma-Aldrich offers abstracts and full-text articles by [Liwen Chen, Shihe Guan, Qiang Zhou, Shouqin Sheng, Fei Zhong, Qin Wang].
Hi Ricky, unless you use very sensititve or special cells (eg. primary) endotoxins dont matter much. Standard cell lines like CHO, HeLa, COS, HEK293, HepG2 etc. trnasfect easily and well with CaPO4 or liposomes using plasmids prepped with normal Qiagen or Promega or other protocols (ie not their endofree variants) . However purity matters generally, as well as degradation (eg. exposing the DNA too long to the first lysis buffer that generally contain NaOH). Another concern is losing a plasmid by recombination or contamination... Bruno Ricky Leung ,b883732 at logic.itsc.cuhk.edu.hk, wrote in message news:9t8hsl$1ml$1 at justice.csc.cuhk.edu.hk... , Hi everyone, , , Ive a problem during transfection. I tried to transfect pcDNA-lacZ to , mammalian cells using lipofectamine plus. When I used stock pcDNA-lacZ for , transfection, everything worked fine and b-gal was obviously detected by , western blot analysis. However, when I used pcDNA-lacZ which was prepared , from Concert miniprep kit, no ...
LASP1 expression was suppressed by miR-218 transfection of prostate cancer (PCa) cells. (a) LASP1 mRNA expression 72 h after transfection with miR-218. GUSB exp
Do you want an optimized Cas9 protein to increase your genome editing efficiency with jetCRISPR RNP transfection reagent? Visit our website to know how this SpCas9 Nuclease!
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For an RNAi or antisense experiment, the actual level of target gene knockdown is related to the transfection efficiency. A positive control such as HPRT DsiRNA should be used in each experiment to assess transfection efficiency. In addition, IDT recommends using a dose-response curve of 0.1, 1, and 10 nM to determine maximum response ...
With the Cellometer Vision CBA, just 20 microliter of sample is added to the Cellometer Counting Chamber. Imaging and analysis of GFP expression is completed in less than 60 seconds.
The primary purpose of this website is to help encourage this by directing viewers to resources that are available for different cell types.
Buy our TLR4 293T transfected lysate (positive control). ab94063 has been validated in western blot. Abcam now offers a 12-month guarantee.
Buy our Dlx1 293T transfected lysate (positive control). ab94117 has been validated in western blot. Abcam now offers a 12-month guarantee.
To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have focused on two transfection vehicles: polyethylenimine (PEI) and calcium phosphate (CaPi). Both have been shown to result in high transfection efficiencies at scales beyond 10 L. Unfortunately, both approaches yield higher levels of recombinant protein (r-protein) in the presence of serum than in its absence. Since serum is a major cost factor and an obstacle to protein purification, our goal was to develop a large-scale TGE process for Chinese hamster ovary (CHO) cells in the absence of serum. CHO-DG44 cells were cultivated and transfected in a chemically defined medium using linear 25 kDa PEI as a transfection vehicle. Parameters that were optimized included the DNA amount, the DNA-to-PEI ratio, the timing and solution conditions for complex formation, the transfection medium, and the cell density at the time of transfection. The highest levels of r-protein expression were observed when cultures ...
Mouse embryonic stem cell transfection with over 90% efficiency and low toxicity. Outperforms other lipid-based transfection reagents.
In this paper, Beckman Coulter Life Sciences how can the transfection efficiency be improved by using the Biomek i7 Automated Workstation.
The TGEX™-eGFP vector is a green fluorescent reporter designed to monitor transfection efficiency during transient gene expression in mammalian cell suspensions culture. TGEX™-eGFP vector can be used as a single component during transfection to analyze transfection efficiency or at a lower level, between 5% and 10% of the total DNA, to measure efficiency during transient gene expression.. ...
(EMAILWIRE.COM, May 22, 2017 ) Browse 134 market data tables and 43 figures spread through 209 pages and in-depth TOC on Transfection Reagents and Equipment Market http://www.marketsandmarkets.com/Market-Reports/transfection-reagents-equipment-market-139141146.html Early buyers will receive 10%...
ExoFectin® Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome) is a unique blend of polymers designed for the delivery of plasmid DNA into exosomes. This transfection reagent provides highly efficient transfection of plasmid DNA into exosomes, allowing modified exosomes to carry and deliver plasmid DNA into target cells. This kit provides the following advantages:. ...
In 2015, the reagents segment is expected to account for the largest share of the transfection reagents and equipment market, by product; the biochemical method segment is expected to account for the largest share of the global transfection reagents and equipment market, by method; while the biomedical research segment is expected to account for the largest share of the transfection reagents and equipment market, by application.. In 2015, North America is expected to account for the largest share of the global transfection reagents and equipment market, followed by Europe, Asia-Pacific, and rest of the world (RoW). In future, the transfection reagents and equipment market is expected to witness the highest growth rate in the Asia-Pacific region, with emphasis on India, China, and Japan. High growth in these countries can be attributed to the increase in research activities conducted in these regions, rapid expansion of the biotechnology and pharmaceutical industry, government as well as private ...
MACSfectin™ Reagent is applicable for plasmid DNA, mRNA, and siRNA transfection. For enrichment of transfected cells, the MACSelect™ System, which is based on the renowned MACS® Technology, helps to tackle low transfection efficiencies. It is uses the transiently expressed truncated human CD4, the truncated mouse MHC class I H-2Kk, and truncated human LNGFR as surface markers to select transfected cells. Transduction of cells with only low-titer preparations can be achieved by using MACSductin™ Reagent. It consists of polycationic, magnetic beads that help to efficiently transduce primary cells and cell lines using adeno- or retro-/lentiviral vectors ...
Vacic download tristes P technologies are for Differentiating random-coil people. acid of risk into dirged proposals may affect transduced by solitary proteins, doubt research, DEAE-dextran or acetylation. Promega comes download theories spoken on several siblings( TransFast™ Transfection Reagent and ViaFect™ Transfection Reagent), good lessons( FuGENE® 6 invention Reagent and FuGENE® HD Transfection Reagent) and accordance interaction( ProFection® Mammalian Transfection System).
The role of PTEN in cell spreading was also examined in transiently transfected primary human fibroblasts and DBTRG-05MG cells, a glioblastoma cell line with a 204-base pair deletion inPTEN (1). Cells expressing the various constructs were detected by using green fluorescent protein (GFP) as a marker (10). Again, PTEN inhibited cell spreading on FN (Fig. 2, B and C). In human fibroblasts, the effect was maximal at 20 to 30 min, with only 22 ± 8% of PTEN-overexpressing cells spread at 30 min compared with 60 ± 8% of nontransfected cells or cells with control GFP-plasmid (P , 0.001 to 0.005) (Fig. 2C). The majority of cells had spread by 2 hours, however, indicating that PTEN overexpression delayed but did not prevent spreading. Nevertheless, the surface area covered byPTEN-expressing cells remained less than that of controls (see below). In DBTRG-05MG cells, exogenous expression ofPTEN also delayed spreading; even after 18 hours, only 64% of the cells had spread, and the extent of spreading of ...
* found in: Convoy™ Transfection Reagent, Penetratin 1 Peptide, GeneTran™ III Tranfection Reagent, ConvoyTM is new generation cationic polymer gene..
WE ARE A DISTRIBUTING PARTNER OF ScreenFect - InCella ScreenFect® transfection reagents can efficiently transfect a variety of cells with minimal cytoxicit...
Fast SnapFect cell transfection with superior viability and efficiency New way to transfect cells with RNA, DNA, CRISPR Rapid SnapFect Transfection in Flow. (A) The bio-orthogonal mediated nucleic acid transfection strategy is fast and can be performed in a microfluidic format. The mixing of the keto tailored cells wit
Middle East and Africa Transfection Reagents and Equipment market size was around USD 28.62 million in 2016. It is expected to grow at a CAGR of 5.6 % to reach USD 37.61 million by 2021. It has the lowest market share of 4%.
Plasmid DNA Transfection - http://www.dnatransfection.com/. Information on DNA transfection including plasmid transfection, transient transfection and stable transfection. Includes protocols and articles related to DNA transfection. ...
The MaxCyte® STX™ Scalable Transfection System uses a proprietary flow electroporation technology that can transfect up to 1E10 cells with target, reporter and protein expression plasmids, as well as other molecules, in less than 30 minutes. Transfected cells can be assayed immediately or cryopreserved for future use. Here we demonstrate the use of the MaxCyte STX system in coordination with Cornings ® HYPERFlask® Cell Culture Vessel to provide an efficient and economical solution for culturing large numbers of adherent CHO cells before and after transfection. The HYPERFlask Cell Culture Vessel features Cornings HYPER (High Yield PERformance) technology, which utilizes a gas permeable film to provide gas exchange between the internal culture environment and the external atmospheric environment. The unique, space-saving, 10-layer film design results in 1720 cm2 cell growth surface area, which is approximately 10 times that of a normal T-175 flask. We also show that cells transfected with a ...
Previous studies have implicated Cx-mediated cell-to-cell coupling inβ -cell function (Bosco et al., 1995; Calabrese et al., 2001; Cao et al., 1997; Vozzi et al., 1995) and have suggested that adequate levels of specific Cx isoforms is required for the control of insulin secretion of permanent cell lines (Calabrese et al., 2001; Cao et al., 1997; Vozzi et al., 1995). As yet, however, it has not proved feasible to test this hypothesis directly in normal pancreatic β-cells, mostly because these highly differentiated cells proliferate poorly (Nielsen et al., 1989) and hence are not amenable to the stable expression of exogenous cDNAs by standard transfection procedures. Alternative approaches, which take advantage of viral vectors that can infect pancreatic islets, have been shown to be an effective solution to this problem (Curran et al., 2002; Gallichan et al., 1998; Leibowitz et al., 1999; Salmon et al., 2000). However, these approaches are still limited by the poor viability of β-cells ...
article{9e981584-ee0b-4f80-867b-a0b6814c78bd, author = {Belting, Mattias and Petersson, Per}, language = {eng}, pages = {281--286}, series = {Biochem. J.}, title = {Protective role for proteoglycans against cationic lipid cytotoxicity allowing optimal transfection efficiency in vitro}, year = {1999 ...
The optimization of the lab intern standard protocols was one of our fields on investigation. Besides optimizing the handling of our different cell lines and working steps, the transfection protocol was examined. One of the most remarkable lessons after several transfection performed was the crucial handling of the AAV293 cells. Once over approximately 80 % confluence the cells are no longer competent for transfection. Another achievement in method development was the determination of the optimal plasmid amounts. The best results were performed using 3.3 µg per plasmid and therefore this parameter was modified in our standard protocol. After transfecting the AAV293 we were able to detect the Ca2+-DNA conglomerates in the medium. The toxic side effects of these conglomerates were also confirmed. Not only the medium had to be changed, but also washing with PBS was essential to keep the cells alive. The most critical steps in transfection is the exact pH of the 2x Hepes buffered-saline (HBS) ...
The optimization of the lab intern standard protocols was one of our fields on investigation. Besides optimizing the handling of our different cell lines and working steps, the transfection protocol was examined. One of the most remarkable lessons after several transfection performed was the crucial handling of the AAV293 cells. Once over approximately 80 % confluence the cells are no longer competent for transfection. Another achievement in method development was the determination of the optimal plasmid amounts. The best results were performed using 3.3 µg per plasmid and therefore this parameter was modified in our standard protocol. After transfecting the AAV293 we were able to detect the Ca2+-DNA conglomerates in the medium. The toxic side effects of these conglomerates were also confirmed. Not only the medium had to be changed, but also washing with PBS was essential to keep the cells alive. The most critical steps in transfection is the exact pH of the 2x Hepes buffered-saline (HBS) ...
MACSfectin™ Reagent enables optimized transfection of plasmid DNA for a wide range of adherent and suspension cells in immunology, cancer, stem cell, and neuroscience research. Additionally, MACSfectin Reagent supports tranfection with RNA such as mRNA or siRNA. This unique transfection reagent is based on a novel class of cationic lipopolyamines designed to optimize nucleic acid condensation and augment the cytoplasmic release of the nucleic acid cargo. Additionally, good transgene expression with high cell viability is achieved due to MACSfectin Reagents excellent biodegradation characteristics. - Belgique
Based on an aqueous formulation of cationic and neutral lipids, FlyFectin has been developed exclusively to transfect insect cells.
Browse the product groups below to find reporter assays, a comprehensive range of reporter vectors, luciferase-expressing cell lines and low-toxicity transfection reagents to ensure successful delivery of vectors to the cell line of your choice.
Principal Investigator:NAKANISHI Mamoru, Project Period (FY):1995 - 1997, Research Category:Grant-in-Aid for Scientific Research (A), Section:展開研究, Research Field:Biophysics
Gymnosis combines specifically modified antisense oligos with a cells normal growth properties to achieve sequence-specific target knockdown without the need for transfection reagents or serum additives, according to its developers.
Transfections were performed in six-well culture plates (Corning Inc., Corning, NY). Each well has approximately 10-cm2 surface for cell culture growth. Cells in each well were transfected with 2 μg of total plasmid DNA. To test the transactivating activity of wild-type and mutant hPXRs, the cells were transfected with 50 ng of pcDNA3, wild-type or mutant hPXR, and 1950 ng of pGL3-CYP3A4-luc plasmids. To examine the protein expression and localization, the cells were transfected with 2 μg of pcDNA3 and wild-type or mutant hPXR plasmids. For the rapamycin experiments, the cells were transfected with 50 ng of pcDNA3 or hPXR and 1950 ng of pGL3-CYP3A4-luc plasmids. In p70 S6K overexpression experiments, the cells were transfected with 100 ng of FLAG-pcDNA3, pcDNA3-FLAG-hPXR, or pcDNA3-FLAG-hPXRT57A; 500 ng of constitutively active p70 S6K; and 1000 ng of pGL3-CYP3A4-luc and 100 ng of pGL4-hRluc; FLAG-pcDNA3 was used to make up the total plasmid DNA to 2 μg in each transfection. In mammalian ...
Your feedback on the specific cell lines and conditions used in your lab will provide other researchers with valuable information. Visit the Transfection Cell Database to view transfection data for a range of cell types and transfection reagents. In appreciation of your feedback, you will receive a free gift.. ...
摘要 本研究旨在克隆从江香猪FoxO 4基因编码区序列,对FoxO 4基因功能进行初步探究。采用qRT-PCR技术对从江香猪(6月龄)不同组织中FoxO 4基因的相对表达量进行检测;PCR扩增FoxO 4基因CDS区,构建真核表达载体pEGFP-N3-FoxO4;利用脂质体法将重组质粒pEGFP-N3-FoxO4瞬时转染从江香猪皮下脂肪前体细胞,通过qRT-PCR方法,检测FoxO 4、PPARγ、LPL、FAS、ACC、ATGL、HSL的表达水平。结果显示:FoxO 4基因在从江香猪脂肪组织中表达量最高;构建的重组真核表达载体pEGFP-N3-FoxO4在从江香猪皮下脂肪前体细胞中成功表达,与对照组相比,LPL、FAS、ACC、ATGL、HSL基因的表达均显著上调。综上表明,FoxO 4对脂质代谢具有一定的调控作用,可能是影响从江香猪猪肉品质的重要候选基因,为进一步了解从江香猪脂肪沉积机制提供理论基础。. ...
(Qin) Infection of the chANXA2-transfected cells.Geese embryo fibroblasts (GEF) and 293T cells were transfected with chANXA2, and the transfected cells were the
Your German Partner for Transfection, Gene Expression, Fluorescent Probes, IVD Kits, Antibodies, Peptides, Lipidomics, Glycobiology, Lab Supplies, and more.
The experimental introduction of genes or gene fragments directly into cells in the form of isolated DNA. Various methods such as electroporation and the use of reagents such as lipofectamine, are used to facilitate the passage of the DNA across the plasma membrane. ...
Lonza offers laboratory equipment for Nucleic Acid Electrophoresis and for Transfection. FlashGel System, Nucleofector 4D Device, Nucleofector 2b Device.
Vergleichen Sie Nucleofection Kits Produkte von renommierten Herstellern bei SZABO-SCANDIC. Sie finden Produktdetails, Preise, Verfügbarkeit und mehr.
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
A fusion reagent for rapid and efficient gene silencing in living cells-the improvement of siRNA transfection Exceptionally efficient siRNA transfection, esp...
Caltag Medsystems Cell Culture section of our portfolio ranges from primary cells, stem cells, bone marrowand media to skin, CTC isolation devices, human and animal tissue, 3D scaffolds,angiogenesis models and transfection reagents.
Video articles in JoVE about dna viral include Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples, Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells, Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation.
Abnova KIAA1530 293T Cell Transient Overexpression Lysate (Denatured) 100µL Life Sciences:Protein Biology:Proteins:Lysates:Overexpression Lysates K
Abnova DCI 293T Cell Transient Overexpression Lysate (Denatured) 100µL Life Sciences:Protein Biology:Proteins:Lysates:Overexpression Lysates D
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Introduction The amounts below are given for a 12-well plate format. Use Table 1 to adjust the reagent volumes for other plate sizes
DOK1 overexpression lysate, 0.1 mg. Transient overexpression lysate of docking protein 1, 62kDa (downstream of tyrosine kise 1) (DOK1)
Addgene NGS Result GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTT AAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACA ACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCG ATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTC ATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCC AAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTA TGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGC AGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACG CAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCA CTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTGGTACCGAGCTCGGAT ...
0361] Cell Line Specific Transfection Reagents/Kits include, e.g., GenJet® (Ver. II) DNA In Vitro Transfection Reagent for 3LL Cell, TransIT®-3T3 Transfection Kit, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for NIH3T3 Cell, Human B Cell Nucleofector® Kit, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for B16-F10 Cells, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for BHK-21 Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for C6 Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for C6 Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for Ca Ski Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for Caco-2 Cell, DG44 Transfection Kit, TransIT®-CHO Transfection Kit, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for CHO Cell, TransIT®-COS Transfection Kit, TransPass® COS Transfection Reagent, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for COS Cell, Targefect-COS, GenJet® (Ver. II) DNA In Vitro Transfection Reagent ...
Carbon dots (CDs) have attracted great attention in a broad range of applications due to its unique optical properties, high biocompatibility and property adjustability. However, developed CDs can be rarely used as effective gene vectors up to now. In this work, a fluorine-doping strategy was provided to prepare a novel fluorine-doped CDs (F-CDs) using fluorine-containing aromatic compound as fluorine source and using branched polyethyleneimine (PEI) to furnish positive charge sites. The as-synthesized F-CDs achieves dramatic gene transfection efficiency as well as low cytotoxicity in commonly used cell lines at low weight ratio. It is worthy to point out that this F-CDs is superior efficiency and better biocompatibility compared to some widely used commercial reagents such as branched 25k Da PEI and Lipofectamine 2000. At weight ratio (transfection reagent/EGFP plasmid) of 2, the transfection efficiency of F-CDs achieves about 92% in HEK 293T cells, 85% in COS-7 cells, 81% in A549 cells, 73% in ...
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... : Product & Service Citations - > In Vitro DNA Transfection Reagents > In Vivo Transfection Reagents > Pre-optimized Transfection Reagents > In Vitro siRNA Transfection Reagents > Transfection Accessories > Pre-made Adenovirus > shRNA/miRNA AAV Service > Adenovirus Production Service > shRNA/miRNA Adenovirus Svc > AAV Production Service > Ready-to-package Adenovirus > Pre-made AAVs > Pre-made Lentivirus > Lentivirus Production Service SignaGen Laboratories, DNA/siRNA Transfection Reagents, Custom Adenovirus Production Service, Custom AAV Production Service, Custom Lentivirus Production Service
SAN FRANCISCO, Aug. 13, 2019 /PRNewswire/ -- Global Transfection Technologies Market is expected to grow at a significant CAGR in the upcoming period as the scope and its applications are rising enormously across the globe. Transfection technology is used to introduce nucleic acids like RNA or DNA into cells. It helps to regulate gene therapy, protein metabolism, and mutation of cancer cells by affecting the nuclear genes.. The factors that are playing a major role in the growth of transfection technologies market are the rise in research and development sector, the rising investment by private manufacturers and by the government, and the growing occurrence of cancer. However, the high cost of instruments may restrain the overall market growth in the years to come. Market of Transfection technologies is segmented based on type, application, and region.. The siRNA delivery, gene delivery, protein delivery, and DNA delivery are the types that could be explored in transfection technologies market ...