The first three zinc fingers (ZF1-3) of transcription factor IIIA (TFIIIA) from Xenopus have been shown to contribute the majority of the binding energy to the intact TFIIIA-DNA interaction [Liao et al. (1992) J. Mel. Biol, 223, 857-871]. We have expressed a 92-amino acid polypeptide containing the three N-terminal zinc fingers of TFIIIA. This three-fingered polypeptide has
And Ish-Horowicz, D. Individual stripe regulatory elements in the Drosophila hairy promoter respond to maternal, gap, and pair-rule genes. Genes Dev. 5,840-854. , and Jiickle, H. Structural homology of the product of the Drosophila Kruppel gene with Xenopus transcription factor IIIA. Nature (London) 319,336-339. Rushlow, C. , Pinchin, S. , Howe, K. , and Ish-Horowicz, D. The Drosophila hairy protein acts both in segmentation and bristle p a t terning and shows homology to N-myc. 8,3095-3103. Salz, H. VI. Response of the Neural Plate to Induction . . . . . . . . A . Transcription Factors . . . . . . . . . . . . B. wntGenes . . . . . . . . . . . . . . . C. Structural and Physiological Proteins of the Nervous System . . . D. Perspective . . . . . . . . . . . . . . . VII . Conclusions . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . 29 30 30 33 43 44 44 45 45 47 48 48 48 51 53 53 53 54 55 56 57 58 59 65 66 68 68 69 1. This may not seem too unusual given the bias of the screens. ...
This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.[7] ...
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [(PUBMED:10529348), (PUBMED:15963892), (PUBMED:15718139), (PUBMED:17210253), (PUBMED:12665246)]. Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domains are often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both ...
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [(PUBMED:10529348), (PUBMED:15963892), (PUBMED:15718139), (PUBMED:17210253), (PUBMED:12665246)]. Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domains are often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both ...
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [(PUBMED:10529348), (PUBMED:15963892), (PUBMED:15718139), (PUBMED:17210253), (PUBMED:12665246)]. Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domains are often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both ...
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Shop Optineurin ELISA Kit, Recombinant Protein and Optineurin Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Mutations in this gene have been associated with adult-onset primary open-angle glaucoma (POAG). [provided by RefSeq, Jul 2008 ...
Discussion. The autosomal dominant mode of inheritance was apparent in the eight pedigrees. In this study, 28% of the first degree relatives to the glaucoma patients were classified as glaucoma suspects. Their age at examination was lower than the age at diagnosis of the glaucoma patients. Their possible risk to develop glaucoma will be doubled every decade [19] in addition to their genetic risk [3]. For this group of patients the identification of the glaucoma gene/genes would be especially beneficial providing a probability with a DNA test to identify a possible glaucoma patient earlier and thus to lower a risk for a possible visual handicap in the future.. Two genes, TIGR/MYOC and OPTN, are known to be associated with POAG. In TIGR, more than 50 mutations have been identified, the majority of them being associated with juvenile onset glaucoma (JOAG). However, mutations in TIGR have also been found in elderly population with POAG [5]. Recently, optineurin gene (OPTN) was identified at ...
1. Summarize the primary purpose of an internal control system? 2. What are the three internal control objectives for financial reporting? 3. What are the accounting regulatory bodies, and discuss how the organization complies.
A zinc finger motif is an element of proteins that can specifically recognize and bind to DNA. Because they contain multiple cysteine residues, zinc finger motifs possess redox properties. Ionizing radiation generates a variety of free radicals in organisms. Zinc finger motifs, therefore, may be a target of ionizing radiation. The effect of gamma radiation on the zinc finger motifs in transcription factor IIIA (TFIIIA), a zinc finger protein, was investigated. TFIIIA was exposed to different gamma doses from 60Co sources. The dose rates were 0.20 Gy/min and 800 Gy/h, respectively. The binding capacity of zinc finger motifs in TFIIIA was determined using an electrophoretic mobility shift assay. We found that 1000 Gy of gamma radiation impaired the function of the zinc finger... motifs in TFIIIA. The sites of radiation-induced damage in the zinc finger were the thiol groups of cysteine residues and zinc (II) ions. The thiol groups were oxidized to form disulfide bonds and the zinc (II) ions were ...
Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008 ...
Our data indicate that M98K is not a risk factor for POAG by itself. However, at least among French POAG patients, M98K was associated positively with a low initial IOP and negatively with a high initial IOP. Therefore, it can be considered as a modifier of the glaucoma phenotype. This influence of the OPTN gene on IOP is consistent with its expression in the trabecular meshwork.6 Alternatively, as OPTN seems to interfere with induction of apoptosis,6,11 the M98K variant could increase the sensitivity of ganglial neural cells to risk factors of glaucoma. Consequently, in the presence of M98K, a lesser elevation of IOP would be required to damage the optic nerve.. Interestingly, other clinical features were not altered by the presence of M98K. Thus, patients positive or negative for M98K did not differ at diagnosis in age, magnitude of cupping of the optic disc, severity of visual field loss or response to IOP lowering treatments (data not shown).. Despite its low frequency, the M98K variant ...
Molecular Genetics Studies of GlaucomaGlaucoma is a group of blinding inherited ocular disorders that affects over 70 million people worldwide. The clinical manifestation of this condition extends from birth to 80 years of age. Over the last 2 decades, our laboratory has identified and published 8 genetic loci for this condition {i.e., GLC3A, GLC3B, GLC3C for Primary Congenital Glaucoma (PCG); GLC1B, GLC1D, GLC1E, GLC1G and GLC1H for Primary Open Angle Glaucoma (POAG)} and further cloned 4 Glaucoma-causing genes {i.e., CYP1B1 (Cytochrome P450 1B1) as the first "PCG" gene; Optineurin (For Optic Neuropathy Inducing protein; OPTN) as the first "Normal Tension" Adult-Onset POAG gene; also WDR36 (WD40 Repeat Protein) and GLC1D for "High Tension" Adult-Onset POAG}. Over the years, we utilized a series of in vivo and in vitro experiments to determine the functional significance of these genes in the etiology of glaucoma. We cloned the orthologue of OPTN gene in both mouse and Rhesus monkey and further ...
Transcription factor and ribosomal RNA (rRNA). Molecular model showing the 6 zinc fingers of transcription factor IIIA (purple) bound to RNA (ribonucleic acid, pink-beige and green) from a 5s ribosome sub-unit. Transcription factors are proteins that bind to specific DNA sequences, and control the movement (transcription) of genetic information from DNA to mRNA (messenger RNA) during gene expression. Ribosomes are responsible reading the RNA strand and assembling amino acids to form the protein encoded by the gene being read. - Stock Image C008/8514
Transcription factor and ribosomal RNA (rRNA). Molecular model showing the 6 zinc fingers of transcription factor IIIA (yellow) bound to RNA (ribonucleic acid, red and blue) from a 5s ribosome sub-unit. Transcription factors are proteins that bind to specific DNA sequences, and control the movement (transcription) of genetic information from DNA to mRNA (messenger RNA) during gene expression. Ribosomes are responsible for reading the RNA strand and assembling amino acids to form the protein encoded by the gene being transcribed. - Stock Image F006/9530
The Company has implemented and is continuously improving a centralized risk management and internal control model.. The effective functioning of the risk management and internal control system allows ensuring the proper control of the Companys financial and economic activities and providing the Companys management with a set of appropriate tools to identify, assess, manage and monitor risks, as well as to exchange the necessary information between stakeholders in order to make risk-oriented management decisions.. The Companys risk management and internal control system complies with the requirements of the Russian legislation, recommendations of the Corporate Governance Code of the Bank of Russia and best practices and represents a set of risk management and internal control processes implemented on the basis of the existing organizational structure, internal policies and regulations, procedures and methods of risk management and internal control applied in the Company at all levels of ...
Proper risk management and internal control help organizations understand the risks they are exposed to, put controls in place to counter threats, and effectively pursue their objectives. They are therefore an important aspect of an organizations governance, management, and operations. Professional accountants can and should play a leading role in helping their organizations achieve an integrated, organization-wide approach to risk management and internal control-which ultimately helps create, enhance, and protect stakeholder value. See the full overview.. ...
Proper risk management and internal control help organizations understand the risks they are exposed to, put controls in place to counter threats, and effectively pursue their objectives. They are therefore an important aspect of an organizations governance, management, and operations. Professional accountants can and should play a leading role in helping their organizations achieve an integrated, organization-wide approach to risk management and internal control-which ultimately helps create, enhance, and protect stakeholder value. See the full overview.. ...
Internal controls and auditing case study from CPA and consulting firm Accounting and Business Consultants, LLC. Serving public companies in the Northeast.
FINMA operates an effective internal control system in order to minimise organisational weaknesses, optimise its own processes and identify risks.
Aggregation of misfolded proteins and the associated loss of neurons are considered as a hallmark of numerous neurodegenerative diseases. Optineurin is present in protein inclusions observed in various neurodegenerative diseases including amyotrophic lateral sclerosis (ALS), Huntingtons disease, Alzheimers disease, Parkinsons disease, Creutzfeld-Jacob disease and Picks disease. Optineurin deletion mutations have also been described in ALS patients. However, the role of optineurin in mechanisms of protein aggregation remains unclear. In this report, we demonstrate that optineurin recognized various protein aggregates via its C-terminal coiled-coil domain in a ubiquitin-independent manner. We also show that optineurin depletion significantly increase protein aggregation in HeLa cells and morpholino-silencing of the optineurin ortholog in zebrafish causes the motor axonopathy phenotype similar to a zebrafish model of ALS. A more severe phenotype is observed when optineurin is depleted in ...
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Internal controls case study for an oil company from CPA and consulting firm Accounting and Business Consultants, LLC. Serving clients in PA, NJ, DE and throughout the Northeast.
INTERNAL CONTROL SYSTEM IN A COMPUTERIZED ACCOUNTING ENVIRONMENT (A Case Study Of Quiness Nigeria Plc), Largest Undergraduate Projects Repository, Research Works and Materials. Download Undergraduate Projects Topics and Materials Accounting, Economics, Education
The OPTN is operated under contract with the U.S. Dept. of Health and Human Services by the United Network for Organ Sharing (UNOS). This Web site provides data and educational information about organ donation, transplantation and the matching process.
The OPTN is operated under contract with the U.S. Dept. of Health and Human Services by the United Network for Organ Sharing (UNOS). This Web site provides data and educational information about organ donation, transplantation and the matching process.
Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms. Alongside CRISPR/Cas9 and TALEN, ZFN is a prominent tool in the field of genome editing. The DNA-binding domains of individual ZFNs typically contain between three and six individual zinc finger repeats and can each recognize between 9 and 18 basepairs. If the zinc finger domains perfectly recognize a 3 basepair DNA sequence to generate a 3-finger array that can recognize a 9 basepair target site. Other procedures can utilize either 1-finger or 2-finger modules to generate zinc-finger arrays with six or more individual zinc fingers. The main drawback with ...
Glaucoma is a group of disorders with a broad range of clinical and histopathological manifestations. This condition presents itself in many different ways and with distinct shared characteristics that include optic neuropathy due to optic nerve head damage (cupping) and visual field dysfunction with or without presence of increased intraocular pressure (lOP). Glaucoma is the leading cause of irreversible blindness, which afflicts nearly 67 million people worldwide. It is considered the second most frequent cause of bilateral blindness, affecting 6.7 million people worldwide. By 2020, glaucoma is anticipated to affect 79.6 million people, increasing the number of bilaterally blind individuals due to glaucoma to 11.1 million. This thesis aimed to provide new insights into the molecular genetics of adult-onset Primary Open Angle Glaucoma (POAG) and a paediatric form of Primary Congenital Glaucoma (PCG). In the first portion of this work, the GLC1B locus on chromosome 2pl1.2-qI2.2 was investigated ...
The Internal Controls and Risk Management Specialist plays a key role in ensuring that the USGs humanitarian assistance and disaster risk reduction programs are effective and efficient. The Internal Controls and Risk Management Specialist supports OFDAs efforts to strengthen internal controls throughout OFDAs global operations, implement and fully utilize the Agencys Enterprise Risk Management (ERM) framework and tools, and coordinate and track OFDAs ongoing audits and open recommendations. The Internal Controls and Risk Management Specialist assists in analyzing the effectiveness of existing and newly implemented internal controls, creating and documenting new internal controls, responding to Federal Managers Financial Integrity Act (FMFIA) requirements, tracking corrective action plans, and monitoring significant internal control and risk weaknesses.. ...
In this study, we designed and introduced Top1 siRNA constructs into human cell lines to establish siTop1 cell lines from two different tissues of origin (colon carcinoma HCT116-siTop1 and breast carcinomas MCF-7-siTop1). Both siTop1 cell lines show a stable ∼5-fold reduction of Top1 expression. However, neither of the siTop1 clones lacked Top1, consistent with the idea that, in contrast to yeast ( 2), human cells require Top1 [as do mouse and fly embryos ( 6, 7)]. The studies reported here provide the first direct lines of evidence for nonclassic roles of human Top1 in maintaining genomic stability, regulating gene-specific transcription and determining response to anticancer drugs.. Human Top1 and genomic integrity. Previous studies have shown that Top1 inhibitors can induce deletion mutations ( 30), chromosomal aberrations ( 31), and replication double-strand breaks ( 10). However, using Top1 inhibitors (e.g., CPT) cannot appropriately define the role of Top1 in genomic stability because ...
The International Federation of Accountants has released a document highlighting the best practices for business accountants to follow in terms of effective governance, risk management and internal control.
Internal control has emerged from isolation. In recent years, according to an International Federation of Accountants (IFAC) report, internal control has come to be viewed as an integral part of risk management and governance rather than a separate concept unto itself. This integration demands that individual risks be assessed holistically
This course provides the details of a good fraud deterrence initiative and looks at the three main types of cash fraud schemes and examines how to prevent them.
1) What do you do if you are unable to determine internal controls? 2) Which do you think is most important? The two types of audit tests are tests of controls and the substantive tests. The tests of controls are tests that.
Need to strip the membran before probe the internal control protein - posted in Protein and Proteomics: Hello! I am new in the proteomic area. I need to do a quantitative western blot and use Actin as internal control. I am not sure how to blot probe two proteins in one membrane (my target protein and Actin). Can I cut out the area for Actin (~50kDa) and probe anti-Actin antibody and probe the other area with my target antibody? Or do I need to first probe my target protein and strip...
The Companys Board of Directors and management are aware and give precedence to internal control system as a crucial factor to create confidence and minimize business risks which enhances efficiency to serve the company objectives by allocating companys resources appropriately as planned.. The Board of Directors has assigned the Audit Committee to effectively and appropriately regulate the companys internal control and risk management. In other words, the Audit Committee has to supervise the company to follow the related laws and regulations in order to prevent conflicts of interests, or connected transactions. Apart from that, the Audit Committee also needs to monitor and control the companys operation, and to prevent illegitimate or unauthorized uses of companys properties. In addition to that, the Audit Committee has to prevent the companys assets from missing, loss, misconduct or corruption. The company has established audit mechanisms by having internal audit independently preformed ...
Webhelp group has adopted the definition set forth by the Committee of Sponsoring Organizations of the Treadway Commission (COSO) in Internal Control...
Purpose: Optineurin (OPTN) is a multifunctional protein and its E50K mutation responsible for primary open angle glaucoma (POAG) and amyotrophic lateral sclerosis (ALS). We have previously reported our E50K transgenic mouse (E50K-tg) exhibits thinner retina and retinal ganglion cell loss, while the underlying molecular mechanisms have been unclear. Together with additional phenotypes of E50K-tg retina and over expression studies, the endogenous OPTN and E50K mutant protein dynamics was investigated by iPS Cell (iPSC) from E50K-POAG patients to elucidate the fundamental pathoetiology.. Methods: E50K-tg retina were examined by using anti-GFAP, anti-OPTN, and anti-HA antibodies. The intracellular localization and protein property of endogenous OPTN in wild type control and in E50K carrier were also examined by iPSCs and iPSC-derived neural cells. FLAG-tagged OPTN and E50K were expressed in HEK293T cells and protein complex formation/oligimerization was examined by Native-PAGE and LC-MS/MS ...
This Heads Up discusses considerations related to a companys internal control over financial reporting in connection with its adoption of the FASBs new standard on revenue recognition.
As you work on a more global scale and expand your operations you will outsource, relocate and work with subsidiaries. This means you need to adapt the way that you evaluate performance, shareholder profitability and improve transparency.
Pza. San Diego, s/n. 28801 - Alcalá de Henares (Madrid). Spain. Información:+34 91 885 50 00 [email protected] Centralita: +34 91 885 40 00. ...
Differential expression of the oocyte and somatic 5 S RNA genes during Xenopus development can be explained by changes in transcription factor and histone interactions with the two types of gene. Both factors and histones bind 5 S RNA genes with specificity. Protein-protein interactions determine the stability of potentially transcriptionally active or repressed nucleoprotein complexes. A decline in transcription factor abundance, differential binding of transcription factors to oocyte and somatic 5 S genes, and increased competition with the histones for association with DNA during early embryogenesis, can account for the developmental decision to selectively repress the oocyte genes, while retaining the somatic genes in the transcriptionally active state. The 5 S ribosomal genes of Xenopus are perhaps the simplest eukaryotic genes to show regulated expression during development. A large multigene family (oocyte 5 S DNA) is transcriptionally active in oocytes but is repressed in somatic cells, ...
A cartoon that discusses some internal controls over bank reconciliations in a local church: The cartoon is based on the book Once Upon Internal Control - A tale of good and bad ways to implement internal controls in a local church, written by James Ulvog, CPA. If you want more detail, the video above is…
Background: In the public sector scandals involving bribes, corruption and other irregularities are occasionally observed. Many organizations are therefore currently focusing on internal control. For internal control to achieve full power, good policy documents are required. We therefore find it interesting to study the policies and guidelines on Internal Controls in the public sector.. Purpose: The purpose of this report is to study the design of policies for internal control in Uddevalla municipality. We also intend to study the organizations work with the guidelines within different levels of the municipality.. Method and implementation: Semi-structured interviews are the basis for our empirical work, where we chose to interview people who are involved in internal control or otherwise have leading positions and hence are important in the process of internal control. We have also examined the governing documents on the municipal website and intranet to gain as much insight as possible ...
The protein encoded by this gene plays a role in ribosomal protein transport and in the assembly of the 5S ribonucleoprotein particle (5S RNP). The encoded protein also may be involved in NOD2-mediated NF-kappaB signaling. [provided by RefSeq, Jul 2016 ...
The Department of Labor administers the unemployment insurance program in New York State. Financial information about this program is reported to the Office of the State Comptroller for inclusion in New York State s comprehensive annual financial report. We audited the written support for the Department s internal controls over this information and found that this written support was adequate. Consequently, if the controls are followed, there is a reduced risk of significant misstatements in the financial information included in the State s comprehensive annual financial report ...
Rabbit polyclonal antibody raised against a full-length human OPTN protein. OPTN (NP_001008212.1, 1 a.a. ~ 577 a.a) full-length human protein. (H00010133-D01P) - Products - Abnova