TY - JOUR. T1 - Neutrophil recruitment by intradermally injected neutrophil attractant/activation protein-1. AU - Leonard, Edward J.. AU - Yoshimura, Teizo. AU - Tanaka, Shuji. AU - Raffeld, Mark. PY - 1991/5. Y1 - 1991/5. N2 - Neutrophil attractant/activation protein-1 (NAP-1) is a recently described cytokine that attracts neutrophils, but not monocytes or eosinophils. This leukocyte specificity is not absolute, in that NAP-1 attracts basophils and small numbers of lymphocytes. Our purpose was to determine in vivo effects of NAP-1, and to compare them to the reported action of the complement attractant, C5a. Intradermal injection into normal human subjects of 40 μl of NAP-1, over a concentration range of 4 × 10-8 M to 10-6 M, caused no symptoms or signs such as wheal-and-flare, itching, induration, or tenderness. However, biopsies of injection sites showed perivascular neutrophil infiltration as early as 30 min, which increased at 1 and 3 h. The mean number of neutrophils per mm2 of dermis ...
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Homo sapiens transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) (TFAP2A), transcript variant 2, mRNA. (H00007020-R01V) - Products - Abnova
J:138599 Hong SJ, Lardaro T, Oh MS, Huh Y, Ding Y, Kang UJ, Kirfel J, Buettner R, Kim KS, Regulation of the noradrenaline neurotransmitter phenotype by the transcription factor AP-2beta. J Biol Chem. 2008 Jun 13;283(24):16860-7 ...
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Gene duplication has been proposed to drive the evolution of novel morphologies. After gene duplication, it is unclear whether changes in the resulting paralogs coding-regions, or in their cis-regulatory elements, contribute most significantly to the assembly of novel gene regulatory networks. The Transcription Factor Activator Protein 2 (Tfap2) was duplicated in the chordate lineage and is essential for development of the neural crest, a tissue that emerged with vertebrates. Using a tfap2-depleted zebrafish background, we test the ability of available gnathostome, agnathan, cephalochordate and insect tfap2 paralogs to drive neural crest development. With the exception of tfap2d (lamprey and zebrafish), all are able to do so. Together with expression analyses, these results indicate that sub-functionalization has occurred among Tfap2 paralogs, but that neo-functionalization of the Tfap2 protein did not drive the emergence of the neural crest. We investigate whether acquisition of novel target ...
In this study, we also analyzed binding of lung nuclear proteins from sham-operated control and trauma rats with AP-1, Egr-1, and NF-κB probes specific for the regulatory region of the rat TF gene using electrophoretic mobility shift assays. Sequence-specific complex binding to AP-1 and NF-κB domains was shown to be significantly enhanced in the lung 2 h after trauma. Because the 5′-region of the rat TF gene contains two, closely spaced AP-1 elements, we incubated the nuclear proteins with different AP-1 probes. Both AP-1 sites include core sequence elements, TGAATCA (distal) and TGAGTCA (proximal), that resemble the consensus binding site of the AP-1 family of transcription factors. Oeth et al. 10 demonstrated that the two AP-1 sites bind c-Fos/c-Jun heterodimers in unstimulated and lipopolysaccharide-stimulated human monocytic cells. However, Groupp and Donovan-Peluso 40 demonstrated that lipopolysaccharide induces a change in the AP-1 binding from JunD/Fos in control THP-1 monocytes to ...
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear ...
The regulation of macrophage activator protein-1 (AP-1) gene expression by LPS and cytokines is of potentially crucial importance in the pathogenesis of several diseases. The action of LPS and four cytokines on AP-1 gene expression in the murine macrophage J774.2 cell line was, therefore, studied. Exposure of the cells to IL-6 produced no changes in the mRNA levels of all AP-1 members studied. In contrast, the expression of JunB, c-jun and c-fos, but not JunD, was increased by LPS, TNF-α, IFN-γ and IL-1, albeit with different kinetics and magnitude of induction. Electrophoretic mobility shift assays showed a close correlation between the expression of the AP-1 genes and the functional AP-1 DNA binding activity and, additionally, demonstrated the participation of heterodimeric interactions between the different members. These studies provide insights into the potential mechanisms that may be involved in the mediator-specific modulation of AP-1 regulated macrophage gene expression.. ...
Carcinogenesis is caused by a cumulative, multistage process that mainly consists of initiation, promotion, and progression. ROS, which are produced as a result of the metabolism of molecular oxygen via biochemical reactions in cells, play a key role in tumor promotion. Some tumor promoters accelerate/induce the conversion of initiated cells (carcinogen-mediated mutation or potential "stem cells") into tumorigenic cells possibly via the production of oxidative/inflammatory responses (30, 31). TPA (12-O-tetradecanoylphorbol-13-acetate), a phorbol ester, is a tumor promoter that induces the neoplastic/tumorigenic transformation of preneoplastic JB6 cells through the overproduction of ROS (32). In this study, we investigated the inhibitory effect of SFN on TPA-stimulated neoplastic transformation in the mouse epidermal JB6 P+ cell line to assess whether SFN is able to block tumor promoter-induced tumorigenesis in skin cells. Our results show that SFN was effective when it was given together with ...
TransAM AP-1 Kits are DNA-binding ELISAs that quantify activated c-Fos, FosB, c-Jun, JunB, JunD & Fra-1 transcription factors using a method that is faster and more sensitive than gelshift, without radioactivity and gels.
Plasmid NFAT/AP-1 3x luciferase from Dr. Anjana Raos lab contains the insert NFAT/AP-1 3x and is published in EMBO J. 2000 Sep 1. 19(17):4783-95. This plasmid is available through Addgene.
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TY - JOUR. T1 - Hydrogen peroxide-induced liver cell necrosis is dependent on AP-1 activation. AU - Yang, X. U.. AU - Bradham, Cynthia. AU - Brenner, David A.. AU - Czaja, Mark J.. PY - 1997. Y1 - 1997. N2 - To deter-mine whether intracellular signaling events involved in apoptosis may also mediate necrosis, the role of the transcription factor AP-1 was investigated in a hepatoma cell model of cellular necrosis induced by oxidant stress. Treatment of the human hepatoma cell line HuH-7 with H2O2 caused dose-dependent necrosis as determined by light microscopy, fluorescent staining, and an absence of DNA fragmentation. H2O2 treatment led to increases in c-fos and c-jun mRNA levels, Jun nuclear kinase activity, and AP-1 DNA binding. AP-1 transcriptional activity measured with an AP-1-driven luciferase reporter gene was also increased. To determine whether this AP-1 activation contributed to H2O2-induced cell necrosis, HuH-7 cells were stably transfected with an antisense c-jun expression vector. ...
CORVELLO, C M; METZ, R; BRAVO, R; ARMELIN, M C S. Expression and characterization of mouse cfos protein using the baculovirus expression system: ability to form functional ap-1 complex with co-expressed c jun protein. Cellular and Molecular Biology Research, New York, v. 41, p. 527-35, 1996 ...
AP-12 Analog Phaser by Synapse Audio (@KVRAudio Product Listing): The Synapse AP-12 rack extension emulates vintage analog phasers, which are composed of a series of phase shifting stages with a feedback path wrapped around them. The phase shifting stages (switchable between 6 and 12 stages) are modulated by a LFO, which can be synchronized to the current song tempo. In contrast to most hardware phasers, the AP-12 employs a stereo signal path, allowing the LFO t...
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References for Abcams Recombinant Human c-Jun protein (ab54319). Please let us know if you have used this product in your publication
AP-1 Is a Component of the Transcriptional Network Regulated by GSK-3 in Quiescent Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
binding to the DR-1/AP-1 site in suppressing MMP-1 and MMP-13 production and addressed possible mechanisms of inhibition, including competitive binding between RXR:PPAR ...
Among environmental pollutants, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; dioxin) is one of the most potent tumor promoters and teratogens known. The molecular mechanisms responsible for the biological activity of TCDD, however, remain largely unknown. In this report, we show that the first observable effects of TCDD in cultured murine hepatoma cells are a rapid, transient increase in Ca2+ influx and a minor but significant elevation of activated, membrane-bound protein kinase C. These changes are then followed by induction of the immediate early proto-oncogenes c-fos, jun-B, c-jun, and jun-D, and by large increases in AP-1 transcription factor activity. Induction of these changes by TCDD is delayed compared with that by phorbol esters, although the magnitude of the effects caused by both treatments is similar, and both induction processes can be blocked by staurosporine, a protein kinase C inhibitor. In cultured cells, proto-oncogene induction by TCDD appears to be independent of the presence of a
Heparin is a potent inhibitor of the proliferation and migration of vascular smooth muscle cells. This agent selectively inhibits the transcription of tissue-type plasminogen activator and interstitial collagenase, probably by decreasing the binding of activator protein-1 (AP-1) to phorbol ester-responsive elements in the promoters of these genes. Decreased AP-1 binding is not due to a direct inhibition by heparin, since heparinase digestion of nuclear extracts prepared from heparin-treated smooth muscle cells does not restore AP-1 binding activity. Treatment of cells with heparin suppresses the expression of Jun B, one of the components of AP-1. The major effect of heparin is at the level of posttranslational modification of Jun B. Results from pulse-chase labeling experiments show that the newly synthesized Jun B is rapidly converted to a higher-molecular-weight form and that conversion is suppressed by heparin. Evidence is presented suggesting that the heparin-inhibited event is ...
Chameau HAT and DRpd3 HDAC function as antagonistic cofactors of JNK/AP-1-dependent transcription during Drosophila metamorphosis
Constitutive expression of c-Fos, FosB, Fra-1, or c-Jun in rat fibroblasts leads to up-regulation of the immediate-early gene fra-1. Using the posttranslational FosER induction system, we demonstrate that this AP-1-dependent stimulation of fra-1 expression is rapid, depends on a functional DNA-binding domain of FosER, and is a general phenomenon observed in different cell types. In vitro mutagenesis and functional analysis of the rat fra-1 gene in stably transfected Rat-1A-FosER fibroblasts indicated that basal and AP-1-regulated expression of the fra-1 gene depends on regulatory sequences in the first intron which comprise a consensus AP-1 site and two AP-1-like elements. We have also investigated the transactivating and transforming properties of the Fra-1 protein to address the significance of fra-1 up-regulation. The entire Fra-1 protein fused to the DNA-binding domain of Ga14 is shown to lack any transactivation function, and yet it possesses oncogenic potential, as overexpression of Fra-1 ...
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c-Fos兔多克隆抗体(ab429)可与鸡样本反应并经WB, EMSA实验严格验证,被6篇文献引用并得到1个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
Kharat SS, Tripathi V, Damodaran AP, Priyadarshini R, Chandra S, Tikoo S, Nandhakumar R, Srivastava V, Priya S, Hussain M, Kaur S, Fishman JB, Sengupta S ...
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5-GCCNNNGGC-3 and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region ...
Quercetin (QUE; 3,5,7,3′,4′-tetrahydroxyflavone) has been shown to possess several beneficial biological activities including antitumor, anti-inflammation and antioxidant properties; however, the effects of QUE in preventing invasion by breast carcinoma cells are still undefined. Increases in the protein, messenger RNA and enzyme activity levels of matrix metalloproteinase (MMP)-9 were observed in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 cells, and these were blocked by QUE, but not by quercitrin or rutin. A translocation of protein kinase C (PKC)δ from the cytosol to the membrane followed by activation of extracellular signal-regulated kinase (ERK) and c-Jun/activator protein-1 (AP-1) by TPA was demonstrated, and TPA-induced MMP-9 activation and migration were inhibited by the pan PKC inhibitor, GF109203X, the specific PKCδ inhibitor, rottlerin, an ERK inhibitor (PD98059) and an AP-1 inhibitor (curcumin). Application of QUE significantly suppressed TPA-induced activation ...
This chapter focuses on these modifications, which take place in the absence of protein synthesis, and on auxiliary factors involved in modulating activator
The targetPROFILER allows to monitor activities of G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs) under diverse stimuli conditions. Individual signaling events, such as dimerisations and adaptor recruitment, can be simultaneously analysed using the EXTassay technology.. Additional signaling events, like transcription factor activities, can also be included and addressed in one experiment using the deepPROFILER.. ...
The AP-1 (activator protein-1) complex, which consists of proteins of the Fos and Jun families, is thought to play an important role in the balance between cell proliferation and apoptosis, the response to genotoxic stress ...
Complete information for JUND gene (Protein Coding), JunD Proto-Oncogene, AP-1 Transcription Factor Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Buy our Recombinant Human c-Jun protein. Ab54318 is a full length protein produced in Escherichia coli and has been validated in WB, SDS-PAGE. Abcam provides…
AP-1 transcriptional activity is stimulated by the transformation promoters phorbol 12-myristate 13-acetate ("12-O-tetradecanoylphorbol 13-acetate," TPA) and epidermal growth factor (EGF) in promotion-sensitive (P+) but not in promotion-resistant (P-) JB6 mouse epidermal cell lines. Although TPA stimulates expression of the jun and fos family genes, only c-jun expression shows higher elevation in P+ cells than in P- cells. The present study tests the hypothesis that induced AP-1 activity is required for tumor promoter-induced transformation in JB6 P+ cells. Both retinoic acid and the glucocorticoid fluocinolone acetonide inhibited basal and TPA-induced AP-1 activities that were tested with a stromelysin promoter-chloramphenicol acetyltransferase reporter gene in P+ cells. Since both retinoic acid and fluocinolone acetonide are active in inhibiting TPA-induced anchorage-independent transformation of P+ cells in the dose range that blocks TPA-induced AP-1 activity, their antipromoting effects may ...
Transcription factor AP-2 alpha (AP-2α or TFAP2A) is a newly identified prognostic marker of chemotherapy; its expression is positively correlated with chemosensitivity and survival of cancer patients. Using computational programs, we predicted that the coding region of AP-2α gene contains a potential miRNA response element (MRE) of miR-193a-5p, and the single nucleotide polymorphism (SNP) site (c.497A,G, rs111681798) resides within the predicted MRE. The results of luciferase assays and Western blot analysis demonstrated that miR-193a-5p negatively regulated the expression of AP-2α proteins, but have no influence on the mutant AP-2α (c.497A,G). Infection with lentiviral AP-2α gene or miR-193a-5p inhibitor in the bladder cancer cells decreased migration and cisplatin resistance, while knockdown of AP-2α gene or overexpression of miR-193a-5p in the urothelial cell line SV-HUC-1 increased migration and cisplatin resistances. We concluded that miR-193a-5p induced cisplatin resistance by ...
View Notes - Lec20 from BCH 110 at UC Riverside. Lecture 20 Eukaryotic Gene Regulation 2 REGULATORY TRANSCRIPTION FACTORS & ACTIVATION MECHANISMS Lodish 6th edition Chapter 7 Lodish 5th edition
We demonstrate that JunD, a component of the AP-1 transcription factor complex, activates transcription of the human proenkephalin gene in a fashion that is completely dependent upon the cAMP-dependent protein kinase, protein kinase A. Activation of proenkephalin transcription by JunD is dependent upon a previously characterized cAMP-, phorbol ester-, and Ca(2+)-inducible enhancer, and JunD is shown to bind the enhancer as a homodimer. Another component of the AP-1 transcription complex, JunB, is shown to inhibit activation mediated by JunD. As a homodimer JunB is unable to bind the enhancer; however in the presence of c-Fos, high-affinity binding is observed. Furthermore, JunD is shown to activate transcription of genes linked to both cAMP and phorbol ester response elements in a protein kinase A-dependent fashion, further blurring the distinction between these response elements. These results demonstrate that the transcriptional activity of an AP-1-related protein is regulated by the ...
immune Activator Protein-1 152044-53-6 supplier, Mouse monoclonal to CD152 Many research showed a potential anti-tumor role for cannabinoids, by modulating cell signaling pathways included in cancer cell proliferation, migration and chemo-resistance. mixture, had been capable to decrease cell viability by causing autophagic-dependent necrosis. Furthermore, we demonstrated that the CBD-THC mixture was capable to decrease Millimeter cells migration by down-regulating appearance of 152044-53-6 supplier the chemokine receptor CXCR4 and of the Compact disc147 plasma membrane layer glycoprotein. Furthermore, since the immuno-proteasome is definitely regarded as a fresh focus on in Millimeter and also since carfilzomib (CFZ) is definitely a fresh encouraging immuno-proteasome inhibitor that creates permanent adducts with the 5i subunit of immuno-proteasome, we examined the impact of CBD and THC in controlling the appearance of the 5i subunit and their impact in mixture with CFZ. Herein, we also ...
Complete information for SALL2 gene (Protein Coding), Spalt Like Transcription Factor 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Vitamin C as a potent antioxidant. Environmental factors, such as solar radiation, pollution, and smoking can accelerate damage to the skin through the generation of so-called "oxidative stress". Vitamin C is one of the most potent antioxidants in the skin. It neutralizes the oxidative stress by a process of electron transfer and/or donation.[3]. Protection against photoaging. Transurocanic acid is a by-product of fillagrin present in the skin, which acts as a chromophore for photons of solar radiation (mainly ultraviolet [UV] and, to a degree, infrared) leading to the formation of singlet oxygen. This triggers a cascade of events that lead to the formation of so-called "reactive oxygen species" or "free radicals."[7-9] Free radicals are highly toxic, unstable molecules that can cause damage to nucleic acids, proteins, and cell membranes. UV-induced reactive oxygen species also trigger the signal transduction cascade, which leads to upregulation of factors, such as activation protein-1 (AP-1) ...
The epidermal growth factor receptor (EGFR) family regulates essential natural processes. and function of Elk-1 recruiting it towards the TBP promoter. On the other hand EGFRvIII robustly induces c-jun appearance rousing recruitment of c-fos/c-jun for an overlapping AP-1 site. Improving c-jun appearance by itself induces TBP promoter activity through the AP-1 site. To look for […]. ...
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Stress-responsive activator protein. Molecular model of the stress-responsive activator of p300 (strap) protein. This protein is activated when certain types of DNA (deoxyribonucleic acid) damage occur. It activates a number of regulatory proteins. - Stock Image F006/9740
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The AP-1 transcription factor, composed of Jun and Fos proteins, plays a crucial role in the fine tuning of cell proliferation. We showed previously that AP-1 complexes are activated during the proliferative response that parallels the development of renal lesions after nephron reduction, but little is known about the specific role of individual Jun/Fos components in the deterioration process. Here we used JunD knockout (JunD-/-) mice and an experimental model of chronic renal injury (75% nephron reduction) to explore the role of JunD. Nephron reduction resulted in an initial compensatory growth phase that did not require JunD. JunD, however, was essential to inhibit a second wave of cell proliferation and to halt the development of severe glomerular sclerosis, tubular dilation, and interstitial fibrosis. We show that the effects of junD inactivation are not cell autonomous and involve upregulation of the paracrine mitogen, TGF-α. Expression of a transgene (REM) encoding a dominant negative ...
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