BACKGROUND: Multiple sclerosis (MS) is a complex trait in which alleles at or near the class II loci HLA-DRB1 and HLA-DQB1 contribute significantly to genetic risk. The MHC class II transactivator (MHC2TA) is the master controller of expression of class II genes, and methylation of the promoter of this gene has been previously been shown to alter its function. In this study we sought to assess whether or not methylation of the MHC2TA promoter pIV could contribute to MS disease aetiology. METHODS: In DNA from peripheral blood mononuclear cells from a sample of 50 monozygotic disease discordant MS twins the MHC2TA promoter IV was sequenced and analysed by methylation specific PCR. RESULTS: No methylation or sequence variation of the MHC2TA promoter pIV was found. CONCLUSION: The results of this study cannot support the notion that methylation of the pIV promoter of MHC2TA contributes to MS disease risk, although tissue and timing specific epigenetic modifications cannot be ruled out.
STAT3 antibody (signal transducer and activator of transcription 3 (acute-phase response factor)) for ICC/IF, IHC-P, WB. Anti-STAT3 pAb (GTX50709) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
STAT5A (phospho Tyr694) antibody [5F6.F1] (signal transducer and activator of transcription 5A) for ELISA, ICC/IF, IHC-P, WB. Anti-STAT5A (phospho Tyr694) mAb (GTX48647) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Lapin STAT1 Polyclonal anticorps pTyr701 pour ELISA, WB. Publier en 3 références Pubmed. Order anti-STAT1 anticorps ABIN539531.
Abcam provides specific protocols for Anti-STAT6 antibody (ab88540) : Western blot protocols, Immunocytochemistry & immunofluorescence protocols
anti-STAT2, pAb is a polyclonal antibody that crossreacts with human protein. Works in WB. Important for Inflammation, Oxidative Stress, ROS, Immunology research.
Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315) has been cited in 56 publications. References for Human, Mouse, Rat in IHC, IHC-Fr, IHC-P, WB
引用Abcams Anti-STAT1 (phospho S727)抗体(ab4742)的参考文献列表。为您列举引用本产品的发表文章,并提供信息包括论文文献数据库中的检索编号以便您搜寻文章
优异的STAT3兔单抗(ab76315)经WB, IP, IHC-P, ICC实验严格验证,可用于小鼠, 大鼠和人。被多篇文献引用并有多个独立的用户反馈。中国75%以上现货。
Polyclonal antibody for STAT1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. STAT1 information: Molecular Weight: 87335 MW; Subcellular Localization: Cytoplasm . Nucleus . Translocated into the nucleus upon tyr
Polyclonal antibody for STAT2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. STAT2 information: Molecular Weight: 97916 MW; Subcellular Localization: Cytoplasm. Nucleus. Translocated into the nucleus upon activ
Monoklonale und polyklonale STAT5B Antikörper für viele Methoden. Ausgesuchte Qualitäts-Hersteller für STAT5B Antikörper. Hier bestellen.
Mouse monoclonal STAT1 antibody [SM1] validated for WB, IP, IHC, Flow Cyt and tested in Human and Mouse. Referenced in 5 publications and 3 independent…
高い抗原親和性、特異性と安定した品質を兼ね備えたアブカムのウサギ・モノクローナル抗体 RabMAb® ab109320 交差種: Hu 適用: WB,IP,IHC-P,ICC,Flow Cyt
Looking for online definition of haematopoietic transcription factor PU.1 in the Medical Dictionary? haematopoietic transcription factor PU.1 explanation free. What is haematopoietic transcription factor PU.1? Meaning of haematopoietic transcription factor PU.1 medical term. What does haematopoietic transcription factor PU.1 mean?
Hepatitis B virus X protein targets the Bcl-2 protein CED-9 to induce intracellular Ca2+ increase and cell death in Caenorhabditis elegans Journal Article ...
The mammalian hepadnaviruses encode an HBx protein that may function as a weak transactivator of a variety of viral and cellular promoter and enhancer elements. In addition, the ability of HBx to alter signaling pathways involved in cellular growth and differentiation as well as apoptosis has led to the hypothesis that continuous low-level HBx expression due to persistent viral infection of the liver may contribute to the development of HCC. However, the biologic function(s) of HBx during natural HBV infection is not known. The HBx protein structure does not contain a defined DNA binding motif, nuclear localization signal, or any other protein domains that yield clues to its cellular localization or function in virus-infected hepatocytes. Studies of the molecular mechanism(s) by which HBx functions with respect to modulation of viral replication have been hindered by the fact that HBx expression occurs at very low levels in naturally HBV infected hepatocytes as well as in HCC cells transfected ...
Class II transactivator (CIITA) is a global transcriptional coactivator of human leukocyte antigen-D (HLA-D) genes. CIITA contains motifs similar to guanosine triphosphate (GTP)-binding proteins. This report shows that CIITA binds GTP, and mutations in these motifs decrease its GTP-binding and transactivation activity. Substitution of these motifs with analogous sequences from Ras restores CIITA function. CIITA exhibits little GTPase activity, yet mutations in CIITA that confer GTPase activity reduce transcriptional activity. GTP binding by CIITA correlates with nuclear import. Thus, unlike other GTP-binding proteins, CIITA is involved in transcriptional activation that uses GTP binding to facilitate its own nuclear import. ...
insulin promoter factor 1: PDX-1/IPF1/STF-1/IDX-1/IUF-1 is a homeodomain containing transcription factor; found in pancreatic beta-cells; RIPE3b1 was cloned and identified as the mammalian homologue of an avian regulator of cellular differentiation MafA/L-Maf; amino acid sequence in first source; RerSeq NM_008814 (mouse), NM_013311 (human), NM_022852
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Rabbit Polyclonal Anti-Stat4 (phospho Tyr693) antibody (STJ90753). From Abcams OEM supplier St Johns Laboratory, validated in WB, IHC, ELISA. AntibodyPlus provides trial size antibody samples for antibody validation. Replacement to Abcam, Santa Cruz, Sigma and CST antibody.
The transcription factor PU.1 is often impaired in patients with acute myeloid leukemia (AML). Here, we used AML cells that already had low PU.1 levels and further inhibited PU.1 using either RNA interference or, to our knowledge, first-in-class small-molecule inhibitors of PU.1 that we developed specifically to allosterically interfere with PU.1-chromatin binding through interaction with the DNA minor groove that flanks PU.1-binding motifs. These small molecules of the heterocyclic diamidine family disrupted the interaction of PU.1 with target gene promoters and led to downregulation of canonical PU.1 transcriptional targets. shRNA or small-molecule inhibition of PU.1 in AML cells from either PU.1lo mutant mice or human patients with AML-inhibited cell growth and clonogenicity and induced apoptosis. In murine and human AML (xeno)transplantation models, treatment with our PU.1 inhibitors decreased tumor burden and resulted in increased survival. Thus, our study provides proof of concept that ...
Abstract. Abstract 2333The gene locus BX648577 (FLJ27352/hypothetical LOC145788) was recently identified as part of a gene fusion with Class II Transactivator (
RBMS1 overexpression lysate, 0.1 mg. Transient overexpression lysate of R binding motif, single stranded interacting protein 1 (RBMS1), transcript variant 2
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TY - JOUR. T1 - MHC class II transactivator represses human IL-4 gene transcription by interruption of promoter binding with CBP/p300, STAT6 and NFAT1 via histone hypoacetylation. AU - Zhou, Xiaorong. AU - Jiang, Yang. AU - Lu, Liming. AU - Ding, Qing. AU - Jiao, Zhijun. AU - Zhou, Yun. AU - Xin, Lijun. AU - Chou, Kuang Yen. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2007/12. Y1 - 2007/12. N2 - In addition to its property of enhancing major histocompatibility complex (MHC) class II expression, the class II transactivator (CIITA) was recently demonstrated to be involved in T helper type 1/type 2 (Th1/Th2) differentiation by regulating interleukin-4 (IL-4) gene transcription. There was however, controversy regarding whether CIITA promotes or suppresses IL-4 expression in the experiments with transgenic mice. To clarify the discrepancy by using simpler experimental systems, human Jurkat T cells that express IL-4 but not interferon-γ, even if stimulated with phorbol ...
TY - JOUR. T1 - The pancreatic duodenal homeobox-1 protein (Pdx-1) interacts with histone deacetylases Hdac-1 and Hdac-2 on low levels of glucose. AU - Mosley, Amber L.. AU - Özcan, Sabire. PY - 2004/12/24. Y1 - 2004/12/24. N2 - We have previously demonstrated that high concentrations of glucose stimulate insulin gene expression by causing hyperacetylation of histone H4 at the insulin gene promoter. Furthermore, we have shown that the glucose-mediated hyperacetylation of histone H4 depends on the recruitment of the histone acetyltransferase p300 by the beta cell-specific transcription factor Pdx-1. In this study, we demonstrate that the histone deacetylases Hdac-1 and Hdac-2 are rapidly recruited to the insulin promoter in the mouse insulinoma cell line MIN6 when cells are switched from high to low glucose media. Moreover, we demonstrate that the beta cell-specific homeodomain protein Pdx-1 interacts with histone deacetylases Hdac-1 and Hdac-2 at low levels of glucose. In vitro studies indicate ...
The role of hepatitis B virus (HBV) X protein (HBx) in the regulation of HBV replication remains controversial. In the present study, the role of HBx in regulating HBV replication was initially investigated in both HepG2 and Huh7 in vitro cell lines with a transient transfection system. Next, the regions of HBx responsible for transcriptional transactivation and promotion of HBV replication were mapped in an HBV replication mouse model by in vivo transfection of a series of HBx expression plasmids. In an in vitro setting, HBx deficiency had little effect on HBV replication in Huh7 cells, but impaired HBV replication in HepG2 cells. In an in vivo setting, HBx had a strong enhancing effect on HBV transcription and replication. For the C-terminal two-thirds of the protein (amino acids [aa] 51 to 154) was required for this function of HBx, and the regions spanning aa 52 to 72 and 88 to 154 were found to be important for the stimulatory function of HBx on HBV replication. In conclusion, the role of HBx in
The role of hepatitis B virus (HBV) X protein (HBx) in the regulation of HBV replication remains controversial. In the present study, the role of HBx in regulating HBV replication was initially investigated in both HepG2 and Huh7 in vitro cell lines with a transient transfection system. Next, the regions of HBx responsible for transcriptional transactivation and promotion of HBV replication were mapped in an HBV replication mouse model by in vivo transfection of a series of HBx expression plasmids. In an in vitro setting, HBx deficiency had little effect on HBV replication in Huh7 cells, but impaired HBV replication in HepG2 cells. In an in vivo setting, HBx had a strong enhancing effect on HBV transcription and replication. For the C-terminal two-thirds of the protein (amino acids [aa] 51 to 154) was required for this function of HBx, and the regions spanning aa 52 to 72 and 88 to 154 were found to be important for the stimulatory function of HBx on HBV replication. In conclusion, the role of HBx in
TY - JOUR. T1 - Characterization of distinct Stat5b binding sites that mediate growth hormone-stimulated IGF-I gene transcription. AU - Chia, Dennis J.. AU - Ono, Mitsuru. AU - Woelfle, Joachim. AU - Schlesinger-Massart, Mylynda. AU - Jiang, Honglin. AU - Rotwein, Peter. PY - 2006/2/10. Y1 - 2006/2/10. N2 - A key agent in the anabolic actions of growth hormone (GH) is insulin-like growth factor-I (IGF-I), a 70-amino acid secreted protein with direct effects on somatic growth and tissue maintenance and repair. GH rapidly and potently stimulates IGF-I gene transcription by mechanisms independent of new protein synthesis, and recent studies have linked the transcription factor Stat5b to a regulatory network connecting the activated GH receptor on the cell membrane to the IGF-I gene in the nucleus. Here we analyze two distinct conserved GH response elements in the rat IGF-I locus that contain paired Stat5b sites. Each response element binds Stat5b in vivo in a GH-dependent way, as assessed by ...
The JAK (Janus kinase)/STAT (signal transducer and activator of transcription) pathway, a recently discovered signaling pathway utilized by many cytokines and growth factors, was first elucidated in the context of interferon (IFN) signaling (11). It was later discovered that a large number of cytokines and growth factors, including most if not all of those that act through the cytokine receptor superfamily, activate overlapping sets of STAT family members, often in addition to activating other signaling pathways (11). IFN-γ signaling remains, however, a canonical example (2, 56). IFN-γ binding mediates IFN-γ receptor chain aggregation, which activates two cytoplasmic tyrosine kinases belonging to the JAK family, Jak1 and Jak2, that associate with the cytoplasmic face of the IFN-γ receptor chains. Upon receptor oligomerization, the JAKs phosphorylate each other and Tyr440 of the IFN-γ receptor α chain. Then Stat1, a latent cytoplasmic transcription factor that is a member of the STAT gene ...
Signal transducers and activators of transcription (STATs) are latent, cytoplasmic transcription factors. Janus kinases (JAKs) and activated CDC42-associated kinase-1 (ACK1/TNK2) catalyse the phosphorylation of STAT1 and the expression of its target genes. Here we demonstrate that catalytically active ACK1 promotes the phosphorylation and nuclear accumulation of STAT1 in transformed kidney cells. These processes are associated with STAT1-dependent gene expression and an interaction between endogenous STAT? and ACK1. Moreover, the E3 ubiquitin ligase seven-in-absentia homolog-2 (SIAH2), which targets ACK1 through valine-909 for proteasomal degradation, attenuates the ACK1-STAT1 signalling node. We further show that ACK1 promotes the phosphorylation and nuclear accumulation of STAT3 in cultured cells and that the levels of ACK1 correlate positively with the levels of tyrosine phosphorylated STAT3 in primary lung adenocarcinoma (ADC) cells. Global analysis of ACK1 interaction partners validated the ...
Background: A number of clinical trials using stem/progenitor cell transplantation in ischemic heart diseases are now on-going, however, aging-induced cell dysfunction may impair therapeutic efficacy. We tested the hypothesis that genetic modification of EPCs by sonic hedgehog (Shh), one of the embryonic morphogens, may ameliorate the loss of function in aged EPCs.. Methods and Results: Cultured EPCs were isolated from 3m.o. and 24m.o. mice and cell functions, including cytokine expression, were evaluated by real-time PCR. Proliferation, migration and adhesion activity were significantly decreased and apoptosis was increased in the 24m.o.-EPCs vs. 3m.o.-EPCs. The reduced expression of VEGF, eNOS, and IGF-1 were also observed in the 24m.o.-EPCs. We then evaluated the effect of Shh gene transfer to the 24m.o.-EPCs. Shh gene transfer significantly improved proliferation and anti-apoptosis activities in 24m.o.-EPCs vs. empty vector transfected (EV) 24m.o.-EPCs, up to a similar level of EV ...
Mouse Anti-STAT5B Monoclonal Antibody (1H5) - This product is mouse monoclonal antibody recognizes STAT5B of human. The antibody 1H5 immunoassay techniques such as: IP, MA, WB.
Complete information for CIITA gene (Protein Coding), Class II Major Histocompatibility Complex Transactivator, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
J:117226 Wei K, Che N, Chen F, Myocardin-related transcription factor B is required for normal mouse vascular development and smooth muscle gene expression. Dev Dyn. 2007 Feb;236(2):416-25 ...
[ Hcc Chemistry Lab Manual ] - Boiling And Melting Points Study Questions Lab 3 Bioling And,Full Text Mir 663 Overexpression Induced By Endoplasmic Reticulum,Nuclear Expression Of Hepatitis B Virus X Protein Is Associated
Clone REA272 recognizes the N-terminus of the signal transducer and activator of transcription 1-α/β (STAT1) antigen, regardless of phosphorylation status. STAT1 is expressed in two alternatively spliced isoforms (91 kDa STAT1α and 84 kDa STAT1β) and clone REA272 recognizes both isoforms. STAT1 expression is found ubiquitously. It is involved in upregulating genes due to a signal by either type I, type II, or type III interferons. In response to interferon γ (IFN-γ) stimulation, the STAT1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. STAT1 forms homodimers or heterodimers with STAT3 that bind to the IFN-γ activated sequence promoter element. In response to either IFN-α or IFN-β stimulation, STAT1 forms a heterodimer with STAT2 that can bind the interferon stimulated response promoter element. In either case, binding of the promoter element leads to an increased expression of interferon stimulated genes. Additional information: Clone REA272
The Ets-Related Gene (ERG) belongs to the Ets family of transcription factors and is critically important for maintenance of the hematopoietic stem cell population. A chromosomal translocation observed in the majority of human prostate cancers leads to the aberrant overexpression of ERG. We have identified regions flanking the ERG Ets domain responsible for autoinhibition of DNA binding and solved crystal structures of uninhibited, autoinhibited, and DNA-bound ERG. NMR-based measurements of backbone dynamics show that uninhibited ERG undergoes substantial dynamics on the millisecond-to-microsecond timescale but autoinhibited and DNA-bound ERG do not. We propose a mechanism whereby the allosteric basis of ERG autoinhibition is mediated predominantly by the regulation of Ets-domain dynamics with only modest structural changes. Structural and dynamic studies of the transcription factor ERG reveal DNA binding is allosterically autoinhibited.,Regan MC, Horanyi PS, Pryor EE Jr, Sarver JL, Cafiso DS, ...
Targeting of the tetracycline transactivator gene to the BF1 locus. The targeting construct was made by replacing thelacZ sequence in the BF1 targeting vector previously described (Xuan et al., 1995) with the sequence encoding the tetracycline transactivator (tTA) from pUHD 15-1 (Gossen and Bujard, 1992). The SalI site in the tTA sequence was eliminated by mutagenesis. The tTA sequence was inserted between aSalI-ApaI fragment containing the BF1promoter and an EcoRI-KpnI fragment containing an SV40 intron and poly(A) sequence (Hebert and McConnell, 2000). TheSalI-BamHI fragment was then inserted into theSalI-BamHI sites in the pHBL3 plasmid. Linearized targeting vector was electroporated into W9.5 embryonic stem (ES) cells. Targeted ES clones were identified by PCR and Southern blot as previously described. Three correctly targeted clones were injected into blastocysts to generate chimeric mice that were bred with C57/BL6 mice.. Generation and screening of tetO transgenic lines.IRES3lacZ was ...
PTCH1 [ENSP00000332353]. Protein patched homolog 1; Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehogs proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis; Belongs to the patched family.. Synonyms: PTCH1, PTCH1p, hPTCH1, A3KBF6, F8VPA3 .... Linkouts: STRING Pharos UniProt OMIM ...
Following the publication of this article [1], the authors found that the primers listed for CREB-binding protein were not correct. This mistake occurred during assembly of the primer table and the authors apologize for this error. This correction does not change the data included in the paper, their interpretation nor the conclusions drawn.
Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs…
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Components of the signaling pathways that lie downstream of Ser/Thr kinase receptors and are required for signaling by the TGF beta superfamily have been poorly defined. The Drosophila gene Mothers against dpp (MAD) and the C. elegans sma genes are implicated in these signaling pathways. We show tha …
Regulation of gene transcription Ub conjugation affects gene transcription because many transcription factors become conjugated to Ub, and transcription activators are degraded by the proteasome [16 ...
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cdna chromosome:GRCm38:10:23104763:23349887:-1 gene:ENSMUSG00000010461 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Eya4 description:EYA transcriptional coactivator and phosphatase 4 [Source:MGI Symbol;Acc:MGI:1337104 ...
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