Molar Incisor Hypomineralization or MIH is a developmental condition seen in children presenting as enamel defects in the permanent incisors and the first
Modified glass ionomer and orthodontic band: An interim alternative for the treatment of molar incisor hypomineralization. A case report.
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Osteomalacia is the softening of the bones caused by impaired bone metabolism primarily due to inadequate levels of available phosphate, calcium, and vitamin D, or because of resorption of calcium. The impairment of bone metabolism causes inadequate bone mineralization. Osteomalacia in children is known as rickets, and because of this, use of the term "osteomalacia" is often restricted to the milder, adult form of the disease. Signs and symptoms can include diffuse body pains, muscle weakness, and fragility of the bones. In addition to low systemic levels of circulating mineral ions necessary for bone and tooth mineralization, accumulation of mineralization-inhibiting proteins and peptides (such as osteopontin and ASARM peptides) occurs in the extracellular matrix of bones and teeth, likely contributing locally to cause matrix hypomineralization (osteomalacia).[1][2][3][4][5] The most common cause of osteomalacia is a deficiency of vitamin D, which is normally derived from sunlight exposure and, ...
Figure 3: Lingual shelf (MW) and dentin (DA2/DA1) layers in place. The patient had two types of subtle white calcifications occurring on their natural enamel, which needed to be replicated in composite for ultimate esthetic blending of the final restoration. One type is a very soft and diffuse white splotchiness along the line angles and is best created using a bleach-white composite, which can be more easily feathered for the diffuse appearance. In this case, the BL1 composite works well, as it is a highly opaque white but can be feathered nicely. The BL1 composite was initially placed with a composite instrument, and excess composite was removed until a reasonable amount remained (Fig 4). Feathering and diffusing of the BL1 composite were then achieved using the OptraSculpt instrument (Ivoclar Vivadent), which has a small foam pad that makes it easy to wipe away the BL1 composite until the desired effect is achieved. ...
SLC26A Gene Family Participate in pH Regulation during Enamel Maturation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Polyclonal antibody for Alkaline Phosphatase/ALPL detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Alkaline Phosphatase/ALPL information: Molecular Weight: 57305 MW; Subcellular Localization: Cell membrane ; L
Rabbit polyclonal antibody raised against partial recombinant human ALPL. Recombinant protein corresponding to amino acids 198-316 of human ALPL. (PAB30563) - Products - Abnova
Published in: Premières Journées JCSEE1 - Chimie, soleil, énergie et environnement, Saint-Avold, France, 3-4 févr. 2000, p. 10 ...
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Hypophosphatasia (also called deficiency of alkaline phosphatase or phosphoethanolaminuria) is a rare, and sometimes fatal, metabolic bone disease. Clinical symptoms are heterogeneous, ranging from the rapidly fatal, perinatal variant, with profound skeletal hypomineralization and respiratory compromise, to a milder, progressive osteomalacia later in life. Tissue non-specific alkaline phosphatase (TNSALP) deficiency in osteoblasts and chondrocytes impairs bone mineralization, leading to rickets or osteomalacia. The pathognomonic finding is subnormal serum activity of the TNSALP enzyme, which is caused by one of 200 genetic mutations identified to date, in the gene encoding TNSALP. Genetic inheritance is autosomal recessive for the perinatal and infantile forms but either autosomal recessive or autosomal dominant in the milder forms. The prevalence of hypophosphatasia is not known; one study estimated the live birth incidence of severe forms to be 1:100,000. There is a remarkable variety of ...
Insulin-dependent type-1 diabetes mellitus (DM) and oral diseases are closely interrelated. Poor metabolic control in diabetics is associated with a high risk of gingivitis, periodontitis and tooth loss. Salivary flow declines in diabetics and patients suffer from xerostomia. Reduced saliva predisposes to enamel hypomineralization and caries formation; however, the mechanisms that initiate and lead to progression of tooth decay and periodontitis in type-1 DM have not been explored. To address this issue, we analyzed tooth morphology in Akita-/- mice that harbor a point mutation in the Ins2 insulin gene which leads to insulin deficiency and hyperglycemia. Objective: To characterize the tooth phenotype in Akita-/- mice and determine whether hyperglycemia and hyposalivation contribute to dental defects. Methods: Mandibles from Akita-/- and wild type littermates were analyzed by microCT, scanning EM and histology; teeth were examined for amelogenin and ameloblastin expression. Mice were injected ...
The pulp periphery: At the outer border of the pulp, odontoblasts and the so-called Hoehls cells form continuous layers. These postmitotic cells have the capacity to undergo terminal differentiation. Odontoblasts are implicated in the synthesis of collagen and noncollagenous extracellular matrix components. Some ECM proteins are phosphorylated (SIBLINGs), whereas others are non-phosphorylated. ECM components are implicated in predentin and dentin formation, followed by dentin mineralization. Due to a fixation artifact, the formation of a cell-free layer results in from fixation and dehydration. A cell-free area underlines odontoblasts and Hoehls cells, which do not appear on sections after adequate fixative perfusion. Fenestrated capillary loops infiltrate the layer formed by odontoblasts and Hoehls cells but do not cross the terminal junctions located between the distal odontoblast cell bodies nor penetrate within the predentin. In contrast, axons infiltrate the odontoblastic layer and ...
... is a genetic condition in which the activity of an enzyme called alkaline phosphatase is deficient. This enzyme plays an essential role in mineralization - when minerals such as calcium and phosphorus are deposited in developing bones and teeth. Low activity of this enzyme which characterizes hypophosphatasia also leads to rickets, osteomalacia, or both. The severity of the symptoms of hypophosphatasia varies widely, from fetal loss during pregnancy to cases in which pathologic fractures first present only in adulthood. Hypophosphatasia is caused by a mutation in a gene - called the ALPL gene - that provides instructions for making the alkaline phosphatase enzyme. A cure or proven medical therapy for hypophosphatasia has not yet been found. Treatment is generally directed towards preventing or correcting the symptoms or complications ...
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Hypophosphatasia is a heterogeneous disease and the various forms of the disease can vary a great deal in severity. Mortality is high for the most severe forms in children with symptoms already at the foetal stage and before the age of 6 months. Individuals with the least severe form of hypophosphatasia may not be diagnosed until they reach adulthood ...
It is the consequence of interspecies competition and is occasionally thought of as serial succession. There are three major types of interdependence. It appears to be another illustration of asymmetry.. Not getting sufficient sleep could possibly be linked to stress and guilt. Many packaged foods are irradiated so as to denature bacterial proteins and guarantee that the food can be kept for lengthy amounts of time. There are many types of barometers.. Secondly, dehydration reactions can be categorized on the essence of the catalyst. This kind of mimicry is extremely rare and harder to understand than the previous two different types. This kind of denaturation http://www.medschool.umaryland.edu/CVD/ is very important to transcription and DNA replication.. Polarity in the creation of the embryo could be illustrated by the early maturation of the nematode embryo. Principal succession is a type of ecological succession that refers to succession where there isnt any substrate available. Psoriatic ...
It is the consequence of interspecies competition and is occasionally thought of as serial succession. There are three major types of interdependence. It appears to be another illustration of asymmetry.. Not getting sufficient sleep could possibly be linked to stress and guilt. Many packaged foods are irradiated so as to denature bacterial proteins and guarantee that the food can be kept for lengthy amounts of time. There are many types of barometers.. Secondly, dehydration reactions can be categorized on the essence of the catalyst. This kind of mimicry is extremely rare and harder to understand than the previous two different types. This kind of denaturation http://www.medschool.umaryland.edu/CVD/ is very important to transcription and DNA replication.. Polarity in the creation of the embryo could be illustrated by the early maturation of the nematode embryo. Principal succession is a type of ecological succession that refers to succession where there isnt any substrate available. Psoriatic ...
Baby marmosets that were introduced to solid food at a young age are more likely to become obese, and to eat larger quantities of food if given the chance, according to a new study. This early-life obesity resulted in a host of metabolic changes, including insulin resistance, a hallmark of type 2 diabetes. "With its small size and early maturation, we think the marmoset is going to be an exceptionally good model of early life obesity and offers many opportunities to further explore why youngsters become obese," said Suzette D. Tardif, associate professor of cellular and structural biology at the University of Texas-San Antonio School of Medicine. Tardifs team had noticed obesity patterns began at a young age in these small monkeys, and wanted to test whether marmosets could become overweight by simply eating more. Tardif found that patterns leading to obesity begin very early in young marmosets, around 30 days of age. A month-old marmoset is equivalent to a 5- to 8-month-old human. The ...
... _mineral formation and identifi ion process - Grinding chinagrindingmill mineral formation and identifi ion processMineral Identifiion Flow Chart The Science Queen Mineral Identifiion Flow C
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Background. The goal of treating exposed pulp with an appropriate pulp capping material is to promote the dentinogenic potential of the pulpal cells. There have been recent attempts to develop more effective pulp-capping materials. Objectives. The aim of this study was to evaluate the effect of newly developed calcium silicate-based material on odontogenic differentiation of primary human dental pulp cells (HDPCs), in comparison with a contemporary calcium silicate-based material. Material and methods. Human dental pulp cells isolated from dental pulps were cultured in standard culture conditions in Dulbeccos Modified Eagles Medium (DMEM) and then the effects of Micro-Mega mineral trioxide aggregate (MM-MTA) (Micro-Mega, Besancon, France) and ProRoot MTA (MTA) (Dentsply Sirona, Tulsa, USA) (positive control) were evaluated on HDPCs at 1, 7 and 14 days. Untreated cells were used as a negative control. Odontoblastic differentiation was assessed by alkaline phosphatase (ALP) activity. Runtrelated ...
This study aimed to characterize fluoride-induced alterations in dentin mineralization within a dentin-pulp organ culture system. Tooth sections derived from male Wistar rat incisors were cultured in Trowel-type culture for 14 days, in the presence of 0 mM, 1 mM, 3 mM and 6 mM sodium fluoride. Tooth sections were processed and analyzed for uptake of fluoride, its subsequent effect on dentin mineralization by tetracycline hydrochloride incorporation and mineral composition, expressed as calcium/phosphorous (Ca/P) ratios. Tetracycline hydrochloride incorporation was demonstrated to decrease with increased fluoride exposure, accompanied by significant increases in both Ca/P ratios and fluoride incorporation. These findings provide further evidence that the established alterations in dentin formation during fluorosis are a consequence of disruption to the mineralization process, and provide a model system with which to investigate further the potential role the extracellular matrix plays in inducing ...
Damage or exposure of the dental pulp requires immediate therapeutic intervention. This study assessed the biocompatibility of a silver-containing PLGA/TCP-nanofabric scaffold (PLGA/Ag-TCP) in two in vitro models, i.e. the material adapted on pre-cultured cells and cells directly cultured on the material, respectively. Collagen saffolds with and without hyaluronan acid (Coll-HA; Coll) using both cell culturing methods and cells growing on culture plates served as reference. Cell viability and proliferation were assessed after 24, 48, and 72 h based on formazan formation and BrdU incorporation. Scaffolds were harvested. Gene expression of interleukin(IL)-6, tumor necrosis factor (TNF)-alpha, and alkaline phosphatase (AP) was assessed 24 h after stimulation. In both models formazan formation and BrdU incorporation was reduced by PLGA/Ag-TCP on dental pulp cells, while no significant reduction was found in cells with Coll and Coll-HA. Cells with PLGA/Ag-TCP for 72 h showed similar relative BrdU
The hereditary dentine disorders, dentinogenesis imperfecta (DGI) and dentine dysplasia (DD), comprise a group of autosomal dominant genetic conditions characterised by abnormal dentine structure affecting either the primary or both the primary and secondary dentitions. DGI is reported to have an incidence of 1 in 6,000 to 1 in 8,000, whereas that of DD type 1 is 1 in 100,000. Clinically, the teeth are discoloured and show structural defects such as bulbous crowns and small pulp chambers radiographically. The underlying defect of mineralisation often results in shearing of the overlying enamel leaving exposed weakened dentine which is prone to wear. Currently, three sub-types of DGI and two sub-types of DD are recognised but this categorisation may change when other causative mutations are found. DGI type I is inherited with osteogenesis imperfecta and recent genetic studies have shown that mutations in the genes encoding collagen type 1, COL1A1 and COL1A2, underlie this condition. All other forms of
Enamel demineralization is frequently encountered in dental practice. Histologically, hypomineralization is the common feature and several etiologies stand behind their clinical appearance. Recently, resin infiltration technique was introduced to mask these enamel lesions.
Research and study of fluoride ingestion by children relating to the causes and risks of enamel fluorosis. Read our research and article summaries.
Cell viability rate (%) according to NR assay in human dental pulp cells (hDPCs) exposed to MTA Plus (MTAP), MTA Fillapex (MTAF), FillCanal (FC) and culture med
Introduction: Mutations in the gene ALPL in hypophosphatasia (HPP) reduce the function of tissue nonspecific alkaline phosphatase, and the resulting increase in pyrophosphate (PPi) contributes to bone and tooth mineralization defects by inhibiting physiologic calcium-phosphate (P-i) precipitation. Although periodontal phenotypes are well documented, pulp/dentin abnormalities have been suggested in the clinical literature although reports are variable and underlying mechanisms remains unclear. in vitro analyses were used to identify mechanisms involved in HPP-associated pulp/dentin phenotypes. Methods: Primary pulp cells cultured from HPP subjects were established to assay alkaline phosphatase (ALP) activity, mineralization, and gene expression compared with cells from healthy controls. Exogenous P-i was provided to the correct P-i/PPi ratio in cell culture. Results: HPP cells exhibited significantly reduced ALP activity (by 50%) and mineral nodule formation (by 60%) compared with the controls. ...
Principal Investigator:NAGASAKA Nobuo, Project Period (FY):1996 - 1997, Research Category:Grant-in-Aid for Scientific Research (B), Section:一般, Research Field:矯正・小児・社会系歯学
Expression of ALPL (HOPS, TNSALP) in pancreas tissue. Antibody staining with HPA007105, HPA008765 and CAB020829 in immunohistochemistry.
Embedding nano size CoFe2O4 ferrite particles in a hydroxyapatite (HAP) host would make them to better candidates for hyperthermia treatment of cancer. The formation of nano crystallites of CoFe2O4 ferrites in the hydroxyapatite Ca10-3xFe2xCox(PO4)6(OH)2 (where x= 0, 0.1, 0.2, 0.3, 0.4 and 0.5 %mole) mixtures heated to temperatures between 500 and 1250°C for 2 hrs.
i6006886, also known as c.299C,T or p.T100M, is a SNP in the ALPL gene on chromosome 1. Based on the ALPL gene mutations database, the rare/minor allele is considered pathogenic for the infantile form of hypophosphatasia. ...
Theres chronological age and performance age and both may not be the same. So what is age? Some maintain age is a physical state, others believe it has
Maintaining or regenerating a vital pulp is a preferable goal in current endodontic research. In this study, human dental pulp cell aggregates (spheres) were applied onto bovine and human root canal models to evaluate their potential use as pre-differentiated tissue units for dental pulp tissue regeneration. Human dental pulp cells (DPC) were derived from wisdom teeth, cultivated into three-dimensional cell spheres and seeded onto bovine and into human root canals. Sphere formation, tissue-like and mineralization properties as well as growth behavior of cells on dentin structure were evaluated by light microscopy (LM), confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). Spheres and outgrown cells showed tissue-like properties, the ability to merge with other cell spheres and extra cellular matrix formation; CLSM investigation revealed a dense network of actin and focal adhesion contacts (FAC) inside the spheres and a pronounced
Serum calcium levels are tightly controlled by an integrated hormone-controlled system that involves active vitamin D [1,25(OH)2D], which can elicit calcium mobilization from bone when intestinal calcium absorption is decreased. The skeletal adaptations, however, are still poorly characterized. To gain insight into these issues, we analyzed the consequences of specific vitamin D receptor (Vdr) inactivation in the intestine and in mature osteoblasts on calcium and bone homeostasis. We report here that decreased intestinal calcium absorption in intestine-specific Vdr knockout mice resulted in severely reduced skeletal calcium levels so as to ensure normal levels of calcium in the serum. Furthermore, increased 1,25(OH)2D levels not only stimulated bone turnover, leading to osteopenia, but also suppressed bone matrix mineralization. This resulted in extensive hyperosteoidosis, also surrounding the osteocytes, and hypomineralization of the entire bone cortex, which may have contributed to the ...
Enamel fluorosis is a defect in enamel development that occurs after exposure to excess fluoride. Fluorotic enamel is more porous, and contains more proteins th...
The aim of this study was to examine whether brownish crown and root discoloration of wisdom teeth was related to treatment of acne with tetracyclines. For this purpose, 17 discolored third molars from nine patients were embedded without being decalcified, ground along the tooth axis, and examined using fluorescence microscopy. A thorough medical history served to determine the start and duration of any administration of tetracyclines. This confirmed the use of drugs against acne containing minocycline in all cases except one. The microscopic analyses of all teeth revealed intensely fluorescent bands in the dentin, which corresponded to the mineralization front at the time of tetracycline intake. More or less uniform discoloration of the entire crown was seen in association with treatment against acne prior to the completion of crown formation at the age of about 15 years. This uniform staining can be attributed to incorporation of minerals during ongoing maturation of the occlusal enamel, which ...
Hypophosphatasia is a rare inherited metabolic disease of decreased tissue nonspecific alkaline phosphatase (TNSALP) and defective bone mineralization. Both autosomal recessive and autosomal dominant variants of the disease exist. The disease comes in one of five forms, perinatal, infantile, childhood, adult, and odontohypophosphatasia. Perinatal hypophosphatasia is invariably lethal while infantile hypophosphatasia has a roughly 50% mortality rate with symptoms appearing within the first 6th months after birth. The other forms are generally non-lethal. Common symptoms include bone malformations and higher chance of bone fracture. Both the adult form and odontohypophosphatasial form are marked by premature teeth loss. There is no known cure for hypophosphatasia. However, there have been some claims that choline may have positive health benefits for those with the disease that take it as a dietary supplement. ...
The dentin-enamel junction (DEJ), which is the interface between the dentin and outer enamel coating in teeth, is known for its unique biomechanical properties that provide a crack-arrest barrier for flaws formed in the brittle enamel. In this work, we re-examine how cracks propagate in the proximity of the DEJ, and specifically quantify, using interfacial fracture mechanics, the fracture toughness of the DEJ region. Additionally, we show that the vital function of the DEJ, in preventing cracks formed in enamel from traversing the interface and causing catastrophic tooth fractures, is not necessarily associated with the crack-arrest capabilities of the DEJ itself, but rather with the development of crack-tip shielding, primarily from uncracked-ligament bridging, in the mantle dentin adjacent to the DEJ. Measurements of the toughness of the DEJ region give estimates of Gc ~; 115 J/m2, i.e., ~;5 to 10 times higher than enamel and ~;75 percent of that of dentin. ...
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A research group led by Professor Hannes Lohi at the University of Helsinki and Folkhälsan Research Center has uncovered a new skeletal disease in dogs. The disease was recognized in the Karelian Bear Dog breed and associated with an autosomal recessive defect in the alkaline phosphatase gene, ALPL.
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PubMed journal article Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in viv were found in PRIME PubMed. Download Prime PubMed App to iPhone, iPad, or Android
Interleukin-10 receptor (IL-10R) expression in human, dental pulp, fibroblast cultures was investigated by using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. After exposure to lipopolysaccharide (LPS) from Prevotella intermedia, the IL-10R mRNA levels increased after 4 h, peaked at 7 h, and dropped back to the unstimulated level at 24 h. Maximal production of the IL-10R protein in dental pulp fibroblast cultures was detected by Western blot analysis after 12 h of LPS stimulation. In contrast, the human skin fibroblast (SF-MA) and human monocyte (U937) cell lines expressed IL-10R mRNA. Anti-CD14 antibodies inhibited P. intermedia LPS-induced IL-10R mRNA expression. These results indicate that P. intermedia LPS induces IL-10R gene expression in human, dental pulp fibroblasts in vitro ...
Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix phosphoprotein that can bind calcium. DMP1 is required for bone and dentin mineralization and is expressed in the cells of bone and teeth. It is thought to play a role in regulating expression of osteoblast-specific genes during osteoblast cell differentiation and is localized to the nucleus in osteoblast precursor cells. In mature osteoblasts, the DMP1 protein is phosphorylated and localized to the extracellular matrix, where it plays a role in forming mineralized matrix. While other constitutively expressed proteins in the extracellular matrix, such as osteopontin and osteocalcin, are expressed in osteoblast cells, DMP1 is expressed in osteocytes, making it a candidate biomarker for osteocyte activity.. ...
Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix phosphoprotein that can bind calcium. DMP1 is required for bone and dentin mineralization and is expressed in the cells of bone and teeth. It is thought to play a role in regulating expression of osteoblast-specific genes during osteoblast cell differentiation and is localized to the nucleus in osteoblast precursor cells. In mature osteoblasts, the DMP1 protein is phosphorylated and localized to the extracellular matrix, where it plays a role in forming mineralized matrix. While other constitutively expressed proteins in the extracellular matrix, such as osteopontin and osteocalcin, are expressed in osteoblast cells, DMP1 is expressed in osteocytes, making it a candidate biomarker for osteocyte activity.. ...