Autophagy is a survival mechanism against stress It involves the degradation of cellular components to generate energy through the lysosome However autophagy overactivation under continuous stress leads to cell apoptosis Recombinant thrombomodulin protects vascular endothelial cells and reduces the risk of atherosclerosis Treatment with recombinant thrombomodulin increases endothelial cells Akt activation which also regulates autophagy initiation We hypothesized that recombinant thrombomodulin might influence vascular endothelial cell behaviors through regulating autophagy First we observed the expression of autophagy related proteins in endothelial cells under starvation with and without recombinant thrombomodulin treatment In starvation we found that autophagy initiation signal was induced and LC3-II and Atg5 expression contributing to autophagosomal formation increased After treating recombinant thrombomodulin Akt and mTOR was activated and autophagy-related proteins expression were inhibited ...

TY - JOUR. T1 - The inhibition in tumor necrosis factor-α-induced attenuation in endothelial thrombomodulin expression by carvedilol is mediated by nuclear factor-κB and reactive oxygen species. AU - Lin, Pen-Yuan. AU - Shen, Hsi Che. AU - Chen, Chien Jen. AU - Wu, Shu En. AU - Kao, Hsien Li. AU - Huang, Jen-Hung. AU - Wang, Danny Ling. AU - Chen, Shih-Chung. PY - 2010/1. Y1 - 2010/1. N2 - Carvedilol, a nonselective β-adrenoceptor antagonist, has been shown to possess antioxidant effects and reduce the risk of hospitalization and death in patients with severe congestive heart failure, which is featured by the activation of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), and leads to thrombotic complications. Thrombomodulin (TM) plays protective roles against thrombosis. Treatment of ECs with TNF-α resulted in a down-regulation in the TM expression in a time-dependent manner. Pre-treatment of ECs with carvedilol (1 and 10 μM) for 1 h significantly up-regulated the TM ...

Effect of Dengue Hemorrhagic Fever on Thrombomodulin Level - Free download as PDF File (.pdf), Text File (.txt) or read online for free. Effect of Dengue Hemorrhagic Fever on Thrombomodulin Level

TY - JOUR. T1 - Soluble thrombomodulin protects ischemic kidneys. AU - Sharfuddin, Asif A.. AU - Sandoval, Ruben M.. AU - Berg, David T.. AU - McDougal, Grant E.. AU - Campos, Silvia B.. AU - Phillips, Carrie L.. AU - Jones, Bryan E.. AU - Gupta, Akanksha. AU - Grinnell, Brian W.. AU - Molitoris, Bruce A.. PY - 2009/3. Y1 - 2009/3. N2 - Altered coagulation and inflammation contribute to the pathogenesis of ischemic renal injury. Thrombomodulin is a necessary factor in the anticoagulant protein C pathway and has inherent anti-inflammatory properties. We studied the effect of soluble thrombomodulin (sTM) in a hypoperfusion model of ischemic kidney injury. To markedly reduce infrarenal aortic blood flow and femoral arterial pressures, we clamped the suprarenal aorta of rats, occluding them 90%, for 60 min. Reversible acute kidney injury (AKI) occurred at 24 h in rats subjected to hypoperfusion. Histologic analysis at 24 h revealed acute tubular necrosis (ATN), and intravital two-photon microscopy ...

TY - JOUR. T1 - Treatment of thrombotic microangiopathy after hematopoietic stem cell transplantation with recombinant human soluble thrombomodulin. AU - Fujiwara, Hideaki. AU - Maeda, Yoshinobu. AU - Sando, Yasuhisa. AU - Nakamura, Makoto. AU - Tani, Katsuma. AU - Ishikawa, Tatsunori. AU - Nishimori, Hisakazu. AU - Matsuoka, Ken Ichi. AU - Fujii, Nobuharu. AU - Kondo, Eisei. AU - Tanimoto, Mitsune. N1 - Publisher Copyright: © 2015 AABB. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2016/4/1. Y1 - 2016/4/1. N2 - BACKGROUND Transplant-associated thrombotic microangiopathy (TA-TMA) after hematopoietic stem cell transplantation (HSCT) remains a severe complication associated with underlying endothelial damage. TMA has a high mortality rate with no definite treatments and effective treatments are needed. STUDY DESIGN AND METHODS The study objective was to retrospectively analyze the outcome of patients receiving recombinant human soluble thrombomodulin (rTM), which has ...

Statins; a class of routinely prescribed cholesterol-lowering drugs; inhibit 3-hydroxy-3-methylglutaryl-coenzymeA reductase (HMGCR) and strongly induce endothelial thrombomodulin (TM); which is known to have anti-inflammatory; anti-coagulation; anti-oxidant; and radioprotective properties. However; high-dose toxicity limits the clinical use of statins. The vitamin E family member gamma-tocotrienol (GT3) also suppresses HMGCR activity and induces TM expression without causing significant adverse side effects; even at high concentrations. To investigate the synergistic effect of statins and GT3 on TM; a low dose of atorvastatin and GT3 was used to treat human primary endothelial cells. Protein-level TM expression was measured by flow cytometry. TM functional activity was determined by activated protein C (APC) generation assay. Expression of Kruppel-like factor 2 (KLF2), one of the key transcription factors of TM, was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). TM

240Gly Ala Trp Asp Cys Ser Val Glu Asn Gly Gly Cys Glu His Ala Cys245 250 255Asn Ala Ile Pro Gly Ala Pro Arg Cys Gln Cys Pro Ala Gly Ala Ala260 265 270Leu Gln Ala Asp Gly Arg Ser Cys Thr Ala Ser Ala Thr Gln Ser Cys275 280 285Asn Asp Leu Cys Glu His Phe Cys Val Pro Asn Pro Asp Gln Pro Gly290 295 300Ser Tyr Ser Cys Met Cys Glu Thr Gly Tyr Arg Leu Ala Ala Asp Gln305 310 315 320His Arg Cys Glu Asp Val Asp Asp Cys Ile Leu Glu Pro Ser Pro Cys325 330 335Pro Gln Arg Cys Val Asn Thr Gln Gly Gly Phe Glu Cys His Cys Tyr340 345 350Pro Asn Tyr Asp Leu Val Asp Gly Glu Cys Val Glu Pro Val Asp Pro355 360 365Cys Phe Arg Ala Asn Cys Glu Tyr Gln Cys Gln Pro Leu Asn Gln Thr370 375 380Ser Tyr Leu Cys Val Cys Ala Glu Gly Phe Ala Pro Ile Pro His Glu385 390 395 400Pro His Arg Cys Gln Met Phe Cys Asn Gln Thr Ala Cys Pro Ala Asp405 410 415Cys Asp Pro Asn Thr Gln Ala Ser Cys Glu Cys Pro Glu Gly Tyr Ile420 425 430Leu Asp Asp Gly Phe Ile Cys Thr Asp Ile Asp Glu Cys Glu Asn Gly435 440 445Gly Phe Cys Ser Gly Val Cys His Asn ...

We found 8 novel sequence variations in the TM promoter of patients with venous thromboembolism. Six of the novel sequence variations were rare: −1848 [−1851] C/G, −1799 [−1802] G/C, −1166 [−1169] G/A, −1084 [−1087] C/T, −1078/−1079 [−1081/−1082] del CC, and −797 [−800] C/G. Among them, the −1166 [−1169] G/A mutation modified the core binding sequence (GAGACC) of a putative SSRE.20 Recent studies have shown that blood flow and the associated fluid shear stress cause morphological and functional changes in endothelial cells, including up- or downregulation of TM expression,23 24 25 which could involve this SSRE. By impairing this action, the mutation might alter endothelial cell anticoagulant functions and create a thrombotic tendency. However, we found no relationship between this mutation and the risk of thrombosis in the entire PATHROS population (327 patients and 398 controls).. The other 2 novel mutations, −1748 [−1751] G/C and −1208/−1209 ...

The protein C pathway serves many roles, probably the primary role being to work to generate activated protein C (APC) that in turn inactivates factors Va and VIIIa to inhibit coagulation. The importance of this anticoagulant activity is readily apparent because patients born without protein C die in infancy of massive thrombotic complication (purport fulminans) unless provided a protein concentrate.. The protein C anticoagulant pathway serves to alter the function of thrombin, converting it from a procoagulant enzyme into the initiator of an anticoagulant response. This occurs when thrombin binds to thrombomodulin, a proteoglycan receptor primarily on the surface of the endothelium. Thrombomodulin binds thrombin with high affinity, depending on the posttranslational modifications of thrombomodulin. Binding thrombin to thrombomodulin blocks most of thrombins procoagulant activity such as the ability to clot fibrinogen, activate platelets, and activate factor V but does not prevent thrombin ...

The protein C pathway serves many roles, probably the primary role being to work to generate activated protein C (APC) that in turn inactivates factors Va and VIIIa to inhibit coagulation. The importance of this anticoagulant activity is readily apparent because patients born without protein C die in infancy of massive thrombotic complication (purport fulminans) unless provided a protein concentrate.. The protein C anticoagulant pathway serves to alter the function of thrombin, converting it from a procoagulant enzyme into the initiator of an anticoagulant response. This occurs when thrombin binds to thrombomodulin, a proteoglycan receptor primarily on the surface of the endothelium. Thrombomodulin binds thrombin with high affinity, depending on the posttranslational modifications of thrombomodulin. Binding thrombin to thrombomodulin blocks most of thrombins procoagulant activity such as the ability to clot fibrinogen, activate platelets, and activate factor V but does not prevent thrombin ...

CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 is a C-type lectin transmembrane receptor which plays a role not only in cell-cell adhesion processes but also in host defense. CD93 belongs to the Group XIV C-Type lectin family, a group containing two other members, endosialin (CD248) and thrombomodulin, a well characterized anticoagulant. All of them contain a C-type lectin domain, a series of epidermal growth factor like domains, a highly glycosylated mucin-like domain, a unique transmembrane domain and a short cytoplasmic tail. Due to their strong homology and their close proximity on chromosome 20, CD93 has been suggested to have arisen from the thrombomodulin gene through a duplication event. CD93 was originally identified in mice as an early B cell marker through the use of AA4.1 monoclonal antibody. Then this molecule was shown to be expressed on an early population of hematopoietic stem cells, which give rise to the entire spectrum of ...

Brilliant Violet 605™ anti-human CD141 (Thrombomodulin) Antibody - CD141 is a 75 kD, single chain, type I membrane glycoprotein also known as thrombomodulin, TM, THRM, THBD, and fetomodulin.

Thrombomodulin (TM), a transmembrane glycoprotein highly expressed in endothelial cells (ECs), is a potent anticoagulant maintaining circulation homeostasis. Under inflammatory states, TM expression is drastically reduced in ECs while vascular smooth muscle cells (VSMCs) show a robust expression of TM. The functional role of TM in VSMCs remains elusive. We examined the role of TM in VSMCs activities in human aortic VSMCs stimulated with platelet-derived growth factor-BB (PDGF-BB). Using rat embryonic aorta-derived A7r5 VSMCs which do not express TM, the role of the chondroitin sulfate (CS) moiety of TM in VSMCs was delineated with cells expressing wild-type TM and the CS-devoid TM mutant. Expression of TM enhanced cell migration and adhesion/spreading onto type I collagen, but had no effect on cell proliferation. Knocking down TM with short hairpin RNA reduced PDGF-stimulated adhesion and migration of human aortic VSMCs. In A7r5 cells, TM-mediated cell adhesion was eradicated by pretreatment with

臺大位居世界頂尖大學之列，為永久珍藏及向國際展現本校豐碩的研究成果及學術能量，圖書館整合機構典藏（NTUR）與學術庫（AH）不同功能平台，成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of NTU Repository with Academic Hub to form NTU Scholars.. ...

Background: Currently there is no approved anticoagulant for treating acute stroke. This is largely because of concern for hemorrhagic complications, and suggests a critical need for safer anticoagulants. Solulin is a soluble analog of the endothelial cell receptor thrombomodulin, able to bind free thrombin and convert it to an activator of the anticoagulant, protein C. Objective: Solulin was tested for its ability to inhibit middle cerebral artery occlusion (MCAO) induced by photothrombosis, and to restore MCA patency after establishment of stable occlusion. Methods: Cerebral blood flow (CBF) was monitored by laser Doppler for 1.5 h after occlusion and again 72 h later. Results: Solulin treatment 30 min before thrombosis resulted in an approximately 50% increase in time to form a stable occlusion. When administered 30 or 60 min after MCAO, Solulin significantly improved CBF within 90 min of treatment. In contrast, none of the vehicle-treated mice showed restoration of CBF in the first 90 min ...

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THBD [ENSP00000366307]. Thrombomodulin; Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated; C-type lectin domain containing. Synonyms: THBD, P07204, P07204p, hP07204, THRM .... Linkouts: STRING Pharos UniProt OMIM ...

Research Topics, Species, Genomes and Genes, Research Grants, Publications about Recombinant and Chemo-/Bio-Orthogonal Synthesis of Liposomal Thrombomodulin

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The present invention relates to a composition comprising factor VII or a factor VII-related polypeptide and thrombomodulin or a thrombomodulin-related polypeptide, and the use thereof for treating bl

Gene identification studies, centred on a region of overlapping loss of heterozygosity in breast tumours within band 1p31.1, lead to the characterisation of LPHH1, a novel human 7TM gene. The coding sequence of LPHH1 extends over a 60 kb region and comprises in excess of 28 exons. Alternative splicing occurs minimally at five positions, four of which are within the coding sequence. The fifth region of alternative splicing occurs at the extreme 5 end of the transcript. A clear tissue specific bias in alternative exon selection is observed to some degree at all five positions, including the extreme 5 region, which raises the possibility of multiple and perhaps tissue specific promoters. One such putative promoter region, which appears to be utilised predominantly in breast cancer cells, has been identified. LPHH1 is highly evolutionarily conserved, with the simplest (19 exon) gene product being 95% identical between human and rat. Comparison of the alternatively spliced exons between three ...

SUMMARY, EXPLANATION AND LIMITATIONS:. Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.. Clone: 141C01.. Isotype: IgG1/kappa.. Immunogen: Recombinant protein encoding the six repeated EGF domains of human thrombomodulin.. Staining pattern: Cell membrane.. Positive control: Tissue sample from mesothelioma, lung or placenta.. APPLICATIONS:. This antibody is designed for the specific localization of human CD141 using IHC ...

The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008 ...

The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...

Sigma-Aldrich offers abstracts and full-text articles by [Tsung-Lin Cheng, Chao-Han Lai, Po-Ku Chen, Chia-Fong Cho, Yun-Yan Hsu, Kuan-Chieh Wang, Wei-Ling Lin, Bi-Ing Chang, Shi-Kai Liu, Yu-Ting Wu, Chao-Kai Hsu, Guey-Yueh Shi, Hua-Lin Wu].

Alanine-Scanning Mutagenesis of the Epidermal Growth Factor-Like Domains of Human Thrombomodulin Identifies Critical Residues for its Cofactor Activity. Nagashima, M.,Lundh, E.,Leonard, J.C. et al. ...

Rabbit polyclonal antibody raised against recombinant TM7SF2. Recombinant protein corresponding to amino acids of human TM7SF2. (PAB22480) - Products - Abnova

Endosialin is a protein that in humans is encoded by the CD248 gene.[1][2][3] Endosialin is a member of the Group XIV, a novel family of C-type lectin transmembrane receptors which play a role not only in cell-cell adhesion processes but also in host defence. This family comprise two other members, CD93 and Thrombomodulin which are better characterized. The function of endosialin remains elusive, but its expression has been associated with angiogenesis in the embryo and uterus and in tumor development and growth.[4] ...

Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.

Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.

ABCC5,ACP5,ADAM17,ADM,ALDH2,BCR,C5AR1,CASP1,CCL2,CCL5,CD86,CNP,CNR1,CPS1,CXCL1,CXCL10,CYP1A2,EDN1,F2R,FMO1,FOS,GJA1,IGF1,IL10,IL18BP,IL1B,JAK2,KCNJ8,LBP,LY96,MMP9,NFKBIA,NOCT,NOS2,NOS3,PCK1,PCK2,PLA2G4A,PTAFR,PTGER2,PTGS2,RELA,S100A9,SOCS1,SPARC,SRR,THBD,TLR4,TNF, ...

Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with six tandemly repeated EGF-like domains, a Serine/Threonine-rich spacer and a transmembrane domain. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI, aka carboxypeptidase B2) ...

Looking for online definition of thrombomodulin in the Medical Dictionary? thrombomodulin explanation free. What is thrombomodulin? Meaning of thrombomodulin medical term. What does thrombomodulin mean?

TY - JOUR. T1 - C-reactive protein decreases expression of thrombomodulin and endothelial protein C receptor in human endothelial cells. AU - Nan, Bicheng. AU - Yang, Hui. AU - Yan, Shaoyu. AU - Lin, Peter H.. AU - Lumsden, Alan B.. AU - Yao, Qizhi. AU - Chen, Changyi. PY - 2005/8. Y1 - 2005/8. N2 - Background. C-reactive protein (CRP) is associated with atherosclerosis and thrombosis. However, it is unclear whether CRP has direct effects on the antithrombogenic properties of endothelial cells. The objective of the present study was to determine the effect of CRP on the expression of thrombomodulin (TM) and the endothelial protein C receptor (EPCR) in human endothelial cells. Methods. Human coronary artery endothelial cells (HCAECs) were treated with CRP in a dose- and time-dependent manner. The messenger RNA levels of TM and EPCR were determined by real-time polymerase chain reaction. Anti-CD32 antibody and curcumin were used to block the potential effects of CRP. Results. In HCAECs, CRP (10 ...

Background: We evaluated associations among coagulation-related variables, resolution of disseminated intravascular coagulation (DIC), and mortality in patients with sepsis-induced DIC treated with recombinant human soluble thrombomodulin (rTM). Methods...

TY - JOUR. T1 - The histone methyltransferase SETD1A regulates thrombomodulin transcription in vascular endothelial cells. AU - Li, Zilong. AU - Chen, Baoyu. AU - Weng, Xinyu. AU - Yu, Liming. AU - Song, Mingzi. AU - Fang, Mingming. AU - Guo, Junli. AU - Xu, Yong. PY - 2018/8. Y1 - 2018/8. N2 - Thrombomodulin (TM, encoded by the THBD gene) expressed in vascular endothelial cells plays pivotal roles maintaining the equilibrium of coagulation and anti-coagulation. TM levels can be regulated at the transcriptional level although the epigenetic mechanism is underexplored. Here we report that transcriptional activation of TM in both immortalized vascular endothelial cells (EAhy926) and primary human aortic endothelial cells (HAEC) by all-trans retinoic acid (RA) paralleled accumulation of trimethylated histone H3K4, a prominent marker for active chromatin, surrounding the THBD promoter. RA treatment up-regulated the expression of SETD1A (SET1), a dedicated H3K4 methyltransferase, and augmented SETD1A ...

OBJECTIVE: The endothelial protein C-receptor (EPCR) is an endothelial transmembrane protein that binds protein C and activated protein C (APC) with equal affinity, thereby facilitating APC formation. APC has anticoagulant, antiapoptotic and antiinflammatory properties. Soluble EPCR, released by the endothelium, may bind activated neutrophils, thereby modulating cell adhesion. EPCR is therefore considered as a possible link between the anticoagulant properties of protein C and the inflammatory response of neutrophils. In the present study, we aimed to provide proof of concept for a direct binding of EPCR to the β2-integrin Mac-1 on monocytic cells under static and physiological flow conditions. MEASUREMENTS AND MAIN RESULTS: Under static conditions, human monocytes bind soluble EPCR in a concentration dependent manner, as demonstrated by flow cytometry. Binding can be inhibited by specific antibodies (anti-EPCR and anti-Mac-1). Specific binding was confirmed by a static adhesion assay, where a ...

Fingerprint Dive into the research topics of Thrombin induces thrombomodulin mRNA expression via the proteolytically activated thrombin receptor in cultured bovine smooth muscle cells. Together they form a unique fingerprint. ...

For the measurement of proCPU concentrations in human plasma, both functional and immunological assays are available.6,7,15,29⇓⇓⇓ The major advantage of immunological assays is that no activation of the zymogen is required, which makes ELISAs easy to perform. However, studies investigating a possible relationship between proCPU levels and cardiovascular diseases revealed conflicting results. ProCPU levels above the 90th percentile of population-based controls increased the risk for venous thrombosis compared with proCPU levels below the 90th percentile.30 In a case-control study of patients with stable angina pectoris, the plasma proCPU level in patients was significantly higher than in controls.31 In contrast, another study concluded that high levels of proCPU (above the 90th percentile) are protective for myocardial infarction.23. In this study, we describe the development of 2 ELISAs (T12D11/T28G6-HRP and T32F6/T9G12-HRP) for measurement of proCPU in plasma. When the antigen values ...

Endothelial dysfunction contributes to the development of acute kidney injury (AKI) in animal models of ischemia reperfusion injury and sepsis. There are limited data on markers of endothelial dysfunction in human AKI. We hypothesized that Protein C (PC) and soluble thrombomodulin (sTM) levels could predict AKI. We conducted a multicenter prospective study in 80 patients to assess the relationship of PC and sTM levels to AKI, defined by the AKIN creatinine (AKI Scr) and urine output criteria (AKI UO). We measured marker levels for up to 10 days from intensive care unit admission. We used area under the curve (AUC) and time-dependent multivariable Cox proportional hazard model to predict AKI and logistic regression to predict mortality/non-renal recovery. Protein C and sTM were not different in patients with AKI UO only versus no AKI. On intensive care unit admission, as PC levels are usually lower with AKI Scr, the AUC to predict the absence of AKI was 0.63 (95%CI 0.44-0.78). The AUC using log10 ...

Endothelial dysfunction contributes to the development of acute kidney injury (AKI) in animal models of ischemia reperfusion injury and sepsis. There are limited data on markers of endothelial dysfunction in human AKI. We hypothesized that Protein C (PC) and soluble thrombomodulin (sTM) levels could predict AKI. We conducted a multicenter prospective study in 80 patients to assess the relationship of PC and sTM levels to AKI, defined by the AKIN creatinine (AKI Scr) and urine output criteria (AKI UO). We measured marker levels for up to 10 days from intensive care unit admission. We used area under the curve (AUC) and time-dependent multivariable Cox proportional hazard model to predict AKI and logistic regression to predict mortality/non-renal recovery. Protein C and sTM were not different in patients with AKI UO only versus no AKI. On intensive care unit admission, as PC levels are usually lower with AKI Scr, the AUC to predict the absence of AKI was 0.63 (95%CI 0.44-0.78). The AUC using log10 ...

article{cc46aab3-9097-4281-9882-554963bfe958, abstract = {Protein C (PC), a 62 kDa multi-modular zymogen, is activated to an anticoagulant serine protease (activated PC or APC) by thrombin bound to thrombomodulin on the surface of endothelial cells. PC/APC interacts with many proteins and the characterisation of these interactions is not trivial. However, molecular modelling methods help to study these complex biological processes and provide basis for rational experimental design and interpretation of the results. PC/APC consists of a Gla domain followed by two EGF modules and a serine protease domain. In this report, we present two structural models for full-length APC and two equivalent models for full-length PC, based on the X-ray structures of Gla-domainless APC and of known serine protease zymogens. The overall elongated shape of the models is further cross-validated using size exclusion chromatography which allows evaluation of the Stokes radius (rs for PC = 33.15 A; rs for APC = 34.19 ...

Chung-Sheng Shi, Guey-Yueh Shi, Hsi-Min Hsiao, Yuan-Chung Kao, Kuan-Lin Kuo, Chih-Yuan Ma, Cheng-Hsiang Kuo, Bi-Ing Chang, Chuan-Fa Chang, Chun-Hung Lin, Chi-Huey Wong, Hua-Lin Wu

PURPOSE OF REVIEW: This review describes the most recent progress in xeno lung transplantation (XLTx) to date. It describes the potential mechanisms of early xeno lung graft loss, as well as the latest therapeutic strategies to overcome them. RECENT FINDINGS: Using ex-vivo perfusion models of porcine lungs with human blood, the use of genetically modified pig lungs along with novel pharmaceutical approaches has recently been studied. Strategies that have demonstrated improved lung survival include the knockout of known xenoantigens (GalTKO and N-glycolylneuraminic acid-KO), genes that regulate complement activation (hCD46 and hCD55), as well as the inflammation/coagulation cascade (human leukocyte antigen-E, human thrombomodulin, human endothelial protein C receptor, hCD47, hCD39, hCD73 and heme oxygenase-1 ...

The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008 ...

First we focused on the function of the TMD23 portion of rTM used for treatment. Previously, domain 23 has been considered an angiogenic factor,30 promoting mouse cutaneous wound healing through modulating angiogenesis at the wound site.38,39 Thus, to determine whether the anti-inflammatory effects we observed were because of a possible angiogenic effect of rTM containing domains 23, classic angiogenic molecule VEGF, and its R1 and R2 receptor proteins, were measured in the infected cornea in rTM-treated and control mice. The VEGF protein did not differ at either 3 or 5 days p.i., while the level of R1 was slightly elevated at 3 and reduced at 5 days; R2 levels were unchanged at 3 and reduced at 5 days p.i. after rTM treatment. Because numerous molecules are angiogenic and it would be impossible to test all of them, India ink35,36 was used to globally assess the angiogenic potential of the 23 domain contained in rTM. Visual, followed by statistical analysis of these data showed no difference ...

Stability of Protein-Bound Conformations of Bioactive Peptides: The Folded Conformation of an Epidermal Growth Factor-Like Thrombomodulin Fragment is Similar to that Recognize by Thrombin

Some EGF (epidermal growth factor)-type domains in extracellular proteins have conserved ASP/ASN residues that are substrates of an endoplasmic reticulum membrane-bound enzyme aspartyl/asparaginyl beta-hydroxylase (EC 1.14.11.16). The ASX residues are hydroxylated by the enzyme, forming the beta hydroxylated residue. Many blood coagulation factors, the LDL receptor, BMP-1, Thrombomodulin, and EGF have the modification. Drosophila proteins involved in development include crumbs, delta, notch, serrate, slit and tolloid. May be restricted to metazoans. The Prosite entry PS00010 lists many modified proteins ...

It has a papillary or tubulopapillary pattern with a single layer of atypical mesothelium overlying a fibrovascular core. The tumor cells are typically epithelioid, not spindled. Stromal invasion is present, and psammoma bodies are variable. Mesotheliomas of the testis, as at other sites, are immunoreactive for calretinin, EMA, thrombomodulin and CK7, and negative for CK20 and CEA (Am J Surg Pathol 2006;30:1 ...

Mouse monoclonal Thrombomodulin antibody [Phx-01] validated for WB, ELISA and tested in Human. Immunogen corresponding to recombinant full length protein

Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.

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