3VSV: The substrate/product-binding modes of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485
With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20-31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenting organisms to produce acetate at the expense of ethanol. Hydrogen and ethanol are both more reduced than glucose. Thus there is a tradeoff between production of these compounds imposed by electron balancing requirements; however, the mechanism is not fully known. Deletion of the hfsA or B subunits resulted in a roughly 1.8-fold increase in ethanol yield. The increase in ethanol production appears to be associated with an increase in alcohol dehydrogenase activity, which appears to be due, at least in part, to increased expression of the adhE gene, and may suggest a regulatory linkage between hfsB and adhE. We studied this system most intensively in the organism Thermoanaerobacterium saccharolyticum; however, deletion of hfsB also increases ethanol production in other thermophilic bacteria
CBM16 is a type B CBM family, with a characteristic concave cleft, allowing the binding of substrate longer than triose. The ligand binding cleft shows some promiscuity as it can accommodate both pentoses (glucose and mannose), but only in the context of planar polymer like β-1,4-glucans, and not helical β-1,3-glucans [1]. The crystallographic structure determination of both CBM of Caldanaerobius polysaccharolyticus (formerly Thermoanaerobacterium polysaccharolyticum) ManA revealed the importance of two aromatic residues in the binding cleft, as long as two stretches of polar residues on both sides of the cleft [1]. Study of affinity of targeted mutant for the predicted key resides confirmed the importance of two tryptophanes (Trp-20 and Trp-125), and two glutamines (Gln-81 and Gln-93) [2]. Based on sequence similarity and conservation of secondary structure element, it has been proposed that along with the CBM-4, 17, 22 and 27 families, they form a superfamily [9]. ...
CBM16 is a type B CBM family, with a characteristic concave cleft, allowing the binding of substrate longer than triose. The ligand binding cleft shows some promiscuity as it can accommodate both pentoses (glucose and mannose), but only in the context of planar polymer like β-1,4-glucans, and not helical β-1,3-glucans [1]. The crystallographic structure determination of both CBM of Caldanaerobius polysaccharolyticus (formerly Thermoanaerobacterium polysaccharolyticum) ManA revealed the importance of two aromatic residues in the binding cleft, as long as two stretches of polar residues on both sides of the cleft [1]. Study of affinity of targeted mutant for the predicted key resides confirmed the importance of two tryptophanes (Trp-20 and Trp-125), and two glutamines (Gln-81 and Gln-93) [2]. Based on sequence similarity and conservation of secondary structure element, it has been proposed that along with the CBM-4, 17, 22 and 27 families, they form a superfamily [9]. ...
The demand for cellulosic biofuels is on the rise because of the anticipation for sustainable energy and less greenhouse gas emissions in the future. However, production of cellulosic biofuels, especially cellulosic butanol, has been hampered by the lack of potent microbes that are capable of converting cellulosic biomass into biofuels. We report a wild-type Thermoanaerobacterium thermosaccharolyticum strain TG57, which is capable of using microcrystalline cellulose directly to produce butanol (1.93 g/liter) as the only final product (without any acetone or ethanol produced), comparable to that of engineered microbes thus far. Strain TG57 exhibits significant advances including unique genes responsible for a new butyrate synthesis pathway, no carbon catabolite repression, and the absence of genes responsible for acetone synthesis (which is observed as the main by-product in most Clostridium strains known today). Furthermore, the use of glucose analog 2-deoxyglucose posed a selection pressure to ...
Metabolic Engineering of Thermophilic Bacillus licheniformis for Chiral Pure D-2,3-Butanediol Production Another BDO, 2,3-Butanediol, is also a potential fuel and a platform chemical. Organisms that natively produce the chemical are pathogenic and can only form the product with fermentations at 37°C. To transform the Bacillus licheniformis they had to use a protoplast fusion method. They were able to utilize xylose as a feedstock at 50°C to create 2,3-Butanediol. This will be helpful to utilize lignocellulose substrates as higher temperatures are helpful because of higher rates of degradation and fewer enzymes are need to be added [13]. Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield In this paper the authors used Thermoanaerobacterium saccharolyticum and made knockouts in the genes for acetate kinase, phosphate acetyltransferase, and L-lactate dehydrogenase. Their strain was able to produce high yields of ethanol as the only measurable fermentation ...
The enzyme, isolated from the thermophilic bacterium Thermoanaerobacterium thermosaccharolyticum, catalyzes the reversible phosphorolysis of sucrose 6(F)-phosphate ...
The first crystal structures of a two-domain, prokaryotic glucoamylase were determined to high resolution from the clostridial species Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eukaryotic glucoamylases. Interdomain contacts are common to all prokaryotic Family GH15 proteins. Domains similar to those of prokaryotic glucoamylases in maltose phosphorylases (Family GH65) and glycoaminoglycan lyases (Family PL8) suggest evolution from a common ancestor. Eukaryotic glucoamylases may have evolved from prokaryotic glucoamylases by the substitution of the N-terminal domain with the peripheral subdomain and by the addition of a starch-binding domain. Crystal structure and evolution of a prokaryotic glucoamylase.,Aleshin AE, Feng PH, Honzatko RB, Reilly PJ J Mol Biol. 2003 Mar ...
Numerous significant hits using gapped BLAST to naphthoate synthase from H. parahaemolyticus (1086070), H. influenzae (1170915), E. coli (126968), Synechocystis sp. (7428382), among others. Also many hits to putative enoyl-CoA hydratase/isomerase from Arabidopsis thaliana (2462733), Archaeoglobus fulgidus (7428375), and to crotonase from Thermoanaerobacterium thermosaccharolyticum (1903327), among others ...
Cell-associated oligo-1,6-alpha-glucosidase (EC 3.2.1.10) was isolated from Thermoanaerobium Tok6-B1 grown on starch-containing medium. Activity was purified 11.4-fold by salt precipitation, gel filtration, hydroxyapatite and anion-exchange chromatography. Molecular mass was determined as 30,000 by SDS/polyacrylamide-gel electrophoresis and 33,000 by analytical gel filtration. The probable order of specificity was p-nitrophenyl-alpha D-glucose greater than-isomaltose greater than-isomaltotriose greater than-panose greater than-nigerose and no activity was shown against malto-oligosaccharides, melezitose, melibiose, raffinose, cellobiose, sophorose, gentiobiose, lactose, pullulan, dextran or amylose. The optima for activity and stability were between pH 5.6 and 7.0 and the half-life at pH 6.5 was 1000 min at 70 degrees C and 20 min at 76 degrees C. Activity was stabilized by substrate, Mg2+, Mn2+ and Ca2+, but was destabilized by Zn2+ and EDTA. N-Ethylmaleimide, glucose and 1-O-methyl-alpha ...
As a renewable and clean energy carrier, the production of biohydrogen from low-value feedstock such as lignocellulose has increasingly garnered interest. The NADH-dependent reduced ferredoxin:NADP+ oxidoreductase (NfnAB) complex catalyzes electron transfer between reduced ferredoxin and NAD(P)+, which is critical for production of NAD(P)H-dependent products such as hydrogen and ethanol. In this study, the effects on end-product formation of deletion of nfnAB from Thermoanaerobacterium aotearoense SCUT27 were investigated. Compared with the parental strain, the NADH/NAD+ ratio in the ∆nfnAB mutant was increased. The concentration of hydrogen and ethanol produced increased by (41.1 ± 2.37)% (p | 0.01) and (13.24 ± 1.12)% (p | 0.01), respectively, while the lactic acid concentration decreased by (11.88 ± 0.96)% (p | 0.01) when the ∆nfnAB mutant used glucose as sole carbon source. No obvious inhibition effect was observed for either SCUT27 or SCUT27/∆nfnAB when six types of lignocellulose
Caldanaerobacter subterraneus subsp. subterraneus ATCC ® BAA-225™ Designation: SEBR 7858 TypeStrain=True Application: Biotechnology
jw.org կայքի բժշկական բաժինը տեղեկություն է տրամադրում բժիշկներին և առողջապահության ոլորտի մասնագետներին։ Այս բաժինը չի առաջարկում բուժման որևէ մեթոդ կամ տալիս խորհուրդներ։ Այն նաև չի փոխարինում առողջապահության ոլորտի մասնագիտական որակավորում ունեցող բժշկին։ Թեև բերված բժշկական գրականությունը Եհովայի վկաները չեն հրատարակել, բայց դրանցում խոսվում է առանց արյան փոխներարկման ստրատեգիաների մասին, որոնք կարելի է հաշվի առնել։ Յուրաքանչյուր մասնագետի պատասխանատվություն է իրազեկ լինել բժշկության ոլորտում վերջին ձեռքբերումներին, պացիենտների հետ ...
and are thermophilic bacteria that have been engineered to produce ethanol from the cellulose and hemicellulose fractions of biomass, respectively. from mostly NADH linked to mostly NADPH linked resulted in higher ethanol production by and in particular, hold great promise for the production of ethanol Simeprevir from lignocellulosic feedstocks (1, 2). is a thermophilic, Gram-positive obligate anaerobe that rapidly consumes cellulose. Engineered strains of (3), a thermophilic anaerobe that ferments xylan Simeprevir and Simeprevir other sugars derived from biomass, have been shown to produce ethanol at >50 g/liter, a near-theoretical yield (4). While comparable concentrations of ethanol are tolerated by selected strains of (5,C7), Rabbit Polyclonal to MP68 the maximum reported concentration of ethanol produced by this organism is 23.6 g/liter (8) and the maximum ethanol yield achieved to date is 51% of the theoretical maximum (9) versus 92% in (10). It is of interest to understand why ethanol ...
A Novel GH7 Endo-β-1,4-Glucanase from Neosartorya fischeri P1 with Good Thermostability, Broad Substrate Specificity and Potential Application in the Brewing Industry. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
DNA mismatch repair (MMR) is a highly conserved biological pathway that plays a key role in maintaining genomic stability. MMR corrects DNA mismatches generated during DNA replication, thereby preventing mutations from becoming permanent in dividing cells. MMR also suppresses homologous recombination and was recently shown to play a role in DNA damage signaling. Defects in MMR are associated with genome-wide instability, predisposition to certain types of cancer including HNPCC, resistance to certain chemotherapeutic agents, and abnormalities in meiosis and sterility in mammalian systems. The Escherichia coli MMR pathway has been extensively studied and is well characterized. In E. coli, the mismatch-activated MutS-MutL-ATP complex licenses MutH to incise the nearest unmethylated GATC sequence. UvrD and an exonuclease generate a gap. This gap is filled by pol III and DNA ligase. The GATC sites are then methylated by Dam. Several human MMR proteins have been identified based on their homology to ...
Pacific Ethanol Inc. of Sacramento has agreed to use grain sorghum - grown on up to 30,000 acres of California farmland - to produce ethanol at its plants, including one in Stockton.
1PED: Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry.
Process for production of ethanol at high efficiency, selectivity and conversion rate by the homologation reaction of methanol and carbon monoxide and hydrogen using a catalyst system containing alkali metal atom, cobalt atom, iodine atom and, optionally, ruthenium atom and an organic tertiary amino compound.
Caldicellulosiruptor kristjanssonii ATCC ® 700853™ Designation: DSM 12137 TypeStrain=True Application: Degrades cellulose Biotechnology
Gevo Inc. has released its first quarter financial results, reporting it recently closed on $25.9 million in financing and has successfully begun side-by-side operation at its Luverne, Minn., plant, with the intent to produce ethanol and isobutanol.
The chemical reaction between yeast and sugar produces ethanol and carbon dioxide. The balanced chemical equation for this reaction is glucose/sugar (C6H12O6) in the presence of the yeast enzyme...
Production of ethanol and biogas from acetic acid-impregnated steam-pretreated wheat straw was investigated. The solid fraction after pretreatment was used at high solids concentrations to generate ethanol by simultaneous saccharification and fermentation (SSF). The residual streams were evaluated with regard to biogas production. The experimental results were used to perform a techno-economic evaluation of a biorefinery concerning ethanol, raw biogas, pellet, and electricity production at various solid contents and residence times in the fermentation step. The configurations were also altered to include biogas upgrading to vehicle fuel quality or fermentation of xylose to ethanol. At a water-insoluble solids (WIS) content in the SSF of between 10 and 20 %, the ethanol yields exceeded 80 %, the highest being 86 % at 12.5 % WIS, expressed as % of the theoretical maximum, based on the glucan content in SSF. Anaerobic digestion of wheat straw hydrolysate and stillage yielded 4.8 and 1.0-1.2 g methane/100 g
A collaborative led by researchers at the University of Illinois and including the Lawrence Berkeley National Laboratory, the University of California at Berkeley, Seoul National University and BP have engineered a strain of Saccharomyces cerevisiae-the common industrial yeast-to co-ferment glucose and xylose simultaneously to produce ethanol. In a process of...
ALGAE TO ETHANOL: Using algae fermentation to produce ethanol . 4 th AFRICAN BIOFUEL CONFERENCE March 2009. Algae!. Why Algae?. Fast growers relative to other plants and animals can double their weight every day High carbohydrate/low lignin content Gallons of oil per acre per year...
Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.
When 25 mL of C2H5OH (density 0.80 g.cm-3) is dissolved in water to prepare a 250 mL solution, the density of the solution was found to be 0.950 g.cm-3. Calculate a. Molarity of pure ethanol; b. Molarity of ethanol in the final solution ...
Im pretty sure this is AutoblogGreens first story about Guinea Bissau, the small west African country. An official in that countrys Defense Ministry said
Coskata, a firm from Illinois, promises to produce ethanol for less than $1 per gallon from a process that uses bacteria for ethanol generation from organic material.
Not a bad idea if you ask me. Ive read a lot on the subject of ethanol production, and Ive heard different sides to it. Ive seen a few posts where the claim is that corn based, and especially switch grass based ethanol production actually uses more than a barrel of oil to produce a barrel of ethanol. Which makes sense if you trace it to all the farm equipment used to plant, maintain and harvest crops. So if seaweed can be used to produce ethanol, I dont see why not. Seaweed is known to grow as much as six feet a day, so it seems like an ideal crop. Also there arent the connotations of raising food prices, and all the economics behind the big corn boom. Anyways, click here for the article ...
Ethanol poisoning occurs in cats when they ingest a substance that either contains alcohol or produces ethanol inside the body after ingestion. Ethanol poisoning may cause depression of the central nervous system - Wag! (formerly Vetary)
dTDP-3-amino-2,3,6-trideoxy-4-keto-D-glucose/dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose/dTDP-2,6-dideoxy-D-kanosamine transaminase [EC:2.6.1.106 2.6.1 ...
China Complete Line to Produce Ethanol From Sugarcane Molasses Concentration of Ethanol, Find details about China Alcohol Distillery, Ethanol Distillery from Complete Line to Produce Ethanol From Sugarcane Molasses Concentration of Ethanol - Feicheng Jinta Machinery Co., Ltd.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Ethanol fermentation by yeast is a vital process during the manufacture of ethanol or alcohol and while in the means of anaerobic respiration yeast generates ethanol. Its very important to restrict the existence of oxygen during the fermentation procedure to ensure the yeast can ferment sugars into ethanol or consuming alcohol due to the fact it is also known.. Each individual sort of ethanol production which include ethanol for human intake and bio ethanol for powering engines needs to pass through the fermentation practice that converts the mashed mixture of water along with various fruits or grains or vegetables into ethanol along with the wished-for alcoholic power. After the mash is ready for sugar fermentation then dried yeast powder is additional towards the combination to start out the method of converting sugars current from the mixture into ethanol. Even so, for the duration of this method, it is rather critical to stop oxygen from remaining present in the combination since it is ...
Future corn availability: Forecasts show an anticipated increase in corn yields over the next 10 years of 17 percent, resulting in an estimated increase in Nebraska corn production from 1.27 billion bushels for the 2005-06 crop year to 1.51 billion bushels for the 2015-16 crop year. Projections of feed use for the same period show a significant decrease due to the additional availability of the distillers grains products ...
The South Bend Ethanol Plant has a new owner.. Noble Americas South Bend Ethanol has been bought by Mercuria, and has been renamed simply South Bend Ethanol.. Noble Americas reopened the plant in 2015 after it was shut down when New Energy filed for bankruptcy. The plant produces ethanol, carbon dioxide, corn oil and dry distillers grains.. Mercuria plans to spend up to $30 million to make South Bend Ethanol more efficient and reliable, and improve production by half. The company says it is committed to South Bend, and will partner with local farmers.. ...
Consider the following gasphase reaction to produce ethanol from ethylene Take the initial number of moles of ethylene to equal 1 The initial number of moles of water is determined by the selected steamtoethylene molar ratio Initially there is no ethanol in the reaction vessel This Demonstration plots the extent of reaction versus pressure for userset values of the temperature It follows from t
Environmental initiatives seem, at first blush, to be immune from the vagaries of rent-seeking but alas, they are not. I urge everyone to read Jonathan Adlers Rent Seeking Behind the Green Curtain. It was an eye-opener for me and I have assigned the reading to numerous students. Ive had some almost break down in tears during class discussion because they perceived that there was nothing that could be done about the issue, something which we will explore later on in the quarter ...
My supply of Iso-Propyl Alcohol has ran out, and I need something as a fuel and disinfectant for random experiments I do all the time, and Im not...
FROM THE JULY ISSUE: RFA caps corn ethanol at 15 billion gallons per year and we have reached and exceeded that level. Future growth must meet true demand, and that demand is blocked by unnecessary, arbitrary and baseless regulations.
I tried re-watching The Lost World a few years ago, but I bailed when I realized that was actually Vince Vaughn and not just a really annoying new...
TY - JOUR. T1 - Characterization of a multidomain cellulase from an extremely thermophilic anaerobe strain NA10. AU - Miyake, Katsuhide. AU - Machida, Yuichi. AU - Hattori, Kouji. AU - Iijima, Shinji. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 1998. Y1 - 1998. N2 - The nucleotide sequence of a β-glucanase gene from an extremely thermophilic anaerobe NA10 was determined. The open reading frame extended over 3000 bp and encoded a polypeptide with a molecular mass of 113 kDa. The deduced amino acid sequence of this protein exhibited high homology to a bifunctional cellulase CelB of Caldocellum saccharolyticum. Based on the homology to GelB, the NA10 β-glucanase appears to comprise three domains: N- terminal, central, and C-terminal domains. Among these, N- and C-terminal domains apper to be catalytic domains, and the central domain to be a cellulose binding domain. These domains were joined with each other by proline and threonine rich segments (PT box). The ...
In Zimbabwe, the countrys Energy Regulatory Authority says it has an open door policy for any would-be ethanol producer to apply for a production license in order to supply the blending mandate, contrary to popular belief that the opportunity was restricted to just a few players. Greenfuel and Triangle are the only two producers currently allowed to produce ethanol, but others are welcome to as well, such as Hippo Valley who supplies molasses to Triangle but has the capacity to produce ethanol itself. ...
The share of U.S. corn production used to produce ethanol increased from 12.4 percent in the 2004/05 crop year to over 38.5 percent in the 2010-11 crop year, and remained at that high level in 2011-12. Even after accounting for the return of by-products to the feed market, this is a large and persistent new demand for corn that surely has changed price dynamics. Nevertheless, the role of biofuels in determining recent high corn and other agricultural commodity prices, as well as their volatility, remains controversial. Policy measures to encourage biofuels production, including the Renewable Fuels Standard (RFS) mandates, subsidies to ethanol blenders, regulations on gasoline chemistry, and import tariffs, all have helped to create this new, persistent demand for corn and have contributed to incentives to create the capacity to produce ethanol and to use corn for fuel rather than food. Various aspects of implementing that policy, and the economics of plant operation, suggest a very inelastic ...
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im starting this as part of my break from hormones hoping to bulk up 10 lbs in 12 weeks. goin like this. 1-4 mass FX 4 caps ED, 5-8 6 caps ED 1-4 Jw 3
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Physiology Test Question - This method of staining nucleic acids has proven extremely useful in studying cells of the nervous system. The method is named after this scientist.
Watermelons could do more than grace the tables at picnics across the land: They could also serve as a source of biofuel. Researchers fermented watermelon juice to produce ethanol, according to a study published in Biotechnology for Biofuels, and while the melons arent likely to become a primary biofuel crop, the process could help out farmers.. Nearly one-fifth of the watermelon crop grown in the United States is left in the fields after harvest because of defects on the melons rinds. Its not that theres anything wrong with the melon on the inside, but our only method of judgment is the outside, said [lead author] Wayne Fish [Greenwire]. Although farmers often till the abandoned melons into the soil, the value of the nutrients provided by this practice is much less than the overall cost to farmers of losing so much of their crop.. Read More. ...
Background: Phenotypes exhibited by microorganisms can be useful for several purposes, e. g., ethanol as an alternate fuel. Sometimes, the target phenotype maybe required in combination with other phenotypes, in order to be useful, for e. g., an industrial process may require that the organism survive in an anaerobic, alcohol rich environment and be able to feed on both hexose and pentose sugars to produce ethanol.
If you want to avoid sugar, aspartame, trans-fats, MSG, or just about anything else, you read the label. If you want to avoid G.M.O.s - genetically modified organisms - youre out of luck. Theyre not listed. You could, until now, simply buy organic foods, which by law cant contain more than 5 percent G.M.O.s. Now, however, even that may not work.. In the last three weeks, the U.S. Department of Agriculture has approved three new kinds of genetically engineered (G.E.) foods: alfalfa (which becomes hay), a type of corn grown to produce ethanol), and sugar beets. And the approval by the Food and Drug Administration of a super-fast-growing salmon - the first genetically modified animal to be sold in the U.S., but probably not the last - may not be far behind ...