TY - JOUR. T1 - Effect of phorbol myristate acetate-induced lung injury on airway blood flow. AU - Barman, Scott A. AU - Ardell, J. L.. AU - Taylor, A. E.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - The effects of phorbol myristate acetate (PMA) induced lung injury on the pulmonary and systemic blood flow contributions to the trachea and main bronchi (upper airways) were assessed in anesthetized dogs by injecting 15 μm radiolabeled microspheres into the right and left heart, respectively. Upper airway blood flow was studied in lungs given the following treatments: (1) PMA; (2) PMA in lungs pretreated with the thromboxane synthetase inhibitor OKY-046, and (3) PMA in lungs pretreated with the antioxidant catalase. After microsphere injections, the tracheal cartilage, tracheal muscle-mucosa, and main bronchi were excised. The results of this study indicate that under normal conditions, tracheal mucosa [33-52 ml·min-·(100 g)-1] and tracheal cartilage [18-27 ml·min-1·(100 g)-1] blood flow is primarily ...
We isolated a group of genes that are rapidly and transiently induced in 3T3 cells by tetradecanoyl phorbol acetate (TPA). These genes are called TIS genes (for TPA-inducible sequences). Epidermal growth factor (EGF), fibroblast growth factor (FGF), and TPA activated TIS gene expression with similar induction kinetics. TPA pretreatment to deplete protein kinase C activity did not abolish the subsequent induction of TIS gene expression by epidermal growth factor or fibroblast growth factor; both peptide mitogens can activate TIS genes through a protein kinase C-independent pathway(s). We also analyzed TIS gene expression in three TPA-nonproliferative variants (3T3-TNR2, 3T3-TNR9, and A31T6E12A). The results indicate that (i) modulation of a TPA-responsive sodium-potassium-chloride transport system is not necessary for TIS gene induction either by TPA or by other mitogens and (ii) TIS gene induction is not sufficient to guarantee a proliferative response to mitogenic stimulation. ...
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascades involving MAPK1/3 (ERK1/2) and RAP1GAP. Depending on the cell type, is involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation. In cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 ...
Effect of PMA treatment on the expression of cyclins and cdks in IEC-18 cells. Cells were exposed to 100 nM PMA for the indicated times (U, untreated) and subje
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The present studies were undertaken to determine whether the CDKI FP could enhance PMA-induced maturation in human leukemia cells. The rationale for this investigation stemmed from several considerations: (a) FP has been shown to induce differentiation in some cell types (e.g., non-small cell lung cancer cells; Ref. 21 ); and (b) inhibition of cell cycle progression by FP might promote a leukemic cell differentiation program (47) . Contrary to expectations, coexposure to FP for 24 h strikingly opposed PMA-induced differentiation in U937 cells and instead significantly increased apoptosis. These events were associated with increased mitochondrial dysfunction, activation of caspases, and loss of clonogenic survival; moreover, enhanced cell death after PMA/FP cotreatment was also observed in promyelocytic leukemia cells (HL-60) and in U937 cells overexpressing the antiapoptotic protein Bcl-2. These events may reflect the complex reciprocal relationship that exists between differentiation and ...
Fingerprint Dive into the research topics of Enhanced vascular reactivity to protein kinase C activators in genetically hypertensive rats. Together they form a unique fingerprint. ...
Recent studies have indicated a link between levels of cyclooxygenase-2 (COX-2) and development of the multidrug resistance (MDR) phenotype. The ATP-binding cassette sub-family G member 2 (ABCG2) is a major MDR-related transporter protein that is frequently overexpressed in cancer patients. In this study, we aimed to evaluate any positive correlation between COX-2 and ABCG2 gene expression using the COX-2 inducer 12-O-tetradecanoylphorbol-13-acetate (TPA) in human breast cancer cell lines. ABCG2 mRNA and protein expression was studied using real-time RT-PCR and flow cytometry, respectively. A significant increase of COX-2 mRNA expression (up to 11-fold by 4 h) was induced by TPA in MDA-MB-231 cells, this induction effect being lower in MCF-7 cells. TPA caused a considerable increase up to 9-fold in ABCG2 mRNA expression in parental MCF-7 cells, while it caused a small enhancement in ABCG2 expression up to 67 % by 4 h followed by a time-dependent decrease in ABCG2 mRNA expression in MDA-MB-231 cells.
In the present study, ISL, a flavonoid isolated from licorice, was revealed to be a potent therapeutic agent for ACC. Although this natural flavonoid has been extensively considered as an antineoplastic agent in various human cancers (Hsu et al., 2005; Yoshida et al., 2008; Ye et al., 2009) and has shown inhibitory effects on the phorbol myristate acetate-induced angiogenic process of endothelial cells (Kang et al., 2010), this is the first study regarding prevention of tumor angiogenesis as an important component of the antitumor mechanisms of ISL. In this study, we demonstrated for the first time that ISL was a potent inhibitor of tumor-induced angiogenesis in ACC. Initially, by using growth analysis and viability measurement, we determined that ISL at a concentration of 0 to 20 μM did not induce significant ACC cell death but only effectively decreased the cell growth activity. Further investigation revealed that ISL at these concentrations not only significantly prevented ACC-mediated ...
Using oligonucleotide microarray, we have identified and cloned a novel gene that was induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in CD18 pancreatic cancer cells. In-silico analysis suggested localization of the gene product to the endoplasmic reticulum, thus we named it TPA induced Trans-Membrane Protein (TTMP). We found that TTMP is highly expressed in normal human pancreas, but has low expression in cancer cell lines. Confocal immunofluorescence microscopy localized TTMP to the endoplasmic reticulum. CD 18 and HeLa cells stably expressing TTMP inhibited cell proliferation. Conversely, siRNA duplexes targeted to TTMP in CD18 cells led to an increase in cell proliferation, as did clones expressing an in-frame N-terminal truncation of TTMP. Cell cycle analysis showed that TTMP induced a G1 phase arrest in pancreatic cancer cells. Finally, the promoter of TTMP was cloned, and basal activity was found to be dependent on Sp1 ...
Heparin is a potent inhibitor of the proliferation and migration of vascular smooth muscle cells. This agent selectively inhibits the transcription of tissue-type plasminogen activator and interstitial collagenase, probably by decreasing the binding of activator protein-1 (AP-1) to phorbol ester-responsive elements in the promoters of these genes. Decreased AP-1 binding is not due to a direct inhibition by heparin, since heparinase digestion of nuclear extracts prepared from heparin-treated smooth muscle cells does not restore AP-1 binding activity. Treatment of cells with heparin suppresses the expression of Jun B, one of the components of AP-1. The major effect of heparin is at the level of posttranslational modification of Jun B. Results from pulse-chase labeling experiments show that the newly synthesized Jun B is rapidly converted to a higher-molecular-weight form and that conversion is suppressed by heparin. Evidence is presented suggesting that the heparin-inhibited event is ...
TY - JOUR. T1 - Reversal of defective IL-6 production in lipopolysaccharide-tolerant mice by phorbol myristate acetate. AU - Mengozzi, Manuela. AU - Sironi, Marina. AU - Gadina, Massimo. AU - Ghezzi, Pietro. PY - 1991/8/1. Y1 - 1991/8/1. N2 - The development of LPS tolerance has been suggested to be mediated by an inhibition of cytokine synthesis. Here we have studied serum IL-6 and TNF levels in mice after LPS administration. Repeated administration of LPS (35 μg daily for 4 days) to mice induced a refractoriness (tolerance) to subsequent administrations of LPS in terms of induction of circulating IL-6 and TNF. To investigate the mechanism by which LPS down-regulates its own induction of cytokine synthesis and the relationship between IL-6 and TNF production, we attempted to revert the inhibition of IL-6 and TNF production using agents like PMA or IFN-γ, previously reported to activate macrophage production of cytokines. Pretreatment with PMA (4 μg, 10 min before LPS) partially restored IL-6 ...
cytosolic-nuclear tumor promoter binding protein: binds tumor promoters such as tetradecanoylphorbol-13 acetate, teleocidins, aplysiatoxin & thapsigargin; MW 70 kDa existing in cytosolic fraction as a complex with the 90 kDa heat-shock protein; natural ligand is yakkasterone; amino acid sequence has been determined
Activation of PPI hydrolysis by carbachol elicits a robust translocation of CaM from membranes into cytosol which was previously shown to be mimicked by the addition of the calcium ionophore ionomycin and the phorbol ester TPA28 ...
T cell activation via the T cell receptor (T3-Ti complex) by OKT3 results in modulation of the T3-Ti complex, but does not affect T4, T8, or T11 antigen expression. To study the effect of other T cell activators on these T cell membrane antigens, the authors incubated mononuclear cells for 0-3 days with lectins or pharmacologic agents and stained with monoclonal antibodies to their antigens. The median fluorescence intensity (MFI) was measured with a fluorescence activated cell sorter. Activation of PBL with Con A, PHA, calcium ionophore A23187, or with dbcAMP, isoproterenol, or theophyllin had minimal effects on the MFI of T3, T4, T8, or T11. Phorbol myristate acetate (PMA), a protein kinase C activator which stimulates PBL though an alternate pathway, caused a 90-100% reduction of T3 and T4 MFI, a 25% reduction in T8 MFI, and a 400% increase in T11 MFI after 2 days. Addition of A23187 slightly increased these effects. PMA induced a 2-3-fold increase in cell diameter concomitant with the ...
Carcinogenesis is caused by a cumulative, multistage process that mainly consists of initiation, promotion, and progression. ROS, which are produced as a result of the metabolism of molecular oxygen via biochemical reactions in cells, play a key role in tumor promotion. Some tumor promoters accelerate/induce the conversion of initiated cells (carcinogen-mediated mutation or potential "stem cells") into tumorigenic cells possibly via the production of oxidative/inflammatory responses (30, 31). TPA (12-O-tetradecanoylphorbol-13-acetate), a phorbol ester, is a tumor promoter that induces the neoplastic/tumorigenic transformation of preneoplastic JB6 cells through the overproduction of ROS (32). In this study, we investigated the inhibitory effect of SFN on TPA-stimulated neoplastic transformation in the mouse epidermal JB6 P+ cell line to assess whether SFN is able to block tumor promoter-induced tumorigenesis in skin cells. Our results show that SFN was effective when it was given together with ...
Topical application of marine briarane-type diterpenes effectively inhibits 12-O-tetradecanoylphorbol-13-acetate-induced inflammation and dermatitis in murine skin. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
I have started measuring in-situ Protein Kinase C activity in permeabilized cells grown in 96-well plates, based on a few references from the literature such as: Heasley L.E., J.Biol.Chem., 1989, 264, 8646. I always get high activity after stimulation with phorbol esters and very low activity after treatment with PKC inhibitors. The problem is that the activity in untreated cells is often almost as high as in stimulated cells. Any advice or protocol would be welcome. Thanks Bernard medbpl at emory.edu ...
During activation, PKC isoforms translocate to the plasma membrane (Nishizuka, 1984), and are autophosphorylated at multiple amino acid residues (Keranen et al., 1995; Newton, 2003). We attempted to measure PKC activation directly via: 1) PKCδ immunostaining (Fig. 6A), 2) membrane/cytosol fractionation and PKCδ Western blotting (Brown et al., 2005), 3) anti-phospho-PKCδ Western blotting (Sumandea et al., 2008), 4) anti-pan-phospho-PKC Western blotting (Iwabu et al., 2004), 5) a GFP biosensor based on the C1 domains of PKCγ (Oancea et al., 1998), and 6) a FRET biosensor of PKC activity (Violin et al., 2003). Unfortunately, we did not observe endogenous PKC activation with any of these techniques after stimulating endogenous M3 receptors with carbachol, and were therefore unable to directly measure the effect of DGKη on endogenous PKC activity. This did not reflect technical limitations, as we did observe endogenous PKCδ activation after direct stimulation with PMA (Fig. 6A). Instead, our ...
We have previously shown that multiple topical applications, over 11 days, of the phorbol ester 12- O-tetradecanoylphorbol-13-acetate (TPA) induces a persistent inflammatory reaction characterized by...
Viktige forbindelser for romstasjonen, slike som ulike væsker, miljøkontroll og livsstøtte-systemer, elektriske og datasystemer går igjennom Unity for å forsyne arbeids- og boligområdene på romstasjonen. Mer enn 50 000 mekaniske deler, 216 ledninger for å frakte væsker og gass og 121 interne og eksterne elektriske kabler, er installert i modulen. Unity er laget av aluminium. Før oppskyting ombord i Endeavour ble «Pressurized Mating Adapters» (PMA-1 og PMA-2) montert på koblingsmekanismene foran og bak på Unity. Disse adapterne tillater bruk av både de amerikanske romfergene og russiske moduler. PMA-1 forbinder nå permanent Unity med modulen Zarja. PMA-2 brukes for tilkobling av romfergen. Koblet til utsiden av PMA-1 er datamaskiner, eller «multiplexer-demultiplexers» (MDMs), som sørget for den tidlige kontrollen over Unity. Unity er også utstyrt med et kommunikasjonssystem som tillater data, lyd og lavhastighets data video-overføringer til Houston. Dette var ment som et ...
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I own an acetate that seems to be the same as the record you mentioned here but I got it without much information. The only thing I was told by the Italian seller is that the records came directly from the states. Im surprised to hear that your "source" says the record comes from the UK ...
Celanese Corp. (CE) and funds managed by Blackstone (BX) announced a definitive agreement to form a JV that will create a global acetate tow supplier. Celanese and Blackstone will own 70 percent and 30 percent of the JV, respectively.
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In vitro studies have shown that the phorbol ester, 12-tetradecanoylphorbol 13-acetate (TPA) induces neural crest cell differentiation into melanocytes, and stimulates proliferation and differentiation of normal melanocytes. As TPA is not a physiological agent, its action is clearly mimicking some in vivo pathway involved in these processes. An understanding of the effect of TPA on the expression of melanogenic genes will therefore provide valuable insight into the molecular mechanisms regulating melanocyte differentiation. In this study, we utilized primary cultures of neural crest cells and an immortalized melanocyte cell line (DMEL-2) which proliferates in the absence of TPA, to explore the effects of TPA on key melanogenic effectors. In neural crest cells, TPA was found to be necessary for both microphthalmia associated transcription factor (Mitf) up-regulation and for melanin synthesis. Using northern blots, we show that in DMEL-2 cells, TPA significantly increases the messenger ribonucleic acid
Phorbol ester-sensitive EL4 murine thymoma cells respond to phorbol 12-myristate 13-acetate with activation of ERK mitogen-activated protein kinases, synthesis of interleukin-2, and death, whereas phorbol ester-resistant variants of this cell line do not exhibit these responses. Additional aspects of the resistant phenotype were examined, using a newly-established resistant cell line. Phorbol ester induced morphological changes, ERK activation, calcium-dependent activation of the c-Jun N-terminal kinase (JNK), interleukin-2 synthesis, and growth inhibition in sensitive but not resistant cells. A series of protein kinase C activators caused membrane translocation of protein kinase Cs (PKCs) alpha, eta, and theta in both cell lines. While PKC eta was expressed at higher levels in sensitive than in resistant cells, overexpression of PKC eta did not restore phorbol ester-induced ERK activation to resistant cells. In sensitive cells, PKC activators had similar effects on cell viability and ERK ...
Phorbol ester tumor promoters and the anti-tumor-promoter dexamethasone share a molecular target: modulation of the transcription factor AP-1 by novel type of ...
DDT1 MF-2 cells, which are derived from hamster vas deferens smooth muscle, contain alpha 1-adrenergic receptors (54,800 +/- 2700 sites per cell) that are coupled to stimulation of inositol phospholipid metabolism. Incubation of these cells with tumor-promoting phorbol esters, which stimulate calcium- and phospholipid-dependent protein kinase, leads to a marked attenuation of the ability of alpha 1-receptor agonists such as norepinephrine to stimulate the turnover of inositol phospholipids. This turnover was measured by determining the 32P content of phosphatidylinositol and phosphatidic acid after prelabeling of the cellular ATP pool with 32Pi. These phorbol ester-treated cells also displayed a decrease in binding affinity of cellular alpha 1 receptors for agonists with no change in antagonist affinity. By using affinity chromatography on the affinity resin Affi-Gel-A55414, the alpha 1 receptors were purified approximately equal to 300-fold from control and phorbol ester-treated 32Pi-prelabeled ...
TY - JOUR. T1 - Anti-inflammatory effects of licorice and roasted licorice extracts on TPA-induced acute inflammation and collagen-induced arthritis in mice. AU - Chung, Won Yoon. AU - Kim, Ki Rim. AU - Jeong, Chan Kwon. AU - Park, Kwang Kyun. AU - Choi, Jong Hoon. AU - Park, Jung Han Yoon. AU - Lim, Soon Sung. PY - 2010/5/6. Y1 - 2010/5/6. N2 - The anti-inflammatory activity of licorice (LE) and roated licorice (rLE) extracts determined in the murine phorbol ester-induced acute inflammation model and collagen-induced arthritis (CIA) model of human rheumatoid arthritis. rLE possessed greater activity than LE in inhibiting phorbol ester-induced ear edema. Oral administration of LE or rLE reduced clinical arthritis score, paw swelling, and histopathological changes in a murine CIA. LE and rLE decreased the levels of proinflammatory cytokines in serum and matrix metalloproteinase-3 expression in the joints. Cell proliferation and cytokine secretion in response to type II collagen or ...
The activity of calcium-, phospholipid-dependent protein kinase (PKc) was measured in (a) total extracts, (b) crude membrane, and (c) cytosolic fractions of chick embryo myogenic cells differentiating in culture. Total PKc activity slowly declines during the course of terminal myogenesis in contrast to the activity of cAMP-dependent protein kinase, which was also measured in the same cells. Myogenic cells at day 1 of culture possess high particulate and low soluble PKc activity. A dramatic decline of particulate PKc activity occurs during myogenic cell differentiation and is accompanied, through day 4, by a striking rise of the soluble activity. The difference in the subcellular distribution of PKc between replicating myoblasts and myotubes is confirmed by phosphorylation studies conducted in intact cells. These studies demonstrate that four polypeptides whose phosphorylation is stimulated by the tumor promoter 12-O-tetradecanoyl phorbol 13-acetate in myotubes, are spontaneously phosphorylated ...
AP-1 transcriptional activity is stimulated by the transformation promoters phorbol 12-myristate 13-acetate ("12-O-tetradecanoylphorbol 13-acetate," TPA) and epidermal growth factor (EGF) in promotion-sensitive (P+) but not in promotion-resistant (P-) JB6 mouse epidermal cell lines. Although TPA stimulates expression of the jun and fos family genes, only c-jun expression shows higher elevation in P+ cells than in P- cells. The present study tests the hypothesis that induced AP-1 activity is required for tumor promoter-induced transformation in JB6 P+ cells. Both retinoic acid and the glucocorticoid fluocinolone acetonide inhibited basal and TPA-induced AP-1 activities that were tested with a stromelysin promoter-chloramphenicol acetyltransferase reporter gene in P+ cells. Since both retinoic acid and fluocinolone acetonide are active in inhibiting TPA-induced anchorage-independent transformation of P+ cells in the dose range that blocks TPA-induced AP-1 activity, their antipromoting effects may ...
TY - JOUR. T1 - Differential effects of protein kinase C on the levels of epithelial Na+ channel subunit proteins. AU - Stockand, James D. AU - Hui-Fang, B.. AU - Schenck, J.. AU - Malik, B.. AU - Middleton, P.. AU - Schlanger, L. E.. AU - Eaton, D. C.. PY - 2000/8/18. Y1 - 2000/8/18. N2 - Regulation of epithelial Na+ channel (ENaC) subunit levels by protein kinase C (PKC) was investigated in A6 cells. PKC activation altered ENaC subunit levels, differentially decreasing the levels of both β and γ, but not αENaC. Temporal regulation of β and γENaC by PKC differed; γENaC decreased with a time constant of3.7 ± 1.0 h, whereas βENaC decreased in 13.9 ±3.0h. Activation of PKC also resulted in a decrease in trans-epithelial Na+ reabsorption for up to 48h. PMA activation of PKC resulted in negative feedback inhibition of PKC protein levels beginning within 4h. Both β and γENaC levels, as well as transport tended toward pretreatment values after 48 h of PMA treatment. PKC inhibitors ...
TY - JOUR. T1 - Regulation of fibronectin gene expression by cyclic AMP and phorbol myristate acetate in HT-1080 human fibrosarcoma cells. AU - Lee, Byung Heon. AU - Park, Rang Woon. AU - Kim, In San. PY - 1998/12/31. Y1 - 1998/12/31. N2 - We studied the regulation of fibronectin (FN) gene expression by cAMP and phorbol-12-myristate-13-acetate (PMA) in HT-1080 human fibrosarcoma cells. Dibutyryl cAMP increased FN synthesis and mRNA levels, while PMA inhibited the cAMP-induced FN synthesis. In transient transfection assays, cAMP increased FN promoter activity, while PMA paradoxically enhanced the cAMP-induced promoter activity. Stable transfection experiments, however, showed that neither cAMP or PMA alone nor together affected FN promoter activity. These results suggest that PMA antagonizes the cAMP-induced FN gene expression and that both the action of cAMP and the inhibition of its action by PMA may occur at the posttranscriptional level in HT-1080 cells.. AB - We studied the regulation of ...
FIG. 3. Expression of PKC-α, -βI, and -βII isoforms in membrane and cytosolic fractions of DRG in experimental animals. Western blot analysis showed a single band of each isoform in all groups (A). Compared with nondiabetic littermate control mice (Lm) and transgenic mice (Tg), densitometric analysis disclosed reduced expression of membrane α isoform in both diabetic littermate mice (LmDM) and diabetic transgenic mice (TgDM), and the change in diabetic transgenic mice was more severe than in diabetic littermate mice (B). By contrast, cytosolic fraction of α isoform was contrariwise increased to a similar extent in both diabetic transgenic mice and diabetic littermate mice. Treatment with an ARI (fidarestat) corrected these changes in both diabetic groups (LmDM+ARI and TgDM+ARI). There was no change in βI expression in either membrane or cytosolic fraction among all groups. On the other hand, membrane βII expression tended to be elevated in diabetic littermate mice, and the increase was ...
B lymphocytes are necessary cells in defense replies. B lymphocytes HVCN1S appearance is normally higher in B-cell lines and in B cells from sufferers with chronic lymphocytic leukemia where it could donate to disease pathogenesis. and and relationship did not differ significantly. HVCN1S Responds More Strongly Avasimibe Avasimibe to PKC-Dependent Phosphorylation. Proton currents in phagocytes and other cells are greatly augmented by phosphorylation of the channel by PKC (8). The enhanced gating response is usually stimulated effectively by the PKC activator PMA (phorbol myristate acetate) and is best analyzed using the perforated-patch configuration that preserves intracellular signaling pathways (9). Fig. 2 illustrates families of proton currents in cells expressing HVCN1L and HVCN1S before and after PMA activation. In response to PMA the currents turn on more rapidly and at more unfavorable voltages and turn off more slowly and the current amplitude is usually increased. Although HVCN1L ...
Quercetin (QUE; 3,5,7,3′,4′-tetrahydroxyflavone) has been shown to possess several beneficial biological activities including antitumor, anti-inflammation and antioxidant properties; however, the effects of QUE in preventing invasion by breast carcinoma cells are still undefined. Increases in the protein, messenger RNA and enzyme activity levels of matrix metalloproteinase (MMP)-9 were observed in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 cells, and these were blocked by QUE, but not by quercitrin or rutin. A translocation of protein kinase C (PKC)δ from the cytosol to the membrane followed by activation of extracellular signal-regulated kinase (ERK) and c-Jun/activator protein-1 (AP-1) by TPA was demonstrated, and TPA-induced MMP-9 activation and migration were inhibited by the pan PKC inhibitor, GF109203X, the specific PKCδ inhibitor, rottlerin, an ERK inhibitor (PD98059) and an AP-1 inhibitor (curcumin). Application of QUE significantly suppressed TPA-induced activation ...
Author: Rauscher, Andreas et al.; Genre: Journal Article; Published in Print: 2000-09-07; Keywords: Vimentin; Gene expression; Electroporation; Stress; 12-O-Tetradecanoylphorbol-13-acetate; Plasmacytoma; MPC-11 cell; Title: Similar effects of electroporational stress and treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate on vimentin expression in mouse plasmacytoma cells
Spirulina, a water blue-green microalga, is considered a complex natural product that is widely used in treatment of chronic diseases including cancer, hypercholesterolemia, arterial hypertension, obesity and diabetes. Phycocyanin from spirulina is considered to be a strong radical scavenger (hydroxyl, peroxyl and alkoxyl radicals) providing significant antioxidant and anti-inflammatory effects. The aim of this study consists in the evaluation of the anti-inflammatory effect of SP in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear inflammation in hairless SKH1 mice. The ears of mice treated with a higher concentration of SP (1000 μg/mL) showed a significant reduction of the inflammatory process than those treated with a smaller concentration of SP (200 μg/mL). Consequently, spirulina has proved dose-dependent anti-inflammatory effects in controlling and, also, in improving the acute inflammation process in mice, being a future alternative therapy for treating inflammation diseases ...
Spirulina, a water blue-green microalga, is considered a complex natural product that is widely used in treatment of chronic diseases including cancer, hypercholesterolemia, arterial hypertension, obesity and diabetes. Phycocyanin from spirulina is considered to be a strong radical scavenger (hydroxyl, peroxyl and alkoxyl radicals) providing significant antioxidant and anti-inflammatory effects. The aim of this study consists in the evaluation of the anti-inflammatory effect of SP in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear inflammation in hairless SKH1 mice. The ears of mice treated with a higher concentration of SP (1000 μg/mL) showed a significant reduction of the inflammatory process than those treated with a smaller concentration of SP (200 μg/mL). Consequently, spirulina has proved dose-dependent anti-inflammatory effects in controlling and, also, in improving the acute inflammation process in mice, being a future alternative therapy for treating inflammation diseases ...
The expression of proteases such as MMP-9 is regulated by diverse growth factors, cytokines, and xenobiotics such as PMA. Studies have shown that the mechanism responsible for PMA-mediated responses may involve direct alteration of transcription factors, but these mechanisms are not completely understood. Small molecular weight inhibitors that target the pathways that regulate MMP-9 expression could improve our understanding of these pathways and potentially be of clinical utility for the treatment of cancer. Using a cervical cancer cell line, our study demonstrates the ability of dykellic acid to reduce the expression of MMP-9.. We also investigated the molecular mechanism by which dykellic acid inhibits PMA-mediated expression of MMP-9 using AP-1 and NFκB reporter constructs and found that NFκB activity, but not AP-1 activity, is significantly reduced by treatment with dykellic acid. Thus, dykellic acid suppresses expression of MMP-9 via inhibition of NFκB transactivation. To our knowledge, ...