Developmentally, the pancreas and liver are closely related and pathological conditions − including elevated glucocorticoid levels − result in the appearance of hepatocytes in the pancreas. The role of the WNT signalling pathway in this process has been examined in the model transdifferentiating pancreatic acinar AR42J-B-13 (B-13) cell. Glucocorticoid treatment resulted in a transient loss of constitutive WNT3a expression, phosphorylation and depletion of β-catenin, loss of β-catenin nuclear localisation, and significant reductions in T-cell factor/lymphoid enhancer factor (Tcf/Lef) transcriptional activity before overt changes in phenotype into hepatocyte-like (B-13/H) cells. A return to higher Tcf/Lef transcriptional activity correlated with the re-expression of WNT3a in B-13/H cells. β-catenin knock down alone substituted for and enhanced glucocorticoid-dependent transdifferentiation. Overexpression of a mutant β-catenin (pt-Xβ-cat) protein that blocked glucocorticoid-dependent ...
Akt-regulated pathways enhance cell division and cell survival. Metabolic regulation through Akt and its targets is important for insulin and insulin-like growth factor I-coupled responses. Inhibition of glycogen synthase kinase-3 by Akt-dependent phosphorylation promotes accumulation of β-catenin, which forms complexes with T-cell factor/lymphoid enhancer factor transcription factors and transcriptionally up-regulates cyclin D1, Myc, and other positive growth regulators. Cyclin D1 is also inhibited by glycogen synthase kinase-3 through effects on stability and localization (7) . Concomitantly, Akt phosphorylation of cyclin-dependent protein kinase inhibitors p21Cip1 and p27Kip1 interferes with negative growth regulation (3 , 8) .. Phosphorylation of Mdm2 by Akt enhances nuclear entry, which promotes ubiquitin-dependent proteolysis of p53, and impedes p53-dependent growth suppression and apoptosis (9) . Akt directly forestalls apoptosis by phosphorylation of proapoptotic Bad and caspase-9, ...
Complete information for TCF15 gene (Protein Coding), Transcription Factor 15, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Recent theoretical work by Reichl and Mueller (to be published in Physical Review A) describes how to engineer and probe an exotic state of matter called a "topological insulator" in a gas of atoms cooled to temperatures near absolute zero. Topological insulators have the remarkable property that they act like insulators in their interior but can conduct along their surfaces or edges. They are not only of fundamental interest to physicists but have important applications in electronics and quantum computation. To engineer such a state, the authors imagine an experiment where ultracold atoms are trapped in a lattice with laser beams and made to behave like a topological insulator by modulating the lasers to change the shape of the lattice periodically in time. The smoking-gun signature of a topological insulator is the presence of "edge states"- conduction channels appearing at the boundaries of the system. Using computer simulations, the authors demonstrate how a clump of atoms released at the ...
Glycogen synthase kinase 3 (GSK3) was discovered as a metabolic enzyme, that regulates the response of glycogen synthase to insulin; however, it is now known to be active in a wide range of cellular processes, ranging from apoptosis to embryonic development (Cohen and Frame, 2001; Frame and Cohen, 2001; Harwood, 2001). In animal development, it is best known for its activity in Wnt signalling, where it regulates cellular differentiation, migration and growth (Miller, 2002). In the canonical Wnt/β-catenin pathway, cell stimulation by Wnt protein ligands leads to the accumulation and nuclear translocation of β-catenin, which acts as a transactivator for genes regulated by T-cell factor/lymphoid enhancer factor (TCF/LEF) (reviewed by Brantjes et al., 2002).. Dictyostelium has a single GSK3 homologue, GskA, that has 78% amino acid identity to mammalian GSK3β (Harwood et al., 1995). Upon starvation, neighbouring Dictyostelium amoebae aggregate, using cAMP as chemoattractant. The cells then ...
SMAD4 has been suggested to inhibit the activity of WNT/beta-catenin signaling pathway in cancer. However, the mechanism by which SMAD4 antagonizes WNT/beta-catenin signaling in cancer remains largely unknown. Aurora A kinase (AURKA), which is frequently overexpressed in cancer, increases the transcriptional activity of beta-catenin/T cell factor (TCF) complex by stabilizing beta-catenin through the inhibition of GSK-3beta. Here, SMAD4 modulated AURKA in a TGF-beta-independent manner. Overexpression of SMAD4 significantly suppressed AURKA function including colony formation, migration, and invasion of cell lines. In addition, SMAD4 bound to AURKA, induced degradation of AURKA by the proteasome. A luciferase activity assay revealed that the transcriptional activity of the beta-catenin/TCF complex was elevated by AURKA, but decreased by SMAD4 overexpression. Moreover, target gene analysis showed that SMAD4 abrogated the AURKA-mediated increase of beta-catenin target genes. However, this inhibitory ...
Previous studies have shown that E-protein transcription factors in the thymus have important roles, both in the very earliest stages of thymocyte development and in the regulation of key thymocyte transitions (Murre 2005; Kee 2009; and Jones and Zhuang, 2011). In particular, they have provided convincing evidence that preTCR and TCR signaling leads to successively more profound down-regulation of E-protein levels and that such down-regulation is a precondition for DN to DP transitions and DP to SP transitions (Engel et al., 2001; Jones and Zhuang, 2007; Jones-Mason et al., 2012). Here, we used inducible E-protein KO mice (E2Af/f/HEBf/f/Er-cre mice) to study the effect of E-proteins (and the related effect of Id proteins) on the development of Foxp3+ T reg cells. We found that induced Foxp3+ T reg cell (iT reg cell) development in peripheral tissues was significantly increased both in vitro and in vivo in E-protein KO mice. In addition, this increase in iT reg cell development was accompanied by ...
Wnt signaling controls diverse developmental processes such as axis formation, anteroposterior patterning and the development of the neural crest (Moon et al., 1997; Raible and Ragland, 2005; Wodarz and Nusse, 1998). Precise regulation is necessary for many developmental processes and misregulation of several components of the canonical Wnt signaling pathway has been implicated in cancer formation (Polakis, 2007). Conversely, reduction or loss of function of Wnt signaling leads to general developmental defects or loss of organs, demonstrating the need for a tight regulation of the levels of Wnt signaling (Logan and Nusse, 2004).. The Wnt signaling pathway is highly conserved among all metazoans. Wnt ligands bind to frizzled (fz) transmembrane receptors leading to accumulation and nuclear localization of β-catenin, which serves as a transcriptional co-activator for TCF/Lef transcription factors (Logan and Nusse, 2004; Wodarz and Nusse, 1998). In addition to this so called `canonical Wnt ...
By means of the fluorescent differential display method, we isolated novel mouse and human genes, Drctnnb1a and DRCTNNB1A, the expression levels of which were inversely correlated to the amount of β-catenin present in cells. Recent reports have identified a number of mammalian genes including c-myc (6) , cyclin D1 (7) , matrilysin (8) , WISP (9) , c-jun, fra-1, uPAR, ZO-1 (10) , and NBL4 (11) that are regulated by stabilization and activation of β-catenin. In Xenopus or Drosophila, target genes for Wnt signaling include the nodal-related 3 gene, Xnr3 (17) , a member of the transforming growth factor-β superfamily, and homeobox genes engrailed (18) , goosecoid, siamois (17) , twin (19) , ultrabithorax (20) , and fibronectin (21) . Among those reported molecules, all but ZO-1 appeared to be up-regulated byβ -catenin through transactivation of Tcf/Lef transcription factors. Hence, DRCTNNB1A is only the second gene to be identified as down-regulated by the accumulation of β-catenin. ...
Sigma-Aldrich offers abstracts and full-text articles by [Laurent Pangon, Dessislava Mladenova, Lauren Watkins, Christa Van Kralingen, Nicola Currey, Sam Al-Sohaily, Patrick Lecine, Jean-Paul Borg, Maija R J Kohonen-Corish].
Evidence to date indicates the predominance of activation of β-catenin/TCF-regulated target genes following Wnt pathway deregulation in the development and progression of cancer. The present study offers evidence that PLD1 drives a positive feedback loop to reinforce the Wnt/β-catenin/TCF signaling axis. We found that injection of LiCl into mice significantly increased the level of PLD1 protein in colon tissues (data not shown), suggesting its physiologic relevance in vivo. β-Catenin and TCF-4 elevated the expression and activity of PLD1. We were able to define three functional TCF binding sites within the PLD1 promoter, suggesting that the PLD1 gene is a direct transcriptional target of β-catenin/TCF signaling. We found that the mRNA level and promoter activity of PLD1 were diminished by wild-type ectopic expression of APC, axin, and GSK3β, whereas expression of Dvl3 and inactive mutant APC as positive regulators of Wnt signaling increased expression of PLD1 (data not shown). Thus, it is ...
Tcf4 associates with the promoter of miR-181s. (A) Predicted Tcf4 binding sites in the promoter region of the miR-181a-2/miR-181b-2 gene. (B) PCR amplicons for
Homo sapiens transcription factor 7 (T-cell specific, HMG-box) (TCF7), transcript variant 1, mRNA. (H00006932-R01) - Products - Abnova
Canonical Wnt signaling is mediated by a molecular "switch" that regulates the transcriptional properties of the T-cell factor (TCF) family of DNA-binding proteins. Members of the myeloid translocation gene (MTG) family of transcriptional corepressors are frequently disrupted by chromosomal translocations in acute myeloid leukemia, whereas MTG16 may be inactivated in up to 40% of breast cancer and MTG8 is a candidate cancer gene in colorectal carcinoma. Genetic studies imply that this corepressor family may function in stem cells. Given that mice lacking Myeloid Translocation Gene Related-1 (Mtgr1) fail to maintain the secretory lineage in the small intestine, we surveyed transcription factors that might recruit Mtgr1 in intestinal stem cells or progenitor cells and found that MTG family members associate specifically with TCF4. Coexpression of beta-catenin disrupted the association between these corepressors and TCF4. Furthermore, when expressed in Xenopus embryos, MTG family members inhibited ...
In the canonical Wnt signaling pathway, beta-catenin activates target genes through its interactions with Tcf/Lef-family transcription factors and additional transcriptional coactivators. The crystal structure of ICAT, an inhibitor of beta-catenin-mediated transcription, bound to the armadillo repeat domain of beta-catenin, has been determined. ICAT contains an N-terminal helilical domain that binds to repeats 11 and 12 of beta-catenin, and an extended C-terminal region that binds to repeats 5-10 in a manner similar to that of Tcfs and other beta-catenin ligands. Full-length ICAT dissociates complexes of beta-catenin, Lef-1, and the transcriptional coactivator p300, whereas the helical domain alone selectively blocks binding to p300. The C-terminal armadillo repeats of beta-catenin may be an attractive target for compounds designed to disrupt aberrant beta-catenin-mediated transcription associated with various cancers. ICAT inhibits beta-catenin binding to Tcf/Lef-family transcription factors ...
CG-001 is a selective Wnt/β-catenin signalling inhibitor with an IC50 of 3μM. ICG 001, a small molecule that down-regulates beta-catenin/T cell factor signaling by specifically binding to cyclic AMP response element-binding protein. ICG001 selectively ind
T-cell factor 3 (TCF3), a downstream effector of Wnt signaling in embryonic stem (ES) cells, plays an important role in pluripotent self-renewal and proliferation. Loss of TCF3 delays the differentiation of mouse ES cells. The purpose of this study was to investigate the effect of TCF3 on embryonal carcinoma (EC). The mouse F9 EC cell line and a tumor-bearing mouse model were used to evaluate the anti-EC tumor effects of TCF3 in vitro and in vivo, respectively. The overexpression of TCF3 significantly inhibited proliferation, colony-forming and migration in F9 EC cells by approximately 30, 45 and 30%, respectively. The in vivo mouse model showed that the overexpression of TCF3 significantly reduced tumor volume (36.4%) and tumor weight (34.8%), malignancy progression and local infiltration and prolonged the life span of tumor-bearing mice. Overexpression of TCF3 significantly down-regulated Oct4 expression in the F9 EC cells. The results indicate that TCF3 is an inhibitor of the malignant ...
TGF-β is a key profibrotic factor, but targeting TGF-β to prevent fibrosis also abolishes its protective anti-inflammatory effects. Here, we investigated the hypothesis that we can redirect TGF-β signaling by preventing downstream profibrotic interaction of β-catenin with T cell factor (TCF), thereby enhancing the interaction of β-catenin with Foxo, a transcription factor that controls differentiation of TGF-β induced regulatory T cells (iTregs), and thus, enhance anti-inflammatory effects of TGF-β In iTregs derived from EL4 T cells treated with recombinant human TGF-β1 (rhTGF-β1) in vitro, inhibition of β-catenin/TCF transcription with ICG-001 increased Foxp3 expression, interaction of β-catenin and Foxo1, binding of Foxo1 to the Foxp3 promoter, and Foxo transcriptional activity ...
Our current work focuses on neurogenesis in the spinal cord and the hypothalamus, addressing three important questions. 1) Which cells require Wnt/Tcf signaling? 2) What are the molecular targets of Tcf proteins? 3) How does Wnt/Tcf-dependent neurogenesis contribute to physiology and behavior?. ...
TCF3 - TCF3 (untagged)-Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 2 available for purchase from OriGene - Your Gene Company.
TCF4 - TCF4 (Myc-DDK-tagged)-Human transcription factor 4 (TCF4), transcript variant 1 available for purchase from OriGene - Your Gene Company.
There is confusion about the initiative. This has arisen through the twin evils of complacency and assumption. Some firms thought they had satisfied customers and received a low level of complaints so they must be treating their customers fairly. Others have always been confused about how the principles of TCF apply to them on an everyday basis.. Cynics would say TCF isnt the most rivetting of subjects but my response is that it is too important to ignore. If you are not factoring TCF into your activities you had better start right now. That sounds like a lecture but wherever you are in the market you have to understand the importance with which the FSA views TCF.. The regulators approach to TCF is interesting. Its initial rules-based approach has been changed by the introduction of TCF because the initiative is principles rather than rules-based. This approach by the FSA is to be commended. Instead of being swamped by rules we have guidelines.. With TCF, the starting point is the principle ...
Expression of TCF3 (bHLHb21, E2A, E47, ITF1, MGC129647, MGC129648, VDIR) in duodenum tissue. Antibody staining with HPA049808, HPA062476 and CAB018351 in immunohistochemistry.
We have shown that LEF1 played critical and nonredundant roles in iNKT cell expansion and iNKT2 effector fate differentiation. We showed that in the absence of LEF1, iNKT cell expansion at ST0 and ST1 failed to occur and that LEF1 directly regulated expression of the CD127 component of the IL-7 receptor and the transcription factor c-myc, both of which are required for this phase of expansion (Dose et al., 2009; Mycko et al., 2009; Tani-ichi et al., 2013). LEF1 was also required for the development of iNKT2 cells, which include both IL-4 only and IL-4 plus IFNγ-producing iNKT cells, and LEF1 directly regulated the expression of the iNKT2 signature transcription factor GATA3. Our findings are particularly striking given that LEF1 deficiency has only subtle effects on conventional T cells during their differentiation and in the periphery during their activation and acquisition of the memory phenotype as the result of redundancy with the related transcription factor TCF1 (Okamura et al., 1998; Yu ...
Journal of Neurosurgery, Volume 0, Issue 0, Page 1-9, Ahead of Print.. Fengming Lan, M.S., Xiao Yue, M.S., Lei Han, Ph.D., Xubo Yuan, Ph.D., Zhendong Shi, M.S., Kai Huang, M.S., Yang Yang, M.S., Jian Zou, M.S., Junxia Zhang, Ph.D., Tao Jiang, M.D., Ph.D., Peiyu Pu, M.D., Ph.D., and Chunsheng Kang, M.D., Ph.D.. Object. The goal in this study was to investigate the antitumor effect of aspirin in glioblastoma cells and the molecular mechanism involved in its antineoplastic activities.. Methods. The authors used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method, flow cytometry, the annexin V method, and Transwell cell invasion test to detect the proliferation and invasive activity of U87 and A172 glioma cells before and after being treated with aspirin. To determine the effects of aspirin on β-catenin/T-cell factor (TCF) transcription activity, reporter constructs containing 3 repeats of the wild-type (TOPflash) or mutant (FOPflash) TCF-binding sites were used. Reverse ...
Aberrant expression of the genes involved in Wnt signaling pathway, one of the most important developing pathways, is observed in many malignancies. Reports show that Wnt/β-catenin activation is critical for cancer development, angiogenesis, migration, and invasion. LEF1 belongs to the T cell Factor (TCF)/LEF family of transcription factors and plays the role of nuclear effector in the Wnt/β-catenin signaling pathway. LEF1 has central role as a transcription factor in the Wnt/β-catenin signaling pathway which makes it an ideal target for therapeutic treatment in dealing with cancer proliferation. It can act as an oncogene or a tumor suppressor in cellular context dependent manner. miRNAs are aberrantly expressed in cancers and can act as tumor suppressors or oncomirs depending upon the type of carcinomas. Studies show that miRNAs can be used as novel agents for targeted cancer therapy. miR-106b, which belong to miR-17-92 paralog cluster, is reported to be overexpressed in multiple tumor types
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
negative regulator of transcriptional activity repressing expression of SPP1 and beta-catenin/TCF7L2 target genes, which are involved in myogenesis, muscle maintenance, and regeneration in a histone deacetylase dependent ...
rat Tcf8 protein: a human homolog of chicken delta EF1 protein; binds T3-response elements; has 2 isoforms Zfhep-1 & Zfhep-2; amino acid sequence given in first source
DEBT AND EQUITIES TCF Financial Corp., Wayzata, said it raised gross proceeds of $100 million through its previously announced public offering of 6.45 percent Series B non-cumulative perpetual preferred stock. In connection with the offering, TCF issued 4 million shares at a public offering price of $25 per share. The underwriters have an option to purchase up to an additional 600,000 preferred shares to cover overallotments at the same price, for potential additional gross proceeds of $15 million. Net proceeds of the offering to TCF were about $96.5 million, which TCF expects to use for general corporate purposes.. Mocon Inc., Brooklyn Park, declared a quarterly cash dividend of 10.5 cents a share, payable Feb. 15 to shareholders of record on Feb. 1.. ...
Hopus is a Belgian IPA style beer brewed by Brasserie Lefèbvre SA in Rebecq-Quenast, Belgium. 3.73 average with 352 ratings, reviews and opinions.
Aberrant activation of Wnt/beta-catenin signaling is recognized as a critical factor in the etiology of colorectal cancer. Evidence has suggested that dysregulated beta-catenin activity is associated with the majority of colon cancers via activation of the expression of Wnt regulated oncogenes. In the nucleus, beta-catenin regulates transcription by recruiting additional coactivators. These coactivators all have distinct and unique functions on Wnt/beta-catenin target gene activation. Here we report two coactivators for beta-catenin-mediated transcription: CCAR1 (Cell Cycle and Apoptosis Regulator 1) and CARM1 (coactivator-associated-protein-arginine-methyltransferase 1). We show that both CCAR1 and CARM1 interact with beta-catenin and positively modulate beta-catenin-mediated gene expression. In colorectal cancer cells, which have constitutively high Wnt/beta-catenin activity, depletion of CCAR1 or CARM1 inhibits the expression of Wnt/beta-catenin-mediated oncogenes and suppresses ...
The role of the TCF family of transcriptional regulators in primary axis formation is addressed by studying the mechanisms of action of XTcf-3 in Xenophus laevis embryos. The early events of primary axis induction involve activation through the WNT signaling pathway. As a result of activation ... read more of the pathway the cytoplasmic level of ß-catenin increases at the future dorsal side of the early cleavage stage embryo. Around the 16-32 cell stage, ß-catenin becomes apparent in the nuclei. The presence of nuclear ß-catenin causes several hours later the activation of specific target genes, like e.g. XSiamois. Since ß-catenin does not contain a DNA binding region, DNA binding proteins must mediate this transactivation. Ectopic expression of ß-catenin causes activation of dorsal genes and results in the induction and differentiation of a secondary axis. (See introduction). Three different homologs of the Tcf/Lef family of transcription factors have been cloned in Xenopus laevis, XTcf-3, ...
Burkitt lymphoma (BL) is an aggressive B-cell lymphoma characterized by translocation and deregulation of the proto-oncogene c-MYC.Transcription factor 3(TCF3) has also been shown to be involved in BL pathogenesis. In BL, TCF3 is constitutively active,and/orexpression of its transcriptional targetsare alteredas a result of BL-associated mutations. Here, we found that BL-related TCF3mutations affect TCF3 alternative splicing,in part by reducing binding of the splicing regulatorhnRNPH1 to exon 18b. This leads to greater exon 18b inclusion, thereby generating more of the mutated E47 isoform of TCF3. Interestingly, upregulationof E47 dysregulates expression of TCF3 targets PTPN6, and perhaps CCND3, whichareknown to be involved in BL pathogenesis. Our findings thus reveal a mechanismby whichTCF3 somatic mutations affectmultilayered gene regulation underlying BL pathogenesis. ...
A novel role of TCF family in body axis formation. Revolutionary high impact discoveries are described, elucidating the missing link in the Wnt pathway and protein-TCF combinations with dual functions. By studying the primary axis formation of Xenopus laevis, it was firstly shown that, in combination with beta-catenin, TCF acts as a potent activator of proto-oncogenes. Secondly, it was discovered that in combination with the Groucho family of proteins, TCF acts as suppressor of oncogene transcription. Stronlgy suggesting that TCF controls oncogene transcription in a dual fashion. These discoveries contributed to the origination of a major area of cancer research and opened multiple angles for cancer therapy development ...
TCF4 Antibody is a Rabbit Polyclonal antibody against TCF4. This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box (E-box) binding site (CANNTG) - a motif first identifie
Common intronic variation within the gene encoding transcription factor 7-like 2 (TCF7L2) is now considered to be definitively associated with type 2 diabetes (T2D). Since our first report of this association in 2006 (1), independent investigators have readily replicated this finding in all the main ethnic groups; in addition, from the first genome-wide association study (GWAS) of T2D in Caucasians (2) onwards, the strongest association has been consistently with the TCF7L2 locus. Indeed, a meta-analysis of published studies worldwide as early as 2007 estimated a pooled odds ratio of 1.46 (with an impressive P = 5.4 × 10−140) (3), making it one of the most statistically significant genetic findings in T2D to date.. Despite knowing that this association has been beyond doubt for over 6 years, the mechanism through which TCF7L2 exerts its effect on T2D is still very unclear, thus making the work by Kaminska et al. (4) in this current issue of Diabetes so timely.. TCF7L2 is a high-mobility group ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Mouse monoclonal antibody raised against a partial recombinant TCF7L2. TCF7L2 (NP_110383, 490 a.a. ~ 596 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. (H00006934-M05) - Products - Abnova
Sigma-Aldrich offers abstracts and full-text articles by [E Sánchez-Tilló, O de Barrios, E Valls, D S Darling, A Castells, A Postigo].
NOTICE : As of 5-25-2010 TCF-1 was increased from a 4oz bottle to an 8oz bottle, doubling the number of servings from 12 to 24. This is twice as many
Kit Component:- KN306359G1, Gcfc2 gRNA vector 1 in pCas-Guide vector- KN306359G2, Gcfc2 gRNA vector 2 in pCas-Guide vector- KN306359D, donor vector…
DasGupta et al. [3] developed a high-throughput assay based on the known ability of canonical Wnt signaling to activate transcription of luciferase reporter constructs in transfected cells. Improving on the widely used construct TOP-Flash [13], they generated two new reporters each containing multiple TCF-binding sites upstream of a different minimal promoter. Because only the TCF sites were common between the reporters, off-target effects unrelated to β-catenin/TCF signaling were minimized. Reporters with mutated TCF-binding sites also served as specificity controls. The authors first validated the behavior of these reporters in transfection assays of Drosophila cell lines. Then they scaled up the transfections to incorporate approximately 22,000 double-stranded RNAs (dsRNAs), so as to induce RNAi [3], and tested the individual effects on Wingless-induced signaling. The library of dsRNA sequences, previously used in other high-throughput RNAi screens, is directed at all known open reading ...
Profile: Publications and Interests. https://jhu.pure.elsevier.com/en/persons/jyoti-misra-sen. Selected Publications. Yu, Q., Sharma, A., Oh, S. Y., Moon, H. G., Hossain, M. Z., Salay, T. M., Leeds, K. E., Du, H., Wu, B., Waterman, M. L., Zhu, Z., Sen. J. M. T cell factor 1 initiates the T helper type 2 fate by inducing the transcription factor GATA-3 and repressing interferon-gamma. Nature Immunology. 9: 992-9, 2009.. Yu Q, Sharma A, Sen J. M. TCF1 and beta-catenin regulate T cell development and function. Immunological Research. 47: 45-55, 2010.. Yu, Q., Sharma, A., Ghosh, A. and Sen, J. M. T Cell Factor-1 negatively regulates expression of IL-17 family of cytokines and protects mice from experimental autoimmune encephalomyelitis. Journal of Immunology. 186:3946-52. 2011. Comment. JI 186:3801.. Sharma, A., Chen, Q., Nguyen, T., Yu, Q. and Sen, J. M. T cell factor-1 and ?eta-catenin control the development of memory-like CD8 thymocytes. 2011. Journal of Immunology. 188:3859-68, 2012 issue. ...
Using a combination of gain- and loss-of-function experiments, we showed that CD36 is downstream of Meox2/Tcf15-mediated uptake of FFAs originating from both bovine serum albumin conjugates and VLDL particles in heart ECs. The high CD36 expression levels in comparison with other transporter genes in heart ECs further support its important role in cardiac FA uptake. Nevertheless, although, in our DiI-VLDL assay, CD36s contribution to Meox2/Tcf15-driven FFA uptake could have been underestimated by simultaneous detection of particle uptake, the fact that CD36 knockdown only partially impaired Meox2/Tcf15-driven uptake of BODIPY-labeled FFAs suggests the existence of other Meox2/Tcf15 downstream mediators or the occurrence of compensatory mechanisms upon CD36 knockdown.. In Meox2+/−:Tcf15+/− heart ECs, Fatp3, a FA transporter regulated by the paracrine VEGF-B,21 was increased, but this was not sufficient to fully compensate for the partial loss of CD36 induced by Meox2+Tcf15 haplodeficiency, ...
Dysregulation of Wnt signaling is closely associated with human liver tumorigenesis. However, liver cancer-specific Wnt transcriptional programs and downstream effectors remain poorly understood. Here, we identify tribbles homolog 2 (TRIB2) as a direct target of Wnt/TCF in liver cancer and demonstra …
Tcf7l2 (untagged) - Mouse transcription factor 7-like 2, T-cell specific, HMG-box (Tcf7l2), transcript variant 7, (10ug), 10 µg.
TCF21 encodes a transcription factor of the basic helix-loop-helix family. The TCF21 product is mesoderm specific, and expressed in embryonic epicardium, mesenchyme-derived tissues of lung, gut, gonad, and both mesenchymal and glomerular epithelial cells in the kidney. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] ...
Beta-catenin/Tcf reporter mice. These mice express GFP gene under the control of the Tcf/Lef binding sites and a minimal thymidine kinase promoter from the TOPFLASH plasmid. Immunohistochemistry with a GFP antibody can be used to assess the Tg expression in the intestine. In other organs except for the intestine. In the splenocytes, expression of GFP was observed by FACS analysis, In other organs, the GFP expression patterns are not examined yet ...