A sensor is described which utilizes the phenomenon of surface plasmon resonance to detect changes in refractive index of chemical or biochemical samples applied to a surface modified optical fiber. The sensor is constructed by polishing a short section of the lateral surface of an optical fiber to its evanescent field surrounding the fiber core. One or more thin films are applied to the polished section of the fiber to produce the sensing element. One of the films is the metal silver, which acts as the support for the surface plasmon. Under the proper conditions, TM polarized energy propagating in the fiber can be coupled to a surface plasmon electromagnetic mode on the metal film. This coupling depends on the wavelength, the nature of the fiber, the refractive index and thickness of the thin films applied to the fiber, and the refractive index of a chemical sample in contact with the modified surface. The fiber to plasmon coupling is seen as a large attenuation of the light reaching the distal ...

TY - GEN. T1 - Evaluation of an affinity-amplified immunoassay of graphene oxide using surface plasmon resonance biosensors. AU - Chiu, Nan-Fu. AU - Huang, Teng Yi. AU - Kuo, Chun Chuan. PY - 2015/1/1. Y1 - 2015/1/1. N2 - We describe a fundamental study on the plasmonic properties and advanced biosensing mechanisms of functionalized graphene. We discuss a specific design using modified carboxyl groups, which can modulate surface plasmon (SP) coupling and provide an advantage for their binding to the sensing layer with high-performance affinity in an immunological reaction. The functionalized graphene-based surface plasmon resonance (SPR) biosensors have three advantages: high performance, high sensitivity, and excellent molecular kinetic response. In the future, functionalized graphene sheets will make a unique contribution to photonic and SPR diagnosis devices. We wish to highlight the essential characteristics of functionalized graphene-based SPR biosensors to assist researchers in developing ...

TY - JOUR. T1 - Ultra-high sensitivity of the non-immunological affinity of graphene oxide-peptide-based surface plasmon resonance biosensors to detect human chorionic gonadotropin. AU - Chiu, Nan Fu. AU - Kuo, Chia Tzu. AU - Lin, Ting Li. AU - Chang, Chia Chen. AU - Chen, Chen Yu. PY - 2017/8/15. Y1 - 2017/8/15. N2 - Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance as sensing probes due to their potential in the development of non-immunological assays with high sensitivity, affinity and specificity for human chorionic gonadotropin (hCG) protein. We combined graphene oxide (GO) sheets with a specific peptide aptamer to create a novel, simple and label-free tool to detect abnormalities at an early stage of pregnancy, a GO-peptide-based surface plasmon resonance (SPR) biosensor. This is the first binding interface experiment to successfully demonstrate binding specificity in kinetic analysis biomechanics in peptide aptamers ...

TY - JOUR. T1 - An improved immunoassay for detection of saxitoxin by surface plasmon resonance biosensors. AU - Yakes, B.J.. AU - Prezioso, S.. AU - Haughey, S.A.. AU - Campbell, Katrina. AU - Elliott, Christopher. AU - DeGrasse, S.L.. PY - 2011/8. Y1 - 2011/8. N2 - Saxitoxin and its analogs, the causative agents of paralytic shellfish poisoning (PSP), are a worldwide threat to seafood safety. Effective monitoring of potentially contaminated fishing areas as well as screening of seafood samples is necessary to adequately protect the public. While many analytical methods exist for detecting paralytic shellfish toxins (PSTs), each technique has challenges associated with routine use. One recently developed method [1] that overcomes ethical or performance-related issues of other techniques is the surface plasmon resonance (SPR) bioassay. Notwithstanding the advantages of this method, much research remains in optimizing the sensor substrate and assay conditions to create a robust technique for ...

TY - JOUR. T1 - Application of surface plasmon coupled emission to study of muscle. AU - Borejdo, J.. AU - Gryczynski, Z.. AU - Calander, N.. AU - Muthu, P.. AU - Gryczynski, I.. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2006/10. Y1 - 2006/10. N2 - Muscle contraction results from interactions between actin and myosin cross-bridges. Dynamics of this interaction may be quite different in contracting muscle than in vitro because of the molecular crowding. In addition, each cross-bridge of contracting muscle is in a different stage of its mechanochemical cycle, and so temporal measurements are time averages. To avoid complications related to crowding and averaging, it is necessary to follow time behavior of a single cross-bridge in muscle. To be able to do so, it is necessary to collect data from an extremely small volume (an attoliter, 10 -18 liter). We report here on a novel microscopic application of surface plasmon-coupled emission (SPCE), which provides such a ...

TY - JOUR. T1 - Surface plasmon resonance and emitted light properties of polystyrene sphere films. AU - Shinbo, Kazunari. AU - Miyabayashi, Syunsuke. AU - Yoshizawa, Kazushi. AU - Shimizu, Hidehiko. AU - Kato, Keizo. AU - Kaneko, Futao. AU - Tanaka, Masato. AU - Wakamatsu, Takashi. AU - Advincula, Rigoberto C.. PY - 2003. Y1 - 2003. N2 - Polystyrene sphere films were fabricated and the optical properties were investigated using surface plasmon spectroscopy (SPS) and emitted light due to reverse irradiation utilizing SPS Kretschmann configuration. The films were fabricated using sphere dispersed solution with various diameters from about 100 to 300 nm. The morphologies of the fabricated films were observed using atomic force microscopy and the sphere films had almost three layer structure. In SPS curve, large dip and shallow broad dip were observed at around 75° and 50°, respectively. The dips were considered to be due to the three layer structure and some defects in the film. Furthermore, ...

In this paper, we propose and demonstrate a novel self-referencing surface plasmon resonance (SPR) fiber-optic sensor which provides a Fabry-Perot (FP) interference referencing signal for temperature compensating. The sensor is fabricated by splicing a capillary partly coated with gold film between multimode fibers. The multimode fibers act as the lead-in and lead-out fibers while the capillary is used as sensing element. Because the FP interference and SPR effects can occur in the capillary simultaneously, the spectrum of the sensor exhibits SPR absorption and FP interference fringes. Due to the FP interference fringe sensitive to temperature while insensitive to refractive index (RI), it can be used as referencing signal and the SPR absorption was used as measuring signal. Experimental results show that this approach we presented can compensate temperature effect and develop this sensor as a practicable high-sensitivity sensing device. Moreover, as a self-referencing fiber-optic SPR sensor, ...

Diarrhoea caused by enterotoxigenic Escherichia coli is one of the leading causes of mortality in children under five years of age and is a great burden on developing countries. The major virulence factor of the bacterium is the heat-labile enterotoxin (LT), a close homologue of the cholera toxin. The toxins bind to carbohydrate receptors in the gastrointestinal tract, leading to toxin uptake and, ultimately, to severe diarrhoea. Previously, LT from human- and porcine-infecting ETEC (hLT and pLT, respectively) were shown to have different carbohydrate-binding specificities, in particular with respect to N-acetyllactosamine-terminating glycosphingolipids. Here, we probed eleven single-residue variants of the heat-labile enterotoxin with surface plasmon resonance spectroscopy and compared the data to the parent toxins. In addition we present a 1.45 Å crystal structure of pLTB in complex with branched Lacto-N-neohexaose (Galbeta4GlcNAcbeta6[Galbeta4GlcNAcbeta3]Galbeta4Glc). The largest difference in

An integrated surface plasmon resonance sensor Welcome to the Integrated Sensor System Training Program Website. Sensing is inherently a multidisciplinary field, and those who seek to advance and exploit knowledge in this area have a wide range of backgrounds, including physics, chemistry, mechanical and electrical engineering, biomedical engineering and medicine. Although often well-trained in their own domain of expertise they are not always ready to transfer their skills to industry. Most students have little exposure to other related research domains and do not appreciate that sensors are complex systems, requiring biorecognition or chemical recognition elements, sample delivery components (e.g. microfluidics), transducing elements, signal amplification, signal processing and packaging, and that a multidisciplinary approach is essential. Sensor systems must address specific markets and are subject to regulatory control. Students often also lack knowledge in the key techniques of micro or nano

1. V. Ntziachristos, Fluorescence molecular imaging, Annu. Rev. Biomed. Eng. 8(1), 1-33 (2006). [CrossRef] [PubMed] 2. S. Keren, C. Zavaleta, Z. Cheng, A. de la Zerda, O. Gheysens, and S. S. Gambhir, Noninvasive molecular imaging of small living subjects using Raman spectroscopy, Proc. Natl. Acad. Sci. U.S.A. 105(15), 5844-5849 (2008). [CrossRef] [PubMed] 3. J. Stevens and W. Baier, Refractometric determination of soluble solids in citrus juices, Ind. Eng. Chem. Anal. Ed. 11(8), 447-449 (1939). [CrossRef] 4. J. Homola, S. S. Yee, and G. Gauglitz, Surface plasmon resonance sensors: review, Sens. Actuators B Chem. 54(1-2), 3-15 (1999). [CrossRef] 5. G. Popescu, Quantitative Phase Imaging of Cells and Tissues (McGraw-Hill Professional, 2011).. 6. B. Kemper and G. von Bally, Digital holographic microscopy for live cell applications and technical inspection, Appl. Opt. 47(4), A52-A61 (2008). [CrossRef] [PubMed] 7. B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. ...

Plural circuit selection using role reversing control inputs | SYSTEM AND METHOD OF DATA COMMUNICATION USING TRELLIS CODED MODULATION OR TURBO TRELLIS CODED MODULATION IN... | Multiple bit memory cells and methods for reading non-volatile data | Two-dimensional blazed MEMS grating | Surface plasmon resonance sensor having real-time referencing |

TY - JOUR. T1 - Asp271 is critical for substrate interaction with the surface binding site in β-agarase A from Zobellia galactanivorans. AU - Wilkens, Casper. AU - Tiwari, Manish K.. AU - Webb, Helen. AU - Jam, Murielle. AU - Czjzek, Mirjam. AU - Svensson, Birte. PY - 2019. Y1 - 2019. N2 - In the marine environment agar degradation is assured by bacteria that contain large agarolytic systems with enzymes acting in various endo- and exo-modes. Agarase A (AgaA) is an endo-glycoside hydrolase of family 16 considered to initiate degradation of agarose. Agaro-oligosaccharide binding at a unique surface binding site (SBS) in AgaA from Zobellia galactanivorans was investigated by computational methods in conjunction with a structure/sequence guided approach of site-directed mutagenesis probed by surface plasmon resonance binding analysis of agaro-oligosaccharides of DP 4-10. The crystal structure has shown that agaro-octaose interacts via H-bonds and aromatic stacking along 7 subsites (L through R) of ...

Fcgamma receptors (FcgammaRs) are expressed on all immunologically active cells. They bind the Fc portion of IgG, thereby triggering a range of immunological functions. We have used surface plasmon resonance to analyze the kinetic and thermodynamic properties of the interactions between the ectodomains of human low affinity FcgammaRs (FcgammaRIIa, FcgammaRIIb, and FcgammaRIIIb-NA2) and IgG1 or the Fc fragment of IgG1. All three receptors bind Fc or IgG with similarly low affinities (K(D) approximately 0.6-2.5 microm) and fast kinetics, suggesting that FcgammaR-mediated recognition of aggregated IgG and IgG-coated particles or cells is mechanistically similar to cell-cell recognition. Interestingly, the Fc receptors exhibit distinct thermodynamic properties. Whereas the binding of the FcgammaRIIa and FcgammaRIIb to Fc is driven by favorable entropic and enthalpic changes, the binding of FcgammaRIII is characterized by highly unfavorable entropic changes. Although the structural bases for these

TY - JOUR. T1 - Investigation of the mechanism of binding between internalin B and heparin using surface plasmon resonance. AU - Hrtska, Sybil C Lang. AU - Kemp, Melissa M.. AU - Muñoz, Eva M.. AU - Azizad, Omaira. AU - Banerjee, Mani. AU - Raposo, Catarina. AU - Kumaran, Jyothi. AU - Ghosh, Partho. AU - Linhardt, Robert J.. PY - 2007/3/13. Y1 - 2007/3/13. N2 - Listeria monocytogenes, a food-borne pathogen that infects immunocompromised patients, enters and proliferates within mammalian cells by taking advantage of host cell machinery. While entry into macrophages and other phagocytic cells occurs constitutively, intracellular invasion of nonphagocytic cells, such as epithelial and endothelial cells, occurs through induced phagocytosis. Invasion of these nonphagocytic cell types is under the control of the secreted L. monocytogenes protein internalin B (InlB), which directly associates with and activates the receptor tyrosine kinase Met. Activation of Met by InlB has previously been shown to be ...

Biosensor technology is a powerful alternative to conventional techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. Despite the promising biosensors developed in research laboratories, there are not many reports of applications in agricultural monitoring. The authors review biosensor technology and discuss the different bio-receptor systems and methods of transduction. The difference between a biosensor and a truly integrated biosensor system are defined and the main reasons for the slow technology transfer of biosensors to the marketplace are reported. Biosensor research and development has been directed mainly towards health care, environmental applications and the food industry. The most commercially important application is the hand-held glucose meter used by diaberics. The agricultural/veterinary testing market has seen a number of diagnostic tests but no true biosensor systems have made an impact. The need for fast, on-line and accurate ...

Nanoplasmonic sensors typically comprise arrangements of noble metal nanoparticles on a dielectric support. Thus they are intrinsically characterized by surface topography with corrugations at the 10-100 nm length scale. While irrelevant in some bio- and chemosensing applications, it is also to be expected that the surface topography significantly influences the interaction between solids, fluids, nanoparticles and (bio)molecules, and the nanoplasmonic sensor surface. To address this issue, we present a wafer-scale nanolithography-based fabrication approach for high-temperature compatible, chemically inert and topographically flat and laterally homogeneous nanoplasmonic sensor chips. We demonstrate their sensing performance on three different examples, for which we also carry out a direct comparison with a traditional nanoplasmonic sensor with representative surface corrugation. Specifically, we (i) quantify the film-thickness dependence of the glass transition temperature in poly(methyl metacrylate)

This paper reports the fabrication and testing of two configurations of optical sensor systems based on Surface Plasmon Resonance (SPR) at the interface of a liquid sample and sandwiched structures realized starting from the exposed core of a Plastic Optical Fiber (POF). The proposed geometries have proven to be suitable for measuring the refractive indexes of liquids whose refractive index falls around 1.35. Furthermore, the proposed sensing head, being low cost and relatively easy to realize, may be very attractive for biosensor implementation.

Angiopoietin-like 4 (ANGPTL4) is involved in angiogenesis and lipid metabolism. It is secreted by liver and adipose tissues and cleaved to generate circulating coiled-coil domain (CCD) and fibrinogen-like domain (FLD) fragments. The full-length ANGPTL4 produced by hypoxic endothelial cells interacts with the extracellular matrix (ECM). The ECM-bound and soluble forms of ANGPTL4 have antiangiogenic properties. We carried out a structure-function analysis to investigate the regulation of ANGPTL4 bioactivity in endothelial cells. We found that the recombinant CCD binds to the ECM, whereas the FLD is released into the medium. The CCD, like the full-length ANGPTL4, binds to heparan and dermatan sulfates in surface plasmon resonance assays and inhibits endothelial cell adhesion, motility, and tubule-like formation. In endothelial cells, ANGPTL4 is processed in the secretion medium after release from the ECM. This processing is altered by the proprotein convertases inhibitor alpha1-PDX and abolished by the

The purpose of this work was to study the effect of various process and formulation parameters on size and shapes of silver nanoparticles (AgNPs) based on surface plasmon resonance (SPR). AgNPs were prepared by chemical reduction using formaldehyde (HCHO) as reducing agent and capped by polyethylene glycol (PEG). Effect of several processing variables including the concentration and volume of capping agent and reaction time is reported. The size of monodispersed nanoparticles was between 30-100 nm and was stable for three months at both room temperature and 4°C ...

In this study, we investigated the plasmon resonances of split-ring resonators (SRRs) numerically at incident angles of 0 and 45° under illumination with linearly and circularly polarized waves. At 45° incidence, perpendicular polarized waves excited distinct odd plasmon modes; the difference in the reflections of right and left circularly polarized incident radiation was very large. From simulated near field plots, we found that the parallelism of the incident electric field and the induced plasmon current was the key factor affecting excitation. We propose the use of a parallelism factor (P-factor), based on a standing-wave approach, to characterize the ability of incident fields to excite multiple plasmon resonance currents. The mechanism of the field and current parallelism can explain the resonance behavior of SRRs when considering the polarization state, incident angle, and geometry of the SRR.. ©2010 Optical Society of America. Full Article , PDF Article ...

A novel biosensing approach for the label-free detection of nucleic acid sequences of short and large lengths has been implemented, with special emphasis on targeting RNA sequences with secondary structures. The approach is based on selecting 8-aminoadenine-modified parallel-stranded DNA tail-clamps as affinity bioreceptors. These receptors have the ability of creating a stable triplex-stranded helix at neutral pH upon hybridization with the nucleic acid target. A surface plasmon resonance biosensor has been used for the detection. With this strategy, we have detected short DNA sequences (32-mer) and purified RNA (103-mer) at the femtomol level in a few minutes in an easy and level-free way. This approach is particularly suitable for the detection of RNA molecules with predicted secondary structures, reaching a limit of detection of 50fmol without any label or amplification steps. Our methodology has shown a marked enhancement for the detection (18 for short DNA and 54 for RNA), when compared ...

OpenPlex, Horiba Scientifics redesigned manual label-free SPRi-Lab+ system, uses surface plasmon resonance imaging (SPRi) for real-time analysi

A Rutgers-led team has created better biosensor technology that may help lead to safe stem cell therapies for treating Alzheimers and Parkinsons diseases and other neurological disorders. The technology, which features a unique graphene and gold-based platform and high-tech imaging, monitors the fate of stem cells by detecting genetic material (RNA) involved in turning such cells into brain cells (neurons), according to a study in the journal Nano Letters.

Nanoplasmonics has raised much concern on tailoring the strong optical fields near noble metal nanostructures. In particular, surface-enhanced Raman Scattering (SERS) can take the full benefits of nanoplasmonics for providing one of the most highly sensitive biochemical sensing techniques. This sensitivity is used to identify biochemical molecules even at single molecule level. This technique does not require fluorescent labeling. For decades, thousands of plasmonic nanoparticles or nanostructures mounted on substrates have been reported for plasmonics-driven SERS. However, previous work overlooks consideration of the quantitative investigation between plasmon resonance and SERS signals. This oversight has been due to available methodology.. Universal correlations between plasmon resonance and SERS has recently been described by the research of Ki-Hun Jeong (see, Kang et al.1) in Journal Advanced Materials - This observation was successfully enabled by deformable nanoplasmonic membrane, i.e., ...

The αβ T-cell coreceptor CD4 enhances immune responses more than 1 million-fold in some assays, and yet the affinity of CD4 for its ligand, peptide-major histocompatibility class II (pMHC II) on antigen-presenting cells, is so weak that it was previously unquantifiable. Here, we report that a soluble form of CD4 failed to bind detectably to pMHC II in surface plasmon resonance-based assays, establishing a new upper limit for the solution affinity at 2.5 mM. However, when presented multivalently on magnetic beads, soluble CD4 bound pMHC II-expressing B cells, confirming that it is active and allowing mapping of the native coreceptor binding site on pMHC II. Whereas binding was undetectable in solution, the affinity of the CD4/pMHC II interaction could be measured in 2D using CD4- and adhesion molecule-functionalized, supported lipid bilayers, yielding a 2D Kd of ∼5,000 molecules/μm(2) This value is two to three orders of magnitude higher than previously measured 2D Kd values for interacting

The obligate human pathogen Neisseria gonorrhoeae is responsible for the widespread sexually transmitted disease gonorrhoea, which in rare cases also leads to the development of disseminated gonococcal infection (DGI). DGI is mediated by PorBIA-expressing bacteria that invade host cells under low phosphate condition by interaction with the scavenger receptor-1 (SREC-I) expressed on the surface of endothelial cells. The interaction of PorBIA and SREC-I was analysed using different in vitro approaches, including surface plasmon resonance experiments that revealed a direct phosphate-independent high affinity interaction of SREC-I to PorBIA. However, the same binding affinity was also found for the other allele PorBIB, which indicates unspecific binding and suggests that the applied methods were unsuitable for this interaction analysis. Since N. gonorrhoeae was recently classified as a super-bug due to a rising number of antibiotic-resistant strains, this study aimed to discover inhibitors against ...

Generally, an immunoaffinity SPR biosensor detects a target analyte in a sample through highly selective adsorption by using the antigen–antibody interaction. For improving the sensitivity, various kinds of particles have been added to the already bound analytes on the SPR biosensor (sandwich assay). In this work, signal amplification was demonstrated by the expression of the IgG-binding Z-domain of protein A on the outer membrane of Escherichia coli via Autodisplay. The amount of Z-domain of protein A expressed on the outer membrane was calculated to be 280,000 molecules per cell. In addition, the IgGbinding ability of the expressed proteinwas characterized using FACS analysis. The signal amplification of the SPR biosensor was performed in the sandwich assay format using a model of horseradish peroxidase (HRP); the limit of detectionwas determined to be significantly improved from1 g/ml to 1 ng/ml. Finally, myoglobin analysis was demonstrated for the medical diagnosis of cardiac ...

Analysis of biological components is central in bioprocess monitoring, process control, product quality control and cell based toxicity assaying. One of these themes that is pursued in this thesis is the use of biosensors for monitoring of molecular markers, exploiting the natural selectivity of biomolecules. Another is the use of glycoconjugates to monitor the activity of biomolecules in a flu vaccine process is studied and were the sensor is based on the concept of weak affinity giving fast response time for the sensor.. A third theme is monitoring of cell cultures used for toxicity testing different protein markers is of interest.. When developing biosensor surfaces for new antigens commercial preparations of antibodies are often used. In this work we have chosen to look at lactate dehydrogenase (LDH) and describe the preparation and characterisation of antibody used in biosensor surface development.. The design of a sensor surface is important for the characteristics of a sensor. By binding ...

In order to overcome the sensitivity limitation of SPR, nanoparticle-coupled SPR biosensors have explored since nanoparticles may significantly enhance the sensitivity by 1-2 orders of magnitude. Although the nanoparticle conjugated method contributes to the enhancement of the sensitivity, it does not take an advantage of SPR from the viewpoint of label-free detection. Herein we demonstrate the sensitivity enhancement with Au nanoparticles coupled- SPR immuno-sensor chip on which the specific size and surface density of the particles controlled as a label-free detection system. Au nanopariticles were synthesized and selected with a specific size. Surface density of immobilized 30 nm Au nanoparticles on bare Au film was estimated as 1x109 ea/cm2 using atomic force microscopy. With systematic control of the size of the Au nanoparticle and thickness of the bare Au film, it was found that 30 nm Au particles on 50 nm thick Au film demonstrated the largest resonance angle shift for surface reaction on the

TY - JOUR. T1 - Toward surface plasmon polariton quantum-state tomography. AU - Dominguez, D.. AU - Regan, C. J.. AU - Bernussi, A. A.. AU - Grave De Peralta, L.. PY - 2013/2/21. Y1 - 2013/2/21. N2 - We report the direct excitation and detection of single-photon surface plasmon polariton (SPP) using a SPP tomography arrangement. Temporally spaced photons produced by spontaneous parametric downconversion were used to excite single-photon SPPs. The quantum statistics of the leakage radiation was studied using a Hanbury-Brown Twiss correlator arrangement. We observed a violation of the second order coherence test indicating leakage of temporally spaced photons. This demonstrates that leakage radiation associated with SPPs excited by single photons is composed of temporally spaced photons. Reaching the quantum regime of SPP tomography opens the door for further advances in SPP quantum state determination using SPP tomography.. AB - We report the direct excitation and detection of single-photon ...

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International Journal of Electrochemistry is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles that further our understanding of fundamental electrochemical processes, describe new electrochemical techniques, apply electrochemistry in analytical determination, or apply electrochemistry for chemical reactivity.

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Get this from a library! Narrow plasmon resonances in hybrid systems. [Philip A Thomas] -- Advances in understanding the interactions between light and subwavelength materials have enabled the author and his collaborators to tailor unique optical responses at the nanoscale. In particular, ...

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Mentors: Berron, Hastings. Surface-plasmon resonance (SPR) sensing is a widely used optical technique for detecting and analyzing biochemical interactions. It has found applications in diverse fields such as medical diagnostics, drug discovery, and environmental monitoring. A key challenge for SPR is maximizing sensitivity to a target analyte while minimizing response to interfering species. Dr. Hastings group has developed several SPR sensors that use optical methods to distinguish specific and non-specific interactions. Dr. Berrons group has developed stable, low-density self-assembled monolayers that are ideal for functionalizing the gold surfaces common to SPR sensors. The tunable density of exposed functional groups should allow optimization of the interaction with a chosen target, while suppressing non-specific protein binding. In addition, the SPR technique itself will increase our understanding of the kinetics of protein adsorption on low-density monolayers. Students working on this ...

TY - JOUR. T1 - The interaction of serum albumin with cholesterol containing lipid vesicles. AU - Meierhofer, T. AU - van den Elsen, J M H. AU - Cameron, Petra J. AU - Munoz-Berbel, X. AU - Jenkins, A T A. PY - 2010/1. Y1 - 2010/1. N2 - In this paper, the interaction of both human blood serum (the primary fraction of which is serum albumin) and pure human serum albumin (HSA) with surface immobilised lipid vesicles was measured by combined Surface Plasmon Resonance (SPR) and Surface Plasmon enhanced Fluorescence (SPEFS), and fluorescence microscopy. It was found that both blood serum and HSA showed specific binding to vesicles which contained cholesterol, resulting in increased membrane permeability and release of encapsulated fluorescent dye. This effect was not seen with heat inactivated blood serum, heat inactivated HSA or in vesicles not containing cholesterol. These results suggest that HSA may have a physiological role over and beyond that of fatty acid carrier, possibly acting to regulate ...

Biomolecular interaction analysis (BIA) using SPR (surface plasmon resonance) biosensors is now utilised increasingly in nearly all phases of drug development. The BIA system consists out of a light source emitting near infrared light, a sensor microchip, an automated liquid handling system with constant flow and a diode array position-sensitive detector. One of the two interacting partners (referred to as the ligand) is immobilized on the sensor surface. The other binding partner, called the analyte, is directed over the surface in a constant flow system allowing to monitor the interaction of the binding partners in real time ...

The production of botulinum neurotoxin A (BoNT/A) for therapeutic and cosmetic applications requires precise determination of batch potency, and the enzymatic activity of BoNT/A light chain is a crucial index that can be measured in vitro. We previously established a SNAP-25 chip-based assay using surface plasmon resonance (SPR) that is more sensitive than the standard mouse bioassay for the quantification of BoNT/A activity. We have now adapted this procedure for pharmaceutical preparations. The optimized SPR assay allowed multiple measurements on a single chip, including the kinetics of substrate cleavage. The activity of five different batches of a pharmaceutical BoNT/A preparation was determined in a blind study by SPR and found to be in agreement with data from the in vivo mouse lethality assay. Biosensor detection of specific proteolytic products has the potential to accurately monitor the activity of pharmaceutical BoNT/A preparations, and a single chip can be used to assay more than 100 ...

Profacgen provides One-Stop-Service on protein-protein interaction analysis, including Yeast two-hybrid, Pull-downs and Surface Plasmon Resonance (SPR) assay etc., to facilitate your scientific research. Our service can be tailored according to your specific requirements.

Our laboratory improves magnetic resonance technology and spectroscopy for biomolecular structure determination relevant to HIV cure research. Solid state nuclear magnetic resonance (NMR) is exquisitely suited to probe atomic level structural detail of biomolecules within intact human cells. However, the small nuclear magnetic moments that yield narrow resonances also result in very low inherent sensitivity in NMR. Dynamic Nuclear Polarization (DNP) is a powerful combined electron paramagnetic resonance (EPR) and NMR technique that transfers the strong polarization from unpaired electron spins to nuclear spins to boost NMR sensitivity. In our DNP experiments, we employ frequency agile gyrotrons, and extreme cryogenic sample cooling to increase the sensitivity of solid state NMR experiments up to a factor of 20,000. This tremendous gain in sensitivity results in acquiring data 400 million times faster than conventional NMR experiments and will have a profound impact on magnetic resonance ...

Waltham, MA, January 2011 - Nova Biomedical today announced that, in response to rapid growth in its diabetes and whole blood point-of-care testing products business, it has purchased an additional 80,000 square-foot manufacturing/warehouse facility in Billerica, MA. According to Lou Borrelli, Nova Biomedical CFO, This additional state of the art manufacturing facility will ensure that our manufacturing capabilities keep pace with the increasing demand for our StatStrip Hospital Glucose products as well as our Nova Max consumer diabetes products.. One of the main drivers for Novas strong growth is the rapid adoption of its StatStrip Hospital Glucose Monitoring System. Since its inception just four years ago, StatStrip has become the fastest growing hospital glucose meter in the world. StatStrip uses a novel glucose test strip technology that measures hematocrit and other common interferences such as maltose, galactose, xylose, acetaminophen, ascorbic acid and oxygen, and eliminates erroneous ...

The most rapid and reliable way of detecting HIV is to search for the HIV genetic material directly in blood, using a process called PCR (polymerase chain reaction). This has the advantage of not needing to wait for such a long time after exposure, being used after only 3 days. Traditionally this process, known as molecular diagnostics, has been performed in a laboratory by trained personnel. Recent technological advances have however brought the possibility of conducting molecular diagnostics out of the laboratory and next to the patient at the point-of-care ...

1. A method for obtaining the binding kinetic rate constants using a fiber optic particle plasmon resonance (FOPPR) sensor, comprising the steps of: providing a fiber optic particle plasmon resonance sensor, wherein the fiber optic particle plasmon resonance sensor at least comprises: a light source to emit a light beam; a photoreceiver; and a fiber sensor chip, wherein the fiber sensor chip is located between the light source and the photoreceiver and the fiber sensor chip comprises: an optical fiber, wherein the optical fiber comprises a first region and a second region; the first region is located at two corresponding sides of the second region, wherein the first region comprises a fiber core, a cladding, and a protective layer, the refractive index of the fiber core is greater than that of the cladding such that the light beam can propagate within the fiber core; and the second region comprises the fiber core, the cladding, a noble metal nanoparticle layer, and a bio-recognition layer; a ...

article{a2ab6767-ca6c-4527-8f1e-217da06cea72, abstract = {Nontypeable Haemophilus influenzae (NTHi) commonly causes local disease in the upper and lower respiratory tract and has recently been shown to interfere with both the classical and alternative pathways of complement activation. The terminal pathway of the complement system is regulated by vitronectin that is a component of both plasma and the extracellular matrix. In this study, we identify protein E (PE; 16 kDa), which is a recently characterized ubiquitous outer membrane protein, as a vitronectin-binding protein of NTHi. A PE-deficient NTHi mutant had a markedly reduced survival in serum compared with the PE-expressing isogenic NTHi wild type. Moreover, the PE-deficient mutant showed a significantly decreased binding to both soluble and immobilized vitronectin. In parallel, PE-expressing Escherichia coli bound soluble vitronectin and adhered to immobilized vitronectin compared with controls. Surface plasmon resonance technology ...

A panel of 19 monoclonal antibodies (mAbs) was used to study the immunological variability of Lettuce mosaic virus (LMV), a member of the genus Potyvirus, and to perform a first epitope characterization of this virus. Based on their specificity of recognition against a panel of 15 LMV isolates, the mAbs could be clustered in seven reactivity groups. Surface plasmon resonance analysis indicated the presence, on the LMV particles, of at least five independent recognition/binding regions, correlating with the seven mAbs reactivity groups. The results demonstrate that LMV shows significant serological variability and shed light on the LMV epitope structure. The various mAbs should prove a new and efficient tool for LMV diagnostic and field epidemiology studies.. ...

A biomimetic approach was taken for studying the adsorption of a model copolymer (pullulan abietate, DS 0.027), representing the lignin-carbohydrate complex, to a model surface for cellulose fibers (Langmuir-Blodgett thin films of regenerated cellulose). Adsorption results were assayed using surface plasmon resonance spectroscopy (SPR) and atomic force microscopy (AFM). Rapid, spontaneous, and desorption-resistant surface modification resulted. This effort is viewed as a critical first step towards the permanent surface modification of cellulose fibers with a layer of molecules amenable to either enzymatic crosslinking for improved wood composites or thermoplastic consolidation.

0169] For example, in one aspect, a structure may be formed, wherein a waveguide layer, in which a thin layer is planar, is located on a base material (e.g., Pyrex glass (trade name)). The waveguide layer and the base material together form the so-called waveguide body. The waveguide layer may be constituted of a laminate of a plurality of layers, such as oxide layers (e.g., SiO2, SnO2, Ta2O5, TiO2, TiO2--SiO2, HfO2, ZrO2, Al2O3, Si3N4, HfON, SiON, scandium oxide, and mixtures of the above-enumerated oxides) and plastic layers (e.g., a polystyrene, a polyethylene, and a polycarbonate). In order for a light beam to propagate in the waveguide layer through the total reflection, it is necessary that the refractive index of the waveguide layer is larger than the refractive index of the adjacent medium (such as the base material or the additional layer, which will be described later). A diffraction grating is located in the waveguide layer surface or the waveguide volume, which stands facing the base ...

article{6b8e8868-1afd-41d6-808d-4fc5532ec3af, abstract = {The ability of the leucine-rich repeat (LRR) proteins biglycan, decorin and chondroadherin to interact with collagen VI and influence its assembly to supramolecular structures was studied by electron microscopy and surface plasmon resonance measurements in the BIAcore 2000 system. Biglycan showed a unique ability to organize collagen VI into extensive hexagonal-like networks over a time period of only a few minutes. Only the intact molecule, substituted with two dermatan sulfate chains, had this capacity. Intact decorin, with one dermatan sulfate chain only, was considerably less efficient and aggregates of organized collagen VI were found only after several hours. Chondroadherin without glycosaminoglycan substitutions did not induce any ordered collagen VI organization. However, all three related LRR proteins were shown to interact with collagen VI using electron microscopy and surface plasmon resonance. Biglycan and decorin were ...

An immunosensor assay for the quantitation of intracellular recombinant human superoxide dismutase (rhSOD) in Escherichia coli cultivations based on detection with surface plasmon resoance (SPR) is described. A monoclonal antibody for rhSOD was immobilized on a SPR dextran gold chip. Bacterial samples were sonicated and centrifugated prior to injection over the antibody chip for SPR detection. The assay time was 7min and allowed quantitation in the range of 1-64nM SOD in lysate samples with a precision of 1.1-3.4%. The assay was applied to monitor the concentration of rhSOD during E. coli bioreactor cultivations where the rhSOD production was induced by iso-propyl-b-D-thiogalactoside (IPTG). The assay allowed accurate monitoring of the production of rhSOD where the important phases in the product formation were possible to see. The report also discusses influence from sample preparation, SPR selectivity and sensitivity and quantitation limits. The assay proved to be fast, sensitive and accurate ...

Surface plasmons are photo-coupled quanta of electro excitation at the boundary of a metal and dielectric, which is a charge density wave of free electrons. Another important feature of surface plasmons resonance, in addition to the enhancement of electric field, is the ability to pass through surface structure smaller than incident laser wavelength and makes it have a potential to overcome the diffraction limit resulting in the breakthrough of nanotechnology. For surface plasmon resonance developing a mask with small feature size is vital. In this paper, mask fabrication by laser irradiation on self-assembly silica particles was described. Compared with the theoretical simulation, it is shown that self-assembly particle-assisted nanopatterning can fabricate a mask with very fine features effectively. With a proper particle size, laser wavelength and laser fluence, periodical structures generated can meet the requirement for surface plasmon use. © 2007 Elsevier B.V. All rights reserved ...

Harmful algal blooms (HABs) occur worldwide, causing health problems and economic damages to fisheries and tourism. Monitoring agencies are therefore essential, yet monitoring is based only on time-consuming light microscopy, a level at which a correct identification can be limited by insufficient morphological characters. The project MIDTAL (Microarray Detection of Toxic Algae)-an FP7-funded EU project-used rRNA genes (SSU and LSU) as a target on microarrays to identify toxic species. Furthermore, toxins were detected with a newly developed multiplex optical Surface Plasmon Resonance biosensor (Multi SPR) and compared with an enzyme-linked immunosorbent assay (ELISA). In this study, we demonstrate the latest generation of MIDTAL microarrays (version 3) and show the correlation between cell counts, detected toxin and microarray signals from field samples taken in Arcachon Bay in France in 2011. The MIDTAL microarray always detected more potentially toxic species than those detected by microscopic counts

Lead Researcher: Dr Katrina Campbell. For more than a decade the Advanced Asset laboratory has been pioneering the use of biosensor technology in the area of food safety monitoring and we are recognised as world-leading research group in this area. We are experts in the development of highly innovative sensor based detection systems relating to biological and chemical contaminants in foods of pertinence to food integrity, safety, defence, quality and sustainability. Examples of such contamination include: naturally occurring toxins of fungal, algal, plant and bacterial origin present in the environment (e.g. mycotoxins and marine biotoxins); improper use of agrichemicals (e.g. antibiotics, pesticides), the illegal use of growth promoting compounds in animal production (e.g. B-agonists), by-products from food processing (e.g. acrylamide, allergens) and food-borne pathogens.. In addition to the traditional enzyme and luminescence based immunoassay design capabilities (e.g. ELISA and LFDs) the ...

... BEIJING J... The study is entitled A Surface Plasmon Resonance-based System t... The Company also announced the creation of a new column MedicalP...,China,Medical,Technologies,Announces,the,Recent,Publication,of,a,Study,on,its,SPR,Analyzer,and,HPV,DNA,Chip,in,Cancer,Genetics,and,Cytogenetics,and,the,Creation,of,a,Medical,Publications,Column,to,the,Investor,Relations,Section,on,its,Website,medicine,advanced medical technology,medical laboratory technology,medical device technology,latest medical technology,Health

Synthetic peptides incorporating the complementarity determining region 2 (CDR2)-like loop of domain 1 of human polymeric immunoglobulin receptor (hpIgR-D1) were investigated for their interactions with human Immunoglobulin-M (hIgM) and other hIgs. pep14, a 14mer peptide, emerged as a unique biomimetic ligand with specificity to interact with hIgM and negligible affinity for hIgG, hIgE, hIgA1 and bovine serum albumin. Surface plasmon resonance-based binding studies between immobilized pep14 and fragments of hIgM molecule, namely Fc5μ and Fab, suggested that pep14 was binding to a motif in the constant domain 2 of hIgM. Modified peptides, pep13, pep5A and pep14A were investigated for their interaction with hIgM/ hIgG1 to elucidate the binding mechanism of pep14. Cysteine in pep14 was identified to be crucial for inducing thiophilic-like interactions between the ligand and hIgM while glutamic acid had a significant role in attributing specificity to the ligand to interact with hIgM. Circular ...

The interactions of B7-1 with CD28 and CTLA-4 modulate the course of human immune responses, making B7-1 an important target for developing structure-based therapeutics. B7-1 is, however, one of the most heavily glycosylated proteins found at the leukocyte cell surface, complicating the structural analysis of this molecule. Methods for the production, crystallization and selenomethionine labelling of a soluble deglycosylated form of this molecule are described. The protein readily forms both tetragonal plate and bipyramidal crystals belonging to space groups I4(1)22, with unit-cell parameters a = b = 56.9, c = 298.7 A, and P4(1)22 (or P4(3)22), with unit-cell parameters a = b = 89.0, c = 261.9 A, respectively. The I4(1)22 and primitive crystal forms diffract to 2.7 and 3.5 A, respectively. Surface plasmon resonance-based assays indicate that the ligand-binding properties of sB7-1 are unaffected by deglycosylation. Since none of the methods relied on any special structural properties of sB7-1, it is

volleyball training centre,volleyball court qatar,free live cricket app for pc,Surface plasmon resonance is an optical phenomenon. Surface plasmon resonance (SufacpasonsonancSP) refers to a physical phenomenon in which surface plasmons are generated by coherent oscillation of free electrons on the metal surface when the incident light field meets a certain energy and momentum matching condition at the interface of metal and dielectric.

The preparation of a new poly(thiophene) with pendant N-hydroxysuccinimide ester groups and its application to immobilization of biomolecules are reported. A thiophene derivative of N-hydroxysuccinimide ester was polymerized with FeCl3 in chloroform and the resulting poly(thiophene) was characterized by nuclear magnetic resonance (NMR), Fourier transform infrared (FT-IR), and gel permeation chromatography (GPC). This polymer reacts with amine-bearing molecules to yield new poly(thiophene) derivatives and the specific interactions at the side groups could be detected. Thus, a self-assembled monolayer (SAM) using the polymer was formed on a gold-coated quartz cell and anti-C-reactive protein (anti-CRP) was immobilized. The binding behavior of CRP on the surface was monitored by use of a surface plasmon resonance (SPR) sensor system. © 2008 Elsevier B.V. All rights reserved. ...

Diamond like carbon films containing silicon (DLC:SiOx) and „conventional hydrogenated diamond like carbon (DLC) films were deposited by direct ion beam using anode layer ion source. Ag films were grown by unbalanced direct current magnetron sputtering. Structure of DLC:SiOx films was investigated by Raman scattering spectroscopy. In the case of DLC:SiOx film deposited on Ag layer surface enhanced Raman scattering effect was observed. Optical properties of the different diamond like carbon and silver multilayers were studied. Annealing effects were investigated. Influence of the thickness of the diamond like carbon and Ag layers was investigated. Position of the plasmonic absorbance peak maximum of DLC:SiOx and multilayers in all cases was redshifted in comparison with conventional diamond like nanocomposite films containing silver nanoclusters. It was explained by increase of the Ag nanoparticle size and/or increased probability of the oxidation of the embedded Ag due to the higher amount ...

TY - JOUR. T1 - A gram-negative characteristic segment in Escherichia coli DnaK is essential for the ATP-dependent cooperative function with the co-chaperones DnaJ and GrpE. AU - Sugimoto, Shinya. AU - Higashi, Chihana. AU - Saruwatari, Kozue. AU - Nakayama, Jiro. AU - Sonomoto, Kenji. PY - 2007/6/26. Y1 - 2007/6/26. N2 - We describe importance of the characteristic segment in ATPase domain of DnaK chaperone which is present in all gram-negative bacteria but is absent in all gram-positive bacteria. In vitro studies, ATPase activity, luciferase-refolding activity, and surface plasmon resonance analyses, demonstrated that a segment-deletion mutant DnaKΔ74-96 became defective in the cooperation with the co-chaperones DnaJ and GrpE. In addition, in vivo complementation assay showed that expression of DnaKΔ74-96 could not rescue the viability of Escherichia coli ΔdnaK mutant at 43 °C. Consequently, we suggest evolutionary significance for this DnaK ATPase domain segment in gram-negative bacteria ...

Cells inhibitor of metalloproteinases-1 (TIMP-1) regulates the extracellular matrix Genkwanin turnover by inhibiting the proteolytic activity of matrix metalloproteinases (MMPs). chips for surface plasmon resonance analysis. Main cortical neurons bound and internalized endogenous TIMP-1 through a mechanism mediated by LRP-1. This resulted in inhibition of neurite outgrowth and improved growth cone volume. Using a mutated inactive Genkwanin TIMP-1 variant we showed that TIMP-1 effect on neurone morphology was self-employed of its MMP inhibitory activity. We conclude Genkwanin that TIMP-1 is definitely a new ligand of LRP-1 and we spotlight a new example of its MMP-independent cytokine-like functions. Intro The four cells inhibitors of metalloproteinases Genkwanin (TIMP-1-4) inhibit the proteolytic activity of matrix metalloproteinases (MMPs) and collectively constitute the principal regulators of the pericellular environment in physiological and pathological situations [1]. Individually of their ...

Growth and Differentiation Factor 5 (GDF5) is a secreted growth factor that belongs to the Bone Morphogenetic Protein (BMP) family and plays a pivotal role during limb development. GDF5 is a susceptibility gene for osteoarthritis (OA) and mutations in GDF5 are associated with a wide variety of skeletal malformations ranging from complex syndromes such as acromesomelic chondrodysplasias to isolated forms of brachydactylies or multiple synostoses syndrome 2 (SYNS2). Here, we report on a family with an autosomal dominant inherited combination of SYNS2 and additional brachydactyly type A1 (BDA1) caused by a single point mutation in GDF5 (p.W414R). Functional studies, including chondrogenesis assays with primary mesenchymal cells, luciferase reporter gene assays and Surface Plasmon Resonance analysis, of the GDF5 W-414R variant in comparison to other GDF5 mutations associated with isolated BDA1 (p.R399C) or SYNS2 (p.E491K) revealed a dual pathomechanism characterized by a gain-and loss-of-function at ...

The local surface plasmon resonance (LSPR) of noble metal nanoparticles has recently been exploited in numerous applications. The LSPR peak position and linewidth have been studied quite extensively, but the magnitude of the resonance has not received much attention. Analytical solutions to Maxwells Equations cannot predict the scattering cross section of arbitrarily-shaped particles at arbitrary illumination and detection angles. Dark field microscpectroscopy is a powerful tool for studying plasmon resonances of noble metal nanoparticles and for developing their applications in sensing and imaging. We present a technique for calibrating dark field microspectrometer measurements to yield quantitative spectral scattering cross sections for arbitrarily shaped particles. Values for gold nanorods and gold bipyramids are reported. The measurements suggest that, for small elongated particles, the signal can be predicted by approximations based on the total cross section ...

Determination of the Rituximab Binding Site to the CD20 Epitope Using SPOT Synthesis and Surface Plasmon Resonance Analyses, Bar, Laure, Nguyen Christophe, Galibert Mathieu, Santos-Schneider Francisco, Aldrian Gudrun, Dejeu Jérôme, Lartia Remy, Coche-Guérente Liliane, Molina Franck, and Boturyn Didier , Analytical Chemistry, 04/2021, Volume 93, Issue 17, p.6865−6872, (2021) ...

In this paper, the issues pertaining to moisture diffusion in PEMs are explored and discussed. The existing models of moisture diffusion in plastic molding

The neurotensin receptor 1 represents an important drug target involved in various diseases of the central nervous system. So far, the full exploitation of potential therapeutic activities has been compromised by the lack of compounds with favorable physicochemical and pharmacokinetic properties which efficiently penetrate the blood-brain barrier. Recent progress in the generation of stabilized variants of solubilized neurotensin receptor 1 and its subsequent purification and successful structure determination presents a solid starting point to apply the approach of fragment-based screening to extend the chemical space of known neurotensin receptor 1 ligands. In this report, surface plasmon resonance was used as primary method to screen 6369 compounds. Thereby 44 hits were identified and confirmed in competition as well as dose-response experiments. Furthermore, 4 out of 8 selected hits were validated using nuclear magnetic resonance spectroscopy as orthogonal biophysical method. Computational ...

Surface plasmon resonance (SPR) biosensors are used to characterize and quantify biomolecular interactions. Real-time and fast measurement, high sensitivity and specificity, no need of labeled reagents are the unique properties of SPR biosensors. SPR biosensors have been used for detection of several analytes. Recently, molecular imprinting technology is used for creation of biorecognition surfaces on the SPR biosensors. Molecular imprinting is the technology of designing materials with robust recognition sites suitable for interaction with the print molecule. Due to their stability in complex matrices under tough conditions, ease of preparation, low cost, reusability, high recognition capacity to the template molecule and binding characteristics as high as those of natural binders, molecularly imprinted polymers (MIPs) are extensively used in a variety of areas, such as catalysis, separations, solid-phase extractions and biosensors. In this study, an SPR biosensor was prepared for prostate ...

Nanoparticles of some metals (Cu/Ag/Au) sustain oscillations of their electron cloud called localized surface plasmon resonances (LSPRs). These resonances can occur at optical frequencies and be driven by light, generating enhanced electric fields and spectacular photon scattering. However, current plasmonic metals are rare, expensive, and have a limited resonant frequency range. Recently, much attention has been focused on earth-abundant Al, but Al nanoparticles cannot resonate in the IR. The earth-abundant Mg nanoparticles reported here surmount this limitation. A colloidal synthesis forms hexagonal nanoplates, reflecting Mgs simple hexagonal lattice. The NPs form a thin self-limiting oxide layer that renders them stable suspended in 2-propanol solution for months and dry in air for at least two week. They sustain LSPRs observable in the far-field by optical scattering spectroscopy. Electron energy loss spectroscopy experiments and simulations reveal multiple size-dependent resonances with ...

The interference pattern observed in Youngs double-slit experiment is intimately related to the statistical correlations of the waves emitted by the slits. As the waves in the slits become more correlated, the visibility of the interference pattern increases. Here, we experimentally modulate the statistical correlations between the optical fields emitted by a pair of slits in a metal film. The interaction between the slits is mediated by surface plasmon polaritons and can be tuned by the slit separation, which allows us to either increase or decrease the spatial coherence of the emerging fields relative to that of the incoming fields.. © 2016 Optical Society of America. Full Article , PDF Article ...

With unique surface plasmon resonance and high potential for many important applications, plasmonic nanostructures are attracting more and more attention in the past decade. In addition to ever increasing research interest in realizing precise control over their structure (such as size, shape, aspect ratio and uniformity), plasmon enhanced catalysis represents a highly promising research area. In this presentation, the development of several types of plasmonic nanostructures by wet chemical method or pulsed laser ablation will be introduced first. Their interesting optical properties and their applications in photocatalysis will then be presented and discussed ...

Localized surface plasmon resonances of gold nanorods are powerful in enhancing a variety of linear and nonlinear optical signals and photo-related processes. The plasmon-induced concentration of light onto the regions adjacent to gold nanorods can be utilized for enhancing the light scattered from dielectric particles or biological structures. By decorating a layer of gold nanoparticles onto the hollow silica microsphere, the scattering intensity of the microsphere can be enhanced up to 100 times. The scattering enhancing region can also be tuned by using gold nanoparticles with different localized plasmon resonances. Such an ability of gold nanoparticles could be utilized to enhance the scattering signals from biological cells in light scattering spectroscopy as well as improving the harvesting efficiency in solar cells.. ...

Surface plasmon biosensing technology for antimicrobial susceptibility test - References | InTechOpen, Published on: 2011-07-19. Authors: How Foo Chen, Chi-Hung Lin, Chun-Yao Su, et

One of the most important requirements for making advanced biosensors and biochips is the attachment of biological receptors onto a solid support. For this reason, it is important to construct a stable and well-characterized interface chemistry. Therefore the biosensor research at IMEC focuses on the use of (pre-activated) mixed self-assembled monolayers (SAMs) of alkane thiols on gold surfaces. Their most important characteristics were investigated. Firstly, we investigated their stability. To address this issue, the mixed SAMs were tested in several storage conditions in function of time. The quality was monitored not only with common characterization tools but also the biosensor properties were investigated in time using surface plasmon resonance (SPR). Secondly, their enhanced properties for biosensing were investigated. This was done by SPR using several SAMs in combination with various biological receptors and assays for a biological relevant model system: the detection of the cancer marker

Magnetic Resonance Materials in Physics, Biology and Medicine Editor-in-Chief: David Norris MAGMA is the Official Journal of the European Society for MagneticResonance in Medicine and Biology (ESMRMB). Join ESMRMB now and get free access to MAGMA! MAGMA is a multidisciplinary international journal devoted to the publication of articles on all aspects of magnetic resonance techniques and their applications in medicine and biology. The subject areas covered by MAGMA include advances in materials, hardware and software in magnetic resonance technology; new developments and results in research and practical applications of magnetic resonance imaging and spectroscopy related to biology and medicine; study of animal models and intact cells and reports of clinical trials on humans and clinical validation of magnetic resonance protocols ...

TY - JOUR. T1 - Developing role of magnetic resonance imaging in Crohns disease. AU - Lin, Michael F.. AU - Narra, Vamsidhar. PY - 2008/3/1. Y1 - 2008/3/1. N2 - PURPOSE OF REVIEW: There is growing concern among the medical community that diagnostic radiation adds to the already increased risk of developing lymphoma that may be inherent in, or related to the treatment of, inflammatory bowel disease. This article describes recent progress in magnetic resonance enterography techniques, and examines the role of MRI in the evaluation of Crohns disease. RECENT FINDINGS: Recent advancements in magnetic resonance technology and imaging protocol have made MRI of the small bowel feasible. With improved coils, breath-hold sequences and faster acquisition techniques, MRI capably depicts disease location, extent, and complications. Most of the current literature recognizes MRI as an excellent tool in characterizing transmural and extraluminal changes of Crohns disease. SUMMARY: The lack of ionizing ...

The aim of this work is the development of a cost effective, rapid, portable analysis system for the measurement of bioavailable toxic metal or organic contaminants, which may be present at natural attenuation landfill sites requiring remediation. The proposed system is intended to be used as a tool in the monitoring or characterisation of a site by discrete measurement. A portable system will be developed, comprising a miniaturised extraction and separation device employing capillary electrophoresis, detectors for specific analyses (metals or organics) by biosensors or immunosensors and dedicated instrumentation to process the signals generated. Development of extraction procedures for the analysis of plant material will also be part of the project, to be used when phytoremediation is employed as part of the site remediation process. Biosensors to detect toxic metal will use bioengineered proteins to interact with bioavailable metals on an electrode surface. The conformational change in the

Posted January 10th, 2012.. Novas new StatStrip™ Glucose Monitor has received clearance from the U.S. Food and Drug Administration for use in neonatal testing. Severe hematocrit abnormalities are routinely found in neonates. Other glucose monitors may report an error if the hematocrit value is abnormal, StatStrip is the only glucose monitor that measures hematocrit on the strip, automatically correcting glucose values for abnormal hematocrit values. In addition, its small 1.2 microliter sample volume is attractive for the NICU environment where blood conservation is essential.. Introduced in May 2006, StatStrip is rapidly becoming the gold standard in hospital glucose testing. Incorporating a patented new Multi-Well™ strip technology, StatStrip elevates bedside glucose monitoring to a level of speed, accuracy, quality, and patient safety on a par with central laboratory testing. In addition to hematocrit, StatStrip measures and corrects interferences from acetaminophen (Tylenol), uric acid, ...

Boyce Thompson Insitute scientist Maria Harrison will collaborate with Wayne Versaw of Texas A&M University to create a new imaging system to visualize and measure the movement of phosphate from soil fungi into plant root cells in a $1.2 million DOE-funded project.

Page contains details about SPR-based fiber optic ammonia sensor probe . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com

We present two strategies for microspotting 10 × 12 arrays of double-stranded DNAs (dsDNAs) onto a gold-coated glass slide for high-throughput studies of protein-DNA interactions by surface plasmon resonance (SPR) microscopy. Both methods use streptavidin (SA) as a linker layer between a biotin-containing mixed self-assembled monolayer (SAM) and biotinylated dsDNAs to produce arrays with high packing density. The primary mixed SAM is produced from biotin- and oligo(ethylene glycol)-terminated thiols bonded as thiolates onto the gold surface. In the first method, a robotic microspotter is used to deliver nanoliter droplets of dsDNA solution onto a uniform layer of this SA (~2 × 1012 SA/cm2). SPR microscopy shows a density of (5-6) × 1011 dsDNA/cm2 (0.2-0.3 dsDNA/SA) in the array elements. The second method uses instead a microspotted array of this SA linker layer, onto which the microspots of dsDNA are added with spatial registry. SPR microscopy before addition of the dsDNA shows a SA coverage ...

The GIA Smart Sleeve for the wrist is designed to help maintain wrist health by providing warmth, compression, and support where it is needed most. The power of GIAs proprietary Energy Resonance Technology (ERT) combined with the customized fit of the Far Infrared wrist sleeve supports the joint while leaving the thumb and fingers free for full range of motion. The firm support of the GIA wrist sleeve will help keep the wrist aligned and limit movements that would be painful or disruptive to the joint. Researchers have noted that Far-Infrared transfers energy purely in the form of heat, which can be perceived by the thermo-receptors in human skin as soothing warmth that can penetrate up to 1.5 inches beneath the skin. (Contains latex) - Available in: S / M / L / XL / XXL. ...

The GIA Smart Sleeve for the wrist is designed to help maintain wrist health by providing warmth, compression, and support where it is needed most. The power of GIAs proprietary Energy Resonance Technology (ERT) combined with the customized fit of the Far Infrared wrist sleeve supports the joint while leaving the thumb and fingers free for full range of motion. The firm support of the GIA wrist sleeve will help keep the wrist aligned and limit movements that would be painful or disruptive to the joint. Researchers have noted that Far-Infrared transfers energy purely in the form of heat, which can be perceived by the thermo-receptors in human skin as soothing warmth that can penetrate up to 1.5 inches beneath the skin. (Contains latex) - Available in: S / M / L / XL / XXL. ...