ARE THEY DRINKING? WELL FIND OUT.. Pinnacle Health Technologies (PHT) offers ethyl glucuronide (EtG) and ethyl sulfate (EtS) testing, known as the EtG/EtS Alcohol Testing solution. EtG is a direct metabolite of alcohol ethanol). Its presence in urine may be used to detect recent alcohol ingestion, even after ethanol is no longer measurable. The presence of EtG in urine is an indicator that alcohol was ingested. EtG is detectable in urine for up to 80 hours after the consumption of alcohol. EtG is only evident when alcohol is ingested and is not produced as a result of fermentation. In addition to EtG, recent scientific studies have identified ethyl sulfate (EtS) as a second specific metabolite or biomarker of ethanol. For this reason, PHT tests and reports EtS, in conjunction with EtG, to confirm recent ethanol ingestion or exposure. The detection of EtG and EtS offers greater sensitivity and accuracy for determination of recent alcohol ingestion, than by detection of either biomarker alone. ...

AMH Nationwide offers hair alcohol tests by ethyl glucuronide (ETG). Call us at ☎ (888) 493-2521 to find more information about a lab near you!

The direct alcohol marker ethyl glucuronide (EtG) is widely used for the assessment of alcohol consumption behavior and abstinence monitoring by hair analysis.

You can get a pretty good indicator of when the Ca(EtSO4)2 is neutralized when adding sodium carbonate solution does not produce a precipitate. Mix thoroughly and check the pH... I had the best luck with a pH of about 9... there was a lot of decomposition when evaporating the water with lower pH for some reason. I concentrated it on a hotplate, filtering any salts that precipitated out (calcium bicarbonate seems to be problematic here). Then I heated it on a hot water bath until the liquid hardened like rock candy when removed on a spatula and decanted onto a surface and allowed it to cool. Its somewhat easier to remove from aluminum foil than a glass plate. ...

Nokia sites use cookies to improve and personalize your experience and to display advertisements. The sites may also include cookies from third parties. By using this site, you consent to the use of cookies. Learn more ...

Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) are unique biomarkers of alcohol use. EtG and EtS analysis offers many advantages for abstinence monitoring including the detection window, stability in stored specimens, and specificity. EtG and EtS are both polar, making them difficult to retain via reversed-phase chromatography. Both compounds are also very sensitive to matrix interferences which can result in being unable to achieve low limits of detection and can also make quantitation impossible. In this study, a simple dilute and shoot method was developed and validated for the analysis of EtG and EtS in human urine by LC-MS/MS ...

2017. McDonell MG, Leickly E, McPherson S, Skalisky J, Srebnik S, Angelo F, Vilardaga R, Nepom JR, Roll JM, Ries RK. A randomized controlled trial of ethyl glucuronide-based contingency management for outpatients with co-occurring alcohol use disorders and serious mental illness. Am J Psychiatry. 2017; 174 (4): 370-377. PMCID: PMC5378651.. McDonell MG, Leickly E, McPherson S, Skalisky J, Hirchak K, Oluwoye O, Srebnik D, Roll JM, Richard RK. Pretreatment ethyl glucuronide levels predict response to a contingency management intervention for alcohol use disorders with serious mental illness. Am J Addict. 2017; 26(7):673-675. PMCID: PMC5610611.. Leickly E, Skalisky J, McPherson S, Orr MF, McDonell MG. High agreement between benchtop and point-of-care dipcard tests for ethyl glucuronide. Ther Drug Monit. 2017; 39(4):461-462. PMCID: PMC5513150.. ​. 2015. ​. McDonell MG, Skalisky J, Leickly E, McPherson S, Battalio S, Nepom JR, Srebnik D, Roll J, Ries RK. Using ethyl glucuronide in urine to detect ...

Zinc induces transition of a d(GA·CT)22 sequence to a novel DNA conformation, called *H-DNA. In this paper, the structural characteristics of this altered DNA conformation are determined. Formation of *H-DNA is induced at zinc concentrations higher than 70 μM (Zn/P = 2) and it is favoured by negative supercoiling and low ionic strength. Two different structural conformations of the d(GA·CT)22 sequence are observed upon increasing the zinc concentration. At low zinc concentration (Zn/P,15), half of the purine strand falls back upon itself giving rise to the formation of an RRY intramolecular triplex (H*-triplex). At higher zinc concentration, the complete pyrimidine strand is single-stranded and an RR hairpin is formed (*H-hairpin). Protection towards dimethylsulphate modification suggests that zinc binds to the N-7 group of guanine residues in the *H-hairpin with a higher affinity than in B-DNA. The dissociation constant of the *H-zinc complex is estimated to be in the range of 10-3 M to 10-4 ...

Two observations agree well with the binding site reported here. (i) The binding affinity determined for L6 compares well with that reported for the rRNA sequence 2640-2774 (Ka = 3 × 106 M−1; ref. 41), i.e., the rRNAL6-1 fragment. (ii) L6 changed the extent of base modifications with dimethylsulphate at three positions (41), and two of them are located within the binding site identified here (blue dots and triangles in Fig. 5B). In addition, Uchiumi et al. (41) showed that a mutation of the protected A2757 within the identified sequence abolishes L6 binding.. However, a crosslink between L6 and the loop of helix 89 around nucleotide 2475 (31, 42) is not covered by the L6 binding site reported here (see arrow in Fig. 5A). Therefore, we examined whether helix H89 is an L6 binding site that has escaped the selection process of the L6-SERF experiment. To this end, we synthesized helix H89 and tested it for binding to L6, with negative results (rRNAL6-3; Fig. 5B). This helix might be in the ...

ETG drug testing dip kits can be purchased here at Medical Distribution Group for a competitive price. Order online or give us a call today.

Acute (short-term) exposure of humans to the vapors of dimethyl sulfate may cause severe inflammation and necrosis of the eyes, mouth, and respiratory tract. Acute oral or inhalation exposure to dimethyl sulfate primarily damages the lungs but also injures the liver, kidneys, heart, and central nervous system (CNS), while dermal contact with dimethyl sulfate may produce severe blistering in humans. ...

Liquid urine controls are designed to monitor the performance with Immunoassay analyzers . Designed to match most testing cutoff levels on the market by leading Immunoassay companies such as Siemens, Roche, Danaher (Beckman) and Abbott. Our formulations are updated as market changes and new testing reagents become avai

Literature References: Manuf: by fermentation of starch, sugar, and other carbohydrates; from ethylene, acetylene, sulfite waste liquors, and synthesis gas (CO + H); by hydrolysis of ethyl sulfate, and oxidation of methane. Toxicity: G. S. Wiberg et al., Toxicol. Appl. Pharmacol. 16, 718 (1970). Embryotoxicity in mammals: N. A. Brown et al., Science 206, 573 (1979). Possible mechanism for actions of ethanol on the brain: G. Aston-Jones et al., Nature 296, 857 (1982). Ethanol-induced chromosomal abnormalities in mice: M. H. Kaufman, ibid. 302, 258 (1983). Disruption of reproductive function in female primates following alcohol self-administration: N. K. Mello et al., Science 221, 677 (1983). Review of metabolism and toxicity: C. S. Lieber in Reviews in Biochemical Toxicology vol. 5, E. Hodgson et al., Eds. (Elsevier, New York, 1983) pp 267-312; of pharmacology: L. Pohorecky, J. Brick, Pharmacol. Ther. 36, 335-427 (1988); of hepatotoxicity: C. S. Lieber, L. M. DeCarli, J. Hepatol. 12, 394-401 ...

초록. The overall folded (global) structure of mRNA may be critical to translation and turnover control mechanisms, but it has received little experimental attention. Presented here is a comparative analysis of the basic features of the global secondary structure of a synthetic mRNA and the same intracellular eukaryotic mRNA by dimethyl sulfate (DMS) structure probing. Synthetic MFA2 mRNA of Saccharomyces cerevisiae first was examined by using both enzymes and chemical reagents to determine single-stranded and hybridized regions; RNAs with and without a poly(A) tail were compared. A folding pattern was obtained with the aid of the MFOLD program package that identified the model that best satisfied the probing data. A long-range structural interaction involving the 5 and 3 untranslated regions and causing a juxtaposition of the ends of the RNA, was examined further by a useful technique involving oligo(dT)-cellulose chromatography and antisense oligonucleotides. DMS chemical probing of A and ...

Johnson-Davis KL, Slawson MH. Ethyl Glucuronide Positivity Rate in a Pain Management Population. J Anal Toxicol. 2015; 39 (9): 686-90.

This study reviews the literature evidence up until June 30, 2013, for the biologic and/or toxic effects of two prototype protein-bound uremic toxins: indoxyl sulfate and p-cresyl sulfate. In total, 27 studies from all over the world were retained, with the principal topics being endothelial and proximal tubular cell dysfunction, although insulin resistance, functional status of cardiac cells, leukocyte dysfunction, coagulation disturbances, and pharmacodynamics were also covered. A quality assessment identified 11 of 27 (41%) studies with a score of at least 4 points from a possible maximum of 5 points. The majority of the changes found affects mechanisms that are essential to the pathophysiology of the uremic syndrome and its subsequent deleterious impact on prognosis.. Endothelial dysfunction,26-30,33 smooth muscle cell lesions,46,51 coagulation disturbances,46 leukocyte activation,30,31 cross-talk of leukocytes and endothelium,30 cardiac fibrosis and hypertrophy,35,49 and insulin ...

Cardiovascular disease, the leading cause of mortality in hemodialysis patients, is not fully explained by traditional risk factors. To help define non-traditional risk factors, we determined the association of predialysis total p-cresol sulfate, indoxyl sulfate, phenylacetylglutamine, and hippurate with cardiac death, sudden cardiac death, and first cardiovascular event in the 1,273 participants of the HEMO Study. The results were adjusted for potential demographic, clinical, and laboratory confounders. The mean age of the patients was 58 years, 63% were Black and 42% were male. Overall, there was no association between the solutes and outcomes. However, in sub-group analyses, among patients with lower serum albumin (under 3.6 g/dl), a twofold higher p-cresol sulfate was significantly associated with a 12% higher risk of cardiac death (hazard ratio 1.12; 95% confidence interval, 0.98-1.27) and 22% higher risk of sudden cardiac death (1.22, 1.06-1.41). Similar trends were also noted with indoxyl ...

Indoxyl sulfate (IS) and p-cresyl sulfate (PCS) increased DNA methyltransferase (DNMT)1, 3a, and 3b expression in vitro. (a) Compared with HK2 cells without IS/

Background: Alcohol consumption is a frequently studied risk factor for chronic diseases, but many studies are hampered by self-report of alcohol consumption. The urinary metabolite ethyl glucuronide (EtG), reflecting alcohol consumption during the past 72 h, is a promising objective marker, but population data are lacking.. Objective: The objective of this study was to assess the reliability of EtG as a marker for habitual alcohol consumption compared with self-report and other biomarkers in the general population.. Methods: Among 6211 participants in the Prevention of Renal and Vascular End-Stage Disease (PREVEND) cohort, EtG concentrations were measured in 24-h urine samples. EtG was considered positive when concentrations were ,= 100 ng/mL. Habitual alcohol consumption was self-reported by questionnaire (categories: no/almost never, 1-4 units per month, 2-7 units per week, 1-3 units per day or ,= 4 units per day). Plasma HDL cholesterol concentration, erythrocyte mean corpuscular volume ...

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Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories. Uppsala, Sweden - Biotage® (STO: BIOT), a leading supplier of tools and technology for analytical and medicinal chemistry, introduced a new sample preparation application note for the extraction of ethyl glucuronide from urine by LC-MS.. EtG can be detected at very low levels and being a metabolite it is useful in distinguishing between ingested ethanol and that which is absorbed through the skin from ethanol containing products such as alcohol based hand washes. Rapid and reliable methods with robust extraction protocols are essential for analysis and quantitation of EtG from various matrices.. Advances in the manufacture of polymeric stationary phases and the recent release of EVOLUTE® AX, a mixed-mode ion exchange product, have allowed for the development of a more robust, cost-effective procedure. The protocol was developed on 96-well ...

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Ethyl Glucuronide, aka EtG is the best way to effectively monitor alcohol abstinence. No one would argue that the number one abused substance in America is alcohol. There are many ways to detect alcohol consumption, and breathalyzers are a wonderful tool in corrections for detecting someone under the influence. However, for those who encourage and monitor abstinence of alcohol, detecting its use after only a few hours had been extremely difficult. Negative breathalyzer readings, and traditional ethanol alcohol tests do not necessarily indicate abstinence.

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Relative molecular mass: 126.13 2.2. Physical and Chemical Properties The physical properties of dimethyl sulfate (DMS), which is a colourless, oily liquid, are summarized in Table 1. Commercial DMS may contain trace amounts of sulfuric acid. Most reports state that DMS is odourless, though some claim that it has a slight onion-like odour. The vapour pressure of DMS at 20 C would result in a saturated vapour concentration in air of 3720 mg/m3 (710 ppm) (Du Pont, 1981). It is miscible with many polar organic solvents and aromatic hydrocarbons, but is only sparingly soluble in aliphatic hydrocarbons and water. DMS is hydrolysed slowly in moist air or cold water, and more rapidly in warm water or acidic solutions. Initial hydrolytic products are monomethyl sulfate and methanol; complete conversion to sulfuric acid occurs more slowly (Robertson & Sugamori, 1966). DMS forms salts of monomethyl sulfate on hydrolysis in aqueous alkaline solutions (Du Pont, 1981). It reacts explosively with concentrated ...

Cytosolic Sulfotransferase 4A1/SULT4A1 Overexpression Lysate (Native). Tested Reactivity: Hu. Validated: WB. Backed by our 100% Guarantee.

About this substance This section provides an overview of the calculated volume at which the substance is manufactured or imported to the European Economic Area (EU28 + Iceland, Liechtenstein and Norway). Additionally, if available, information on the use of the substance and how consumers and workers are likely to be exposed to it can also be displayed here.. The use information is displayed per substance life cycle stage (consumer use, in articles, by professional workers (widespread uses), in formulation or re-packing, at industrial sites or in manufacturing). The information is aggregated from the data coming from REACH substance registrations provided by industry.. For a detailed overview on identified uses and environmental releases, please consult the registered substance factsheet.. Use descriptors are adapted from ECHA guidance to improve readability and may not correspond textually to descriptor codes described in Chapter R.12: Use Descriptor system of ECHA Guidance on information ...

Ethyl glucuronide (EtG) is a minor metabolite of alcohol that can be detected in hair. The objective of this study was to develop a validated method for the detection of EtG in hair samples. In addition, the research evaluated whether the level of ethyl glucuronide in hair can distinguish excessive v. social use of alcohol. Samples were collected from individuals who self-reported their daily or weekly consumption of alcohol. The hair samples were extracted and analyzed by GC-MS-MS. This study successfully developed a method to detect EtG in hair. EtG can be detected in concentrations from 1 pg/mg to 2 ng/mg. However, it was not possible to determine whether a correlation exists between a patients self-reported drinking history and the EtG concentrations found in their hair. Further research is needed to refine the extraction method ...

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These incredibly accurate saliva based drug tests are pefect for any job site or corporate setting. Less invasive that traditional urine tests, these tests are quick to read and dependable.

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Mouse Monoclonal Anti-Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody (4G1). Validated: WB, Flow, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.

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1. Spray, dip, or use a squirt bottle to apply R.A.Y. 2. Examination under a laser or Forensic Light Source at 450 nm to 550 nm. Use orange or red colored goggles. 3. Photograph results using orange or red barrier filter ...

said: You start to count your hours from the time your BAC is zero. You will produce ETG until your BAC is zero. If you can get at least 72 hours, you have a fairly good chance of passing. Hydrate early on after the drinking, then urinate one last time prior to your test to remove any ETG left in your bladder if there is any. You would be much safer to have at least 96 hours after a zero BAC ...

Different Types of Flotation Cells. Flotation is both a science and an art It brings together many complex variables Such basic factors as knowledge of mineral structure chemical reagents pH of mill water pulp density temperature technical skills of the operator the dependability of the flotation machine as well as a host of other factors which affect the flotation of each specific ore must be ...

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The S1 secondary alkylsulphohydrolase of the detergent-degrading micro-organism, Pseudomonas C12B, was separated from other alkylsulphohydrolases and purified to homogeneity. Under the experimental conditions used the enzyme completely hydrolysed d-octan-2-yl sulphate (d-1-methylheptyl sulphate), but showed no activity towards the corresponding l-isomer. Additional evidence has been obtained to indicate that it is probably optically stereospecific for d-secondary alkyl sulphate esters with the ester sulphate group at C-2 and with a chain length of at least seven carbon atoms. Enzyme activity towards racemic samples of heptan-2-yl sulphate (1-methylhexyl sulphate), octan-2-yl sulphate and decan-2-yl sulphate (1-methylnonyl sulphate) increased with increasing chain length. l-Octan-2-yl sulphate is a competitive inhibitor of the enzyme, as are certain primary alkyl sulphates and primary alkanesulphonates. Inhibition by each of the last two types of compounds is characteristic of the behaviour of an ...

Looking for online definition of Chemical reagent in the Medical Dictionary? Chemical reagent explanation free. What is Chemical reagent? Meaning of Chemical reagent medical term. What does Chemical reagent mean?

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It is a typical apply inside the cosmetics enterprise, especially referring to the foremost producers, to keep costs down by using solely primarily essentially the most cost environment friendly substances doable. That is the method that these corporations assure their products have the very best doable revenue margin. This comply with could also be helpful to them, but it is detrimental to their prospects. What is a EtG alcohol testing ? An EtG, or ethyl glucuronide test could also be preformed through various means; two particularly are urine alcohol testing and hair alcohol testing. An EtG check out detected through urine samples, or with scalp hair samples, test the presence of EtG within the type of a biomarker. EtG or Ethyl glucuronide is a metabolite only produced when alcohol is within the bloodstream. The extra alcohol an individual consumes, the higher quantity of EtG markers are current throughout the hair or urine sample. There are several variations between hair and urine ...

What are Annulment Varieties ? Whats a EtG alcohol testing ? An EtG, or ethyl glucuronide verify will be preformed by way of numerous means; two particularly are urine alcohol testing and hair alcohol testing. An EtG examine detected by way of urine samples, or with scalp hair samples, check the presence of EtG within the kind of a biomarker. EtG or Ethyl glucuronide is a metabolite only produced when alcohol is within the bloodstream. The extra alcohol a person consumes, the higher quantity of EtG markers are current in the hair or urine pattern. There are a variety of variations between hair and urine samples. For a very long time I have been involved about why at the moments career-minded women are smart about work but not love. I didnt have a full sufficient reply, and when something nags at me, I do analysis. So, for five years, I studied more than 1,200 girls from everywhere in the world. My findings led to 2 research-based, self-help books: Good Relationships, printed by New Horizon ...

It is a typical observe within the cosmetics commerce, especially in the case of the primary producers, to keep up costs down by using solely probably essentially the most value efficient substances possible. This is the way during which that these corporations guarantee their merchandise have the most effective doable profit margin. This apply could also be useful to them, nonetheless it is detrimental to their clients. Whats a EtG alcohol testing ? An EtG, or ethyl glucuronide test might be preformed by numerous means; two particularly are urine alcohol testing and hair alcohol testing. An EtG check detected by means of urine samples, or with scalp hair samples, take a look at the presence of EtG within the form of a biomarker. EtG or Ethyl glucuronide is a metabolite only produced when alcohol is in the bloodstream. The more alcohol a person consumes, the larger amount of EtG markers are present within the hair or urine pattern. There are a number of differences between hair and urine ...

Its a typical observe in the cosmetics trade, particularly within the case of the principle manufacturers, to maintain prices down by utilizing solely most likely essentially the most value efficient substances potential. This is the best way by which that these companies guarantee their merchandise have one of the best doable revenue margin. This apply may be useful to them, nonetheless its detrimental to their prospects. Whats a EtG alcohol testing ? An EtG, or ethyl glucuronide examine might be preformed by quite a few means; two specifically are urine alcohol testing and hair alcohol testing. An EtG test detected by the use of urine samples, or with scalp hair samples, test the presence of EtG within the type of a biomarker. EtG or Ethyl glucuronide is a metabolite solely produced when alcohol is within the bloodstream. The extra alcohol a person consumes, the greater amount of EtG markers are present in the hair or urine pattern. There are a number of differences between hair and urine ...

It is a typical observe within the cosmetics trade, particularly within the case of the main producers, to take care of costs down by using solely in all probability the most worth efficient ingredients potential. That is the way in which by which that these corporations guarantee their merchandise have the perfect doable profit margin. This follow could also be helpful to them, nonetheless its detrimental to their customers. Whats a EtG alcohol testing ? An EtG, or ethyl glucuronide test will likely be preformed by quite a few means; two specifically are urine alcohol testing and hair alcohol testing. An EtG verify detected by way of urine samples, or with scalp hair samples, take a look at the presence of EtG within the form of a biomarker. EtG or Ethyl glucuronide is a metabolite solely produced when alcohol is within the bloodstream. The extra alcohol an individual consumes, the larger quantity of EtG markers are current in the hair or urine pattern. There are a number of differences ...

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Looking for diethyl sulfate? Find out information about diethyl sulfate. 2SO4 A colorless oil with a peppermint odor, and boiling at 208°C; used as an intermediate in organic synthesis. Also known as ethyl sulfate Explanation of diethyl sulfate

Cardiovascular disease is the number one killer of patients with chronic kidney disease (CKD), and insulin resistance - a lowered level of response to insulin circulating in the blood - is an important cardiovascular risk factor in these patients. Its not clear why patients with CKD often develop insulin resistance, but the retention of compounds that are normally removed from the blood and excreted in the urine may play a role. One such compound is p-cresyl sulfate (PCS), a toxin that is produced by gut bacteria. PCS is retained in CKD patients, and it is poorly removed by most dialysis techniques.. Read more. ...

Figure 1. Time-averaged plasma solute levels in patients undergoing conventional thrice-weekly hemodialysis. Uremic solutes that are relatively large compared to urea are poorly cleared by even high flux dialyzers. The increase in the level of p-cresol sulfate would be of even greater magnitude if free rather than total plasma solutes levels were compared. ...

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1. Treat item with cyanoacrylate. 2. Submerge the item in the reagent or use a squirt bottle to apply. 3. Allow item to air dry. 4. Distilled water rinse. Again allow item to air dry. 5. Examination under a laser or Forensic Light Source at 470 nm to 550 nm. Absorption Max is 510 nm. Use orange colored goggles. 6. Photograph results using an orange or bandpass 550(BP35) barrier filter ...

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3-Indoxyl sulfate is a commonly used chromogenic substrate for arylsulfatase, yielding a blue precipitate upon cleavage. Free Shipping within the Continental USA ...

Curcumin is characterized with extremely low solubility in water (11ng/ml) and significant presystemic biotrans formation, mainly via glucuronide and sulfate conjugation.The maximum oral dose of 8 gm/day of curcumin does not produce any toxic effects

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In the event of equipment malfunction, shortage of chemical reagents, or staffing limitations due to holidays, the DNA Synthesis division has established contracts with a third-party vendor to fulfill our customers oligonucleotide orders (including free shipment to our facility). In these cases, the end user will not see any increases in the cores standard pricing structures. Please note that appropriate adjustments of the synthesis scale ordered may take place to conform to the synthesis service options of our preferred third-party vendor.. ...

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Acetic acid is one of the simplest carboxylic acids. It is an important chemical reagent and is used in many staining procedures in a dilute form.

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We recently had the opportunity to speak with Dr. Kamisha Johnson-Davis, PhD regarding EtG and EtS testing accuracy using urine drug tests.

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Synthetic sulfates are the ones you should be worried about. Learn the facts about this ingredient and what you should do about it.