Project 1: Bipolar Tetraether Lipid Membranes: Physical Properties, and Technological Applications. The long-term goals of this research are to understand how the Archaea live in extreme environments and to use the Archaeal bipolar tetraether lipids for technological applications. The Archaea are curious and remarkable organisms; and, their lipids are structurally distinctly different from their bacterial and eukaryotic counterparts. The native habitat of the thermoacidophilic archaeon Sulfolobus acidocaldarius, which is the focus of this research, is hot (65-80 oC) and acidic (pH 2-3) sulfur springs. The plasma membrane of S. acidocaldarius not only serves as a barrier between the low pH extracellular environment and the neutral pH intracellular compartment (pH 6.5), but also performs proton pumping and other cellular activities at high temperatures. The ability of the plasma membrane to achieve these goals in extreme environments is not clearly understood, although there is evidence suggesting ...
Sulfolobus acidocaldarius ATCC ® 33909™ Designation: DSM 639 TypeStrain=True Application: Produces citrate synthase Produces endodeoxyribonuclease HaeIII SuaI restriction endonuclease, restriction endonuclease SuaI
The crenarchaeon S. acidocaldarius utilizes different carbohydrates, including pentoses such as d-xylose and l-arabinose (14, 15, 38). However, so far no transport system for d-xylose could be identified, and whether pentose assimilation strictly relies on both the aldolase-dependent and the aldolase-independent pathways is not yet fully understood. In this study, we compared growth on complex, proteinaceous media in the absence or presence of d-xylose. RNA sequencing of S. acidocaldarius MW001 revealed only five highly upregulated transcripts in response to d-xylose. Three of them encode an unknown and uncharacterized ABC transport system, and two represent enzymes of the previously reported aldolase-independent pathway.. The identified ABC transport system is not conserved within the Sulfolobales or found in any other archaea. The SBP Saci_2122 revealed a closer relationship to bacterial SBPs. Additionally, the NBD-encoding gene saci_2120 was previously not annotated. Therefore, the ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Sulfolobus Sac7 protein: RN refers to Sac7d; MW 7 kDa; small basic DNA-binding protein from Sulfolobus acidocaldarius; amino acid sequence given in first source
Thermoacidophilic archaeon dehydrogenase. Computer model showing the structure of 3-isopropylmalate dehydrogenase from Sulfolobus acidocaldarius. - Stock Image C035/6196
General Information: Sulfolobus acidocaldarius DSM 639 was isolated from and acidic hot spring in Yellowstone National Park. Extreme thermoacidophilic sulfur-oxidizing archaeon. This organsim is an extreme thermoacidophilic, sulfur-oxidizing archaeon commonly found in hot springs growing at very high temperatures. This obligate aerobe is immotile and grows at a temperature of 55-85 degrees C with optimal growth at 70-75 degrees C. The pH for growth is 1-6 with an optimum pH 2-3. ...
Until now little was known about the proteins that control cell division in the Archaea. With the use of immunofluorescence the researchers determined the location of these proteins in the cell and in doing so discovered that three proteins play a crucial role in the cell division of Sulfolobus acidocaldarius. Once the whole chromosome has been replicated, these three proteins form a band-like structure over the cell equator. One chromosome is then found on each side of this band. The band then squeezes the cell into two so that two new daughter cells are formed. At first it looks like mitosis, as discussed in many a biology lesson. However, mitosis is the process whereby the chromosomes are distributed between the two daughter cells. Cell division is the process whereby the two daughter cells are separated ...
Affitins (commercial name Nanofitins) are artificial proteins with the ability to selectively bind antigens. They are structurally derived from the DNA binding protein Sac7d, found in Sulfolobus acidocaldarius, a microorganism belonging to the archaeal domain. By randomizing the amino acids on the binding surface of Sac7d and subjecting the resulting protein library to rounds of ribosome display, the affinity can be directed towards various targets, such as peptides, proteins, viruses,[citation needed] and bacteria. Affitins are antibody mimetics and are being developed as an alternative to antibodies as tools in biotechnology. They have also been used as specific inhibitors for various enzymes.[citation needed] Affitins consist of 66 amino acids and have a molecular mass of about 7 kDa, small compared to antibodies with some 130-150 kDa. Being obtained from a thermophile organism, they are unusually heat resistant proteins. Unlike antibodies, affitins are produced in vitro, and therefore can be ...
Habitat heterogeneity is a driving factor for speciation and ecosystem functioning and is well studied in macro-ecology. Yet our understanding of microbial adaptations, and governing processes is incomplete. The here presented thesis aims at giving us a better understanding of patterns in micro-heterogeneity, and microbial adaptations to such heterogeneity with particular focus on surface-dominated, aquatic habitats. The most prominent microbial adaptation to surface associated mode of life is biofilm formation. Biofilms rely heavily on type IV pili. These pili systems are well studied in Bacteria, but largely unknown in Archaea. Therefore, the first part of this thesis focuses on resolving genetic and structural feature of the type IV like aap-pilus of the thermo-acidophilic Sulfolobus acidocaldarius. We found the aap-pilus to be indispensible for biofilm formation, and to be unparalleled in variability of its quaternary structure and cross regulation with other filaments. The second part of ...
A2 hmmalign DR ECOCYC; EG10383; glnA; DR EXPERIMENTAL; EGAD,20324,EC3870; DR EXPERIMENTAL; EGAD,8994,8799; RN [1] RM 99081206 RT The glutamine synthetase from the hyperthermoacidophilic crenarcheon Sulfolobus acidocaldarius: isolation, characterization and sequencing of the gene. RA Yin Z, Purschke WG, Schafer G, Schmidt CL RL Biol Chem 1998 Nov;379(11):1349-54 RN [2] RM 95214531 RT Glutamine synthetase gene evolution in bacteria. RA Pesole G, Gissi C, Lanave C, Saccone C RL Mol Biol Evol 1995 Mar;12(2):189-97 ...
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43] Kouril T, Wieloch P, Reimann J, Wagner M, Zaparty M, Albers SV, Schomburg D, Ruoff P, Siebers B (2012). Unraveling the function of the two Entner-Doudoroff branches in the thermoacidophilic Crenarchaeon Sulfolobus solfataricus P2. FEBS J. 2012 Dec 24. doi: 10.1111/febs.12106. PMID:23279921. [42] Peetsch A, Greulich C, Braun D, Stroetges C, Rehage H, Siebers B, Köller M, Epple M (2012). Silver-doped calcium phosphate nanoparticles: Synthesis, characterization, and toxic effects toward mammalian and prokaryotic cells. Colloids Surf B Biointerfaces. 2013 Feb 1;102:724-9. doi: 10.1016/j.colsurfb.2012.09.040. Epub 2012 Oct 6. PMID:23107950. [41] Ulas T, Riemer SA, Zaparty M, Siebers B, Schomburg D (2012). Genome-scale reconstruction and analysis of the metabolic network in the hyperthermophilic archaeon Sulfolobus solfataricus. PLoS One. 2012;7(8):e43401. doi: 10.1371/journal.pone.0043401. Epub 2012 Aug 31. PMID:22952675. [40] Esser D, Pham TK, Reimann J, Albers SV, Siebers B, Wright PC (2012). ...
Fingerprint Dive into the research topics of Metabolism of pentose sugars in the hyperthermophilic archaea ,em,sulfolobus solfataricus,/em, and ,em,sulfolobus acidocaldarius,/em,. Together they form a unique fingerprint. ...
TY - JOUR. T1 - The hyperthermophilic cystathionine c-synthase from the aerobic crenarchaeon Sulfolobus tokodaii. T2 - Expression, purification, crystallization and structural insights. AU - Sato, Dan. AU - Shiba, Tomoo. AU - Mizuno, Sae. AU - Kawamura, Ayaka. AU - Hanada, Shoko. AU - Yamada, Tetsuya. AU - Shinozaki, Mai. AU - Yanagitani, Masahiko. AU - Tamura, Takashi. AU - Inagaki, Kenji. AU - Harada, Shigeharu. PY - 2017. Y1 - 2017. N2 - Cystathionine γ-synthase (CGS; EC 2.5.1.48), a pyridoxal 5′-phosphate (PLP)-dependent enzyme, catalyzes the formation of cystathionine from an l-homoserine derivative and l-cysteine in the first step of the transsulfuration pathway. Recombinant CGS from the thermoacidophilic archaeon Sulfolobus tokodaii (StCGS) was overexpressed in Escherichia coli and purified to homogeneity by heat treatment followed by hydroxyapatite and gel-filtration column chromatography. The purified enzyme shows higher enzymatic activity at 353 K under basic pH conditions compared ...
DNA mismatch repair (MMR) is a highly conserved biological pathway that plays a key role in maintaining genomic stability. MMR corrects DNA mismatches generated during DNA replication, thereby preventing mutations from becoming permanent in dividing cells. MMR also suppresses homologous recombination and was recently shown to play a role in DNA damage signaling. Defects in MMR are associated with genome-wide instability, predisposition to certain types of cancer including HNPCC, resistance to certain chemotherapeutic agents, and abnormalities in meiosis and sterility in mammalian systems. The Escherichia coli MMR pathway has been extensively studied and is well characterized. In E. coli, the mismatch-activated MutS-MutL-ATP complex licenses MutH to incise the nearest unmethylated GATC sequence. UvrD and an exonuclease generate a gap. This gap is filled by pol III and DNA ligase. The GATC sites are then methylated by Dam. Several human MMR proteins have been identified based on their homology to ...
Most acidophile organisms have evolved extremely efficient mechanisms to pump protons out of the intracellular space in order to keep the cytoplasm at or near neutral pH. Therefore, intracellular proteins do not need to develop acid stability through evolution. However, other acidophiles, such as Acetobacter aceti, have an acidified cytoplasm which forces nearly all proteins in the genome to evolve acid stability.[7] For this reason, Acetobacter aceti has become a valuable resource for understanding the mechanisms by which proteins can attain acid stability. Studies of proteins adapted to low pH have revealed a few general mechanisms by which proteins can achieve acid stability. In most acid stable proteins (such as pepsin and the soxF protein from Sulfolobus acidocaldarius), there is an overabundance of acidic residues which minimizes low pH destabilization induced by a buildup of positive charge. Other mechanisms include minimization of solvent accessibility of acidic residues or binding of ...
A new type of metal centre was detected in the membranes of the thermoacidophilic archaeon Sulfolobus metallicus. This centre has an S = 1/2 ground state in the oxidised form, yielding an axial EPR signal with g values at 2.035 (g(parallel)) and 1.97
Only three sets of proteins of the Alba superfamily are experimentally characterized: Sulfolobus Alba, Rpp20 proteins from yeast and vertebrates and the Rpp25 protein from vertebrates. The former has been shown to be the major chromosomal DNA-binding protein in the crenarchaeon Sulfolobus. However, even in Sulfolobus, Alba coats DNA densely but does not elicit significant compaction, suggesting that it is not sufficient for chromatin organization and requires functional partners, such as Sul7d [6]. Furthermore, at least in eukaryotes, even if Alba is a chromosomal protein it does appear to be as abundant as some of the other proteins such as HMG I/Y, HMG1 or related proteins [14]. Experimental studies on euryarchaeal chromatin have, so far, not yielded much evidence for a major chromosomal role for Alba [5, 8, 27]. Hence, it is conceivable that Alba was specifically recruited for a major chromosomal function only in a particular lineage of crenarchaea.. Rpp20/Pop7 from S. cerevisiae and humans ...
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TY - JOUR. T1 - A broadly applicable gene knockout system for the thermoacidophilic archaeon Sulfolobus islandicus based on simvastatin selection. AU - Zhang, Changyi. AU - Whitaker, Rachel J.. PY - 2012/6. Y1 - 2012/6. N2 - Sulfolobus species have been developed as excellent model organisms to address fundamental questions of archaeal biology. Interesting patterns of natural variation among Sulfolobus islandicus strains have been identified through genome sequencing. Experimentally testing hypotheses about the biological causes and consequences of this natural variation requires genetic tools that apply to a diversity of strains. Previously, a genetic transformation system for S. islandicus was reported, in which overexpression of the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene on the shuttle vector pSSR allowed the selection of transformants resistant to high concentrations of the thermostable antibiotic simvastatin. Here, we developed a novel gene knockout system based on ...
TY - JOUR. T1 - Metabolism of pentose sugars in the hyperthermophilic archaea sulfolobus solfataricus and sulfolobus acidocaldarius. AU - Nunn, C E M. AU - Johnsen, U. AU - Schonheit, P. AU - Fuhrer, T. AU - Sauer, U. AU - Hough, David W. AU - Danson, Michael J. PY - 2010/10/29. Y1 - 2010/10/29. N2 - We have previously shown that the hyperthermophilic archaeon, Sulfolobus solfataricus, catabolizes D-glucose and D-galactose to pyruvate and glyceraldehyde via a non-phosphorylative version of the Entner-Doudoroff pathway. At each step, one enzyme is active with both C6 epimers, leading to a metabolically promiscuous pathway. On further investigation, the catalytic promiscuity of the first enzyme in this pathway, glucose dehydrogenase, has been shown to extend to the C5 sugars, D-xylose and L-arabinose. In the current paper we establish that this promiscuity for C6 and C5 metabolites is also exhibited by the third enzyme in the pathway, 2-keto-3-deoxygluconate aldolase, but that the second step ...
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.
Comparative 16S rRNA (rDNA) sequence analyses performed on the thermophilic Bacillus species Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus revealed that these organisms are sufficiently different from the traditional Bacillus species to warrant reclassification in a new genus, Alicyclobacillus gen. nov. An analysis of 16S rRNA sequences established that these three thermoacidophiles cluster in a group that differs markedly from both the obligately thermophilic organism Bacillus stearother-mophilus and the facultatively thermophilic organism Bacillus coagulans, as well as many other common mesophilic and thermophilic Bacillus species. The thermoacidophilic Bacillus species B. acidocaldarius, B. acidoterrestris, and B. cycloheptanicus also are unique in that they possess ω-alicylic fatty acid as the major natural membranous lipid component, which is a rare phenotype that has not been found in any other Bacillus species characterized to date. This phenotype, along with
Investigations were performed on the structural features responsible for kinetic thermal stability of a thermostable carboxypeptidase from the thermoacidophilic archaebacterium Sulfolobus solfataricus which had been purified previously and identified as a zinc metalloprotease [Colombo, DAuria, Fusi, Zecca, Raia and Tortora (1992) Eur. J. Biochem. 206, 349-357]. Removal of Zn2+ by dialysis led to reversible activity loss, which was promptly restored by addition of 80 microM ZnCl2 to the assay mixture. For the first-order irreversible thermal inactivation the metal-depleted enzyme showed an activation energy value of 205.6 kJ.mol-1, which is considerably lower than that of the holoenzyme (494.4 kJ.mol-1). The values of activation free energies, enthalpies and entropies also dropped with metal removal. Thermal inactivation of the apoenzyme was very quick at 80 degrees C, whereas the holoenzyme was stable at the same temperature. These findings suggest a major stabilizing role for the bivalent ...
General Information: Isolation: Mariner hydrothermal vent field in the South Pacific Ocean; Temp: Thermophile; Temp: 70C; Habitat: Deep sea, Hydrothermal vent, Marine. Acidophilic thermophile. Aciduliprofundum boonei is an obligate thermoacidophile growing at pH between 3.3 - 5.8 with an optimum temperature of 70 degrees C. This organism is a chemoorganotroph, using iron and sulfur as electron donors. ...
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DNA supercoiling plays essential role in maintaining proper chromosome structure, as well as the equilibrium between genome dynamics and stability under specific physicochemical and physiological conditions. In mesophilic organisms, DNA is negatively supercoiled and, until recently, positive supercoiling was considered a peculiar mark of (hyper)thermophilic archaea needed to survive high temperatures. However, several lines of evidence suggest that negative and positive supercoiling might coexist in both (hyper)thermophilic and mesophilic organisms, raising the possibility that positive supercoiling might serve as a regulator of various cellular events, such as chromosome condensation, gene expression, mitosis, sister chromatid cohesion, centromere identity and telomere homoeostasis.. ...
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts extremozymes for utilization in White Biotechnology. Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012 ...
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts extremozymes for utilization in White Biotechnology. Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012 ...
GF ID SSV1_ORF_D-335 #=GF AC PF07935.10 #=GF DE ORF D-335-like protein #=GF AU Fenech M #=GF SE Pfam-B_4933 (release 14.0) #=GF GA 21.60 21.60; #=GF TC 22.10 44.60; #=GF NC 20.70 21.20; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq #=GF TP Family #=GF RN [1] #=GF RM 1926776 #=GF RT Complete nucleotide sequence of the virus SSV1 of the #=GF RT archaebacterium Sulfolobus shibatae. #=GF RA Palm P, Schleper C, Grampp B, Yeats S, McWilliam P, Reiter WD, #=GF RA Zillig W; #=GF RL Virology 1991;185:242-250. #=GF DR INTERPRO; IPR012922; #=GF CC The sequences featured in this family are similar to a probable #=GF CC integrase (Swiss:P20214) expressed by the SSV1 virus of the #=GF CC archaebacterium Sulfolobus shibatae. This protein may be #=GF CC necessary for the integration of the virus into the host genome #=GF CC by a process of site-specific recombination [1]. #=GF SQ 17 #=GS Q980Y3_SULSO/40-112 AC Q980Y3.1 #=GS W7KM36_9CREN/1-47 AC W7KM36.1 #=GS ...
Turriviridae is a family of viruses; it contains only one genus, Alphaturrivirus. The archaea Sulfolobus solfataricus serve as natural hosts. There are currently only two species in the genus Alphaturrivirus. Viruses in Turriviridae have icosahedral geometries, and T=31 symmetry. The diameter is around 74 nm. Genomes are linear. Viral replication is cytoplasmic. Entry into the host cell is achieved by adsorption into the host cell. DNA-templated transcription is the method of transcription. Sulfolobus solfataricus serves as the natural host. Transmission routes are passive diffusion. Viral Zone. ExPASy. Retrieved 13 August 2015. ICTV. Virus Taxonomy: 2014 Release. Retrieved 13 August 2015. Viralzone: Turriviridae ...
Electrical Chip (E-Chip) system offers a fast, sensitive and cost-effective way to detect analyte. To improve its application of nucleic acids detection, a suitable enzyme reporter is expected. Esterase 2 (EST2) from Alicyclobacillus acidocaldarius was introduced and mutated to have an accessible cysteine residue at 118th codon. This esterase was purified by a single-step affinity chromatography with trifluoromethyl ketone as a ligand and covalently conjugated to a 5-amino modified oligodeoxynucleotide. The purified conjugate served as a reporter enzyme for electrochemical detection of nucleic acids. Being an optimal substrate, p-aminophenylbutyrate exerts maximal signal response to EST2 in E-Chip, as determined by comparison of p-aminophenyl esters with acyl chain length from two to eight carbons. An assay of 15 pM of soluble esterase 2 in 1 ml was obtained exploiting p-aminophenylbutyrate. E-Chip detection of nucleic acids requires three essential steps: immobilization of thiol-modified ...
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Display systems to present biologically active molecules on the surface of microorganisms have become an increasingly used strategy to address biotechnological issues [1, 2]. For biomedical applications surface display systems have been mostly used for the identification of neutralizing epitopes, the development of whole cell diagnostic tools, or vaccine delivery [3, 4]. More recent is a strategy to engineer bacterial endospores (spores) to display heterologous proteins on their surface [5]. Endospore-forming bacteria are Gram-positive microorganisms belonging to different genera and including more than 1,000 species [6]. The common feature of these organisms is the ability to form a quiescent cellular type (the spore) in response to harsh environments. The spore can survive in this dormant state for long periods, resisting to a vast range of stresses such as high temperature, dehydration, absence of nutrients, presence of toxic chemicals. When the environmental conditions ameliorate, the spore ...
Author: Laso-Pérez, R. et al.; Genre: Journal Article; Published in Print: 2016-11-17; Open Access; Keywords: Archaeal physiology; Metabolomics; Environmental microbiology|br/|; Title: Thermophilic archaea activate butane via alkyl-coenzyme M formation.
Chimeric models claim that two prokaryotic cells existed initially - an archaeon and a bacterium. These cells underwent a merging process, either by a physical fusion or by endosymbiosis, thereby leading to the formation of a eukaryotic cell. Within these chimeric models, some studies further claim that mitochondria originated from a bacterial ancestor while others emphasize the role of endosymbiotic processes behind the origin of mitochondria. Based on the process of mutualistic symbiosis, the hypotheses can be categorized as - the serial endosymbiotic theory (SET),[122][123][124] the hydrogen hypothesis (mostly a process of symbiosis where hydrogen transfer takes place among different species),[117] and the syntrophy hypothesis.[125][126]. According to serial endosymbiotic theory (championed by Lynn Margulis), a union between a motile anaerobic bacterium (like Spirochaeta) and a thermoacidophilic crenarchaeon (like Thermoplasma which is sulfidogenic in nature) gave rise to the present day ...
GDNT - Glycerol Dialkyl Nonitol Tetraether. Looking for abbreviations of GDNT? It is Glycerol Dialkyl Nonitol Tetraether. Glycerol Dialkyl Nonitol Tetraether listed as GDNT
Lübben, M. , Güldenhaupt, J. , Zoltner, M. , Deigweiher, K. , Haebel, P. , Urbanke, C. and Scheidig, A. J. (2007): Sulfate Acts as Phosphate Analog on the Monomeric Catalytic Fragment of the CPx-ATPase CopB from Sulfolobus solfataricus , Journal of Molecular Biology ...
PDCD5蛋白質是個最早被發現在人類體內細胞用來上調節的細胞凋亡蛋白。PDCD5蛋白質可從細胞質向細胞核迅速的通過並與Tip60蛋白結合於DNA造成乙醯化,進而傳遞細胞凋亡訊息,其重要性為調節第五型賴氨酸乙酰轉化酶抑制其蛋白酶體相關的降解(參與轉錄、DNA損傷反應和細胞週期控制蛋白)。我們從KEGG數據分析庫確定了SSO0352基因為PDCD5蛋白在古生菌Sulfolobus solfataricus的同源基因,由於目前在古生菌Sulfolobus solfataricus的功能仍然還是未知,為了研究是否有相似的作用及結構。我們表現在質體pET-21a上和找到適合生產PDCD5蛋白質的方法,並表現於大腸桿菌(RIL)菌株,找到其純化條件。利用陽離子交換樹脂和膠體交換法純化出蛋白並使用X-ray繞射與單重原子異常散射(SAD)得知PDCD5蛋白的結構。Sso-PDCD5晶體在X-ray的繞射數據為Space group=C2, a = 100.34 Å, b = 39.71 Å, c = 77.57 ...
Viruses infecting the Archaea exhibit a tremendous amount of morphological and genetic diversity. This is especially true for crenarchaeal viruses from the family Fuselloviridae, which possess spindle-shaped capsids and genomes that harbor a great number of uncharacterized genes. The functions of these unidentified gene products are of interest as they have the potential to provide valuable insights into the fusellovirus infection cycle and archaeal viruses in general. In an effort to better characterize the genetic requirements of the Fuselloviridae, we have performed genetic and biochemical experiments using the best studied fusellovirus, Sulfolobus spindle-shaped virus 1 (SSV1). A comprehensive genetic analysis of SSV1 was conducted using long inverse PCR and transposon mutagenesis. The results of this work illustrate that SSV1 is highly tolerant of mutagenesis. A robust protocol for the purification of recombinant VP2 protein from E. coli was developed and should be useful for future studies aimed
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Identification of plasmid pTC1 from Sulfolobus tengchongensis RT8-4. (a) Restriction digestion of the total DNA from S. tengchongensis RT8-4 containing pTC1. Th
Ismail, Hesham M; Krishnamoorthy, Navaneethakrishnan; Al-Dewik, Nader; Zayed, Hatem; Mohamed, Nura A; Giacomo, Valeria Di; Gupta, Sapna; Häberle, Johannes; Thöny, Beat; Blom, Henk J; Kruger, Waren D; Ben-Omran, Tawfeg; Nasrallah, Gheyath K ...
Within a few rounds, it became clear who was going to win the game. In fact, by the episodes penultimate round, Nura had swept the board and secured $13,970 in the bank. In contrast, Troy had bagged just $4,300, and poor Steve hadnt managed to win anything at all.. ...
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