TY - JOUR. T1 - A broadly applicable gene knockout system for the thermoacidophilic archaeon Sulfolobus islandicus based on simvastatin selection. AU - Zhang, Changyi. AU - Whitaker, Rachel J.. PY - 2012/6. Y1 - 2012/6. N2 - Sulfolobus species have been developed as excellent model organisms to address fundamental questions of archaeal biology. Interesting patterns of natural variation among Sulfolobus islandicus strains have been identified through genome sequencing. Experimentally testing hypotheses about the biological causes and consequences of this natural variation requires genetic tools that apply to a diversity of strains. Previously, a genetic transformation system for S. islandicus was reported, in which overexpression of the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene on the shuttle vector pSSR allowed the selection of transformants resistant to high concentrations of the thermostable antibiotic simvastatin. Here, we developed a novel gene knockout system based on ...
TY - JOUR. T1 - The hyperthermophilic cystathionine c-synthase from the aerobic crenarchaeon Sulfolobus tokodaii. T2 - Expression, purification, crystallization and structural insights. AU - Sato, Dan. AU - Shiba, Tomoo. AU - Mizuno, Sae. AU - Kawamura, Ayaka. AU - Hanada, Shoko. AU - Yamada, Tetsuya. AU - Shinozaki, Mai. AU - Yanagitani, Masahiko. AU - Tamura, Takashi. AU - Inagaki, Kenji. AU - Harada, Shigeharu. PY - 2017. Y1 - 2017. N2 - Cystathionine γ-synthase (CGS; EC 2.5.1.48), a pyridoxal 5′-phosphate (PLP)-dependent enzyme, catalyzes the formation of cystathionine from an l-homoserine derivative and l-cysteine in the first step of the transsulfuration pathway. Recombinant CGS from the thermoacidophilic archaeon Sulfolobus tokodaii (StCGS) was overexpressed in Escherichia coli and purified to homogeneity by heat treatment followed by hydroxyapatite and gel-filtration column chromatography. The purified enzyme shows higher enzymatic activity at 353 K under basic pH conditions compared ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
A new type of metal centre was detected in the membranes of the thermoacidophilic archaeon Sulfolobus metallicus. This centre has an S = 1/2 ground state in the oxidised form, yielding an axial EPR signal with g values at 2.035 (g(parallel)) and 1.97
GF ID SSV1_ORF_D-335 #=GF AC PF07935.10 #=GF DE ORF D-335-like protein #=GF AU Fenech M #=GF SE Pfam-B_4933 (release 14.0) #=GF GA 21.60 21.60; #=GF TC 22.10 44.60; #=GF NC 20.70 21.20; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq #=GF TP Family #=GF RN [1] #=GF RM 1926776 #=GF RT Complete nucleotide sequence of the virus SSV1 of the #=GF RT archaebacterium Sulfolobus shibatae. #=GF RA Palm P, Schleper C, Grampp B, Yeats S, McWilliam P, Reiter WD, #=GF RA Zillig W; #=GF RL Virology 1991;185:242-250. #=GF DR INTERPRO; IPR012922; #=GF CC The sequences featured in this family are similar to a probable #=GF CC integrase (Swiss:P20214) expressed by the SSV1 virus of the #=GF CC archaebacterium Sulfolobus shibatae. This protein may be #=GF CC necessary for the integration of the virus into the host genome #=GF CC by a process of site-specific recombination [1]. #=GF SQ 17 #=GS Q980Y3_SULSO/40-112 AC Q980Y3.1 #=GS W7KM36_9CREN/1-47 AC W7KM36.1 #=GS ...
Immunoglobulin alignments in Sulfolobus tokodaii str. 7. Alignments can be refined by adding alignments from other genomes, adding your own sequences and/or aligning to other models from the same superfamily. The display of alignments can also be customised.
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we have characterized the structure and the function of the 6.6-kda protein svtr (formerly called gp08) from the rod-shaped virus sirv1, which infects the hyperthermophilic archaeon sulfolobus islandicus that thrives at 85 degrees c in hot acidic springs. the protein forms a dimer in solution. the nmr solution structure of the protein consists of a ribbon-helix-helix (rhh) fold between residues 13 and 56 and a disordered n-terminal region (residues 1-12). the structure is very similar to that of ...
Turriviridae is a family of viruses; it contains only one genus, Alphaturrivirus. The archaea Sulfolobus solfataricus serve as natural hosts. There are currently only two species in the genus Alphaturrivirus. Viruses in Turriviridae have icosahedral geometries, and T=31 symmetry. The diameter is around 74 nm. Genomes are linear. Viral replication is cytoplasmic. Entry into the host cell is achieved by adsorption into the host cell. DNA-templated transcription is the method of transcription. Sulfolobus solfataricus serves as the natural host. Transmission routes are passive diffusion. Viral Zone. ExPASy. Retrieved 13 August 2015. ICTV. Virus Taxonomy: 2014 Release. Retrieved 13 August 2015. Viralzone: Turriviridae ...
General Information: This strain was isolated from a hot spring on the Kamchatka Penninsula, in the Russian Far East. Hyperthermophilic acidophilic sulfur-metabolizing archeon. Sulfolobus islandicus is a thermo-acidophilic archeae commonly identified in hot, acidic sulfur springs. This organism can grow both chemoautotrophically, using sulfur or hydrogen sulfide, and heterotrophically. S. islandicus can play host to a number of plasmids and viruses which may be useful in developing tools for genetic analysis. In addition, Sulfolobus islandicus isolates from different areas in Russia, Iceland, and the United States have been shown to be genetically distinct from each other making this organism useful for comparative analysis. ...
Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER is expected to cite the literature specified by the DEPOSITOR ...
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Nicotinamide adenine dinucleotide phosphate (NAD(P)H)-flavin oxidoreductases (flavin reductases) catalyze the reduction of flavin by NAD(P)H and provide the reduced form of flavin mononucleotide (FMN) to flavin-dependent monooxygenases. Based on bioinformatics analysis, we identified a putative flavin reductase gene, sso2055, in the genome of hyperthermophilic archaeon Sulfolobus solfataricus P2, and further cloned this target sequence into an expression vector. The cloned flavin reductase (EC. 1.5.1.30) was purified to homogeneity and characterized further. The purified enzyme exists as a monomer of 17.8 kDa, free of chromogenic cofactors. Homology modeling revealed this enzyme as a TIM barrel, which is also supported by circular dichroism measurements revealing a beta-sheet rich content. The optimal pH for SSO2055 activity was pH 6.5 in phosphate buffer and the highest activity observed was at 120 degrees C within the measurable temperature. We showed that this enzyme can use FMN and flavin ...
TY - JOUR. T1 - Metabolism of pentose sugars in the hyperthermophilic archaea sulfolobus solfataricus and sulfolobus acidocaldarius. AU - Nunn, C E M. AU - Johnsen, U. AU - Schonheit, P. AU - Fuhrer, T. AU - Sauer, U. AU - Hough, David W. AU - Danson, Michael J. PY - 2010/10/29. Y1 - 2010/10/29. N2 - We have previously shown that the hyperthermophilic archaeon, Sulfolobus solfataricus, catabolizes D-glucose and D-galactose to pyruvate and glyceraldehyde via a non-phosphorylative version of the Entner-Doudoroff pathway. At each step, one enzyme is active with both C6 epimers, leading to a metabolically promiscuous pathway. On further investigation, the catalytic promiscuity of the first enzyme in this pathway, glucose dehydrogenase, has been shown to extend to the C5 sugars, D-xylose and L-arabinose. In the current paper we establish that this promiscuity for C6 and C5 metabolites is also exhibited by the third enzyme in the pathway, 2-keto-3-deoxygluconate aldolase, but that the second step ...
The central metabolic pathways are a glycolytic pathway, a pentose phosphate pathway, and the citric acid cycle (Fig. 1). Conversion of glucose to pyruvate via the nonphosphorylating Entner-Doudoroff pathway produces no net energy (19). Genes for most enzymes, except gluconate dehydratase, are present (Sso3204, 3197, 3194, 0666, 0913, 0981). Conversion of pentose substrates (xylose, arabinose) is predicted to proceed via the pentose phosphate pathway, or a variant thereof. However, only genes encoding ribose-5-P isomerase (Sso0978) and transketolase (Sso0297 and 0299) are assigned. In contrast, all citric acid cycle genes are present (Sso1077, 1095, 2182, 2356 to 2359, 2482, 2483, 2585, 2589, 2815, 2816, 2863).. It is striking that NAD+ is used rarely as an electron acceptor in some central metabolic redox reactions. Both glucose dehydrogenase and glyceraldehyde dehydrogenase are reported to reduce NADP+ specifically. Moreover, glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase, ...
Living organisms rely on many different mechanisms to adapt to changes within their environment. Protein phosphorylation and dephosphorylation events are one such way cells can communicate to generate a response to environmental changes. In the Kennelly laboratory we hope to gain insight on phosphorylation events in the domain Archaea through the study of the acidothermophilic organism Sulfolobus solfataricus. Such findings may provide answers into evolutionary relationships and facilitate an understanding of phosphate transfer via proteins in more elaborate systems where pathway disturbances can lead to disease processes. A λ-phage expression library was generated from S. solfataricus genomic DNA. The immobilized expression products were probed with a purified protein kinase, SsoPK4, and radiolabeled ATP to identify potential native substrates. A protein fragment of the ORF sso0563, the catalytic A-type ATPase subunit A (AtpA), was phosphorylated by SsoPK4. Full length and truncated forms of ...
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts extremozymes for utilization in White Biotechnology. Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012 ...
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts extremozymes for utilization in White Biotechnology. Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012 ...
Investigations were performed on the structural features responsible for kinetic thermal stability of a thermostable carboxypeptidase from the thermoacidophilic archaebacterium Sulfolobus solfataricus which had been purified previously and identified as a zinc metalloprotease [Colombo, DAuria, Fusi, Zecca, Raia and Tortora (1992) Eur. J. Biochem. 206, 349-357]. Removal of Zn2+ by dialysis led to reversible activity loss, which was promptly restored by addition of 80 microM ZnCl2 to the assay mixture. For the first-order irreversible thermal inactivation the metal-depleted enzyme showed an activation energy value of 205.6 kJ.mol-1, which is considerably lower than that of the holoenzyme (494.4 kJ.mol-1). The values of activation free energies, enthalpies and entropies also dropped with metal removal. Thermal inactivation of the apoenzyme was very quick at 80 degrees C, whereas the holoenzyme was stable at the same temperature. These findings suggest a major stabilizing role for the bivalent ...
1JUL: The crystal structure of indole-3-glycerol phosphate synthase from the hyperthermophilic archaeon Sulfolobus solfataricus in three different crystal forms: effects of ionic strength.
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Smeulders, M. J.; Barends, T.; Pol, A.; Scherer, A.; Zandvoort, M. H.; Udvarhelyi, A.; Khadem, A. F.; Menzel, A.; Hermans, J.; Shoeman, R. L. et al.; Wessels, H. J. C. T.; van den Heuvel, L. P.; Russ, L.; Schlichting, I.; Jetten, M. S. M.; Op den Camp, H. J. M.: Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Nature 478 (7369), pp. 412 - 416 (2011 ...
The reversible thermal unfolding of the archaeal histone-like protein Ssh10b from the extremophile Sulfolobus shibatae was studied using differential scanning calorimetry and circular dichroism spectroscopy. Analytical ultracentrifugation and gel filtration showed that Ssh10b is a stable dimer in the pH range 2.5-7.0. Thermal denaturation data fit into a two-state unfolding model, suggesting that the Ssh10 dimer unfolds as a single cooperative unit with a maximal melting temperature of 99.9 degrees C and an enthalpy change of 134 kcal/mol at pH 7.0. The heat capacity change upon unfolding determined from linear fits of the temperature dependence of DeltaH(cal) is 2.55 kcal/(mol K). The low specific heat capacity change of 13 cal/(mol K residue) leads to a considerable flattening of the protein stability curve (DeltaG (T)) and results in a maximal DeltaG of only 9.5 kcal/mol at 320 K and a DeltaG of only 6.0 kcal/mol at the optimal growth temperature of Sulfolobus.
The reversible thermal unfolding of the archaeal histone-like protein Ssh10b from the extremophile Sulfolobus shibatae was studied using differential scanning calorimetry and circular dichroism spectroscopy. Analytical ultracentrifugation and gel filtration showed that Ssh10b is a stable dimer in the pH range 2.5-7.0. Thermal denaturation data fit into a two-state unfolding model, suggesting that the Ssh10 dimer unfolds as a single cooperative unit with a maximal melting temperature of 99.9 degrees C and an enthalpy change of 134 kcal/mol at pH 7.0. The heat capacity change upon unfolding determined from linear fits of the temperature dependence of DeltaH(cal) is 2.55 kcal/(mol K). The low specific heat capacity change of 13 cal/(mol K residue) leads to a considerable flattening of the protein stability curve (DeltaG (T)) and results in a maximal DeltaG of only 9.5 kcal/mol at 320 K and a DeltaG of only 6.0 kcal/mol at the optimal growth temperature of Sulfolobus.
PDCD5蛋白質是個最早被發現在人類體內細胞用來上調節的細胞凋亡蛋白。PDCD5蛋白質可從細胞質向細胞核迅速的通過並與Tip60蛋白結合於DNA造成乙醯化,進而傳遞細胞凋亡訊息,其重要性為調節第五型賴氨酸乙酰轉化酶抑制其蛋白酶體相關的降解(參與轉錄、DNA損傷反應和細胞週期控制蛋白)。我們從KEGG數據分析庫確定了SSO0352基因為PDCD5蛋白在古生菌Sulfolobus solfataricus的同源基因,由於目前在古生菌Sulfolobus solfataricus的功能仍然還是未知,為了研究是否有相似的作用及結構。我們表現在質體pET-21a上和找到適合生產PDCD5蛋白質的方法,並表現於大腸桿菌(RIL)菌株,找到其純化條件。利用陽離子交換樹脂和膠體交換法純化出蛋白並使用X-ray繞射與單重原子異常散射(SAD)得知PDCD5蛋白的結構。Sso-PDCD5晶體在X-ray的繞射數據為Space group=C2, a = 100.34 Å, b = 39.71 Å, c = 77.57 ...
Lübben, M. , Güldenhaupt, J. , Zoltner, M. , Deigweiher, K. , Haebel, P. , Urbanke, C. and Scheidig, A. J. (2007): Sulfate Acts as Phosphate Analog on the Monomeric Catalytic Fragment of the CPx-ATPase CopB from Sulfolobus solfataricus , Journal of Molecular Biology ...
Project 1: Bipolar Tetraether Lipid Membranes: Physical Properties, and Technological Applications. The long-term goals of this research are to understand how the Archaea live in extreme environments and to use the Archaeal bipolar tetraether lipids for technological applications. The Archaea are curious and remarkable organisms; and, their lipids are structurally distinctly different from their bacterial and eukaryotic counterparts. The native habitat of the thermoacidophilic archaeon Sulfolobus acidocaldarius, which is the focus of this research, is hot (65-80 oC) and acidic (pH 2-3) sulfur springs. The plasma membrane of S. acidocaldarius not only serves as a barrier between the low pH extracellular environment and the neutral pH intracellular compartment (pH 6.5), but also performs proton pumping and other cellular activities at high temperatures. The ability of the plasma membrane to achieve these goals in extreme environments is not clearly understood, although there is evidence suggesting ...
In the archaeon Sulfolobus solfataricus the enzyme is involved in glucose and galactose catabolism via the branched variant of the Entner-Doudoroff pathway. It utilizes 2-dehydro-3-deoxy-6-phosphate-D-gluconate and 2-dehydro-3-deoxy-6-phosphate-D-galactonate with similar catalytic efficiency. In vitro the enzyme can also catalyse the cleavage of the non-phosphorylated forms 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate with much lower catalytic efficiency. cf. EC 4.1.2.21, 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, and EC 4.1.2.14, 2-dehydro-3-deoxy-phosphogluconate aldolase ...
However, Baas Becking has an answer for this; ...but the environment selects. What if the variation is due to selection acting at a finer scale? Its well established that species sorting effects play a major role in determining the composition of microbial communities at the species level. There is no particular reason to believe that this effect does not apply at smaller phylogenetic scales. The work with Sulfolobus islandicus attempts to control for this by choosing isolates from hot springs with similar physical and chemical properties, but unfortunately there is no such thing as a pair of identical hot springs. Just walk the boardwalks in Yellowstone, and youll see what I mean. The differences among the sites from which these microbes were isolated can always be offered as an alternative explanation to dispersal. Even if you crank those differences down to nearly zero, one can always suggest that perhaps there is a difference that we dont know about that happened to be important ...
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Typical growth inhibition of S. solfataricus on plates due to infectious virus. Lawns of S. solfataricus strain GΘ were prepared as in Stedman et al. (2003). T
Ammonia oxidizing archaea (AOA) of the phylum Thaumarchaeota are widespread in moderate environments but their occurrence and activity has also been
SWISS-MODEL Template Library (SMTL) entry for 1uwi.1. CRYSTAL STRUCTURE OF MUTATED BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS, WORKING AT MODERATE TEMPERATURE
I m looking for any info avaliable concerning maintenance of S. solfataricus. Please e-mail : dhatzini at orfeas.chemeng.ntua.gr ...
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How to upgrade SSO from 5.5 to 6.0 embedded to external. Single Sign-On to Platform Services controller internal multisite multimaster site recovery manager
43] Kouril T, Wieloch P, Reimann J, Wagner M, Zaparty M, Albers SV, Schomburg D, Ruoff P, Siebers B (2012). Unraveling the function of the two Entner-Doudoroff branches in the thermoacidophilic Crenarchaeon Sulfolobus solfataricus P2. FEBS J. 2012 Dec 24. doi: 10.1111/febs.12106. PMID:23279921. [42] Peetsch A, Greulich C, Braun D, Stroetges C, Rehage H, Siebers B, Köller M, Epple M (2012). Silver-doped calcium phosphate nanoparticles: Synthesis, characterization, and toxic effects toward mammalian and prokaryotic cells. Colloids Surf B Biointerfaces. 2013 Feb 1;102:724-9. doi: 10.1016/j.colsurfb.2012.09.040. Epub 2012 Oct 6. PMID:23107950. [41] Ulas T, Riemer SA, Zaparty M, Siebers B, Schomburg D (2012). Genome-scale reconstruction and analysis of the metabolic network in the hyperthermophilic archaeon Sulfolobus solfataricus. PLoS One. 2012;7(8):e43401. doi: 10.1371/journal.pone.0043401. Epub 2012 Aug 31. PMID:22952675. [40] Esser D, Pham TK, Reimann J, Albers SV, Siebers B, Wright PC (2012). ...
The CRISPR (clustered regularly interspaced short palindromic repeats) and Cas (CRISPR-associated) genes are widely spread in bacteria and archaea, representing an intracellular defence system against invading viruses and plasmids. In the system, fragments from foreign DNA are captured and integrated into the host genome at the CRISPR locus. The locus is transcribed and the resulting RNAs are processed by Cas6 into small crRNAs (CRISPR RNAs) that guide a variety of effector complexes to degrade the invading genetic elements. Many bacteria and archaea have one major type of effector complex. However, Sulfolobus solfataricus strain P2 has six CRISPR loci with two families of repeats, four cas6 genes and three different types of effector complex. These features make S. solfataricus an important model for studying CRISPR-Cas systems. In the present article, we review our current understanding of crRNA biogenesis and its effector complexes, subtype I-A and subtype III-B, in S. solfataricus. We also ...
Betalipothrixvirus is a genus of viruses in the order Ligamenvirales, in the family Lipothrixviridae. Archaea serve as natural hosts. There are currently six species in this genus including the type species Sulfolobus islandicus filamentous virus. Group: dsDNA Order: Ligamenvirales Family: Lipothrixviridae Genus: Betalipothrixvirus Acidianus filamentous virus 3 Acidianus filamentous virus 6 Acidianus filamentous virus 7 Acidianus filamentous virus 8 Acidianus filamentous virus 9 Sulfolobus islandicus filamentous virus Viruses in Betalipothrixvirus are enveloped, with rod-shaped geometries. The diameter is around 24 nm, with a length of 2000 nm. Genomes are linear, around 40.5kb in length. The genome has 67 open reading frames. Viral replication is cytoplasmic. Entry into the host cell is achieved by adsorption into the host cell. Dna templated transcription is the method of transcription. Archaea serve as the natural host. Transmission routes are passive diffusion. Viral Zone. ExPASy. ...
ID SULID_1_PE2489 STANDARD; PRT; 366 AA. AC SULID_1_PE2489; D2PGF4; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=eIF-2B alpha/beta/delta-related uncharacterized protein; DE (SULID_1.PE2489). GN OrderedLocusNames=LD85_2679; OS SULFOLOBUS ISLANDICUS L.D.8.5. OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Sulfolobus. OX NCBI_TaxID=425944; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS SULID_1.PE2489. CC Sulfolobus islandicus L.D.8.5 chromosome, complete genome. CC 1..88329 annotated by Ensembl Genomes CC -!- ANNOTATIONS ORIGIN:D2PGF4_SULID CC -!- SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits CC family. CC -!- GENE_FAMILY: HOG000224730 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; D2PGF4; -. DR EMBL; CP001731; ADB88294.1; -; Genomic_DNA. DR RefSeq; YP_003420664.1; NC_013769.1. DR ProteinModelPortal; D2PGF4; -. DR GeneID; ...
ID C4KE24_SULIK Unreviewed; 1008 AA. AC C4KE24; DT 07-JUL-2009, integrated into UniProtKB/TrEMBL. DT 07-JUL-2009, sequence version 1. DT 07-JUN-2017, entry version 43. DE RecName: Full=Tricorn protease homolog {ECO:0000256,PIRNR:PIRNR036421}; DE EC=3.4.21.- {ECO:0000256,PIRNR:PIRNR036421}; GN OrderedLocusNames=M164_0516 {ECO:0000313,EMBL:ACR41147.1}; OS Sulfolobus islandicus (strain M.16.4 / Kamchatka #3). OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Sulfolobus. OX NCBI_TaxID=426118 {ECO:0000313,EMBL:ACR41147.1, ECO:0000313,Proteomes:UP000001479}; RN [1] {ECO:0000313,EMBL:ACR41147.1, ECO:0000313,Proteomes:UP000001479} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=M.16.4 / Kamchatka #3 {ECO:0000313,Proteomes:UP000001479}; RX PubMed=19435847; DOI=10.1073/pnas.0808945106; RA Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J.; RT Biogeography of the Sulfolobus islandicus pan-genome.; RL Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009). CC -!- ...
SWISS-MODEL Repository entry for C3MZ62 (RISB_SULIA), 6,7-dimethyl-8-ribityllumazine synthase. Sulfolobus islandicus (strain M1627)
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Thermoacidophilic archaeon dehydrogenase. Computer model showing the structure of 3-isopropylmalate dehydrogenase from Sulfolobus acidocaldarius. - Stock Image C035/6196
Thermostable enzyme technology for algal bioconversion The present invention relates to thermostable enzyme systems suitable for use in the production of biofuels and bio-products from algae, and to a
Dorsal soft rays (total): 37; Anal soft rays: 33; Vertebrae: 43. Coronal pore present. Lower pectoral fin rays longer and thicker than upper lobe rays. Gill rakers unmodified. Coracoid half-moon shaped, helve absent. Mouth short, almost vertical, about 75° to horizontal. Skin pale, head beneath skin unpigmented (Ref. 43481). ...
E coli L12e protein: identified in E coli, Halobacterium cutirubrum and Sulfolobus solfataricus; amino acid sequence given in first source
Bebe Buell, the mother of Liv Tyler and Stiv Bators of The Dead Boys attend a Blondie party at Fiorucci in Los Angeles, California. **EXCLUSIVE** Get premium, high resolution news photos at Getty Images
Doom metal - rodzaj muzyki metalowej uznany za podgatunek w pierwszej połowie lat 80. Charakteryzuje się ciężkim i powolnym, zazwyczaj psychodelicznym stylem wokalnym oraz ciężkim, walcowatym brzmieniem; dominują średnie i wolne tempa. Teksty utworów poruszają tematy depresyjne, melancholijne, czasem określane jako dekadenckie. Muzycznie doom metal jest ściśle powiązany z wczesną twórczością Black Sabbath[1]. Utwory takie jak Black Sabbath i Into the Void uważane są za pierwsze, początkowe utwory nowego wówczas stylu. Od tego czasu zespoły takie jak Trouble, Saint Vitus, Candlemass, Pentagram, Katatonia, Anathema, Amber Tears oraz Witchfinder General zdefiniowały gatunek, a pierwszym, klasycznym albumem został Epicus Doomicus Metallicus zespołu Candlemass. ...