1GJM: Covalent Attachment of an Electroactive Sulphydryl Reagent in the Active Site of Cytochrome P450Cam as Revealed by the Crystal Structure of the Modified Protein
Definition of P-hydroxymercuribenzoate with photos and pictures, translations, sample usage, and additional links for more information.
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In chemistry, the limiting reagent is the reactant that is completely consumed in a chemical reaction. When the limiting reagent is gone, no more product can be formed -- and thats how this...
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This reaction is rapid and stoichiometric, with the addition of one mole of thiol releasing one mole of TNB. The TNB2− is quantified in a spectrophotometer by measuring the absorbance of visible light at 412 nm, using an extinction coefficient of 14,150 M−1 cm−1 for dilute buffer solutions,[3][4] and a coefficient of 13,700 M−1 cm−1 for high salt concentrations, such as 6 M guanidinium hydrochloride or 8 M urea.[4] Unfortunately the extinction coefficient for dilute solutions was underestimated in the original 1959 publication, as 13,600 M−1 cm−1, and as noted in a recent article, this mistake has persisted in the literature.[5] Commercial DTNB may not be completely pure, so may require recrystallization to obtain completely accurate and reproducible results.[4]. Ellmans reagent can be used for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood.[6] It can also measure the number of thiol groups on ...
Membrane-bound α-bungarotoxin-binding entities derived from rat brain are found to interact specifically with the affinity reagents maleimidobenzyltrimethylammonium (MBTA) and bromoacetylcholine (BAC), originally designed to label nicotinic acetylcholine receptors from electroplax and skeletal muscle. Following treatment of membranes with dithiothreitol, all specffic toxin binding sites are irreversibly blocked by reaction with MBTA or BAC. Affinity reagent labeling of dithiothreitol-reduced membranes is prevented (toxin binding sites are not blocked) by prior alkylation with N-ethylmaleimide, by prior oxidation with dithiobis(2-nitrobenzoic acid), or by incubation with neurotoxin. Reversibly associating cholinergic agonists and antagonists retard the rate of affinity reagent interaction with toxin receptors. The apparent rates of affinity reagent alkylation of toxin receptors, and the influences of other sulfhydryl/disulfide reagents on affinity labeling are comparable to those observed for ...
The most common schemes for forming a well-defined heteroconjugate involve the indirect coupling of an amine group on one biomolecule to a thiol group on a second biomolecule, usually by a two- or three-step reaction sequence.
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A heterogeneous specific binding assay method for determining the amount of a suspected analyte in an aqueous test medium wherein a reaction mixture is formed by combining the test medium with assay reagents including a labeled reagent, an immobilizable component, and a binding substance which causes the immobilizable component to precipitate. Free and bound species of the labeled reagent are formed as a function of the amount of the analyte in the test medium. One of the free and bound species of the labeled reagent is immobilized by binding of the immobilizable component with the binding substance. The immobilized labeled reagent is seperated from labeled reagent which has not been immobilized, and the amount of label in the labeled reagent in one of the separated fractions is determined and related to the amount of analyte in the test medium. The improvement provided is the use of an immobilizable component comprising a water dispersible, aggregatable reagent comprising a binding partner for the one
We have shown that the E172Q and E172C mutations of L247T α7 nicotinic receptors eliminate receptor stimulation by the presence of permeable divalent cations. ACh dose responses of E172Q/L247T α7 nAChRs were inhibited by the presence of Ca2+, Ba2+, or Sr2+, and E172C/L247T α7 nAChRs were insensitive to the presence of these ions. E172Q/L247T α7 nAChRs did not display the high level of basal activity that is a characteristic of L247T α7 receptors and were not activated by the antagonist DHβE. E172C/L247T α7 receptors were blocked by the thiol-modifying reagent MTSET, indicating that E172 is accessible to permeant ions. These data support the conclusion drawn from a chimera of α7 nicotinic receptors and 5-HT3 receptors that E172 is essential for the modulation of α7 receptors by Ca2+ and other permeant divalent cations (19). The data are also consistent with a model of α7 receptors based on the crystal structure of AChBP (6) that places E172 near the inner surface of the vestibule, where ...
Looking for nucleophilic reagent? Find out information about nucleophilic reagent. A reactant that gives up electrons, or a share in electrons, to other molecules or ions in the course of a chemical reaction Explanation of nucleophilic reagent
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(A) Beads of dried LAMP reagents in PCR tubes. (B) Feasibility data showing stability based on TTR of LAMP reagents in dried format stored at the indicated temp