TY - JOUR. T1 - Intramolecular crosslinking of hemoglobin A by sulfosuccinimidyl suberate. T2 - Application of crosslinked protein as a blood substitute. AU - Manjula, B. N.. AU - Acharya, A. S.. PY - 1994/11. Y1 - 1994/11. N2 - Sulfosuccinimidyl esters could be targeted to the residues of ββ cleft of hemoglobin A and the bis-sulfosuccinimidyl esters of aliphatic dicarboxylic acids could serve as the `affinity directed ββ crosslinkers of HbA. The reactivity of sulfosuccinimidyl esters of tartaric acid and sebacic acid with HbA was also investigated to establish the appropriate length of the alkyl chain optimal for the crosslinking reaction.. AB - Sulfosuccinimidyl esters could be targeted to the residues of ββ cleft of hemoglobin A and the bis-sulfosuccinimidyl esters of aliphatic dicarboxylic acids could serve as the `affinity directed ββ crosslinkers of HbA. The reactivity of sulfosuccinimidyl esters of tartaric acid and sebacic acid with HbA was also investigated to establish the ...
Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules,[1] notably, to intracellular lysine residues and other amine sources. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Also, due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. CFSE is commonly confused with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), although they are not strictly the same molecule; CFDA-SE, due to its acetate groups, is highly cell permeable, while CFSE is much less so. As CFDA-SE, which is non-fluorescent, enters the cytoplasm of cells, intracellular esterases remove the acetate groups and convert the molecule to the fluorescent ester. CFSE was originally developed as a fluorescent dye that could be used to stably label lymphocytes and track their migration within ...
Sulfo-NHS Crosslinker 500 mg $116.00 for bead conjugation, such as Luminex beads. Sulfo-NHS (N-hydroxysulfosuccinimide, CAS 106627-54-7) and EDC-HCl are used together in crosslinking reactions, and Sulfo-NHS improves conjugation efficiency. - Sulfo-NHS crosslinker (N-hydroxysulfosuccinimide) is used with EDC-HCl (EDAC-HCl) to convert carboxyl groups to Sulfo-NHS esters which react with primary amines. -
BS3 crosslinker packaged as one gram per vial. Bulk quantities of BS3 are also available from ProteoChem. - BS3 crosslinking reagent, Bis(sulfosuccinimidyl) suberate, is a water soluble, membrane impermeable, homobifunctional protein crosslinking reagent with an 8-atom non-cleavable spacer arm. -
BOC-Glycine N-hydroxysuccinimide ester, 99%, ACROS Organics™ 5g BOC-Glycine N-hydroxysuccinimide ester, 99%, ACROS Organics™ Amino Acids
215597-96-9,CCD No.:CCD00211814,Formula:C12 H10 N2 O14 S2 . 2 Na,MolWeight:516.323,Synonyms:BIS(SULFOSUCCINIMIDYL)SUCCINATE SODIUM SALT; Molecular Structure,Chemical Cloud Database
CD4|sup|+|/sup|CD25|sup|+|/sup|Foxp3|sup|+|/sup| Tregs control the immune response and maintain immune homeostasis. This study examined whether Tregs can affect mouse enteritis and the Foxp3 (Forkhead transcription factor) transcriptional pathway. Mouse CD4|sup|+|/sup|CD25|sup|+|/sup| Treg cells were labelled using CFSE (5,6-carboxyfluorescein diacetate succinimidyl ester) and transferred to enteritis model mice. The mice were randomly divided into an enteritis group, a Treg-infusion group, a Treg-inhibiting group, and a control group. Histopathology, ELISA, flow cytometry, western blot, immunohistochemistry, and immunofluorescence were performed. Our results demonstrated that CD4|sup|+|/sup|CD25|sup|+|/sup| Tregs were successfully transferred. The disease activity index (DAI) scores in the Tregs-infusion group were lower than those of the enteritis and Tregs-inhibiting groups. The number of goblet cells and inflammatory cells was reduced, and the levels of IL-1|i|β|/i|, TNF-|i|α|/i|, NO,
Animals. All mice were used at 6-8 weeks of age. C57BL/6 mice were purchased from the National Cancer Institute (Frederick, Maryland, USA). A breeding pair of lymphotoxin-α (LTα) knockout mice (on a mixed 129 × C57BL/6 background) were originally obtained from David Chaplin (Washington University, St. Louis, Missouri, USA) and have since been backcrossed for more than eight generations onto a C57BL/6 background. For some experiments, LTα knockout mice were splenectomized 2 weeks prior to immunization. Splenectomies were performed by staff of the Veterinarian Care Services at Yale University.. Antigens. L. major promastigotes of the WR309 substrain were maintained at 23°C in Schneiders Drosophila medium (GIBCO BRL; Invitrogen Corporation, Grand Island, New York, USA) supplemented with 20% FCS and 50 μg/ml gentamicin. For some experiments, L. major parasites were labeled with CFSE (5- and 6-carboxyfluorescein diacetate succinimidyl ester; Molecular Probes Inc., Eugene, Oregon, USA) using a ...
Shop a large selection of Protein Labelling Reagents products and learn more about Molecular Probes™ Alexa Fluor™ 750 NHS Ester (Succinimidyl Ester) 3 x 100μg
Acetic acid N-hydroxysuccinimide ester (CAS 14464-29-0) Market Research Report 2017 aims at providing comprehensive data on acetic acid n-hydroxysuccinimide
Purified 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* from Creative Biomart. 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* can be used for research.
A convenient one-pot approach for the synthesis of aryl sulfides through the coupling of thiols with Grignard reagents in the presence of N-chlorosuccinimide is described. The sulfenylchlorides were formed when thiols were treated with N-chlorosuccinimide, and the resulting sulfenylchlorides were then directly reacted with Grignard reagents to provide aryl sulfides in good to excellent yields under mild reaction conditions. Functional groups including ester, fluoro and chloro are tolerated by the reaction conditions employed. It is important to note that this method has a short reaction time (30 min in total), and represents an alternative approach for the synthesis of aryl sulfides over the existing protocols ...
Find quality suppliers and manufacturers of 6066-82-6(N-Hydroxysuccinimide) for price inquiry. where to buy 6066-82-6(N-Hydroxysuccinimide).Also offer free database of 6066-82-6(N-Hydroxysuccinimide) including MSDS sheet(poisoning, toxicity, hazards and safety),chemical properties,Formula, density and structure, solution etc.
Creative Peptides offers Z-L-Valine N-hydroxysuccinimide ester for your research. We also provide custom peptide synthesis, process development, GMP manufacturing.
Chemical cross-linking was used to analyze the binding sites for the agonist bradykinin (BK) and the antagonists NPC17731 and HOE140 on the bovine B2 bradykinin receptor. [3H]BK and [3H]NPC17731 bound with high affinity to the same B2 receptor in bovine myometrial membranes as determined by the total number of specific binding sites and pharmacological specificity of the binding of these two radioligands. Cross-linking experiments were done using a series of bifunctional reagents reactive either primarily to amines (homobifunctional) or reactive to amines in one end and to sulfhydryls in the opposite end (heterobifunctional). All the heterobifunctional reagents plus the homobifunctional arylhalide 1,5-difluoro-2,4-dinitrobenzene were effective in cross-linking the [3H]BK N terminus specifically to a sulfhydryl in the receptor, and this cross-linking occurred at 5-100 μM reagent. In contrast, the homobifunctional N-hydroxysuccinimide ester reagents, at ≤1 mM, were only able to cross-link ...
ECHA compiled an inventory of substances likely to meet the criteria of Annex III to the REACH Regulation. The aim is to support registrants in identifying whether reduced minimum information requirements or a full Annex VII information set is required.. The inventory was produced using publicly available databases with experimental data and by using (Q)SAR model results. Indications for hazardous toxicological or ecotoxicological properties together with information on uses and other available relevant information have to be compared with the criteria in Annex III.. The fact that a substance is not in this list does not necessarily mean that the criteria for Annex III are not met. Likewise, if a substance is on this inventory, a registrant can still benefit from the reduced information requirements if it is justified.. Note that the inventory is not a tool for classification, it only shows indications for concern. For instance, the fact that a substance is indicated as Suspected mutagen does ...
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Shop a large selection of Crosslinking Reagents products and learn more about Thermo Scientific DSS (disuccinimidyl suberate), No-Weigh Format 50mg:Life 50mg.
Fmoc-N-amido-dPEG®8-NHS ester, product number 10995, is one of Quanta BioDesigns peptide synthesis products. This product is pre-activated with an N-hydroxysuccinimide (NHS) ester for conjugation to free amines. The dPEG®8 spacer allows the introduction of a short, hydrophilic spacer into a peptide chain. Read More PEGylation with Quanta BioDesign's dPEG® Products PEGylation refers to the covalent addition of polyethylene glycol (PEG) to a compound or surface. PEGylated compounds include proteins, peptides, dyes or other labels, and small molecule drugs. Peptide synthesis frequently employs PEGylation to enhance the water solubility of hydrophobic peptides. Moreover, peptide PEGylation prolongs the in vivo circulation of peptide conjugates by both increasing the peptide's hydrodynamic volume and protecting the peptide from proteolysis. Additionally, PEGylation reduces or eliminates the antigenicity of the conjugated peptide. Traditional PEGs, however, are dispersed polymers (Đ | 1
Materials. Bis(sulfosuccinimidyl)suberate (BS3), disuccinimidyl suberate (DSS), and NHS-SS-biotin were obtained from Pierce (Rockford, IL). Potassium hydroxide (99%) was from Aldrich (Milwaukee, WI). Type IV collagen was from Collaborative Biomedical Products (Bedford, MA). Normal goat serum and Vectastain mounting medium were from Vector Laboratories (Burlingame, CA). Except as noted, Sigma (St. Louis, MO) was the source of all other reagents and chemicals.. NGF was prepared as described previously (Mobley et al., 1986). NGF was labeled with 125I (Amersham, Arlington Heights, IL) using lactoperoxidase, as modified from Vale and Shooter (1985). Iodinated protein was separated from free 125I on a PD-10 column (Pharmacia, Uppsala, Sweden) preequilibrated with binding buffer [PBS, pH 7.4, containing 1 mg/ml glucose and 1 mg/ml bovine serum albumin (BSA)]. Final specific activity was 25-100 cpm/pg. Radioactivity was quantified using a Beckman 2000 gamma counter.. 1088 is a rabbit antibody against ...
Despite great advances in immunological research during the last decades, estimating the kinetics of lymphocyte populations has proved quite difficult. There are widely divergent estimates of the production rates, division rates and lifespans of mouse and human lymphocyte populations [1]. As a consequence, it is poorly understood how memory is maintained, how the naive lymphocyte repertoire remains diverse and how homeostasis is brought about. Additionally, it remains poorly understood how viruses such as HIV, and diseases such as rheumatoid arthritis, disturb the normal population dynamics and deteriorate the functioning of the immune system.. Several in vivo labelling techniques have been developed that have enabled the generation of quantitative data on lymphocyte dynamics. Examples of labels are the fluorescent dye carboxy-fluorescein diacetate succinimidyl ester (CFSE), the base analogue 5-bromo-2′-deoxyuridine (BrdU) and the stable isotype deuterium. Major advantages of deuterium ...
BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX (Texas Red). The NHS ester function can be used for the conjugation with proteins and peptides.
Rates of Decomposition of N-Chloramine Disinfectant Compounds in Aqueous Solutions. International Nuclear Information System (INIS) EI-Bellihi, E.E.. 2009-01-01. The effect of temperature, ph, and salt effects on the decomposition kinetics of hydrolysis of N-chloramine disinfectant compounds [chloramine-B, chloramine-T, N-chlorosuccinimide (NCS), and 1,3-dichloro-5,5-dimethyl hydantoin (DCDMH or Halane)] in aqueous solutions was studied. The results should that the hydrolytic stability of CB and CT is greater than that of NCS and halane. Using CT, which is practical in use for its long contact times, reduced its initial concentration in aqueous solution from 100 ppm to about 20 ppm after a period of 6 months. The study also showed that the rate of hydrolysis of NCS is almost independent on the H + ions concentration. On the other hand, the rates of hydrolysis of CB and CT depend strongly on the hydrogen ion (H + ) concentration where the kinetic of the reaction changes from zero-order to a first ...
Authors: ASGHAR DAVOOD, ESKANDAR ALIPOUR, ABBAS SHAFIEE Abstract: 4(5)-Chloro-imidazole-5(4)-carboxaldehyde derivatives are important precursors for the preparation of biologically active compounds. We developed a simple, novel, and efficient method for the synthesis of these compounds. The chemistry described is amenable to large-scale use and is flexible enough to allow the preparation of analogs. Keywords: Imidazole, N-chlorosuccinimide, chloroimidazole, chloroimidazole-carboxaldehyde Full Text: PDF ...
Photoreactive insulin analogues specifically label predominantly one polypeptide in the insulin receptor of rat liver plasma membranes. We have used the bifunctional reagent disuccinimidyl suberate to cross-link this polypeptide to its neighbouring, but not necessarily labelled, subunits. The results of these studies show that (1) there are at least three types of subunit in the receptor, with apparent Mr (Mapp.) values of 65 000, 95 000 and 120 000; (2) the receptor appears to consist of two Mapp. 120 000, one Mapp. 95 000 and one Mapp. 65 000 subunits; (3) the Mapp. 65 000 subunit, which has not been previously reported, may be only loosely attached to the receptor, and does not interact directly with the insulin-binding subunit (M app. 120 000). ...
Alfa Aesar™ N-Boc-L-phenylalanine N-succinimidyl ester, 98% 250mg Alfa Aesar™ N-Boc-L-phenylalanine N-succinimidyl ester, 98% Amino Acids
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dyes functional group. See the product information sheets for specific reactive dyes more information.. Coelenterazines and D-luciferin. Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also ...
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Can replace Alexa Fluor 647, DyLight 649 ...
产品名称:Acid-PEG8-NHS ester货号:BAPN-7品牌:BioconeCAS: 分子式: C24H41NO14分子量: 567.59纯度:95% 以上 包装规格:100 mg、500mg、1g、5g、10g等,其...
IR Fluor 680 (IRDye® 680 equivalent) is a bright, near-IR fluorescent, amine-reactive dyes used for labeling primary amines of proteins.
To assess the efficacy of their method, the team mixed the aldehydes with brain cancer cells in vitro for 10 minutes then compared them with typical amino reactive dye precursors known as succinimidyl esters (NHS) (Fig. 1). They discovered that the aldehyde precursors produced brighter fluorescence than the NHS dyes. Confocal microscopy showed that the aldehydes reacted with amino groups of lysine amino acid residues on the cell surface and those of other cell membrane components, whereas the NHS dyes penetrated the cells. They confirmed this by treating the labeled cells with detergent: the aldehyde-derived labels washed off the cell surface, whereas their NHS counterparts remained in the cells. The aldehyde-derived labels also remained effective at exceptionally low concentrations, unlike the NHS-derived labels. In contrast to pre-existing cell labeling protocols, this reaction tightly anchors the labels to the surface of living cells within 10 minutes at 10 nM [dye] concentrations and with a ...
Fluorescent Dyes , Reactive Fluorescent Dyes , 5(6)-TAMRA-X, SE; TAMRA-X contains a seven-atom aminohexanoyl spacer (known as X ) between TAMRA fluorophore and the succinimidyl ester. The X spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. We recommend this TAMRA-X derivative as the preferred dye for preparing TAMRA-labeled proteins when the fluorescence quenching of the labeling dye by protein is a serious problem.; C35H36N4O8
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Product Name: Propargyl-PEG6-NHS esterFormula: C20H31NO10MW: 445.5Web Site:MedchemexpressPurity: 0.98Availability: In StockCAS NO: 1403254-99-8 Product: EPZ-6
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Shop a large selection of Cell Growth and Differentiation Reagents and Kits products and learn more about Invitrogen™ CellTrace™ CFSE Cell Proliferation Kit,
VivoTag-S 750 amine-reactive near-infrared fluorochrome for coupling via an NHS ester linkage to peptides, small molecules, proteins, antibodies or macromolecules. 5 mg quantity.
Mesenchymal stem cells (MSCs) have been shown to alleviate acute lung injury (ALI) and induce the production of regulatory dendritic cells (DCregs), but the potential link between these two cell types remains unclear. The goal of this study was to investigate the effect and mechanism of MSC-induced regulatory dendritic cells in ALI mice. In vivo experiments, C57BL/6 wild-type male mice were sacrificed at different times after intratracheal injection of LPS to observe changes in lung DC maturation and pathological damage. MSCs, DCregs or/and carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled DCs were administered to the mice by tail vein, and flow cytometry was performed to measure the phenotype of lung DCs and T cells. Lung injury was estimated by the lung wet weight/body weight ratio and histopathological analysis. In vitro, Western blotting or flow cytometry was used to detect the expression of Notch ligand or receptor in MSCs or DCs after coculture or LPS stimulation. Finally, in vivo and
Objective: Metal alloys used in dentistry and in other biomedical fields may release nickel ions in the oral environment. The release of nickel might influence the normal biological and physiological processes, including tissue wound healing, cell growth and proliferation. The aim of this study was to evaluate in vitro the effects of nickel ions on cell cycle, viability and proliferation. Materials and Methods: Human osteosarcoma cells (U2OS) and human keratinocytes (HaCat) were exposed to different nickel chloride (NiCl2) concentrations (0 - 5mM) for various periods exposure. The viability of cultured cells was estimated by flow cytometry using Annexin V-FITC and Propidium Iodide (PI). Cell proliferation was evaluated by using carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and flow cytometry. Finally, the effects of NiCl2 on cell cycle were assessed and quantified by flow cytometry. Statistical analysis was performed by means of ANOVA followed by Tukeys test. Results: NiCl2 induced ...
In proteins and peptides, d-aspartic acid (d-Asp) and d-β-Asp residues can be spontaneously formed via racemization of the succinimide intermediate formed from l-Asp and l-asparagine (l-Asn) residues. These biologically uncommon amino acid residues are known to have relevance to aging and pathologies. Although nonenzymatic, the succinimide racemization will not occur without a catalyst at room or biological temperature. In the present study, we computationally investigated the mechanism of succinimide racemization catalyzed by dihydrogen phosphate ion, H2PO4−, by B3LYP/6-31+G(d,p) density functional theory calculations, using a model compound in which an aminosuccinyl (Asu) residue is capped with acetyl (Ace) and NCH3 (Nme) groups on the N- and C-termini, respectively (Ace-Asu-Nme). It was shown that an H2PO4− ion can catalyze the enolization of the Hα-Cα-C=O portion of the Asu residue by acting as a proton-transfer mediator. The resulting complex between the enol form and H2PO4− corresponds to
Haemopexin receptors from mouse hepatoma (Hepa) cells were affinity-labelled by cross-linking to haem-125I-haemopexin complexes using two homo-[disuccinimidyl suberate (DSS) and 3,3′-dithiobis(succinimidyl propionate) (DTSSP)] and one hetero-[sulphosuccinimidyl 4-(p-maleimidophenyl)butyrate (sulpho-SMPB)] bifunctional cross-linking agents. Analysis of the cross-linked products by SDS/PAGE in the absence of reducing agents revealed that 125I-haemopexin was cross-linked specifically to a protein of apparent molecular mass 85-90 kDa. Upon reduction, haemopexin remained cross-linked to a protein of 20 kDa, suggesting that the murine haemopexin receptor has a subunit structure. Two subunits were identified: alpha (p65) and beta (p20). Furthermore, because haemopexin was cross-linked by all three agents to p20, the shortest cross-linker arm being 1.1 nm (11 A), we propose that the haem-haemopexin-binding site resides on this subunit. In addition, a cysteine residue of p20 is located near the ...
Background: Sublingual immunotherapy (SLIT) efficacy could be improved by formulations facilitating allergen contact with the oral mucosa and uptake by antigen-presenting cells (APCs).. Methods: Two types of chitosan microparticles, differing in size and surface charge, were tested in vitro for their capacity to improve antigen uptake and presentation by murine bone marrow-derived dendritic cells (BMDCs) or purified oral APCs. T-cell priming in cervical lymph nodes (LNs) was assessed by intravenous transfer of carboxyfluorescein diacetate succinimidyl ester-labelled ovalbumin (OVA)-specific CD4+ T cells and flow cytometry analysis. Ovalbumin-sensitized BALB/c mice were treated sublingually with soluble or chitosan-formulated OVA twice a week for 2 months. Airway hyperresponsiveness (AHR), lung inflammation and T-cell responses in cervical and mediastinal LNs were assessed by whole-body plethysmography, lung histology and Cytometric Bead Array technology, respectively.. Results: Only a ...
Rivotril belongs to a class of drugs known as benzodiazepines .Rivotril is used alone or along with other medications to treat convulsive disorders such as epilepsy. Clonazepam is metabolized by the liver to inactive metabolites, which are excreted mainly in the urine. It is also prescribed for panic disorder--unexpected attacks of overwhelming panic accompanied by fear of recurrence.. I n this multicenter, parallel-group, placebo-controlled, fixed-dose study, the efficacy, safety, dosing characteristics, and discontinuation of clonazepam were analyzed in patients with panic disorder.. Rivotril is useful alone or as an adjunct in the treatment of the Lennox-Gastaut syndrome (petit mal variant), akinetic and myoclonic seizures. In patients with absence seizures (petit mal) who have failed to respond to succinimides, Rivotril may be useful. ...
Abstract:. Background: Biomass extract of Streptomyces sanyensis (BE-SS), which is a marine microorganism, has antitumor activity and has white-to-gray aerial mycelium and long chains of long spores in aerial mycelium. Objectives: The anti-cancer effect of BE-SS on triple-negative breast cancer (TNBC) was confirmed and cell death by BE-SS was confirmed to have immunogenicity and whether it could be used for immuno-cancer therapy. Materials and Methods: Human and mouse TNBC cells (MDA-MB-231, HS578T, 4T1-hemagglutinin (HA) and TUBO-P2J-HA cells) were treated with BE-SS at different concentrations for 72 hr and their cytotoxicity was detected using the sulforhodamine B-based (SRB) method. Flow cytometry was used to determine the type of cell death by Annexin V/7-AAD staining and to measure surface exposure to damage-related molecular pattern (DAMP) molecules including calreticulin (CRT), heat shock protein (HSP) 70/90. Carboxyfluorescein succinimidyl ester (CFSE) dilution assay was used to ...
Treatment of quiescent cultures of mouse embryo fibroblasts with 20% fetal calf serum (FCS) or cycloheximide (CH) resulted in the induction of a nuclear protein of molecular weight 29 000 daltons. The 29 Kd protein induced by these two agents was found to be tightly bound to the chromatin since it was not released from the chromatin by a combination of 2.5 M NaCl and 3.2 M urea. The chromatin associated CH-induced 29 Kd protein and the 29 Kd protein obtained from serum-induced cells displayed similar N-chlorosuccinimide cleavage patterns. Pulse-chase experiments indicate a half-life of an hour for the 29 Kd protein. The kinetics of induction of the 29 Kd protein in the early hours of mitogen addition, short half-life, nuclear localisation and strong association with chromatin suggest that this protein may have important roles in cell proliferation, possibly as a mediator of mitogen action. ...
Yes, methsuximide may be given to children. Methsuximide (Celontin) is an anticonvulsant medication that controls absence seizures by decreasing the a
This invention is to a fuel oil composition containing polyolefinic succinimide polyamine alkyl acetoacetate adducts of the general following: ##STR1## wherein the variables are defined herein. The adduct additives are especially useful in concentrates and fuel oil compositions as dispersants. The formulations have excellent cold flow properties compared to similar formulations not containing the inventive adduct.
The activation of G protein-coupled receptors (GPCR) mediates a variety of important biological signals. GPR110 belongs to the adhesion GPCR class with distinctively long N-terminus. Although GPR110 is an orphan receptor with unknown ligand, it has been identified as an oncogene implicated in lung and prostate cancers. In an effort to understand the molecular basis of GPR110 activation, we probed GPR110 conformation in living cells using chemical crosslinking and mass spectrometry. HEK cells overexpressing HA-GPR110 were incubated with disuccinimidyl suberate (DSS, a lysine-specific crosslinker). The DSS-modified HA-GPR110 was pulled-down and subjected to SDS-PAGE, tryptic digestion, and nanoLC-ESI-MS/MS. The MS-based approach identified 17 crosslinked lysine pairs in the N-terminal domain of GPR110. Among them, K29-K38, K187-K240, K240-K254, K398-442, K398-K438, K398-K427, K427-K442, K427-K438, and K151-K442, resulted from through-space crosslinking between two peptide segments, while K31-K32, ...
Sequence Modifiers are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, N2-Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labeled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide. O ur repertoire of NHS ester derivatives has been expanded to include the NHS-Carboxy-dT-CE Phosphoramidite. By making a dT analog of the ...
4] a ) J. T. Kauer, S. Erickson-Vitanen, H. R. Wolfe, W. F. De Grddo, J B d . Chrm. 1986.261. 10695, b) W. T. Miller, E. T. Kaiser. Proc. Nurl. Arud. Sci. U S A 1988, K S , 5429. c) T. Tao. C. J. Schemer, M. Lamkin. 5io~liemistr.v 1986.2.7. 7633. [5] a ) R. Breslow, Acr. Chrm. Res. 1980, 13, 170, b) C . Helene, Phofochem. Pholohiol. 1972, 16. 519. [6] G. W. Anderson, J. E. Zimmerman. F. M. Gallahan, J. Am. Cheni. Sor. 1964. K6. 1839. [7] A section of a silica substrate bearing nitroveratryloxycarbonyl(NV0C)protected amino groups was photodeprotected [3] and then derivatized uith the NHS ester of 3-benzoylbenzoic acid. A second section of the substrate was then deprotected and derivatized with the NHS ester of biotin. Any unreacted sites in the first section (due to incomplete coupling of benzophenone) will also react with the biotin NHS ester. The entire surface was then treated with fluorescein-streptavidin (Molecular Probes lnc.. Eugene. O R (USA)). The coupling efficiency of benzophenone was ...
PEG PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. It can be used either in homobifunctional or heterobifunctional labelling such as surface modification on nanoparticles and cells ...
Fluorescent labelling of monoclonal antibodies (mAbs) is classically performed by chemical bioconjugation methods. The most frequent labelling technique to generate antibody-fluorophore conjugates (AFCs) involves the bioconjugation onto the mAb lysines of a dye bearing an N-hydroxysuccinimide ester or an isothiocyanate group. However, discrepancies between labelling experiments or kits can be observed, related to reproducibility issues, alteration of antigen binding, or mAb properties. The lack of information on labelling kits and the incomplete characterization of the obtained labelled mAbs largely contribute to these issues. In this work, we generated eight AFCs through either lysine or interchain cysteine cross-linking bioconjugation of green-emitting fluorophores (fluorescein or BODIPY) onto either trastuzumab or rituximab. This strategy allowed us to study the influence of fluorophore solubility, bioconjugation technology, and antibody nature on two known labelling procedures. The structures of
Beyond providing Skin Deep® as an educational tool for consumers, EWG offers its EWG VERIFIED™ mark as a quick and easily identifiable way of conveying personal care products that meet EWGs strict health criteria. Before a company can use EWG VERIFIEDTM on such products, the company must show that it fully discloses the products ingredients on their labels or packaging, they do not contain EWG ingredients of concern, and are made with good manufacturing practices, among other criteria. Note that EWG receives licensing fees from all EWG VERIFIED member companies that help to support the important work we do. Learn more , Legal Disclaimer ...
AnaTag Protein Labeling Kits , Classic Fluorescent Dye Labeling Kits , AnaTag AMCA - X Microscale Protein Labeling Kit *Ultra Convenient*; AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group. This spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. AMCA fluorescence can be detected at the emission wavelength of 442 10 nm when excited at 353 10 nm. The AMCA-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the AMCA-protein conjugates. The kit is able to perform three conjugation reactions with each reaction
AnaTag™ HiLyte Fluor™ Protein Labeling kits provide a way to label proteins using the succinimidyl ester reactive form of the HiLyte Fluor™ dyes.
Surfaces with primary or secondary amino groups covalently bound are dedicated to promote the covalent immobilisationof compounds containing reactive moieties such as amino, carboxyl or thiol groups via well-known homoheterobifunctionallinkers, e.g. N-Hydroxysuccinimide (NHS) or Succinimidyl 4-(N-maleidomethyl) cyclohexane-1-carboxylate (SMCC).. This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules tothe surfaces:. ...
Surfaces with primary or secondary amino groups covalently bound are dedicated to promote the covalent immobilisationof compounds containing reactive moieties such as amino, carboxyl or thiol groups via well-known homoheterobifunctionallinkers, e.g. N-Hydroxysuccinimide (NHS) or Succinimidyl 4-(N-maleidomethyl) cyclohexane-1-carboxylate (SMCC).. This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules tothe surfaces:. ...
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Oral tolerance is a phenomenon that may occur in animals exposed to protein antigens for the first time by the oral route. They become unable to produce immune responses at the levels normally observed when they are immunized parenterally with antigen in the presence of adjuvants. Lipids have been used as adjuvants for both parenteral and oral immunization. In the present study we coupled ovalbumin with palmitate residues by incubating the protein with the N-hydroxysuccinimide palmitate ester and tested the preparation for its ability to induce oral tolerance. This was performed by giving 20 mg of antigen to mice by the oral route 7 days prior to parenteral immunization in the presence of Al(OH)3. Mice were bled one week after receiving a booster that was ...
Preparation of morphine-N-(ε-trifluoroacetylcaproyloxy) succinimide ester (TFCS)-keyhole limpet hemocyanin (KLH), showing conjugation of 6-glutarylmorphine (M-
Find more than 1700 high purity PEG linkers with a broad selection of funcitonal groups (amine, azide, maleimide, NHS ester, bromide, etc) to inspire your PEGylation. Order now!
View and buy high quality BDY 630-X, SE from Tocris Bioscience. Red BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester.
Reactive PromoFluor dyes (available as e.g. NHS esters or maleimides) for labeling of biomolecules such as proteins, antibodies and nucleic acids.
Page contains details about carboxyfluorescein loaded bolaamphiphilic nanovesicle . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Highly hydrophilic, amine-reactive label containing one NHS-ester group. A brighter and more photostable replacement for Alexa 633, with a much higher extinction coefficient (250,000) and brightness after labeling to proteins. ...
The Spectrolinker XL-1000 UV crosslinker was made for applications including eliminating PCR contamination, cross-linking DNA an...
When a low concentrations of crosslinker is added to resist (a film used to lay down the patterns of ever-shrinking lines and features on a chip, shown here at left), it is able to pattern smaller ...