TY - JOUR. T1 - Intramolecular crosslinking of hemoglobin A by sulfosuccinimidyl suberate. T2 - Application of crosslinked protein as a blood substitute. AU - Manjula, B. N.. AU - Acharya, A. S.. PY - 1994/11. Y1 - 1994/11. N2 - Sulfosuccinimidyl esters could be targeted to the residues of ββ cleft of hemoglobin A and the bis-sulfosuccinimidyl esters of aliphatic dicarboxylic acids could serve as the `affinity directed ββ crosslinkers of HbA. The reactivity of sulfosuccinimidyl esters of tartaric acid and sebacic acid with HbA was also investigated to establish the appropriate length of the alkyl chain optimal for the crosslinking reaction.. AB - Sulfosuccinimidyl esters could be targeted to the residues of ββ cleft of hemoglobin A and the bis-sulfosuccinimidyl esters of aliphatic dicarboxylic acids could serve as the `affinity directed ββ crosslinkers of HbA. The reactivity of sulfosuccinimidyl esters of tartaric acid and sebacic acid with HbA was also investigated to establish the ...
Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules,[1] notably, to intracellular lysine residues and other amine sources. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Also, due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. CFSE is commonly confused with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), although they are not strictly the same molecule; CFDA-SE, due to its acetate groups, is highly cell permeable, while CFSE is much less so. As CFDA-SE, which is non-fluorescent, enters the cytoplasm of cells, intracellular esterases remove the acetate groups and convert the molecule to the fluorescent ester. CFSE was originally developed as a fluorescent dye that could be used to stably label lymphocytes and track their migration within ...
BOC-Glycine N-hydroxysuccinimide ester, 99%, ACROS Organics™ 5g BOC-Glycine N-hydroxysuccinimide ester, 99%, ACROS Organics™ Amino Acids
DSP crosslinker (Dithiobis[succinimidyl propionate]; DTSP) is a homobifunctional protein crosslinker that is cell membrane permeable. DSP has amine-reactive NHS esters at both ends of a cleavable, 8-atom (12.0 angstrom) spacer arm.
BS3-d4 deuterated crosslinker 50 mg $459.00 - Bis(Sulfosuccinimidyl) 2,2,7,7-suberate-d4 (BS3-d4) dueterated protein crosslinker is a water soluble, membrane impermeable crosslinking agent isotopically labeled with 4 deuterium atoms to provide a 4 dalton shift by mass spec analysis. -
215597-96-9,CCD No.:CCD00211814,Formula:C12 H10 N2 O14 S2 . 2 Na,MolWeight:516.323,Synonyms:BIS(SULFOSUCCINIMIDYL)SUCCINATE SODIUM SALT; Molecular Structure,Chemical Cloud Database
Animals. All mice were used at 6-8 weeks of age. C57BL/6 mice were purchased from the National Cancer Institute (Frederick, Maryland, USA). A breeding pair of lymphotoxin-α (LTα) knockout mice (on a mixed 129 × C57BL/6 background) were originally obtained from David Chaplin (Washington University, St. Louis, Missouri, USA) and have since been backcrossed for more than eight generations onto a C57BL/6 background. For some experiments, LTα knockout mice were splenectomized 2 weeks prior to immunization. Splenectomies were performed by staff of the Veterinarian Care Services at Yale University.. Antigens. L. major promastigotes of the WR309 substrain were maintained at 23°C in Schneiders Drosophila medium (GIBCO BRL; Invitrogen Corporation, Grand Island, New York, USA) supplemented with 20% FCS and 50 μg/ml gentamicin. For some experiments, L. major parasites were labeled with CFSE (5- and 6-carboxyfluorescein diacetate succinimidyl ester; Molecular Probes Inc., Eugene, Oregon, USA) using a ...
... aims at providing comprehensive data on acetic acid n-hydroxysuccinimide
Purified 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* from Creative Biomart. 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* can be used for research.
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Despite great advances in immunological research during the last decades, estimating the kinetics of lymphocyte populations has proved quite difficult. There are widely divergent estimates of the production rates, division rates and lifespans of mouse and human lymphocyte populations [1]. As a consequence, it is poorly understood how memory is maintained, how the naive lymphocyte repertoire remains diverse and how homeostasis is brought about. Additionally, it remains poorly understood how viruses such as HIV, and diseases such as rheumatoid arthritis, disturb the normal population dynamics and deteriorate the functioning of the immune system.. Several in vivo labelling techniques have been developed that have enabled the generation of quantitative data on lymphocyte dynamics. Examples of labels are the fluorescent dye carboxy-fluorescein diacetate succinimidyl ester (CFSE), the base analogue 5-bromo-2′-deoxyuridine (BrdU) and the stable isotype deuterium. Major advantages of deuterium ...
... is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX (Texas Red). The NHS ester function can be used for the conjugation with proteins and peptides.
Authors: ASGHAR DAVOOD, ESKANDAR ALIPOUR, ABBAS SHAFIEE Abstract: 4(5)-Chloro-imidazole-5(4)-carboxaldehyde derivatives are important precursors for the preparation of biologically active compounds. We developed a simple, novel, and efficient method for the synthesis of these compounds. The chemistry described is amenable to large-scale use and is flexible enough to allow the preparation of analogs. Keywords: Imidazole, N-chlorosuccinimide, chloroimidazole, chloroimidazole-carboxaldehyde Full Text: PDF ...
Photoreactive insulin analogues specifically label predominantly one polypeptide in the insulin receptor of rat liver plasma membranes. We have used the bifunctional reagent disuccinimidyl suberate to cross-link this polypeptide to its neighbouring, but not necessarily labelled, subunits. The results of these studies show that (1) there are at least three types of subunit in the receptor, with apparent Mr (Mapp.) values of 65 000, 95 000 and 120 000; (2) the receptor appears to consist of two Mapp. 120 000, one Mapp. 95 000 and one Mapp. 65 000 subunits; (3) the Mapp. 65 000 subunit, which has not been previously reported, may be only loosely attached to the receptor, and does not interact directly with the insulin-binding subunit (M app. 120 000). ...
Alfa Aesar™ N-Boc-L-phenylalanine N-succinimidyl ester, 98% 250mg Alfa Aesar™ N-Boc-L-phenylalanine N-succinimidyl ester, 98% Amino Acids
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dyes functional group. See the product information sheets for specific reactive dyes more information.. Coelenterazines and D-luciferin. Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also ...
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Can replace Alexa Fluor 647, DyLight 649 ...
To assess the efficacy of their method, the team mixed the aldehydes with brain cancer cells in vitro for 10 minutes then compared them with typical amino reactive dye precursors known as succinimidyl esters (NHS) (Fig. 1). They discovered that the aldehyde precursors produced brighter fluorescence than the NHS dyes. Confocal microscopy showed that the aldehydes reacted with amino groups of lysine amino acid residues on the cell surface and those of other cell membrane components, whereas the NHS dyes penetrated the cells. They confirmed this by treating the labeled cells with detergent: the aldehyde-derived labels washed off the cell surface, whereas their NHS counterparts remained in the cells. The aldehyde-derived labels also remained effective at exceptionally low concentrations, unlike the NHS-derived labels. "In contrast to pre-existing cell labeling protocols, this reaction tightly anchors the labels to the surface of living cells within 10 minutes at 10 nM [dye] concentrations and with a ...
Fluorescent Dyes , Reactive Fluorescent Dyes , 5(6)-TAMRA-X, SE; TAMRA-X contains a seven-atom aminohexanoyl spacer (known as X ) between TAMRA fluorophore and the succinimidyl ester. The X spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. We recommend this TAMRA-X derivative as the preferred dye for preparing TAMRA-labeled proteins when the fluorescence quenching of the labeling dye by protein is a serious problem.; C35H36N4O8
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VivoTag-S 750 amine-reactive near-infrared fluorochrome for coupling via an NHS ester linkage to peptides, small molecules, proteins, antibodies or macromolecules. 5 mg quantity.
In proteins and peptides, d-aspartic acid (d-Asp) and d-β-Asp residues can be spontaneously formed via racemization of the succinimide intermediate formed from l-Asp and l-asparagine (l-Asn) residues. These biologically uncommon amino acid residues are known to have relevance to aging and pathologies. Although nonenzymatic, the succinimide racemization will not occur without a catalyst at room or biological temperature. In the present study, we computationally investigated the mechanism of succinimide racemization catalyzed by dihydrogen phosphate ion, H2PO4−, by B3LYP/6-31+G(d,p) density functional theory calculations, using a model compound in which an aminosuccinyl (Asu) residue is capped with acetyl (Ace) and NCH3 (Nme) groups on the N- and C-termini, respectively (Ace-Asu-Nme). It was shown that an H2PO4− ion can catalyze the enolization of the Hα-Cα-C=O portion of the Asu residue by acting as a proton-transfer mediator. The resulting complex between the enol form and H2PO4− corresponds to
Haemopexin receptors from mouse hepatoma (Hepa) cells were affinity-labelled by cross-linking to haem-125I-haemopexin complexes using two homo-[disuccinimidyl suberate (DSS) and 3,3′-dithiobis(succinimidyl propionate) (DTSSP)] and one hetero-[sulphosuccinimidyl 4-(p-maleimidophenyl)butyrate (sulpho-SMPB)] bifunctional cross-linking agents. Analysis of the cross-linked products by SDS/PAGE in the absence of reducing agents revealed that 125I-haemopexin was cross-linked specifically to a protein of apparent molecular mass 85-90 kDa. Upon reduction, haemopexin remained cross-linked to a protein of 20 kDa, suggesting that the murine haemopexin receptor has a subunit structure. Two subunits were identified: alpha (p65) and beta (p20). Furthermore, because haemopexin was cross-linked by all three agents to p20, the shortest cross-linker arm being 1.1 nm (11 A), we propose that the haem-haemopexin-binding site resides on this subunit. In addition, a cysteine residue of p20 is located near the ...
Background: Sublingual immunotherapy (SLIT) efficacy could be improved by formulations facilitating allergen contact with the oral mucosa and uptake by antigen-presenting cells (APCs).. Methods: Two types of chitosan microparticles, differing in size and surface charge, were tested in vitro for their capacity to improve antigen uptake and presentation by murine bone marrow-derived dendritic cells (BMDCs) or purified oral APCs. T-cell priming in cervical lymph nodes (LNs) was assessed by intravenous transfer of carboxyfluorescein diacetate succinimidyl ester-labelled ovalbumin (OVA)-specific CD4+ T cells and flow cytometry analysis. Ovalbumin-sensitized BALB/c mice were treated sublingually with soluble or chitosan-formulated OVA twice a week for 2 months. Airway hyperresponsiveness (AHR), lung inflammation and T-cell responses in cervical and mediastinal LNs were assessed by whole-body plethysmography, lung histology and Cytometric Bead Array technology, respectively.. Results: Only a ...
Rivotril belongs to a class of drugs known as benzodiazepines .Rivotril is used alone or along with other medications to treat convulsive disorders such as epilepsy. Clonazepam is metabolized by the liver to inactive metabolites, which are excreted mainly in the urine. It is also prescribed for panic disorder--unexpected attacks of overwhelming panic accompanied by fear of recurrence.. I n this multicenter, parallel-group, placebo-controlled, fixed-dose study, the efficacy, safety, dosing characteristics, and discontinuation of clonazepam were analyzed in patients with panic disorder.. Rivotril is useful alone or as an adjunct in the treatment of the Lennox-Gastaut syndrome (petit mal variant), akinetic and myoclonic seizures. In patients with absence seizures (petit mal) who have failed to respond to succinimides, Rivotril may be useful. ...
Treatment of quiescent cultures of mouse embryo fibroblasts with 20% fetal calf serum (FCS) or cycloheximide (CH) resulted in the induction of a nuclear protein of molecular weight 29 000 daltons. The 29 Kd protein induced by these two agents was found to be tightly bound to the chromatin since it was not released from the chromatin by a combination of 2.5 M NaCl and 3.2 M urea. The chromatin associated CH-induced 29 Kd protein and the 29 Kd protein obtained from serum-induced cells displayed similar N-chlorosuccinimide cleavage patterns. Pulse-chase experiments indicate a half-life of an hour for the 29 Kd protein. The kinetics of induction of the 29 Kd protein in the early hours of mitogen addition, short half-life, nuclear localisation and strong association with chromatin suggest that this protein may have important roles in cell proliferation, possibly as a mediator of mitogen action. ...
This invention is to a fuel oil composition containing polyolefinic succinimide polyamine alkyl acetoacetate adducts of the general following: ##STR1## wherein the variables are defined herein. The adduct additives are especially useful in concentrates and fuel oil compositions as dispersants. The formulations have excellent cold flow properties compared to similar formulations not containing the inventive adduct.
... are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, N2-Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labeled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide. O ur repertoire of NHS ester derivatives has been expanded to include the NHS-Carboxy-dT-CE Phosphoramidite. By making a dT analog of the ...
4] a ) J. T. Kauer, S. Erickson-Vitanen, H. R. Wolfe, W. F. De Grddo, J B d . Chrm. 1986.261. 10695, b) W. T. Miller, E. T. Kaiser. Proc. Nurl. Arud. Sci. U S A 1988, K S , 5429. c) T. Tao. C. J. Schemer, M. Lamkin. 5io~liemistr.v 1986.2.7. 7633. [5] a ) R. Breslow, Acr. Chrm. Res. 1980, 13, 170, b) C . Helene, Phofochem. Pholohiol. 1972, 16. 519. [6] G. W. Anderson, J. E. Zimmerman. F. M. Gallahan, J. Am. Cheni. Sor. 1964. K6. 1839. [7] A section of a silica substrate bearing nitroveratryloxycarbonyl(NV0C)protected amino groups was photodeprotected [3] and then derivatized uith the NHS ester of 3-benzoylbenzoic acid. A second section of the substrate was then deprotected and derivatized with the NHS ester of biotin. Any unreacted sites in the first section (due to incomplete coupling of benzophenone) will also react with the biotin NHS ester. The entire surface was then treated with fluorescein-streptavidin (Molecular Probes lnc.. Eugene. O R (USA)). The coupling efficiency of benzophenone was ...
Fluorescent labelling of monoclonal antibodies (mAbs) is classically performed by chemical bioconjugation methods. The most frequent labelling technique to generate antibody-fluorophore conjugates (AFCs) involves the bioconjugation onto the mAb lysines of a dye bearing an N-hydroxysuccinimide ester or an isothiocyanate group. However, discrepancies between labelling experiments or kits can be observed, related to reproducibility issues, alteration of antigen binding, or mAb properties. The lack of information on labelling kits and the incomplete characterization of the obtained labelled mAbs largely contribute to these issues. In this work, we generated eight AFCs through either lysine or interchain cysteine cross-linking bioconjugation of green-emitting fluorophores (fluorescein or BODIPY) onto either trastuzumab or rituximab. This strategy allowed us to study the influence of fluorophore solubility, bioconjugation technology, and antibody nature on two known labelling procedures. The structures of
Beyond providing Skin Deep® as an educational tool for consumers, EWG offers its EWG VERIFIED™ mark as a quick and easily identifiable way of conveying personal care products that meet EWGs strict health criteria. Before a company can use EWG VERIFIEDTM on such products, the company must show that it fully discloses the products ingredients on their labels or packaging, they do not contain EWG ingredients of concern, and are made with good manufacturing practices, among other criteria. Note that EWG receives licensing fees from all EWG VERIFIED member companies that help to support the important work we do. Learn more , Legal Disclaimer ...
AnaTag Protein Labeling Kits , Classic Fluorescent Dye Labeling Kits , AnaTag AMCA - X Microscale Protein Labeling Kit *Ultra Convenient*; AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group. This spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. AMCA fluorescence can be detected at the emission wavelength of 442 10 nm when excited at 353 10 nm. The AMCA-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the AMCA-protein conjugates. The kit is able to perform three conjugation reactions with each reaction
AnaTag™ HiLyte Fluor™ Protein Labeling kits provide a way to label proteins using the succinimidyl ester reactive form of the HiLyte Fluor™ dyes.
Surfaces with primary or secondary amino groups covalently bound are dedicated to promote the covalent immobilisationof compounds containing reactive moieties such as amino, carboxyl or thiol groups via well-known homoheterobifunctionallinkers, e.g. N-Hydroxysuccinimide (NHS) or Succinimidyl 4-(N-maleidomethyl) cyclohexane-1-carboxylate (SMCC).. This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules tothe surfaces:. ...
Surfaces with primary or secondary amino groups covalently bound are dedicated to promote the covalent immobilisationof compounds containing reactive moieties such as amino, carboxyl or thiol groups via well-known homoheterobifunctionallinkers, e.g. N-Hydroxysuccinimide (NHS) or Succinimidyl 4-(N-maleidomethyl) cyclohexane-1-carboxylate (SMCC).. This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules tothe surfaces:. ...
Preparation of morphine-N-(ε-trifluoroacetylcaproyloxy) succinimide ester (TFCS)-keyhole limpet hemocyanin (KLH), showing conjugation of 6-glutarylmorphine (M-
Find more than 1700 high purity PEG linkers with a broad selection of funcitonal groups (amine, azide, maleimide, NHS ester, bromide, etc) to inspire your PEGylation. Order now!
Reactive PromoFluor dyes (available as e.g. NHS esters or maleimides) for labeling of biomolecules such as proteins, antibodies and nucleic acids.
Page contains details about carboxyfluorescein loaded bolaamphiphilic nanovesicle . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Highly hydrophilic, amine-reactive label containing one NHS-ester group. A brighter and more photostable replacement for Alexa 633, with a much higher extinction coefficient (250,000) and brightness after labeling to proteins. ...
The Spectrolinker XL-1000 UV crosslinker was made for applications including eliminating PCR contamination, cross-linking DNA an...
When a low concentrations of crosslinker is added to resist (a film used to lay down the patterns of ever-shrinking lines and features on a chip, shown here at left), it is able to pattern smaller ...
Used in the treatment of epilepsy. Ethosuximide suppresses the paroxysmal three cycle per second spike and wave activity associated with lapses of consciousness which is common in absence (petit mal) seizures. The frequency of epileptiform attacks is reduced, apparently by depression of the motor cortex and elevation of the threshold of the central nervous system to convulsive stimuli ...
A mild procedure for the reduction of electron-deficient alkenes and carbonyl compounds is described. UVA irradiations of substituted maleimides with dispersions of titania (Aeroxide P25) in methanol/acetonitrile (1:9) solvent under dry anoxic conditions led to hydrogenation and production of the corresponding succinimides. Aromatic and heteroaromatic aldehydes were reduced to primary alcohols in similar titania photocatalyzed reactions. A mechanism is proposed which involves two proton-coupled electron transfers to the substrates at the titania surface.
Purpose: Choroidal neovascularization (CNV) is a commonly occuring pathology in various eye diseases, e.g. in an advanced stage of age-related macular degeneration (AMD). Vascular endothelial growth factor (VEGF) is known to be upregulated and leads to enhanced endothelial cell growth. The polyphenols resveratrol, epigallocatechine gallate (EGCG) and curcumin have antiproliferative and antiinflammatory effects. In this study, the effects of these polyphenols on human retinal endothelial cells (hREC) were investigated in a cell culture model.. Methods: hREC were cultured in 2% serum-containing cell culture medium and different concentrations of resveratrol, EGCG and curcumin were tested. Flow-cytometric analysis was performed after 24, 48 and 72 hours of incubation. Absolute cell numbers were counted, cell proliferation was measured with the Carboxyfluorescein succinimidyl ester (CFSE) dilution assay and dead cells were analysed with the nuclear dye Hoechst 33258. Apoptotic cells could be ...
0044]The (4-methyl-thiophen-3-yl)-carbamic acid tert-butyl ester was then selectively chlorinated with a chlorinating agent such as N-chlorosuccinimide (NCS) and catalytic hydrochloric acid (HCl) or alternatively, by metalation with N-butyl lithium and reaction with hexachloroethane (Cl3C2Cl3) to form 2-chloro-4-methyl-thiophen-3-yl)-carbamic acid tert-butyl ester. This ester is then treated with hydrogen chloride gas to remove the protecting group so as to form the 3-amino-2-chloro-4-methylthiophene hydrochloride. In step 3, the 3-amino-2-chloro-4-methylthiophene hydrochloride is activated for coupling by treatment with phenyl chlorothionoformate and sodium bicarbonate. This intermediate was subsequently treated with 1,2-phenylenediamine and triethylamine to afford N-(2-aminophenyl)-N-(2-chloro-4-methyl-3-thienyl)thiourea. The thiourea was cyclized utilizing an alkyl or arylsulfonyl chloride such as p-toluenesulfonyl chloride or benzenesulfonyl chloride together with an alkali metal base such ...
ChemicalBook あなたのためにPYRROLIDINE-2-CARBOXYLIC ACID (4-METHOXY-PHENYL)-AMIDE(1163686-79-0)の化学的性質を提供して、融点、価格、蒸気圧、沸点、毒性、比重、沸点、密度、分子式、分子量、物理的な性質、毒性 税関のコードなどの情報、同時にあなたは更にPYRROLIDINE-2-CARBOXYLIC ACID (4-METHOXY-PHENYL)-AMIDE(1163686-79-0)の製品の全世界の供給商にブラウズすることができて、生産企業と生産メーカー、最後のPYRROLIDINE-2-CARBOXYLIC ACID (4-METHOXY-PHENYL)-AMIDE(1163686-79-0)の中国語、英文、用途 CAS cas number cas no可能性もあなたが必要とします。
An Electrochemical DNA Microbiosensor Based on Succinimide-Modified Acrylic Microspheres. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Amine Reactive PEG Derivatives and PEGylation Reagents: PEG NHS ester - N-hydroxyl succinimidyl ester (SCM, glutarate, succinate, glutaramide) PEG aldehyde - PEG propionaldehyde PEG carboxylic acid - carboxyl methyl, acetic acid, glutaric and succinic acid PEG cyanur, bromide, and chloride PEG mesylate and tosylate PEG NPC - nitrophenyl carbonate PEG epoxide - PEG glycidyl ether
TY - JOUR. T1 - Basic carboxyl groups of hemoglobin S. T2 - Influence of oxy-deoxy conformation on the chemical reactivity of Glu-43(β). AU - Rao, M. Janardhan. AU - Acharya, A. Seetharama. PY - 1991/2/1. Y1 - 1991/2/1. N2 - The γ-carboxyl groups of Glu-43(β) and Glu-22(β) of hemoglobin-S (HbS), two intermolecular contact residues of deoxy protein, are activated by carbodiimide at p H 6.0. The selectivity of the modification by the two nucleophiles, glycine ethyl ester (GEE) and glucosamine, is distinct. Influence of N-hydroxysulfosuccinimide, a reagent that rescues carbodiimide-activated carboxyl (O-acyl isourea) as sulfo-NHS ester, on the overall selectivity and efficiency of the coupling of Glu-22(β) and Glu-43(β) with nucleophiles has been investigated. Sulfo-NHS increases the extent of coupling of nucleophiles to HbS. The rescuing efficiency of sulfo-NHS(increase in modification) with GEE and galactosamine as nucleophiles is 2.0 and 2.8, respectively. In the presence of sulfo-NHS, the ...
en] Functionalized poly-ε-caprolactone-block-polyethyleneglycol (PCL-PEG) amphiphilic copolymers were prepared to be constituents of nanocarriers used for the targeting of specific cells. Hence, we conceived a smooth and simple photografting methodology on these copolymers using a bifunctional molecular clip (O-succinimidyl-4-(p-azido-phenyl)butanoate). We prepared PCL-PEGs with pendent N-hydroxysuccinimide esters and studied the grafting with 3H-lysine, which radioactivity was counted by LSC. Several parameters were investigated, such as behavior of homopolymers, initial concentrations, irradiation, and incubation durations. Evidences of a "PEG directed photografting" are discussed and this selectivity could be improved by a selective solvent technique. The photografting on different PCL-PEGs revealed a dependency of the rates to the crystallinity of the copolymers. Several controls by SEC, DLS, and TEM of the treated copolymers were realized. Lastly, the coupling of α-d-mannopyranoside ...
The Gal1,3GalNAc1,O-Ser/Thr particular lectin from (improved cell proliferation just like those cells activated via CD3/CD28 at 48?h of tradition. well mainly because soluble and intracellular cytokines, as well as the incomplete characterization of the primary lipid raft glycoprotein identified by seed products were acquired in Tulyehualco (Mexico) as well as the lectin was purified simply by affinity chromatography mainly because referred to previously 9. was tagged using the N-hydroxysuccinimide ester of biotin from Pierce Chemical substance (Rockford, IL) having a label/proteins percentage of 2:1 16. Phycoerythrin (PE)-tagged rat anti-mouse Compact disc4, biotin-labeled hamster anti-mouse Compact disc3? string YK 4-279 (145-2C11) monoclonal antibodies (mAbs), and PE-labeled rat IgG2a, kappa mAb (utilized as isotype control); purified no azide/low endotoxin (NA/LE) hamster anti-CD3 (clone 145-2C11) or anti-CD28 (clone 37.51) mAbs (utilized to activate T cells); PE-cyanine (Cy) 5-, fluorescein ...
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(2S,5R)-5-(4-Aminophenyl)-1-[2-[[(2S)-3-(4-hydroxyphenyl)-2-sulfanylpropanoyl]amino]acetyl]pyrrolidine-2-carboxylic acid | C22H25N3O5S | CID 44322098 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
1AT5: Succinimide and isoaspartate residues in the crystal structures of hen egg-white lysozyme complexed with tri-N-acetylchitotriose.
Fig. 1 , Molecular architecture of Pol II(G) by cryo-eM and CX-MS analyses. a, Cryo-EM structure of Pol II(G). Non-Pol II (Gdown1) density is shown in purple. An atomic model of human Pol II was obtained by real-space refinement of a model derived from relevant portions of the published model of bovine Pol II (PDB 5FLM). To identify Gdown1 density in the Pol II(G) map, the difference between all stable portions of the Pol II(G) cryo-EM map was calculated. b, Diagram of cross-links between Pol II subunits and Gdown1. Purified Pol II(G) was subjected to cross-linking with the amine-specific cross- linker, disuccinimidyl suberate (DSS), followed by high-resolution MS. c, Intra-molecular cross-links map of Gdown1. (from Jishage et al (2018). Nat Stuct Mol Biol). ...
NEX313 Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor κB (NFκB). ...
It is important to tell your doctor if you become pregnant while using this medicine. Your doctor may want you to join a pregnancy registry for patients taking a seizure medicine. For some children, teenagers, and young adults, this medicine can increase thoughts of suicide. Tell your doctor or your childs doctor right away if you or your child start to feel more depressed or have thoughts about hurting yourself. Report any unusual thoughts or behaviors that trouble you or your child, especially if they are new or get worse quickly. Make sure the doctor knows if you or your child have trouble sleeping, get upset easily, have a big increase in energy, or start to act reckless. Also tell the doctor if you or your child have sudden or strong feelings, such as feeling nervous, angry, restless, violent, or scared. Let the doctor know if you, your child, or anyone in your family has bipolar disorder (manic-depressive) or has tried to commit suicide. Do not stop taking this medicine without first ...
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Amine functionality is indigenous to an antibody and can be used for conjugation to lipids containing various reactive functional groups such as activated carboxylic acid or NHS ester to form a stable amide linkage or cyanuryl chloride forming a stable amine linkage.. Conjugation through N Terminus. ...
diflorasone diacetate 33564-31-7 NMR spectrum, diflorasone diacetate H-NMR spectral analysis, diflorasone diacetate C-NMR spectral analysis ect.
Hi... Im seriously considering selling my KEF eggs on these forums. I understand that I need to specify an asking price. Id be grateful if anyone...
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe. Info: HEX; Same primer pair and probe as used in qPCR assay qRnoCEP0043632; exonic ...
The preparation of a new poly(thiophene) with pendant N-hydroxysuccinimide ester groups and its application to immobilization of biomolecules are reported. A thiophene derivative of N-hydroxysuccinimide ester was polymerized with FeCl3 in chloroform and the resulting poly(thiophene) was characterized by nuclear magnetic resonance (NMR), Fourier transform infrared (FT-IR), and gel permeation chromatography (GPC). This polymer reacts with amine-bearing molecules to yield new poly(thiophene) derivatives and the specific interactions at the side groups could be detected. Thus, a self-assembled monolayer (SAM) using the polymer was formed on a gold-coated quartz cell and anti-C-reactive protein (anti-CRP) was immobilized. The binding behavior of CRP on the surface was monitored by use of a surface plasmon resonance (SPR) sensor system. © 2008 Elsevier B.V. All rights reserved. ...
A radiolabeled human neutrophil elastase inhibitor (EPI-HNE-2) may represent an improved nuclear medicine imaging agent for inflammation and infection. This peptide displays rapid pharmacokinetics due to its low molecular weight and localizes specifically on neutrophil elastase released in inflammatory sites by activated neutrophils. METHODS: In this investigation, the peptide was radiolabeled with 99mTc using N-hydroxysuccinimidyl S-acetylmercaptoacetyltriglycline (NHS-MAG3) as a bifunctional chelator and was administered on 18 occasions to 5 rhesus monkeys with inflammation/infection. RESULTS: Plasma clearance was rapid, with liver and kidneys representing the major organs of accumulation. No evidence of toxicity, dosage effects, or circulating antiMAG3-EPI-HNE-2 antibodies was observed. Specificity of localization was established using radiolabeled bovine pancreatic trypsin inhibitor (a non-hNE-binding peptide of similar size) as a nonspecific negative control peptide and by predosing with unlabeled
MAXY-G34 is a pegylated variant of human granulocyte-colony stimulating factor (G-CSF). This variant contains multiple non-naturally occurring lysines that have been introduced into alpha helixes of wild type human G-CSF as PEGylation sites, and from which multiple undesired, naturally occurring lysines have been removed as compared to wild type human G-CSF to avoid PEGylation of such sites. Specifically, the amino acid sequence of MAXY-G34 differs from that of human wild type G-CSF at residues 16, 34, 40, 105 and 159. This was accomplished by removing the three lysine residues at positions 16, 34 and 40, and replacing them with arginine, and substituting two new lysine residues at positions 105 and 159. MAXY-G34 is pegylated with 5 kd mPEG SPA (succinimidyl propionate) groups at 3 amino acid residues, including PEG groups attached at the amino terminal end of the protein and at two internal lysine residues, while Neulasta has a single 20 Kd PEG group attached at the N terminal end of the wild type G
Sigma-Aldrich offers bifunctional and trifunctional linkers and crosslinking reagents for various conjugation applications.

There are numerous bioconjugation possibilities with our linkers and crosslinkers, offering potential for structural stability or assistance in protein-protein, protein-peptide and peptide/protein-small molecule interactions. Other applications include immobilization for assays or purification, as well as various peptide-nucleic acid and nucleic acid-nucleic acid conjugations, among many others.

Our homo- and heterofunctional linkers contain diverse functional groups, such as primary amines, sulfhydryls, acids, alcohols and bromides. Many of our crosslinkers are functionalized with maleimide (sulfhydral reactive) and succinimidyl ester (NHS) or isothiocyanate (ITC) groups that react with amines. A selection of mono-protected (Boc, Fmoc, and Cbz) linkers is also available.
Visit ChemicalBook To find more N-Bromosuccinimide(128-08-5) information like chemical properties,Structure,melting point,boiling point,density,molecular formula,molecular weight, physical properties,toxicity information,customs codes. You can also browse global suppliers,vendor,prices,Price,manufacturers of N-Bromosuccinimide(128-08-5). At last,N-Bromosuccinimide(128-08-5) safety, risk, hazard and MSDS, CAS,cas number,Use,cas no may also be you need.
EX/Em(nm): 649nm/664nm. MW: 1050.35 Da. Solvent: DMSO. Storage: Freeze(,-15 degree), Desiccated, Avoid Light. iFluor 647 SE are superior in photo-stability and the dye can be well adapted to solution/buffer which has pH ranged from 3-11. iFluor 647 SE has spectral properties similar to Alexa Fluor 647 NHS ester and Cy5 (Alexa Fluor is the trademark of Invitrogen, Cy5 is the trademark of GE healthcare). iFluor 647 SE has similar reactive mechanism as Alexa Fluor 647 NHS ester. ...
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin.
Prenotochordal cellsinvaginating in the primitive pit move forward cephalad until they reach theprechordal plate. These prenotochordal cells become intercalated in the hypoblast so that, for a short time, the midline of the embryo consists of two cell layers that form the notochordal plate. As the hypoblast is replaced by endodermcells moving in at the streak,cells of the notochordal plate proliferate and detach from the endoderm. They then form a solid cord of cells, the definitive notochord,which underlies the neural tube and serves as the basis for the axial skeleton.Because elongation of the notochord is a dynamic process, the cranial end forms first, and caudal regions are added as the primitive streak assumes a more caudal position. The notochord and prenotochordal cells extend cranially to the prechordal plate (an area just caudal to the buccopharyngeal membrane)and caudally to the primitive pit. At the point where the pit forms an indentation in the epiblast, the neurenteric canal ...
Prenotochordal cellsinvaginating in the primitive pit move forward cephalad until they reach theprechordal plate. These prenotochordal cells become intercalated in the hypoblast so that, for a short time, the midline of the embryo consists of two cell layers that form the notochordal plate. As the hypoblast is replaced by endodermcells moving in at the streak,cells of the notochordal plate proliferate and detach from the endoderm. They then form a solid cord of cells, the definitive notochord,which underlies the neural tube and serves as the basis for the axial skeleton.Because elongation of the notochord is a dynamic process, the cranial end forms first, and caudal regions are added as the primitive streak assumes a more caudal position. The notochord and prenotochordal cells extend cranially to the prechordal plate (an area just caudal to the buccopharyngeal membrane)and caudally to the primitive pit. At the point where the pit forms an indentation in the epiblast, the neurenteric canal ...
In this work, intramolecular and intermolecular associations under the effect of shear flow of dilute aqueous alkaline solutions of dextan, hydroxyethylcellulose (HEC), and the hydrophobically modified analogue (HM-2-HEC) in the presence of a chemical cross-linking agent were characterized with the aid of viscometry and rheo-small angle light scattering (rheo-SALS) methods. The picture that emerges at short times during the cross-linking reaction at a constant shear rate is that HEC coils contract because of intramolecular cross-linking; whereas the HM-2-HEC species show an incipient association and the dextran molecules are unaffected due to their compact structures. At longer times, interchain cross-linking of the polymer promotes the growth of large flocs, which are disrupted by shear forces when they are sufficiently large. The delicate interplay between intramolecular and intermolecular association is governed by factors such as the magnitude of the shear rate, the cross-linking agent ...
Founded in 2011, Suzhou Xinkai Bio-Medical Technology Co., Ltd. is located in Suzhou New District of Suzhou city in Jiangsu province. Xinkai focus is on the development of...
1. Birner, G., et al., Metabolism of tetrachloroethene in rats: identification of N epsilon-(dichloroacetyl)-L-lysine and N epsilon-(trichloroacetyl)-L-lysine as protein adducts. Chem Res Toxicol, 1994. 7(6): p. 724-32.. 2. Pahler, A., et al., Generation of antibodies to Di- and trichloroacetylated proteins and immunochemical detection of protein adducts in rats treated with perchloroethene. Chem Res Toxicol, 1998. 11(9): p. 995-1004.. 3. Kleiner, H.E., et al., Immunochemical detection of quinol--thioether-derived protein adducts. Chem Res Toxicol, 1998. 11(11): p. 1283-90.. 4. Lau, S.S., Quinone-thioether-mediated nephrotoxicity. Drug Metab Rev, 1995. 27(1-2): p. 125-41.. 5. Tune, B.M., Nephrotoxicity of beta-lactam antibiotics: mechanisms and strategies for prevention. Pediatr Nephrol, 1997. 11(6): p. 768-72.. 6. Griffin, R.J. and P.J. Harvison, In vivo metabolism and disposition of the nephrotoxicant N-(3, 5-dichlorophenyl)succinimide in Fischer 344 rats. Drug Metab Dispos, 1998. 26(9): p. ...
PRODUCTO ETX Cross-linking agent that improves attachment and wash fastness of resins. PRODUCTO R3 conc. Self-cross-linking agent of resin that improves wash and rubbing fastness of pigments.
สาริกานนท์, จำลอง; สนธิสมบัติ, อภิชาติ (Rajamangala University of Technology Phra Nakhon, 2009-03-01) ...
Learn about ApexiCon E (Diflorasone Diacetate) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and related medications.
As for the Belly Laugh portion of my post... Getting Malakai to laugh is like trying to pull teeth from a chicken (no laughing matter, I assure you!). I know he can laugh, I have heard it! Although it has never been a giggle, he has chuckled (like choking on a cough...) for me before! So today I discovered something that he thought was hilarious - and by the picture above you can see that! But no sound... Just a little choke noise once or twice. He IS laughing, but without sound? But just like everything else in my little mans life - he will do what he does in his time - he knows best after all ...
0101] The alcoholic compound of formula V is converted to a suitable halide or sulfonate ester of formula V-A by treatment with a suitable reagent in a suitable solvent. A non-limiting list of suitable halides and sulfonate esters includes chlorides, bromides, iodides, and alkyl- and arylsulfonates including methanesulfonate, toluenesulfonate and nitrobenzenesulfonate. A non-limiting list of suitable reagents includes thionyl chloride, oxalyl chloride, phosphorus oxychloride, phosphorus trichloride, phosphorus pentachloride, hydrogen chloride, acetyl chloride, hydrogen bromide, phosphorus tribromide, phosphorus pentabromide, thionyl bromide, boron tribromide, trimethylsilyl iodide, triphenylphosphine-chlorine complex, triphenylphosphine and N-chlorosuccinimide, triphenylphosphine and bromine, triphenylphosphine and N-bromosuccinimide, triphenylphosphine and iodine, triphenylphosphine and N-iodosuccinimide, triphenylphosphine and carbon tetrachloride, triphenylphosphine and carbon tetrabromide, ...
A method of making a gel drop protein chip by transferring proteins, which were obtained from a cellular lysate, partitioned using two-dimensional, protein fractionation, and mixed with a polymeric matrix solution containing acrylamide/bis and glycerol, to an array; a method of making a gel drop protein chip by transferring proteins, which were derivatized with N-hydroxysuccinimide ester of N-methacryloyl-6-aminocaproic acid (NHS monomer), and mixed with a polymeric matrix solution containing acrylamide/bis and glycerol, to an array; a gel drop protein chip containing proteins in a polymeric matrix solution containing acrylamide/bis glycerol; a method of using the gel drop protein chip to interrogate a sample; and a protein derivatized with the NHS monomer.
Mice. Mice were housed in a humidity-controlled facility and kept on a 12-hour light/dark cycle. Male C57BL/6J mice (Jackson Laboratory) aged 11-13 weeks fed a standard chow diet (5001 Laboratory Rodent) were used for all experiments. Mice were fasted for 5 hours before all imaging and metabolic phenotyping experiments.. INS-647 synthesis and analysis. The conjugation of Alexa Fluor 647 to insulin follows a modified version of a procedure described previously (39). Diisopropylethylamine (DIPEA; 7 mmol, 1.22 ml, 40 Eq) was initially added to a solution of biosynthetic human insulin (BHI; 0.2 mmol, 1.16 g) dissolved in DMSO (15 ml). (Butyloxycarbonyl)succinimide ester (Boc-OSu; 0.445 mmol, 95.7 mg, 2.5 Eq) was then dissolved in DMSO (2 ml) and added slowly to the BHI solution over 5 minutes. After 40 minutes, the reaction was quenched with trifluoroacetic acid (TFA; 100 μl) and diluted with 0.1 N HCl (150 ml). The reaction mixture was purified by reverse-phase HPLC (RP-HPLC; Waters 19 × 300 mm ...
Mice. Mice were housed in a humidity-controlled facility and kept on a 12-hour light/dark cycle. Male C57BL/6J mice (Jackson Laboratory) aged 11-13 weeks fed a standard chow diet (5001 Laboratory Rodent) were used for all experiments. Mice were fasted for 5 hours before all imaging and metabolic phenotyping experiments.. INS-647 synthesis and analysis. The conjugation of Alexa Fluor 647 to insulin follows a modified version of a procedure described previously (39). Diisopropylethylamine (DIPEA; 7 mmol, 1.22 ml, 40 Eq) was initially added to a solution of biosynthetic human insulin (BHI; 0.2 mmol, 1.16 g) dissolved in DMSO (15 ml). (Butyloxycarbonyl)succinimide ester (Boc-OSu; 0.445 mmol, 95.7 mg, 2.5 Eq) was then dissolved in DMSO (2 ml) and added slowly to the BHI solution over 5 minutes. After 40 minutes, the reaction was quenched with trifluoroacetic acid (TFA; 100 μl) and diluted with 0.1 N HCl (150 ml). The reaction mixture was purified by reverse-phase HPLC (RP-HPLC; Waters 19 × 300 mm ...
Mice. Mice were housed in a humidity-controlled facility and kept on a 12-hour light/dark cycle. Male C57BL/6J mice (Jackson Laboratory) aged 11-13 weeks fed a standard chow diet (5001 Laboratory Rodent) were used for all experiments. Mice were fasted for 5 hours before all imaging and metabolic phenotyping experiments.. INS-647 synthesis and analysis. The conjugation of Alexa Fluor 647 to insulin follows a modified version of a procedure described previously (39). Diisopropylethylamine (DIPEA; 7 mmol, 1.22 ml, 40 Eq) was initially added to a solution of biosynthetic human insulin (BHI; 0.2 mmol, 1.16 g) dissolved in DMSO (15 ml). (Butyloxycarbonyl)succinimide ester (Boc-OSu; 0.445 mmol, 95.7 mg, 2.5 Eq) was then dissolved in DMSO (2 ml) and added slowly to the BHI solution over 5 minutes. After 40 minutes, the reaction was quenched with trifluoroacetic acid (TFA; 100 μl) and diluted with 0.1 N HCl (150 ml). The reaction mixture was purified by reverse-phase HPLC (RP-HPLC; Waters 19 × 300 mm ...
Gear oils and gear oil additive concentrates of enhanced positraction performance are described. They comprise: (i) at least one oil-soluble sulfur-containing extreme pressure or antiwear agent; (ii) at least one oil-soluble amine salt of a partial ester of an acid of phosphorus; and (iii) at least one oil-soluble succinimide of the formula ##STR1## where R.sub.1 is an alkyl or alkenyl group having an average of 8 to 50 carbon atoms, and each of R.sub.2, R.sub.3 and R.sub.4 is a hydrogen atom or an alkyl or alkenyl group having an average of up to about 4 carbon atoms. These compositions preferably contain one, more preferably two, and most preferably all three of the following additional components: (iv) at least one amine salt of a carboxylic acid; (v) at least one nitrogen-containing ashless dispersant; and (vi) at least one trihydrocarbyl ester of a pentavalent acid of phosphorus.
An accurate dye dilution testing protocol using Rhodamine WT was developed and used to quantify flow meter accuracy in the Greater Detroit Regional Sewer Syste…
Amino dA C6 can be used to internally incorporate an active primary amino group into either a DNA oligonucleotide or a chimeric oligo. The presence of the primary amino group allows the user to label the oligo with a variety of different ligands for affinity, reporter or protein moieties (as NHS esters or isothiocyanates), depending on the application. Examples include biotin, digoxigenin, and fluorescent dyes or quenchers, magnetic beads and enzymes (for example, alkaline phosphatase).. ...
Amino dG C6 can be used to internally incorporate an active primary amino group into either a DNA oligonucleotide or a chimeric oligo. The presence of the primary amino group allows the user to label the oligo with a variety of different ligands for affinity, reporter or protein moieties (as NHS esters or isothiocyanates), depending on the application. Examples include biotin, digoxigenin, and fluorescent dyes or quenchers, magnetic beads and enzymes (for example, alkaline phosphatase).. ...
With regards to conjugation to KLH, Globo-H, MUC1-32mer, GM2, Lewis Y, Tn(c), STn(c), and TF(c) were covalently attached to KLH directly by reductive amination (GM2), using the 4-(4-maleimidomethyl) cyclohexane-1-carboxyl hydrazide heterobifunctional linker group (Globo-H and Lewis Y) and using the m-malemidobenzoyl-N-hydroxysuccinimide ester heterobifunctional linker group [MUC1, Tn(c), STn (c), and TF (c)] as described previously (18, 20-25, 29). The structures of these seven conjugates are summarized in Fig. 1. Antigen and adjuvant doses were selected based on previous phase 1 monovalent vaccine trials as follows: 3 μg Tn-MUC1-32mer (24), 10 μg Globo-H (15, 22), 10 μg GM2 (30), 10 μg Lewis Y (20), 3 μg Tn(c) (23), 3 μg STn(c), 3 μg TF(c) (18), and 100 μg QS21 (15, 30). These seven conjugates and QS21 were vialed together in 1 mL PBS. Vialed samples underwent testing for toxicity and immunogenicity in mice, as well as for sterility and endotoxin. Vaccines were given s.c. on weeks 1, 2, ...
Polyamides comprising substantial amounts of Nylon-11 and/or Nylon-12 units are cross-linked by irradiation in the presence of an unsaturated cross-linking agent, preferably triallyl isocyanurate. The cross-linked products are particularly useful in the form of heat-recoverable shaped articles, e.g. heat-shrinkable tubing.
I do a proliferation assay after pulsing PBMCS with CFSE (5micromol for 2-10million cells) and then staining with CD3. I sort the cells and then stimulate them for proliferation with PHA(2microlitre). I also stain the cells with CD4 and CD8 on the day of analysis. I add PI jus before running the cells on the flow cytometer to discriminate for live vs dead cells.I am worried because I only get a single peak for each day (1-5 days expt). am i using too much CFSE? is that making it difficult to discern the peaks? Can somebody help with the trouble shooting. I also apply single compensation controls for the first day and find no spill for the rest of the days by using the same settings ...
I do a proliferation assay after pulsing PBMCS with CFSE (5micromol for 2-10million cells) and then staining with CD3. I sort the cells and then stimulate them for proliferation with PHA(2microlitre). I also stain the cells with CD4 and CD8 on the day of analysis. I add PI jus before running the cells on the flow cytometer to discriminate for live vs dead cells.I am worried because I only get a single peak for each day (1-5 days expt). am i using too much CFSE? is that making it difficult to discern the peaks? Can somebody help with the trouble shooting. I also apply single compensation controls for the first day and find no spill for the rest of the days by using the same settings ...
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[102 Pages Report] Check for Discount on Global Glycerol Diacetate Sales Market Report 2017 report by QYResearch Group. In this report, the global Glycerol Diacetate market is valued...
Some aspects of this disclosure relate to a method for crosslinking a biological fluid comprising combining a biological fluid with a crosslinker to covalently crosslink proteins endogenous to the biological fluid to form a crosslinked gel. Examples of a biological fluid are blood, plasma, or serum.