Cytomegalovirus-induced embryopathology: mouse submandibular salivary gland epithelial-mesenchymal ontogeny as a model. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Background: The purpose of the present study was to elucidate whether myoepithelial cells proliferate mitotically during regeneration of rat submandibular glands after atrophy.. Methods: The excretory duct of the right submandibular gland of rats was doubly ligated near the hilum with metal clips, which were removed after 7 days of ligation (day 0). The regenerating right submandibular glands were removed from 0 to 14 days after removal of the clips. The removed tissue was examined with immunohistochemical double staining for proliferating cell nuclear antigen (PCNA) as a marker of proliferating cells and actin as a marker of myoepithelial cells, as well as with transmission electron microscopy (TEM).. Results: The PCNA-positive myoepithelial cells were observed at the periphery of transitional duct-acinar structures, ducts and acini in the regenerating glands at every time-point, and the PCNA-labeling index of myoepithelial cells increased greatly especially between day 2 and 4. The mitosis of ...
TY - JOUR. T1 - Calcium‐dependent enhancement by carbachol of the VIP‐induced cyclic AMP accumulation in cat submandibular gland. AU - ENYEDI, PETER. AU - FREDHOLM, BERTIL B.. PY - 1984/4. Y1 - 1984/4. N2 - The interaction of two coexisting transmitters in the cat submandibular gland has been elucidated by studying effects of VIP and carbachol on cyclic AMP accumulation in isolated acini from the gland. Carbachol was found to potentiate the cyclic AMP increase induced by VIP by an atropine sensitive mechanism. The effect of carbachol on cyclic AMP accumulation was abolished by including EGTA in the incubation medium as was the carbachol mediated potentiation of VIP responses. The calmodulin inhibitor trifluoperazine had a similar, but less marked effect. The effect of carbachol was mimicked by phenylephrine (30 μM) and by the calcium inophore A 23187 (3 μM), and also by ethanol in a concentration reported to enhance membrane fluidity. The phospholipase A2 inhibitor, mepacrine, tended to ...
The aim of this research I to determine, at the ultrastructural level, the localization and distribution of individual sugar sugar moieties and terminal sialylated sequences of the secretory products of the mouse SMG of both sexes. We therefore investigated the lectin receptors of control and sialidase-treated sections by a post-embedding approach using horse radish peroxidase (HRP)-conjugates (PNA, DBA, LTA, WGA, Con A), anti-HRP antibody and protein A-gold. Qualitative and quantitative differences occurred in the acinar products of males and females. Sialidase digestion revealed that the acceptor sugar for terminal sialic acids exhibit a different expression in the electron-lucent granules of acinar cells. The occurrence of terminal sialic acid-alpha-N-acetylgalactosamine disaccharide was prominent in male acinar cells in contrast to a modest presence in female. In addition, the terminal sequence sialic acid beta-galactose showed a homogeneous location in the male secretory products and a ...
Mouse submandibular gland (SMG) begins its development at embryonic day 11 when oral epithelium grows into the underlying mesenchyme. The metabolic pathway of protein N-glycosylation is critical for SMG development and partial inhibition of DPAGT1, the gene that initiates N-glycosylation, drives cytodifferentiation of ductal structures. We have shown that DPAGT1 is a target of the canonical Wnt signaling pathway and that DPAGT1 regulates E-cadherin-mediated cell-cell adhesion, required for the survival of differentiating duct cells. Recently, the Hippo pathway has been shown to be critical for tissue development by regulating Wnt signaling and establishing apical-basal polarity. TAZ is a Hippo pathway transcription factor and polarity enhancer that also serves as a mechanosensor of the extracellular matrix. Objective: We investigated whether the Hippo pathway participated in SMG development and if it interacted with the metabolic pathway of N-glycosylation. Method: SMGs were dissected from mice ...
OBJECTIVE: To investigate protein content of saliva produced in vitro by samples of human submandibular gland following stimulation with the muscarinic agent carbachol. DESIGN: Tissue samples, obtained at surgery from seven patients and showing normal morphological appearance, were tested for 30 min: in absence of carbachol and atropine; in presence of carbachol (10 microM); in presence of carbachol (10 microM) and atropine (20 microM); or in presence of just atropine (20 microM). Medium was analysed by high-performance liquid chromatography-mass-spectrometry. Neither before nor during surgery were the patients exposed to drug treatments that were likely to influence the in vitro secretion. RESULTS: Proline-rich proteins (PRP)-1 and -3, peptide PC and PB, statherin, cystatins SN, S1 and S2 were invariably found in control gland tissue medium. Mean concentrations of these proteins/peptides in the medium were non-proportionally elevated following carbachol exposure to the gland tissues. Difference ...
Hayashi and collaborators profiled the EGF dependent microRNAs of the fetal mouse submandibular gland at embryonic day 13. Avraham and colleagues, described a
J:79832 Hoffman MP, Kidder BL, Steinberg ZL, Lakhani S, Ho S, Kleinman HK, Larsen M, Gene expression profiles of mouse submandibular gland development: FGFR1 regulates branching morphogenesis in vitro through BMP- and FGF-dependent mechanisms. Development. 2002 Dec;129(24):5767-78 ...
Runx1 mediates the development of the granular convoluted tubules in the submandibular glands[1] "The mouse granular convoluted tubules (GCTs), which are only located in the submandibular gland (SMG) are known to develop and maintain their structure in an androgen-dependent manner. We previously demonstrated that the GCTs are involuted by the epithelial deletion of core binding factor β (CBFβ), a transcription factor that physically interacts with any of the Runt-related transcription factor (RUNX) proteins (RUNX1, 2 and 3). This result clearly demonstrates that the Runx /Cbfb signaling pathway is indispensable in the development of the GCTs. However, it is not clear which of the RUNX proteins plays useful role in the development of the GCTs by activating the Runx /Cbfb signaling pathway. Past studies have revealed that the Runx /Cbfb signaling pathway plays important roles in various aspects of development and homeostatic events. Moreover, the Runx genes have different temporospatial ...
Question - Swelling of submandibular salivary glands. Feel bumps on gland. Reason for swelling?. Ask a Doctor about diagnosis, treatment and medication for Salivary gland cancer, Ask an ENT Specialist
J:91711 Miyazaki Y, Nakanishi Y, Hieda Y, Tissue interaction mediated by neuregulin-1 and ErbB receptors regulates epithelial morphogenesis of mouse embryonic submandibular gland. Dev Dyn. 2004 Aug;230(4):591-6 ...
Objective To study the role of plus sijunzi decoction in the healing of experimental Pi-Xu syndrome combined with gastric ulcer. Methods Using hematoxylin-eosin staining, immunohistochemical technique, and Western-blotting to show the gastric mucosa structure, the phosphorylation level of ERK2 in the gastric mucosa, and the contents of EGF in the granular convoluted tubule (GCT) cells of the submandibular glands respectively. Results After treatment with plus sijunzi decoction, the phosphorylation level of ERK2 in the gastric mucosa decreased, and the contents of EGF in the GCT cells of the submandibular glands increased in contrast to the control group respectively. The recovery of gastric ulcer was accelerated. However,little change was found in spontaneously recovering group. Conclusion Plus sijunzi decoction accelerates the healing of pi-xu syntrom combined with gastric ulcer by mechanisms involving the increased release of EGF from the submandibular glands and the consequent up-regulation of
Aluminum absorption leads to deposits in several tissues. In this study, we have investigated, to our knowledge for the first time, aluminum deposition in the salivary glands in addition to the resultant cellular changes in the parotid and submandibular salivary glands in a model of chronic intoxication with aluminum citrate in rats. Aluminum deposits were observed in the parotid and submandibular glands. Immunohistochemical evaluation of cytokeratin-18 revealed a decreased expression in the parotid gland with no changes in the submandibular gland. A decreased expression of α-smooth muscle actin was observed in the myoepithelial cells of both glands. The expression of metallothionein I and II (MT-I/II), a group of metal-binding proteins, which are useful indicators for detecting physiological responses to metal exposure, was higher in both glands. In conclusion, we have shown that at a certain time and quantity of dosage, aluminum citrate promotes aluminum deposition in the parotid and submandibular
Submandibular gland removal is surgery to take out a saliva gland below the lower jaw. The gland may have been removed because of infection, a tumour, or a blocked saliva duct. A saliva duct is a tube that carries saliva from the gland into the mouth.. The area below your jaw may be sore for several days after your surgery. The area also may be slightly swollen or bruised. It will probably take 1 to 2 weeks for the cut (incision) the doctor made to heal.. If you have stitches in your incision, your doctor may need to remove them, or they may dissolve on their own. Ask your doctor about this. If your incision was closed with glue, the glue will peel off on its own in the weeks after your surgery.. ...
PLoS Comput Biol. 2013;9(11):e1003319. doi: 10.1371/journal.pcbi.1003319. Epub 2013 Nov 21. Research Support, N.I.H., Extramural
The majority of glands in the face are related to salivation and mastication, the exception being the lacrimal glands that lie in the superior and lateral aspect of the orbits. These produce tears than flow medially and drain through the puncta into the lacrimal sac and into the nasopharynx.. The salivary glands are the parotid, sublingual, and submandibular. The parotid gland lies just anterior to the ear, while the sublingual gland is within the floor of the mouth, and the submandibular glands live under the mandible. The parotid gland drains through Stensens duct which runs from the gland along the edge of the masseter and empties into the mount opposite by the second upper molar - this duct can often be disrupted by trauma. The submandibular glands are emptied by Whartons duct, exiting by the frenulum of the tongue.. ...
The ability of foetal rat salivary epithelium, particularly from the parotid gland, to develop morphogenetically and functionally (amylase activity) in various mesenchymes, and the quantitative effects of altering mesenchymal mass on the development of the parotid epithelium, have been studied in vitro.. Both parotid and submandibular epithelial rudiments were able to undergo morphogenesis and subsequent cytodifferentiation in their own and in the reciprocal mesenchyme. The growth of the explant and the arrangement of the acini were governed by the mesenchyme, submandibular mesenchyme supporting the development of more acini, which were more closely packed, than parotid mesenchyme. The functional product of the epithelium was not qualitatively affected, amylase activity being developed only by parotid epithelium, whether in its own or in submandibular mesenchyme.. Amylase activity was greater when the epithelium from a single parotid rudiment was recombined with submandibular mesenchyme than ...
A membrane protease possessing thrombin-like activity was purified to homogeneity from mitochondria of rat submaxillary gland. The molecular mass of the enzyme was determined to be 45 kDa by SDS/PAGE under reducing conditions and by gel filtration on a Sephadex G-100 column. The enzyme is a glycoprotein and has an isoelectric point of 3.25. Maximum activity was observed at pH 10.5. Inhibition by di-isopropyl fluorophosphate, benzamidine, aprotinin and antipain suggested the enzyme to be a serine protease. Other inhibitors such as EDTA, soya-bean trypsin inhibitor, lima-bean trypsin inhibitor, TosLysCH2Cl and chymostatin did not alter the activity. The enzyme showed affinity towards different synthetic substrates (p-nitroanilide derivatives) containing arginine at the P1 position. Kinetic studies revealed that kcat./Km was highest with the substrate N-Bz-Phe-Val-Arg-p-nitroanilide. The enzyme exhibits significant plasma-coagulating activity. The coagulation initiated by the enzyme was not altered ...
Immediately anteromedial to the right submandibular gland is a circumscribed rounded 6 mm calcification compatible with a sialolith. The proximal most aspect of
Michael, did those research studies mention anything on time frames...?. While my time on here, not every one lost their saliva, so I guess dry mouth is a matter of interpretation. Definitely not 100% completely lost salivary function permanantly, or even temporary for that matter.. Most have and did lose some if not all salivary function as a result of rads. But many of those like myself, and several others have gotten nearly all (if not all) of their saliva back. A few didnt lose it, and at least one I think said it increased.... For me I have around 90 - 95% back I would say... I only get dry during sleep at night. The rest of the time I think Im probably 100% or close enough I cant tell.. Studies are usually geared with an objective and agenda (from what I have found)... And one thing youll hear here often, we are all different... Personally from my four plus years on here, the odds of losing your saliva long term enough to be significant is very low.. Are you or most going to lose ...
It is supplied by branches from the sumandibular ganglion. These branches convey a) secretomotor fibres; (b)sensory fibres from the lingual nerve; and (c) vasomotor sympathetic fibres from the plexus on the facial artery ...
ImSAIDs are a class of peptides being developed by IMULAN BioTherapeutics, LLC, which were discovered to have diverse biological properties, including anti-inflammatory properties. ImSAIDs work by altering the activation and migration of inflammatory cells, which are immune cells responsible for amplifying the inflammatory response.[10][11] The ImSAIDs represent a new category of anti-inflammatory and are unrelated to steroid hormones or nonsteroidal anti-inflammatories. The ImSAIDs were discovered by scientists evaluating biological properties of the submandibular gland and saliva. Early work in this area demonstrated that the submandibular gland released a host of factors that regulate systemic inflammatory responses and modulate systemic immune and inflammatory reactions. It is now well accepted that the immune, nervous, and endocrine systems communicate and interact to control and modulate inflammation and tissue repair. One of the neuroendocrine pathways, when activated, results in the ...
The kinetics of K+ release from an in vitro system of rat submaxillary gland slices were studied after stimulation with parasympathomimetic secretagogues. The slices were incubated at 37degreesC in an oxygenated, enriched Krebs-Ringer bicarbonate medium in the presence and in the absence of Ca++ and of ouabain and, in some experiments, in the presence of the specific antagonists atropine (5 x 10(-6) and 2 x 10(-5) M), phentolamine (2 x 10(-5) M) or propranolol (2 x 10(-5) M. K+ release was elicited by the addition of acetylcholine (2 x 10(-5) M), pilocarpine (2 x 10(-5) M) and carbamylcholine (10(-9) to 2 x 10(-5) M). The results demonstrate that: 1) The selective stimulation of cholinergic receptors induces a rapid net release of K+ from the slices. After 10 minutes of incubation, the percent K+ released after a 2 x 10(-5) M dose of each of the three secretagogues was, respectively, 20.8%, 15.5%, and 19%. 2) The response to carbamylcholine does not occur when Ca++ is absent from the medium and ...
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Journal of Clinical and Diagnostic Research aims to publish findings of doctors at grass root level and post graduate students, so that all unique medical experiences are recorded in literature.
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Differentiation of salisphere into acinar cells. (A) Amylase expressing cells (AC) in submandibular gland tissue (Tissue) were also present at the onset of cult
Aside from the blenderized diet, he overall is doing well except for some severe choking. Dear Sons throat muscles have deteriorated with is progressive disease and he is unable to process his own secretions. Normal people swallow their own saliva throughout the day but he cant swallow (which is why he uses a feeding tube) so he is choking on his own saliva. We have tried the normal treatments of Robinul and even botox injections in 2008 for him but they both failed. We had been using a Scopolamine patch for many years up until the manufacturing shortage. Once we stopped the patch, we learned that it was actually causing seizures for Dear Son. When we tried the patch after the manufacturing issue was resolved, we could tell that it was causing seizures for him so now we cant use that. We met with an ENT to discuss the possibility of the removal of his submandibular glands and one of his parotid glands. I thought perhaps since Dear Son was healthy, this might be a good time to consider this ...
Mutants of the second type lacked flagella and were not able to colonize the hyphae or inhibit fungal growth. The paired sample (study and comparison groups) was noted to be similar to TSCS elderly profiles for these subscales. On the other purchasing cialis on the internet hand, the ocular findings corroborate the previous researches. Effects of reserpine and isoproterenol on elemental distribution in submandibular gland of is there a generic cialis rat. Five Cases of Infection of the Urinary Tract Due to a Member of the Group of Bacilli Named after Morgan. Such plasticity and its kinetics can have a crucial functional role, and their characterization has taken center stage in protein research.. A single amino acid is critical for the expression of B-cell epitopes on the helicase domain of the otc cialis pestivirus NS3 protein. Secondary involvement of the thyroid gland by malignant metastases is uncommon.. Higher volume injections resulted in excessive periprostatic extravasation, whereas ...
Study to evaluate the toxic response of associated with infusion of the submandibular gland with an adenoviral factor encoding the gene for human growth hormone (HGH).. ...
Proximal femoral focal deficiency, PFFD, is a congenital anomaly of the pelvis and proximal femur which causes hip deformity and shortening and altered function of the involved lower extremity. The condition may be unilateral or bilateral and is often associated with other congenital anomalies. Embryology: The developing human embryo first shows evidence of limb buds at the 5 millimeter crown-rump stage.As the apical mesoderm proliferates the limb is laid down in a proximo-distal fashion to be complete at the 12 millimeter stage. Elements of the ileum and proximal femur develop from a common cartilaginous anlage, with subsequent cleft formation to create a joint cavity. This means that if an acetabulum is seen in radiograph at any time in the first year of life a femoral head and neck will be present also, even if not evident in the radiograph. Etiology: Numerous agents including irradiation, anoxia, ischemia, mechanical or thermal injury, bacterial toxins, viral infection, chemicals and ...
Patient Presentation A 25-day-old female was transferred to a regional childrens hospital after a 3 day history of right submandibular swelling. Originally she was seen again by her local pediatrician who gave her 1 dose of ceftriaxone and then began Amoxicillin-Clavulanic acid twice a day. She was re-seen the following day without any change in…
TY - JOUR. T1 - Oncocytic variant of mucoepidermoid carcinoma of submandibular gland. T2 - An unusual clinical and morphological entity. AU - Krishnanand, Geetha. AU - Kaur, Manpreet. AU - Rao, Ravikala V.. AU - Monappa, Vidya. PY - 2007/7/1. Y1 - 2007/7/1. N2 - In this case report we describe a rare tumor - Oncocytic variant of Mucoepidermoid carcinoma of the submandibular salivary gland with a review of the literature. Oncocytic metaplasia in salivary glands is a benign change that is associated with increasing age and also seen in a few salivary gland neoplasms, which include oncocytoma, Warthins tumor, and the rare, oncocytic carcinoma. Oncocytic differentiation in mucoepidermoid carcinoma (MEC) is uncommon. Only twelve well-documented cases of oncocytic MEC have been reported previously all of which occurred in the parotid gland. To the best of our knowledge this is the first case of oncocytic mucoepidermoid carcinoma involving the submandibular salivary gland. The recognition of this ...
mouse epidermal growth factor binding protein: four different mouse proteins have been identified as EGF-BP; each is a kallikrein that associates with EGF to form a high molecular weight complex in male mouse submandibular glands (separate SCRs for each of four proteins exist)
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The Ren-1 locus of mice encodes the protease renin, which with converting enzyme processes angiotensinogen to the potent vasopressor angiotensin II. Some strains of mice appear to carry a duplication of the renin structural gene (Ren-2) near the Ren-1 locus. Strains with the gene duplication can exhibit as much as 100-fold higher levels of submaxillary gland renin compared to strains with a single gene copy. In contrast, kidney renin levels appear to be unaffected by the gene duplication. Sequence analysis of a 319 bp renin cDNA recombinant isolated from a kidney library from the two-gene strain DBA/2Ha corresponds to a transcript of the Ren-1 gene. Moreover, a single base substitution of A for G at residue #996 in the kidney renin mRNA creates a potential glycosylation recognition site that may, in part, account for the differential glycosylation of kidney and submaxillary gland renins. In addition, our tissue surveys indicate that mature mRNAs from the Ren loci are detectable in adrenal gland ...
The submandibular ganglion is one of four parasympathetic ganglia of the head and neck. It receives parasympathetic fibres from the facial nerve. Gross anatomy small ganglion suspended from the undersurface of the lingual nerve inferior to sub...
It has been reported that immunization against tonin normalizes blood pressure, and that sialoadenectomy, during which the tonin-rich salivary glands are removed, decreases blood pressure in one-kidney, one-clip hypertension. To investigate the role of tonin on this form of hypertension further, we actively immunized one-kidney, one-clip hypertensive rabbits with tonin and measured both the blood pressure response and the titer of antibodies raised against tonin. In addition, because sialoadenectomy may alter food intake, we assessed the effect of sialoadenectomy on the blood pressure of one-kidney, one-clip hypertensive rats fed a liquid diet to facilitate eating. After immunization, all rabbits developed antitonin-antibody titers ranging from 1:300 to 1:56,000. However, in none of the rabbits did the blood pressure decrease significantly (114 +/- 3 mm Hg before immunization; 129 +/- 6 mm Hg at 16 weeks after immunization). In one-kidney, one-clip hypertensive rats, sialoadenectomy did not ...
To elucidate the role of extracellular matrix and basement membrane associated proteins at the ectodermal-ectomesenchymal interface of developing salivary glands, human fetal submandibular glands (n=100), gestational age varying from 10 to 40 weeks, were evaluated for expression of tenascin, type IV collagen and laminin by using immunohistochemical methods and the results were compared with their expression in adult glands (n=20). Immunoreactive tenascin was present in the epithelial-mesenchymal interface of developing salivary ducts beginning from 10 weeks of gestational age during the early developing stages. In the late developmental stages, the intensity of immunoreaction was reduced around the proximal striated ducts and no immunoreaction was seen around the intercalated duct and acinar cells. Adult submandibular glands had immunoreactive tenascin confined to the periductal connective tissue of distal segment of striated and excretory ducts. Type IV collagen and laminin were observed in the ...
Exemplary methods for affecting conduction and/or operation of the AV node and/or AV bundle. Various exemplary methods include delivery of one or more stimulation pulses to affect conduction and/or operation of an AV node and/or AV bundle. Such delivery optionally stimulates nerves and/or tissue. Nerve stimulation optionally includes parasympathetic nerve stimulation to decrease conduction of the AV node and/or AV bundle. Tissue stimulation optionally causes tissue to enter a refractory period. Various exemplary methods include delivering one or more stimulation pulses during postinspiration. Other exemplary methods and/or devices are also disclosed.
A gland is an organ in an animals body that synthesizes a substance for release such as hormones, often into the bloodstream (endocrine gland) or into cavities inside the body or its outer surface (exocrine gland). Humans have a large variety of glands, from the pituitary gland in the brain, to sweat glands over the bodys skin that release perspiration to regulate the bodys temperature. Other well known glands include the adrenal glands, the prostate gland, the thyroid gland, the pineal gland, the thymus and mammary gland. Two other paired glands are the parotid glands and submandibular glands, both involved in saliva production. Template:Anatomy-stubde:Dr se es:Gl ndula eo:Glando fr:Glande ia:Glandula he:בלוטה lt:Liauka pl:Gruczoł ...
MCMV has broad tissue tropism, and can infect the hosts epithelial cells such as salivary gland tissue, as well as macrophages and lymphoid cells. In natural infections, no clinical symptoms may be detected. The submandibular salivary gland is the primary site of lesions; other salivary glands are rarely involved. A substantial percentage of infected mice may eventually develop latent infections, and the virus may be transmitted to other mice through biting ...
The basement membrane separates the epithelium from the surrounding mesenchyme and plays an essential role in the development of various epithelial-mesenchymal organs. Among these, the submandibular salivary gland (SMG) has been chosen to review the expression patterns and roles of the epithelial...
Epithelia from lung rudiments in which secondary bronchial buds are already established (14th and 13th gestational day for rat and mouse respectively) are able to undergo branching morphogenesis and cytodifferentiation in submandibular mesenchyme in vitro, whereas lung epithelium from one day younger foetuses rarely gives a morphogenetic response to submandibular mesenchyme and usually differentiates into primary (non-budding) bronchial epithelium.. The failure of 13-day rat lung epithelium to respond to submandibular mesenchyme can be prevented by peeling off the submandibular mesenchyme from the lung epithelium after 2½ days culture and replacing the same mesenchyme, or renewing it with fresh salivary mesenchyme ex vivo. Changes in the epithelial contour are visible by 10 h and buds form within 24 h; this is followed by branching morphogenesis in more than 66% of the samples.. The number of cells in S-phase in the epithelium is doubled within 3 to 5 h after the operation and the number of ...
Saliva is produced primarily by the parotid, sublingual and submandibular glands. Salivary fluid may contain various amounts of hormones, depending on the ability of these hormones to leave the blood, penetrate through or around the salivary cell membranes, and diffuse into the saliva. Lipid-soluble hormones can pass directly through cell membranes via passive diffusion and are commonly found in the saliva. Small, water-soluble hormones may be able to enter the saliva through the tight junctions between salivary cells in a process called ultrafiltration. Salivary cells do not appear to use active transport to place hormones into the saliva.. Salivary tests may help to identify hormonal imbalances in either females or males which can occur at any age and effect how a person feels on a daily basis. The slightest dysfunction of an individuals hormones can result in weight gain, mood changes, low libido, poor memory or concentration, depression, bone loss, migraines or headaches and sleep ...
Purified epidermal growth factor (EGF), isolated from mice submaxillary glands, was used to study regeneration of rabbit corneal epithelium. The progressive decrease in area of standardized 7 mm. central comeal epithelial wounds was determined by serial standardized photography. The projected fluorescein-stained area was measured by planimetry. It has been found that EGF in the concentrations studied (0.05 mg. per milliliter; 0.5 mg. per milliliter; and 2.0 mg. per milliliter) when given topically four times daily, increased the corneal epithelial healing rate compared to saline controls. A 40-fold variation of EGF concentrations failed to effect a statistically significant change in corneal epithelial healing rate. No sign of toxicity was detected clinically and histologically with topical application of EGF on rabbit corneas with intact epithelium and on corneas denuded of epithelium ...
Title: Endothelial cell signaling in submandibular salivary gland regeneration (A. Altrieth), Regulation of a ncRNA involved in dosage compensation (S. Breznak), Alternative ribosomes in Mycobacterial species (J. Corro), Using single-molecule experiments to calibrate nucleic acid base-stacking in molecular simulations (K. Dill) and HNA- A new oligonucleotide (A. Hoy). Date: October 3, ...
Intranasal Administration of Mesenchymoangioblast-Derived Mesenchymal Stem Cells Abrogates Airway Fibrosis and Airway Hyperresponsiveness Associated with Chronic Allergic Airways Disease Researchers assessed the in vivo efficacy of induced pluripotent stem cell and mesenchymoangioblast-derived mesenchymal stem cells (MCA-MSCs) on airway remodeling in a murine model of chronic allergic airways disease/asthma. Apart from epithelial thickness, all other parameters measured were significantly, although not totally, decreased by intravenous delivery of MCA-MSCs to ovalbumin-injured mice. [FASEB J] Abstract Identification and Characterization of a Rich Population of CD34+ Mesenchymal Stem/Stromal Cells in Human Parotid, Sublingual and Submandibular Glands Very little is known about the phenotype, distribution and molecular nature of mesenchymal stem/stromal cells (MSCs) in freshly isolated human salivary glands (SGs) as most reports have focused on the analysis of cultured MSCs. The authors results ...
Lethality: Ras signaling promotes the excretory duct cell fate, and mosaic analysis suggested that loss of this one specific cell can account for the zygotic lethality of let-60 null mutants (Yochem et al., 1997). The excretory duct cell is required for osmoregulation (Nelson and Riddle, 1984). let-60 loss-of-function mutants, which lack the excretory duct cell, die as "rod-like" larvae with a fluid-filled appearance (Figure 2). let-60 gain-of-function mutants sometimes have two excretory duct cells (Yochem et al., 1997). Ras signaling may have other (maternally-rescued) essential roles in addition to excretory duct formation, since mutations in let-23/EGFR, egl-15/FGFR and ptp-2/SHP-2 cause distinct "scrawny" and lethal defects that are rescued by constitutive forms of LET-60 Ras (DeVore et al., 1995; Gutch et al., 1998; Koga and Ohshima, 1995). Hyperactivation of egl-15/FGFR or let-60/Ras can also lead to lethality with a "Clear", fluid-filled appearance (Kokel et al., 1998; Schutzman et al., ...
The rat kallikrein family consists of multiple closely related proteins. A method for demonstration and identification of kallikrein-like proteins has been developed based on their differences in isoelectric point and their immunological similarity. The method, which involved separation in flat-bed isoelectro-focusing gels (pH range 3-9) and detection by immunoblotting using polyclonal antiserum against one of the family members, has been used in the present study to detect kallikrein-like proteins in the rat prostate. Nine immunoreactive kallikrein-like protein bands were detected with pI ranging from 5.30 to 8.35. Of these, six were completely purified and three were partially purified. Two proteins (pI 5.30 and 6.75-6.90) corresponded to protein bands in gels of rat submandibular-gland extracts, and were identified by partial amino acid sequence analysis as rK8 and rK9 respectively. In addition, sequence analysis revealed complete sequence similarity between rK9 and the immunoreactive ...