During incubation of antipyrine, but not amidopyrine, 4-aminoantipyrine and 4-leucylaminoantipyrine, with rat liver microsomaland cytosol fractions in the presence of NADPH-generating system a reactive metabolite, which binds with glutathione is form
In order to benefit maximally from large scale molecular biology data generated by recent developments, it is important to proceed in an organized manner by developing databases, interfaces, data visualization and data interpretation tools. Protein subcellular localization and microarray gene expression are two of such fields that require immense computational effort before being used as a roadmap for the experimental biologist. Protein subcellular localization is important for elucidating protein function. We developed an automatically updated searchable and downloadable system called model organisms proteome subcellular localization database (MEP2SL) that hosts predicted localizations and known experimental localizations for nine eukaryotes. MEP2SL localizations highly correlated with high throughput localization experiments in yeast and were shown to have superior accuracies when compared with four other localization prediction tools based on two different datasets. Hence, MEP2SL system may ...
TRIM22 alters the sub-cellular localization of Gag protein.A) Analysis of Gag localization by fluorescence microscopy. HOS-CD4/CXCR4 cells were co-transfected w
Cells of virtually all organs and tissues are known to release large numbers of subcellular particles called extracellular vesicles (EVs) into bodily fluids such as blood and urine. There has been a growing interest in ...
Cheng, X., Xiao, X. and Chou, K.C. (2018) pLoc-mGneg Predict Subcellular Localization of Gram-Negative Bacterial Proteins by Deep Gene Ontology Learning via General PseAAC. Genomics, 110, 231-239.
Subcellular localization of the Nramp1 protein in macrophages. Peritoneal macrophages from normal 129/sv mice (A) and from 129/sv Nramp1−/− mutants (B) w
Defining the subcellular localisation of the proteome for an organism of interest is a critical next step following genome sequencing
The cell array-based protein expression and detection approach is a cost-effective platform for large-scale functional analyses, including protein subcellular localization and cell phenotype screening. The results from this study reveal novel functional features of human Chr21 proteins, which should …
В месте субклеточных фракционирования клеток млекопитающих на микроскопе покровные позволяет визуализировать белки...
Used with organs, cells and subcellular fractions, organisms, and diseases for biochemical changes and metabolism. It is used also with drugs and chemicals for catabolic changes (breakdown of complex molecules into simpler ones). For anabolic processes (conversion of small molecules into large), BIOSYNTHESIS is used. For enzymology, pharmacokinetics, and secretion use the specific subheadings. . ...
BioreclamationIVT is the complete resource for all biologicals including human clinical samples, hepatocytes, subcellular fractions and biological matrices.
BioreclamationIVT is the complete resource for all biologicals including human clinical samples, hepatocytes, subcellular fractions and biological matrices.
Part of a whole; part of a set; equivalent fractions; comparing; ordering; lowest terms; renaming improper fractions and mixed numbers; Adding and subtracting like and unlike fractions; adding and subtracting mixed numbers with like and unlike fractions; Multiplying a whole number times a fraction and a fraction times a whole number; multiplying a fraction times a fraction; multiplying a whole number times a mixed number; multiplying a mixed number times a mixed number; Dividing a whole number by a fraction; dividing a fraction by a fraction; reciprocals. ...
Caltag Medsystems supply whole cell extraction, nuclear extract, cell fractions and lysates from multiple species including human, mouse, rat, monkey, dog, whole cell extraction, nuclear extract.
Get your kid in on the fraction action! Shell get great fraction practice with these worksheets on dividing, multiplying, adding and subtracting fractions.
Title: A Research on Bioinformatics Prediction of Protein Subcellular Localization. VOLUME: 4 ISSUE: 3. Author(s):Gang Fang, Guirong Tao and Shemin Zhang. Affiliation:Department of Life Science, Xian University of Arts and Science, Xian 710065, China.. Keywords:Bioinformatics, prediction, protein subcellular localization, localizome, proteomics, database. Abstract: Protein subcellular localization is one of the key characteristic to understand its biological function. Proteins are transported to specific organelles and suborganelles after they are synthesized. They take part in cell activity and function efficiently when correctly localized. Inaccurate subcellular localization will have great impact on cellular function. Prediction of protein subcellular localization is one of the important areas in protein function research. Now it becomes the hot issue in bioinformatics. In this review paper, the recent progress on bioinformatics research of protein subcellular localization and its prospect ...
Experimentally determining the subcellular localization of a protein can be a laborious and time consuming task. Immunolabeling or tagging (such as with a green fluorescent protein) to view localization using fluorescence microscope are often used. A high throughput alternative is to use prediction. Through the development of new approaches in computer science, coupled with an increased dataset of proteins of known localization, computational tools can now provide fast and accurate localization predictions for many organisms. This has resulted in subcellular localization prediction becoming one of the challenges being successfully aided by bioinformatics, and machine learning. Many prediction methods now exceed the accuracy of some high-throughput laboratory methods for the identification of protein subcellular localization.[1] Particularly, some predictors have been developed[2] that can be used to deal with proteins that may simultaneously exist, or move between, two or more different ...
Adjustments in protein subcellular localization and large quantity are central to biological regulation in eukaryotic cells. open-reading frame (ORF) is usually individually tagged, generating a full-length protein with a COOH-terminus GFP fusion, whose expression is usually driven by the endogenous ORF promoter (Huh 2003). We worked with the set of 4144 strains from the original collection previously annotated as having a visible GFP signal and representing 71% of the yeast proteome. We used this collection to measure the subcellular localization and large quantity of yeast proteins at the single-cell level in several conditions in period classes of up to 11 human resources (Chong 2015). A true number of existing sources present images of yeast cells from large-scale research. Some of these research assess phenotypes linked with evaluation of a little amount of morphologic features or indicators in a collection of mutants. Sources that home this type of data consist of SCMD (Saito 2004) and ...
The functional genomics approach requires systematic analysis of protein subcellular distribution and interaction networks, preferably by optimizing experimental simplicity and physiological significance. Here, we present an efficient in planta transient transformation system that allows single or m …
1. An attempt was made to study the rate of synthesis as well as the distribution of RNA in the various cellular fractions in the livers and kidneys of normal and castrated mice. 2. The tissue was fractionated by the procedure of Blobel & Potter (1967). By using this method it was not possible to find any pronounced difference in the relative proportions of RNA in isolated subcellular fractions when kidneys from normal and castrated mice were compared. On the other hand there was an indication of a shift toward the bound ribosomes in livers from normal mice in comparison with livers from castrated mice. 3. Disappearance of the radioactivity followed the pattern of the first-order reaction. Comparing the half-lives of RNA in liver and kidneys it was found that in the latter in both groups of animals half-lives were shorter no matter which cellular fraction was studied. 4. The half-lives for total homogenate RNA, total ribosomal RNA and low-molecular-weight RNA from kidneys of castrated mice were ...
Histochemical studies and electron microscopy of Bacillus subtilis revealed the presence of ATPase in various subcellular fractions. The enzyme was preferentially localized in mesosomes, cytoplasmic membrane, periplasmic space, and cell wall ...
Rodent skin (extrahepatic) subcellular fractions, including matching pooled IGS Sprague-Dawley rat microsomes and S9 from dermal tissue, characterized for in vitro ADME studies to test the biotransformation of transdermal xenobiotics.
Hello - Does anybody can help with working protocol about differential or gradient centrifugation for subcellular fractionation of insect cells (Sf9 cell line)? Also, any information about the densities or/and sedimentation coefficients of different types of INSECT cell organelles would be helpful. Unfortunately, I have no possibility for electron microscopy and no knowledge about marker enzymes for insect cell organelles to design a good experimental protocol for determining the insect cell subcellular fractions. Merike Meier, PhD student meerike at kbfi.ee ...
Sekisui XenoTech recently fulfilled its largest order of human liver subcellular fractions to date. A large pharmaceutical customer ordered over 30,000
1. Good laboratory practice, safety 2. Theoretical introduction to the laboratory tasks 3. Chromatography of biomacromolecules, evaluation of purification steps 4. Electrophoretic analysis of erythrocyte membrane proteins 5. Basic characterization of purified preparates of proteins 6. Enzyme kinetics (allosteric enzymes) 7. Modern methods of protein identification 8. Physical & chemical properties of proteins 9. Immunotechniques 10.Basic techniques of molecular biology 11.Isolation of subcellular particles 12.Study of basic metabolic events 13.Methods of determination of catalytical activity of enzymes 14.Evaluation, test and free discussion ...
This gene encodes a protein that plays an essential role in the production of iron-sulfur (Fe-S) clusters for the normal maturation of lipoate-containing 2-oxoacid dehydrogenases, and for the assembly of the mitochondrial respiratory chain complexes. Mutation in this gene has been associated with multiple mitochondrial dysfunctions syndrome-2. Two alternatively spliced transcript variants encoding different isoforms with distinct subcellular localization have been reported for this gene (PMID:21944046). [provided by RefSeq, Dec 2011 ...
We are studying the cellular expression of an enzyme which can exist both in the Golgi and at the level of the cell membrane. In vivo, subcellular fractionation is relatively straightforward using a differential gradient system. However, I have not come accross a parallel method for separating the plasma membranes and Golgi fractions from a cultured cell monolayer. This is a particular problem when the small amount of starting material is taken into consideration. Has anybody elso come accross this problem, or how it may be addressed? Kieran Breen Dept. of Pharmacology, University of Dundee, Ninewells Hospital & Medical School, Dundee DD1 9SY, Scotland, U.K. k.c.breen at dundee.ac.uk ...
Protein subcellular localization has been systematically characterized in budding candida using fluorescently tagged proteins. on automatically recognized cells and whose cell-stage dependency is definitely captured by a continuous model for cell growth. We show that it is possible to identify most previously recognized localization patterns inside a cluster analysis based on these features and that similarities between the inferred manifestation patterns contain more information about protein function than can be explained by a earlier manual categorization of subcellular localization. Furthermore the inferred cell-stage connected to each fluorescence measurement allows us to visualize large groups of 2C-C HCl proteins entering the bud at specific phases of bud growth. These correspond to proteins localized to organelles exposing the organelles must be entering the bud inside a stereotypical order. We also determine and organize a smaller group of proteins that show delicate differences in the ...
Membrane vesicle trafficking is the transport of cell products between subcellular components like the Endoplasmic Reticulum (ER) and Golgi apparatus.
Subcellular location of PGRMC2. Localized to the nuclear membrane, plasma membrane & cytoplasm. Analysis based on one antibody, HPA041172, using immunofluorescence in human cells
Subcellular location of IRS2. Mainly localized to the cytoplasm in human and mouse cells. Analysis based on one antibody, HPA054664, using immunofluorescence in human and mouse cells
Aim: To study the expression profile of the NaPi2b protein and its localization in breast, ovarian and lung cancer cells in relation to normal tissues adjacent
Due to the limited knowledge of the enzymes involved in the formation of LY404039, tissue homogenates were used in these studies instead of subcellular fractions to increase the possibility the enzymes involved would be represented. However, because these were human tissue preparations, the quality and treatment of the tissue, including the time interval between receipt and processing of the tissue, may be critical to the activity of these enzymes and may explain the observed large differences in Vmax values between donors (Tables 2 and 3). In accordance with this concept, the human plasma was freshly prepared and the range in Vmax values was substantially reduced.. Prior to incubations to establish kinetic parameters for pomaglumetad methionil hydrolysis, linear rate conditions for LY404039 formation were determined for each of the matrices by incubating multiple concentrations of pomaglumetad methionil, with varying protein concentrations of each matrix for increasing periods of time. Results ...
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A fraction by its very definition is a piece of data used to represent a fact. Fractions can be likened to percentages, ratios and decimals in that they all present a numeric piece of information considered as it relates to a whole data set. In this article...
0113] Free Neu5Gc can be taken up by human epithelial cells from an exogenous source and incorporated into different subcellular fractions. Evidence was presented suggesting that the small amounts of Neu5Gc found in some human tissues originated from dietary sources and showed that human Caco-2 cells (human epithelial cells from a primary colon carcinoma) in culture could metabolically incorporate free Neu5Gc, as determined by a Western blot of a total homogenate, using and anti-Neu5Gc antibody. Increasing incorporation of Neu5Gc was found in the total homogenate fraction of the cells over time, with the highest level reached after incubation with 3 mM Neu5Gc for 3 days. Moreover, Western blotting with an anti Neu5Gc antibody demonstrated metabolic incorporation of Neu5Gc into glycoproteins of these cells. The partitioning of the exogenous Neu5Gc into different subcellular fractions of these cells has now been studied. Prior to feeding, Caco-2 cells were split and cultured in human serum instead ...
Kernel discriminant analysis (KDA) is a dimension reduction and classification algorithm based on nonlinear kernel trick, which can be novelly used to treat high-dimensional and complex biological data before undergoing classification processes such as protein subcellular localization. Kernel parameters make a great impact on the performance of the KDA model. Specifically, for KDA with the popular Gaussian kernel, to select the scale parameter is still a challenging problem. Thus, this paper introduces the KDA method and proposes a new method for Gaussian kernel parameter selection depending on the fact that the differences between reconstruction errors of edge normal samples and those of interior normal samples should be maximized for certain suitable kernel parameters. Experiments with various standard data sets of protein subcellular localization show that the overall accuracy of protein classification prediction with KDA is much higher than that without KDA. Meanwhile, the kernel parameter of KDA
Pattern recognition and classification of images are key challenges throughout the life sciences. We combined two approaches for large-scale classification of fluorescence microscopy images. First, using the publicly available data set from the Cell Atlas of the Human Protein Atlas (HPA), we integrated an image-classification task into a mainstream video game (EVE Online) as a mini-game, named Project Discovery. Participation by 322,006 gamers over 1 year provided nearly 33 million classifications of subcellular localization patterns, including patterns that were not previously annotated by the HPA. Second, we used deep learning to build an automated Localization Cellular Annotation Tool (Loc-CAT). This tool classifies proteins into 29 subcellular localization patterns and can deal efficiently with multi-localization proteins, performing robustly across different cell types. Combining the annotations of gamers and deep learning, we applied transfer learning to create a boosted learner that can ...
TY - JOUR. T1 - The subcellular compartmentalization of arginine metabolizing enzymes and their role in endothelial dysfunction. AU - Chen, Feng. AU - Lucas, Rudolf. AU - Fulton, David. N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.. PY - 2013. Y1 - 2013. N2 - The endothelial production of nitric oxide (NO) mediates endothelium-dependent vasorelaxation and restrains vascular inflammation, smooth muscle cell proliferation, and platelet aggregation. Impaired production of NO is a hallmark of endothelial dysfunction and promotes the development of cardiovascular disease. In endothelial cells, NO is generated by endothelial nitric oxide synthase (eNOS) through the conversion of its substrate, L-arginine to L-citrulline. Reduced access to L-arginine has been proposed as a major mechanism underlying reduced eNOS activity and NO production in cardiovascular disease. The arginases (Arg1 and Arg2) metabolize L-arginine to generate L-ornithine and urea and increased expression of ...
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Fresh anterior pituitary glands from beef and pig were separated by differential centrifugation into subcellular fractions. Nuclei and debris were obtained at 700 g for 15 minutes, secretory granules at 7000 g for 20 minutes, mitochondria at 34,000 g for 15 minutes, and microsomes at 78,000 g for 3 hours. Electron micrographs were taken of the individual fractions. Each fraction was analyzed for nitrogen, pentosenucleic acid (PNA), and phospholipide. Beef and pig anterior lobes were quite similar in their intracellular composition as seen in the subcellular fractions. Succinic dehydrogenase was localized in mitochondria, while alkaline phosphatase was concentrated in the microsomes. A proteinase with pH optimum at 8.2 was exclusively localized. in microsomal and supernatant fractions. Acid phosphatase, acid ribonuclease, and acid proteinase were distributed among the subcellular fractions in another pattern, indicating the presence of a particle type distinct from mitochondria and microsomes. ...
TY - JOUR. T1 - SecA2 is distinct from SecA in immunogenic specificity, subcellular distribution and requirement for membrane anchoring in Streptococcus parasanguis. AU - Chen, Qiang. AU - Wu, Hui. AU - Kumar, Reetu. AU - Peng, Zhixiang. AU - Fives-Taylor, Paula M.. PY - 2006/11. Y1 - 2006/11. N2 - A secA2 gene is present in the genomes of a wide variety of Gram-positive bacteria. In Streptococcus parasanguis, a primary colonizer of the tooth surface, secA2 is involved in the secretion of a small group of proteins including the fimbrial adhesin, Fap1. Although the substrate specificity is different, SecA2 is predicted to be similar to SecA in structure and function based on the homology between these two proteins. In this study, polyclonal antibodies against SecA2 and SecA did not cross-react with each other, indicating that these two proteins possessed distinct immunogenic epitopes. Fractionation analysis demonstrated that SecA2 was not evenly distributed between the cytoplasmic membrane and ...
Eukaryotic cells contain a huge variety of internally specialized subcellular compartments. Stoichiogenomics aims to reveal patterns of elements usage in biological macromolecules. However, the stoichiogenomic characteristics and how they adapt to various subcellular microenvironments are still unknown. Here we first updated the definition of stoichiogenomics. Then we applied it to subcellular research, and detected distinctive nitrogen content of nuclear and hydrogen, sulfur content of extracellular proteomes. Specially, we found that acidic amino acids (AAs) content of cytoskeletal proteins is the highest. The increased charged AAs are mainly caused by the eukaryotic originated cytoskeletal proteins. Functional subdivision of the cytoskeleton showed that activation, binding/association, and complexes are the three largest functional categories. Electrostatic interaction analysis showed an increased electrostatic interaction between both primary sequences and PPI interfaces of 3D structures, in the
These intriguing results compelled us to develop a wide range of analytical tools to better study the intricacies of cellular biosynthetic machinery. We have perfected non-aqueous subcellular fractionation techniques in order to separate chloroplasts and vacuoles from cytosol. We are operating a metabolite profiling system, using GC-MS, which allows us to distinguish among large numbers of metabolites within each of these samples (subcellular fractions or tissue samples). In excess of 300 compounds can be profiled in this way , 100 of these compounds having known chemical structures. A further experimental development that we are exploring is the use of chemically-inducible promoters to drive transgene expression in a controlled manner in order to study perturbations of metabolism on a temporal basis. In recent years we have additionally established an RT-PCR platform for tomato transcription factors and sensitive methods for following the metabolism of stable isotope labeled substrate and an ...
Cytokinin dehydrogenase (CKX; EC 1.5.99.12) degrades cytokinin hormones in plants. There are several differently targeted isoforms of CKX in plant cells. While most CKX enzymes appear to be localized in the apoplast or vacuoles, there is generally only one CKX per plant genome that lacks a translocation signal and presumably functions in the cytosol. The only extensively characterized maize CKX is the apoplastic ZmCKX1; a maize gene encoding a non-secreted CKX has not previously been cloned or characterized. Thus, the aim of this work was to characterize the maize non-secreted CKX gene (ZmCKX10), elucidate the subcellular localization of ZmCKX10, and compare its biochemical properties with those of ZmCKX1. Expression profiling of ZmCKX1 and ZmCKX10 was performed in maize tissues to determine their transcript abundance and organ-specific expression. For determination of the subcellular localization, the CKX genes were fused with green fluorescent protein (GFP) and overexpressed in tomato hairy ...
To elaborate on off-flavour development in dehydrated potato granules, lipids in subcellular particles and membrane systems of the tuber were investigated. Lipid acyl-hydrolase and lipoxygenase...
The distribution of survival times of rats subjected to acceleration stress of 20 Gz conforms to the Roginsky-Zeldovich or Elovich equation. This equation is derived from the hypothesis of electron or ion conduction across an activation energy barrier at the surface of a cell or subcellular particle, which suggests that tolerance to this acceleration stress is dependent upon such a biophysical process. Author
The more proteins diverged in sequence, the more difficult it becomes for bioinformatics to infer similarities of protein function and structure from sequence. The precise thresholds used in automated genome annotations depend on the particular aspect of protein function transferred by homology. Here, we presented the first large-scale analysis of the relation between sequence similarity and identity in subcellular localization. Three results stood out: (1) The subcellular compartment is generally more conserved than what might have been expected given that short sequence motifs like nuclear localization signals can alter the native compartment; (2) the sequence conservation of localization is similar between different compartments; and (3) it is similar to the conservation of structure and enzymatic activity. In particular, we found the transition between the regions of conserved and nonconserved localization to be very sharp, although the thresholds for conservation were less well defined than ...
Systems Biology requires comprehensive systematic data on all aspects and levels of biological organization and function. In addition to information on the sequence, structure, activities and binding interactions of all biological macromolecules, the creation of accurate predictive models of cell behaviour will require detailed information on the distribution of those molecules within cells and the ways in which those distributions change over the cell cycle and in response to mutations or external stimuli. Current information on subcellular location in protein databases is limited to unstructured text descriptions or sets of terms assigned by human curators. These entries do not permit basic operations that are common to other biological databases, such as measurement of the degree of similarity between the distributions of two proteins, and they are not able to fully capture the complexity of protein patterns that can be observed. The field of location proteomics seeks to provide automated, ...
Chlorotrifluoroethene is nephrotoxic in rats, and glutathione S-transferase-catalyzed S-(2-chloro-1,1,2-trifluorethyl)glutathione (CTFG) formation is the initial step in its bioactivation. CTFG biosynthesis and the activities of cytosolic and microsomal glutathione S-transferases were measured in rat and human hepatocytes and in human hepatoma-derived Hep G2 cells. Hepatocytes of , or = 88% viability were obtained from rat or human liver slices by collagenase or collagenase+dispase digestion, respectively. Hep G2 cells were grown in modified Earles medium supplemented with 10% (v/v) fetal calf serum. Cells and subcellular fractions were exposed to chlorotrifluoroethene, and CTFG formation was quantified by HPLC. Both human liver and Hep G2 cell subcellular fractions catalyzed CTFG formation, and human and rat microsomal fractions exhibited higher specific activities than cytosolic fractions with chlorotrifluoroethene as the substrate. Time-dependent formation of CTFG was observed in all cell ...
Here we present protocols for detergent-free homogenization of cultured mammalian cells based on nitrogen cavitation and subsequent...
Ive fractionated some brain tissue using a kit from invitrogen which involves using different buffers and centrifugation speeds on the brain homogenate to fractionate the samples into a nuclear, cytosolic, cytoskeletal and membrane fraction. I confirmed fractionation had worked by running the fractions on a gel and western blotting for cytochrome C (membrane) NeuN (nuclear marker specific for neurons)and neurofilaments (cytoskeletal fraction). These gave the expected results - but when I blotted for my protein of interest - which by microscopy is both nuclear and cytoplasmic - it appeared in every fraction except the nuclear fraction ...
TY - JOUR. T1 - Phospholipase A in rat and chicken liver. T2 - Subcellular distribution and effects of treatment with estradiol and progesterone. AU - Gabriel, L.. AU - Shakir, K. M.M.. AU - Maor, D.. AU - Margolis, S.. PY - 1979/1/1. Y1 - 1979/1/1. UR - http://www.scopus.com/inward/record.url?scp=0018390367&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0018390367&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0018390367. VL - 38. SP - No. 77. JO - Federation proceedings. JF - Federation proceedings. SN - 0014-9446. IS - 3 I. ER - ...
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NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state ...
Testing against regional requirements using fractionation techniques to separate contaminants by compound type, carbon range or specific compounds.
Wanstrup, J and Ranlov, P, Transfer amyloidosis. Ultrastrusture of the transferred subcellular fractions. (1968). Subject Strain Bibliography 1968. 1145 ...
Biology of Reproduction contains original scientific research on a broad range of topics in the field of reproductive biology, as well as minireviews.
Obtenez ceci dans une bibliothèque! New-Opathies : an Emerging Molecular Reclassification of Human Disease. [Errol C Friedberg; World Scientific (Firm);] -- This book presents new insights into the etiology and pathogenesis of systemic diseases recently discovered to be due to specific defects in molecular assemblies, organelles, or other subcellular ...
For fractionation we use a Qiagen kit, but somehow I have a feeling that it must be technical problem of correct separation of cellular fractions. We suspect also that the protein can be attached to nuclear envelope and nuclear location can be linked to the developmental stage of the cells ...
Hong-Bin Shen and Kuo-Chen Chou, Gpos-mPLoc: A Top-Down Approach to Improve the Quality of Predicting Subcellular Localization of Gram-Positive Bacterial Proteins, Protein and Peptide Letters, 2009, 16, 1478-1484 ...
Decimal to Fraction Number calculator - online basic math function tool to convert decimal point number 0.67 to fraction equivalent. Simplest fraction: 0.67 = 67/100 = 67/100; percentage: 0.67 = 67/100 or 67%
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