TY - JOUR. T1 - Combined introduction of Bmi-1 and hTERT immortalizes human adipose tissue-derived stromal cells with low risk of transformation. AU - Tátrai, Péter. AU - Szepesi, Áron. AU - Matula, Zsolt. AU - Szigeti, Anna. AU - Buchan, Gyöngyi. AU - Mádi, András. AU - Uher, Ferenc. AU - Német, Katalin. PY - 2012/5/25. Y1 - 2012/5/25. N2 - Adipose tissue-derived stromal cells (ASCs) are increasingly being studied for their usefulness in regenerative medicine. However, limited life span and donor-dependent variation of primary cells such as ASCs present major hurdles to controlled and reproducible experiments. We therefore aimed to establish immortalized ASC cell lines that provide steady supply of homogeneous cells for in vitro work while retain essential features of primary cells. To this end, combinations of human telomerase reverse transcriptase (hTERT), murine Bmi-1, and SV40 large T antigen (SV40T) were introduced by lentiviral transduction into ASCs. The resulting cell lines ASC ...
TY - JOUR. T1 - Adipose stromal cells differentiation toward smooth muscle cell phenotype diminishes their vasculogenic activity due to induction of activin A secretion. AU - Merfeld-Clauss, Stephanie. AU - Lease, Benjamin R.. AU - Lu, Hongyan. AU - March, Keith L.. AU - Traktuev, Dmitry O.. PY - 2017/11. Y1 - 2017/11. N2 - Adipose stromal cells (ASCs) support endothelial cell (EC) vasculogenesis through paracrine and cell-contact communications. In addition, ASCs differentiate towards the smooth muscle cell (SMC) phenotype under different stimuli, which prompted their use as a source of mural cells in fabricating small calibre vessels. How ASCs SMC-lineage commitment affects their subsequent communication with ECs is unknown. The vasculogenic characteristics of human ASCs in progenitor stage and after differentiation towards SMC phenotype were analysed in the present study. Exposure to transforming growth factor β1 (TGFβ1) or activin A has induced expression of SMC markers in ASCs. Analysis ...
BioAssay record AID 44634 submitted by ChEMBL: HSF produced by bone marrow-derived stromal cell lines C6.4 on stimulation with the compound at (1000 ng/mL) was determined in vitro in an GM-CFC assay..
Expression of uPAR in tumor-associated stromal cells is associated with colorectal cancer patient prognosis: a TMA study. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
TY - JOUR. T1 - Intracerebral Xenotransplantation of GFP Mouse Bone Marrow Stromal Cells in Intact and Stroke Rat Brain. T2 - Graft Survival and Immunologic Response. AU - Irons, H.. AU - Lind, J. G.. AU - Wakade, Chandramohan G.. AU - Yu, G.. AU - Hadman, M.. AU - Carroll, James Edwin. AU - Hess, David C. AU - Borlongan, Cesar V.. PY - 2004/1/1. Y1 - 2004/1/1. N2 - The present study characterized survival and immunologic response of bone marrow stromal cells (BMSCs) following transplantation into intact and stroke brains. In the first study, intrastriatal transplantation of BMSC (60,000 in 3 μl) or vehicle was performed in normal adult Sprague-Dawley male rats that subsequently received daily cyclosporin A (CsA, 10 mg/kg, IP in 3 ml) or vehicle (olive oil, similar volume) starting on day of surgery up to 3 days posttransplantation. Animals were euthanized at 3 or 30 days posttransplantation and brains were processed either for green fluorescent protein (GFP) microscopy or flow cytometry ...
Bone marrow stromal cells protect hematopoietic cells and provide drug resistance by delivering bunch of variable proteins. Thus, alterations of protein expression are typically associated with cell-cell signal transduction and regulation of cellular functions. Co-culture models of bone marrow stromal cells and hematopoietic cells are often used in studies of their crosstalk. Studies of altered protein expression initiated by stromal cell/hematopoietic cell interactions are an important new trend in microenvironmental research. There has been no report to date of global quantitative proteomics analysis of crosstalk between hematopoietic cells and stromal cells. In this study, we analyzed quantitative proteomes in a co-culture system of stromal HS5 cells and hematopoietic KG1a cells, and simultaneously tracked differentially expressed proteins in two types of cells before and after co-culture by stable isotope labeling by amino acids in cell culture (SILAC) method. We have shown that in co-cultured KG1a,
Non-healing bone fractures and periodontal bone loss constitute significant clinical problems with few approved medical options. Bone repair is enhanced by the presence of osteoblasts or osteoblastic precursor cells. Subcutaneous adipose tissue is a plentiful, accessible, and replenishable source of human stromal cells for transplantation. In Phase I of this SBIR, we tested the hypothesis that human adipose tissue-derived stromal cells are capable of osteoblast function. Substantial in vitro data indicates that these stromal cells differentiate into cells biochemically and morphologically similar to osteoblasts. The ability of these cells to form bone in vivo was examined as well. Phase II of this SBIR will extend these in vivo experiments. Specific Aim 1 examines the ability of human adipose tissue-derived stromal cells to form ectopic bone in hydroxyapatite ceramic cubes implanted subcutaneously in immunodeficient mice. Specific Aim 2 explores whether the introduction of a modified bone ...
In this study, we analyzed the influence of mesenchymal stromal cells derived from lymph nodes of non-Hodgkin lymphomas, on effector functions and differentiation of Vδ2 T lymphocytes. We show that: i) lymph-node mesenchymal stromal cells of non-Hodgkin lymphoma inhibit NKG2D-mediated lymphoid cell killing, but not rituximab-induced antibody-dependent cell-mediated cytotoxicity, exerted by Vδ2 T lymphocytes; ii) pre-treatment of mesenchymal stromal cells with the aminobisphosphonates pamidronate or zoledronate can rescue lymphoma cell killing via NKG2D; iii) this is due to inhibition of transforming growth factor-β and increase in interleukin-15 production by mesenchymal stromal cells; iv) aminobiphosphonate-treated mesenchymal stromal cells drive Vδ2 T lymphocyte differentiation into effector memory T cells, expressing the Thelper1 cytokines tumor necrosis factor-α and interferon-γ. In non-Hodgkin lymphoma lymph-nodes, Vδ2 T cells were mostly naive; upon co-culture with autologous ...
In this study, the role of histamine in interleukin-1 (IL-1) formation in murine bone marrow stromal cells was investigated in vitro. It was found that histamine and 4-methylhistamine increased the number of granulocyte colony-forming units in murine bone marrow cells. A similar effect was elicited by dibutyryl-cAMP and theophylline. When histamine and H2 agonists, such as 4-methylhistamine and dimaprit, were added to the culture medium containing murine bone marrow stromal cells, thymocyte comitogenic activity detected in the medium increased significantly. However, no such effect was observed in the case of 2-methyl-histamine, an H1 agonist. Histamine-induced production of thymocyte comitogenic activity in bone marrow stromal cells was inhibited by some H2 antagonists, such as cimetidine, ranitidine, and famotidine, but not by the H1 antagonist pyrilamine. Histamine was also effective in inducing the colony-promoting activity in murine bone marrow stromal cells. This was also inhibited by H2 ...
Objectives: Human bone marrow stromal cells (hBMSCs) are adherent fibroblast-like cells found in the bone marrow. They are a heterogeneous population of cells that includes a subset of osteoprogenitors. BMSCs have been widely used for tissue engineering, especially for bone regeneration. However, for clinical application currently, large quantities of hBMSCs are usually required for transplantation which is typically produced by serial passages of the cells ex vivo. We examined the effects of in vitro expansion on hBMSCs proliferation, multidifferentiation, and gene expression profiles. Methods: hBMSCs were harvested from surgical waste bone specimens from 3 healthy adults with IRB approval. The hBMSCs were cultured in α-MEM with 10% FBS and 1% penicillin-streptomycin. hBMSCs were trypsinized and passaged when they reached 70-80% confluence. Cells from early passage (p2 or 3) were compared with late passage (p7 or 8). MTT assay was used to determine the growth kinetics of hBMSCs. ...
1α,25-Dihydroxyvitamin D3 [1,25(OH)2D3] is known to modulate Ca2+ metabolism in several cell types. Vitamin-D-dependent calcium binding proteins such as calbindin-D28K (28 kDa calcium binding proteins) have been shown to be regulated by 1,25(OH)2D3 but the mechanisms controlling calbindin synthesis are still poorly understood in human osteoblast cell culture models. The human bone marrow stromal cells (HBMSC) described in this paper developed a calcified matrix, expressed osteocalcin (OC), osteopontin (OP) and responded to 1,25(OH)2D3. The expression of vitamin D receptor mRNA was demonstrated by reverse transcription-PCR. Calbindin-D28K protein was identified only in cells arising from the sixth subculture, which exhibited a calcified matrix and all of the osteoblastic markers, e.g. OC and OP. It was demonstrated by dot-immunodetection using immunological probes, and by in situ hybridization using labelled cDNA probes. Moreover, vitamin D3 enhanced calbindin-D28K synthesis as well as OC ...
Systemic autoimmunity can be present years before clinical onset of rheumatoid arthritis (RA). Adaptive immunity is initiated in lymphoid tissue where lymph node stromal cells (LNSCs) regulate immune responses through their intimate connection with leucocytes. We postulate that malfunctioning of LNSCs creates a microenvironment in which normal immune responses are not properly controlled, possibly leading to autoimmune disease. In this study we established an experimental model for studying the functional capacities of human LNSCs during RA development. Twenty-four patients with RA, 23 individuals positive for autoantibodies but without clinical disease (RA risk group) and 14 seronegative healthy control subjects underwent ultrasound-guided inguinal lymph node (LN) biopsy. Human LNSCs were isolated and expanded in vitro for functional analyses. In analogous co-cultures consisting of LNSCs and peripheral blood mononuclear cells, αCD3/αCD28-induced T-cell proliferation was measured using
Connexin43 (Cx43) is a component of gap junctions and is involved in intercel- lular signaling following injury to tissues. The carboxyl terminus of Cx43 binds to the PDZ2 domain of ZO-1 in order to form gap junction plaques and connect to the cytoskeleton. A biomimetic peptide known as αCT-1, replicating the last 9 amino acids found in the carboxyl terminus of CX43, has been shown to improve wound healing by preferentially binding to the PDZ2 domain of ZO-1. A possible mecha- nism for its action is through the Epithelial-Mesenchymal Transformation (EMT). Scratch assays were performed on rat bone marrow stromal cells treated with the peptide and were then analyzed using qPCR, western blotting, confocal microscopy, and live cell imaging. The gene expression analysis showed up-regulation of F11r and Krt19 and down-regulation of Mmp3. Protein expression analysis indicated an increase in Krt19 and the complete absence of Snai2 in the αCT-1 treated samples. Confocal microscopy suggested increased actin
Autologous adipose tissue-derived stromal vascular fraction cells application in patients with osteoarthritis Cognizant Communication Corporation Source Cognizant Communication Corporation DOI: 10.3727/096368915X686760 Jaroslav Michalek1*, Rene Moster2, Ladislav Lukac3, Kenneth Proefrock4, Miron Petrasovic5, Jakub Rybar5, Martina Capkova6, Ales Chaloupka7, Adas Darinskas8, Jaroslav Michalek, sr.9, Jan Kristek10, Jan Travnik11, Petr Jabandziev12, Marek…
TY - JOUR. T1 - Locally delivered growth factor enhances the angiogenic efficacy of adipose-derived stromal cells transplanted to ischemic limbs. AU - Bhang, Suk Ho. AU - Cho, Seung Woo. AU - Lim, Jae Min. AU - Kang, Jin Muk. AU - Lee, Tae Jin. AU - Yang, Hee Seok. AU - Song, Young Soo. AU - Park, Moon Hyang. AU - Kim, Hyo Soo. AU - Yoo, Kyung Jong. AU - Jang, Yangsoo. AU - Langer, Robert. AU - Anderson, Daniel G.. AU - Kim, Byung Soo. PY - 2009/8/1. Y1 - 2009/8/1. N2 - Ischemia is a potentially fatal medical event that is associated with as many as 30% of all deaths. Stem cell therapy offers significant therapeutic promise, but poor survival following transplantation to ischemic tissue limits its efficacy. Here we demonstrate that nanosphere-mediated growth factor delivery can enhance the survival of transplanted human adipose-derived stromal cells (hADSCs) and secretion of human angiogenic growth factors per cell, and substantially improve therapeutic efficacy of hADSCs. In vitro, in hypoxic ...
Non-hematopoietic stromal cells play important roles in many tissues, constructing tissue microenvironments, contributing to tissue repair, defense and immune responses. Within lymphoid organs, stromal cells organize and interact with leukocytes in an immunologically important manner. In addition to organizing T and B cell segregation and expressing lymphocyte survival factors, stromal cells support the migration and interactions between antigen presenting cells and naïve T and B cells during the initiation of immune responses and influence the outcome between tolerance and immunity. Stromal cells also play instrumental roles in coordinating immune responses in non-lymphoid tissues, in inflammatory and autoimmune diseases, and in chronic infection. For instance, stromal cell dysregulation has been seen in HIV infection and in cancer patients. Furthermore, stromal cells are being harnessed for therapeutic applications in several diseases, an area that holds great promise for improving human ...
Adipose/fat tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration and can also give rise to a substantial number of cultured, multipotent adipose-derived stromal cells (ADSCs). Recently, both SVF and ADSCs have gained wide-ranging translational significance in regenerative medicine. Initially used for cosmetic breast enhancement, this mode of treatment has found use in many diseases involving immune disorders, tissue degeneration, and ischaemic conditions. In this review, we try to address several important aspects of this field, outlining the biology, technology, translation, and challenges related to SVF- and ADSC-based therapies. Starting from the basics of SVF and ADSC isolation, we touch upon recently developed technologies, addressing elements of novel methods and devices under development for point-of-care isolation of SVF. Characterisation of SVF cells and ADSCs is also an evolving area and we look into unusual expression of CD34 antigen as an
The purpose of this study was to test the hypothesis that specific macrophage-secreted cytokines cause gene expression changes in endometrial stromal cells that reproduce the effects of macro-phages in the development of endometriosis. Telomerase-immortalized human endometrial stromal cells (T-HESC) were treated with tumor necrosis factor α (TNFα, 5 ng/ml) and interleukin 1β (IL1β, 1 ng/ml). Differential expression of 249 genes was identified by DNA microarray. Ontologies such as peptidases, cell adhesion, cell death/cell cycle, growth factors, cytoskeletal organization, defense/immune system, signal transduction, and transcriptional regulation which are related to the development of endometriosis were represented by these genes. The up-regulation of interleukin 8 (IL8), interleukin 6 (IL6), IL1β and matrix metallopro-teinase 3 (MMP3) in response to TNFα ± ILIβ in T-HESC cells was confirmed by real time RT-PCR. TNFα ± ILIβ did not affect the migration or invasion of T-HESC cells. This study
TY - JOUR. T1 - CD90+ stromal cells are the major source of IL-6, which supports cancer stem-like cells and inflammation in colorectal cancer. AU - Huynh, Phuong T.. AU - Beswick, Ellen J.. AU - Coronado, Yun A.. AU - Johnson, Paul. AU - OConnell, Malaney R.. AU - Watts, Tammara. AU - Singh, Pomila. AU - Qiu, Suimin. AU - Morris, Katherine. AU - Powell, Don W.. AU - Pinchuk, Irina V.. PY - 2016/4/15. Y1 - 2016/4/15. N2 - IL-6 is a pleiotropic cytokine increased in CRC and known to directly promote tumor growth. Colonic myofibroblasts/fibroblasts (CMFs or stromal cells) are CD90+ innate immune cells representing up to 30% of normal colonic mucosal lamina propria cells. They are expanded in CRC tumor stroma, where they also known as a cancer associated fibroblasts (CAFs). Cells of mesenchymal origin, such as normal myofibroblasts/fibroblasts, are known to secrete IL-6; however, their contribution to the increase in IL-6 in CRC and to tumor-promoting inflammation is not well defined. Using in ...
TY - JOUR. T1 - Brain from bone. T2 - Efficient "meta-differentiation" of marrow stroma-derived mature osteoblasts to neurons with Noggin or a demethylating agent. AU - Kohyama, Jun. AU - Abe, Hitoshi. AU - Shimazaki, Takuya. AU - Koizumi, Amane. AU - Nakashima, Kinichi. AU - Gojo, Satoshi. AU - Taga, Tetsuya. AU - Okano, Hideyuki. AU - Hata, Jun Ichi. AU - Umezawa, Akihiro. PY - 2001/10. Y1 - 2001/10. N2 - Bone marrow stromal cells are able to differentiate into adipogenic, chondrogenic, myogenic, osteogenic, and cardiomyogenic lineages, all of which are limited to a mesoderm-derived origin. In this study, we showed that neurons, which are of an ectoderm-origin, could be generated from marrow-derived stromal cells by specific inducers, fibronectin/ornithine coating, and neurosphere formation. The neurons generated from marrow stroma formed neurites, expressed neuron-specific markers and genes, and started to respond to depolarizing stimuli as functional mature neurons. Among stromal cells, ...
Stromal cells are connective tissue cells of any organ, for example in the uterine mucosa (endometrium), prostate, bone marrow, lymph node and the ovary. They are cells that support the function of the parenchymal cells of that organ. Fibroblasts and pericytes are among the most common types of stromal cells. The interaction between stromal cells and tumor cells is known to play a major role in cancer growth and progression. In addition, by regulating local cytokine networks (e.g. M-CSF, LIF), bone marrow stromal cells have been described to be involved in human haematopoiesis and inflammatory processes. Stromal cells (in the dermis layer) adjacent to the epidermis (the very top layer of the skin) release growth factors that promote cell division. This keeps the epidermis regenerating from the bottom while the top layer of cells on the epidermis are constantly being "sloughed" off the body. Certain types of skin cancers (basal cell carcinomas) cannot spread throughout the body because the cancer ...
Hormone-regulated proliferation and differentiation of endometrial stromal cells (ESCs) determine overall endometrial plasticity and receptivity to embryos. Previously we revealed that ESCs may undergo premature senescence, accompanied by proliferation loss and various intracellular alterations. Here we focused on whether and how senescence may be transmitted within the ESCs population. We revealed that senescent ESCs may induce paracrine senescence in young counterparts via cell contacts, secreted factors and extracellular vesicles. According to secretome-wide profiling we identified plasminogen activator inhibitor -1 (PAI-1) to be the most prominent protein secreted by senescent ESCs (data are available via ProteomeXchange with identifier PXD015742). By applying CRISPR/Cas9 techniques we disclosed that PAI-1 secreted by senescent ESCs may serve as the master-regulator of paracrine senescence progression within the ESCs population. Unraveled molecular basis of senescence transduction in the ESCs
It has been widely accepted that tumor cells and normal stromal cells in the host environment coordinately modulate tumor progression. Mitogen-activated protein kinase pathways are the representative stress-responsive cascades that exert proper cellular responses to divergent environmental stimuli. Genetically engineered mouse models and chemically induced tumorigenesis models have revealed that components of the MAPK pathway not only regulate the behavior of tumor cells themselves but also that of surrounding normal stromal cells in the host environment during cancer pathogenesis. The individual functions of MAPK pathway components in tumor initiation and progression vary depending on the stimuli and the stromal cell types involved in tumor progression, in addition to the molecular isoforms of the components and the origins of the tumor. Recent studies have indicated that MAPK pathway components synergize with environmental factors (e.g. tobacco smoke and diet) to affect tumor initiation and
In vitro osteogenic potential of human bone marrow stromal cells cultivated in porous scaffolds from mineralized collagen.: Porous 3D structures from mineralize
Physical interaction between human lymphomas and murine bone marrow derived stromal cells were studied. Nalm-6 pre-B cells adhered to BMS2 stromal cells and subsequently migrated beneath them, while Ramos Burkitt lymphoma cells, adhered but did not migrate. Four mAbs were established against Nalm-6 cells, which were able to block initial adhesion of Nalm-6 cells. Two of them were directed against the alpha 4 chain of VLA-4, and other two recognized the beta 1 chain of VLA integrins. Therefore, the initial adhesion of Ramos and Nalm-6 cells to BMS2 was largely mediated by the VLA-4 integrin expressed on lymphocytes. The corresponding ligand on stromal cells appears to be VCAM-1, because antibodies against murine VCAM-1 blocked the adhesion. However, antibodies against the alpha chain of VLA-4 were not capable of blocking subsequent migration beneath stromal cells. In contrast, antibodies against the beta chain of VLA integrins blocked the migration beneath stromal cells as well as the initial ...
TY - JOUR. T1 - TGF-β signaling in stromal cells acts upstream of FGF-10 to regulate epithelial stem cell growth in the adult lung. AU - McQualter, Jonathan L.. AU - McCarty, Rosa C.. AU - Van der Velden, Joanne. AU - ODonoghue, Robert J.J.. AU - Labat, Marie-Liesse. AU - Bozinovski, Steven. AU - Bertoncello, Ivan. PY - 2013/11/1. Y1 - 2013/11/1. N2 - Tissue resident mesenchymal stromal cells (MSCs) contribute to tissue regeneration through various mechanisms, including the secretion of trophic factors that act directly on epithelial stem cells to promote epithelialization. However, MSCs in tissues constitute a heterogeneous population of stromal cells and different subtypes may have different functions. In this study we show that CD166neg and CD166pos lung stromal cells have different proliferative and differentiative potential. CD166neg lung stromal cells exhibit high proliferative potential with the capacity to differentiate along the lipofibroblastic and myofibroblastic lineages, whereas ...
Results WT mice stromal cells display early acquisition of lymphoid features, up-regulating gp38 already at 3hours pc. Peak of this activation was observed at day5 with significant increase in the percentage of gp38+ lymphoid like stromal cells (LLSc), this increase is maintained between day8-15 pc. However, full acquisition of lymphoid phenotype, indicated by high levels of lymphoid CKs/cytokine expression was observed by day8-15 pc. This correlates with full maturation of the lymphoid aggregates in terms of T/B cell segregation and FDC formation. Of interest, RAG and LTbR KO mice showed normal degree of stromal cell activation in the early phases (2-5days p.c.) but a dramatic decrease in the percentage of LLSc by day15. However, these KOs exhibited a defect in full lymphoid conversion of stromal cells shown by significant decrease in CKs/cytokine expression and aggregates disorganization, suggesting that the full maturation of stromal cells require lymphocyte-derived signals such as ...
Finally, the mechanism by which p202 activates BMSC osteogenesis was determined. Runx2 is a critical transcription factor in osteogenesis. Previous study has detected the association of p204 with Runx2 [18]. However, in the present study, no interaction of p202 with Runx2 was seen (Figure 5C). p202 may lack an interacting structure with Runx2 protein. Id proteins are important suppressors in the differentiation of many cell types [13,20,21]. We found that Id proteins not only bound to Runx2, but also associated with p202 in the course of BMSC osteogenesis, and Id2 was a major associated family member (Figure 5A,B). It is possible that p202 disturbs the formation of Runx2/Ids complex and frees Runx2 to induce the differentiation process. Subsequent investigation demonstrated that this is the case. SiRNA-p202 dramatically lowered the p202-bound Id2, while enhanced the Runx2-associated Id2 content significantly. However, p202 overexpression increased the p202-bound Id2, but decreased the ...
Background: Improved understanding of the interactions between bone cells and endothelial cells involved in osteogenesis should aid the development of new strategies for bone tissue engineering. The aim of the present study was to determine whether direct communication between bone marrow stromal cells (MSC) and human umbilical vein endothelial cells (EC) could influence the osteogenic potential of MSC in osteogenic factor-free medium. Methods: After adding EC to MSC in a direct-contact system, cell viability and morphology were investigated with the WST assay and immnostaining. The effects on osteogenic differentiation of adding EC to MSC was systematically tested by the using Superarray assay and results were confirmed with real-time PCR. Results: Five days after the addition of EC to MSC in a ratio of 1:5 (EC/MSC) significant increases in cell proliferation and cellular bridges between the two cell types were detected, as well as increased mRNA expression of alkaline phosphatase (ALP). This ...
Bone marrow stromal cells (BMSCs) constitute a cell population routinely used as a representation of mesenchymal stem cells in vitro. They reside within the bone marrow cavity alongside hematopoietic stem cells (HSCs), which can give rise to red blood cells, immune progenitors, and osteoclasts. Thus, extractions of cell populations from the bone marrow results in a very heterogeneous mix of various cell populations, which can present challenges in experimental design and confound data interpretation. Several isolation and culture techniques have been developed in laboratories in order to obtain more or less homogeneous populations of BMSCs and HSCs invitro. Here, we present two methods for isolation of BMSCs and HSCs from mouse long bones: one method that yields a mixed population of BMSCs and HSCs and one method that attempts to separate the two cell populations based on adherence. Both methods provide cells suitable for osteogenic and adipogenic differentiation experiments as well as functional assays
Petrakis, S., Raskó, T., Mátés, L., Ivics, Z., Izsvák, Z., Kouzi-Koliakou, K. and Koliakos, G. (2012), Gateway-compatible transposon vector to genetically modify human embryonic kidney and adipose-derived stromal cells. Biotechnology Journal, 7: 891-897. doi: 10.1002/biot.201100471 ...
Cell transplantation using bone marrow stromal cells (BMSCs) to alleviate neurological deficits has recently become the focus of research in regenerative medicine. Evidence suggests that secretion of various growth-promoting substances likely plays an important role in functional recovery against neurological diseases. In an attempt to identify a possible mechanism underlying the regenerative potential of BMSCs, this study investigated the production and possible contribution of neurotrophic factors by transected sciatic nerve defect in a rat model with a 15 mm gap. Cultured BMSCs became morphologically homogeneous with fibroblast-like shape after ex vivo expansion. We provided several pieces of evidence for the beneficial effects of implanted fibroblast-like BMSCs on sciatic nerve regeneration. When compared to silicone tube control animals, this treatment led to (i) improved walking behavior as measured by footprint analysis, (ii) reduced loss of gastrocnemius muscle weight and EMG magnitude, ...
Mammalian hematopoietic stem cell (HSC) commitment and differentiation into lymphoid lineage cells proceed through a series of developmentally restricted progenitor compartments. A complete understanding of this process, and how it differs from HSC commitment and differentiation into cells of the myeloid/erythroid lineages, requires the development of model systems that support HSC commitment to the lymphoid lineages. We now describe a human bone marrow stromal cell culture that preferentially supports commitment and differentiation of human HSC to CD19+ B-lineage cells. Fluorescence activated cell sorterpurified CD34++/lineage-cells were isolated from fetal bone marrow and cultured on human fetal bone marrow stromal cells in serum-free conditions containing no exogenous cytokines. Over a period of 3 weeks, CD34++/lineage- cells underwent commitment, differentiation, and expansion into the B lineage. Progressive changes included: loss of CD34, acquisition of and graded increases in the level of ...
Surface epithelial-stromal tumors are a class of ovarian neoplasms that may be benign or malignant. Neoplasms in this group are thought to be derived from the ovarian surface epithelium (modified peritoneum) or from ectopic endometrial or Fallopian tube (tubal) tissue. This group of tumors accounts for 90% to 95% of all cases of ovarian cancer.[1][2] The pathogenesis of surface epithelial-stromal tumor is characterized by the overgrowth of the ovarian surface epithelium. Common risk factors in the development of surface epithelial-stromal tumor, include: nulliparity, early menopause, gonadal dysgenesis, family history (e.g. BRCA1/BRCA2 mutations), smoking, previous history of breast, and endometrial or colon cancer (Lynch II). The prevalence of surface epithelial-stromal tumor is approximately 3 per 100,000 individuals worldwide. Surface epithelial-stromal tumor is more commonly observed among postmenopausal women. Early clinical features of surface epithelial-stromal tumor include pelvic ...
Cleveland Clinic Background: Nerve gaps that need conduit or allograft material tend to involve regional loss of overlying soft tissues and muscles spanning a gap of over 20 cm. Currently used autograft technique requires immunosuppression and demonstrated poor motor recovery.. Main goals: We developed epineural sheath conduit supported with bone marrow stromal cells (BMSCs) to restore 6 cm nerve defects. Epineural sheath is immunologically neutral and contains laminin, enhancing neuronal growth. Addition of BMSCs will contribute to structural support and secretion of growth factors for nerve regeneration.. Methods: Sheep model was used since sheep peripheral nerves are histologically and morphometrically similar to human nerves. Epineural sheath tube was created from the median nerve by the pull out technique, removing all fascicles. BMSCs were obtained from donor animal, purified by the buffy coat technique and cultured for 14 days. Next, cells were fluorescently labeled and injected into the ...
The activated tumor stroma participates in many processes that control tumorigenesis, including tumor cell growth, invasion and metastasis. Cancer-associated fibroblasts (CAFs) represent the major cellular component of the stroma and are the main source for connective tissue components of the extracellular matrix and various classes of proteolytic enzymes. The signaling pathways involved in the interactions between tumor and stromal cells and the molecular characteristics that distinguish normal resting fibroblasts from cancer-associated or -activated fibroblasts remain poorly defined. Recent studies emphasized the prognostic and therapeutic significance of CAF-related molecular signatures and a number of those genes have been shown to serve as putative therapeutic targets. We have used immuno-laser capture microdissection and whole-genome Affymetrix GeneChip analysis to obtain transcriptional signatures from the activated tumor stroma of colon carcinomas that were compared with normal resting
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Among cells present in the tumor microenvironment, activated fibroblasts termed cancer-associated fibroblasts (CAFs), play a critical role in the complex process of tumor-stroma interaction. CAFs, one of the prominent stromal cell populations in most types of human carcinomas, have been involved in tumor growth, angiogenesis, cancer stemness, extracellular matrix remodeling, tissue invasion, metastasis and even chemoresistance. During the past decade, these activated tumor-associated fibroblasts have also been involved in the modulation of the anti-tumor immune response on various levels. In this review, we describe our current understanding of how CAFs accomplish this task as well as their potential therapeutic implications.
The bone marrow microenvironment influences malignant hematopoiesis, but how it promotes leukemogenesis has not been elucidated. In addition, the role of the bone marrow stroma in regulating clinical responses to DNA methyltransferase inhibitors (DNMTi) is also poorly understood. In this study, we conducted a DNA methylome analysis of bone marrow-derived stromal cells from myelodysplastic syndrome (MDS) patients and observed widespread aberrant cytosine hypermethylation occurring preferentially outside CpG islands. Stroma derived from 5-azacytidine-treated patients lacked aberrant methylation and DNMTi treatment of primary MDS stroma enhanced its ability to support erythroid differentiation. An integrative expression analysis revealed that the WNT pathway antagonist FRZB was aberrantly hypermethylated and underexpressed in MDS stroma. This result was confirmed in an independent set of sorted, primary MDS-derived mesenchymal cells. We documented a WNT/β-catenin activation signature in CD34+ cells from
The bone marrow microenvironment influences malignant hematopoiesis but how it promotes leukemogenesis has not been elucidated. Additionally, the role of the bone marrow stroma in regulating clinical responses to DNA methyltransferase inhibitors (DNMTi) is also poorly understood. In this study, we conducted a DNA methylome analysis of bone marrow-derived stromal cells from myelodysplastic syndrome (MDS) patients and observed widespread aberrant cytosine hypermethylation occurring preferentially outside CpG islands. Stroma derived from 5-azacytidine-treated patients lacked aberrant methylation and DNMTi treatment of primary MDS stroma enhanced its ability to support erythroid differentiation. An integrative expression analysis revealed that the WNT pathway antagonist FRZB was aberrantly hypermethylated and underexpressed in MDS stroma. This result was confirmed in an independent set of sorted, primary MDS-derived mesenchymal cells. We documented a WNT/ß-catenin activation signature in CD34+ ...
In this study, we characterized signaling cascades activated on direct contact of leukemic cells with bone marrow-derived stromal cells. Our findings indicate stroma-induced activation of ILK with phosphorylation of Akt and GSK3β. Because p-GSK3βSer9 is a known cellular target of ILK ( 7), these results are consistent with evidence of ILK activation induced by growth factors or engagement of the integrins by stroma. We further showed that the nuclear accumulation of β-catenin, a downstream GSK3β target, was induced in NB4 cells by coculture with MSCs and that this accumulation was abrogated when the PI3K/ILK pathway was inhibited. In contrast, the GSK3 inhibitor BIO, which prevented the serum withdrawal-induced apoptosis of NB4 cells, stimulated the translocation of β-catenin to the nucleus in cells growing without stromal support. Altogether, these findings provide evidence that stroma-induced ILK activation results in GSK3 inhibition and up-regulation of nuclear β-catenin consistent with ...
Li et al. used stable isotope labeling with amino acids in cell culture (SILAC) to analyze proteomic differences in HS5 and HS27a cells.
Health,Tampa FL (Feb. 13 2004) -- Bone marrow stromal cells release a blood...The laboratory study was published in the January 2004 issue of the jo... Were suggesting that transplanted bone marrow stromal cells may hast...Researchers at USF and other institutions have demonstrated that some ...However no one has proven that this recovery results from converted b...,Hormone,released,by,bone,marrow,cells,may,hasten,recovery,from,brain,injury,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
COLUMBUS, Ohio-New evidence suggests that the genetic changes leading to breast cancer occur first in the epithelium of breast tissue, and then are followed by corresponding alterations in the surrounding stroma. 1
TY - JOUR. T1 - Cyclooxygenase-2 inhibitor celecoxib augments chemotherapeutic drug-induced apoptosis by enhancing activation of caspase-3 and -9 in prostate cancer cells. AU - Dandekar, Devendra S.. AU - Lopez, Monica. AU - Carey, Robert I.. AU - Lokeshwar, Bal L.. PY - 2005/6/20. Y1 - 2005/6/20. N2 - Many tumors constitutively express high levels of the inducible form of proinflammatory enzyme, cyclooxygenase-2 (COX-2). Increased COX-2 expression is associated with tumor cell resistance to many cytotoxic chemotherapy drugs. Furthermore, increased resistance to cytotoxic antitumor drugs is also known to be dependent on associated stromal cells in many tumors. We investigated whether prostate tumor-associated stromal cells, marrow-derived osteoblasts, affect cytotoxicity of 2 antitumor drugs, COL-3 and docetaxel (TXTR), and whether it is dependent on COX-2 activity. We further examined whether inhibiting the activity of COX-2 negate the stroma-induced decrease in drug sensitivity in tumor cells, ...
TY - JOUR. T1 - Differential macrophage programming in the tumor microenvironment. AU - Ruffell, Brian. AU - Affara, Nesrine I.. AU - Coussens, Lisa M.. PY - 2012/3/1. Y1 - 2012/3/1. N2 - Of the multiple unique stromal cell types common to solid tumors, tumor-associated macrophages (TAMs) are significant for fostering tumor progression. The protumor properties of TAMs derive from regulation of angiogenic programming, production of soluble mediators that support proliferation, survival and invasion of malignant cells, and direct and indirect suppression of cytotoxic T cell activity. These varied activities are dependent on the polarization state of TAMs that is regulated in part by local concentrations of cytokines and chemokines, as well as varied interactions of TAMs with normal and degraded components of the extracellular matrix. Targeting molecular pathways regulating TAM polarization holds great promise for anticancer therapy.. AB - Of the multiple unique stromal cell types common to solid ...
TY - JOUR. T1 - Distinct cancer-promoting stromal gene expression depending on lung function. AU - Sandri, Brian J.. AU - Masvidal, Laia. AU - Murie, Carl. AU - Bartish, Margarita. AU - Avdulov, Svetlana. AU - Higgins, Lee Ann. AU - Markowski, Todd. AU - Peterson, Mark. AU - Bergh, Jonas. AU - Yang, Ping. AU - Rolny, Charlotte. AU - Limper, Andrew H.. AU - Griffin, Timothy J.. AU - Bitterman, Peter B.. AU - Wendt, Chris H.. AU - Larsson, Ola. PY - 2019/8/1. Y1 - 2019/8/1. N2 - Rationale: Chronic obstructive pulmonary disease is an independent risk factor for lung cancer, but the underlying molecular mechanisms are unknown. We hypothesized that lung stromal cells activate pathological gene expression programs that support oncogenesis. Objectives: To identify molecular mechanisms operating in the lung stroma that support the development of lung cancer. Methods: The study included subjects with and without lung cancer across a spectrum of lung-function values. We conducted a multiomics analysis of ...
Dying endometrial stromal cells - posted in Stem Cell: Hello to everyone! We do have a problem in our lab with endometrial stem cells. We are trying do decidualize them using hormones - Estrogen, Progesterone and cAMP. The problem is that cells are dying on 3 to 6 day. First we though it could be from the alcohol in which Estrogen and Progesterone are dissolved, but the control wells with just the same amount of alcohol seemed just fine. Then we though it could be from the NH4OH in...
Sigma-Aldrich offers abstracts and full-text articles by [Amy L Strong, Annie C Bowles, Connor P MacCrimmon, Trivia P Frazier, Stephen J Lee, Xiying Wu, Adam J Katz, Barbara Gawronska-Kozak, Bruce A Bunnell, Jeffrey M Gimble].
The progression of a lesion to a carcinoma is dependent on the engagement of reactive stroma that provides structural and vascular support for tumour growth and also leads to tissue reorganization and invasiveness. The composition of reactive stroma closely resembles that of granulation tissue, and myofibroblasts are thought to play a critical role in driving the stromal reaction of invasive tumours as well as of physiological wound repair. In the present work, we established a myofibroblast-like cell line, named A17, from a mouse mammary carcinoma model in which tumourigenesis is triggered in a single step by the overexpression of HER-2/neu transgene in the epithelial compartment of mammary glands. We showed that although they derived from a tumour of epithelial origin and did not express HER-2/neu transgene, their subcutaneous injection into the backs of syngeneic mice gave rise to sarcomatoid tumours which expressed alpha-smooth muscle actin at the invasive edge. The expression of ...