en] The activity of the D-alanyl-D carboxypeptidase from the penicillin-resistant Streptomyces albus G is not or very little affected by penicillins and related antibiotics. The molecular basis for the mechanism of action of penicillin is discussed. The Streptomyces albus G D-alanyl-D carboxypeptidase appears as a model for the study of a mechanism of penicillin resistance that does not involve the enzymatic degradation of the antibiotic. Vancomycin and ristocetin are shown to inhibit the hydrolysis of sensitive peptides by the Streptomyces albus G D-alanyl-D carboxypeptidase and the mechanism of inhibition is discussed ...
Streptomyces thermoviolaceus ChiS protein: similar to histidine kinases; a member of a two-component sensor-regulator system; isolated from Streptomyces thermoviolaceus; amino acid sequence in first source; GenBank AB016841
Streptomyces isolates have yielded the majority of human, animal, and agricultural antibiotics, as well as a number of fundamental chemotherapy medicines. Streptomyces is the largest antibiotic-producing genus of actinobacteria, producing chemotherapy, antibacterial, antifungal, antiparasitic drugs, and immunosuppressants. Streptomyces isolates are typically initiated with the aerial hyphal formation from the mycelium. Streptomyces, yielded the medicines doxorubicin (Doxil), daunorubicin (DaunoXome), and streptozotocin (Zanosar). Doxorubicin is the precursor to valrubicin (Valstar), myocet, and pirarubicin. Daunorubicin is the precursor to idarubicin (Idamycin), epirubicin (Ellence), and zorubicin. Streptomyces is the original source of dactinomycin (Cosmegen), bleomycin (Blenoxane), pingyangmycin (Bleomycin A5), mitomycin C (Mutamycin), rebeccamycin, staurosporine (precursor to stauprimide and midostaurin), neothramycin, aclarubicin, tomaymycin, sibiromycin, and mazethramycin. Derivatives of ...
P,Following translocation, bacterial lipoproteins are lipidated by lipoprotein diacylglycerol transferase (Lgt) and cleaved of their signal peptides by lipoprotein signal peptidase (Lsp). In Gram-negative bacteria and mycobacteria, lipoproteins are further lipidated by lipoprotein N-acyl transferase (Lnt), to give triacylated lipoproteins. Streptomyces are unusual amongst Gram-positive bacteria because they export large numbers of lipoproteins via the twin arginine protein transport (Tat) pathway. Furthermore, some Streptomyces species encode two Lgt homologues and all Streptomyces species encode two homologues of Lnt. Here we characterize lipoprotein biogenesis in the plant pathogen Streptomyces scabies and report that lgt and lsp mutants are defective in growth and development while only moderately affected in virulence. Lipoproteins are lost from the membrane in an S. scabies lgt mutant but restored by expression of Streptomyces coelicolor lgt1 or lgt2 confirming that both encode functional ...
TY - JOUR. T1 - Efficient Nε-lauroyl-l-lysine production by recombinant ε-lysine acylase from Streptomyces mobaraensis. AU - Koreishi, Mayuko. AU - Kawasaki, Ryoko. AU - Imanaka, Hiroyuki. AU - Imamura, Koreyoshi. AU - Takakura, Yasuaki. AU - Nakanishi, Kazuhiro. PY - 2009/5/20. Y1 - 2009/5/20. N2 - ε-Lysine acylase from Streptomyces mobaraensis (Sm-ELA), which specifically catalyzes hydrolysis of the ε-amide bond in various Nε-acyl-l-lysines, was cloned and sequenced. The Sm-ELA gene consists of a 1617-bp open reading frame that encodes a 538-amino acid protein with a molecular mass of 55,816 Da. An NCBI protein-protein BLAST search revealed that the enzyme belongs to the YtcJ-like metal-dependent amidohydrolase family, which is further characterized as the metallo-dependent hydrolase superfamily. The Sm-ELA gene was ligated into a pUC702 vector for expression in Streptomyces lividans TK24. Expression of recombinant Sm-ELA in S. lividans was approximately 300-fold higher than that in ...
Bagramyan, K., et al. Mass Spectrometric Resurrection of SHA, an LRhamnose and Beta-D-Galactose Binding Lectin from the Lost Strain Streptomyces 27S5. ASMS. 0, ThP 307. 01/06/2015.. ...
Clavulanic acid. Its not an antibiotic in the classical sense of the word, but where would be without it? Probably one of the most important discoveries in the history of antibiotic development.. Clavulanic acid, or potassium clavulanate, was discovered in the early 1970s by scientists at Beecham pharmaceuticals. Produced naturally by the bacterium Streptomyces clavuligerus, it is a potent inhibitor of (Class A) beta-lactamases.. We know that Streptomyces are soil bacteria and there are hundreds of different Streptomyces species recorded in the taxonomy. A majority of Streptomyces species produce beta-lactam antibiotics and a lot of them also produce beta-lactamases, showing the lengths that these bacteria would go to to survive amongst other streptomyces and also other micro-organisms living in close proximity. It is interesting therefore that one of the Streptomyces species (Streptomyces clavuligerus) had the brains to produce a beta-lactamase inhibitor. Why? This would have given the ...
Mangrove Streptomyces represent a rich source of novel bioactive compounds in medicinal research. A novel alkaloid, named 1-N-methyl-3-methylamino-[N-butanoic acid-3¢-(9¢-methyl-8¢-propen-7¢-one)-amide]-benzo[f][1,7]naphthyridine-2-one (1) was isolated from Streptomyces albogriseolus originating from mangrove sediments. The structure of compound 1 was elucidated by extensive spectroscopic data analyses and verified by the 13C-NMR calculation at the B3LYP/6-311+G(2d,p) level of theory.
Genes encoding two ribonucleotide reductases (RNRs) were identified in members of the genus Streptomyces. One gene, nrdJ, encoded an oligomeric protein comprising four identical subunits each with a molecular mass of ∼108 kDa. The activity of this protein depended on the presence of 5′-deoxyadenosylcobalamine (coenzyme B12), establishing it as a class II RNR. The Streptomyces clavuligerus nrdJ gene was cloned, using internal peptide sequences from the purified protein, and was found to encode a polypeptide of 961 aa. Molecular phylogenetic analysis showed that the S. clavuligerus class II RNR shares significant similarity with most other bacterial and archaeal class II RNRs. Two other genes, nrdA and nrdB, were initially identified in the Streptomyces coelicolor genome database in unannotated ORFs as encoding a class Ia RNR. Southern analysis demonstrated that the nrdAB genes were present in different Streptomyces spp. The S. coelicolor nrdAB genes were cloned and expressed in Escherichia coli, and
ID H2K393_STRHJ Unreviewed; 512 AA. AC H2K393; DT 21-MAR-2012, integrated into UniProtKB/TrEMBL. DT 21-MAR-2012, sequence version 1. DT 22-NOV-2017, entry version 39. DE RecName: Full=Probable DNA ligase {ECO:0000256,HAMAP-Rule:MF_00407}; DE EC=6.5.1.1 {ECO:0000256,HAMAP-Rule:MF_00407}; DE AltName: Full=Polydeoxyribonucleotide synthase [ATP] {ECO:0000256,HAMAP-Rule:MF_00407}; GN Name=lig {ECO:0000256,HAMAP-Rule:MF_00407}; GN OrderedLocusNames=SHJG_2641 {ECO:0000313,EMBL:AEY87915.1}; OS Streptomyces hygroscopicus subsp. jinggangensis (strain 5008). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=1133850 {ECO:0000313,EMBL:AEY87915.1, ECO:0000313,Proteomes:UP000007170}; RN [1] {ECO:0000313,Proteomes:UP000007170} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=5008 {ECO:0000313,Proteomes:UP000007170}; RA Wu H., Bai L.; RT Genomic analysis of Streptomyces hygroscopicus subsp. jinggangensis RT 5008.; RL Submitted (JAN-2012) to the ...
The reactions of beta-lactamases of Actinomadura R39 and Streptomyces albus G with clavulanate proceed along branched pathways. Both enzymes perform the hydrolysis of this beta-lactam with rather high efficiencies (kcat. = 18s-1 and 52s-1 respectively). If large clavulanate/enzyme ratios are used, complete inactivation of the enzymes is observed. At lower ratios, inactivation is only partial. Irreversible inactivation occurs after 400 and 20000 turnovers for the A. R39 and S. albus G enzymes respectively. With the A. R39 beta-lactamase, a transiently inhibited complex is also formed that remains undetectable with the S. albus G beta-lactamase. Kinetic models are presented and studied for the interaction between clavulanate and both enzymes. A tentative general reaction scheme is also discussed.. ...
SUMMARY: Germinating spores of Streptomyces viridochromogenes excreted a substance into the surrounding medium which inhibited germination of another sample of the spores. The germination inhibitor (GI) was produced during submerged culture after exponential growth had ceased. The GI was purified 51-fold following extraction from growth liquor with chloroform. It was soluble in alcohol and water and had a molecular weight of less than 1000. The GI blocked growth and respiration of some Gram-positive bacteria and was an inhibitor of the membrane bound, but not solubilized, calcium-dependent ATPase of germinated spores and mycelia of the producing organism. Several sodium-potassium activated ATPases were also inhibited. All four activities (respiration, growth, germination inhibition. ATPase) co-purified during column and thin-layer chromatography. The GI activities released during germination and produced during growth were identical. A role for the GI antibiotic in regulation of dormancy of spores of
Streptomyces violaceoruber ATCC ® BAA-471™ Designation: John Innes Centre M145 TypeStrain=False Application: The parental strain, Streptomyces coelicolor A3(2), was used worldwide in genetic studies.
Coloured scanning electron micrograph (SEM) of Streptomyces hygroscopicus, Gram-positive, aerobic, filamentous, biofilm-forming, rod prokaryote (bacterium). Streptomyces sp. belong to the Actinomycetes group and are bacteria that share many characteristics with the fungi. They grow usually as filaments (chains of cells) and often branch to form a network of filaments (mycelium) in the soil. These soil bacteria are responsible for the musty odour of soil. This strain of Streptomyces hygroscopicus produces the antibiotic, milbemycin, which is used as an insecticide and also to control certain parasitic infections in animals. It is also a biofilm forming bacterium. Biofilms are primarily accumulations of bacteria in aqueous environments. They form when bacteria secrete slimy, mucilaginous materials that provide the microorganisms with a means of attachment to moist surfaces. Magnification: x1,600 when shortest axis printed at 25 - Stock Image C032/2295
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Streptomyces hygroscopicus 5008 has been used for the production of the antifungal validamycin/jinggangmycin for more than 40 years. A high yield of validamycin is achieved by culturing the strain at 37°C, rather than at 30°C for normal growth and sporulation. The mechanism(s) of its thermo-regulated biosynthesis was largely unknown. The 10,383,684-bp genome of strain 5008 was completely sequenced and composed of a linear chromosome, a 164.57-kb linear plasmid, and a 73.28-kb circular plasmid. Compared with other Streptomyces genomes, the chromosome of strain 5008 has a smaller core region and shorter terminal inverted repeats, encodes more α/β hydrolases, major facilitator superfamily transporters, and Mg2+/Mn2+-dependent regulatory phosphatases. Transcriptomic analysis revealed that the expression of 7.5% of coding sequences was increased at 37°C, including biosynthetic genes for validamycin and other three secondary metabolites. At 37°C, a glutamate dehydrogenase was transcriptionally up
Strains VIM M 10366(T), YIM M 10378(T) and YIM M 10400(T) were isolated from marine sediments collected from the Xisha Islands in the South China Sea. All three isolates were able to grow optimally at pH 7.0, 28-37 degrees C and 0-3% (w/v) NaCl. Comparison of 16S rRNA gene sequences showed that these strains are members of the genus Streptomyces, exhibiting moderately high 16S rRNA gene sequence similarities of 97.0-98.8% to members of the most closely related Streptomyces species. Morphological characteristics, physiological characteristics and compositions of whole-cell sugars and phospholipids are consistent with the diagnostic characteristics of the genus Streptomyces, but still allowed differentiation amongst the three strains and their neighbours. Based on 16S rRNA gene sequence analysis, DNA DNA relatedness, phenotypic characteristics and chemotaxonomic data, strains VIM M 10366(T), VIM M 10378(T) and VIM M 10400(T) were identified as members of three novel species of the genus ...
Read Properties of lanK-based regulatory circuit involved in landomycin biosynthesis in Streptomyces cyanogenus S136, Russian Journal of Genetics on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Taxonomy of the species Streptomyces bambergiensis Wallhäusser et al. 1966 (Approved Lists 1980) pro synon. Streptomyces prasinus Ettlinger et al. 1958 (Approved Lists 1980) emend. Labeda et al. 2016
The cancer-cell-cytotoxicity-guided fractionation of the acetone extracts of two cultured marine-derived Streptomyces strains belonging to the MAR4 group yielded six new napyradiomycins, compounds A-F (1-6), together with three known compounds, napyradiomycins B2-B4 (7-9). Napyradiomycins 1-4 are new members of the napyradiomycin C-type meroterpenoids, which possess a linear monoterpene bridge between C-7 and C-10a. Compound 4 has an additional tetrahydropyran ring fused to the phenol moiety. Compounds 5-9 are related to the napyradiomycin B-type meroterpenoids. The structures of all new compounds were assigned by interpretation of 1D and 2D NMR, MS, and other spectroscopic data. The relative configurations were assigned based upon interpretation of ROESY 2D NMR experiments. The cytotoxicity of 1-9 against the human colon carcinoma cell line HCT-116 and their antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA) are presented.. ...
Streptomyces violaceoruber ATCC ® BAA-471D-5™ Designation: Genomic DNA from M145 (ATCC BAA-471) TypeStrain=False Application:
Spectrophotometric determination of the ionophore compounds and mass spectrometry of the biosynthesis products revealed that the cells of Streptomyces chrysomallus subsp. macrotetrolidi during the lag phase permanently contained practically constant though insignificant (about 20 to 40 nmol/mg of protein) amounts of biosynthetic precursors of macrotetrolides, oligomers of nactinic acids. The oligomers of nactinic acids had antibiotic activity and in an amount of 2.5 micrograms/ml inhibited the growth of Bacillus mycoides. The oligomers of nactinic acids had ionophore properties and were highly labile with respect to inorganic cations. The presence of sodium in the extragent stabilized the calcium monomer, a trimer of nonactinic acid, and promoted washing off the substances of the nactinic nature from the cells. The cations of ammonia and possibly potassium stabilized the dimer and tetramer of nonactinic acid forming a more hydrophobic complex by comparison with the initial compounds.
Streptomyces peucetius DnrS protein: involved in daunorubicin biosynthesis in Streptomyces peucetius; encodes a glycosyltransferase; amino acid sequence given in first source; GenBank L47164
BLEOCIN™ Streptomyces verticillus, Sterile-Filtered, Aqueous Solution - CAS 55658-47-4 - Calbiochem CAS 55658-47-4 A unique antibiotic of the bleomycin family that is toxic to eukaryotic and prokaryotic cells. - Find MSDS or SDS, a COA, data sheets and more information.
Original publication: Krainsky A. Die Aktinomyceten und ihre Bedeutung in der Natur. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abteilung II 1914; 41:649-688. Nomenclatural status: not validly published Correct name: Streptomyces diastatochromogenes (Krainsky 1914) Waksman and Henrici 1948 (Approved Lists 1980) ...
FR-900520 and FR-900523, novel neutral macrolide immunosuppressants, were isolated from the cultured broth of Streptomyces hygroscopicus subsp. yakushimaensis No. 7238. Their molecular formulae were determined as C43H69NO12 and C42H67NO12, respectively. The compounds suppressed immune response in vitro. IC50 values of FR-900520 and FR-900523 for mouse mixed lymphocyte reaction were 0.55 nM and 1.6 nM, respectively. FR-900520, the major component, clearly prolonged skin allograft survival in rats.
TY - JOUR. T1 - 12T061A and 12T061C, two new julichrome family compounds, as radical scavengers from Streptomyces sp. AU - Komoda, Toshikazu. AU - Saeki, Naoko. AU - Koseki, Yoshitaka. AU - Kiyota, Hiromasa. PY - 2016/2/29. Y1 - 2016/2/29. N2 - We identified two new radical scavengers, 12T061A (1, C19H20O7) and 12T061C (2, C20H22O7), from a culture of the Streptomyces sp. Spectroscopic analysis indicated that these compounds are new julichrome family compounds. Compounds 1 and 2 showed radical-scavenging activity with an ED50 of 370 μM and 18 μM, respectively. Moreover, 1 showed tumor cell growth suppressive activity in HepG2 cells, (IC50: 3.6 μM); however, no suppressive activity was shown in 2 (IC50: , 100 μM).. AB - We identified two new radical scavengers, 12T061A (1, C19H20O7) and 12T061C (2, C20H22O7), from a culture of the Streptomyces sp. Spectroscopic analysis indicated that these compounds are new julichrome family compounds. Compounds 1 and 2 showed radical-scavenging activity ...
als Synonym von Streptomyces setonii identifiziert,[7] so dass es sich nun nach aktueller Systematik um Streptomyces setonii (Millard & Burr 1926) Waksman 1953 emend. Antibiotikum, Die Arten dieser Gattung sind grampositiv, aerob, Myzel-bildend, mehrzellig, bilden Sporen und haben einen hohen GC-Gehalt. Short characterization of Streptomyces chromofuscus 34-1. Es beinhaltet die meisten Gene aller bisher untersuchten Bakterien. The spores were elongated with smooth surface (Fig. X‐119‐6, which catalyzes the oxidative deamination of l ‐glutamate, has attracted increasing attention as a component of amperometric l ‐glutamate sensors used in the food industry and clinical biochemistry. Sie benötigen für ihr… Michael T. Madigan, John M. Martinko, Jack Parker: E. A. Bayer, T. Kulik, R. Adar, M. Wilchek: H. Hager, F. v. Bruchhausen, R. Batty, G. Wurm: H. Drautz, W. Keller-Schierlein, H. Zähner: internationalen Code der Nomenklatur von Bakterien, Leibniz Institut DSMZ - Deutsche Sammlung ...
11181344] An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57. (Antimicrob Agents Chemother. , 2001 ...
Name: Streptomyces halstedii (Waksman and Curtis 1916) Waksman and Henrici 1948 (Approved Lists 1980). Category: Species. Proposed as: comb. nov.. Basonym: Actinomyces halstedii Waksman and Curtis 1916 Etymology: hal.stedi.i. N.L. gen. masc. n. halstedii, of Halsted, named for Professor Halsted of Rutgers University Gender: masculine Type strain: ATCC 10897; ATCC 19770; BCRC 13680; CBS 508.68; CCRC 13680; CECT 3328; DSM 40068; HAMBI 993; IFO 12783; IMET 40322; JCM 4584; NBRC 12783; NCIB 9839; NCIMB 9839; NRRL B-1238; NRRL ISP-5068; RIA 1050; UNIQEM 156; VKM Ac-1768 See detailed strain information at ...
Macro-photograph of a colony of the soil bacterium Streptomyces lividans. Streptomyces species are natural producers of a number of antibiotics, including streptomycin. A member of the Actinomycetes group, they share many characteristics with the fungi. The bacteria grow in the soil as a branching network of filaments, known as a mycelium. They also produce aerial mycelium, from the tips of which develop chains of spores. When the spores are mature, they rupture and are dispersed on the wind. - Stock Image B240/0019
TY - JOUR. T1 - Roles of the signal peptide and mature domains in the secretion and maturation of the neutral metalloprotease from Streptomyces cacaoi. AU - Chang, Su Chih. AU - Su, Mei Han. AU - Wu Lee, Yan-Hwa. PY - 1997/1/1. Y1 - 1997/1/1. N2 - The neutral metalloprotease (Npr) of Streptomyces cacaoi is synthesized as a prepro-Npr precursor form consisting of a secretory signal peptide, a propeptide and the mature metalloprotease. The maturation of Npr occurs extracellularly via an autoproteolytic processing of the secreted pro-Npr. The integrity of the propeptide is essential for the formation of mature active Npr but not for its secretion. In this study we investigated whether the secretion and maturation of Npr require the integrity of its signal peptide region and mature protease domain. Five signal peptide mutants were generated, including the substitution mutations at the positively charged region (mutant 1R6LE), the central hydrophobic region (mutants GI19EL and G19N), the boundary of ...
High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherechia coli. Isolation and antifungal and antioomycete activities of aerugine produced by Pseudomonas fluorescens strain MM-B16
Sequence analysis of a 3.4-kb region Streptomyces peucetius daunorubicin (DNR) gene cluster established the presence of the dnrH and dnmT genes. In dnrH mutants, DNR production increased 8.5-fold, compared with that in the wild-type strain, while dnmT mutants accumulated epsilon-rhodomycinone (RHO), which normally becomes glycosylated in daunorubicin biosynthesis. Hence, dnmT may be involved in the biosynthesis or attachment of daunosamine to RHO or in the regulation of this process. Since the DnrH protein is similar to known glycosyl transferases, this protein may catalyze the conversion of DNR to its polyglycosylated forms, known as baumycins. Overexpression of dnmT in the wild-type and dnrH mutant strains resulted in a major decrease in RHO accumulation and increase in DNR production. ...
The genome of Streptomyces encodes a high number of natural product (NP) biosynthetic gene clusters (BGCs). Most of these BGCs are not expressed or are poorly expressed (commonly called silent BGCs) under traditional laboratory experimental conditions. These NP BGCs represent an unexplored rich reservoir of natural compounds, which can be used to discover novel chemical compounds. To activate silent BGCs for NP discovery, two main strategies, including the induction of BGCs expression in native hosts and heterologous expression of BGCs in surrogate Streptomyces hosts, have been adopted, which normally requires genetic manipulation. So far, various genome editing technologies have been developed, which has markedly facilitated the activation of BGCs and NP overproduction in their native hosts, as well as in heterologousStreptomyces hosts. In this review, we summarize the challenges and recent advances in genome editing tools for Streptomyces genetic manipulation with a focus on editing tools ...
TO THE EDITOR: Streptomyces spp. are aerobic, gram-positive bacteria of the order Actinomycetales, known for their ability to produce antimicrobial molecules such as streptomycin. Streptomyces spp., usually saprophytic to humans, can cause local cutaneous fistulized nodules known as actinomycetoma or mycetoma. Severe invasive infections have seldom been reported, but most cases reported have occurred in immunocompromised patients (1-5). We report a case of invasive pulmonary infection caused by a Streptomyces sp. in a splenectomized patient with sarcoidosis ...
Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics.(Chem. Biol.) [2001] ...
The enzyme, characterized from the bacterium Streptomyces hygroscopicus subsp. limoneus, is involved in the biosynthesis of the antifungal agent validamycin A.
Bio - Nata is produced from Streptomyces lydicus, a naturally occurring bacterium that is commonly found in soil environment. Bio - Nata is produced f...
Fourteen Streptomyces strains were isolated from soil samples of five different areas of Cukurova University. Four strains (S35, S47, S48, S50) had antibacterial activity against test organisms which were Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa and this antibacterial activity was evaluated by three different media. The strains S47 and S48 showed the biggest zone diameter against test organisms at glycerol-yeast agar medium, on the other hand, the strains S35 and S50 indicated the biggest zone diameter at glucose-asparagine agar medium. As a result, the group and activity of bioactive compounds produced by Streptomyces can be influenced by their nutritional conditions and environmental factors. ...
1) Streptomyces coelicolor A3(2). NCBI Taxonomy Browser. 29 April 2007. NCBI. (2) Conn, Jean E. The Pigment Production of Actinomyces coelicolor and A. violaceus-ruber. Journal of Bacteriology. 1943. Volume 46. p. 133-149. Link to Article (3) From Mapping to Mining the Streptomyces Genome. John Innes Centre Website. 2001. Link to Article (4) Thompson, Charles J., Dorris Fink, and Liem D. Nguyen. Principles of Microbial Alchemy: Insights from the Streptomyces coelicolor Genome Sequence. Genome Biology 3.7. (2002) Link to Article on PubMed (5) Kutzner, Hans J and Selman A. Waksman. Streptomyces coelicolor Muller and Streptomyces violaceoruber Waksman and Curtis, Two Distinctly Different Organisms. Journal of Bacteriology 78.4 (1959) p. 528-538. Link to Article (6) Bentley, S.D., K. F. Chater, A.-M. Cerdeño-Tárraga, G. L. Challis , N. R. Thomson, K. D. James, D. E. Harris, M. A. Quail, H. Kieser, D. Harper, A. Bateman, S. Brown, G. Chandra, C. W. Chen, M. Collins, A. Cronin, A. Fraser, ...
Streptomyces scabies or Streptomyces scabiei is a streptomycete bacterium species found in soils around the world. Unlike most of the 500 or so Streptomyces species it is a plant pathogen causing corky lesions to form on tuber and root crops as well as decreasing the growth of seedlings. Along with other closely related species it causes the potato disease common scab, which is an economically important disease in many potato growing areas. It was first described in 1892, being classified as a fungus, before being renamed in 1914 and again in 1948. Several other species of Streptomyces cause similar diseases to S. scabies but other, more closely related species, do not. The genome of S. scabies has been sequenced and is the largest Streptomyces genome known so far. The genome contains a pathogenicity island containing the genes required for S. scabies to infect plants, and which can be transferred between different species. S. scabies can produce several related toxins which are the most ...
en] The simplest model for the interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61 and beta-lactam antibiotics involves the three following steps: (a) the formation of a reversible equimolar enzyme - antibiotic complex; (b) the irreversible transformation of this complex into a modified enzyme - antibiotic complex; and (c) the breakdown of this latter complex and the concomitant release of a regenerated enzyme and a modified antibiotic molecule. The dissociation constant for step 1 and the rate constants for steps 2 and 3 were measured with various beta-lactam antibiotics. With antibiotic such as benzylpenicillin, which behaves as a good substrate, steps 1 and 2 occur at enzymic velocities, whereas step 3 occurs at a very low velocity and hence is responsible for the low efficiency of the overall process ...
Bleomycin is a broad-spectrum glycopeptide antitumor antibiotic produced by Streptomyces verticillus. Clinically, the mixture of bleomycin A2 and bleomycin B2 is widely used in combination with other drugs for the treatment of various cancers. As a secondary metabolite, the biosynthesis of bleomycin is precisely controlled by the complex extra-/intracellular regulation mechanisms, it is imperative to investigate the global metabolic and regulatory system involved in bleomycin biosynthesis for increasing bleomycin production. N-acetylglucosamine (GlcNAc), the vital signaling molecule controlling the onset of development and antibiotic synthesis in Streptomyces, was found to increase the yields of bleomycins significantly in chemically defined medium. To mine the gene information relevant to GlcNAc metabolism, the DNA sequences of dasR-dasA-dasBCD-nagB and nagKA in S. verticillus were determined by chromosome walking. From the results of Real time fluorescence quantitative PCR (RT-qPCR) and
Streptomyces species are considered exceptionally well endowed for chemical warfare, presumably allowing them to eliminate bacterial and fungal competitors in soil ecosystems. This was first realized over fifty years ago, and since then the majority of known antibiotics have been isolated from Streptomyces. Although thousands of antibiotics have been described, these are thought to represent only a small fraction of the repertoire of bioactive compounds produced by Streptomyces [4,5]. In addition, empirical screening using various assays has revealed that Streptomyces culture supernatants contain other pharmaceutically active compounds, such as anti-viral and anti-cancer compounds, modulators of immune responses, and various enzyme inhibitors, as well as herbicides, insecticides, and anti-parasitic compounds [4,6,7]. Furthermore, chemical screening methods have uncovered the remarkable structural diversity of these compounds. Genome sequences of Streptomyces species are now providing an ...
Streptomyces globisporus 1912 lnd-cluster region which flanks structural lndZ5-lndZ6 genes contains four open reading frames lndW, lndW2, lndYR and lndY. The latter one encodes putative proteinase which can regulate landomycin production and morphogenesis like LndYR. However, results of lndY overexpression and gene knockout showed that lndY did not participate in regulation of landomycin production and morphogenesis of Streptomyces globisporus 1912. Using transcriptional fusion of promoter lndWp to catechol dioxygenase reporter gene xylE the temporal character of interaction of promoter lndWp and repressor LndYR was studied.. ...
Detail záznamu - tmRNA of Streptomyces collinus and Streptomyces griseus during the growth and in the presence of antibiotics - Detail záznamu - Knihovna Akademie věd České republiky
Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology
To improve the fermentation production of transglutaminase (TGase) from Streptomyces mobaraensis for applications in the food industry, the atmospheric and room-temperature plasma (ARTP) mutagenesis was applied to breed S. mobaraensis mutants with increased TGase production. After eight rounds of iterative ARTP mutagenesis, four genetically stable mutants, Sm5-V1, Sm6-V13, Sm2-V10, and Sm7-V12, were identified, which showed increased TGase production by 27, 24, 24, and 19%, respectively. The best mutant Sm5-V1 exhibited a maximum TGase activity of 5.85 U/mL during flask fermentation. Compared to the wild-type strain, the transcription levels of the zymogen TGase genes in the mutants increased significantly as indicated by quantitative real-time PCR, while the gene nucleotide sequences of the mutants did not change at all. It was shown that the overexpression of TGase zymogen gene in the mutants contributes to the increase in TGase production. ARTP is a potentially efficient tool for microbial mutation
TY - JOUR. T1 - TrpM, a Small Protein Modulating Tryptophan Biosynthesis and Morpho-Physiological Differentiation in Streptomyces coelicolor A3(2). AU - Puglia, Anna Maria. AU - Botta, Luigi. AU - Giardina, Anna. AU - Gallo, Giuseppe. AU - Sutera, Alberto. AU - Palazzotto, Emilia. AU - Scaloni, Andrea. AU - Renzone, Giovanni. AU - Palazzotto, Emilia. PY - 2016. Y1 - 2016. N2 - In the model actinomycete Streptomyces coelicolor A3(2), small open reading frames encoding proteins with unknown functions were identified in several amino acid biosynthetic gene operons, such as SCO2038 (trpX) in the tryptophan trpCXBA locus. In this study, the role of the corresponding protein in tryptophan biosynthesis was investigated by combining phenotypic and molecular analyses. The 2038KO mutant strain was characterized by delayed growth, smaller aerial hyphae and reduced production of spores and actinorhodin antibiotic, with respect to the WT strain. The capability of this mutant to grow on minimal medium was ...
Self-resistance of the nourseothricin-producing strain Streptomyces noursei.: The nourseothricin producer Streptomyces noursei is resistant to its own antibioti
Malate synthases (MS) from Streptomyces coelicolor A3(2) and S. clavuligerus NRRL3585 were cloned by polymerase chain reaction into a glutathione S-transferase (GST) fusion expression vector and heterologously expressed in Escherichia coli. The fusion GST-MS construct improved the soluble expression of MS by approximately 10-fold compared to the soluble expression of nonfusion MS. With the significant improvement in levels of soluble MS, purification and subsequent cleavage of recombinant MS from GST were facilitated in this study. Using purified enzymes, optimized parameters, which achieved maximal specific activity, were established in the enzymatic assay for streptomycete MS. The average purified specific activities of S. coelicolorand S. clavuligerus MS were 26199 and 11821 nmol/mg min, respectively. Furthermore, enzymatic analysis revealed that the two streptomycete MS displayed a similar Km value for acetyl-CoA, but S. coelicolor MS had a Km value for glyoxylate that is approximately ...
Amicetin, an antibacterial and antiviral agent, belongs to a group of disaccharide nucleoside antibiotics featuring an alpha-(1 -> 4)-glycoside bond in the disaccharide moiety. In this study, the amicetin biosynthesis gene cluster was cloned from Streptomyces vinaceusdrappus NRRL 2363 and localized on a 37-kb contiguous DNA region. Heterologous expression of the amicetin biosynthesis gene cluster in Streptomyces lividans TK64 resulted in the production of amicetin and its analogues, thereby confirming the identity of the ami gene cluster. In silico sequence analysis revealed that 21 genes were putatively involved in amicetin biosynthesis, including 3 for regulation and transportation, 10 for disaccharide biosynthesis, and 8 for the formation of the amicetin skeleton by the linkage of cytosine, p-aminobenzoic acid (PABA), and the terminal (+)-alpha-methylserine moieties. The inactivation of the benzoate coenzyme A (benzoate-CoA) ligase gene amiL and the N-acetyltransferase gene amiF led to two ...
Organofluorine compounds are widely prepared throughout the chemicals industry, but their prepararion generally requires harsh fluorinating reagents and non-aqueous solvents. On the other hand, biology has hardly exploited organofluorine compounds. A very few organisms synthesize organofluorine metabolites, suggesting they have evolved a mechanism to overcome the kinetic desolvation barrier to utilizing F(-)(aq). Here, the purification and crystallization of an enzyme from Streptomyces cattleya which is responsible for the synthesis of the C-F bond during fluoroacetate and 4-fluorothreonine biosynthesis is reported. The protein crystallizes in space group C222(1), with unit-cell parameters a = 75.9, b = 130.3, c = 183.4 A, alpha = beta = gamma = 90 degrees. Data were recorded to 1.9 A at the ESRF. The structure of the protein should provide important insights into the biochemical process of C-F bond formation.. ...
The impact of different carbon sources on the antibiotic production by Streptomyces hygroscopicus CH-7 was studied with the main goal to increase the yield of antibiotics hexaene H-85 and elaiophylin. Glucose, as a basic carbon source in the nutrition medium, was replaced with glycerol, xylose, sorbose, melibiose, inulin, and mannitol (15 g/dm(3)). Insuring the maximum yields of hexaene and elaiophylin of 192 and 88 mu g/cm(3), respectively, glycerol was shown to be the best carbon source among the investigated ones ...
TY - JOUR. T1 - New non-quinone geldanamycin analogs from genetically engineered Streptomyces hygroscopicus. AU - Wu, Cheng Zhu. AU - Moon, An Na. AU - Jang, Jae Hyuk. AU - Lee, Dongho. AU - Kang, Sun Young. AU - Park, Joon Tae. AU - Ahn, Jong Seog. AU - Hwang, Bang Yeon. AU - Kim, Young Ho. AU - Lee, Hong Sub. AU - Hong, Young Soo. N1 - Funding Information: This work was supported in part by the 21C Frontier Microbial Genomics and Application Center and Global R&D Center program, the Ministry of Science and Technology, Republic of Korea and by a grant from KRIBB Research Initiative Program. We thank the Korea Basic Science Institute for the NMR measurements. PY - 2011/6. Y1 - 2011/6. KW - Hsp90 inhibitor. KW - biosynthetic engineering. KW - geldanamycin. KW - non-quinone geldanamycin. UR - http://www.scopus.com/inward/record.url?scp=79959736928&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=79959736928&partnerID=8YFLogxK. U2 - 10.1038/ja.2011.24. DO - ...
g- 1, 6 NAKAO ISHIDA ETAL 3,334,022 NEOCARZINOSTATIN PRODUCED BY STREPTOMYCES CARZINOSTATICUS VAR NEOCARZINOSTATICUS Filed April 26, 1965 2 Sheets-Sheet 1 NAKAO ISHIDA KEIZO MIYAZAKI KATSUO KUMAGAI MITSUO RIKIMARU MA SAl-HKO KUROYA INVENTORS. B. SIMONTON & HERBERT W TAYLOR,JR. ATTORNEYS. NAKAO-ISHIDA ETAL NEOCARZINOSTATIN PRODUCED BY STREPTOMYCES Aug. l 1967 3,334,022 NOSTATICUS VAR. NEOCARZINOSIATICUS v CARZI Filed April 26, 1965 2 Sheets-Sheet O O O O O O O om D P a f P Om \r fl cm 09 PERCENT TRANSMISSION IUA IAR KGAW AA M WK W Y IKRO OO O UUH AO A QTT AEA NKKMM N w w 2% K ON N R IOYO M T ABIT H WA W RWR TMEK R BR CRRH Y B United States Patent 3,334,022 NEOCARZINOSTATIN PRODUCED BY STREPTOMYCES CARZIZVOSTATIC US VAR. NEOCARZINOSTATICUS Nakao Ishida, 90 Kakogoro-cho; Keizo Miyazaki, 48 Kakyoin-dori; Katsuo Kumagai, 129 Tori-cho; and Mitsuo Rikimaru, 61 Kita-shichiban-cho, all of Sendai, Japan; and Masahiko Kuroya, Room 605, Empire Corp., 28 Daikyo-machi, Shinjuku-ku, Tokyo, Japan Filed Apr. ...
A homologue of the bacterial cell division gene ftsZ was cloned from the filamentous bacterium Streptomyces coelicolor. The gene was located on the physical map of the chromosome at about 11 oclock (in the vicinity of glkA, hisA and trpB). Surprisingly, a null mutant in which the 399-codon ftsZ open reading frame was largely deleted was viable, even though the mutant was blocked in septum formation. This indicates that cell division may not be essential for the growth and viability of S. coelicolor. The ftsZ mutant was able to produce aerial hyphae but was unable to produce spores, a finding consistent with the idea that ftsZ is required in order for aerial hyphae to undergo septation into the uninucleoid cells that differentiate into spores. ...
Cell division in the Gram-positive bacterium Streptomyces coelicolor starts with the assembly of the tubulin homologue FtsZ into a cytokinetic ring (the Z ring) at the site of septation. In stark contrast to the binary fission of most bacteria, the syncytial hyphal cells of S. coelicolor exploit two types of cell division with strikingly different outcomes depending on the developmental stage. The main goal of this study has been to identify developmental mechanisms that modulate this differential performance of the basic cell division machinery.. By isolation and characterization of a non-sporulating ftsZ mutant, we demonstrated that the requirements for Z-ring formation differ between the two types of septation. The ftsZ17(Spo) mutation abolished septation without overtly affecting vegetative growth. This mutant was defective in the assembly of FtsZ into regularly spaced Z rings in sporogenic hyphae, suggesting that the assembly of Z rings is developmentally controlled during ...
TY - JOUR. T1 - The ADEP biosynthetic gene cluster in Streptomyces hawaiiensis NRRL 15010 reveals an accessory clpP gene as a novel antibiotic resistance factor. AU - Thomy, Dhana. AU - Culp, Elizabeth. AU - Adamek, Martina. AU - Cheng, Eric Y.. AU - Ziemert, Nadine. AU - Wright, Gerard D.. AU - Sass, Peter. AU - Brötz-Oesterhelt, Heike. PY - 2019. Y1 - 2019. N2 - The increasing threat posed by multiresistant bacterial pathogens necessitates the discovery of novel antibacterials with unprecedented modes of action. ADEP1, a natural compound produced by Streptomyces hawaiiensis NRRL 15010, is the prototype for a new class of acyldepsipeptide (ADEP) antibiotics. ADEP antibiotics deregulate the proteolytic core ClpP of the bacterial caseinolytic protease, thereby exhibiting potent antibacterial activity against Gram-positive bacteria, including multiresistant pathogens. ADEP1 and derivatives, here collectively called ADEP, have been previously investigated for their antibiotic potency against ...
The antifungal polyene macrolide nystatin is produced by Streptomyces noursei ATCC 11455. The nystatin biosynthesis gene cluster of Streptomyces noursei has been cloned and sequenced, and a biosynthesis route has been predicted. In the present work, investigation of genes presumably involved in post-PKS modifications of nystatin is described. The aim of this work was to better understand the nystatin biosynthesis and to further use this information for generation of novel nystatin analogues. Two PKS-modifications of the nystatin molecule were targeted in this study: glycosylation with mycosamine at C-19 and oxidation of the exocyclic methyl group at C-16.. Two genes putatively involved in mycosamine biosynthesis (NysDIII and NysDII) and one in attachment of mycosamine to the nystatin aglycone (nysDI) have been identified in the nystatin gene cluster. Their functions have been suggested, respectively, as a putative mannose dehydratase, aminotransferase and a glycosyltransferase. The deoxysugar ...
Streptomyces sp. belongs to Streptomyces, the largest genus of Actinobacteria and the type genus of the family Streptomycetaceae.
In Streptomyces coelicolor, bldA encodes the only tRNA for a rare leucine codon, UUA. This tRNA is unnecessary for growth, but is required for some aspects of secondary metabolism and morphological development. We describe a transcriptomic and proteomic analysis of the effects of deleting bldA on cellular processes during submerged culture: conditions relevant to the industrial production of antibiotics. At the end of rapid growth, a co-ordinated transient up-regulation of about 100 genes, including many for ribosomal proteins, was seen in the parent strain but not the ΔbldA mutant. Increased basal levels of the signal molecule ppGpp in the mutant strain may be responsible for this difference. Transcripts or proteins from a further 147 genes classified as bldA-influenced were mostly expressed late in culture in the wild-type, though others were significantly transcribed during exponential growth. Some were involved in the biosynthesis of seven secondary metabolites; and some have probable roles in
Streptomyces are a genus of filamentous bacteria that includes over 500 species characterized by a fungal like mycelium body and the production of spores for reproduction. Found predominantly in soil and decaying vegetation, they are a major source of antibiotics (which is why Ginkgo studies them) and produce geosmin, a metabolite that gives soil its characteristic earthy rain smell; many species in the genus also produce vivid pigments as metabolites. Natsai Audrey Chieza, Ginkgos first creative resident used Streptomyces coelicolor to naturally dye silk, by culturing the bacteria directly onto the textile in different ways to produce different patterns. Ginkgo provided me with different Streptomyces cultures to test on my bioplastics and see if they could be cultured to dye the materials, transforming them as they also decomposed them. Kyle taught me how to successfully culture S. coelicolor in sterile conditions onto plain cellulose (rice paper) and thin wood substrates however it was ...
Streptomyces lividans CelB is a family-12 endoglucanase that hydrolyses cellulose with retention of anomeric configuration. A recent X-ray structure of the catalytic domain at 1.75 Å resolution has led to the preliminary assignment of Glu-120 and Glu-203 as the catalytic nucleophile and general acid-base respectively [Sulzenbacher, Shareck, Morosoli, Dupont and Davies (1997) Biochemistry 36, 16032-16039]. The present study confirms the identity of the nucleophile by trapping the glycosyl-enzyme intermediate with the mechanism-based inactivator 2´,4´-dinitrophenyl 2-deoxy-2-fluoro-β-d-cellobioside (2FDNPC). The kinetics of inactivation proceeded in a saturable fashion, yielding the parameters kinact = 0.29±0.02 min-1 and Kinact = 0.72±0.08 mM. Uncompetitive inhibition was observed at high concentrations of 2FDNPC (Ki = 9±1 mM), a behaviour that was also observed with the substrate 2´,4´-dinitrophenyl β-d-cellobioside (kcat = 40±1 s-1, Km = 0.35±0.03 mM, Ki = 24±4 mM). Protection ...
Classification of Raman spectra recorded from single cells is commonly applied to bacteria that exhibit small sizes of approximately 1 to 2 μm. Here, we study the possibility to adopt this classification approach to filamentous bacteria of the genus ,i,Streptomyces,/i,. The hyphae can reach extensive lengths of up to 35 μm, which can correspond to a single cell identified in light microscopy. The classification of Raman bulk spectra will be demonstrated. Here, ultraviolet resonance Raman (UV RR) spectroscopy is chosen to classify six ,i,Streptomyces,/i, species by the application of a tree-like classifier. For each knot of the hierarchical classifier, estimated classification accuracies of over 94% are accomplished. In contrast to the classification of bulk spectra, the classification of single-cell spectra requires a homogenous substance distribution within the cell. Consequently, the bacterial cell chemistry can be represented by one individual spectrum. This requirement is not fulfilled ...
Bringmann, Gerhard and Lang, Gerhard and Maksimenka, Katja and Hamm, Andreas and Gulder, Tobias A. M. and Dieter, Anke and Bull, Alan T. and Stach, James E.M. and Kocher, Niko and Muller, Werner E. G. and Fiedler, Hans-Peter (2005) Gephyromycin, the first bridged angucyclinone, from Streptomyces griseus strain NTK 14. Phytochemistry, 66 (11). pp. 1366-1373. ISSN 0031-9422 . (doi:https://doi.org/10.1016/j.phytochem.2005.04.010 ) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) ...
Streptomyces coelicolor colonies from higher education and researchin the Microbes section. Germs and bacteria a catalog of all known germs
Streptomyces spp. have developed complicated mechanisms to adapt their metabolism to the change of the extracellular environments (16). Among these mechanisms, cross talk between different regulators may integrate different signal inputs through fine-tuning the expression of key target genes (10, 15). A regulatory link between nitrogen metabolism and phosphate metabolism was proposed in S. coelicolor, which was coordinated by PhoP through directly binding to the promoters of the nitrogen metabolism-associated genes, including glnA, glnII, and amtB (14). However, the roadblock mechanism suggested for amtB was different from that of the proposed competitive binding for glnA and glnII.. Based on the DNase I footprinting data, we defined, in the present study, a new GlnR binding box comprising of an a3-b3 site in addition to the previously well characterized a1-b1 and a2-b2 sites in the promoter region of amtB (22). All of the three GlnR binding boxes were proven essential for GlnR-mediated ...
1JFR: Structure of a microbial homologue of mammalian platelet-activating factor acetylhydrolases: Streptomyces exfoliatus lipase at 1.9 A resolution.
Streptomyces sp. ZZ035 isolated from a folk medicinal soil sample in China showed remarkable antimicrobial activities. During the isolation of secondary metabolites, a white crystal powder (1) was isolated from the broth of this strain. Its nuclear magnetic resonance (NMR) and infrared (IR) spectra indicated that it was a complex composed of triglycerides. Next, six C15-17 long- chain fatty acids derived from these triglycerides were respectively identified as n-pentadecanoyl, 12-methyltetradecanoyl, 14-methyl pentadecanoyl, palmitoyl, 15-methyl hexadecanoyl and 14-methyl hexadecanoyl using the gas chromatography-mass spectroscopy (GC-MS) technology. Finally, the 13C and 1H assignments of 1 were achieved through the analyses of NMR data. Based on above, their detailed NMR spectroscopic elucidation and meticulous 13C, 1H assignments, especially the split peaks and coupling correlation of protons attached on the glycerol carbons, were performed for distinguishing triglycerides from other glycerides and
Seven new azalomycin F analogs (1-7) were isolated from the broth of mangrove Streptomyces sp. 211726, and respectively identified as 25-malonyl demalonylazalomycin F5a monoester (1), 23-valine demalonylazalomycin F5a ester (2), 23-(6-methyl)heptanoic acid demalonylazalomycins F3a ester (3), F4a ester (4) and F5a ester (5), 23-(9-methyl)decanoic acid demalonylazalomycin F4a ester (6) and 23-(10-methyl)undecanoic acid demalony lazalomycin F4a ester (7). Their structures were established by their spectroscopic data and by comparing with those of azalomycins F3a, F4a and F5a. Biological assays exhibited that 1-7 showed broad-spectrum antimicrobial and anti HCT-116 activities.
Sigma-Aldrich offers Sigma-A2411, Amphotericin B from Streptomyces sp. for your research needs. Find product specific information including CAS, MSDS, protocols and references.
ID S5VSK1_STRC3 Unreviewed; 468 AA. AC S5VSK1; DT 16-OCT-2013, integrated into UniProtKB/TrEMBL. DT 16-OCT-2013, sequence version 1. DT 25-OCT-2017, entry version 33. DE RecName: Full=Glutamate decarboxylase {ECO:0000256,RuleBase:RU361171}; DE EC=4.1.1.15 {ECO:0000256,RuleBase:RU361171}; GN ORFNames=B446_20640 {ECO:0000313,EMBL:AGS70940.1}; OS Streptomyces collinus (strain DSM 40733 / Tu 365). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=1214242 {ECO:0000313,EMBL:AGS70940.1, ECO:0000313,Proteomes:UP000015423}; RN [1] {ECO:0000313,Proteomes:UP000015423} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 40733 / Tu 365 {ECO:0000313,Proteomes:UP000015423}; RA Ruckert C., Szczepanowski R., Goesmann A., Pross E.K., Musiol E.M., RA Blin K., Wohlleben W., Puhler A., Weber T., Kalinowski J.; RT The complete genome sequence of Streptomyces collinus Tu 365.; RL Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ...
Breast cancer is the most common form of cancer in Canadian women. Despite numerous therapeutic options, multidrug resistance (MDR) remains an obstacle to successful therapy.. Jadomycins are natural products derived from the soil bacteria Streptomyces venezuelae ISP5230 and maintain cytotoxicity in multidrug resistant human breast cancer cell lines. The objectives were to determine the single dose pharmacokinetics and safety of jadomycins and their effects on 4T1 breast primary tumors and lung metastasis in mice.. In the pharmacokinetic studies, intraperitoneal-administered jadomycins B, S, and F were rapidly absorbed, achieved serum concentration in the predicted therapeutic range and had a biphasic distribution and elimination profile. In tumor studies jadomycin B partially reduced tumor volumes and lung metastasis. In conclusion, jadomycins are safe to administer chronically to mice at doses up to 13.8 mg/kg twice daily. The partial anti-tumor and anti-metastatic effects of jadomycin B were ...
Novel compounds are produced by the fermentation of a nutrient medium with the previously undescribed microorganism Streptomyces avermitilis. They may be isolated by solvent extraction and chromatographic fractionation techniques. The compounds, which are described generically as C-076 have significant parasiticidal activity. The compounds may be included in compositions for the oral or parenteral administration to animals for the prevention and cure of parasitic infections.
The publication of the S. coelicolor genome sequence has stimulated interest in the possibility of rationally engineering strains by predicting the effect(s) of altering specific genes on secondary metabolite production. The work described here aims to design a general approach that could be applied to enhance the production of commercially important antibiotics that have similar biosynthetic routes as actinorhodin (ACT). It has been observed that a negative correlation between ACT production and the carbon flux through the pentose phosphate pathway (PPP) exists. As a result, strains with deletions for either one of the two zwf isogenes (encoding the enzyme that catalyses the initial PPP reaction) and for the devB gene (whose product catalyses the subsequent reaction in the pathway) were constructed and assessed. All strains, with the exception of the devB mutant, were found to generate increased levels of ACT compared to the parental strain. In addition to these strains, the above genotypes ...
Matt Hutchings Key Research Interests We are interested in how bacteria interact with the environment and how they sense and respond to environmental signals. All bacteria contain a cell envelope which consists of at least one cell membrane and (in most cases) a cell wall. Bacteria interact with their environment using cell surface proteins which are anchored either in the cell wall or the cell membrane. We are characterising two classes of these proteins in the filamentous bacteria Streptomyces. The first class are called sensor kinases and they span the membrane and transmit signals from the outside to the inside of the cell. They pass these signals to cognate response regulator proteins inside the cell and these bring about a response to the original signal, usually by altering gene expression. The second class are called lipoproteins and are attached by a lipid modification to the outside of the cell membrane. They have diverse roles including the scavenging of nutrients, surface attachment, ...