en] The activity of the D-alanyl-D carboxypeptidase from the penicillin-resistant Streptomyces albus G is not or very little affected by penicillins and related antibiotics. The molecular basis for the mechanism of action of penicillin is discussed. The Streptomyces albus G D-alanyl-D carboxypeptidase appears as a model for the study of a mechanism of penicillin resistance that does not involve the enzymatic degradation of the antibiotic. Vancomycin and ristocetin are shown to inhibit the hydrolysis of sensitive peptides by the Streptomyces albus G D-alanyl-D carboxypeptidase and the mechanism of inhibition is discussed ...
Streptomyces thermoviolaceus ChiS protein: similar to histidine kinases; a member of a two-component sensor-regulator system; isolated from Streptomyces thermoviolaceus; amino acid sequence in first source; GenBank AB016841
Streptomyces isolates have yielded the majority of human, animal, and agricultural antibiotics, as well as a number of fundamental chemotherapy medicines. Streptomyces is the largest antibiotic-producing genus of actinobacteria, producing chemotherapy, antibacterial, antifungal, antiparasitic drugs, and immunosuppressants. Streptomyces isolates are typically initiated with the aerial hyphal formation from the mycelium. Streptomyces, yielded the medicines doxorubicin (Doxil), daunorubicin (DaunoXome), and streptozotocin (Zanosar). Doxorubicin is the precursor to valrubicin (Valstar), myocet, and pirarubicin. Daunorubicin is the precursor to idarubicin (Idamycin), epirubicin (Ellence), and zorubicin. Streptomyces is the original source of dactinomycin (Cosmegen), bleomycin (Blenoxane), pingyangmycin (Bleomycin A5), mitomycin C (Mutamycin), rebeccamycin, staurosporine (precursor to stauprimide and midostaurin), neothramycin, aclarubicin, tomaymycin, sibiromycin, and mazethramycin. Derivatives of ...
P,Following translocation, bacterial lipoproteins are lipidated by lipoprotein diacylglycerol transferase (Lgt) and cleaved of their signal peptides by lipoprotein signal peptidase (Lsp). In Gram-negative bacteria and mycobacteria, lipoproteins are further lipidated by lipoprotein N-acyl transferase (Lnt), to give triacylated lipoproteins. Streptomyces are unusual amongst Gram-positive bacteria because they export large numbers of lipoproteins via the twin arginine protein transport (Tat) pathway. Furthermore, some Streptomyces species encode two Lgt homologues and all Streptomyces species encode two homologues of Lnt. Here we characterize lipoprotein biogenesis in the plant pathogen Streptomyces scabies and report that lgt and lsp mutants are defective in growth and development while only moderately affected in virulence. Lipoproteins are lost from the membrane in an S. scabies lgt mutant but restored by expression of Streptomyces coelicolor lgt1 or lgt2 confirming that both encode functional ...
Bagramyan, K., et al. Mass Spectrometric Resurrection of SHA, an LRhamnose and Beta-D-Galactose Binding Lectin from the Lost Strain Streptomyces 27S5. ASMS. 0, ThP 307. 01/06/2015.. ...
Mangrove Streptomyces represent a rich source of novel bioactive compounds in medicinal research. A novel alkaloid, named 1-N-methyl-3-methylamino-[N-butanoic acid-3¢-(9¢-methyl-8¢-propen-7¢-one)-amide]-benzo[f][1,7]naphthyridine-2-one (1) was isolated from Streptomyces albogriseolus originating from mangrove sediments. The structure of compound 1 was elucidated by extensive spectroscopic data analyses and verified by the 13C-NMR calculation at the B3LYP/6-311+G(2d,p) level of theory.
ID H2K393_STRHJ Unreviewed; 512 AA. AC H2K393; DT 21-MAR-2012, integrated into UniProtKB/TrEMBL. DT 21-MAR-2012, sequence version 1. DT 22-NOV-2017, entry version 39. DE RecName: Full=Probable DNA ligase {ECO:0000256,HAMAP-Rule:MF_00407}; DE EC=6.5.1.1 {ECO:0000256,HAMAP-Rule:MF_00407}; DE AltName: Full=Polydeoxyribonucleotide synthase [ATP] {ECO:0000256,HAMAP-Rule:MF_00407}; GN Name=lig {ECO:0000256,HAMAP-Rule:MF_00407}; GN OrderedLocusNames=SHJG_2641 {ECO:0000313,EMBL:AEY87915.1}; OS Streptomyces hygroscopicus subsp. jinggangensis (strain 5008). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=1133850 {ECO:0000313,EMBL:AEY87915.1, ECO:0000313,Proteomes:UP000007170}; RN [1] {ECO:0000313,Proteomes:UP000007170} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=5008 {ECO:0000313,Proteomes:UP000007170}; RA Wu H., Bai L.; RT "Genomic analysis of Streptomyces hygroscopicus subsp. jinggangensis RT 5008."; RL Submitted (JAN-2012) to the ...
The reactions of beta-lactamases of Actinomadura R39 and Streptomyces albus G with clavulanate proceed along branched pathways. Both enzymes perform the hydrolysis of this beta-lactam with rather high efficiencies (kcat. = 18s-1 and 52s-1 respectively). If large clavulanate/enzyme ratios are used, complete inactivation of the enzymes is observed. At lower ratios, inactivation is only partial. Irreversible inactivation occurs after 400 and 20000 turnovers for the A. R39 and S. albus G enzymes respectively. With the A. R39 beta-lactamase, a transiently inhibited complex is also formed that remains undetectable with the S. albus G beta-lactamase. Kinetic models are presented and studied for the interaction between clavulanate and both enzymes. A tentative general reaction scheme is also discussed.. ...
Streptomyces violaceoruber ATCC ® BAA-471™ Designation: John Innes Centre M145 TypeStrain=False Application: The parental strain, Streptomyces coelicolor A3(2), was used worldwide in genetic studies.
Coloured scanning electron micrograph (SEM) of Streptomyces hygroscopicus, Gram-positive, aerobic, filamentous, biofilm-forming, rod prokaryote (bacterium). Streptomyces sp. belong to the Actinomycetes group and are bacteria that share many characteristics with the fungi. They grow usually as filaments (chains of cells) and often branch to form a network of filaments (mycelium) in the soil. These soil bacteria are responsible for the musty odour of soil. This strain of Streptomyces hygroscopicus produces the antibiotic, milbemycin, which is used as an insecticide and also to control certain parasitic infections in animals. It is also a biofilm forming bacterium. Biofilms are primarily accumulations of bacteria in aqueous environments. They form when bacteria secrete slimy, mucilaginous materials that provide the microorganisms with a means of attachment to moist surfaces. Magnification: x1,600 when shortest axis printed at 25 - Stock Image C032/2295
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Strains VIM M 10366(T), YIM M 10378(T) and YIM M 10400(T) were isolated from marine sediments collected from the Xisha Islands in the South China Sea. All three isolates were able to grow optimally at pH 7.0, 28-37 degrees C and 0-3% (w/v) NaCl. Comparison of 16S rRNA gene sequences showed that these strains are members of the genus Streptomyces, exhibiting moderately high 16S rRNA gene sequence similarities of 97.0-98.8% to members of the most closely related Streptomyces species. Morphological characteristics, physiological characteristics and compositions of whole-cell sugars and phospholipids are consistent with the diagnostic characteristics of the genus Streptomyces, but still allowed differentiation amongst the three strains and their neighbours. Based on 16S rRNA gene sequence analysis, DNA DNA relatedness, phenotypic characteristics and chemotaxonomic data, strains VIM M 10366(T), VIM M 10378(T) and VIM M 10400(T) were identified as members of three novel species of the genus ...
Read "Properties of lanK-based regulatory circuit involved in landomycin biosynthesis in Streptomyces cyanogenus S136, Russian Journal of Genetics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Transfer of Streptosporangium indianense Gupta 1965 to the genus Streptomyces Waksman and Henrici as Streptomyces indiaensis (Gupta 1965) comb. nov. is proposed on the basis of morphological and chemical properties. Spore chains such as those of typical streptomycetes were formed in the early stage of culture of S. indianense. True sporangia were not observed. Whole-cell hydrolysates contained ll-diaminopimelic acid. Compositions of cellular fatty acids and isoprenoid quinones were similar to those of streptomycetes. The type strain of Streptomyces indiaensis is JCM 3053T (= KCC A-0053, ATCC 33330, CBS 560.75, IFO 13964, and NCIB 9794).
Taxonomy of the species Streptomyces bambergiensis Wallhäusser et al. 1966 (Approved Lists 1980) pro synon. Streptomyces prasinus Ettlinger et al. 1958 (Approved Lists 1980) emend. Labeda et al. 2016
The cancer-cell-cytotoxicity-guided fractionation of the acetone extracts of two cultured marine-derived Streptomyces strains belonging to the MAR4 group yielded six new napyradiomycins, compounds A-F (1-6), together with three known compounds, napyradiomycins B2-B4 (7-9). Napyradiomycins 1-4 are new members of the napyradiomycin C-type meroterpenoids, which possess a linear monoterpene bridge between C-7 and C-10a. Compound 4 has an additional tetrahydropyran ring fused to the phenol moiety. Compounds 5-9 are related to the napyradiomycin B-type meroterpenoids. The structures of all new compounds were assigned by interpretation of 1D and 2D NMR, MS, and other spectroscopic data. The relative configurations were assigned based upon interpretation of ROESY 2D NMR experiments. The cytotoxicity of 1-9 against the human colon carcinoma cell line HCT-116 and their antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA) are presented.. ...
Streptomyces violaceoruber ATCC ® BAA-471D-5™ Designation: Genomic DNA from M145 (ATCC BAA-471) TypeStrain=False Application:
Spectrophotometric determination of the ionophore compounds and mass spectrometry of the biosynthesis products revealed that the cells of Streptomyces chrysomallus subsp. macrotetrolidi during the lag phase permanently contained practically constant though insignificant (about 20 to 40 nmol/mg of protein) amounts of biosynthetic precursors of macrotetrolides, oligomers of nactinic acids. The oligomers of nactinic acids had antibiotic activity and in an amount of 2.5 micrograms/ml inhibited the growth of Bacillus mycoides. The oligomers of nactinic acids had ionophore properties and were highly labile with respect to inorganic cations. The presence of sodium in the extragent stabilized the calcium monomer, a trimer of nonactinic acid, and promoted washing off the substances of the nactinic nature from the cells. The cations of ammonia and possibly potassium stabilized the dimer and tetramer of nonactinic acid forming a more hydrophobic complex by comparison with the initial compounds.
Streptomyces peucetius DnrS protein: involved in daunorubicin biosynthesis in Streptomyces peucetius; encodes a glycosyltransferase; amino acid sequence given in first source; GenBank L47164
Original publication: Krainsky A. Die Aktinomyceten und ihre Bedeutung in der Natur. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abteilung II 1914; 41:649-688. Nomenclatural status: not validly published Correct name: Streptomyces diastatochromogenes (Krainsky 1914) Waksman and Henrici 1948 (Approved Lists 1980) ...
FR-900520 and FR-900523, novel neutral macrolide immunosuppressants, were isolated from the cultured broth of Streptomyces hygroscopicus subsp. yakushimaensis No. 7238. Their molecular formulae were determined as C43H69NO12 and C42H67NO12, respectively. The compounds suppressed immune response in vitro. IC50 values of FR-900520 and FR-900523 for mouse mixed lymphocyte reaction were 0.55 nM and 1.6 nM, respectively. FR-900520, the major component, clearly prolonged skin allograft survival in rats.
TY - JOUR. T1 - 12T061A and 12T061C, two new julichrome family compounds, as radical scavengers from Streptomyces sp. AU - Komoda, Toshikazu. AU - Saeki, Naoko. AU - Koseki, Yoshitaka. AU - Kiyota, Hiromasa. PY - 2016/2/29. Y1 - 2016/2/29. N2 - We identified two new radical scavengers, 12T061A (1, C19H20O7) and 12T061C (2, C20H22O7), from a culture of the Streptomyces sp. Spectroscopic analysis indicated that these compounds are new julichrome family compounds. Compounds 1 and 2 showed radical-scavenging activity with an ED50 of 370 μM and 18 μM, respectively. Moreover, 1 showed tumor cell growth suppressive activity in HepG2 cells, (IC50: 3.6 μM); however, no suppressive activity was shown in 2 (IC50: , 100 μM).. AB - We identified two new radical scavengers, 12T061A (1, C19H20O7) and 12T061C (2, C20H22O7), from a culture of the Streptomyces sp. Spectroscopic analysis indicated that these compounds are new julichrome family compounds. Compounds 1 and 2 showed radical-scavenging activity ...
11181344] An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57. (Antimicrob Agents Chemother. , 2001 ...
Macro-photograph of a colony of the soil bacterium Streptomyces lividans. Streptomyces species are natural producers of a number of antibiotics, including streptomycin. A member of the Actinomycetes group, they share many characteristics with the fungi. The bacteria grow in the soil as a branching network of filaments, known as a mycelium. They also produce aerial mycelium, from the tips of which develop chains of spores. When the spores are mature, they rupture and are dispersed on the wind. - Stock Image B240/0019
High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherechia coli. Isolation and antifungal and antioomycete activities of aerugine produced by Pseudomonas fluorescens strain MM-B16
Sequence analysis of a 3.4-kb region Streptomyces peucetius daunorubicin (DNR) gene cluster established the presence of the dnrH and dnmT genes. In dnrH mutants, DNR production increased 8.5-fold, compared with that in the wild-type strain, while dnmT mutants accumulated epsilon-rhodomycinone (RHO), which normally becomes glycosylated in daunorubicin biosynthesis. Hence, dnmT may be involved in the biosynthesis or attachment of daunosamine to RHO or in the regulation of this process. Since the DnrH protein is similar to known glycosyl transferases, this protein may catalyze the conversion of DNR to its polyglycosylated forms, known as baumycins. Overexpression of dnmT in the wild-type and dnrH mutant strains resulted in a major decrease in RHO accumulation and increase in DNR production. ...
TO THE EDITOR: Streptomyces spp. are aerobic, gram-positive bacteria of the order Actinomycetales, known for their ability to produce antimicrobial molecules such as streptomycin. Streptomyces spp., usually saprophytic to humans, can cause local cutaneous fistulized nodules known as actinomycetoma or mycetoma. Severe invasive infections have seldom been reported, but most cases reported have occurred in immunocompromised patients (1-5). We report a case of invasive pulmonary infection caused by a Streptomyces sp. in a splenectomized patient with sarcoidosis ...
Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics.(Chem. Biol.) [2001] ...
The enzyme, characterized from the bacterium Streptomyces hygroscopicus subsp. limoneus, is involved in the biosynthesis of the antifungal agent validamycin A.
Bio - Nata is produced from Streptomyces lydicus, a naturally occurring bacterium that is commonly found in soil environment. Bio - Nata is produced f...
Streptomyces thermocarboxydus is a streptomycete bacterium species. It is moderately thermophilic and carboxydotrophic, with type strain AT37. Kim, S. B.; Falconer, C.; Williams, E.; Goodfellow, M. (1998). "Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil". International Journal of Systematic Bacteriology. 48 (1): 59-68. doi:10.1099/00207713-48-1-59. ISSN 0020-7713. PMID 9542077. Stach JE, Maldonado LA, Masson DG, Ward AC, Goodfellow M, Bull AT (October 2003). "Statistical approaches for estimating actinobacterial diversity in marine sediments". Applied and Environmental Microbiology. 69 (10): 6189-200. doi:10.1128/aem.69.10.6189-6200.2003. PMC 201225 . PMID 14532080. Retrieved 2013-09-08. Baur, Stefanie; Niehaus, Jörg; Karagouni, Amalia D; Katsifas, Efstathios A; Chalkou, Kalliopi; Meintanis, Christos; Jones, Amanda L; Goodfellow, Michael; Ward, Alan C; Beil, Winfried; Schneider, Kathrin; ...
A novel ikarugamycin-producing actinomycete, designated strain NEAU-Da3T, was isolated from soybean root [Glycine max (L.) Merr.] and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-Da3T belonged to the genus Streptomyces , and was most closely related to Streptomyces carpaticus NRRL B-16359T (99.5 %), Streptomyces cheonanensis VC-A46T (99.3 %) and Streptomyces xiamenensis MCCC 1A01550T (97.2 %); similarities to other type strains of species of the genus Streptomyces were lower than 97.1 %. The maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with S. carpaticus NRRL B-16359T, S. cheonanensis VC-A46T and S. xiamenensis MCCC 1A01550T. This branching pattern was also supported by the tree reconstructed with the neighbour-joining method. A comparative study between strain NEAU-Da3T and the type strains of the closest related species of the genus Streptomyces
In this study, the evolution of cellulose degradation was a defining event in the history of life. Without efficient decomposition and recycling, dead plant biomass would quickly accumulate and become inaccessible to terrestrial food webs and the global carbon cycle. On land, the primary drivers of plant biomass deconstruction are fungi and bacteria in the soil or associated with herbivorous eukaryotes. While the ecological importance of plant-decomposing microbes is well established, little is known about the distribution or evolution of cellulolytic activity in any bacterial genus. Here we show that in Streptomyces, a genus of Actinobacteria abundant in soil and symbiotic niches, the ability to rapidly degrade cellulose is largely restricted to two clades of host-associated strains and is not a conserved characteristic of the Streptomyces genus or host-associated strains. Our comparative genomics identify that while plant biomass degrading genes (CAZy) are widespread in Streptomyces, key ...
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Streptomycetes differ from other prokaryotic organisms in their mycelial life cycle and in possessing a large, linear, GC-rich chromosome. To deduce structural features of the Streptomyces origin of chromosomal replication, the oriC sequences of three Streptomyces species (S. antibioticus, S. chryso …
Die Vermehrung von Streptomyces albus unter Nutzung von Stoffwechselendprodukten und Leibessubstanzen einer absterbenden Para-B-Kultur führt zu Rückwirkungen auf die Salmonellen. Bezogen auf den...
1) De Meester F, et al. (1987) The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.. Biochem J 244(2):427-32 PubMed: 2822004 ...
Modification of the Streptomyces R61 DD-peptidase by N-bromosuccinimide resulted in a rapid loss of enzyme activity. In consequence, the role of the enzymes two tryptophan residues was investigated by site-directed mutagenesis. Trp271 was replaced by Leu. The modification yielded a stable enzyme whose structural and catalytic properties were similar to those of the wild-type protein. Thus the Trp271 residue, though almost invariant among the beta-lactamases of classes A and C and the low-Mr penicillin-binding proteins, did not appear to be essential for enzyme activity. Mutations of the Trp233 into Leu and Ser strongly decreased the enzymic activity, the affinity for beta-lactams and the protein stability. Surprisingly, the benzylpenicilloyl-(W233L)enzyme deacylated at least 300-fold more quickly than the corresponding acyl-enzyme formed with the wild-type protein and gave rise to benzylpenicilloate instead of phenylacetylglycine. This mutant DD-peptidase thus behaved as a weak ...
Streptococcus bacteria. Coloured scanning electron micrograph (SEM) of chains of Streptococcus bacteria with Streptomyces coelicoflavus bacteria in the background. Streptococcus bacteria are an example of cocci (round bacteria) that form chains. There are over 50 species of Streptococcus bacteria, some of which cause disease in humans. Streptomyces coelicoflavus is a filamentous soil bacterium. - Masterfile - Premium Royalty-Free, Code: 679-08425083
Ionomycin, Calcium Salt, Streptomyces conglobatus - CAS 56092-82-1 - Calbiochem Ionomycin, Calcium Salt, Streptomyces conglobatus, CAS 56092-82-1, is useful in cell activation experiments when calcium dose-response data are not required. - Find MSDS or SDS, a COA, data sheets and more information.
1JIE: The 1.6-A crystal structure of the copper(II)-bound bleomycin complexed with the bleomycin-binding protein from bleomycin-producing Streptomyces verticillus.
IS493 is an insertion sequence isolated from Streptomyces lividans by a method designed to trap transposable elements. IS493 was converted to functional transposons by cloning antibiotic-resistance-encoding genes between ORF-A and ORF-B of IS493 or near the left-end inverted repeat of the element. …
Despite screening various growth media, we failed to detect the production of any 2-hydroxyphenylthiazoline-containing metabolites by S. venezuelae. This is potentially explained by the low levels of sven0516 expression in the bldM mutant (Fig. 3), which is surprising given that sven0517 is likely to be in the same operon, and possibly reflects differential mRNA stability for the two genes. We therefore elected to express the sven0503-sven0517 gene cluster in the engineered host S. coelicolor M1152.23 A clone (SV-2_E03) from an ordered genomic cosmid library of the S. venezuelae chromosome containing a segment extending from sven0496 to sven0518 was PCR-targeted in Escherichia coli with a 5.2 kb SspI fragment from pIJ10702 that contains oriT, and the øC31 integrase gene and phage attachment site (attP). The resulting cosmid, SV-2_E03::SspI, was introduced into S. coelicolor M1152 by conjugation, whereupon it integrated into the chromosomal øC31 attB site. Wild type S. coelicolor M1152 and the ...
Toxin-antitoxin (TA) systems are small genetic elements that are ubiquitous in prokaryotes. Most studies on TA systems have focused on commensal and pathogenic bacteria; yet very few studies have focused on TAs in marine bacteria, especially those isolated from a deep sea environment. Here, we characterized a type II VapC/VapB TA system from the deep-sea derived Streptomyces sp. SCSIO 02999. The VapC (virulence-associated protein) protein belongs to the PIN (PilT N-terminal) superfamily. Overproduction of VapC strongly inhibited cell growth and resulted in a bleb-containing morphology in E. coli. The toxicity of VapC was neutralized through direct protein-protein interaction by a small protein antitoxin VapB encoded by a neighboring gene. Antitoxin VapB alone or the VapB/VapC complex negatively regulated the vapBC promoter activity. We further revealed that three conserved Asp residues in the PIN domain were essential for the toxic effect of VapC. Additionally, the VapC/VapB TA system stabilized plasmid
MK800-62F1, a New Inhibitor of Apoptotic Cell Death, from Streptomyces diastatochromogenes MK800-62F1. I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Biological Activity.:I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Biological Activity (2000 ...
Harmonised classification and labelling is a legally binding classification and labelling for a substance, agreed at European Community level. Harmonisation is based on the substances physical, toxicological and eco-toxicological hazard assessment. The Hazard classification and labelling section uses the signal word, pictogram(s) and hazard statements of the substance under the harmonised classification and labelling (CLH) as its primary source of information.. If the substance is covered by more than one CLH entry (e.g. disodium tetraborate EC no. 215-540-4, is covered by three harmonisations: 005-011-00-4; 005-011-01-1 and 005-011-02-9), CLH information cannot be displayed in the InfoCard as the difference between the CLH classifications requires manual interpretation or verification. If a substance is classified under multiple CLH entries, a link to the C&L Inventory is provided to allow users to view CLH information associated with the substance, instead of having the information ...
Puromycin (PM) is classified into a family of nucleoside antibiotics together with blasticidin S (BS). PNI-producing Streptomyces alboniger is known to express a PM-inactivating enzyme as a self-resistance determinant, which catalyzes the acetylation of PM. We have shown that, although BS-producing Streptomyces morookaensis also produces a PM-inactivating enzyme, it catalyzes the hydrolysis of an amide linkage between the aminonucleoside and 0-methyl-L-tyrosine moiety of PM. In the present study, we cloned and characterized a gene encoding PM hydrolase (PMH) from BS-producing S. morookaensi JCM4673. The nucleotide sequence analysis suggests that an open reading frame consisting of 1986 bp is a gene for PMH and encodes a protein consisting of 662 amino acids with a calculated molecular mass of 71,260 Da. The molecular mass of the recombinant PMH, which was produced using an Escherichia coli host-vector system, was the same as that of PMH purified from the JCM4673 strain. Our biochemical study of ...
Septacin, a new antitumor and antifungal antibiotic produced by Streptomyces fimbriatus Antimicrob Agents Chemother 1963, 83-88, ...
Several versatile, multi-copy, promoter-probe plasmid vectors have been constructed that replicate in a wide range of Streptomyces species. Transcriptional activity is detected by the expression of a
TY - JOUR. T1 - A combined approach of classical mutagenesis and rational metabolic engineering improves rapamycin biosynthesis and provides insights into methylmalonyl-CoA precursor supply pathway in Streptomyces hygroscopicus ATCC 29253. AU - Jung, Won Seok. AU - Yoo, Young Ji. AU - Park, Je Won. AU - Park, Sung Ryeol. AU - Han, Ah Reum. AU - Ban, Yeon Hee. AU - Kim, Eun Ji. AU - Kim, Eunji. AU - Yoon, Yeo Joon. PY - 2011/9/1. Y1 - 2011/9/1. N2 - Rapamycin is a macrocyclic polyketide with immunosuppressive, antifungal, and anticancer activity produced by Streptomyces hygroscopicus ATCC 29253. Rapamycin production by a mutant strain (UV2-2) induced by ultraviolet mutagenesis was improved by approximately 3.2-fold (23.6 mg/l) compared to that of the wild-type strain. The comparative analyses of gene expression and intracellular acyl-CoA pools between wild-type and the UV2-2 strains revealed that the increased production of rapamycin in UV2-2 was due to the prolonged expression of rapamycin ...
Three cyclic lipopeptides, including one known (1) and two new (2 and 3) compounds, that possess the rare enamide linkage group were discovered from Streptomyces sp. KCB14A132, an actinobacterium isolated from a soil sample collected from Jeung Island, Korea. The NMR and MS-based characterization showed that they differed in the amino acid residues in the peptide backbone. Application of Marfeys ...
The Procarta researchers found that the bacterium Streptomyces produces a particularly high yield of enzymes and proteins eriacta vs viagra . Unusually, it can also secrete the proteins they produce, so that they do not have to be extracted. Streptomyces enzyme producing bacterium with bells and whistles, represent an important contribution to a market already predicted to make that worth 400 million euros by 2010, said Dr. McArthur.. At the same time, stressed, stressed, stress hormones through the placenta passed on to the fetus and may neurodevelopment stress response. Under such conditions, the part of the childs brain with with stress can be programmed incorrectly in utero - the brain does not develop as it would be under ideal circumstances, if this theory is correct, determine you would if stressful events occur to people. That were smaller babies would rather depressed or anxious, said Colman. Effects on thetable strengths of this study are the nationally representative sample, the ...
Structural elements of the Streptomyces oriC region and their interactions with the DnaA protein. Transcription analysis of the dnaA gene and oriC region of the chromosome of Mycobacterium smegmatis and Mycobacterium bovis BCG, and its regulation by the DnaA protein
1M03: Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.
Designation of Streptomycete 16S and 23S rRNA-based target regions for oligonucleotide probes.: The 16S and 23S rRNA of various Streptomyces species were partia
Streptomyces:Media_and_Solutions/Antibiotic_&_Resistance_Marker_Information , Antibiotic & Resistance Marker Information]] ,, There is a problem with the this link in the dewikified version, hence the & in the file name was changed to and ...
Pfizer - Principal Scientist Streptomyces Molecular Biology/Genetics - Kalamazoo - **ROLE SUMMARY** The position of Principle Scie - Black Enterprise
Common approaches to antibiotic discovery include small-molecule screens for growth inhibition in target pathogens and screens for inhibitors of purified…
A longstanding goal in the Keasling Lab centers on the characterization and development of microbial hosts beyond those traditionally associated with synthetic biology and metabolic engineering (E. coli and S. cerevisiae). This includes developing detailed multi-omic understanding of select microbes and improved biological tools to manipulate these hosts. Recently, we have become ever more interested studying the complex metabolism of Streptomyces species and developing tools to allow reliable and rapid metabolic engineering in this bacterial genus. Given the immense scope of secondary metabolites produced by these bacteria and other favorable properties unique to Streptomyces venezuelae, we are committed to developing this bacterium for routine biotechnology efforts. Upcoming reports will detail the generation of improved biological tools (i.e., fluorescent reporters, improved promoters, Cas9 genome editing systems, etc.) that facilitate efforts aimed toward producing non-native products. ...
1) Dehottay P, et al. (1987) Nucleotide sequence of the gene encoding the Streptomyces albus G beta-lactamase precursor.. Eur J Biochem 166(2):345-50 PubMed: 3038538 ...
in Protein Science : A Publication of the Protein Society (2000), 9(3), 466-75. In a general approach to the understanding of protein adaptation to high temperature, molecular models of the closely related mesophilic Streptomyces sp. S38 Xyl1 and thermophilic Thermomonospora fusca ... [more ▼]. In a general approach to the understanding of protein adaptation to high temperature, molecular models of the closely related mesophilic Streptomyces sp. S38 Xyl1 and thermophilic Thermomonospora fusca TfxA family 11 xylanases were built and compared with the three-dimensional (3D) structures of homologous enzymes. Some of the structural features identified as potential contributors to the higher thermostability of TfxA were introduced in Xyl1 by site-directed mutagenesis in an attempt to improve its thermostability and thermophilicity. A new Y11-Y16 aromatic interaction, similar to that present in TfxA and created in Xyl1 by the T11Y mutation, improved both the thermophilicity and thermostability. ...
Literature References: Antibiotic substance produced by a streptomycete from soil near Oswego, N.Y. Has a macrolide structure (12-membered lactone ring, compare Picromycin). Isoln and antibacterial activity: Donin et al., Antibiot. Annu. 1, 179 (1953-4). Production using Streptomyces venezuelae cultures: Dutcher et al., US 2916483 (1959 to Olin Mathieson). Structure: C. Djerassi, J. A. Zderic, J. Am. Chem. Soc. 78, 6390 (1956). Absolute configuration: Rickards, Smith, Tetrahedron Lett. 1970, 1025; Manwaring et al., ibid. 1029. Biosynthesis: Birch et al., J. Chem. Soc. 1964, 5274. Synthesis: Masamune et al., J. Am. Chem. Soc. 97, 3512 (1975). Possible mechanism of action: Wilhelm et al., Antimicrob. Agents Chemother. 1967, 236. ...
190 Frelinghuysen Road, Busch Campus Piscataway, NJ 08854-8020 Telephone: 732-445-3425; Fax: 732-445-5735 Joachim Messing, Director The Waksman Institute of Microbiology (WIM) is an internationally recognized center of excellence in molecular genetics. Fifteen laboratories, arranged around three groups (microbial, plant, and animal developmental genetics) and equipped with state-of-the-art equipment, provide graduate students the opportunity to learn the latest techniques in molecular genetics. In addition, the Waksman Institute houses the Fermentation and Genomics Core Facilities to provide infrastructure to the larger research community. Research has enabled the members of the institute to push the frontiers of scientific knowledge to new levels from better nutrition to drug-resistance of infectious diseases, from cancer to birth defects ...
The conjugative S. ambofaciens integrative element pSAM2 possesses a kil-kor system. The korSA gene has been identified as a key element of this system, and thekil locus has been located in the region oftraSA, the main transfer gene (7, 25), but the direct role of KorSA in the regulation of the kil locus remained unknown. In this study we characterized the targets of the KorSA protein in the pSAM2 sequence.. Considering the relatively small size of the pSAM2 genome and the availability of its complete sequence, gel shift experiments were performed with the totality of the DNA fragments obtained after pSAM2 digestion with restriction endonucleases. Using this new approach, it was possible to demonstrate directly that KorSA binds only to the promoter regions of two pSAM2 genes, pra andkorSA, with no other binding sites detected. Unlike other known actinomycete mobile elements, KorSA did not bind either totraSA, the main pSAM2 transfer gene, or to thespdA, -B, -C, and -D genes involved in pSAM2 ...
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An illustrated identification guide was compiled and published for identification of Streptomyces genera covering 342 species of Streptomyces and related genera as: Mahesh S. Yandigeri, Dilip K. Arora and Arvind K. Yadav (2010) Synoptical Keys for Identification of Streptomyces Genera published by National Bureau of Agriculturally Important Microorganisms (NBAIM), Kushmaur, Mau Nath Bhanjan (U.P.), India (ISBN: 978-81-909892-0-6 ...
556 The pyrrolo[2,1-c][1,4]benzodiazepines (PBDs) are naturally occurring antitumor antibiotics isolated from various Streptomyces species. They bind covalently in the minor groove of DNA at purine-guanine-purine motifs. SJG-136, a pyrrolobenzodiazepine dimer based on two PBD units joined through their C8-positions via a propyldioxy linker, is presently in Phase I evaluation in both the UK (through Cancer Research UK) and the USA (through the NCI) as a result of its striking in vitro and in vivo activity. As it contains two electrophilic centers, SJG-136 forms interstrand cross-links between guanines on opposite strands of DNA at Purine-GATC-Pyrimidine sequences. More recently we have explored the synthesis and biological activity of C2-aryl substituted PBD monomers that monoalkylate guanines in the minor groove of DNA rather than form cross-links. In this respect, their mechanism of action is similar to Ecteinascidin-743 (Yondelis, ET-743). We have found that these molecules possess encouraging ...
This antibiotic originating from the Gram+ actinomycete Streptomyces venezulenza has about the same type of activity as cycloheximide, but than with an ...
Chloramphenicol is a broad-spectrum antibiotic that was derived from the bacterium Streptomyces venezuelae and is now produced synthetically. Chloramphenicol is effective against a wide variety of microorganisms, but due to serious side-effects (e.g., damage to the bone marrow, including aplastic anemia) in humans, it is usually reserved for the treatment of serious and life-threatening infections (e.g., typhoid fever). Chloramphenicol is bacteriostatic but may be bactericidal in high concentrations or when used against highly susceptible organisms. Chloramphenicol stops bacterial growth by binding to the bacterial ribosome (blocking peptidyl transferase) and inhibiting protein synthesis ...
Chloramphenicol is a broad-spectrum antibiotic that was derived from the bacterium Streptomyces venezuelae and is now produced synthetically. Chloramphenicol is effective against a wide variety of microorganisms, but due to serious side-effects (e.g., damage to the bone marrow, including aplastic anemia) in humans, it is usually reserved for the treatment of serious and life-threatening infections (e.g., typhoid fever). Chloramphenicol is bacteriostatic but may be bactericidal in high concentrations or when used against highly susceptible organisms. Chloramphenicol stops bacterial growth by binding to the bacterial ribosome (blocking peptidyl transferase) and inhibiting protein synthesis ...
Fu et al. reported the discovery of novel anticancer inducamides A-C from a marine derived Streptomyces sp. strain SNC109-M3 that was obtained through a selective mutation on the β subunit of RNA polymerase that stimulated inducamide production. Inducamide C exhibits the strongest anticancer properties. The goal of the current project is to characterize the genes required for inducamide production by Streptomyces. We determined the complete 9.8 million base-pair genome of SNC109-M3 in order to locate the hypothesized inducamide gene cluster. This revealed an approximately 18 kilobase-pair gene cluster encoding several proteins with hypothesized roles in inducamide biosynthesis. After construction of a clone library containing colonies of E. coli with 50 kilobase-pairs of randomly fragmented SNC109-M3 DNA ligated into a cosmid, PCR screening will be performed to locate the library member that contains the putative biosynthetic gene cluster. The gene cluster will be expressed in a heterologous ...
Two-component signal transduction systems enable bacteria to sense, respond, and adapt to changes in their environment or in their intracellular state. Each two-component system consists of a sensor protein-histidine kinase (HK) and a response regulator (RR). In the prototypical two-component pathway, the sensor HK phosphorylates its own conserved His residue in response to a signal(s) in the environment. Subsequently, the phosphoryl group of HK is transferred onto a specific Asp residue on the RR. The activated RR can then effect changes in cellular physiology, often by regulating gene expression. Two-component pathways thus often enable cells to sense and respond to stimuli by inducing changes in transcription ...
Novel compounds are produced by the fermentation of a nutrient medium with the previously undescribed microorganism Streptomyces avermitilis. They may be isolated by solvent extraction and chromatogra
The drug is an immunosuppressive agent. Sirolimus is a macrocyclic lactone produced by Streptomyces hygroscopicus. It is also commonly known as Rapamycin or Rapamune. It is used to prevent organ rejection (kidney transplants) but is also being used in clinical trials in combinations for the treatment of various cancers.
... Tacrolimus is a macrolide antibiotic, it acts by inhibiting cytokine production. It possess anti-inflammatory and immunomodulatory activities.Mechanism: Tacrolimus binds to FKBP 12 and forms a complex
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Streptomyces venezuelae decarboxylase JadN (jadN), JadX (jadX), NDP-hexose2,3-dehydratase (jadO), NDP-hexose 3-ketoreductase (jadP), NDP-glucosephosphate nucleotidyltransferase (jadQ), glycosyltransferase (jadS),NDP-hexose 4,6-dehydratase (jadT), NDP-4-keto-6-deoxyhexose 5-epimerase(jadU), NDP-4-keto-6-deoxyhexose 4-ketoreductase (jadV), JadR* (jadR*), andJadY (jadY) genes, complete cds; and JadZ (jadZ) gene, partial ...
I feel very proud of that picture, and not only because it took ages fiddling around with a microscope and two broken slides (dont ask!) to produce. The big blob in the middle is the Streptomyces, but its not one Streptomyces bacteria its a whole network of integrated hyphae; long branching filamentous cells. This can be seen particularly well in the largest blob in the middle, which has a filamentous tail like structure coming out of it, these are the mycelium branching out and breaking away. Some of them will branch off completely as single cells, and differentiate to form spores ...
Benastatins A and B, new inhibitors of glutathione S-transferase, produced by Streptomyces sp. MI384-DF12. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.:I. TAXONOMY, PRODUCTION, ISOLATION, PHYSICO-CHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES (1992 ...
Pesticidally active substantially pure nikkomicin fractions obtained from Streptomyces tendae Ettlinger et al Tu 901, the fractions being selected from the group consisting of ##STR1## a mixture of nikkomicin I and J, and a mixture of nikkomicin M and N.
This model represents the amino-terminal helix-turn-helix repressor region of the biotin--acetyl-CoA-carboxylase ligase/biotin operon repressor bifunctional protein BirA. In many species, the biotin--acetyl-CoA-carboxylase ligase ortholog lacks this DNA-binding repressor region and therefore is not equivalent to the well-characterized BirA of E. coli. This HMM may recognize some other putative repressor proteins, such as DnrO of Streptomyces peucetius with scores below the noise cutoff but with significance shown by low E-value ...
In addition to the potential implications for medicine (i.e., antibiotics+phages combination therapy), I like the thought-provoking idea of a possible co-evolution of certain traits in antibiotic-producing microbes and in phages infecting antibiotic-sensitive bacteria. One can imagine (have a look at Figure 1) several concentric, inhibition zones surrounding the cells (or mycelia) of an antibiotic producer. Inner zones, where antibiotic concentration is deadly for different microbes (depending on their respective sensitivities). And outer zones, where the antibiotic concentration is only sub-lethal but stimulate phage production in sensitive phage-microbe couples; we might picture it as a "defensive barrier" consisting of a higher local concentration of phages, ready to attack sensitive newcomers. Given that we really dont know the antibiotic concentrations that are actually produced by microorganisms in natural environments (at least I dont know), the sub-lethal effects of secondary ...
A macrolide isolated from the culture broth of a strain of Streptomyces tsukubaensis that has strong immunosuppressive activity in vivo and prevents the activation of T-lymphocytes in response to antigenic or mitogenic stimulation in vitro ...
(Phys.org) -- Streptomyces produce the majority of clinically useful antibiotics, yet we don’t fully understand how they grow. PhD student Antje Hempel has contributed to our understanding of this by working out how ...
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Not only do senior cititzens have the same rights in a nursing facility as they would in their own homes, but they also have trained, paid advocates ready to press for solutions on their behalf. Three community ombudsmen, along with 18 volunteers, work out of the regional long-term care ombudsman office.
A 0·5 kb fragment of Streptomyces venezuelae ISP5230 genomic DNA was amplified by PCR using primers based on consensus sequences of cysteine synthase isozyme A from bacteria. The deduced amino acid sequence of the PCR product resembled not only cysteine synthase sequences from prokaryotes and eukaryotes but also eukaryotic cystathionine β-synthase sequences. Probing an Str. venezuelae genomic library with the PCR product located a hybridizing colony from which pJV207 was isolated. Sequencing and analysis of the Str. venezuelae DNA insert in pJV207 detected two ORFs. The deduced amino acid sequence of ORF1 matched both cysteine synthase and cystathionine β-synthase sequences in GenBank, but its size favoured assignment as a cystathionine β-synthase. ORF2 in the pJV207 insert was unrelated in function to ORF1; in its sequence the deduced product resembled acetyl-CoA transferases, but disruption of the ORF did not cause a detectable phenotypic change. Disruption of ORF1 failed to elicit cysteine
Streptomyces species are considered exceptionally well endowed for chemical warfare, presumably allowing them to eliminate bacterial and fungal competitors in soil ecosystems. This was first realized over fifty years ago, and since then the majority of known antibiotics have been isolated from Streptomyces. Although thousands of antibiotics have been described, these are thought to represent only a small fraction of the repertoire of bioactive compounds produced by Streptomyces [4,5]. In addition, empirical screening using various assays has revealed that Streptomyces culture supernatants contain other pharmaceutically active compounds, such as anti-viral and anti-cancer compounds, modulators of immune responses, and various enzyme inhibitors, as well as herbicides, insecticides, and anti-parasitic compounds [4,6,7]. Furthermore, chemical screening methods have uncovered the remarkable structural diversity of these compounds. Genome sequences of Streptomyces species are now providing an ...
Detail záznamu - tmRNA of Streptomyces collinus and Streptomyces griseus during the growth and in the presence of antibiotics - Detail záznamu - Knihovna Akademie věd České republiky
Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology
To improve the fermentation production of transglutaminase (TGase) from Streptomyces mobaraensis for applications in the food industry, the atmospheric and room-temperature plasma (ARTP) mutagenesis was applied to breed S. mobaraensis mutants with increased TGase production. After eight rounds of iterative ARTP mutagenesis, four genetically stable mutants, Sm5-V1, Sm6-V13, Sm2-V10, and Sm7-V12, were identified, which showed increased TGase production by 27, 24, 24, and 19%, respectively. The best mutant Sm5-V1 exhibited a maximum TGase activity of 5.85 U/mL during flask fermentation. Compared to the wild-type strain, the transcription levels of the zymogen TGase genes in the mutants increased significantly as indicated by quantitative real-time PCR, while the gene nucleotide sequences of the mutants did not change at all. It was shown that the overexpression of TGase zymogen gene in the mutants contributes to the increase in TGase production. ARTP is a potentially efficient tool for microbial mutation
TY - JOUR. T1 - TrpM, a Small Protein Modulating Tryptophan Biosynthesis and Morpho-Physiological Differentiation in Streptomyces coelicolor A3(2). AU - Puglia, Anna Maria. AU - Botta, Luigi. AU - Giardina, Anna. AU - Gallo, Giuseppe. AU - Sutera, Alberto. AU - Palazzotto, Emilia. AU - Scaloni, Andrea. AU - Renzone, Giovanni. AU - Palazzotto, Emilia. PY - 2016. Y1 - 2016. N2 - In the model actinomycete Streptomyces coelicolor A3(2), small open reading frames encoding proteins with unknown functions were identified in several amino acid biosynthetic gene operons, such as SCO2038 (trpX) in the tryptophan trpCXBA locus. In this study, the role of the corresponding protein in tryptophan biosynthesis was investigated by combining phenotypic and molecular analyses. The 2038KO mutant strain was characterized by delayed growth, smaller aerial hyphae and reduced production of spores and actinorhodin antibiotic, with respect to the WT strain. The capability of this mutant to grow on minimal medium was ...
Self-resistance of the nourseothricin-producing strain Streptomyces noursei.: The nourseothricin producer Streptomyces noursei is resistant to its own antibioti
Malate synthases (MS) from Streptomyces coelicolor A3(2) and S. clavuligerus NRRL3585 were cloned by polymerase chain reaction into a glutathione S-transferase (GST) fusion expression vector and heterologously expressed in Escherichia coli. The fusion GST-MS construct improved the soluble expression of MS by approximately 10-fold compared to the soluble expression of nonfusion MS. With the significant improvement in levels of soluble MS, purification and subsequent cleavage of recombinant MS from GST were facilitated in this study. Using purified enzymes, optimized parameters, which achieved maximal specific activity, were established in the enzymatic assay for streptomycete MS. The average purified specific activities of S. coelicolorand S. clavuligerus MS were 26199 and 11821 nmol/mg min, respectively. Furthermore, enzymatic analysis revealed that the two streptomycete MS displayed a similar Km value for acetyl-CoA, but S. coelicolor MS had a Km value for glyoxylate that is approximately ...
Amicetin, an antibacterial and antiviral agent, belongs to a group of disaccharide nucleoside antibiotics featuring an alpha-(1 -> 4)-glycoside bond in the disaccharide moiety. In this study, the amicetin biosynthesis gene cluster was cloned from Streptomyces vinaceusdrappus NRRL 2363 and localized on a 37-kb contiguous DNA region. Heterologous expression of the amicetin biosynthesis gene cluster in Streptomyces lividans TK64 resulted in the production of amicetin and its analogues, thereby confirming the identity of the ami gene cluster. In silico sequence analysis revealed that 21 genes were putatively involved in amicetin biosynthesis, including 3 for regulation and transportation, 10 for disaccharide biosynthesis, and 8 for the formation of the amicetin skeleton by the linkage of cytosine, p-aminobenzoic acid (PABA), and the terminal (+)-alpha-methylserine moieties. The inactivation of the benzoate coenzyme A (benzoate-CoA) ligase gene amiL and the N-acetyltransferase gene amiF led to two ...
Organofluorine compounds are widely prepared throughout the chemicals industry, but their prepararion generally requires harsh fluorinating reagents and non-aqueous solvents. On the other hand, biology has hardly exploited organofluorine compounds. A very few organisms synthesize organofluorine metabolites, suggesting they have evolved a mechanism to overcome the kinetic desolvation barrier to utilizing F(-)(aq). Here, the purification and crystallization of an enzyme from Streptomyces cattleya which is responsible for the synthesis of the C-F bond during fluoroacetate and 4-fluorothreonine biosynthesis is reported. The protein crystallizes in space group C222(1), with unit-cell parameters a = 75.9, b = 130.3, c = 183.4 A, alpha = beta = gamma = 90 degrees. Data were recorded to 1.9 A at the ESRF. The structure of the protein should provide important insights into the biochemical process of C-F bond formation.. ...
g- 1, 6 NAKAO ISHIDA ETAL 3,334,022 NEOCARZINOSTATIN PRODUCED BY STREPTOMYCES CARZINOSTATICUS VAR NEOCARZINOSTATICUS Filed April 26, 1965 2 Sheets-Sheet 1 NAKAO ISHIDA KEIZO MIYAZAKI KATSUO KUMAGAI MITSUO RIKIMARU MA SAl-HKO KUROYA INVENTORS. B. SIMONTON & HERBERT W TAYLOR,JR. ATTORNEYS. NAKAO-ISHIDA ETAL NEOCARZINOSTATIN PRODUCED BY STREPTOMYCES Aug. l 1967 3,334,022 NOSTATICUS VAR. NEOCARZINOSIATICUS v CARZI Filed April 26, 1965 2 Sheets-Sheet O O O O O O O om D P a f P Om \r fl cm 09 PERCENT TRANSMISSION IUA IAR KGAW AA M WK W Y IKRO OO O UUH AO A QTT AEA NKKMM N w w 2% K ON N R IOYO M T ABIT H WA W RWR TMEK R BR CRRH Y B United States Patent 3,334,022 NEOCARZINOSTATIN PRODUCED BY STREPTOMYCES CARZIZVOSTATIC US VAR. NEOCARZINOSTATICUS Nakao Ishida, 90 Kakogoro-cho; Keizo Miyazaki, 48 Kakyoin-dori; Katsuo Kumagai, 129 Tori-cho; and Mitsuo Rikimaru, 61 Kita-shichiban-cho, all of Sendai, Japan; and Masahiko Kuroya, Room 605, Empire Corp., 28 Daikyo-machi, Shinjuku-ku, Tokyo, Japan Filed Apr. ...
A homologue of the bacterial cell division gene ftsZ was cloned from the filamentous bacterium Streptomyces coelicolor. The gene was located on the physical map of the chromosome at about 11 oclock (in the vicinity of glkA, hisA and trpB). Surprisingly, a null mutant in which the 399-codon ftsZ open reading frame was largely deleted was viable, even though the mutant was blocked in septum formation. This indicates that cell division may not be essential for the growth and viability of S. coelicolor. The ftsZ mutant was able to produce aerial hyphae but was unable to produce spores, a finding consistent with the idea that ftsZ is required in order for aerial hyphae to undergo septation into the uninucleoid cells that differentiate into spores. ...
Cell division in the Gram-positive bacterium Streptomyces coelicolor starts with the assembly of the tubulin homologue FtsZ into a cytokinetic ring (the Z ring) at the site of septation. In stark contrast to the binary fission of most bacteria, the syncytial hyphal cells of S. coelicolor exploit two types of cell division with strikingly different outcomes depending on the developmental stage. The main goal of this study has been to identify developmental mechanisms that modulate this differential performance of the basic cell division machinery.. By isolation and characterization of a non-sporulating ftsZ mutant, we demonstrated that the requirements for Z-ring formation differ between the two types of septation. The ftsZ17(Spo) mutation abolished septation without overtly affecting vegetative growth. This mutant was defective in the assembly of FtsZ into regularly spaced Z rings in sporogenic hyphae, suggesting that the assembly of Z rings is developmentally controlled during ...