The crystal structures of truncated forms of the Streptococcus pyogenes phage-encoded hyaluronate lyases HylP2 and HylP3 were determined by molecular replacement to 1.6 and 1.9 Å resolution, respectively. The truncated forms crystallized in a hexagonal space group, forming a trimer around the threefold crystallographic axis. The arrangement of the fold is very similar to that observed in the structure of the related hyaluronate lyase HylP1. The structural elements putatively involved in substrate recognition are found to be conserved in both the HylP2 and HylP3 fragments.. ...
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Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.
Streptococcus pyogenes adalah penyebab banyak penyakit penting pada manusia yang berkisar dari infeksi kulit permukaan yang ringan hingga penyakit sistemik yang mengancam hidup. Infeksi khasnya bermula di tenggorokan atau kulit. Infeksi ringan Streptococcus pyogenes termasuk faringitis ("radang kerongkongan") dan infeksi kulit setempat ("impetigo"). Erisipelas dan selulitis dicirikan oleh perbiakan dan penyebaran samping Streptococcus pyogenes di lapisan dalam kulit. Serangan dan perbiakan Streptococcus pyogenes di fasia dapat menimbulkan fasitis nekrosis, keadaan yang besar kemungkinan mengancam hidup yang memerlukan penanganan bedah.. Infeksi akibat strain tertentu Streptococcus pyogenes bisa dikaitkan dengan pelepasan toksin bakteri. Infeksi kerongkongan yang dihubungkan dengan pelepasan toksin tertentu bisa menimbulkan penyakit jengkering (scarlet fever). Infeksi toksigen Streptococcus pyogenes lainnya bisa menimbulkan sindrom syok toksik streptococcus, yang bisa mengancam ...
Global Markets Directs Pharmaceutical and Healthcare latest pipeline guide Streptococcus pyogenes Infections - Pipeline Review, H2 2017, provides comprehensive information on the therapeutics under development for Streptococcus pyogenes Infections (Infectious Disease), complete with analysis by stage of development, drug target, mechanism of action (MoA), route of administration (RoA) and molecule type. The guide covers the descriptive pharmacological action of the therapeutics, its complete research and development history and latest news and press releases.. The Streptococcus pyogenes Infections (Infectious Disease) pipeline guide also reviews of key players involved in therapeutic development for Streptococcus pyogenes Infections and features dormant and discontinued projects. The guide covers therapeutics under Development by Companies /Universities /Institutes, the molecules developed by Companies in Phase II, Preclinical and Discovery stages are 1, 4 and 3 respectively. Similarly, the ...
As part of a Europe-wide initiative to explore current epidemiologic patterns of severe disease caused by Streptococcus pyogenes, the United Kingdom undertook enhanced population-based surveillance during 2003-2004. A total of 3,775 confirmed cases of severe S. pyogenes infection were identified over 2 years, 3.33/100,000 population, substantially more than previously estimated. Skin/soft tissue infections were the most common manifestation (42%), followed by respiratory tract infections (17%). Injection drug use was identified as a risk factor for 20% of case-patients. One in 5 infected case-patients died within 7 days of diagnosis; the highest mortality rate was for cases of necrotizing fasciitis (34%). Nonsteroidal antiinflammatory drugs, alcoholism, young age, and infection with emm/M3 types were independently associated with increased risk for streptococcal toxic shock syndrome. Understanding the pattern of these diseases and predictors of poor patient outcome will help with identification and
A vaccine to protect against diseases resulting from infection with Streptococcus pyogenes is under development. This vaccine utilizes a recombinant fusion protein (SpeAB) comprising of genetically detoxified SpeA, a secreted toxin, and SpeB, a surface bound and secreted cysteine protease. During phase I investigations a lead vaccine formulation was determined by optimization of critical parameters such as buffer, pH, stabilizer, and adjuvant interactions. Potency studies in mice demonstrated significantly greater induction of an antigen-specific IgG immune response to the vaccine than antigen alone. Additionally, the antibodies produced were functional at neutralization of wild type SpeA toxin. These results demonstrate that the SpeAB vaccine developed during the phase I investigation has great potential to provide protection against diseases resulting from infection by Streptococcus pyogenes. For phase II development of the vaccine the overall goal is to complete non-clinical safety studies in ...
Streptococcal pyrogenic exotoxin B (SPE B), a conserved extracellular cysteine protease expressed by the human pathogenic bacterium Streptococcus pyogenes, was purified and shown to cleave inactive human interleukin 1 beta precursor (pIL-1 beta) to produce biologically active IL-1 beta. SPE B cleaves pIL-1 beta one residue amino-terminal to the site where a recently characterized endogenous human cysteine protease acts. IL-1 beta resulting from cleavage of pIL-1 beta by SPE B induced nitric oxide synthase activity in vascular smooth muscle cells and killed of the human melanoma A375 line. Two additional naturally occurring SPE B variants cleaved pIL-1 beta in a similar fashion. By demonstrating that SPE B catalyzes the formation of biologically active IL-1 beta from inactive pIL-1 beta, our data add a further dimension to an emerging theme in microbial pathogenesis that bacterial and viral virulence factors act directly on host cytokine pathways. The data also contribute to an enlarging ...
phdthesis{4c19e931-9bbd-4b30-a22f-1c1c93a81582, abstract = {Several bacterial species express surface proteins with affinity for the Fc part of human IgG. This thesis describes evolutionary and functional studies of protein H, an IgGFc-binding surface protein of the human pathogen Streptococcus pyogenes. By using peptide mapping and NMR analysis the IgGFc-binding region of protein G, a surface protein of group C and G streptococci, was identified. Various fragments of protein H localized the IgGFc-binding region to the N-terminal part of this molecule. It was also demonstrated that protein A, an IgGFc-binding protein of Staphylococcus aureus, protein G and protein H bind to the same site on IgGFc, although there is no sequence homology between the IgGFc-binding regions of these proteins. The finding that unrelated bacterial proteins have evolved regions with similar function, represents an example of convergent evolution. Protein H also absorbs albumin from human plasma and by using fragments of ...
TY - JOUR. T1 - Structure and Function Characterization of the a1a2 Motifs of Streptococcus pyogenes M Protein in Human Plasminogen Binding. AU - Quek, Adam J.H.. AU - Mazzitelli, Blake A.. AU - Wu, Guojie. AU - Leung, Eleanor W.W.. AU - Caradoc-Davies, Tom T.. AU - Lloyd, Gordon J.. AU - Jeevarajah, Devadharshini. AU - Conroy, Paul J.. AU - Sanderson-Smith, Martina. AU - Yuan, Yue. AU - Ayinuola, Yetunde A.. AU - Castellino, Francis J.. AU - Whisstock, James C.. AU - Law, Ruby H.P.. PY - 2019/9/6. Y1 - 2019/9/6. N2 - Plasminogen (Plg)-binding M protein (PAM) is a group A streptococcal cell surface receptor that is crucial for bacterial virulence. Previous studies revealed that, by binding to the kringle 2 (KR2) domain of host Plg, the pathogen attains a proteolytic microenvironment on the cell surface that facilitates its dissemination from the primary infection site. Each of the PAM molecules in their dimeric assembly consists of two Plg binding motifs (called the a1 and a2 repeats). To date, ...
HTF Market Intelligence released a new research report of 46 pages on title Streptococcus pyogenes Infections - Pipeline Review, H2 2016 with de
A better understanding of transcriptional and post-transcriptional regulation of gene expression in bacteria relies on studying their transcriptome. RNA sequencing methods are used not only to assess RNA abundance but also the exact boundaries of primary and processed transcripts. Here, we developed a method, called identification of specific cleavage position (ISCP), which enables the identification of direct endoribonuclease targets in vivo by comparing the 5 and 3 ends of processed transcripts between wild type and RNase deficient strains. To demonstrate the ISCP method, we used as a model the double-stranded specific RNase III in the human pathogen Streptococcus pyogenes. We mapped 92 specific cleavage positions (SCPs) among which, 48 were previously described and 44 are new, with the characteristic 2 nucleotides 3 overhang of RNase III. Most SCPs were located in untranslated regions of RNAs. We screened for RNase III targets using transcriptomic differential expression analysis (DEA) and ...
TY - JOUR. T1 - Emergence of fluoroquinolone-resistant Streptococcus pyogenes in Japan by a point mutation leading to a new amino acid substitution. AU - Arai, Kazuaki. AU - Hirakata, Yoichi. AU - Yano, Hisakazu. AU - Kanamori, Hajime. AU - Endo, Shiro. AU - Hirotani, Ayako. AU - Abe, Yuko. AU - Nagasawa, Mitsuaki. AU - Kitagawa, Miho. AU - Aoyagi, Tetsuji. AU - Hatta, Masumitsu. AU - Yamada, Mitsuhiro. AU - Nishimaki, Katsushi. AU - Takayama, Yoko. AU - Yamamoto, Natsuo. AU - Kunishima, Hiroyuki. AU - Kaku, Mitsuo. PY - 2011/3/1. Y1 - 2011/3/1. N2 - Objectives: Streptococcus pyogenes causes various diseases in humans. While the prevalence of fluoroquinolone-resistant S. pyogenes isolates has been increasing since 2000 in the USA and Europe, it has remained very low in Japan. We isolated a fluoroquinolone-resistant S. pyogenes strain and analysed its genetics. Methods: TU-296, a strain of S. pyogenes resistant to levofloxacin (MIC 16 mg/L), was isolated from the throat of a patient in their ...
Baldassarri L, Creti R, Recchia S, Imperi M, Facinelli B, Giovanetti E, Pataracchia M, Alfarone G & Orefic G (2006) Therapeutic failures of antibiotics used to treat macrolide-susceptible Streptococcus pyogenes infections may be due to biofilm formation. J Clin Microbiol 44: 2721-2727 ...
Estimates of disease burden and data on the sources of invasive postpartum group A streptococcus (GAS) infections will help guide public health action. Active, population-based surveillance was conducted in 9 regions from 1995 through 2000. A case of GAS infection was defined as isolation of GAS from a sterile site in a resident of a surveillance area who was pregnant or in the postpartum period. Census and live birth data were used to calculate rates. Eighty-seven cases of postpartum GAS infection (2.2% of 3957 invasive GAS infections) occurred at 3%-8% of hospitals annually. We estimate that 220 cases occurred annually in the United States. Two or more cases were noted during 6 months at 8 hospitals, during 1 year at 13 hospitals, and during 2 years at 16 hospitals. Cases due to identical emm types clustered more frequently than expected by chance. Although postpartum GAS infections are rare, the clustering of infections due to identical strains suggests that some invasive cases may have a ...
Culture of Streptococcus pyogenes (group A streptococci) on blood agar. Streptococcus pyogenes can colonize the upper respiratory tract without signs of disease, but generally it is a pathogen of humans with a wide range of diseases. Most common is the inflammation of the pharynx but it can also cause life-threatening infections such as skin or soft-tissue infections. - Stock Image C029/1367
SALDIAS P, Fernando; YANEZ V, Jorge; SALDIAS H, Velia y DIAZ P, Orlando. Community-acquired pneumonia caused by Streptococcus pyogenes: Report of one case. Rev. méd. Chile [online]. 2008, vol.136, n.12, pp.1564-1569. ISSN 0034-9887. http://dx.doi.org/10.4067/S0034-98872008001200009.. During the past two decades there has been a resurgence ofinvasive group A streptococcal (GAS) infection, specially pneumonia and bacteremia. We report a 35 year-old female previously subjected to a thyroidectomy for a thyroid cáncer, that five days after operation, presented with a severe community-acquired pneumonia caused by Streptococcus pyogenes (Lancefield Group A Streptococcus) that was complicated by acute respiratory failure and septic shock. She was treated with a combination of 3 g/day of cefotaxime and 1.8 g/day of clindamycin with a good clinical response and discharged from the hospital in good conditions. Although this microorganism is an uncommon cause of community-acquired pneumonia, previously ...
SALDIAS P, Fernando; YANEZ V, Jorge; SALDIAS H, Velia y DIAZ P, Orlando. Community-acquired pneumonia caused by Streptococcus pyogenes: Report of one case. Rev. méd. Chile [online]. 2008, vol.136, n.12, pp.1564-1569. ISSN 0034-9887. http://dx.doi.org/10.4067/S0034-98872008001200009.. During the past two decades there has been a resurgence ofinvasive group A streptococcal (GAS) infection, specially pneumonia and bacteremia. We report a 35 year-old female previously subjected to a thyroidectomy for a thyroid cáncer, that five days after operation, presented with a severe community-acquired pneumonia caused by Streptococcus pyogenes (Lancefield Group A Streptococcus) that was complicated by acute respiratory failure and septic shock. She was treated with a combination of 3 g/day of cefotaxime and 1.8 g/day of clindamycin with a good clinical response and discharged from the hospital in good conditions. Although this microorganism is an uncommon cause of community-acquired pneumonia, previously ...
Wounds are known to serve as portals of entry for group A Streptococcus (GAS). Subsequent tissue colonization is mediated by interactions between GAS surface proteins and host extracellular matrix components. We recently reported that the streptococcal collagen-like protein-1, Scl1, selectively binds the cellular form of fibronectin (cFn) and also contributes to GAS biofilm formation on abiotic su
In the Streptococcus pyogenes M6 strain D471, an insertion of the conjugative transposon Tn916 into a region 2 kb upstream of the promoter of emm6 (the structural gene for the M protein) rendered the strain M negative (M. G. Caparon and J. R. Scott, Proc. Natl. Acad. Sci. USA 84:8677-8681, 1987). In the present work, we show that this insertion mutation, mry-1, is 244 bp upstream of an open reading frame encoding a protein we call Mry. This protein is visible on a gel after transcription and translation in vitro. We have developed a technique for complementation analysis in S. pyogenes and have used it to show that the wild-type mry gene is dominant to two mutant alleles. This dominance indicates that Mry acts in trans as a positive regulator of the emm6 gene. The translated DNA sequence of mry has two regions of similarity to the motif common to the receptor protein of two-component regulatory systems. In addition, the N terminus of Mry has two regions resembling a helix-turn-helix motif. Mry ...
The pathogen Streptococcus pyogenes is a significant cause of human morbidity and mortality. Most of the work in this thesis is focused on streptococcal virulence factor IdeS, but the thesis also features work on SpeB, another streptococcal virulence factor. Both IdeS and SpeB are secreted cysteine proteases and both have previously been shown to degrade human IgG. IgG is the only known substrate for IdeS while SpeB is a more promiscuous protease with a larger number of identified substrates. A significant part of the data presented in this thesis is the result of designing and optimizing methods to detect and accurately measure the proteolytic degradation of IgG. Methods aimed at measuring the binding interactions between enzyme and substrate have also been frequently utilized. I show that IdeS is a monomeric protease, as opposed to previously published data that suggested it to be dimeric. IdeS cleaves the two heavy chains of IgG in a two-step reaction and I demonstrate that the first cleavage ...
Streptococcus pyogenes GRAB protein: GRAB - G-related alpha2-macroglobulin-binding protein; isolated from Streptococcus pyogenes; amino acid sequence in first source
Streptococcus pyogenes ATCC ® BAA-1315D-5™ Designation: Genomic from Streptococcus pyogenes MGAS9429 (ATCC BAA-1315) TypeStrain=False Application:
Streptococcus pyogenes ATCC ® 12344D-5™ Designation: Genomic DNA from Streptococcus pyogenes typing strain T1 TypeStrain=True Application: Emerging infectious disease research
Streptococcus pyogenes: | | | | |Streptococcus pyogenes| | | ... World Heritage Encyclopedia, the aggregation of the largest online encyclopedias available, and the most definitive collection ever assembled.
Fig. 4. Analysis of the M protein of the wild type and thesagA mutant. (A) Schematic diagram of the structure of the M6 protein indicating the location of the epitopes recognized by the antibodies used. Repeat regions are shown by capital letters and the cell-associated region and cell wall anchoring signals are indicated. (B and C) Western blot analysis of whole-cell extracts separated by SDS-10% (B) or 4 to 12% (C) PAGE. The antibodies used are indicated below the gels. Lanes contain extracts from JRS4 (lanes 1), JRS470 (lanes 2), and JRS145 (lanes 3). M, Rainbow (B; Amersham) or See Blue (C; Invitrogen) molecular mass marker. Bands that reacted with anti-M antibody are indicated by arrows. ...
So when nine patients contracted Streptococcus pyogenes infections during the month of august, there was a serious cause for concern. These patients had very little in common. They were operated on in different theatres. they were housed in different wards. In fact the only thing linking these patients was the anaesthetist attending them. He hadnt been ill with any of the disease associated with Streptococcus pyogenes. But it is possible for people to be asymptomatically colonised on their throats. So naturally they took throat swabs from him. They found nothing. There was no trace of Streptococcus pyogenes. So he couldnt possibly be the source of the infection ...
This thesis investigates the transcriptional responses of Streptococcus pyogenes in the pharyngeal environment and characterizes two transcriptional regulators involved in the adaptive response to the host. Furthermore, this thesis explores the potential role of integrated prophage on the streptococcal transcriptome. We are specifically interested in global regulatory systems in group A streptococci and how they impact virulence regulation. With this work, we hoped to identify new regulatory elements involved in the infection process. The first objective of this thesis was to determine the transcriptional shift induced in streptococci upon introduction into the in vitro host environment. Using three time points, we examined the dynamic transcriptome remodeling program that streptococci undergo following exposure to cell-free pharyngeal culture supernatants or during co-culture with intact pharyngeal monolayers. These studies highlighted that streptococci modulate expression of virulence factors in the
I love fallacies, old wives tales, and urban myths…. You are probably familiar with the dogma that you should never use cotrimoxazole to treat infections due to Streptococcus pyogenes.. In the laboratory setting we have traditionally never been much good at in-vitro susceptibility testing for cotrimoxazole against Streptococcus pyogenes…. Why?. In vitro susceptibility testing of Streptococcus pyogenes against cotrimoxazole is dependent on/vulnerable to the amount of thymidine in the susceptibility media. Thymidine allows Streptococcus pyogenes to bypass sulphonamide mediated inhibition of folate metabolism.. In the past, media contained unregulated (and often high) levels of thymidine, particularly those media that contained blood. As a consequence Streptococcus pyogenes survived quite happily on such media, even in the presence of sulphonamides and thus showed in-vitro resistance.. However modern media such as Mueller-Hinton agar (MHA) are now regulated as to their thymidine content. A ...
Intravenous injection of Type 1 streptococcal M protein into mice and rats produced lesions confined to renal glomeruli. Thrombi of eosinophilic amorphous material, seen to occlude glomerular capillaries, were shown to contain M protein and fibrinogen. Gradual regression of the morphological lesions was observed during the 3 weeks following injection. Initial abnormal proteinuria and azotemia returned to control levels by the end of the 1st week; a second rise in urinary protein excretion and urea retention was demonstrated in some rats coincident with appearance of anti-M antibodies. The mechanism of renal localization of streptococcal M protein by means of a complex with fibrinogen was suggested, which may comprise an initial phase in the pathogenesis of acute poststreptococcal glomerulonephritis.. ...
Streptococcus pyogenes, (group A streptococcus; GAS) is a Gram-positive bacterial pathogene that is responsible for a number of suppurative infections and non-suppurative sequelae. Here we report the complete genome sequence of S. pyogenes serotype M3 strain SSI-1, isolated from a TSLS patient in Japna, and compared it with those of other GAS serotypes ...
Todars Online Textbook of Bacteriology chapter on Streptococcus pyogenes discusses Group A strep, GAS, strep throat and other streptococcal diseases.
This thesis focuses on host and pathogen specific interactions during invasive disease. We have investigated the role and impact of different virulence factors of Neisseria gonorrhoeae, N. meningitidis and Streptococcus pyogenes on host epithelial cells and in vivo. N. gonorrhoeae cause the sexually transmitted disease gonorrhoea and N. meningitidis is the most common cause of bacterial meningitis and may be leathal to the host within hours of infection. The neisserial type IV pili were shown to have an important impact on host cells for the induction of pro-inflammatory and other cellular defence transcriptional responses. Furthermore, N. meningitidis generally induced an earlier response compared to N. gonorrhoeae, probably as a result of the meningococcal capsule. The role of N. meningitidis serogroup B lipooliogsaccharide was investigated during invasive disease. Bacterial invasion of host cells and blood survival as well as virulence in vivo was dependent on the integrity of the LOS ...
phdthesis{e0687830-50ba-47d3-97d5-1bd517693461, abstract = {Cell wall-attached proteins (CWPs) are important for the virulence of Gram-positive bacteria, but are also targets for the host immune system. CWPs have a conserved COOH-terminal region, responsible for cell wall-sorting. A pattern based on this conserved region was developed. The pattern identifies genes encoding CWPs, with high sensitivity and specificity, in whole genomes of Gram-positive bacteria. In genomes of five Gram-positive bacterial species, 19 previously unknown putative CWPs were identified.,br/,,br, ,br/,,br, Three novel CWPs were identified and characterised in <i>Streptococcus pyogenes</i>, an important Gram-positive human pathogen. One CWP, GRAB, binds the human proteinase inhibitor alpha<sub>2</sub>-macroglobulin to the <i>S. pyogenes</i> surface, thus protecting other CWPs from proteolysis. Moreover, two novel CWPs (SclA and SclB) with regions similar to collagen were studied. These ...
1 Invasive Group A Strep Infections Associated With Bath Salts Injection Patty Carson, BA, SM (ASCP) 1,2, Sara Robinson, MPH 1, Stephen Sears, MD, MPH 1 1 Maine Center for Disease Control and Prevention; 2 University of Southern Maine Streptococcus pyogenes, or Group A Strep (GAS), are common bacteria in the throat and on the skin. These bacteria can cause Strep throat or noninvasive skin infections such as impetigo and cellulitis, and can cause invasive disease such as bloodstream infections and necrotizing fasciitis. Invasive disease can be fatal. Streptococcal toxic shock syndrome (STSS) can be associated with invasive or noninvasive GAS infection. STSS can be diagnosed when a patient has GAS infection, as well as hypotension and at least two other clinical findings consistent with more serious organ involvement such as renal impairment, coagulopathy, liver damage indicators, and tissue necrosis. In late 2012, four cases of invasive GAS were reported to the Maine Center for Disease Control ...
Most invasive bacterial infections are caused by species that more commonly colonize the human host with minimal symptoms. Although phenotypic or genetic correlates underlying a bacteriums shift to enhanced virulence have been studied, the in vivo selection pressures governing such shifts are poorly understood. The globally disseminated M1T1 clone of group A Streptococcus (GAS) is linked with the rare but life-threatening syndromes of necrotizing fasciitis and toxic shock syndrome. Mutations in the GAS control of virulence regulatory sensor kinase (covRS) operon are associated with severe invasive disease, abolishing expression of a broad-spectrum cysteine protease (SpeB) and allowing the recruitment and activation of host plasminogen on the bacterial surface. Here we describe how bacteriophage-encoded GAS DNase (Sda1), which facilitates the pathogens escape from neutrophil extracellular traps, serves as a selective force for covRS mutation. The results provide a paradigm whereby natural ...
Methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pyogenes (group A streptococcus [GrAS]) cause serious and sometimes fatal human diseases. They are among the many Gram-positive pathogens for which resistance to leading antibiotics has emerged. As a result, alternative therapies n …
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|71910358|ref|YP_281908.1| from Streptococcus pyogenes MGAS5005. Plus protein sequence and external database links.
Streptococcus pyogenes, 0.1 mg. Streptococci form part of the normal human flora that resides on the skin, and can also colonize the respiratory, gastrointestinal, and genitourinary tracts.
Transcription of the surface-associated virulence factors of the group A streptococcus (GAS) Streptococcus pyogenes, M protein (emm) and the C5a peptidase (scpA), is activated by a protein called Mga (formerly Mry or VirR). To determine whether Mga binds directly to the promoters of the genes it regulates, a protein resulting from the fusion of Mga to the C-terminal end of maltose-binding protein was purified from Escherichia coli. Specific binding to the promoter regions of the scpA and emm alleles of the type M6 GAS strain JRS4 was demonstrated by electrophoresis of the DNA-protein complex. Competition studies showed that the region upstream of scpA bound MBP-Mga with a slightly higher affinity than did the region upstream of emm. DNase I protection experiments identified a single 45-bp binding site immediately upstream of and overlapping the -35 region of both promoters. Sequences homologous to the protected regions were found in the promoters of many emm, scp, and emm-like genes from strains ...
Streptococcus pyogenes is an important human pathogen and surface structures allow it to adhere to, colonize and invade the human host. Proteins containing leucine rich repeats (LRR) have been identified in mammals, viruses, archaea and several bacterial species. The LRRs are often involved in protein-protein interaction, are typically 20-30 amino acids long and the defining feature of the LRR motif is an 11-residue sequence LxxLxLxxNxL (x being any amino acid). The streptococcal leucine rich (Slr) protein is a hypothetical lipoprotein that has been shown to be involved in virulence, but at present no ligands for Slr have been identified. We could establish that Slr is a membrane attached horseshoe shaped lipoprotein by homology modeling, signal peptidase II inhibition, electron microscopy (of bacteria and purified protein) and immunoblotting. Based on our previous knowledge of LRR proteins we hypothesized that Slr could mediate binding to collagen. We could show by surface plasmon resonance ...
Life Sciences ; Biochemistry ; Glycobiology ; Molecular biophysics (biochemistry) ; Infectious diseases ; Immunology ; NMR spectroscopy ; Protein chemistry ; Enzymes ; Crystallography ; Biophysics ; Mass spectrometry ; Streptococcus pyogenes ; structural biology ; EndoS ; IdeS ; IgG ; antibodies
Antibiotic resistance pattern of streptococcus pyogenes isolated from clinical samples with special reference to quinolone resistance-IJMR-Print ISSN No:-2394-546X Online ISSN No:-2394-5478Article DOI No:-10.18231,Indian Journal of Microbiology Research-IP Innovative Publication Pvt Limited, Medical Journals Publicat
BioAssay record AID 385108 submitted by ChEMBL: Antibacterial activity against Streptococcus pyogenes ATCC 12344 in Mueller Hinton agar medium.
Streptococcal inhibitor of complement (Sic) is a highly polymorphic extracellular protein made by serotype M1 group A Streptococcus strains that contributes to bacterial persistence in the mammalian upper respiratory tract. New variants of the Sic protein arise very rapidly by positive selection in human populations during M1 epidemics. The human antibody response to Sic was analyzed. Of 636 persons living in diverse localities, 43% had anti-Sic serum antibodies, but only 16.4% had anti-M1 protein serum antibody. Anti-Sic antibody was also present in nasal wash specimens in high frequency. Linear B cell epitope mapping showed that serum antibodies recognized epitopes located in structurally variable regions of Sic and the amino terminal hypervariable region of the M1 protein. Phage display analyses confirmed that the polymorphic regions of Sic are primary targets of host antibodies. These results support the hypothesis that selection of Sic variants occurs on mucosal surfaces by a mechanism that ...
Streptococcus pyogenes is a major bacterial pathogen and a potent inducer of inflammation causing plasma leakage at the site of infection.
COGEM released a comprehensive database of pathogenicity assessment of around 2575 bacterial species in 2011. The database ranks the pathogenicity of species on a scale of 1 to 4. Streptococcus pyogenes ranks 2 on this scale: Species that can cause diseases in humans or animals, which are unlikely to spread in the human population and for which an adequate prophylaxis or therapy ...
The details of bibliography - Extensive diversity of streptococcus pyogenes in a remote human population reflects global-scale transmission rather than localised diversification
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Streptococcus pyogenes models created using SEM images as reference material. Models are scaled at 250,000X. A variety of models of different bacterial shaoes are included (a-f) as well as one bacterial chain.. ...