OBJECTIVES: We sought to determine the association between changes in unemployment, healthcare spending and stomach cancer mortality. METHODS: Multivariate regression analysis was used to assess how changes in unemployment and public-sector expenditure on healthcare (PSEH) varied with stomach cancer mortality in 25 member states of the European Union from 1981 to 2009. Country-specific differences in healthcare infrastructure and demographics were controlled for 1- to 5-year time-lag analyses and robustness checks were carried out. RESULTS: A 1% increase in unemployment was associated with a significant increase in stomach cancer mortality in both men and women [men: coefficient (R)=0.1080, 95% confidence interval (CI)=0.0470-0.1690, P=0.0006; women: R=0.0488, 95% CI=0.0168-0.0809, P=0.0029]. A 1% increase in PSEH was associated with a significant decrease in stomach cancer mortality (men: R=-0.0009, 95% CI=-0.0013 to -0.005, P|0.0001; women: R=-0.0004, 95% CI=-0.0007 to -0.0001, P=0.0054). The

BioAssay record AID 95314 submitted by ChEMBL: Compound was tested in vitro for cytotoxic activity against KATO III human stomach cancer cell line by MTT assay.

TY - JOUR. T1 - Differential response to gamma radiation of human stomach cancer cells in vitro. AU - Jenkins, V. K.. AU - Barranco, S. C.. AU - Townsend, Courtney. AU - Perry, R. R.. AU - Ives, K. L.. PY - 1986. Y1 - 1986. N2 - In vitro effects of radiation were studied in two permanent cell lines (AGS and SII) from two patients with adenocarcinoma of the stomach and three permanent sublines from each cell line. Radiation survival parameters for AGS and SII parent cell lines and sublines were determined after in vitro irradiation of their cells with 0·5 to 10 Gy of 60Co gamma rays. The AGS and SII cell lines had different growth properties, DNA contents and radiation survival curves. Surviving fractions of SII parent cells (76 chromosomes) after 2·0 and 10 Gy were 1·22 and 17·8 times greater, respectively, than values for AGS parent cells (47 chromosomes). Sensitivities (D0) were 1·08 and 1·45 Gy for AGS and SII parent lines, respectively. The D0 values for AGS parent cells and sublines ...

Human Stomach Cancer Stem Cell (Plated cells are also available). 120 Population doublings or up to 12 passages. One million viable cells upon thawing of frozen cells, frozen vial of cells shipped in dry-ice. Cell Cultures from single donors, 1000 different cell cultures available, please indicate which lots you require from the 1000 donors. Source: Human Stomach Cancer tissue. Positive Markers: CD44, CD 133, SSEA3/4, Oct4, Tumorigenicity (,1000 cells), Alkaline Phosphatase, Aldehyde Dehydrogenase, Telomerase For non-academic use, please inquire for pricing. Cells are only guaranteed with purchase of Creative Bioarray Media and Creative Bioarray Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment ...

Introduction: Over the past decade potatoes with purple- and red-fleshed color rich in antioxidants (phenolic compounds - phenolic acids, anthocyanins etc.) have attracted the attention of consumers and scientists due to their appearance, flavour and biological properties. It is assumed that they have the potential to be an important source of biologically active compounds in the human nutrition and beneficial for human health. The aim of this study was to investigate whether potato tubers with coloured flesh might suppress human gastric adenocarcinoma cells (AGS cells) proliferation and viability. Object and methods: The field experiment was conducted in 2017 and 2018 on farm in Širvintos district (Lithuania). The study was implemented with 5 potato cultivars (Solanum tuberosum L) with different coloured flesh. In the conventional farming system the mix of universal complex fertilizers (Blaukorn Novatec N14P7K17) was used during potato crops vegetative period by inserting the 112 kg ha-1 ...

TY - JOUR. T1 - Abnormal expression and function of the E-cadherin-catenin complex in gastric carcinoma cell lines. AU - Jawhari, A. U.. AU - Noda, M.. AU - Farthing, M. J.G.. AU - Pignatelli, M.. PY - 1999/1/1. Y1 - 1999/1/1. N2 - Dysfunction of the cadherin-catenin complex, a key component of adherens junctions, is thought to confer invasive potential to cells. The aim of this study is to examine the expression and function of the E-cadherin/catenin complex in gastric carcinoma cell lines. Expression of E-cadherin, α, β and γ-catenin and p120(ctn), and of the adenomatous polyposis coil protein (APC), together with function of the cadherin-catenin complex was examined in a panel of gastric carcinoma cell lines, using immunocytochemistry, Western blotting and a cell-cell aggregation assay. Protein interactions were examined by sequential immunoprecipitation and immunoblotting with antibodies to E-cadherin, α, β and γ-catenin, p120(ctn) and APC. Abnormalities of E-cadherin, α- and ...

Saitama Cancer Center Research Institute. It is now well accepted that (-)-epigallocatechin gallate (EGCG) inhibits carcinogenesis in the digestive tract in rodents. To understand the mechanisms of anticarcinogenesis, we first studied growth inhibition by EGCG in human stomach cancer cell lines established at Seoul National University (SNU cell lines). Inhibition by EGCG of [3H]thymidine incorporation into eight SNU cell lines was examined, in relation to transforming growth factor-beta (TGF-beta) responsiveness. Various tea polyphenols derived from green tea and black tea induced growth inhibition and apoptosis of human stomach cancer cell line KATO III, and inhibition of tumor necrosis factor-alpha (TNF-alpha) release from the cells, in the order of (-)-epicatechin gallate (ECG), EGCG, (-)-epigallocatechin (EGC), teaflavins (TF) and (-)-epicatechin (EC). In addition, we demonstrated that EGCG inhibited TNF-alpha gene expression in KATO III cells, as well as okadaic acid-induced AP-1 and ...

TS-1 is an oral anticancer drug approved in Japan consisting of tegafur (a pro-drug of fluorouracil, 5-FU), gimeracil and oteracil potassium. The response rate of TS-1 in the untreated advanced gastric cancer patients was 44.6% in the late phase II study. In 2007, efficacy of the adjuvant therapy using TS-1 in the resected gastric cancer patients was demonstrated by ACTS-GC study group conducted in Japan. PSK is an oral anticancer drug approved in Japan consisting of protein-bound polysaccharide extracted from mycelium of Trametes (Coriolus) versicolor, a kind of mushroom. Even though survival benefit by PSK in combination with adjuvant chemotherapy using 5-FU or tegafur in the postoperative gastric cancer patients was already demonstrated, it is not uncertain about efficacy of combination therapy with PSK and TS-1 in gastric cancer. In this study, we compare efficacy and safety of postoperative adjuvant therapy using TS-1 or TS-1+PSK in the stage II or III gastric cancer patients ...

Long-term cigarette smoking increases the risk of gastric cancer mortality. Nicotine and NNK, tobacco-specific mitogens, were reported to correlate with cancer progression on gastric cancer. Since metastasis is the major cause of cancer death, the influence of nicotine on the migration of gastric cancer cells remains to be determined. In addition, how to improve the therapeutic efficacy is the important issue for gastric cancer. Our preliminary results indicated that nicotine or NNK treatment can enhance the migratory ability on gastric cancer. The enhancement effect was mediated through nicotinic acetylcholine receptor (nAChR). Silence of alpha7-nAChR expression may inhibit the enhanced effect by nicotine or NNK treatment, indicating that nAChR may be the target for preventing gastric cancer migration. Further, we also found that down-regulation of nAChR increased the sensitivity to chemo-therapy. Those result suggested that nAChR may be the novel therapeutic target for gastric cancer therapy. ...

NMDA receptor Type 2B (NMDAR2B) is a candidate TSG first identified in esophageal squamous cell carcinoma (ESCC). To evaluate NMDAR2B methylation in gastric cancer progression, we performed quantitative methylation-specific PCR (MSP), RTPCR and immnunohistochemistry (IHC) in primary gastric tissues and colony formation assays in gastric cancer cell lines. We found that the expression of NMDAR2B was reactivated by the demethylating agent, 5-aza-20-deoxycytidine, with or without trichostatin A in gastric cancer cell lines. Moreover, inactivation of NMDAR2B was found to be closely correlated with promoter methylation status in gastric cell lines and primary gastric tumors. IHC data also showed that NMDAR2B was specifically expressed in gastric epithelial cells and its expression was diminished or absent in gastric cancer epithelium. Quantitative analysis of NMDAR2B promoter methylation showed 61% (17/28) hypermethylation in primary gastric tumors versus 5% (1/20) in normal gastric tissues from ...

TY - JOUR. T1 - Intravenous paclitaxel against metastasis of human gastric tumors of diffuse type. AU - Tuan, Tsung Fan. AU - Tsai, Meng Li. AU - Yeh, Kai Chia. AU - Huang, Hsin Chieh. AU - Chung, Cheng Ta. AU - Huang, Chen Lung. AU - Han, Chia Hung. AU - Chen, Ching Ping. AU - Wang, Min Hsien. AU - Shen, Chien Chang. AU - Lai, Yiu Kay. AU - Lee, Wen-Sen. AU - Hwang, Ling-Ling. AU - Chen, Chiung Tong. PY - 2010/9. Y1 - 2010/9. N2 - Purpose: Gastric cancer is one of the leading cancerous diseases worldwide. It is diagnosed often at the advanced stage for which chemotherapy is the main treatment option. The prognosis remains poor for metastatic, especially the diffuse type, gastric cancers. We investigated the efficacy of intravenously administered paclitaxel treating metastases of locally disseminated gastric tumors of diffuse type. Methods: Transfection of green fluorescent proteins (GFP)-expressing plasmid into human gastric cancer MKN45 cells of diffuse type was performed, and MKN45-GFP cells ...

Angiogenesis is essential for tumor growth and metastasis and depends on the production of angiogenic factors by host and/or tumor cells. The role of angiogenesis and angiogenic factor expression in intestinal- and diffuse-type gastric cancer are undefined. Archival specimens of 51 intestinal-type and 38 diffuse-type human gastric carcinomas were examined for tumor vessel counts, angiogenic factor expression, and the presence or absence of angiogenic factor receptors on tumor endothelium using antibodies against vascular endothelial growth factor (VEGF) and its receptors (KDR and flt-1), basic fibroblast growth factor (bFGF) and its receptors (bek and flg), and factor VIII (endothelial cells). Vessel count and VEGF and bFGF expression were higher in intestinal-type than in diffuse-type gastric cancers (P = 0.01, P , 0.001, and P , 0.001, respectively). Similarly, vessel count and VEGF expression were higher in patients with liver metastasis than in patients with peritoneal dissemination (P = ...

Diffuse-type gastric carcinoma (DGC) cell lines with robust overexpression of Met are sensitive to Met inhibitors. (a) DGC and other gastric carcinoma cell line

TY - JOUR. T1 - Depth of response is a significant predictor for long-term outcome in advanced gastric cancer patients treated with trastuzumab. AU - Lee, Choong kun. AU - Kim, Seung seob. AU - Park, Saemi. AU - Kim, Chan. AU - Heo, Su Jin. AU - Lim, Joon Seok. AU - Kim, Hyunki. AU - Kim, Hyo Song. AU - Rha, Sun Young. AU - Chung, Hyun Cheol. AU - Park, Sohee. AU - Jung, Minkyu. PY - 2017/1/1. Y1 - 2017/1/1. N2 - Purpose: We aimed to determine and compare the predictive values of depth of response (DpR) and early tumor shrinkage (ETS) on long-term outcomes in gastric cancer patients treated with trastuzumab. Results: From a total of 368 computed tomography examinations, DpR and ETS were evaluated. DpR was a significant tumor-size metric in predicting PFS and OS, and showed better discriminatory ability (higher Ct indices, 0.6957 for PFS; 0.7191 for OS) than ETS. DpR ≥ 45% (vs. , 45%) was the optimal cutoffvalue, as it was best able to identify patients with longer PFS (median 9.0 vs. 6.3 ...

article{28d6110c-5d57-42d8-9243-f7ea1c6d6398, abstract = {This analysis of DNA-ploidy heterogeneity in advanced gastric carcinomas is consistent with the hypothesis of the emergence of a single aneuploid cell clone as a crucial mechanism in the progression from early gastric carcinoma to advanced gastric cancer. The prognostic value of DNA-ploidy in gastric cancers has been a matter of controversy. Tumour DNA-ploidy heterogeneity, the presence within the same tumour of multiple stemlines differing in DNA content, has been described in various tumours including gastric cancers. The occurrence of such heterogeneity has been accepted as an explanation for the divergent DNA-ploidy results in this type of tumours. A previous study of early gastric cancers suggested that in pure diploid superficial carcinomas, genetic instability might lead to a cell clone which has undergone a ploidy shift and is more aggressive. If so, this would initially result in DNA-ploidy heterogeneity. Proliferative dominance ...

This study is a single arm, single center phase II study of AZD5363 in combination with paclitaxel in patients with advanced gastric adenocarcinoma harboring

Aim: To assess whether secular trends in stomach cancer mortality were correlated with trends in infant mortality rate (IMR) or gross domestic product (GDP). Methods: Data from seven European countries were analyzed. We used Poisson regression to describe mortality trends among birth cohorts of 1865-1939 and correlation coefficients to determine associations with IMR/GDP. Results: Large differences were observed between birth cohorts in mortality from stomach cancer. In each country, these cohort differences were closely related to IMR/GDP levels at birth time. However, stronger associations were observed with measures of living conditions during later life. In comparisons between countries, stomach cancer mortality rates were not consistently related to national levels of IMR/GDP. Conclusion: General living conditions in childhood dont seem to have had a predominant effect on secular trends in stomach cancer mortality. The mortality decline is likely to be related to more specific factors, ...

Principal Investigator：YAMAGUCHI Koji, Project Period (FY)：2000 - 2001, Research Category：Grant-in-Aid for Scientific Research (C), Section：一般, Research Field：Digestive surgery

PAX8 was not only a mitotic factor, but identified as a transcription factor involved in the prognosis of human tumor patients. Elucidating the function of PAX8 on the pathology of stomach cancer was meaningful. PAX8 was found to be upregulated in primary stomach cancer tissue and the TCGA stomach cancer dataset. Interestingly, SOX13 and PAX8 showed consistent expression patterns, and the combined high PAX8 and SOX18 expression induced a worse prognosis of stomach cancer patients. SOX13 was further identified as a transcription factor of PAX8, and further affect Aurora B and Cyclin B1 expression, two cell cycle related factors of the downstream of PAX8, including. Furthermore, PAX8 depletion inducted G1-phase arrest and the decrease of EdU incorporation, cell viability and colony formation can be rescued by SOX13 overexpression. SOX13 participated in the elevated expression of PAX8, which promote the proliferation of stomach cancer cells. Therefore, SOX13 mediated PAX8 expression was recognized as a

PAX8 was not only a mitotic factor, but identified as a transcription factor involved in the prognosis of human tumor patients. Elucidating the function of PAX8 on the pathology of stomach cancer was meaningful. PAX8 was found to be upregulated in primary stomach cancer tissue and the TCGA stomach cancer dataset. Interestingly, SOX13 and PAX8 showed consistent expression patterns, and the combined high PAX8 and SOX18 expression induced a worse prognosis of stomach cancer patients. SOX13 was further identified as a transcription factor of PAX8, and further affect Aurora B and Cyclin B1 expression, two cell cycle related factors of the downstream of PAX8, including. Furthermore, PAX8 depletion inducted G1-phase arrest and the decrease of EdU incorporation, cell viability and colony formation can be rescued by SOX13 overexpression. SOX13 participated in the elevated expression of PAX8, which promote the proliferation of stomach cancer cells. Therefore, SOX13 mediated PAX8 expression was recognized as a

In this study, we determined the role of COX-2 inhibition in the prevention of sodium chloride enhanced gastric carcinogenesis induced by MNNG in Wistar rats. MNNG induced gastric cancer is a well established animal model of stomach carcinogenesis.16 The mutagen, when given in drinking water, induces intestinal metaplasia and adenocarcinoma in the pyloric mucosa of Wistar rats.1620 The histology of this induced gastric malignancy resembles the differentiated type of stomach cancer in humans. To enhance the carcinogenic effects of MNNG, highly concentrated sodium chloride solution was given to these animals in the initial six weeks.17 In the present study, 75% of MNNG treated animals developed gastric cancer at the end of 48 weeks, confirming that this is a highly successful model of gastric tumorigenesis.. Although the exact mechanism underlying MNNG induced gastric cancer remains poorly understood, previous studies showed that the genetic makeup of the animals may play a role.21 For example, ...

Numerous studies showed that drug resistance of gastric cancer cells could be modulated by the abnormal expression of microRNAs (miRNAs) which target multiple cell signaling pathways. The possible function of miR-1271 in the formation of cisplatin resistance in gastric cancer cells has been investigated in this study. miR-1271 was significantly down-regulated in gastric cancer tissues and various gastric cancer cell lines. Moreover, it was down-regulated in the cisplatin-resistant gastric cancer cell line SGC7901/cisplatin (DDP) and the down-regulation of miR-1271 in SGC7901/DPP cells was accompanied by the up-regulation of insulin-like growth factor 1 receptor (IGF1R)/insulin receptor substrate 1 (IRS1) pathway-related proteins, i.e., IGF1R, IRS1, serine/threonine-protein kinase mTOR (mTOR), and the apoptosis regulator Bcl-2 (BCL2), compared with the parental SGC7901 cells. Over-expression of miR-1271 sensitized SGC7901/DDP cells to cisplatin. Changes in the luciferase activity of reporter ...

Occurrence of metastatic cancer to the stomach is rare, particularly in patients with prostate cancer. Gastric metastasis generally presents as a solitary and submucosal lesion with a central depression. We describe a case of gastric metastasis arising from prostate cancer, which is almost indistinguishable from the undifferentiated-type gastric cancer. A definitive diagnosis was not made until endoscopic resection. On performing both conventional and magnifying endoscopies, the lesion appeared to be slightly depressed and discolored area and it could not be distinguished from undifferentiated early gastric cancer. Biopsy from the lesion was negative for immunohistochemical staining of prostate-specific antigen, a sensitive and specific marker for prostate cancer. Thus, false initial diagnosis of an early primary gastric cancer was made and endoscopic submucosal dissection was performed. Pathological findings from the resected specimen aroused suspicion of a metastatic lesion. Consequently,

OBJECTIVE: This study explored the effect of miR-26a-5p on cell proliferation, migration, and invasion in gastric cancer by targeting COL10A1. MATERIALS

Intro; Preface; Contents; Introduction; References; Contributors; Part I: Gastric Tumorigenesis; 1: Gastric Tumorigenesis: Role of Inflammation and Helicobacter pylori; 2: Genetic and Epigenetic Mechanisms in Gastric Cancer; Part II: Clinical and Pathological Characteristics; 3: Diagnosis and Surveillance: Endoscopic Hallmarks; 4: Pathological Diagnosis and Classification of Gastric Epithelial Tumours; 5: Diagnostic, Prognostic, Predictive and Therapeutic Tissue Biomarkers in Gastric Cancer; 6: Serum Biomarkers in Gastric Cancer; Part III: Gastric Cancer Therapy: Multimodal Treatment Approach 7: New Agents in the Treatment of Advanced Gastric Cancer: Targeted Therapy and Immunotherapy8: Combined Modality Treatment for Locally Advanced Gastric Cancer: Current Evidences and New Perspectives; 9: Surgical Strategies in Gastric Cancer; Part IV: Evolving Treatment Landscape in Gastric Cancer; 10: From Molecular Classification to Targeted Therapy for Gastric Cancer in the Precision Medicine Era; Part ...

A mouse model of metastasis of human gastric cancer is one of the most important tools for studying the biological mechanisms underlying human gastric cancer metastasis. In this paper, we established a mouse model of metastatic human gastric cancer in nude mice that has a higher rate of tumor formation and metastasis than existing models. To generate the mouse model of metastatic human gastric cancer, fresh tumor tissues from patients that have undergone surgery for gastric cancer were subcutaneously implanted in the right and left groins of nude mice. When the implanted tissue grew to 1 cubic centimeter, the mice were killed, and the tumor tissues were examined and resected. The tumor tissues were implanted into nude mice and subjected to pathological examination, immunohistochemical staining, and real-time PCR for cytokeratin 8/18 (CK8/18), E-cadherin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). The mice were also analyzed for metastasis in their

To examine toxicity and outcomes for patients treated with preoperative chemoradiotherapy (CRT) for gastric cancer. Patients with gastroesophageal (GE) junction (Siewert type II and III) or gastric adenocarcinoma who underwent neoadjuvant CRT followed by planned surgical resection at Duke University between 1987 and 2009 were reviewed. Overall survival (OS), local control (LC) and disease-free survival (DFS) were estimated using the Kaplan-Meier method. Toxicity was graded according to the Common Toxicity Criteria for Adverse Events version 4.0. Forty-eight patients were included. Most (73%) had proximal (GE junction, cardia and fundus) tumors. Median radiation therapy dose was 45 Gy. All patients received concurrent chemotherapy. Thirty-six patients (75%) underwent surgery. Pathologic complete response and R0 resection rates were 19% and 86%, respectively. Thirty-day surgical mortality was 6%. At 42 months median follow-up, 3-year actuarial OS was 40%. For patients undergoing surgery, 3-year OS, LC and

TSP50 (testes-specific protease 50) has been reported to be a candidate oncogene and is overexpressed in various cancers. Our previous study demonstrated that TSP50 protein is elevated in gastric cancer, and its high expression is associated with unfavorable prognosis and lymph node metastasis. However, the role of TSP50 in gastric cancer remains elusive. qRT-PCR, western blot were used to determine TSP50 expression in gastric cancer cell lines. Role of TSP50 in proliferation and invasion was examined by BrdU incorporation assay, cell count, wound healing and transwell assay. Immunohistochemistry and western blot were performed to identify the potential mechanisms involved. TSP50 was highly expressed in most of the gastric cancer cell lines at both mRNA and protein levels. Up-regulation of TSP50 in gastric cancer cells enhanced proliferation and invasiveness, whereas down-regulation of TSP50 by its specific shRNA decreased it. A negative correlation between TSP50 and E-Cadherin was found in gastric

In the present study, we examined the expression of Cyr-61 and COX-2 in 82 gastric cancer specimens and 43 non-tumor gastric mucosa specimens, and the results demonstrated the clinical significance of the expressions of Cyr-61 and COX-2 in determining the clinicopathologic features of gastric cancer and predicting its prognostic value.. Our results showed that Cyr-61 expression in gastric cancer tissues was significantly higher than that in the non-tumor gastric mucosa tissues. Furthermore, gastric cancer with higher Cyr-61 expression was associated with stronger invasion and metastasis abilities, advanced TNM stage and shorter survival time, suggesting that Cyr-61 plays an important role in the progression of gastric cancer. These results were consistent with the findings of Lin et al. [15]. They found Cyr-61 expression level was closely related to stages of gastric cancers, lymph node metastasis, and histological type. The 5-year survival time of patients with high Cyr-61 expression was ...

The expression of metallothionein (MT)-3 is often markedly reduced in gastric carcinoma (GC). The molecular mechanism of this MT-3 downregulation is unknown. Transcriptional silencing of MT-3 by methylation of CpG island was investigated by nucleotide sequencing and denaturing high performance liquid chromatography (DHPLC) analyses. We found that normal brain tissue and a xenografted GC that expressed MT-3 mRNA had unmethylated regions of the CpG island in intron1 of this gene. On the other hand, gastric cancer cell lines AGS and MKN445, a xenografted GC, and a representative primary gastric cancer that had no expression of MT-3 mRNA demonstrated hypermethylation of the MT-3 intron1 CpG island. Treatment of the gastric cancer cell lines with 5-azacytidine resulted in new expression of MT-3 mRNA in these cells. A quantifying DHPLC assay was developed to determine the methylation status of this specific region of the MT-3 gene.. Fifty-eight primary GC and their corresponding normal gastric ...

Gastric cancer is a heterogeneous disease histologically and genetically. Histologically, it is subdivided into intestinal and diffuse types by Lauren classification (4), but some gastric cancers cannot be easily classified in this way because these two types are frequently admixed within single tumors. Moreover, it is controversial as to whether Laurens classification is an independent prognostic factor. Genetically, many earlier studies have reported genetic alterations during gastric carcinogenesis and progression, e.g., p53 mutations (29), microsatellite instability (30), EBV infection (31), CpG island methylation (32), and chromosomal instability (33). Thus, gastric cancer is associated with various genetic alterations and no single genetic marker can predict gastric cancer biology or prognosis. The potential use of combinations of biomarkers instead of a single marker or histologic feature has been previously commented upon (19, 22, 34). Moreover, high-throughput analysis based on ...

Gastric cancer is the second leading cause of death from malignant disease worldwide and most frequently discovered in advanced stages. Because curative surgery is regarded as the only option for cure, early detection of resectable gastric cancer is extremely important for good patient outcomes. Therefore, noninvasive diagnostic modalities such as evolutionary endoscopy and positron emission tomography are utilized as screening tools for gastric cancer. To date, early gastric cancer is being treated using minimally invasive methods such as endoscopic treatment and laparoscopic surgery, while in advanced cancer it is necessary to consider multimodality treatment including chemotherapy, radiotherapy, and surgery. Because of the results of large clinical trials, surgery with extended lymphadenectomy could not be recommended as a standard therapy for advanced gastric cancer. Recent clinical trials had shown survival benefits of adjuvant chemotherapy after curative resection compared with surgery alone. In

Stomach tumors can have a genetic basis and hereditary diffuse gastric cancer (HDGC) represents approximately 1-3% of all gastric tumors.

Hereditary diffuse gastric cancer (HDGC) is a type of stomach (gastric) cancer that runs through families. HDGC is different from other types of stomach cancer because the cancer cells do not form into a solid tumor. Instead, HDGC cancer cells disperse or scatter throughout the stomach tissue. Because of this, this type of cancer spreads (metastasizes) more easily to other parts of the body.. Symptoms of this condition are often not recognized until the cancer is in later stages. These symptoms may include stomach pain, vomiting, and weight loss. Unfortunately, this type of cancer is difficult to catch in early stages. Therefore, if you have a history of HDGC in your family, it is important to get regular and frequent stomach (endoscopy) exams to detect for any cancer cell formation.. HDGC is caused by a genetic mutation in the CDH1 gene which is located on chromosome 16. When a mutation is present in the gene, the gene is unable to pass along the correct instructions to the rest of the cells of ...

Early gastric carcinoma (GC) is considered to be a curable cancer, as it progresses to the advanced stage following varying durations. Understanding the early stage of GC may provide an insight into its pathogenesis and contribute to reducing the mortality rate of this disease. To investigate the genomic aberrations associated with 22 cases of early GC, high-density microarray comparative genomic hybridization was performed in the present study. The most notable finding was copy number gains (log2 ratio >0.25) on the long arm of chromosome 8, which occurred in 77.3% (17/22) of GC cases, and the delineated minimal common region was 8q22.1-q24.3. More specifically, two amplified (log2 ratio >1) loci in the 8q22.1-q24.3 region were detected in 18.2% (4/22) of GC cases. The first loci covered a region of 102.4-107.9 kb, mapping on 8q22.3-q23.1, and comprised the transcription factor CP2-like 3 gene. The second loci, spanning 128.7-145.7 kb on 8q24.21-q24.3, comprised the representative oncogene of ...

Another factor associated with gastric cancer in certain populations is a high frequency of infection with H. pylori. Although it is not fully understood how H. pylori might increase the risk of gastric cancer, this rod-shaped helical bacterium has been classified since 1994 as a type I carcinogen by both the International Agency for Research on Cancer and the World Health Organization.11,14 Notably, H. pylori infection is most often associated with gastric cancers of the intestinal histologic subtype, which are observed more frequently in some areas of the world than others. Additionally, infection with H. pylori is not associated with all types of gastric cancer. H. pylori infection is only an independent risk factor for distal gastric cancer, and is not an independent risk factor for proximal forms of gastric cancer.5 Finally, although H. pylori infection is associated with 3- to 6-fold increase in the risk of developing gastric cancer, the vast majority of individuals infected with H. ...

TY - JOUR. T1 - Decreased Expression of MicroRNA-143 and-145 in Human Gastric Cancers. AU - Takagi, Takeshi. AU - Iio, Akio. AU - Nakagawa, Yoshihito. AU - Naoe, Tomoki. AU - Tanigawa, Nobuhiko. AU - Akao, Yukihiro. PY - 2009/7/1. Y1 - 2009/7/1. N2 - Objective: Downregulation of specific microRNAs (miRNAs) occurs in human tumors, which suggests a function for miRNAs in tumor suppression. We investigated the role of the miRNAs miR-143 and miR-145 in gastric cancers. Methods: The expression levels of miR-143 and miR-145 in the samples from 43 patients with gastric cancer were determined by real-time PCR using TaqMan assay. The growth inhibitory effect was estimated by the transfection of human gastric cancer cells with the miRNA. Results: The expression levels of miR-143 and-145 were decreased in most human gastric cancers examined, as previously reported to occur in colon tumors. The transfection of human gastric MKN-1 cells with miR-145 resulted in a greater growth inhibitory effect than that ...

MED30 is an essential member of the mediator complex that forms a hub between transcriptional activators and RNA polymerase II. However, the expressions and roles of MED30 have been poorly characterized in cancer. In this study, we examined the functional roles of MED30 during gastric cancer progression. It was found that MED30 was overexpressed in gastric cancer tissues and cell lines. Moreover, MED30 overexpression increased the proliferation, migration, and invasion of gastric cancer cells, whereas MED30 knockdown inhibited these effects. Furthermore the knockdown significantly inhibited tumorigenicity in SCID mice. MED30 also promoted the expressions of genes related to epithelial-mesenchymal transition and induced a fibroblast-like morphology. This study shows MED30 has pathophysiological roles in the proliferation, migration, and invasion of gastric cancer cells and suggests that MED30 should be viewed as a potent therapeutic target for malignant gastric carcinoma

1. McLean MH, El-Omar EM. Genetics of gastric cancer. Nature reviews Gastroenterology & hepatology. 2014;11:664-74 2. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinicians. 2015;65:87-108 3. Hu Y, Huang C, Sun Y, Su X, Cao H, Hu J. et al. Morbidity and Mortality of Laparoscopic Versus Open D2 Distal Gastrectomy for Advanced Gastric Cancer: A Randomized Controlled Trial. J Clin Oncol. 2016 4. Liu H, Li F, Zhu Y, Li T, Huang H, Lin T. et al. Whole-exome sequencing to identify somatic mutations in peritoneal metastatic gastric adenocarcinoma: A preliminary study. Oncotarget. 2016;7:43894-906 5. Zhang J, Huang JY, Chen YN, Yuan F, Zhang H, Yan FH. et al. Whole genome and transcriptome sequencing of matched primary and peritoneal metastatic gastric carcinoma. Scientific reports. 2015;5:13750 6. Huang B, Sun Z, Wang Z, Lu C, Xing C, Zhao B. et al. Factors associated with peritoneal metastasis in non-serosa-invasive ...

The calcium-dependent homophilic cell adhesion molecule and candidate suppressor gene, E (epithelial)-cadherin, plays a major role in the organization and integrity of most epithelial tissues. Diffusely growing gastric carcinomas show markedly reduced homophilic cell-to-cell interactions. We speculated that mutations in the E-cadherin gene may be responsible for the scattered phenotype of this type of carcinoma. For that reason we have examined E-cadherin in 26 diffuse type, 20 intestinal type and 7 mixed gastric carcinomas (Lauréns classification) at the DNA, RNA, and protein levels.. Reverse transcription polymerase chain reaction and direct sequencing of amplified E-cadherin complementary DNA fragments revealed inframe skipping of either exon 8 or exon 9 in 10 patients with diffuse tumors and an exon 9 deletion in one patient with a mixed carcinoma; both exons encode putative calcium binding domains. These alterations were not seen in nontumorous gastric tissues. Splice site mutations ...

List of Tables and Figures Figure Global Gastric Cancer Drugs Market Size (Million USD) Status and Outlook (2013-2018) Table Global Gastric Cancer Drugs Revenue (Million USD) Comparison by Regions (2013-2018) Figure Global Gastric Cancer Drugs Market Share by Regions (2013-2018) Figure United States Gastric Cancer Drugs Market Size (Million USD) and Growth Rate by Regions (2013-2018) Figure Europe Gastric Cancer Drugs Market Size (Million USD) and Growth Rate by Regions (2013-2018) Figure China Gastric Cancer Drugs Market Size (Million USD) and Growth Rate by Regions (2013-2018) Figure Japan Gastric Cancer Drugs Market Size (Million USD) and Growth Rate by Regions (2013-2018) Figure Southeast Asia Gastric Cancer Drugs Market Size (Million USD) and Growth Rate by Regions (2013-2018) Figure India Gastric Cancer Drugs Market Size (Million USD) and Growth Rate by Regions (2013-2018) Table Global Gastric Cancer Drugs Revenue (Million USD) and Growth Rate (%) Comparison by Product (2013-2018) Figure ...

The immunohistochemical expression of tenascin was examined in the normal adult mucosa of the stomach, primary tumours and lymph node metastases of gastric cancer patients. In normal gastric tissue tenascin was expressed in the muscularis mucosae, muscularis propria and vessel walls, however it was not expressed in either the mucosal connective tissue or the stromal tissue in the submucosal layer. In gastric cancer, tenascin was expressed in 35 of 85 primary tumours, and in 8 of 25 metastases in lymph nodes. Tenascin was located in the fibrous stroma surrounding foci of cancer. The expression of tenascin in the primary tumour did not correlate with the depth of invasion, lymph node metastasis or prognosis. Tenascin appears during the process of either malignant transformation or tumour progression in gastric cancer, and the positive expression of tenascin may be useful as a stromal marker for the early detection of gastric cancer.

Background: Gastric adenocarcinoma is the fifth most common cancer and third leading cause of cancer mortality in the world. In the US, outcomes for gastric cancer are dismal with only 28% surviving to 5 years. Upper gastrointestinal endoscopy is the gold standard for early detection of gastric tumors and used in countries with high prevalence for mass screening. However, due to the low prevalence of gastric cancer in the general U.S. population, a national screening program is not cost effective. Tools to identify patients at higher risk of gastric cancer may help identify subpopulations that should be referred for opportunistic screening.. Purpose: To characterize the questionnaire development of a comprehensive gastric cancer risk assessment survey instrument that will be used to collect primary data in a large-scale case-control study. This primary data will be used to create a predictive model and determine items that best discriminate between gastric cases and controls, to ultimately ...

A human gastric adenocarcinoma cell line, HGT-1, was established in vitro from the primary tumor of a 60-year-old patient. Histological examination of the tumor revealed a poorly differentiated adenocarcinoma. Primary tumor cells were cloned in soft agarose and gave rise to tumor colonies. The procedures enabling us to form a continuous cell line from the agarose colonies are described. The cultured cells grew as monolayers of closely apposed polygonal cells with a population-doubling time of 19.48 ± 1.20 (S.E.) hr during exponential growth at passage 59. They had an epithelial morphology. Ultrastructural studies revealed the presence of microvilli and tight junctions. The HGT-1 cell line is tumorigenic in nude mice and has a hyperdiploid karyotype with a modal number of 57 chromosomes. It exhibits numerous marker chromosomes. These human gastric epithelial cells do not secrete mucus or carcinoembryonic antigen. They exhibit functional histamine H2-receptors mediating cellular cyclic adenosine ...

Dr Contente speaks with ecancer at the ESMO 2017 Congress saying that advanced gastric cancer is associated with productivity loss for both patients and care

TY - JOUR. T1 - A combination of cyclosporin-A (CsA) and interferon-gamma (INF-γ) iduces apoptosis in human gastric carcinoma cells. AU - Morisaki, T.. AU - Matsunaga, H.. AU - Beppu, K.. AU - Ihara, E.. AU - Hirano, K.. AU - Kanaide, H.. AU - Mori, M.. AU - Katano, M.. N1 - Copyright: Copyright 2007 Elsevier B.V., All rights reserved.. PY - 2000. Y1 - 2000. N2 - Modulation of interferon-γ effect by other drug may enhance its tumor specific activity. The apoptosis inducing effect of interferon-γ and its modulation by cyclosporin-A or tacrolimus (FK-506) were investigated in in vitro and ex vivo experiments. We found that a combination of cyclosporin-A (CsA) and recombinant interferon-gamma (rIFN-γ) induced significant apoptosis in all four types of human gastric carcinoma cells tested but not in normal cells such as human peripheral blood mononuclear cells (PBMCs), human omentura-derived mesothelial cells, or human umbilical vein endothelial cells (HUVECs) in vitro. Apoptosis was also ...

Background: Lactoferrin is a glycoprotein with a molecular weight of 80 kDa, as a member of the transferring family. In recent investigations the important biological properties such as anti microbial, antiviral and anticancer activity of lactoferrin were studied. In the present study, the effect of antitumor induced by lactoferin was evaluated on stomach ...

Yoshiyuki Kawakami, PhD, Hidenori Fujii, PhD, Yuki Hirose, PhD. Japanese Red Cross Fukui Hospital. Aims: We previously reported that laparoscopic gastrectomy for advanced gastric cancer could be feasible with some difficult situation such in cases with D2 lymph node dissection for metastasis. Thus we introduced laparoscopic surgery after chemotherapy for unresectable gastric cancer as conversion surgery. It is expected that our technique could be useful for treating these cases.. Methods: From April of 2010 to March of 2014, 79 consecutive patients with advanced gastric cancer diagnosed as cStage IIIA/IIIB/IV were indicated for chemotherapy regimen of Docetaxel/CDDP/TS1 (DCS), CDDP/TS1 (CS), Docetaxel/TS1 (DS). We conducted to study our technique of laparoscopic gastrectomy as conversion surgery for 2 patients expected for R0/R1 resection.. Results: Clinical records of 79 cases of gastrectomy for advanced gastric cancer (From April of 2010 to March of 2014) were analyzed retrospectively in ...

Yoshihiro Hiramatsu, MD, PhD1, Hirotoshi Kikuchi, MD, PhD1, Amane Hirotsu, MD1, Wataru Soneda, MD1, Tomohiro Murakami, MD1, Tomohiro Matsumoto, MD1, Yusuke Ozaki, MD1, Kinji Kamiya, MD, PhD1, Takanori Sakaguchi, MD, PhD1, Hiroyuki Konno, MD, PhD, FACS2, Hiroya Takeuchi, MD, PhD, FACS1. 1Department of Surgery, Hamamatsu University School of Medicine, 2Hamamatsu University School of Medicine. Background: Robot-assisted surgery using da Vinci Surgical System(DVSS) is thought to have many advantages over conventional laparoscopic surgery. It was reported that the use of the surgical robot might reduce surgery-related complications, then a multi-institutional historically controlled prospective cohort study on the feasibility, safety, effectiveness and economical efficiency of robotic gastrectomy (RG) for resectable gastric cancer was conducted in Japan. This study evaluated the safety of RG using DVSS Xi.. Methods: This single-center, prospective phase II study included patients with resectable ...

Connective tissue growth factor (CTGF) has been shown to be implicated in tumor development and progression. However, the role of CTGF in gastric cancer remains largely unknown. In this study, we showed that CTGF was highly expressed in gastric cancer tissues compared with matched normal gastric tissues. The CTGF expression in tumor tissue was associated with histologic grade, lymph node metastasis and peritoneal dissemination (P | 0.05). Patients with positive CTGF expression had significantly lower cumulative postoperative 5 year survival rate than those with negative CTGF expression (22.9% versus 48.1%, P | 0.001). We demonstrated that knockdown of CTGF expression significantly inhibited cell growth of gastric cancer cells and decreased cyclin D1 expression. Moreover, knockdown of CTGF expression also markedly reduced the migration and invasion of gastric cancer cells and decreased the expression of matrix metalloproteinase (MMP)-2 and MMP-9. Animal studies revealed that nude mice injected with the

BACKGROUND: In areas with high prevalence of Helicobacter pylori (Hp) infection and high gastric cancer mortality such as China and Japan, Hp status is not a good marker for increased risk of gastric cancer. The increased risk associated with CagA+ Hp strains are controversial in these areas as well. Hong Kong has a moderate to high prevalence of Hp infection and a gastric cancer mortality of 7.9/105 population. We examine the relationship between Hp, particularly CagA+ strains, and gastric cancer in Hong Kong adults. METHODS: Sera from 52 patients (29 male and 23 females, mean age 59.8 yrs) with non-cardiac type of gastric cancer and 52 age and sex matched asymptomatic controls were examined for anti-Hp antibody by using the commercial ELISA kit (Bio-rad, USA). CagA status was determined by an anti-CagA antibody assay using a purified recombinant CagA fusion protein as antigen using the method described(Am J Gastroenterol 1996:91:949). Results: Hp infection was found in 42 (80.8%) and 35 ...

Gastric cardia adenocarcinoma (GCA) is an aggressive subtype of gastric cancer with a high metastatic rate. However, the metastatic biomarker of GCA has not been established. To search for the biomarker for GCA metastasis, we here examined expression of the Hippo signaling effector WWTR1 (WW domain containing transcription regulator 1, commonly listed as TAZ) in tumor tissue samples from 214 GCA cases using the tissue microarray assay (TMA), and statistically analyzed association of the WWTR1 expression with metastasis-related pathological outcomes and cumulative survival of the GCA patients. Furthermore, shRNA knockdown was used to determine the role of WWTR1 in promoting cell migration in gastric cancer cells. The results have shown that WWTR1 is overexpressed in 66.4% of the GCA tumor samples. Expression of WWTR1 has a significant inverse correlation with cumulative survival of GCA patients (p | 0.01). WWTR1 positive patients had a mean survival of 56.9 ± 4.4 months, comparing to WWTR1 negative mean

TY - JOUR. T1 - Long-term Outcomes of Laparoscopic Versus Open Surgery for Clinical Stage II/III Gastric Cancer. T2 - A Multicenter Cohort Study in Japan (LOC-A Study). AU - Kinoshita, Takahiro. AU - Uyama, Ichiro. AU - Terashima, Masanori. AU - Noshiro, Hirokazu. AU - Nagai, Eishi. AU - Obama, Kazutaka. AU - Tamamori, Yutaka. AU - Nabae, Toshinaga. AU - Honda, Michitaka. AU - Abe, Takayuki. N1 - Funding Information: Disclosure: This study received Novartis Pharma Research Grants, Takeda Science Foundation, and Research Grants from Japanese Gastric Cancer Association. Publisher Copyright: © 2018 The Author(s). Published by Wolters Kluwer Health, Inc. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.. PY - 2019/5/1. Y1 - 2019/5/1. N2 - A large-scale multicenter historical cohort study was conducted to investigate the efficacy of laparoscopic gastrectomy (LG) in comparison to open gastrectomy (OG) for locally advanced gastric cancer.Background:LG is now practiced widely, but its ...

Linitis plastica, also known as Brintons disease or leather bottle stomach, is a morphological variant of diffuse (or infiltrating) stomach cancer.. Causes of linitis plastica could be lye ingestion or metastatic infiltration of the stomach, particularly breast and lung carcinoma.[1] It is not associated with H. pylori infection or chronic gastritis. The risk factors are undefined, except for rare inherited mutations in E-cadherin, which are found in about 50% of diffuse-type gastric carcinomas.[1]. ...

I am very sorry about your husbands diagnosis. I have no personal knowledge about gastric cancer and never heard of linitis plastica until I saw your post. I hope that someone more competent responds to you soon.. I am going to post some links below. Dont read them if youre easily upset by statistics / prognosis. Each person reacts to treatment differently and there is no expiration date on anyone.. From my own experience (I have a different type of cancer) it is always helpful to get a second or third opinion from a reputable oncologist in Israel or abroad.. Helicobacter pylori is a cause of gastric (stomach) cancer. http://www.cancer.gov/cancertopics/factsheet/Risk/h-pylori-cancer. However H. pylori does not cause Linitis plastica, also known as Brintons disease or leather bottle stomach, a morphological variant of diffuse (or infiltrating) stomach cancer.. Causes of linitis plastica could be lye ingestion or metastatic infiltration of the stomach, particularly breast and lung carcinoma. ...

TY - JOUR. T1 - Clinicopathological significance of N-cadherin and VEGF in advanced gastric cancer brain metastasis and the effects of metformin in preclinical models. AU - Jun, Kyong Hwa. AU - Lee, Jung Eun. AU - Kim, Se Hoon. AU - Jung, Ji Han. AU - Choi, Hyun Joo. AU - Kim, Young Il. AU - Chin, Hyung Min. AU - Yang, Seung Ho. PY - 2015/10/1. Y1 - 2015/10/1. N2 - Gastric cancer is the second most common cause of cancer-related death worldwide. Although brain metastasis is a rare complication of gastric cancer, no standard therapy for gastric cancer brain metastasis has been established. We attempted to identify biological markers that predict brain metastasis, and investigated how to modulate such markers. A case-control study of patients newly diagnosed with gastric cancer who had developed brain metastasis during follow-up, was conducted. These patients were compared with patients who had advanced gastric cancer but no evidence of brain metastasis. Immunohistochemistry was used to analyze ...

Operation is the only curative treatment for gastric cancer patients. However, the rate of recurrence is high up to 60%. The 5 years overall survival of patient at stage IIIb or more advanced stage is still poor and approximately 8-28%. Adjuvant chemotherapy is critical for improving efficacy further. Unfortunately, the optimal adjuvant regimen is not identified yet. The standard adjuvant treatments of American and European patients are not accepted widely in Asia area because of different operation procedure and patients tolerability. Results of two critical trials indicated that S-1 alone as Japanese standard adjuvant chemotherapy could not improve the survival of stage IIIb advanced stage gastric cancer patients while the Korean standard regimen XELOX could. This implied that the more intensive chemotherapy must be used for the patients with higher risk of relapse. The proportion of the stage IIIb-IIIc Chinese gastric cancer patients is much larger than that of Japan and Korean. However, no ...

The null genotype of GSTM1 have been implicated in gastric cancer risk, but numerous individual studies showed mixed, or even conflicting results. Thus, a meta-analysis was performed. We identified 54 individual studies involving 9,322 cases and 15,118 controls through computer-based searches of PubMed, Embase, and Cochrane Library. It was found that the null genotype of GSTM1 was associated with an increased gastric cancer risk (OR = 1.207, 95% CI: 1.106-1.317, P | 0.001), under the random-effects model (I2 : 49.9%, PQ |0.001). From stratification analyses for ethnicity, alcohol drinking, Helicobacter pylori infection, an effect modification of gastric cancer risk was found in the subgroups of ethnicity, smoking status, Helicobacter pylori infection, whereas null result was found in the subgroups of alcohol drinking. We also undertook gene-gene interaction analysis between GSTM1 and GSTT1 genes for gastric cancer risk, and the results indicated that the dual null genotypes of GSTM1 and GSTT1 might

The identification of circulating tumor cells (CTCs) may provide important prognostic information in several types of solid tumors, including gastric cancer. The aim of this study was to investigate whether CTC count may be used to predict survival in patients with advanced gastric cancer treated with chemotherapy. The CELLection™ Epithelial Enrich kit was used to isolate and purify CTCs from samples of peripheral blood. immunofluorescent staining was used for CTC counting. High CTC counts were associated with poor tumor differentiation and high serum CEA levels (P=0.021 and 0.005, respectively). After 3 months, 16 patients with decreasing CTC counts after the first cycle of chemotherapy obtained complete response, partial response or stable disease, while 13 patients with increasing CTC counts developed progressive disease. The patients with decreasing CTC counts also exhibited longer progression‑free survival (PFS) (P≤0.001) and overall survival (OS) (P=0.002) compared with those with ...

BACKGROUND:Chemotherapy and radiation therapy provide limited improvement in survival of gastric cancer patients after tumor resection. It is essential to develop a novel therapeutics for gastric cancer. In the recent years, cytokine-induced killer cells (CIKs)-based adoptive immune therapy has been explored in gastric cancer patients. Due to the small number of patients included in each clinical trial and low-power statistical analysis, the effectiveness of this approach is still unclear. To address this issue, we systemically analyzed the relevant clinical trial data published in recent years by powerful statistical meta-analysis. MATERIAL AND METHODS:Clinical data was searched by multiple electronic databases with a term

to other tissues, such as the liver or nearby bones.. Symptoms of diffuse gastric cancer occur late in the disease and can include stomach pain, nausea, vomiting, difficulty swallowing (dysphagia), decreased appetite, and weight loss. If the cancer metastasizes to other tissues, it may lead to an enlarged liver, yellowing of the eyes and skin (jaundice), an abnormal buildup of fluid in the abdominal cavity (ascites), firm lumps under the skin, or broken bones.. In HDGC, gastric cancer usually occurs in a persons late thirties or early forties, although it can develop anytime during adulthood. If diffuse gastric cancer is detected early, the survival rate is high; however, because this type of cancer is hidden underneath the stomach lining, it is usually not diagnosed until the cancer has become widely invasive. At that stage of the disease, the survival rate is approximately 20 percent.. Some people with HDGC have an increased risk of developing other types of cancer, such as a form of breast ...

Drug resistance is a major factor for the limited efficacy of chemotherapy in gastric cancer treatment. Hypoxia-inducible factor-1α (HIF-1α), a central transcriptional factor in hypoxia, is suggested to participate in the resistance. Here, we identified a hypoxia-mimic (cobalt chloride) sensitive gastric cell line BGC-823 to explore whether diosgenin, an aglycone of steroidal saponins, can inhibit cancer cell invasion and survival of solid tumor in a hypoxic mimic microenvironment. We have shown that diosgenin is a potent candidate for decreasing the ability of invasion and survival in cobalt chloride treated BGC-823 cells. In addition, when combined with HIF-1α specific short hairpin RNA (shRNA), diosgenin can inhibit BGC-823 cells more effectively. The anti-invasion role of diosgenin may be related to E-cadherin, integrinα5 and integrinβ6. These results suggest that diosgenin may be a useful compound in controlling gastric cancer cells in hypoxia condition, especially when combined with down

Diffuse gastric cancer (DGC) is one of the two main types of stomach cancer. Histologically, DGC can be subdivided into poorly differentiated carcinoma and signet ring cell carcinoma (SRCC). Carriers of germline mutations in the gene encoding E-cadherin (CDH1) are predisposed to DGC (hereditary DGC, HDGC). Essentially all asymptomatic mutation carriers present with occult, multifocal SRCC confined to the gastric mucosa before they develop advanced disease. Initial characterisation of these early HDGC foci (referred to as eHDGC) suggests an indolent nature of these lesions, contrary to their current pathological definition as malignant disease. The main aim of this study was to determine if genomic instability is an early event in HDGC and how it might lead to disease progression. We assessed chromosomal aberrations in the early intramucosal eHDGCs by using array comparative genomic hybridisation (CGH). Taking advantage of possibly the largest eHDGC collection worldwide, we analysed tissue from ...

Chronic Helicobacter pylori infection is recognized as a cause of gastric cancer. H. pylori adhesion to gastric cells is mediated by bacterial adhesins such as sialic acid-binding adhesin (SabA), which binds the carbohydrate structure sialyl-Lewis x. Sialyl-Lewis x expression in the gastric epithelium is induced during persistent H. pylori infection, suggesting that H. pylori modulates host cell glycosylation patterns for enhanced adhesion. Here, we evaluate changes in the glycosylation-related gene expression profile of a human gastric carcinoma cell line following H. pylori infection. We observed that H. pylori significantly altered expression of 168 of the 1,031 human genes tested by microarray, and the extent of these alterations was associated with the pathogenicity of the H. pylori strain. A highly pathogenic strain altered expression of several genes involved in glycan biosynthesis, in particular that encoding β3 GlcNAc T5 (β3GnT5), a GlcNAc transferase essential for the biosynthesis of ...

Gastric cancer is is almost unique among cancer bc almost 80% of all gastric cancers are caused by chronic inflammation as a result of infection with Helicobacter Pylori (H. Pylori). Infection with H. Pylori is relatively easily treated oral antibiotics and antacids. Clinical studies in China, where the risk of gastric cancer is high, demonstrated that treatment of H. Pylori infection can reduce cancer gastric risk. This begs the obvious question of why arent we screening and treating patients with chronic H. Pylori infections before they develop gastric cancers ...

This study shows that expression of CA-AhR in transgenic mice induces lethality beginning at 6 months of age. This effect correlated with the development of severe tumors in the stomach, demonstrating an oncogenic potential of the AhR. It has been difficult to interpret the histopathology of the stomach tumors in the CA-AhR mice unambiguously. The well organized glandular structures and low levels of cellular atypia argue for a benign phenotype. On the other hand, the reduced life span, the aggressive, expanding invasion of all stomach layers, and the adherence to surrounding organs point toward a more malignant phenotype. Intestinal metaplasia, which is regarded as a precancerous lesion (18), was widespread in the CA-AhR tumors. Furthermore, a subgroup of human intestinal-type gastric carcinoma contains highly differentiated cells (19). Given the striking gastric oncogenic phenotype of the CA-AhR mice it is interesting to note that several physiological candidates of receptor ligands are indole ...

TY - JOUR. T1 - S-1 plus leucovorin and oxaliplatin versus S-1 plus cisplatin as first-line therapy in patients with advanced gastric cancer (SOLAR). T2 - a randomised, open-label, phase 3 trial. AU - Kang, Yoon Koo. AU - Chin, Keisho. AU - Chung, Hyun Cheol. AU - Kadowaki, Shigenori. AU - Oh, Sang Cheul. AU - Nakayama, Norisuke. AU - Lee, Keun Wook. AU - Hara, Hiroki. AU - Chung, Ik Joo. AU - Tsuda, Masahiro. AU - Park, Se Hoon. AU - Hosaka, Hisashi. AU - Hironaka, Shuichi. AU - Miyata, Yoshinori. AU - Ryu, Min Hee. AU - Baba, Hideo. AU - Hyodo, Ichinosuke. AU - Bang, Yung Jue. AU - Boku, Narikazu. PY - 2020/8. Y1 - 2020/8. N2 - Background: S-1 plus leucovorin and oxaliplatin showed promising efficacy for treatment of advanced gastric cancer in a randomised phase 2 study. We aimed to evaluate the efficacy and safety of oral TAS-118 (S-1 plus leucovorin) and oxaliplatin versus S-1 plus cisplatin in patients with advanced gastric cancer. Methods: We did a randomised, open-label, phase 3 trial in ...

Gastric cancer surgery and gastrointestinal endoscopy are some of the gastrointestinal treatments offered by Dr. Gary Crosthwaite in Epworth, Melbourne.

TY - JOUR. T1 - Cutaneous metastasis from primary gastric cancer. T2 - A case report and review of the literature. AU - Cesaretti, Manuela. AU - Malerba, Michele. AU - Basso, Valeria. AU - Boccardo, Chiara. AU - Santoni, Roberta. AU - DAlessandro, Gabriele. AU - Weiss, Andrea. AU - Campisi, Corrado. AU - De Cian, Franco. PY - 2014. Y1 - 2014. N2 - Cutaneous metastases of internal neoplasms are uncommon. They can be metachronous or synchronous to the primary tumor and typically appear late in the course of advanced malignant disease. Gastric cancer rarely is reported as a cause of cutaneous metastasis; the most common metastatic sites are the liver, peritoneal cavity, and lymph nodes. We report a case of cutaneous metastasis from a primary gastric tumor that had been treated 6 years prior. There was no visceral invasion. The patient was treated successfully via a relaparotomy to exclude any macroscopic abdominal recurrence and complete excision of the lesion with a plastic flap to compensate for ...

In recent years, increasing research has focused on the relationship between cytokines and tumorigenesis. It has been suggested that cytokines may be a new therapeutic option for tumors (16-20). In our experiments, we demonstrated that IFN-λ1 inhibited the growth of gastric carcinoma cells in a concentration-dependent manner. These data suggest that IFN-λ1 may be a potential antitumor agent for the treatment of gastric cancer.. Impaired apoptotic induction and dysregulated cell cycle progression are important factors in cancer development. Accordingly, inhibition of cell cycle regulation is particularly useful in the treatment of cancer. In our in vitro study, we demonstrated the apoptosis-inducing effects of IFN-λ1 in gastric carcinoma cells using PI cell cycle analysis, Annexin V and PI staining as well as activated caspase-3. Our study showed that IFN-λ1 induced G1 phase arrest and apoptosis in the gastric carcinoma cells.. Experimental evidence suggests that apoptosis can be mediated by ...

The DNA damage repair gene PALB2 is well described as increasing risk of breast and ovarian cancer when mutated. In a sequencing study of hereditary diffuse gastric cancer (HDGC) families, Eleanor Fewings as a part of the Tischkowitz lab, has shown that predicted pathogenic mutations in PALB2 also occur in high risk HDGC families. Mutations in the E-cadherin gene CDH1 greatly impact HDGC risk, however many HDGC families are identified without mutations in this gene. For these families, the choice to undergo risk reducing surgery is based on poorly informed risk calculations. This study named new genetic candidates for HDGC risk including PALB2, ATR, NBN, and RECQL5. Identification of these genes could provide families with HDGC who dont carry CDH1 pathogenic variants with improved information about the risks associated with their disease and allow them to make informed decisions about risk reduction and disease management.. Link: ...

Long noncoding RNAs (lncRNAs) have emerged as important regulators in the development and progression of gastric cancer (GC). ARHGAP27P1 is a pseudogene-derived lncRNA, and it has been found to be associated with GC in our preliminary study, but this association has not been studied further. Herein, we confirmed that ARHGAP27P1 was significantly downregulated in GC tissues, plasma and cells. Low expression of ARHGAP27P1 was closely associated with advanced TNM stage, increased invasion depth and lymphatic metastasis. Low ARHGAP27P1 expression also predicted a poor prognosis in GC patients. Functionally, overexpression of ARHGAP27P1 inhibited proliferation, invasion, and migration in GC cells, while silencing of ARHGAP27P1 showed the opposite effects. Mechanistic investigations showed that ARHGAP27P1 had a key role in G0/G1 arrest. We further demonstrated that ARHGAP27P1 was associated with Jumonji-domain containing 3 (JMJD3) and that this association was required for the demethylation of H3K27me3,

BACKGROUND: The data are scarce on the outcome for elderly patients presenting with resectable gastric cancer in the West who have been treated with minimally invasive surgery. This report presents the authors early experience with totally laparoscopic gastric resections for cancer in elderly patients. METHODS: A total of 20 patients underwent laparoscopic gastrectomy procedures: 14 distal, 5 subtotal, and 1 total gastrectomy. The male-to-female ratio was 15 to 5. The ages ranged from 75 to 88 years (mean, 80 years). RESULTS: All cases were managed laparoscopically with R0 resection. Four patients needed high-dependency unit care postoperatively. There were no perioperative deaths. The median time required for the procedure was 212 min, and time to diet was 4 days. The hospital stay was 8 days. Four patients experienced significant complications, with two patients requiring reoperation. The pathology was adenocarcinoma for 17 patients and high-grade dysplasia for 3 patients. CONCLUSION: Among elderly

Ian Chau, MD, discusses interim safety and clinical activity results in patients with advanced gastric or gastroesophageal junction adenocarcinoma from a multi-cohort phase I study of ramucirumab plus pembrolizumab.

Gastric cancer exhibits familial clustering, and gastric cancer familial relatives (GCF) tend to present with corpus-predominant gastritis and precancerous lesions as SPEM or IM after H. pylori infection. The study determined whether the children of gastric cancer patients (GCA) had genomic single nucleotide polymorphisms (SNPs) predisposed to the gastric precancerous lesions as spasmolytic polypeptide-expressing metaplasia (SPEM) or intestinal metaplasia (IM). There were 389 family relatives of 193 non-cardiac GCA and 173 duodenal ulcer patients (DU), received blood sampling for DNA collection. The differences of the risk alleles of SNPs in the ITGA5, ITGB1, IL-10, COX-2, RUNX3, and TFF2 genes were compared between 195 children of GCA and 143 DU. The children of GCA had higher allele frequencies of ITGA5-1160 T-carrier (P = 0.006, OR[95% CI] = 2.2[1.2-4]), ITGB1-1949 A-carrier (P = 0.047; OR[95% CI] = 2.8[1.4-5.3]), ITGB1 + 31804 C-carrier (P = 0.013; OR[95% CI] = 4.7[1.7-13.0]), IL-10-592 AA (P = 0

O:13:\PanistOpenUrl\:36:{s:10:\\u0000*\u0000openUrl\;N;s:6:\\u0000*\u0000idc\;N;s:6:\\u0000*\u0000fmt\;s:7:\journal\;s:6:\\u0000*\u0000doi\;s:17:\10.1159\/000011956\;s:6:\\u0000*\u0000pii\;s:0:\\;s:7:\\u0000*\u0000pmid\;s:0:\\;s:9:\\u0000*\u0000atitle\;s:82:\N-acetyl-\u03b2-hexosaminidase activity and isoenzymes in human gastric adenocarcinoma\;s:9:\\u0000*\u0000jtitle\;s:8:\Oncology\;s:9:\\u0000*\u0000stitle\;s:8:\Oncology\;s:7:\\u0000*\u0000date\;s:4:\1999\;s:9:\\u0000*\u0000volume\;s:2:\56\;s:8:\\u0000*\u0000issue\;s:1:\2\;s:8:\\u0000*\u0000spage\;s:3:\142\;s:8:\\u0000*\u0000epage\;s:3:\154\;s:8:\\u0000*\u0000pages\;s:0:\\;s:7:\\u0000*\u0000issn\;s:9:\0030-2414\;s:8:\\u0000*\u0000eissn\;s:0:\\;s:9:\\u0000*\u0000aulast\;s:10:\GIL-MARTIN\;s:10:\\u0000*\u0000aufirst\;s:1:\E\;s:9:\\u0000*\u0000auinit\;s:0:\\;s:10:\\u0000*\u0000auinitm\;s:0:\\;s:5:\\u0000*\u0000au\;a:3:{i:0;s:24:\RODRIGUEZ-BERROCAL, F. ...

TY - JOUR. T1 - [A case report-highly advanced gastric cancer leading to perforation during neoadjuvant chemotherapy with docetaxel, cisplatin and S-1].. AU - Mihara, Koki. AU - Egawa, Tomohisa. AU - Kemmochi, Takeshi. AU - Irino, Tomoyuki. AU - Okamura, Akihiko. AU - Inaba, Yusaku. AU - Eto, Eiichi. AU - Segami, Kenki. AU - Ito, Yasuhiro. AU - Hayashi, Shinobu. AU - Nagashima, Atsushi. PY - 2011/11. Y1 - 2011/11. N2 - A 70-year-old man was found to have advanced gastric cancer with a deep ulcer and multiple lymph-node metastases. Although the tumor was resectable, we predicted that the patient would have a poor outcome. We therefore administered neoadjuvant chemotherapy with docetaxel, cisplatin, and S-1 to improve the prognosis before curative resection. On day 15 of chemotherapy, sudden abdominal pain occurred, and we performed an emergency surgery for a diagnosis of panperitonitis due to gastric cancer perforation. The defect in the gastric wall was about 2 cm in diameter and was located in ...

TY - JOUR. T1 - Histologic and immunohistochemical differences between hereditary and sporadic diffuse gastric carcinoma. AU - Lee, Hee Eun. AU - Smyrk, Thomas Christopher. AU - Zhang, Lizhi. PY - 2018/4/1. Y1 - 2018/4/1. N2 - We aimed to identify histopathologic features unique in hereditary diffuse gastric cancer (HDGC) by comparing with its sporadic counterpart (SDGC). 11 patients with confirmed CDH1 mutation who were found to have HDGC in a prophylactic total gastrectomy were collected. Median age of HDGC patients was 39 years (range 24-57). All HDGC cases had intramucosal signet ring cell carcinoma. Twenty-three invasive tumor foci from 7 patients with HDGC were available for ancillary studies, and we evaluated each focus separately. Almost all foci (20/23) showed two distinct tumor cell populations, namely, large signet ring cells and small signet ring cells. The large cells were located just beneath the surface epithelium and were positive for mucicarmine and pCEA, while the small cells ...

The E295K DNA polymerase beta gastric cancer-associated variant interferes with base excision repair and induces cellular transformation.

BACKGROUND: The contribution of genetic variation in DNA repair genes to gastric cancer (GC) risk remains essentially unknown. The aim of this study was to explore the relative contribution of DNA repair gene polymorphisms to GC risk and severe chronic atrophic gastritis (SCAG). Method A nested case control study within the EPIC cohort was performed including 246 gastric adenocarcinomas and 1175 matched controls. Controls with SCAG (n = 91), as defined by low pepsinogen A (PGA) levels, and controls with no SCAG (n = 1061) were also compared. Twelve polymorphisms at DNA repair genes (MSH2, MLH1, XRCC1, OGG1 and ERCC2) and TP53 gene were analysed. Antibodies against Helicobacter pylori were measured. RESULTS: No association was observed for any of these polymorphisms with stomach cancer risk. However, ERCC2 K751Q polymorphism was associated with an increased risk for non-cardial neoplasm [odds ratio (OR) = 1.78; 95% confidence interval (CI) 1.02-3.12], being ERCC2 K751Q and D312N polymorphisms associated

Overview Cancers originating in the esophagus, gastroesophageal junctions, and stomach constitute a major health problem worldwide. In the United States, 37,600 new diagnoses of and 25,150 deaths from upper gastrointestinal cancers were estimated in 2009. 1 A dramatic shift in the location of upper gastrointestinal tumors has occurred in the United States, 2 and changes in histology and location of them were observed in some parts of Europe. 3,4 In countries in the Western Hemisphere, the most common sites of gastric cancer are the proximal lesser curvature, cardia, and gastroesophageal junction. 2 These changing trends may also begin to occur in South America and Asia. Epidemiology Gastric cancer is rampant in many countries around the world. In Japan, it remains the most common type of cancer among men; in China, more new cases are diagnosed each year than in any other country. The incidence of gastric cancer, however, has been declining globally since World War II and it is one of the least ...

my mother was recently diagnosed w/same cancer (linitis plastica gastric cancer). she is also at stage 3b. I have not been able to find any stories of a survivor either, but believe that the current treatments can work (either for a while until better treatments are discovered or as a possible cure). I have done plenty of research on the subject and would be more than happy to discuss with you her treatment, etc...she is responding well to it and we are hopeful. please let me know if you would like to further discuss these issues. As you probably already know, linitis plastica is rare, so finding other patients is probably even more critical with this type of cancer, because information is scarce. I hope your wife is ok...I know what you are going through.. ...

div class=citation vocab=http://schema.org/,,i class=fa fa-external-link-square fa-fw,,/i, Data from ,span resource=http://link.library.in.gov/resource/OuMdz6KcJrU/ typeof=CategoryCode http://bibfra.me/vocab/lite/Concept,,span property=name http://bibfra.me/vocab/lite/label,,a href=http://link.library.in.gov/resource/OuMdz6KcJrU/,Stomach -- Tumors , Diagnosis,/a,,/span, - ,span property=potentialAction typeOf=OrganizeAction,,span property=agent typeof=LibrarySystem http://library.link/vocab/LibrarySystem resource=http://link.library.in.gov/,,span property=name http://bibfra.me/vocab/lite/label,,a property=url href=http://link.library.in.gov/,Indiana State Library,/a,,/span,,/span,,/span,,/span,,/div ...

TY - JOUR. T1 - Phase II study of biweekly docetaxel and S-1 combination therapy for advanced or recurrent gastric cancer. AU - Kakeji, Yoshihiro. AU - Oki, Eiji. AU - Egashira, Akinori. AU - Sadanaga, Noriaki. AU - Takahashi, Ikuo. AU - Morita, Masaru. AU - Emi, Yasunori. AU - Maehara, Yoshihiko. PY - 2009/7/1. Y1 - 2009/7/1. N2 - Objective: This phase II study evaluated the toxicity and efficacy of a novel dosing schedule of docetaxel and S-1 as treatment for advanced gastric cancer. Methods: Patients with measurable advanced or recurrent gastric cancer and no prior exposure to the investigational drugs were treated with intravenous docetaxel 35 mg/m2 on days 1 and 15, and oral S-1 80 mg/m 2/day on days 1-14 every 4 weeks. The primary endpoint was objective response. Results: Thirty-five eligible patients were enrolled and received a total of 151 cycles of treatment (median 3, range 1-19). One complete response and 13 partial responses were observed, with an overall response rate of 40% (95% ...

Since November 2006, at our Institute, selected patients affected by gastric adenocarcinoma were enrolled in a program of peri-operative chemotherapy; six of them were over 70 years old. The neoplasia was diagnosed and staged by gastroscopy, endoscopic ultrasonography and total body 18FDG-PET-CT. Inclusion criteria were: cT2N+M0 or cT3-4N × M0, age ,75, Karnofsky Performance Status ,60%, no hepatic, renal and bone marrow failure (creatinine ,1.5 mg/dL; clearance creatinine ,50 ml/L; total bilirubin ,2 mg/dl; white blood cells ,3500 mm3; platelets ,140000/mm3). The patient underwent three cycles of pre-operative chemotherapy with Epirubicine, Cisplatin and 5-Fluorouracil (ECF) as MAGIC Trial showed. After every cycle hepatic, renal, bone marrow and cardiac functionality were evaluated. Fifteen days after the third pre-operative ECF cycle the patients underwent endoscopic ultrasonography and total body 18FDG-PET-CT to evaluate the tumor response to chemotherapy, then they underwent surgery. ...

TY - JOUR. T1 - Highly advanced gastric cancer that responded to neoadjuvant combination chemotherapy with docetaxel/CDDP/S-1 (DCS). AU - Egawa, Tomohisa. AU - Kenmochi, Takeshi. AU - Ohashi, Masaki. AU - Irino, Tomoyuki. AU - Mihara, Koki. AU - Okamura, Akihiko. AU - Inaba, Yusaku. AU - Ito, Yasuhiro. AU - Hayashi, Shinobu. AU - Nagashima, Atsushi. PY - 2010/11. Y1 - 2010/11. N2 - A 60-year-old male was found to have advanced gastric cancer and multiple lymph node metastases. Since curative surgery was concluded to be unfeasible, we tried neoadjuvant chemotherapy with the aim of controlling the lymph node metastasis. S-1 (80 mg/m2) was administered orally for two weeks then followed by 2-week rest period. CDDP (60 mg/ m2) and docetaxel (40 mg/m2) were simultaneously administered on day 1. Two courses of treatment resulted in marked shrinkage of the primary lesion and a reduction in size of the lymph nodes. The results were evaluated as a clinical PR based on RECIST, and radical resection was ...