TY - JOUR. T1 - High-throughput real-time RT-PCR assay to detect the exotic Newcastle Disease Virus during the California 2002-2003 outbreak. AU - Crossley, Beate. AU - Hietala, Sharon K.. AU - Shih, Liu Mei. AU - Lee, Lou. AU - Skowronski, Evan W.. AU - Ardans, Alex. PY - 2005/3. Y1 - 2005/3. N2 - During the 2002-2003 Exotic Newcastle Disease (END) outbreak in Southern California, a high-throughput real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) system was developed to respond to the large diagnostic and surveillance sample workload. A 96-well RNA extraction method, using magnetic bead technology, combined with a 96-well RRT-PCR assay, allowed 1 technician to process and test more than 400 samples per day. A 3-technician team could complete testing on approximately 1,900 samples per day. The diagnostic sensitivity of the high-throughput RRT-PCR assay was 0.9967 (95% CI 0.9937-0.9997) based on 926 virus isolation confirmed positive samples. Diagnostic specificity using an ...
The hematological lesions consequent upon velogenic Newcastle disease virus (NDV) infection were investigated in 6-week-old ducks and chickens. Following intramuscular inoculation, the results indicated significantly lower (p
Newcastle disease (ND) is regarded as a highly contagious and economically important disease in poultry and has a worldwide distribution. Viral determinants for Newcastle disease virus (NDV) virulence are not completely understood and viruses of different pathotypes can be found at live-bird markets in different geographical areas. The prevalence of Newcastle disease in village poultry in Mozambique is not well documented and strains of NDV involved in yearly outbreaks are unknown. The fusion (F) protein is an important determinant of pathogenicity of the virus and is used commonly for phylogenetic analysis. Newcastle disease viruses from various geographical regions of Mozambique were sequenced and compared genetically to published sequences obtained from GenBank. Samples were collected in three different areas of Mozambique and NDV was isolated by infection of embryonated chicken eggs. Sequence analysis of the F-protein encoding gene was used to classify 28 isolates from Mozambique into ...
Newcastle disease virus infection in 38 weeks old layer brown chicken, mild respiratory signs, normal daily mortality rate, drop of egg production, change color egg, abnormal shape. Velogenic Newcastle disease virus is the deadliest virus for poultry. So many people assume, if this virus infects a chicken flock, then they […]. ...
Read "Existence of two forms of L protein of Newcastle disease virus isolates due to a compensatory mutation in Domain V, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Virulent Newcastle Disease (vND) is a poultry disease that has not been seen in California since 2002. It has recently re-emerged and represents a serious threat to backyard and commercial flocks. UC Cooperative Extension specialist Maurice Pitesky, who is affiliated with the UC Davis School of Vete
Newcastle disease virus (NDV) can cause severe disease in chickens. Although NDV vaccines exist, there are frequent reports of outbreaks in vaccinated chickens. During 2009-2010, despite intense vaccination, NDV caused major outbreaks among commercial poultry farms in Indonesia. These outbreaks raised concern regarding the protective immunity of current vaccines against circulating virulent strains in Indonesia. In this study, we investigated whether a recombinant attenuated Indonesian NDV strain could provide better protection against prevalent Indonesian viruses. A reverse genetics system for the highly virulent NDV strain Banjarmasin/010/10 (Ban/010) isolated in Indonesia in 2010 was constructed. The Ban/010 virus is classified in genotype VII of class II NDV, which is genetically distinct from the commercial vaccine strains B1 and LaSota, which belong to genotype II, and shares only 89 and 87% amino acid identity for the protective antigens F and HN, respectively. A mutant virus, named Ban/AF, was
Pullets about to enter a laying flock were revaccinated by intramuscular injection of live Mukteswar strain Newcastle disease virus, a procedure commonly used in Pakistan. Chicks hatched from eggs produced 24 weeks later were assessed for passively acquired immunity. Levels of haemagglutination-inhibition (HI) antibody and resistance to challenge with velogenic Newcastle disease virus were determined on groups of 50 chicks at 1, 4, 7, 10, 13, 16, 19, 22 and 25 days of age. Levels of maternally derived antibody declined from a geometric mean titre of 40 (25.3) on the day of hatching to less than two (20.3) at 25 days of age, with a decline of 1log2 in about 5 days. Mortalities after challenge varied from 18% at 1 day of age to 90% at 25 days of age. Survival was significantly related to the level of antibody at the time of challenge. None of 111 chickens with an antibody titre of 40 (25.3) or more succumbed to challenge. Mortality rates varied from 78.6% in chicks with no detectable antibody at ...
NDV is classified as an avian paramyxovirus-1 in the Avulavirus genus of the family Paramyxoviridae [13], whose genomes consist of negative-sense single-stranded RNA. This RNA contains six open reading frames that encode six structural proteins: the nucleoprotein, the phosphoprotein, the large polymerase protein, the matrix protein, HN and fusion glycoproteins [14]. The infection of host cells starts once the viral HN protein binds to its receptor [15]. Receptor recognition by HN triggers the activation of the fusion protein, which promotes the fusion of the viral and cell membranes and allows the entry of the ribonucleoproteins into the cytoplasm. Genome replication takes place in the cytoplasm and does not involve any intermediate DNA stages.. The rL-RVG strain was provided by the Harbin Veterinary Research Institute (Harbin, China); it is a recombinant NDV expressing RVG. Recent research has demonstrated that rL-RVG can spread between cells in a similar manner as the rabies virus does. ...
vND, formerly known as Exotic Newcastle Disease is a contagious and fatal viral disease affecting the respiratory, nervous and digestive systems of birds and poultry. The disease is so virulent that many birds and poultry die without showing any clinical signs.. vND is not a food safety concern. No human cases of Newcastle disease have ever occurred from eating poultry products. Properly cooked poultry products are safe to eat. In very rare instances people working directly with sick birds can become infected with mild symptoms.. vND has not been found in commercial poultry in the U.S. since 2003.. ...
A reverse genetics system using prevalent genotype VIId isolate SG10 was constructed and a mutant virus, named aSG10, was developed by changing the virulent F protein cleavage site motif "112RRQKR↓F117" into an avirulent motif "112GRQGR↓L117". The attenuated pathogenicity of aSG10 was confirmed from the mean death time and intracerebral pathogenicity index. aSG10 and LaSota both protected vaccinated birds from death after challenge with highly virulent genotype VII NDV, strain SG10. However, aSG10 significantly reduced the challenge virus shedding from the vaccinated birds compared to LaSota vaccine. We also generated a recombinant virus, aSG10-enhanced green fluorescent protein (EGFP), which expresses EGFP. aSG10-EGFP stably expressed EGFP for at least 10 passages. ...
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The first Newcastle disease virus (NDV) field strain (NDV-QC08), previously isolated and identified in Qatar, was further subjected to molecular characterization and phylogenic analysis at passage levels two. Initial amplification and detection of the complementary DNA (cDNA) fragment of the NDV RNA amnioallantoic fluid (AAF) extract demonstrated the positive virus identity using real-time reverse transcriptase chain reaction (RRT-PCR). Partial sequence of the totally amplified 216 bp of the functional cleavage region encoding the F gene retrieved 112R-R-Q-K-R-F117 amino acid motif indicating the velogenic nature of the strain, and confirming the previously investigated in-vivo pathotyping. Phylogenic analysis and genotypic clustering studies classified the strain under NDV subgenotype VIId. ...
This NebFact is about the Exotic Newcastle Disease (END) which is a foreign animal disease in the U.S. and is considered the most infectious, contagious, and fatal viral disease of birds and poultry.
The Animal and Plant Health Inspection Service (APHIS) is proposing to make several changes to the exotic Newcastle disease (END) domestic quarantine regulations. The proposed changes include harmonizing the foreign and domestic regulations regarding the movement of dressed carcasses of dead birds and dead poultry; adding restrictions on the interstate movement of ratites out of quarantined areas; providing for the use of alternative procedures for treating manure and litter for composting, and adding an additional surveillance period prior to removing quarantine restrictions ...
BACKGROUND: It is important to understand the efficacy of immunoregulatory materials, herbal remedies or probiotics, in different parts of immune system following vaccination with different tropism. OBJECTIVES: Aim of this study was to evaluate the effect of Echinacea purpurea and a probiotic (protexin) on systemic and mucosal immune response in turkey. METHODS: A total of 288 1-day-old male turkey poults were randomized into 6 groups as follow: Group T1: Turkeys received Echinacea purpurea at the rate of 1 ml /1 liter water and Newcastle disease virus (NDV) vaccine, Group T2: Turkeys received probiotic at the rate of 1 g /1 liter water and NDV vaccine, Group T3: Positive control that turkey received NDV vaccine without any additives. Group T4: Turkeys received Echinacea purpurea at the rate of 1 ml /1 liter water without NDV vaccine. Group T5: Turkeys received probiotic at the rate of 1 g /1 liter water without NDV vaccine, Group T6: Negative control group, neither vaccinated against NDV vaccine nor
During an investigation into the role of Indian house crows (Corvus splendens splendens) in the epizootology of Newcastle disease, a total of 164 samples from 82 crows were examined. Fifteen isolations of Newcastle diseases virus were made from 10 birds and one of these was highly pathogenic to chicken. Haemagglutination inhibition (HI) tests showed that 38 per cent of these crows possessed antibody to Newcastle disease virus. Initiation and duration of virus excretion and development of HI antibodies were also studied by experimental infection. Virus excretion began on day 3, continued till day 5 and complete elimination occurred by day 6. HI antibody titre began to rise from the seventh day to peak by the twenty-first day and declined thereafter.. ...
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Nutritional Enhancement of Soybean - Wendy Peer. The Peer Lab - Wendy Peer. Composting effective at reducing estrogens in broiler litter - Lance Yonkos and Gary Felton. Turning Poultry Litter to Energy with Enhanced Nutrient Recovery: Overview of Anaerobic Digestion, Gasification and Fluidized Bed Combustion Projects - Stephanie Lansing, Gary Felton, Freddy Witarsa, Amro Hassanein, Rohan Tikekar and Jonathan Moyle. Optimizing phosphorus digestibility through calcium management. The importance of digestible calcium - Roselina Angel. Metabolic and hormonal effects of heat stress in broiler chickens - Tom Porter. The importance of UV light for broiler well-being - Rachel Lynn Dennis. A recombinant Newcastle disease virus (NDV) expressing S gene of infectious bronchitis virus (IBV) protects chickens against virulent IBV and NDV challenges - Edris Shirvani, Anandan Paldurai, Vinoth Manoharan, Berin Parambethu Varghese and Siba K. Samal. A recombinant Newcastle disease virus (NDV) expressing gD gene ...
According to a study, a recombinant Newcastle disease virus kills all kinds of prostate cancer cells, including hormone resistant cells, but leaves normal cells unscathed.
Members of the Avulavirus, Respirovirus and Rubulavirus genera of the Paramyxoviridae family of viruses utilise haemagglutinin-neuraminidase glycoproteins as their attachment proteins. These glycoproteins are oligomeric type II integral membrane proteins, which possess haemagglutination and sialidase activity. Previous studies have shown that the N-linked glycans present on these proteins can modulate the ability of the virus to infect host cells and stimulate the host immune system. However, site-specific heterogeneity of these glycans has not been defined. This study concerns characterisation of the glycan compositions attached to haemagglutinin-neuraminidase of the Avulavirus Newcastle disease virus, which causes Newcastle disease in a range of avian species. Haemagglutinin-neuraminidase was derived from egg propagated virions of V4-VAR, an isolate of the avirulent strain QLD/66. Reverse-phase liquid chromatography tandem mass spectrometry strategies including collision induced dissociation, ...
Newcastle disease virus (NDV) causes severe diseases in avian species. Its fusion protein cleavage site (Fcs) is a major contributor to virulence and membrane fusion. Previous studies showed that a change from phenylalanine (F) to lysine (L) at position 117 of the virulent strain fusion protein, which has the polybasic amino acid Fcs motif
I have money for a graduate research assistantship through the Department of Medical Microbiology in the College of Veterinary Medicine at the University of Georgia at the MS or entry PhD level. The project involves examing virulent exotic Newcastle disease virus isolates by RT-PCR coupled to automated nucleotide sequencing correlated to standard pathotyping assays. The student must apply and be accepted to the graduate school and Department of Medical Microbiology at UGA. Funding is through grant funds with liberal supply and travel money. Student support will be via a research support agreement between the USDA and UGA. The standard graduate stipend will be available with tuition waiver. For further information please contact me at: Bruce S. Seal, PhD Southeast Poultry Research Lab, USDA 934 College Station Rd. Athens, GA 30605 706-546-3463 or -3434 (ph) -3161 (fax) bseal at asrr.arsusda.gov ...
This memorandum serves to notify regulated Canadian veterinary biologics manufacturers and importers of the requirement for filing intracerebral pathogenicity index (ICPI) test results for all master seed viruses that are used to produce live attenuated Newcastle Disease (ND) virus vaccines.
Exotic Newcastle Disease Virus (ENDV) cause extremely rapid mortality in chickens after exposure to the virus. People rely heavily on poultry to provide protein and income in many places where NDV is not effectively controlled through vaccination and biosecurity. Losses from NDV contribute to worldwide hunger and poverty. It may be possible to use genetic selection to produce chickens that have a stronger immune response in the face of NDV challenge. For genetic selection to be successful, two major elements are required: differences in immune response among chickens and genetic control of these differences. This study clearly demonstrated the existence of both these factors. These findings demonstrate the feasibility of genetic selection to produce chickens that are more resistant to NDV and thereby lessen the burdens of hunger and poverty.
Newcastle disease virus (NDV) is one of the most important infectious agents in the poultry industry [1]. NDV is synonym of avian paramyxovirus 1 (APMV-1), a member of the genus Avulavirus in the family Paramyxoviridae[2]. The enveloped virus has a negative-sense, single-stranded RNA genome of 15186, 15192, or 15198 nucleotides (nts) in length, encoding six proteins: nucleocapsid protein (NP), phosphoprotein (P), matrix protein (M), fusion protein (F), hemagglutinin-neuraminidase (HN), and large protein (L) [3-5]. Phylogenetically, NDV is divided into two distinct classes (I and II). Almost all virulent NDVs belong to class II which can be subdivided into sublineages and genotypes: early sublineage of genotypes I-IV with genome length of 15186 nts, late sublineage of genotypes V-VIII with genome length of 15192 nts and genotype IX belonging to early sublineage but having a genome length of 15192 nts [6-9].. The pathogenicity of NDV isolates can be assessed by determining the mean death time ...
Newcastle disease is a devastating viral disease of chicken in low- and middle-income countries where the backyard production system is predominant. Marker-assisted selection of chickens that are resistant to Newcastle disease virus (NDV) is the promising strategy that needs to be explored. )e aim of the present study was to investigate polymorphisms of the promoter region of the chicken Mx gene and association with Kuroiler, Sasso, and local Tanzanian chicken embryos survival variability to virulent NDV infection. Chicken embryos were initially challenged with a minimum lethal dose of virulent NDV suspension and then were followed over time to gather information on their survival variability. Using the survival data, high and less susceptible cohorts were established, and a total of 88 DNA samples from high and less susceptible groups were genotypes by sequencing. Five singlenucleotide polymorphisms (SNPs), which were previously reported, were detected. Interestingly, for the first time, the ...
TY - JOUR. T1 - Conserved, breed-dependent, and subline-dependent innate immune responses of Fayoumi and Leghorn chicken embryos to Newcastle disease virus infection. AU - Schilling, Megan A.. AU - Memari, Sahar. AU - Cavanaugh, Meredith. AU - Katani, Robab. AU - Deist, Melissa S.. AU - Radzio-Basu, Jessica. AU - Lamont, Susan J.. AU - Buza, Joram J.. AU - Kapur, Vivek. PY - 2019/12/1. Y1 - 2019/12/1. N2 - Newcastle disease virus (NDV) is a threat to the global poultry industry, but particularly for smallholder farmers in low- and middle-income countries. Previous reports suggest that some breeds of chickens are less susceptible to NDV infection, however, the mechanisms contributing to this are unknown. We here examined the comparative transcriptional responses of innate immune genes to NDV infection in inbred sublines of the Fayoumi and Leghorn breeds known to differ in their relative susceptibility to infection as well as at the microchromosome bearing the major histocompatability complex ...
Portions of California, Nevada, Arizona, and Texas remained under quarantine as a result of outbreaks of exotic Newcastle disease
Following the epidemiological surveillance activities on notifiable avian influenza within the national poultry farming and in poultry for import, low pathogenic avian influenza subtype H7N3 virus has been identified in a lot of monk parakeets (Myiopsitta M. monachus) imported from South America, using virus identification test, virus isolation, intracerebral pathogenicity index (ICPI) and gene sequencing ...
The hemagglutinin-neuraminidase (HN) protein of paramyxoviruses is likely in the unusual class of glycoproteins with the amino terminus cytoplasmic and the carboxy terminus lumenal or external to the cell. The properties of the membrane insertion of the HN protein of Newcastle disease virus, a prototype paramyxovirus, were explored in wheat germ extracts containing microsomal membranes. HN protein was inserted into membranes cotranslationally, resulting in a glycosylated protein completely resistant to trypsin and proteinase K digestion. No detectable posttranslation insertion occurred. Insertion required signal recognition particle. Signal recognition particle in the absence of membranes inhibited HN protein synthesis. Comparisons of the trypsin digestion products of the HN protein made in the cell-free system with newly synthesized HN protein from infected cells showed that the cell-free product was in a conformation different from that of the pulse-labeled protein in infected cells. First, trypsin
[...] the booty of Operation Gobble Gobble was destined for the industrial garbage disposal and left the cramped office filled with the sweet aroma of ground citrus. At this time of the year, we really do try to raise the awareness of the traveling public on the potential of introducing a pest or disease that could be damaging to American agriculture, said Diana Vlasik, the agencys chief agriculture specialist at the international bridges in Pharr and Hidalgo, about 150 miles southwest of Corpus Christi. Among the threats are the Mexican fruit fly; exotic Newcastle disease, which is fatal to poultry; and bacteria that causes citrus greening, which has ravaged groves in Florida. [...] from Thanksgiving through the New Years, the border is jammed with less-experienced travelers visiting relatives in Mexico or the U.S., as well as those who know better but are willing to risk confiscation and a fine trying to deliver key ingredients for a Christmas punch or tamales. At the Eagle Pass port of entry,
My current students are working on various second language topics including L2 phonology of French, Japanese; acquisition of L2 English articles; input in a TELL environment; early reading by low-educated adults; extensive reading; writing simply cracking good stories.. Students who have completed their PhDs under my supervision are:. Eye-tracking of Arabic and Chinese L2 English learners morphosyntax Walid Kahoul (Newcastle 2014) Arabic and Chinese speakers acquisition of binding in L2 English, Amer Al Kafri (Newcastle 2013) Multi-competence in German, Petra Schoofs (Newcastle 2013). Child L2 English phonology in Hong Kong, Alex Leung (Newcastle 2012). Working memory and complex syntax in Chinese L2 English, Clare Wright (Newcastle 2011) Instructed L2 French morphosyntax, Vivienne Rogers (Newcastle 2011). The pragmatics of L2 misunderstandings, Enas El-Sadek (Newcastle 2010) Extensive reading in Taiwan in primary English, I-Ching Chiang (Newcastle 2009 ...
Computer fileSeries: Applied and Environmental Microbiology.Publisher: American Society for Microbiology, 2013Description: Journal article.ISSN: 1098-5336.Online resources: Link to original article. In: Applied and Environmental Microbiology (Vol.) 79. (No.) 2. 2013. (Pages.) 639-645.Summary: Newcastle disease virus (NDV) is one of the most important viral diseases of birds. Wild birds constitute a natural reservoir of low-virulence viruses, while poultry are the main reservoir of virulent strains. Exchange of virus between these reservoirs represents a risk for both bird populations. Samples from wild and domestic birds collected between 2006 and 2010 in Luxembourg were analyzed for NDV. Three similar avirulent genotype I strains were found in ducks during consecutive years, suggesting that the virus may have survived and spread locally. However, separate introductions cannot be excluded, because no recent complete F gene sequences of genotype I from other European countries are available. ...
The Lasher Laboratory enhances the University of Delawares ability to respond quickly and efficiently to industry needs for poultry disease diagnosis and outreach activities. Diagnostic services available at the Lasher Laboratory include poultry necropsy and clinical diagnosis, bacteriology, mycology, molecular diagnostic testing, and serology. Virology and histopathology specimens collected at the laboratory are sent to the Newark campus for evaluation. The Lasher Laboratory conducts applied research on poultry diseases, often in collaboration with poultry industry scientists, and provides a broiler progeny challenge service for evaluating infectious bursal disease virus vaccinal immunity. As a NALHN approved laboratory, the Lasher Laboratory routinely performs surveillance for avian influenza and exotic Newcastle disease. Avian influenza surveillance programs include pre-slaughter testing of commercial broiler chickens, routine surveillance of backyard flocks, and testing of diseased flocks. ...
Define coals to Newcastle. coals to Newcastle synonyms, coals to Newcastle pronunciation, coals to Newcastle translation, English dictionary definition of coals to Newcastle. Something brought or sent to a place where it is already plentiful; it is a reference to the English town of Newcastle upon Tyne, historically a major coal...
A surveillance program directed specifically at detecting Classical Swine Fever is now in progress at Iowa State University Veterinary Diagnostic Laboratory (ISU VDL). The ISU VDL is one of the 12 core laboratories in the National Animal Health Laboratory Network (NAHLN). The primary function of NAHLN labs is to participate in ongoing surveillance and response to foreign animal diseases and other events requiring an integrated laboratory effort of preparedness, controlled testing, and timely reporting. Federal funding for this program provides equipment and diagnostic reagents as well as support for technical positions and proficiency training of technicians. The ISU VDL has the in-house capability to test for Classical Swine Fever, High Pathogenic Avian Influenza and Exotic Newcastle Disease.. Diagnosticians at the ISU VDL will select suspect cases from routine submissions to the ISU VDL. The criteria listed below will be used in case selection.. ...
The clinic is a joint Newcastle University and Newcastle Hospitals NHS Foundation Trust venture. It provides group and individual speech and language therapy for children in Newcastle.. It also helps children referred to the Paediatric Speech and Language Therapy Service. Newcastle General Hospital host this service.. We select children for the clinic. This ensures they benefit and make the best progress from the service we provide.. Students provide the therapy, supervised by the Universitys Speech and Language Therapists.. When you bring your child here, you are helping them. But you are also making an important contribution to training future therapists.. ...
Abstract:. The goal of this project is to evaluate efficacy of two vectored vaccines (Newcastle Disease Virus-vectored and vaccinia virus-vectored vaccines) for porcine epidemic diarrhea (PED) and classical swine fever (CSF). This project includes 3 specific objectives: 1) Determine immunogenicity and immunization approach (prime/boost) using vaccinia virus (VACV)-vectored and Virus (NDV)-vectored PED vaccines in pigs; 2) Evaluate efficacy of the optimized approach using NDV-vectored and VACV-vectored PED vaccines in pregnant sows; 3) Evaluate efficacy of NDV-vectored and VACV-vectored CSF vaccines in pigs. In this proposal, we plan to evaluate the immunogenicity and/or efficacy of NDV-based and VACV-COP-based PED and CSF vaccines against each pathogen in pigs. The results from these studies will provide insight on whether both vectored vaccines are able to provide protection in pigs against virus challenge, and will enhance our knowledge regarding development of effective vaccines for these ...
Chitosan-coated poly(lactic-co-glycolic) acid nanoparticles as an efficient delivery system for Newcastle disease virus DNA vaccine Kai Zhao,1,* Yang Zhang,1,2,* Xiaoyan Zhang,1,* Ci Shi,1,2 Xin Wang,1 Xiaohua Wang,1 Zheng Jin,3 Shangjin Cui2 1Laboratory of Microbiology, School of Life Science, Heilongjiang University, 2Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, 3Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, Heilongjiang University, Harbin, People’s Republic of China *These authors contributed equally to this work Abstract: We determined the efficacy and safety of chitosan (CS)-coated poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) as a delivery system for a vaccine to protect chickens against Newcastle disease virus (NDV). The newly constructed vaccine contained DNA (the F gene) of NDV. The Newcastle disease virus (NDV) F gene deoxyribonucleic acid (DNA)
We perform active monitoring of viruses in migratory birds. I was looking for Newcastle disease virus, a type 1 avian paramyxovirus, and my colleague Jansen de Araújo aimed to detect avian influenza virus for his research project. Finally, we found a co-infection by two viruses, one of which was totally unknown until then," said Luciano Matsumiya Thomazelli, a researcher at ICB-USPs Clinical & Molecular Virology Laboratory.. Since 2005, the lab team has been going into the field to perform epidemiological surveillance in different parts of Brazil as part of the Viral Genetic Diversity Network (VGDN), funded by FAPESP and headed by Professor Edison Luiz Durigon.. Their goal is to detect the presence of avian influenza virus and Newcastle disease virus, among others, in birds and other wild animals. In addition to monitoring, they aim to assess the risk of new strains arriving in Brazil. The samples collected by the team are stored in freezers at -80 °C and used in scientific research.. The new ...
The city has a strong sporting tradition. Football club Newcastle United has been based at St James Park since the club was established in 1892, although any traces of the original structure are now long gone as the stadium now holds more than 52,000 seated spectators, being Englands fourth largest football stadium.[218] The city also has non-League football clubs, Newcastle Benfield, West Allotment Celtic and Team Northumbria.. The Newcastle Falcons are the only rugby union team in north-east England to have played in the Aviva Premiership. They play at Kingston Park Stadium in the northern suburb of Kingston Park. 1996 Pilkington Shield winners Medicals RFC are also based in Newcastle.. Newcastle Thunder (formerly Gateshead Thunder) are a professional rugby league club based in the city who now also play at Kingston Park Stadium. They currently play in the Kingstone Press League 1. Since 2015, the Super League Magic Weekend has been played annually in the city at St James Park.. There is a ...
Windhoek. More than 4 000 chickens have already died since the outbreak of Newcastle disease in the northern communal areas (NCAs) in August. The outbreak is still confined to the four regions of Omusati. Oshana, Ohangwena and Oshikoto but no new cases have been reported outside these regions.. Updating all poultry farmers and the public yesterday, the acting permanent secretary in the agriculture ministry, Sophia Kasheeta, said the ban on movement and trading of live birds (chickens, ducks, ostriches, guinea fowls, caged birds, pigeons, doves and others), uncooked eggs, feathers and chicken feed originating from establishments where live chickens are kept, within and from the infected regions, is still in place.. "Movement of live birds and hatching eggs from other countries or regions south of the veterinary cordon fence into the infected regions has also been banned with immediate effect, and all import permits issued prior to this announcement are hereby withdrawn," she noted.. Kasheeta said ...
The fusion (F) and hemagglutinin-neuraminidase (HN) glycoproteins of the paramyxovirus simian virus 5 (SV5) were expressed individually or coexpressed in CV-1 cells by using SV40-based vectors and recombinant vaccinia viruses. The extent of detectable fusion in a syncytium formation assay was found to be affected by the expression system used. In addition, when HN was coexpressed with F, it was found that the expression vector system influenced the contribution of HN in forming syncytia. The abilities of the SV5, human parainfluenza virus type 3, and Newcastle disease virus F glycoproteins to cause fusion, when expressed alone or coexpressed with HN, were directly compared by using the SV40-based vector system in CV-1 cells. The F proteins exhibited various degrees of fusion activity independent of HN expression, but the formation of syncytia could be enhanced to different extents by the coexpression of the homotypic HN protein. ...
BA (Hons) Ph.D. DipM DipMRS PGCTL FRSA FHEA MBGS FCIM Chartered Marketer. Amanda is Chief Executive Officer at Newcastle University London and has held Chairs in Marketing at multiple institutions in the UK and Visiting Chairs internationally. An eminent social scientist, she has a strong leadership track record and rare insight into a cross-section of strategic groupings of Universities. Before moving to Newcastle, she was Pro-Vice Chancellor (International Priorities) and Executive Dean of the College of Business & Law, University of Salford; Principal of St. Cuthberts Society (A Durham University College) and Deputy Dean of Durham Business School. Amanda has a strong profile of forging successful collaborations within world-class higher education and business, and a high-achievement track record in business change and innovation. Her award-winning leadership in multi-disciplinary pedagogical innovation, technology-enhanced learning and embedding global employability within the curriculum has ...
Philip van der Eijk is Visiting Research Professor of Greek at Newcastle University. He was born in Delft, the Netherlands, and read Classics at Leiden University, where he received his doctorate in 1991 and where he held a Postdoctoral Fellowship funded by the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) from 1991 to 1994. In 1994, he moved to Newcastle University to take up a Wellcome Trust University Award in the History of Medicine in the Classical World. At Newcastle, he was appointed (Personal) Professor of Greek in 1998, and Research Professor of Greek in 2005. With colleagues from Newcastle and Durham, he founded the Northern Centre for the History of Medicine in 2003, of which he was a Director until 2009. As of January 2010, he is Alexander von Humboldt Professor of Classics and History of Science at the Humboldt University in Berlin. ...
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The limited efficacy of current treatments against pancreatic cancer has prompted the search of new alternatives such as virotherapy. Activation of the immune response against cancer cells is emerging as one of the main mechanisms of action of oncolytic viruses (OV). Direct oncolysis releases tumor antigens, and viral replication within the tumor microenvironment is a potent danger signal. Arming OV with immunostimulatory transgenes further enhances their therapeutic effect. However, standard virotherapy protocols do not take full advantage of OV as cancer vaccines because repeated viral administrations may polarize immune responses against strong viral antigens, and the rapid onset of neutralizing antibodies limits the efficacy of redosing. An alternative paradigm based on sequential combination of antigenically distinct OV has been recently proposed. We have developed a protocol consisting of sequential intratumor administrations of new Adenovirus (Ad) and Newcastle Disease Virus (NDV)-based OV
An oncolytic virus is a virus that preferentially infects and kills cancer cells. As the infected cancer cells are destroyed by oncolysis, they release new infectious virus particles or virions to help destroy the remaining tumour. Oncolytic viruses are thought not only to cause direct destruction of the tumour cells, but also to stimulate host anti-tumour immune responses. The potential of viruses as anti-cancer agents was first realised in the early twentieth century, although coordinated research efforts did not begin until the 1960s. A number of viruses including adenovirus, reovirus, measles, herpes simplex, Newcastle disease virus and vaccinia have now been clinically tested as oncolytic agents. Most current oncolytic viruses are engineered for tumour selectivity, although there are naturally occurring examples such as reovirus and the senecavirus, resulting in clinical trials. The first oncolytic virus approved by a national regulatory agency is genetically not modified ECHO-7 strain ...
1. Christine L. Fleischacker, Miriam Segura-Totten, SEA-PHAGES, Rebecca A. Garlena, Deborah Jacobs-Sera, Welkin H. Pope, Daniel A. Russell, and Graham F. Hatfull. 2017. Genome Sequence of Mycobacterium Phage CrystalP. GenBank: KY319168.1 https://www.ncbi.nlm.nih.gov/nuccore/KY319168. 2. Haddad, E. E. 2016. A Message from the Immune System: A Vaccine Technology in Needs of Exploration! International Journal of Vaccines and Vaccination, 2(4): 00038. http://medcraveonline.com/IJVV/volume_issues?issueId=768&volumeId=248 3. Senter, P., Mattox, U., and Haddad, E. E. 2016. Snake to monster: Conrad Gessners Schlangenbuch and the evolution of the dragon in the literature of natural history. J. Folklore Research 53:67-124. http://muse.jhu.edu/article/610975. 4. Darrell R. Kapczynski, Alison Martin, Eid E. Haddad, Daniel J. King. 2012. Protection from Clinical Disease Against Three Highly Virulent Strains of Newcastle Disease Virus Following In Ovo Application of an Antibody-Antigen Complexed Vaccine in ...
A strain each of mumps and Newcastle disease virus and five strains of influenza virus were found to be capable of removing all the receptors for this group of viruses from fowl red cells. Five virus strains were tested for their capacity to inactivate the virus hemagglutinin of human plasma and of egg white. In the case of egg white all strains including mumps and Newcastle disease virus inactivated the inhibitor completely, or nearly so. With plasma the influenza strains inactivated the inhibitor completely but mumps and NDV destroyed only that portion of the complex which effected mumps inhibition. The inhibitor for some strains was destroyed more rapidly than that for others and the sequence in which they were destroyed (inhibitor gradient) was similar, regardless of the strain employed. The inhibitor gradient for egg white was very different from that for plasma and these in turn differed significantly from the receptor gradient for fowl red cells.. ...
BLACKSBURG, Va., April 8, 2013 - A study at the Virginia-Maryland Regional College of Veterinary Medicine has identified a chicken-killing virus as a promising treatment for prostate cancer in humans.. Researchers have discovered that a genetically engineered Newcastle disease virus, which harms chickens but not humans, kills prostate cancer cells of all kinds, including hormone-resistant cancer cells. The work of Dr. Elankumaran Subbiah, associate professor of virology in the Department of Biomedical Sciences and Pathobiology, along with Dr. Siba Samal, associate dean and chairman of the University of Marylands Department of Veterinary Medicine, and Shobana Raghunath, a graduate student in Subbiahs laboratory, appears in the April 2013 issue of the Journal of Virology.. "This potential treatment is available for immediate pre-clinical and clinical trials, but these are typically not done at the university level," Subbiah said. "We are looking for commercial ...
These are interactions for viagra simple rank-based data augmentation techniques, which formalize the use of funnel plots. Oral healthcare systems (OHCS) are designed to maintain the health and function through Communication (Health promotion and education), Prevention, Assessment and Diagnosis, and Treatment. Cross-sectoral optimization and visualization of transformation processes in urban water infrastructures in rural areas.. Classification of acute myeloid leukemias-a comparison of FAB and immunophenotyping. CT scan natural viagra revealed a multicystic lesion of the pancreatic head, which caused biliary obstruction. Cases 2 and 3 were complicated by the high flow variant of priapism.. Protein components of generic for viagra classical fowl plague virus and Newcastle disease virus Together, these data demonstrate that syntaxin 6 utilizes two distinct domains to drive its specific subcellular localization to the TGN.. Although globin chains were good rx sildenafil clearly present in the ...
Oncolytic virotherapy has been an attractive drug platform for targeted therapy of cancer over the past few years. Viral vectors can be used to target and lyse cancer cells, but achieving good efficacy and specificity with this treatment approach is a major challenge. Here, we assessed the ability of a novel dual-specific anti-tumor oncolytic adenovirus, expressing the hemagglutinin-neuraminidase (HN) gene from the Newcastle disease virus under the human telomerase reverse transcriptase (hTERT) promoter (Ad-hTERTp-E1a-HN), to inhibit esophageal cancer EC-109 cells in culture and to reduce tumor burden in xenografted BALB/c nude mice. In vitro, infection with Ad-hTERT-E1a-HN could inhibit the growth of EC-109 cells significantly and also protect normal human liver cell line L02 from growth suppression in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Ad-hTERT-E1a-HN also effectively and selectively decreased the sialic acid level on EC-109 cells, but not on L02 cells.
A settlement was again attempted in 1804, as a place of secondary punishment for unruly convicts. The settlement was named Coal River, also Kingstown and then renamed Newcastle, after Englands famous coal port.[9] The name first appeared by the commission issued by Governor King on 15 March 1804 to Lieutenant Charles Menzies of the marine detachment on HMS Calcutta, then at Port Jackson, appointing him superintendent of the new settlement.[12] The new settlement, comprising convicts and a military guard, arrived at the Hunter River on 27 March 1804 in three ships: HMS Lady Nelson, the Resource and the James.[10][13] The convicts were rebels from the 1804 Castle Hill convict rebellion. The link with Newcastle upon Tyne, England its namesake and also whence many of the 19th century coal miners came, is still obvious in some of the place-names - such as Jesmond, Hexham, Wickham, Wallsend and Gateshead. Morpeth, New South Wales is a similar distance north of Newcastle as Morpeth, Northumberland is ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Citation: Chmielewski, R.A., Swayne, D.E. 2010. Thermal inactivation of Newcastle virus in imitation egg product [abstract]. Southeastern Regional Virology Conference, March 19-21, 2010, Atlanta, Georgia. p. 4. Interpretive Summary: Technical Abstract: Exotic Newcastle Disease is an avian disease caused by avian paramyxovirus (APMV-1). The virulent form of this virus is 100% fatal in unvaccinated birds while low virulent forms may cause asymptomatic infection to respiratory disease. This disease has been economically devastating to the poultry industry worldwide. With the increase in global trade, there are concerns of that some foods could potentially present biosecurity problems and affect trade agreements. The World Organization for Animal Health (OIE) and the American Egg Board have requested the evaluation of pasteurization processes to inactivate APMV-1. We selected imitation eggs to evaluate the effectiveness of U.S. pasteurization standards for egg products to inactivate a low virulent ...
The Ulster Special Constabulary (USC; commonly called the "B-Specials" or "B Men") was a quasi-military reserve special constable police force in Northern Ireland. It was set up in October 1920, shortly before the partition of Ireland. It was an armed corps, organised partially on military lines and called out in times of emergency, such as war or insurgency. It performed this role in 1920-22 during the Irish War of Independence and in the 1950s, during the IRA Border Campaign. During its existence, 95 USC members were killed in the line of duty. Most of these (72) were killed in conflict with the IRA in the years 1921 and 1922. Another 8 died during the Second World War, in air raids or IRA attacks. Of the remainder, most died in accidents but two former officers were killed during the Troubles in the 1980s. The force was almost exclusively Ulster Protestant and as a result was viewed with great mistrust by Catholics. It carried out several revenge killings and reprisals against Catholic ...
Compare social security disability/ssi attorneys in Ulster County, New York on Justia. Comprehensive profiles including fees, education, jurisdictions, awards, publications and social media.
Charity Choice list of charities includes Herts Aid and other Specific Conditions charities. Herts Aid in East is featured in the Health charity database on Charity Choice.
We have analyzed the mechanism by which M protein interacts with components of the viral envelope during Sendai virus assembly. Using recombinant vaccinia viruses to selectively express combinations of Sendai virus F, HN, and M proteins, we have successfully reconstituted M protein-glycoprotein interaction in vivo and determined the molecular interactions which are necessary and sufficient to promote M protein-membrane binding. Our results showed that M protein accumulates on cellular membranes via a direct interaction with both F and HN proteins. Specifically, our data demonstrated that a small fraction (8 to 16%) of M protein becomes membrane associated in the absence of Sendai virus glycoproteins, while , 75% becomes membrane bound in the presence of both F and HN proteins. Selective expression of M protein together with either F or HN protein showed that each viral glycoprotein is individually sufficient to promote efficient (56 to 73%) M protein-membrane binding. Finally, we observed that M ...
At the present time virus grown in one layer tissue culture is successfully used for preparing deactivated antifoot-and-mouth disease vaccine. This article discusses the effect of some conditions on the multiplication foot-and-mouth disease virus in a tissue culture of pig embryo kidney cells (PEK). The article discusses the materials and methods used in the study and the results of the study, contains a discussion of the results, and makes the following conclusions: (1) The multiplication of foot-and-mouth disease virus in PEK tissue culture does not depend on preliminary adsorption of virus on cells. (2) Growing foot-and-mouth disease virus in PEK culture for the preparation of vaccine is a promising method. (Author)*TISSUE CULTURE)
We worked in the Centre for Bacterial Cell Biology (CBCB) at Newcastle University for the entire project, where there are clearly defined safety rules and regulations that all laboratory workers must follow. One of our advisors, Dr Wendy Smith, guided us through the safety procedures along with some of the basic techniques during the first introductory week, before any laboratory work on the project began. This included carrying out the following risk assessments to determine what control measures would be required. (i)Chemical Hazards: At the beginning of the project written risk assessments were already available in the host laboratories for all procedures that involved potentially hazardous chemicals. These risk assessments were reviewed and the recommended control measures were strictly followed throughout. No additional chemical hazards specific to this project were identified. (ii)Radioisotopes and carcinogens: None of these were used in this project. (iii)Biological hazards: Throughout ...
Millions of laboratory animals are killed each year worldwide. There is an ethical, and in many countries also a legal, imperative to ensure those deaths cause minimal suffering. However, there is a lack of consensus regarding what methods of killing are humane for many species and stages of development. In 2013, an international group of researchers and stakeholders met at Newcastle University, United Kingdom to discuss the latest research and which methods could currently be considered most humane for the most commonly used laboratory species (mice, rats and zebrafish). They also discussed factors to consider when making decisions about appropriate techniques for particular species and projects, and priorities for further research. This report summarises the research findings and discussions, with recommendations to help inform good practice for humane killing.
Millions of laboratory animals are killed each year worldwide. There is an ethical, and in many countries also a legal, imperative to ensure those deaths cause minimal suffering. However, there is a lack of consensus regarding what methods of killing are humane for many species and stages of development. In 2013, an international group of researchers and stakeholders met at Newcastle University, United Kingdom to discuss the latest research and which methods could currently be considered most humane for the most commonly used laboratory species (mice, rats and zebrafish). They also discussed factors to consider when making decisions about appropriate techniques for particular species and projects, and priorities for further research. This report summarises the research findings and discussions, with recommendations to help inform good practice for humane killing.
CURRICULUM VITAE Ammarin Thakkinstian Work Address: Section for Clinical Epidemiology and Biostatistics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama VI Road, Rachatevi, Bangkok 10400, Thailand Work Telephone Number: 6622011762 Work Facsimile: 6622011284 Date of Birth: Gender: Citizenship: December 07, 1964 Female Thai Electronic Mail: [email protected] Academic Qualifications: 1988 B.Sc.(Nursing), School of Nursing, Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok Thailand 1993 M.Sc. (Biostatistics) Department of Biostatistics, Faculty of Public Health, Mahidol University Bangkok Thailand 1998 Master of Medical Statistics, Centre for Clinical Epidemiology and Biostatistics, Faculty of Medicine and Health Sciences, The University of Newcastle, Newcastle, NSW, AU 2000-2004 Ph.D. in Community Medicine and Clinical Epidemiology, Centre for Clinical Epidemiology and Biostatistics, School of Medicine and Health Sciences The University of Newcastle, Newcastle, ...
I qualified in medicine from the Medical School, Jagiellonian University in Cracow in 1983.. Between 1988 and 2005 I have been employed in the Department of Radiology, University Childrens Hospital in Cracow where I have been trained in paediatric radiology. I have completed fellowship in paediatric radiology in Graz (Austria) in 1995.. In 2003 I was awarded the title of PhD by Jagiellonian University in Cracow, upon preparing a dissertation on the diagnostic value of ultrasound examinations in appendicitis in children.. In February 2005 I commenced my employment as locum consultant in the Department of Radiology in Royal Victoria Infirmary, Newcastle upon Tyne Hospital Trust.. Since 2006 till now I continue my employment as Consultant Radiologist in the Radiology Department in the RVI.. My daily duties include conducting, interpreting and reporting imaging examinations of all modalities in children and young people. I am actively involved in the supervision, teaching and assessment of ...
Newcastle based Sintons can make an asbestos personal injury compensation claim if you have suffered illness as a result of your employment in shipyards
Foot-and-Mouth Disease (FMD) is a highly contagious infectious viral disease of livestock, which exhibits antigenic variation and is of great economic importance worldwide. Wildlife particularly the African buffalo (Syncerus caffer) is important maintenance hosts for foot-and-mouth disease virus (FMDV). The determination of prevalent antigenic types in domestic animals and wildlife; their relationship with vaccine strains is important in vaccine selection and success of vaccination programs. Serological methods, such as virus neutralization test (VNT) and the enzyme-linked immunosorbent assay (ELISA) have been used to subtype FMDV strains for epidemiological and vaccine suitability determination. In this study, sixty-eight FMDV isolates held at the FMD Laboratory, Embakasi, from some susceptible wild and domestic animal species were compared serologically with seven vaccine strains produced by the Kenya Veterinary Vaccines Production Institute (KEVEVAPI) and currently in use for FMD control in ...
Poultry can be affected by a variety of diseases and parasites.. The two most serious diseases that must be kept out of poultry flocks are Newcastle disease and avian influenza. Although these two devastating diseases are not present in commercial poultry in Australia, the poultry industry is at risk from their introduction.. Other poultry diseases include coryza, chronic respiratory disease, infectious laryngotracheitis, lice and mite infestations, chlamydiosis, blackhead and internal parasites.. A strict hygiene program is required to keep diseases out of poultry. Some diseases are controlled by vaccination or medication strategies.. ...
The official medicines control laboratory in an EU country inspects the production and testing documentation of the manufacturer of the veterinary vaccine and certifies the vaccine before it is released on the market.. Evira tests a part of the vaccine batches in the marketplace every year. In addition, a vaccine can be tested by using various tests whenever there is suspicion that a vaccine has caused adverse reactions for an animal or when the efficacy of the vaccine has not been as expected.. Evira does not test all batches but focuses on vaccines that are deemed to constitute the greatest threat to the status of animal diseases in Finland. For example, Finland is free of Newcastle disease in poultry, so live poultry vaccines are tested for the presence of this virus. Any spreading of the virus vaccine to poultry could jeopardise freedom from disease and incur significant costs.. Few veterinary vaccines for sale in Finland ...
Laird, E., Molloy, A. M., McNulty, Helene, Ward, Mary, McCarroll, K., Hoey, Leane, Hughes, Catherine, Cunningham, C., Strain, JJ and Casey, M. C. (2017) Greater yogurt consumption is associated with increased bone mineral density and physical function in older adults. Osteoporosis International, na . [Journal article] Laird, Eamon, Thurston, Sally, van Wijngaarden, Edwin, Shamlaye, Conrad, Myers, Gary, Davidson, Philip, Watson, Gene, McSorley, E. M., Mulhern, Maria S., Yeates, Alison J., Ward, Mary, McNulty, Helene and Strain, JJ (2017) Maternal vitamin D status and the relationship with neonatal anthropometric and childhood neurodevelopmental outcomes: Results from the Seychelles Child Development Nutrition Study. Nutrients, 9 (11). pp. 1235-1247. [Journal article] Logue, Caomhan, Dowey, Le Roy, Strain, JJ, Verhagen, Hans, Mcclean, Stephen and Gallagher, Alison (2017) Application of Liquid Chromatography−Tandem Mass SpectrometryTo Determine Urinary Concentrations of Five Commonly Used ...
Chebbi, Alif, Elshikh, Mohamed, Haque, Farazul, Ahmed, Syed, Dobbin, Sara, Marchant, R, Sayadi, Sami, Chamkha, Mohamed and Banat, Ibrahim M (2017) Rhamnolipids from Pseudomonas aeruginosa strain W10;as antibiofilm/antibiofouling products for metal protection. Journal of Basic Microbiology, 9999 . pp. 1-12. [Journal article] Elshikh, M, Moya-Ramirez, I, Moens, H, Roelants, S, Soetaert, W, Marchant, Roger and Banat, Ibrahim M (2017) Rhamnolipids and Lactonic Sophorolipids: Natural antimicrobial surfactants for oral hygiene. Journal of Applied Microbiology, 123 . [Journal article] Elshikh, Mohamed, Funston, Scott, chebbi, Alif, Ahmed, Syed, Marchant, R and Banat, Ibrahim M (2017) Rhamnolipids from non-pathogenic Burkholderia thailandensis E264: Physicochemical characterization, antimicrobial and antibiofilm efficacy against oral hygiene related pathogens. New Biotechnology, 36 . pp. 26-36. [Journal article] Funston, SJ, Tsaousi, Konstantina, Smyth, TJ, Twigg, Matthew, Marchant, Roger and Banat, ...
Foot-and-mouth disease virus (FMDV), family Picornaviridae, genus Aphthovirus causes an acute vesicular disease of pigs and wild and domesticated ruminants such as sheep, goats, cattle, water buffalo, and deer. It can cause high death rates in young animals and production losses in adults and is considered to be the single most important constraint to world trade in live animals and animal products. FMDV has a genome consisting of a single strand of positive-sense RNA and seven serotypes of FMDV exist: SAT 1, SAT 2, and SAT 3 are usually restricted to Africa; Asia 1 is restricted to Asia; and O, A, and C are present in Africa, Asia, and South America and occasionally Europe. Globally, FMDV serotypes O and the A are the most prevalent. VetPCR FMDV serotype A Detection Kit is the direct detection of Foot-and-Mouth Disease Virus Serotype A on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain
Adams, M.J., Galvan, S., Reinitz, D., Cole, R.A., Pyare, S., Hahr, M. et Govindarajulu, P. 2007. Incidence of the fungus batrachochytrium dendrobatidis in amphibian populations along the northwest coast of North America. Herpetological Reviews 38:430-431.. Agence canadienne dinspection des aliments. 2009. Les maladies déclarables et les maladies à notification immédiate et annuelle : un guide pour les intervenants du milieu agro-alimentaire et les laboratoires [en ligne]. Agence canadienne dinspection des aliments, Gouvernement du Canada. (consulté le 23 Oct. 2009).. Agence de la santé publique du Canada. 2006. Maladie de Lyme - fiche de renseignements [en ligne]. Gouvernement du Canada. (consulté le 11 janvier 2009).. Alexander, D.J. 2000. Newcastle disease and other avian paramyxoviruses. Revue scientifique et technique (Office international des épizooties) 19:443-462.. Alford, R.A. et Richards, S.J. 1999. Global amphibian declines: a problem in applied ecology. Annual Review of ...
SALLU, Raphael S. et al. Molecular survey for foot-and-mouth disease virus in livestock in Tanzania, 2008-2013. Onderstepoort j. vet. res. [online]. 2014, vol.81, n.2, pp.1-6. ISSN 2219-0635.. Phylogeography data are of paramount importance in studying the molecular epidemiology dynamics of foot-and-mouth disease virus (FMDV). In this study, epithelial samples and oesophageal-pharyngeal fluids were collected from 361 convalescent animals (cattle and buffaloes) in the field throughout Tanzania between 2009 and 2013. The single plex real-time RT-PCR (qRT-PCR) assay for rapid and accurate diagnosis of FMDV employing the Callahan 3DF-2, 3DF-R primers and Callahan 3DP-1 probe were used. Preparation of the samples was performed according to the OIE manual, with a Kenya O serotype obtained from the attenuated vaccine serving as a positive control and samples collected from healthy animals serving as true negatives. The results indicated that 53.49% of samples (n = 176) were positive for FMDV genome by ...
Antibodies are other respectable achievements of our R&D experts, recognized by Vietnam Ministry of Science and Technology. Among that, HANVET K.T.G is a sterile emulsion, containing specific antibodies against infectious bursal disease (IBD), Newcastle disease (ND) and Infectious bronchitis (IB) in chickens; that obtained from hyper-immunized hen egg-yolk. In addition, the preparation acts as a non-specific protein-therapy and increases the bodys resistance to prevent infections and secondary infections. HANVET K.T.G has also been recognized by the first prize of VIFOTEC 2007 for a creative bio-product and the second prize at Korean International Expo in 2007 ...
Although oseltamivir-resistant pandemic influenza A(H1N1)pdm09 is uncommon in immunocompetent individuals, a recent report from Newcastle, Australia, showed the first sustained community spread, from June to August 2011, of oseltamivir-resistant influenza A(H1N1)pdm09 virus carrying the H275Y neuraminidase (NA) mutation. To determine the frequency and the extent of this viral variant spread in the nearest major city to Newcastle, we performed a sequence-based genotypic assessment on samples from 143 oseltamivir untreated and 23 oseltamivir post-treatment individuals with influenza collected contemporaneously in Sydney, 120 km southwest of Newcastle. The detection of two of 143 (1.4%) community-derived samples containing H275Y suggests a low prevalence of oseltamivir-resistant influenza A(H1N1)pdm09 virus in the general community and no convincing evidence of spread of the NA H275Y-bearing influenza A(H1N1)pdm09 virus. In oseltamivir treated patients, oseltamivir-resistant influenza A(H1N1)pdm09 virus
Pigeons are probably the most common nuisance bird. Everyone knows that pigeons have adapted to life in the city, and they seem to be everywhere in urban environments. Unfortunately, the bird lovers of the world feed them, and they have developed a dependence upon people, thus reinforcing their dependency upon urban areas. They roost on signs, ledges, almost anywhere, and they bring nesting material and leave droppings everywhere. Pigeons are swimming with filth and disease, as everyone knows. Pigeons can carry or transmit: encephalitis, histoplasmosis, Newcastle disease, pigeon ornithosis, cryptococcosis, pigeon coccidiosis, toxoplasmosis, pseudo-tuberculosis, and salmonella food poisoning. Pigeons can carry fleas, ticks, mites, and other parasites. ...
Mitochondrial Encephalomyopathies, Mitochondrial Myopathies, Oxidative Phosphorylation Disorders, Respiratory Chain Disorders Patrick F Chinnery, PhD, FRCPath, FRCP, FMedSci Institute of Human Genetics Newcastle University Newcastle upon Tyne, United Kingdom [email protected] Initial Posting: June 8, 2000; Last Update: September 16, 2010. Summary Go to: Disease characteristics. Mitochondrial diseases are a clinically heterogeneous group of disorders that arise…
Funding Research grants from the UK Alzheimers Society, the US Alzheimers Association and philanthropic donations. This work was funded by a grant for the PREVENT-Dementia programme from the UK Alzheimers Society (grant numbers 178 and 264), and the PREVENT-Dementia study is also supported by the US Alzheimers Association (grant number TriBEKa-17-519007) and philanthropic donations. JTOB and LS are supported by the Cambridge NIHR Biomedical Research Centre. MJF is supported by the NIHR Newcastle Biomedical Research Centre awarded to the Newcastle Hospitals NHS Foundation Trust and Newcastle University. LS is also supported by Alzheimers Research UK (ARUK-SRF2017B-1). Participants were recruited at West London Mental Health National Health Service (NHS) Trust (now known as West London NHS Trust) and scanning was carried out at the Clinical Imaging Facility, Imperial College London. ...
Mareks disease virus (MDV) also known as Gallid herpes virus 2 (GaHV-2), was an oncogenic poultry herpes virus, causing lymph proliferative and demyelinating disorder in infected chickens. In the 1970s, herpes virus of turkey (HVT) vaccine, which belonged to serotype 3 vaccines, was mainly used to control the disease. In the mid-1980s, a serotype 2…
Jembrana disease was reported initially in Bali cattle (Bos javanicus) on Bali island in 1964 and the causative agent was subsequently identified as a bovine lentivirus and designated Jembrana disease virus (JDV). This atypical lentivirus causes an acutely pathogenic disease that is associated with clinical signs and pathological lesions attributable to a disease primarily affecting the lymphoid system. Based on the intense proliferation of cells in the parafollicular (T-cell) areas of lymphoid tissue it has been assumed that the cellular tropism of the virus was for T-cells.. An initial investigation of the pathological changes following JDV infection provided morphological evidence that JDV infection occurred not in T-cells but probably in centroblast-like cells containing IgG and presumably of B-cell lineage. The identity of the infected cells was confirmed by double immunofluorescence labelling techniques as being IgG-containing CD79α+ cells, indicating that the virus replicated in mature ...
Mareks disease virus is a herpesvirus of chickens that costs the worldwide poultry industry more than US$1 billion annually. Two generations of Mareks disease vaccines have shown reduced efficacy over the last half century due to evolution of the virus. Understanding where the virus is present may give insight into whether continued reductions in efficacy are likely.
DDC classification: 0556,T Dissertation note: The purpose of the present study was to evaluate effects of chioramphenicol on the inimune response of broiler chickens. The immuno-modulatory effects of chioramphenicol in chicks was evaluated using the following parameters: a) Morphometric effects of chioramphenicol on immune organs such as Li ursa of Fabricius, Tliymus and Spleen. b) Effects of cliloramphenicol on the development of antibody titres against Newcastle virus vaccine. c) Potential of chioramphenicol treated and NDV vaccinated birds to resist virulent NDV challenge. d) Comparison of body weight gains of chioramphenicol a iid treated and untreated birds. The untreated control birds had higher mean body weight than chioramphenicol and cyclophosphamide treated birds. Chioramphenicol treatment adversely affect the weight of bursa of Fabricius , spleen and thymus of birds. The normal dose of chioramphenicol slightly depressed the weight of immune organ than double ...
Dawn, I have recently been purifing GST fusion proteins that a colleague constructed and he had used BL21 as the host strain. I have almost undetectable 29k GST coming down with the GSH sepharose(by coomassie), with strong bands of our fusion protein of interest(I can sonicate my cells in only PBS without any difference in the band pattern). You may want to try that strain. Also, since you are have apparent problems with the thrombin cleavage site, you could sub clone into one of the later pGEX series plasmids that contains the Factor Xa cleavage site. Also, try checking that you are not over sonicating your E. Coli, as this would tend to help liberate a lot of free proteases. Hope it works out. Bill In article ,Pine.SUN.3.91.950515134811.5804A-100000 at pip,, Dawn Gray ,gray at oci.utoronto.ca, wrote: , Hi, , I am having trouble with premature cleavage of my gst-fusion protein. I , am using Pharmacias pGex2Tk vector. I can pick up the complete fusion , protein (the correct size plus the gst) ...
Infectious Brusal Disease Virus Antibody Test Kit [IBD-2P] - Kit includes approximately 640 test samples plus controls, contains 5 microtiter plates. Infectious Bursal Disease (IBD) affects primarily young chickens. The condition is considered economically significant due its ability to induce profound immunosuppression in chickens often resulting in susceptibility to secondary bacterial and viral infections. The symptoms include depression, anorexia, ruffled feathers, and
For many years, ND has been one of the most important infectious pigeon diseases in China. In recent years, a high mortality has been observed in ND-infected pigeons in China. Mortality is from 40% to 80% or 100% in some cases. The full-length genomes of four pigeon paramyxovirus type 1 (PPMV-1) strains, which were isolated from infected pigeons in China in 2012 and 2013, were sequenced and analyzed to determine the phylogenetic characteristics of PPMV-1 circulating in pigeons of China in recent years. Furthermore, cross hemagglutination inhibition and cross virus neutralization assays, as well as animal experiments were conducted to determine the antigenicity and pathogenicity of those viruses. Proteolytic cleavage sites (residues 112-117) of the F proteins were identified as the typical virulence motif, (112)RRQKR↓F(117) for all four PPMV-1 strains investigated. Phylogenetic analysis based on sequences of complete genomes and F gene revealed that the four PPMV-1 isolates and most of recent ...
Mwiine, F. N., Ayebazibwe, C., Olaho-Mukani, W., Alexandersen, Soren, Balinda, S. N., Masembe, C., Okurut, A. R. Ademun, Christensen, L. S., Sørensen, K. J. and Tjørnehøj, K. 2010, Serotype specificity of antibodies against foot-and-mouth disease virus in cattle in selected districts in Uganda, Transboundary and emerging diseases, vol. 57, no. 5, pp. 365-374, doi: 10.1111/j.1865-1682.2010.01157.x. ...
The aim of the study was to analyse the prevalence of single-reactor (SR) pigs in Poland, to attempt an explanation for this phenomenon, and to assess whether the occurrence of SR pigs could create problems for a successful Aujeszkys disease virus (ADV) eradication programme in Poland. A total of 6494 ADV gE antibody positive/doubtful sera were retested by gB ELISA and subsequently by virus neutralisation test (VNT) to confirm the results of the glycoprotein E (gE) ELISA. Amongst the serum samples tested, 5.9% could be classified as being taken from SR pigs, as was shown by gE ELISA positive/doubtful results, which were not confirmed by negative findings in gB ELISA and VNT. It means that the observed SR phenomenon was due to a false positive/doubtful reaction in gE ELISA. This finding was strongly supported by the fact that the serum samples were taken from the animals from herds without any previous or subsequent history of Aujeszkys disease. The low percentage of SR pigs does not seem to ...
Borna disease virus (BDV) is a non-segmented, negative-strand RNA virus that replicates and transcribes its genome in the nucleus of infected cells. It uses the cellular splicing machinery to generate a set of alternatively spliced mRNAs from the 2.8 and 7.1 kb primary transcripts, each harbouring two introns. To determine whether splicing of these transcripts is regulated by viral factors, the extent of splicing was studied in infected cells and COS-7 cells transiently transfected with plasmids encoding the 2.8 kb RNA of BDV. Unspliced RNA was found to be the most abundant RNA species in infected cells, whereas viral transcripts lacking both introns were only found in minute amounts. In sharp contrast, plasmid-derived 2.8 kb RNA was predominantly intron 1-spliced and double-spliced. Co-expression of the BDV proteins P, N and X did not influence splicing of plasmid-expressed 2.8 kb RNA. Furthermore, the splicing pattern did not change when the 2.8 kb RNA was expressed in BDV-infected cells. Based on
This study was conducted to investigate the presence of foot-and-mouth disease virus (FMDV) in different geographic locations of Tanzania. Epithelial tissues and fluids (n = 364) were collected from cattle exhibiting oral and foot vesicular lesions suggestive of FMD and submitted for routine FMD diagnosis. The analysis of these samples collected during the period of 2002 and 2010 was performed by serotype-specific antigen capture ELISA to determine the presence of FMDV. The results of this study indicated that 167 out of 364 (46.1%) of the samples contained FMDV antigen. Of the 167 positive samples, 37 (28.4%) were type O, 7 (4.1%) type A, 45 (21.9%) SAT 1 and 79 (45.6%) SAT 2. Two FMDV serotypes (O and SAT 2) were widely distributed throughout Tanzania whilst SAT 1 and A types were only found in the Eastern zone. Our findings suggest that serotypes A, O, SAT 1 and SAT 2 prevail in Tanzania and are associated with the recent FMD outbreaks. The lack of comprehensive animal movement records and ...
The quantitative role of sheep in the transmission of foot-and-mouth disease virus (FMDV) is not well known. To estimate the role of sheep in the transmission of FMDV, a direct contact transmission experiment with 10 groups of animals each consisting of 2 infected lambs and 1 contact calf was performed. Secretions and excretions (oral swabs, blood, urine, faeces and probang samples) from all animals were tested for the presence of FMDV by virus isolation (VI) and/or RT-PCR. Serum was tested for the presence of antibodies against FMDV. To estimate FMDV transmission, the VI, RT-PCR and serology results were used. The partial reproduction ratio R0p i.e. the average number of new infections caused by one infected sheep introduced into a population of susceptible cattle, was estimated using either data of the whole infection chain of the experimental epidemics (the transient state method) or the final sizes of the experimental epidemics (the final size method). Using the transient state method, R0p was
TY - JOUR. T1 - Replacement of foot-and-mouth disease virus cattle tongue titration by in vitro titration. AU - Dekker, Aldo. AU - van Hemert-Kluitenberg, Froukje. AU - Oosterbaan, Anna H.. AU - Moonen, Kimberly. AU - Mouton, Laure. PY - 2018/10/24. Y1 - 2018/10/24. N2 - Titration of foot-and-mouth disease cattle challenge virus in cattle tongue has been the standard for many years in many countries, although titration in animals has been replaced by in vitro methods for all other applications. The objective of the analysis was the replacement of in vivo titration of cattle challenge virus by in vitro titration. Using data from 32 in vivo titration experiments together with the in vitro titration results of the same samples obtained by plaque count on primary lamb or pig kidney cells, as well as data from the virus isolation control chart used in the laboratory, we show that the reproducibility of the in vitro titration is much higher than that of the in vivo titration. The titer on primary ...
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1 York College, The City University of New York, Jamaica, New York.. 2 MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK.. 3 Genetics of Mitochondrial Disorders, Institute of Human Genetics, Technische Universität München, Munich, Germany.. 4 Genetics of Mitochondrial Disorders, Institute of Human Genetics, Helmholtz Zentrum München, Neuherberg, Germany.. 5 Wellcome Centre for Mitochondrial Research, Institute of Neuroscience, Newcastle University, Newcastle upon Tyne, UK.. 6 Kuwait Medical Genetics Center, Kuwait City, Kuwait.. 7 Genetics Division, Department of Pediatrics, King Abdullah International Medical Research Centre, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.. 8 Department of Neurosciences, Unit of Muscular and Neurodegenerative Disorders, Laboratory of Molecular Medicine, Bambino Gesu Childrens Research Hospital, IRCCS, Rome, Italy.. 9 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, ...
Before starting radiotherapy you will start the hormone treatment. You will be given a prescription for Cyproterone acetate tablets (CPA) and Zoladex or Prostap injection. The CPA tablets are to be taken one week prior to your first hormone injection and for two weeks thereafter.. You must complete the course of CPA and they do not need to be continued by your GP.. Your first hormone injection will usually be given to you by the nurse practitioner in clinic however, it can be arranged for this to be administered by your GP or practice nurse at your GP surgery. It is given in the form of an injection that lasts for four weeks. After this your GP or practice nurse will give your injections once every 12 weeks.. These injections are given to stop the production of testosterone from the testicles. Prostate cancer needs testosterone to grow, so stopping its production will slow down the growth of the cancer cells.. You will need to continue on the injections for three monthsbefore the radiotherapy, ...
Objectives: To follow persistent perforations following spontaneous extrusion of a Ventilation Tube (VT) in a Paediatric tertiary care setting in order to determine which factors are predictive for residual perforation after spontaneous extrusion, to examine the natural history of spontaneous healing of the perforation, and to determine which factors may be predictive for the subsequent healing of a perforation. Materials and methods: A prospective series of 80 perforations in 70 children after spontaneous extrusion of their VTs was followed. The 80 ears in which VT extrusion was complicated by residual perforation were compared with the 50 ears which extruded leaving an intact tympanic membrane. The 50 self-paired ears were compared. The 80 perforations were followed for spontaneous healing. The group which eventually healed was compared with the group which did not heal. The design of VT, number of prior VTs, age, and operative findings at the time of insertion (tympanic membrane condition, ...
Speaker. Dr Phil Haslam, Consultant Interventional and Uroradiologist, Freeman Hospital, Newcastle upon Tyne. Abstract. In this webinar Dr Phil Haslam will cover MRI imaging for prostate cancer. He will give a brief overview of prostate cancer, and what the problems are with the current/old strategies of staging. Phil will discuss what the urologist and oncologist need to know from imaging the prostate gland. He will then discuss mpMRI, what it means and how he performs it.. Phil will also discuss how we locate tumour, how we assess its aggressiveness and how we stage the tumour. This will involve discussion of DWI and dynamic contrast enhanced MRI. There are several pitfalls that the radiologist needs to be aware of when interpreting these types of scan and he will be illustrating these with clinical examples. PI-RADS 2 will be discussed (advantages/disadvantages) along with Phils current method for reporting these types of scan.. Biography. Dr Phil Haslam trained in Newcastle qualifying in ...