OBJECTIVE: To assess the effect of azithromycin mass drug administration regimens on spleen rates in children aged 0-5 years.,br/, METHODS: Clinical assessment of spleen size was carried out during a cluster-randomised trial of azithromycin mass treatment for trachoma elimination in The Gambia. Twenty-four communities received three annual mass treatments with azithromycin, and 24 communities received treatment at baseline only.,br/, RESULTS: At the 30-month follow-up, 3646 children aged 0-5 years had spleen examination and measurement. Palpable splenomegaly was significantly lower in annually treated vs. baseline-only treatment communities and in treated vs. untreated children at 24 months in the annual treatment arm.,br/, CONCLUSION: The results suggest an effect of azithromycin on spleen rates at the individual level and are most plausibly due to the antimalarial effects of azithromycin.,br/, ...
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TY - JOUR. T1 - Derivation of a brain tumor-selective monoclonal antibody from hybridoma between mouse myeloma and rat spleen cells immune to syngeneic glioma. AU - Saya, Hideyuki. AU - Masuko, Takashi. AU - Hashimoto, Yoshiyuki. PY - 1985/1/1. Y1 - 1985/1/1. N2 - Fischer 344 (F344) rats hyperimmunized with syngeneic 9L/R, glioma cells produced antibody selective to glioma cells. Hybridomas prepared from the spleen cells of the immunized rat were cloned, and we obtained a hybridoma clone which produced monoclonal IgM antibody, termed FR77, that showed selectivity to glioma cells. Immunoperoxidase staining of cultured cells revealed that FR77 was reactive with 3 lines of rat glioma cells but not with normal F344 rat fibroblasts. Immuno-histochemical staining of F344 rat tissue sections with biotinylated FR77 demonstrated that FR77 could bind with glioma tissue developed by intracerebral injection of 9L/R, glioma cells but not to normal parts of the brain tissues and other normal tissues tested. ...
Limiting dilution analyses with the Poisson model were performed on normal mouse spleen cells alone or in the presence of added cells or soluble factors. When a constant number of T cells activated to sheep red blood cells were added to cultures of diluted spleen cells the mean frequency of response to sheep red blood cells was 5- to 30-fold greater than that of spleen cells diluted alone. Adding a constant number of B cells did not produce a significant change in the mean frequency of response of diluted spleen cells. Since a responding unit is thought to be composed of T cells and B cells, these results indicate that antigen-specific T cells normally limit the generation of responding units when normal spleen cells are subjected to limiting dilution analysis alone. Conversely, B cells appear to be in relative excess.. Culture medium supernatants from mixed cultures of T cells activated to histocompatibility antigens and target cells bearing the antigens to which the T cells have been activated ...
Spleen disorders are mainly a danger when left unaddressed. Although you can survive having you spleen removed (called a splenectomy), you might not be able to survive the causes of spleen disorders. That is why it is important to make an appointment with your doctor if you feel you may have a spleen condition. The purpose and function of the spleen. The spleen is a bodily organ that rests in the left side of the abdominal cavity, between the 9th to 12th ribs. The spleens central purpose is to get rid of old red blood cells, although it also functions as reservoir for blood in case you suddenly need it due to some sort of trauma.
Mugraby, L; Gery, I; and Sulitzeanu, D, Subpopulations of mouse spleen lymphocytes. Iii. Cellular inter- actions in the response to concanavalin a. (1975). Subject Strain Bibliography 1975. 1761 ...
The relative functional maturity of neonatal mouse spleen T- and B-cell populations was assessed by comparing the ability to respond to the thymic-independent antigen, DNP-Ficoll, or thymic-dependent SRBC by producing antibody in vitro. Although mouse spleen cells responded to DNP-Ficoll at an earlier age than they responded to SRBC or TNP-SRBC, the reason for the lag in the T-dependent response was confounded by the finding of high numbers of suppressor T lymphocytes in the neonatal spleen. Thus, small numbers of neonatal spleen T cells or thymocytes significantly decreased the in vitro antibody response of adult spleen cells. Although B lymphocytes appear to be functionally mature soon after birth, their acitivity may be modulated by an excess of suppressor T cells; e.g., the reconstitution of helper cell function in the neonatal spleen required anti-theta treatment before addition of adult helper cells. Suppressive activity attributable to T cells seems to play a dominant role in determining ...
The Spleen has a wide range of functions, from digestion to muscle tone to sensuality. The Spleen manufactures Qi from our food. The Spleens role is to transform and move foods, liquids, and thoughts in the body (i.e. digestion). For this reason the Spleen is called the source of postnatal energy (acquired Qi). It has a primary role in the lymphatic and immune systems. It keeps our tissues, muscles, and thoughts moist, supple, adaptable. The Spleen governs taste and true nourishment. It regulates bio-rhythms including menstruation. The Spleen stores Yi, consciousness of potentials, the ability to focus the mind sharply and keenly. A healthy spleen requires a stabile home environment. The Spleen harmonizes upper and lower parts of the body. Worry and over-thinking are the greatest causes of Spleen depletion.. The Spleen is the largest lymphatic organ, and plays an important part in the bodys immune system, by helping it to recognize foreign invaders. The spleen is responsible for the removal of ...
Question - Lesions on spleen, abdomen pain. Where can I find more information? . Ask a Doctor about diagnosis, treatment and medication for Flu, Ask a Gastroenterologist, Surgical
Header}} ==Introduction== [[File:Stage_22_image_085.jpg,thumb,300px,Developing Human Spleen ([[Carnegie stage 22,stage 22]])]] The {{spleen}} is located on the left side of the abdomen and has a role initially in blood and then immune system development. The spleens haematopoietic function (blood cell formation) is lost with embryo development and lymphoid precursor cells migrate into the developing organ. Mesoderm within the dorsal mesogastrium form a long strip of cells adjacent to the forming stomach above the developing pancreas. Vascularization of the spleen arises initially by branches from the dorsal aorta. The human spleen arises in week 5 within the dorsal mesentery as proliferating mesenchyme overlying the dorsal pancreatic endoderm. Cells required for its hemopoietic function arise from the yolk sac wall and near dorsal aorta. The spleen generates both red and white cells in the 2nd trimester. ,br> [[:Category:Spleen,Category:Spleen]] ,br> {{Heart Links}} ,br> {{Immune Links}} ,br> ...
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You will be placed under general anaesthesia and be completely asleep. A cannula (hollow tube) is placed into the abdomen by your surgeon and your abdomen will be inflated with carbon dioxide gas to create a space to operate. A laparoscope (a tiny telescope connected to a video camera) is put through one of the cannulas which projects a video picture of the internal organs and spleen on a television monitor. Several cannulas are placed in different locations on your abdomen to allow your surgeon to place instruments inside your belly to work and remove your spleen. A search for accessory (additional) spleens and then removal of these extra spleens will be done since 15% of people have small, extra spleens. After the spleen is cut from all that it is connected to, it is placed inside a special bag. The bag with the spleen inside is pulled up into one of the small, but largest incisions on your abdomen. The spleen is broken up into small pieces (morcelated) within the special bag and completely ...
I dont know about spleen, but I think Ill have to try out that neatloaf recipe. Of course, pig spleen could be another good gluten-free alternative to Special K for all we know, but then it would no longer be vegetarian. Ill just assume pig spleen is a better weather predictor than dinner ingredient and stick with the recipe as it is for now. ...
In this report we describe a transplantation system where embryonic spleens are grafted into adult hosts. This model can be used to analyze the cellular and molecular requirements for the development and organization of splenic microenvironments.Whole embryonic day 15 (ED15) spleens, grafted under the kidney capsule of adult mice, were colonized by host-derived lymphocytes and DC and developed normal splenic architecture. Grafts were also able to form germinal centers in response to T-dependent antigen. Using this system we demonstrated that adult host-derived lymphotoxin (LT) a was sufficient for the development of ED15 LTa/ grafts. Grafting of ED15 LTa/ spleens into RAG/ hosts followed by transfer of LT a/ splenocytes revealed no requirement for lymphocyte-derived LT a in the induction of CCL21 or the development of T-zone stroma. These data suggest that interactions between adult lymphoid-tissue inducer-like cells and embryonic stromal cells initiated T-zone development. Furthermore,adult ...
TNF-α production in vitro by spleen mononuclear cells from IL-12 knockout mice (IL-12-/-) in comparison with cells from their wild-type littermates (IL-12+
1. The intracellular distribution of nitrogen, DPNH cytochrome c reductase, succinic dehydrogenase, and cytochrome c oxidase has been studied in fractions derived by differential centrifugation from rat and guinea pig spleen homogenates.. 2. In the spleens of each species, the nuclear fraction accounted for 40 to 50 per cent of the total nitrogen content of the homogenate, and the mitochondrial, microsome, and supernatant fractions contained about 8, 12, and 30 per cent of the total nitrogen, respectively.. 3. Per mg. of nitrogen, DPNH cytochrome c reductase was concentrated in the mitochondria and microsomes of both rat and guinea pig spleens. Seventy per cent of the total DPNH cytochrome c reductase activity was recovered in these two fractions. The reductase activity associated with the nuclear fraction was lowered markedly by isolating nuclei from rat spleens with the sucrose-CaCl2 layering technique. The lowered activity was accompanied by the recovery of about 90 per cent of the homogenate ...
Spleen cells may develop into insulin-producing pancreatic islet cells in adult animals, a breakthrough finding that could yield a potential cure for
The spleen was felt by the ancient Greeks and Romans to play a significant role in human physiology. Aristotle thought that the spleen was on the left side of the body as a counterweight to the right-sided liver.1 He believed that the spleen was important in drawing off residual humors from the stomach. The close relation of the stomach and spleen and the presence of the short gastric vessels so important in present-day splenic mobilization likely encouraged this belief. The spleen was also felt to hinder a mans running, and Pliny reportedly claimed that professed runners in the race that bee troubled with the splene, have a devise to burne and waste it with a hot yron.2 The exceptional speed of giraffes was felt to be related to the erroneous belief that giraffes were asplenic. Early references to removal of the spleen to increase speed make it apparent that it has long been known that the spleen is not absolutely necessary to sustain life. Paracelsus believed that the spleen could be ...
References for Abcams Mouse spleen tissue lysate - total protein (ab7937). Please let us know if you have used this product in your publication
Facts about swollen spleen symptoms. Often few swollen spleen symptoms appear in people suffering from an enlarged or damaged spleen. A swollen spleen is not a disease, but rather is a symptom caused by other underlying problems or disorders. However, an enlarged or damaged spleen can become a life-threatening problem, so recognizing swollen spleen symptoms and their underlying causes is important. The spleen is a fist sized organ located under the ribcage on the left side of the body. It sits next to the stomach and is an easily injured organ.
Capacity of ICSBP−/− spleen cells to transfer Listeria resistance to RAG2−/− mice. Non adherent spleen cells of ICSBP+/+ (open diamonds) or ICSBP−/−
Dupuy dangeac, A; Reme, T; Pla, M; and Colombani, J, Accessory function of a small radioresistant spleen cell population in the generation of t-cell-mediated cytotoxicity. (1982). Subject Strain Bibliography 1982. 2379 ...
Female mice were injected with a suspension of 0, 2 ml of 10 per cent v/v sheep red blood cells (SRBC) and sacrificed on the 5th day for recording of spleen mass and assay of plaque-forming cells (PFCs) in the spleens. All PFCs from a pool of five spleens and the individual spleen masses were subjected to rigid statistical analysis to verify whether differences from the control groups were significant (P , 0, 05). Three doses of 4 mg cortisol administered at 24-hourly intervals were given at various times relative to the time of immunization to determine the optimum stage at which to inhibit the formation of PFCs. The greatest inhibitory effect was obtained when the first dose was given at -6 h, with a lesser though still significant depression of PFCs with the -30 h dose regimen. Earlier and later injections had relatively little effect. An attempt was made to determine the response to different dosage levels by injecting doubling doses of steroid starting at the -6 h period. A maximal effect ...
DTA-1 mAb abrogates suppression mediated by CD4+ CD25+ T cells. (A) CD4+ CD25- or CD4+ CD25+ T cells (gated as a or b, respectively) were purified by cell-sorter from BALB/c spleen cells. (B) CD4+ CD25+ T cells (open square and closed square) or CD4+ CD25- T cells (open circle and closed circle) purified from 2-month-old BALB/c mice, or these two populations mixed in equal amounts (open triangle and closed triangle), were stimulated for 3 days along with MMC-treated spleen cells as APC in the absence or presence of graded amounts of DTA-1 mAb. Incorporation of [3H] thymidine by proliferating lymphocytes during the last 6 hr of the culture was measured. (C) Spleen cell suspensions prepared from 2-month-old BALB/c mouse were stained with anti-CD4, anti-CD25 and DTA-1. Expression of GITR (DTA-1) on CD4+ CD25+ T cells or CD4+ CD25- T cells is shown in the histogram. The dotted lines represent control staining with an irrelevant Ab.. ...
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According to Knowlton, spleen lacerations or ruptures typically take place from injury (like a car accident or contact sports). These emergency situations cause a break in the spleens surface and can result in severe internal bleeding and signs of shock (fast heart rate, dizziness, pale skin, fatigue), said Knowlton. The Mayo Clinic reported that without emergency situation care, the internal bleeding might end up being deadly.. On the continuum of spleen breakage, a laceration refers to a lower-grade extent of injury, in which just a part of the spleen is harmed. A burst spleen is the highest grade of broken spleen injury, inning accordance with HealthTap, an online network of physicians who respond to health questions.. According to Medical News Today, symptoms of a lacerated or burst spleen consist of pain or tenderness to the touch in the upper left part of the abdomen, left shoulder, and left chest wall, along with confusion and lightheadedness. If you experience any of the symptoms ...
Splenic T cells exposed to concanavalin A (Con A), and subsequently to factors produced by rat spleen cells in response to Con A (Con A sup), acquire the ability to function as helper T (TH) cells in response to xenogeneic erythrocytes (RBC). Help is measured as the reconstitution of the plaque-forming cell response of a spleen cell population depleted of T cells by treatment with anti-Thy-1 serum and complement. We propose that precursor TH cells differentiate during the in vitro treatment into mature TH cells. As differentiation occurs under limiting dilution conditions, an estimation of the precursor frequency should in principle be possible. However, a single-hit Poisson distribution does not fit our data. Instead, we observe, dependent on the T cell concentration, three separate peaks of response. In many experiments, using sheep, horse, and chicken RBC as antigens, we reproducibly find these peaks at 40-190, 600-3,000, and 20,000-100,000 T cells, placed into limiting dilution cultures, ...
You see, after a major battle, there would be hordes of dead bodies all over the place. Lying in the sun like that, the bodies would get all bloated as the gases inside built up, until they would explode, leaving the ground strewn with organs and viscera. It was quite messy, and extremely unhygenic. To deter this, the doctors would wander the battlefield, looking at bodies for signs of puffiness. They would then stab the body in the abdomen, allowing the noxious humors a means of escape. It was obviously known the the reason for the bloating was that dread spirits were taking up residence in the body. Where else would these vile demons go but the spleen? Thus, the term venting the spleen was born. Their scientific principles were not entirely sound ...
You see, after a major battle, there would be hordes of dead bodies all over the place. Lying in the sun like that, the bodies would get all bloated as the gases inside built up, until they would explode, leaving the ground strewn with organs and viscera. It was quite messy, and extremely unhygenic. To deter this, the doctors would wander the battlefield, looking at bodies for signs of puffiness. They would then stab the body in the abdomen, allowing the noxious humors a means of escape. It was obviously known the the reason for the bloating was that dread spirits were taking up residence in the body. Where else would these vile demons go but the spleen? Thus, the term venting the spleen was born. Their scientific principles were not entirely sound ...
BioAssay record AID 196412 submitted by ChEMBL: Percent inhibition of Concanavalin A stimulated cell proliferation of rat spleen lymphocytes at 10 uM; ND is not determined.
Early detection of problems which concern the spleen is highly critical because when these are just being left untreated, these may probably lead to life-threatening situations. The treatment for pain relating to the spleen would vary immensely depending on the underlying pathology. Before your physician would prescribe you a specific treatment, your exact condition must first be determined. For instance, when the splenic pain is primarily felt due to an underlying infection may entirely be treated with antibiotics. When for instance this is caused by the presence of tumor near the spleen, surgery, chemotherapy and other common ways of treating cancer may help palliate or perhaps treat the condition. However, when it is all due to a ruptured spleen, a splenectomy, which is the surgical removal of the spleen, may be indicated. This surgical procedure poses several risks though. Since the spleen has been already removed, the person this time becomes more susceptible to infections. Thus, vaccines ...
Enlarged Spleen is a disease of the Spleen. Spleen is present under the rib cage in the upper left part of the Abdomen. Its normal size is about that of a fist. But as a result of diseases the Spleen gets enlarged. The Spleen is part of the immune system. It filters the blood and acts as a reservoir. It produces lymphocytes, a type of white ...
The immature B cells that successfully make it through this process (only about 10%) enter into the bloodstream and migrate to the spleen. The immature B cells have both IgM and IgG (BCRs) expressed on their surface at this point and once they enter into the spleen are called transitional type 1 (T1) B cells. In the spleen B cells, T cells, and follicular dendritic cells, form what is known as a primary follicle or sometimes the white pulp (See Figure 2). The T1 B cells are located outside of the follicle (extrafollicular) in an area known as the red pulp (as this is where all of the red cells are flowing through the spleen). At this point, the T1 B cells are exposed to more self cells and circulating proteins and if they respond strongly it would indicate autoreactivity and the cells are typically induced to become T3 B cells, which are anergic (which means they become non-responsive to antigen), and will likely die off.. If the T1 B cells survive through this they can then migrate into the ...
I will be having a splenectomy in July and Im very nervous and scared about it. I never have surgery in my life. I am 44 years old female and always keeping up my healthy life style but for some unknown reason. The CT scan shown that I have lesion growing inside my spleen from a spot to 3.1cm in 2 years. I have been having 7 CT, 1 PET/CT and 1 MRI in the past 2 years. After reveiwing the recent CT, my surgeon determined that he will have to make about 3-6 incision in additional of 3 holes for laporascopic procedure so that he can take the whole spleen out through that incision. He gave me 2 options, 1 is to make an incision above the belly or he can make it on the bikini area to hide the scar. My question is, from your surgeon point of view. would it be more difficult to remote the spleen from bikini area than from the above belly button? I just dont want to add a complication by having him removed the spleen from the bikini area. The second question is, what will be the closing method use to ...
Ive been having abdominal spasms, and just got the results from my CT scan--I have a benign cyst on my spleen. The nurse said no treatment is necessary as long as it doesnt get any bigger (which is f...
Jason Witten wondered if his season was over before it ever began when doctors told the Dallas Cowboys star tight end that he had a lacerated spleen.
A joke blog to tickle your spleen (or spleens, since approximately 10% of people have one or more accessory spleens. They may form near the hilum of the main spleen, the junction at which the splenic vessels enter and leave the organ. --Wikipedia). ...
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This method allows you to isolate different subclass mononuclear cells, like B-cells, T cells, CD4+ and CD8+ T, from mouse spleen. By conjugating cells with specific antibodies and subsequently magnetic beads isolation, using the technique from Miltenyi, this allows a high purity.
What you should know about spleen function. Spleen function is probably not the first thing that you think of in the morning when you wake up. The spleen, however, is an important bodily organ. It serves a very important function in terms of keeping your body healthy. The spleen is the warehouse where old blood cells are held until theyre called upon to perform important bodily tasks like healing lacerations.
Looking for spleen? Find out information about spleen. soft, purplish-red organ that lies under the diaphragm on the left side of the abdominal cavity. The spleen acts as a filter against foreign organisms that... Explanation of spleen
The anti-tumor effect of syngeneic spleen cells, xenogeneic immune RNA (l-RNA) and tumor antigen (TA) was studied in a chemically induced (N-methyl-N-nitro-sourethane) colon carcinoma model in BALB/c mice. When the mice were treated, sequentially, with these three elements by local injection under the tumor, complete tumor regression was observed in 25% of the animals. Complete tumor regression was observed in 17% of animals treated by injection of spleen cells pre-incubated with l-RNA in vitro followed by an injection of TA. When spleen cells were treated first with l-RNA and then with mitomycin-C, therapeutic benefit was obtained and the survival rate of mice treated with these spleen cells and TA was significantly higher than that of the control group (no treatment). The anti-tumor effect of this treatment was abrogated completely when spleen cells were treated with mitomycin-C prior to l-RNA incubation. When spleen cells were incubated with TA in vitro or after incubation with l-RNA and then ...
The spleen is the organ located above the stomach and under the ribs, which is responsible for fighting infection and balancing the fluids in the body. Spleen problems range from mild to severe and life-threatening. One of the most common spleen-related problems is splenomegaly, or an enlarged spleen --- may be caused by numerous factors, states MayoClinic.com, including mononucleosis, bacterial infections, malaria, liver cirrhosis, destruction of red blood cells or hemolytic anemia, blood cancers, pressure on your spleen veins and certain metabolic disorders.
Four different combinations of F1 hybrid mice [(C57BL/10 X B10.A)F1, (C57BL/10 X B10.BR)F1, B6D2F1, and AKD2F1] were injected intravenously with spleen cells from parental strains. The T-cell-mediated cytotoxic potential of spleen cells from the injected F1 mice was assessed from 4 to 21 d later by in vitro sensitization with trinitrophenyl-modified parental or syngeneic F1 spleen cells (TNP-self) or with allogeneic spleen cells. The cytotoxic potential of the F1 mice to TNP-self as well as to alloantigens was abolished or severely depressed throughout this period when the respective H-2k,a,d parental spleen cells were injected. In contrast, the cytotoxic potential was unaffected or only marginally reduced when H-2b parental cells were injected. The induction of depressed cytotoxic activity was shown to be a result of a population of parental radiosensitive T lymphocytes. The results should be discussed with respect to (a) the genetic and mechanistic parameters associated with the differential ...
Light microscopy of primate spleen showing white pulp (blue) and red pulp (pink). The spleen is part of the bodys immune system and classified as a lymphoid organ. It filters blood and serves to screen the blood for the presence of foreign antigens that may be a threat to the bodys health. The red pulp is formed like a sieve through which blood vessels pass. The white pulp is formed of lymphoid cells, mainly lymphocytes, which initiate an immune response if harmful agents in the blood are detected. The spleen also removes and destroys worn out red blood cells. Magnification x9 when printed at 10 cm. - Stock Image C024/0095
A joke blog to tickle your spleen (or spleens, since approximately 10% of people have one or more accessory spleens. They may form near the hilum of the main spleen, the junction at which the splenic vessels enter and leave the organ. --Wikipedia). ...
BioAssay record AID 409783 submitted by ChEMBL: Increase in 2-arachidonoylglycerol level in Swiss-Webster mouse spleen at 10 mg/kg, ip after 4 hrs relative to control.
The immunogenesis of the human fetus has been investigated by means of the formation of immunoglobulins in vitro, immunofluorescence, morphological studies, and analysis of the immunoglobulins in the serum. Twenty fetuses which were born alive but died soon after delivery, were studied; their ages ranged from 13 to 31 weeks. The results of the spleen cultures demonstrated the synthesis of IgG and IgM, which starts at about the twentieth week of gestation. In the serum, IgM could be detected at about the same period. The immunofluorescent staining of the spleen tissue showed that medium sized and large lymphoid cells as well as plasma cells, even with Russell bodies, were positive for either IgG or IgM. The peripheral blood was also found to contain a small number of medium sized IgG and IgM-positive cells. Both the spleen and the peripheral blood showed a considerable number of fluorescent small lymphocytes which exclusively contained IgM. The relatively high ratio of IgM to IgG production ...
For a recent practical I had to investigate different cell populations in a mouse spleen cell suspension which present MHC class II molecules. 2 antibodies were used-rabbit IG which is specifically anti-mouse Ig, and mouse Ig which specifically binds MHC class II ...
There is an 80s revival happening in music these days. No, this isnt necessarily news unto self. After all, bands like Metric have been enjoying massive success with a sound as rooted in the modern day as it is in the past.. Ottawa-based band Silvergun & Spleen are set to release their full-length debut Semi Truck next Tuesday (September 11). Owing as much to the 80s as any of their contemporaries twice their age, the bands blend of electro-pop is pretty damn infectious.. Silvergun & Spleen are comprised of siblings Marie-Eve Mallet and Veronique Mallet along with John Lenherr and bassist Chris Page-Manson.. Last week, S&S vocalist-guitarist Marie-Eve took the time to chat with The Musicnerd Chronicles last about the groups new record and why the 80s werent so bad in hindsight.. How do you feel the band has grown since the release of your 2011 EP Through My Skeleton? Mallet: I think some of the most noticeable differences that we see is around the fact that we were more willing to ...