TY - JOUR. T1 - Molecular cloning and characterization of coffee cDNA encoding spermidine synthase. AU - Hatanaka, Tomoko. AU - Sano, Hiroshi. AU - Kusano, Tomonobu. N1 - Funding Information: The authors thank Dr T. Hashimoto of this institute for providing bacterial strain, vector, chemical (dcSAM) with valuable information, and Professor T. Yasuda in Kobe University for providing the white callus and embryogenic callus of C. arabica . We also thank N. Kawai and T. Shouji for their excellent technical assistance, and M. Furuta for critical reading of the manuscript. This work was supported by grants from Research for the Future Program of The Japan Society for the Promotion of Science (JSPS-RFTF96R160012), and Science and Culture of Japan, and Nestle Science Promotion Committee. T. Hatanaka is a recipient of post-doctoral fellowship from JSPS. PY - 1999/1/25. Y1 - 1999/1/25. N2 - A cDNA for spermidine synthase (SPDS), which converts putrescine to spermidine using decarboxylated ...

Dive into the research topics of Binding and inhibition of spermidine synthase from Plasmodium falciparum and implications for in vitro inhibitor testing. Together they form a unique fingerprint. ...

Plant aminopropyltransferases consist of a group of enzymes that transfer aminopropyl groups derived from decarboxylated S-adenosyl-methionine (dcAdoMet or dcSAM) to propylamine acceptors to produce polyamines, ubiquitous metabolites with positive charge at physiological pH. Spermidine synthase (SPDS) uses putrescine as amino acceptor to form spermidine, whereas spermine synthase (SPMS) and thermospermine synthase (TSPMS) use spermidine as acceptor to synthesize the isomers spermine and thermospermine respectively. In previous work it was shown that both SPDS1 and SPDS2 can physically interact with SPMS although no data concerning the subcellular localization was reported. Here we study the subcellular localization of these enzymes and their protein dimer complexes with gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vectors. In addition, we have characterized the molecular weight of the enzyme complexes by gel filtration chromatography with in vitro assembled recombinant ...

Polyamines are small poly-cations essential for all cellular life. The main polyamines present in metazoans are putrescine, spermidine and spermine. Their exact functions are still largely unclear; however, they are involved in a wide variety of processes affecting cell growth, proliferation, apoptosis and aging. Here we identify idefix, a mutation in the zebrafish gene encoding the enzyme spermidine synthase, leading to a severe reduction in spermidine levels as shown by capillary electrophoresis-mass spectrometry. We show that spermidine, but not spermine, is essential for early development, organogenesis and colour pattern formation. Whereas in other vertebrates spermidine deficiency leads to very early embryonic lethality, maternally provided spermidine synthase in zebrafish is sufficient to rescue the early developmental defects. This allows us to uncouple them from events occurring later during colour patterning. Factors involved in the cellular interactions essential for colour ...

Protein target information for Polyamine aminopropyltransferase (Mycobacterium tuberculosis CDC1551). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.

InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.

TY - JOUR. T1 - Assay of Aminopropyltransferases. AU - Pegg, Anthony. PY - 1983/1/1. Y1 - 1983/1/1. UR - http://www.scopus.com/inward/record.url?scp=0020643520&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0020643520&partnerID=8YFLogxK. U2 - 10.1016/S0076-6879(83)94045-4. DO - 10.1016/S0076-6879(83)94045-4. M3 - Article. C2 - 6353156. AN - SCOPUS:0020643520. VL - 94. SP - 260. EP - 265. JO - Methods in Enzymology. JF - Methods in Enzymology. SN - 0076-6879. IS - C. ER - ...

Ten replicates were generated from both radiation-exposed and sham-exposed samples. About 900 protein spots were detected in the 2DE gels using PDQuest 7.2 software. Eight protein spots were differentially expressed in the radiation exposed cells: expression of four proteins was down-regulated and expression of four proteins was up-regulated by the exposure. Three of these eight protein spots were identified using Maldi-ToF mass spectrometry (MS): spermidine synthase (SRM), 78 kDa glucose-regulated protein (GRP78) and proteasome subunit alpha type 1 (PSA1). Expression of GRP78 was further examined using western-blot technique, and this technique did not confirm the results obtained with 2DE. The western-blot technique has not detected any effect of the 1800 MHz GSM radiation on the expression of vimentin and Hsp27, the proteins that were affected by the 900 MHz GSM exposure in the earlier experiment. In general, the number of proteins significantly affected by the 1800 MHz GSM radiation in this ...

Buy discount S-Adenosyl-100 (SAMe) for Small Dogs and Cats, 60 Tablets at the cheapest prices. Dogs and cats S-Adenosyl liver supplement pet medications at the best prices. No coupons needed.

DINK32 12.2 does not correctly initalize the memory controller on-board. , DINK32 12.3 claims to do this correctly, but in some testing locally it , still did not get everything correct. Could you be more specific as to what is missing? , Theres a chance that PPCBoot does get this right, however. But Im no , PPCBoot expert. I dont know about the 74xx support in PPCBoot, but PPCBoot sets up the 107 in a MCP8245 on a Sandpoint X3 well-enough to boot linux. PPCboot does not, however, configure more than one bank on the SO-DIMM socket, and does not (currently) read the SPDs, if only for the lack of proper I2C support (at least for the 824x). YMMV. My question is how have_of got set: arch/ppc/mm/init.c: if (!have_of) FREESEC(openfirmware); Otherwise... On the whole reset/reboot the system thread, Im interested in similar solutions for the 824x, since Im having highly similar problems on reboot. :-) Interestingly, the gorom solution works fine for me, as long as my root filesystem is not ...

With the purpose of enhancing environmental stress tolerance of sweet potato (,i,Ipomoea batatas,/i,, cv. Kokei 14), we transformed this plant with spermidine synthase genes derived from ,i,Cucurbita ficifolia,/i, (,i,FSPD1,/i,). The ,i,FSPD1,/i,-transgenic plants showed twice as high spermidine content as the wild type (WT) counterpart in both leaves and storage roots. One of the most characteristic features of the transgenic plants was the increase in the number of storage roots formed under both non-stress and stressful environments. Salt and drought stresses suppressed storage root growth, but the transgenic plants were less affected producing significantly larger mass of storage roots and starches than WT plants under either stress. The transgenic plants also showed increased tolerance to chilling- and heat-mediated damage to photosynthesis compared to the WT plants. Thus, sweet potato was made more tolerant to environmental stresses through introduction of the ,i,FSPD1,/i, genes. This ...

A widespread increase in SpmS (spermine synthase) activity has been produced in transgenic mice using a construct in which the human SpmS cDNA was placed under the control of a composite CMV-IE (cytomegalovirus immediate early gene) enhancer-chicken β-actin promoter. Four separate founder CAG/SpmS mice were studied. Transgenic expression of SpmS was found in all of the tissues examined, but the relative SpmS activities varied widely according to the founder animal and the tissue studied. Very large increases in SpmS activity were seen in many tissues. SpdS (spermidine synthase) activity was not affected. Although there was a statistically significant decline in spermidine content and increase in spermine, the alterations were small compared with the increase in SpmS activity. These results provide strong support for the concept that the levels of the higher polyamines spermidine and spermine are not determined only by the relative activities of the two aminopropyltransferases. Other factors ...

Myc transcriptionally regulates genes involved in processes such as cell proliferation, metabolism, differentiation, and angiogenesis. MYC expression is deregulated in many types of human cancer; therefore discovering the mechanisms behind MYCs role in tumorigenesis is essential. In this dissertation, I have focused on several Myc target genes, Spermidine synthase (Srm); Lactate dehydrogenase (Ldh); 3-phosphoglycerate dehydrogenase (Phgdh); Serine hydroxymethyltransferase (SHMT) 1 and 2; and Pim-3 (a member of the Pim family of serine/threonine kinases). These enzymes play a role in various functions: Spermidine synthase (polyamine synthesis); Lactate dehydrogenase (glycolysis); Phgdh and Shmt (serine metabolism); and Pim-3 (cell signaling). In order to elucidate the impact Myc over-expression has on metabolism in tumorigenesis, we use human cell lines, and transgenic mice as well as cell lines and tissues derived from these mice. The impact of inhibition of these target genes on Myc-driven ...

Looking for online definition of SPDS or what SPDS stands for? SPDS is listed in the Worlds largest and most authoritative dictionary database of abbreviations and acronyms

Reaction mass of Chromate(1-), [N-[7-hydroxy-8-[(2-hydroxy-5-nitrophenyl)azo]-1-naphthalenyl]acetamidato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphthalenolato(2-)]-, hydrogen, compd. with N-cyclohexylcyclohexanamine (1:1) and hydrogen bis[1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with dicyclohexylamine (1:1) and hydrogen bis[N-[7-hydroxy-8-[(2-hydroxy-5-nitrophenyl)azo]-1-naphthyl]acetamidato(2-)]chromate(1-) , compound with dicyclohexylamine (1:1) ...

All rights reserved. The published material is being distributed without warranty of any kind, either expressed or implied. Neither ILO nor WHO nor the European Commission shall be responsible for the interpretation and use of the information contained in this material ...

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Polyamines (PA) have a protective role in maintaining growth and development in Scots pine during abiotic stresses. In the present study, a controlled liquid Scots pine embryogenic cell culture was used for studying the responses of PA metabolism related to potassium deficiency. The transcription level regulation of PA metabolism led to the accumulation of putrescine (Put). Arginine decarboxylase (ADC) had an increased expression trend under potassium deficiency, whereas spermidine synthase (SPDS) expression decreased. Generally, free spermidine (Spd) and spermine (Spm)/ thermospermine (t-Spm) contents were kept relatively stable, mostly by the downregulation of polyamine oxidase (PAO) expression. The low potassium contents in the culture medium decreased the potassium content of the cells, which inhibited cell mass growth, but did not affect cell viability. The reduced growth was probably caused by repressed metabolic activity and cell division, whereas there were no signs of H2O2-induced ...

Spermine synthase Proteins available through Novus Biologicals. Browse our Spermine synthase Protein catalog backed by our Guarantee+.

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1-tert-Butyloxycarbonyl-4-(3-aminopropyl)-piperazine chemical properties, What are the chemical properties of 1-tert-Butyloxycarbonyl-4-(3-aminopropyl)-piperazine 373608-48-1, What are the physical properties of 1-tert-Butyloxycarbonyl-4-(3-aminopropyl)-piperazine ect.

this patch is not a single piece of compiled software that runs like a self-contained black box. rather, it is a system comprised of several programs networked together to provide a single service. so, in order to run this patch you will need the following (dont worry, theyre all free):. occam, a lightweight midi client that speaks osc. supercollider, my development environment of choice for realtime audio. something to control with midi. this could be a hardware synth (if you have the appropriate cables), or ableton, logic, ardour, reason, whatever. choose your poison.. once you have these things installed and ready on your box, we can talk about the implementation. my source code is below. to run this code, paste it into a new window in supercollider, select the entire document, and press . note that is not . you should see the little server window on the bottom left boot up, some crap print to the post window, a hideous grey gui pop up, and occam open up. on the top part of my hideous grey ...

RISK ASSESSMENTS 11 5-(2-Aminopropyl)indole (5-IT) ISSN Report on the risk assessment of 5-(2-aminopropyl)indole in the framework of the Council Decision on new psychoactive substances About

The CSV is a delimited data format that has fields/columns separated by the semicolon character and records/rows separated by newlines. Fields that may contain a special character (semicolon, newline, or double quote), are enclosed in double quotes. For an easier handling, the list of Pre-registered substances is available in four parts ordered by EC number. They contain the same information as the following full list.. ...

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The sterile processing department and its central sterile techs ensure the OR is always ready and prepared for surgical procedures. SPDs are also under more pressure than ever to prevent infection. Pathogens are tiny and can hide anywhere. When the goal is to eliminate 99.999% of them, only a careful, detailed approach will do. Our best-in-class products can help you continue to provide the meticulous care needed to reduce the risk of surgical site infections.. Click on the arrows below to learn more about Medline products.. ...

The pluggable surge protectors Type 2 DAC50, are designed to protect AC powerline at the main switchboard of the installation. These SPDs are based on high energy varistor equipped with thermal disconnector and failure indicator, to comply with standards and to provide a maximum protection efficiency, an high impulse current capability and a improved reliability. The surge protectors DAC50 are available in multipolar version and in several voltages to protect single or 3-phase networks ...

B-1008: from Pseudomonas fluorescens No. 101 B-13L; water soluble basic substance containing spermidine moiety; acid hydrolysis yields spermidine

Polyamines are linear polycationic compounds that play a crucial role in the growth and development of higher plants. One triamine (spermidine, SPD) and two tetraamine isomers (spermine, SPM, and thermospermine, TSPM) are obtained by the transfer of the aminopropyl group from decarboxylated S-adenosylmethionine to putrescine and SPD. These reactions are catalyzed by the specialized aminopropyltransferases. In that respect plants are unique eukaryotes that have independently evolved two enzymes, thermospermine synthase (TSPS), encoded by the gene ACAULIS5 , and spermine synthase, that produce TSPM and SPM, respectively. In this work, we structurally characterize the ACAULIS5 gene product, TSPS, from the model legume plant Medicago truncatula ( Mt ). Six crystal structures of Mt TSPS-one without ligands and five in complexes with either reaction substrate (SPD), reaction product (TSPM), or one of three cofactor analogues (5-methylthioadenosine, S-adenosylthiopropylamine, and adenosine)-give ...

N-tert-Butoxycarbonyl-L-glutamic acid 5-methyl ester dicyclohexylamine salt (CAS 14406-17-8) Market Research Report 2018 aims at providing comprehensive

Golden Time Chemical(Jiangsu) Co., Ltd. is a large enterprise wholly owned by Hong Kong businessmen, which is engaged in the manufacture, import & export of petroleum-chemicals and food additives. It is the worlds largest manufacturer of sodium cyclamate and Chinas biggest producer of cyclohexylamine.

Reprogramming glycolysis for directing glycolytic metabolites to a specific metabolic pathway is expected to be useful for increasing microbial production of certain metabolites, such as amino acids, lipids or considerable secondary metabolites. In this report, a strategy of increasing glycolysis by altering the metabolism of inositol pyrophosphates (IPs) for improving the production of S-adenosyl-l-methionine (SAM) for diverse pharmaceutical applications in yeast is presented. The genes associated with the metabolism of IPs, arg82, ipk1 and kcs1, were deleted, respectively, in the yeast strain Saccharomyces cerevisiae CGMCC 2842. It was observed that the deletions of kcs1 and arg82 increased SAM by 83.3 % and 31.8 %, respectively, compared to that of the control. In addition to the improved transcription levels of various glycolytic genes and activities of the relative enzymes, the levels of glycolytic intermediates and ATP were also enhanced. To further confirm the feasibility, the kcs1 was deleted in

Locate Surge Protective Devices (SPDs) suppliers, manufacturers & distributors in Davenport, Iowa. Interactive map of Davenport, Iowa provided.

InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.

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ERF subfamily transcription factors play an important role in plant abiotic and biotic stress tolerance. A cold responsive ethylene responsive factor (ERF), MfERF1, was isolated from Medicago falcata, an important forage legume that has great cold tolerance. Overexpression of MfERF1 resulted in an increased tolerance to freezing and chilling in transgenic tobacco plants, while down-regulation of the ortholog of MfERF1 in M. truncatula resulted in reduced freezing tolerance in RNAi plants. Higher transcript levels of some stress responsive genes (CHN50, OSM, ERD10C, and SAMS) and those involved in spermidine (Spd) and spermine (Spm) synthesis (SAMDC1, SAMDC2, SPDS1, SPDS2, and SPMS) and catabolism (PAO) were observed in transgenic plants than in WT ...

Buy high quality N,N-Bis(2-chloroethyl)phosphorodiamidic Acid Cyclohexylamine Salt 1566-15-0 from toronto research chemicals Inc.

This information profile on cyclohexylamine (108918) is part of a group of 46 such profiles that provide information about chemicals or industrial processes considered to be potential occupational hazards. Each profile contains summary data on known and suspected health effects, the extent of worker exposure and the industrial importance of either a single chemical, class of chemicals, or a partic

Just bought my dream bike and dont think I can bring myself to fit SPD pedals! I have used SPDs for all my bikes for a few years now for ease of swapping one to the other even for my road bikes. This means only one pair of cycling shoes required.. Now I have a £3k road bike and think I need proper road pedals but which ones?. Are there any that are interchangeable with SPDs and if required what new shoes to buy?. Posted 3 years ago ...

Spermidine is a natural polyamine found in many foods you likely eat; mushrooms, broccoli, apples, nuts, soy, aged cheese & much more.

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Type 2 DACF25 SPDs are used mainly for primary surge protection of single and 3-phase AC networks at the main switchboard. In addition to their thermal disconnector, the range is equipped with a internal fuse against short-circuit currents, which avoidsthe use of a external fuse or circuit-breaker as requested by standard. The operation of one of the both disconnectors will activate the indicator and the remote signalling (option). This compact solution simplifies the implementation and minimum space. The DACF25 surge protectors are designed to be used in a multipolar configuration to protect single-phase, 3-phase ...

Type 2 DACF25 SPDs are used mainly for primary surge protection of single and 3-phase AC networks at the main switchboard. In addition to their thermal disconnector, the range is equipped with a internal fuse against short-circuit currents, which avoidsthe use of a external fuse or circuit-breaker as requested by standard. The operation of one of the both disconnectors will activate the indicator and the remote signalling (option). This compact solution simplifies the implementation and minimum space. The DACF25 surge protectors are designed to be used in a multipolar configuration to protect single-phase, 3-phase ...

TY - JOUR. T1 - Spermine synthase deficiency resulting in X-linked intellectual disability (Snyder-Robinson syndrome).. AU - Schwartz, Charles E.. AU - Wang, Xaiojing. AU - Stevenson, Roger E.. AU - Pegg, Anthony. PY - 2011/5/25. Y1 - 2011/5/25. N2 - Polyamines, small positively charged molecules, are vital for cell proliferation and differentiation. They are found ubiquitously in eukaryotic cells. Additionally, they interact with a wide range of other molecules and some membrane associated receptors. Polyamines, spermidine and spermine, are synthesized by two aminopropyltransferases, spermidine synthase and spermine synthase. Recently, mutations in the latter enzyme have been shown to be responsible for an X-linked intellectual disability condition known as Snyder-Robinson syndrome. Spermine synthase deficiency is thus far the only known polyamine deficiency syndrome in humans.. AB - Polyamines, small positively charged molecules, are vital for cell proliferation and differentiation. They are ...

Salinity-alkalinity stress is known to adversely affect a variety of processes in plants, thus inhibiting growth and decreasing crop yield. Polyamines protect plants against a variety of environmental stresses. However, whether exogenous spermidine increases the tolerance of tomato seedlings via effects on chloroplast antioxidant enzymes and chlorophyll metabolism is unknown. In this study, we examined the effect of exogenous spermidine on chlorophyll synthesis and degradation pathway intermediates and related enzyme activities, as well as chloroplast ultrastructure, gene expression, and antioxidants in salinity-alkalinity-stressed tomato seedlings. Salinity-alkalinity stress disrupted chlorophyll metabolism and hindered uroorphyrinogen III conversion to protoporphyrin IX. These effects were more pronounced in seedlings of cultivar Zhongza No. 9 than cultivar Jinpengchaoguan. Under salinity-alkalinity stress, exogenous spermidine alleviated decreases in the contents of total chlorophyll and chlorophyll

Locate Surge Protective Devices (SPDs) suppliers, manufacturers & distributors in Indiana. Interactive map of Indiana provided.

1 mCi quantities of [Terminal Methylenes-3H(N)]-Spermidine Trihydrochloride are available for your research. Application of [3H]Spermidine can be found in: possible novel mechanism underlying temperature-dependent uptake in nuclear fractions in (murine) brain research, endogenous indoles as novel polyamine site ligands at the NMDA receptor complex in brain research, lysine-spermine conjugate inhibiting polyamine transport and cell growth when given with DFMO in experimental cell research, etc.. ...

Aminoethylcysteine ketimine decarboxylated dimer is a natural sulfur-containing compound detected in human plasma and urine, in mammalian brain and in many common edible vegetables. Over the past decade many studies have been undertaken to identify its metabolic role. Attention has been focused on its antioxidant properties and on its reactivity against oxygen and nitrogen reactive species. These properties have been studied in different model systems starting from plasma lipoproteins to specific cellular lines. All these studies report that aminoethylcysteine ketimine decarboxylated dimer is able to interact both with reactive oxygen and nitrogen species (hydrogen peroxide, superoxide anion, hydroxyl radical, peroxynitrite and its derivatives). Its antioxidant activity is similar to that of Vitamin E while higher than other hydrophilic antioxidants, such as trolox and N-acetylcysteine.

SAT1 - SAT1 (untagged)-Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1 available for purchase from OriGene - Your Gene Company.

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The objective of this project was to synthesize a protected spermidine derivative, which is a polyamine derivative. The spermidine that was to be synthesised is a N8 protected spermidine derivative.

This website uses cookies to improve your experience while you navigate through the website. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may affect your browsing experience ...

The public activities coordination tool (PACT) provides an overview of the substance-specific activities that authorities are working on under REACH and the CLP Regulation. These activities are being carried out in line with ECHAs Integrated Regulatory Strategy.. PACT provides up-to-date information on the activities planned, ongoing or completed by ECHA and/or MSCAs for a given substance in the following areas:. ...

3,5-diamino-N-(3-aminopropyl)-6-chloropyrazine-2-carboxamide: may be useful for thymine-related single nucleotide polymorphism detection; structure in first source

13952-84-6 sec-Butylamine testing. Laboratory testing for CAS number 13952-84-6. sec-Butylamine;Butafume;Propylamine, 1-Methyl-;Tutane;1-Methylpropanamine;1-Methylpropylamine;2-Aminobutane;2-AB;2-Butylamine;sec-C4H9NH2;sec-Butanamine;Deccotane;Frucote;2-Aminobutane base;(Rs)-2-aminobutane;(Rs)-sec-butylamine;Secondary butylamine;CSC 2-Aminobutane. This chemical is colourless liquid with an amine odour

Aldrich-440302; [3-(2-Aminoethylamino)propyl]trimethoxysilane technical grade, |=80%; CAS No.: 1760-24-3; Synonyms: Dow Corning(R) product Z-6020; N-(2-Aminoethyl)-3-(trimethoxysilyl)propylamine; Linear Formula: (CH3O)3Si(CH2)3NHCH2CH2NH2; Empirical Formula: C8H22N2O3Si; available at Sigma-Aldrich.

As if you need another reason to eat more cheese, new research suggests that certain types of this rich dairy staple may be able to promote heart health and even extend your lifespan. A new study published in the journal Nature Medicine revealed that spermidine, a compound found in many naturally occurring substances such as certain…. .repubhubembed{display:none;}. ...