Mammalian spermatogenesis consists of many cell types and biological processes and serves as an excellent model for studying gene regulation at transcriptional and post-transcriptional levels. identified five major regulatory mechanisms termed transcript only, transcript degradation, translation repression, translation de-repression, and protein degradation based on changes in protein level relative to changes in mRNA level at the mitosis/meiosis transition and the meiosis/post-meiotic development transition. We found that post-transcriptional regulatory mechanisms are related to the generation of piRNAs and antisense transcripts. Our results provide a valuable inventory of proteins produced during mouse spermatogenesis and contribute to elucidating the mechanisms of the post-transcriptional regulation of gene expression in mammalian spermatogenesis. Spermatogenesis in animals is usually a complex yet tightly regulated developmental process that involves many cell types. Similar to other ...
... many stages in the seminiferous tubules of the testes. attained from entire testes to end up being separated with a water lean. The STA-PUT technique, exhibited right here, uses a linear BSA gradient and basic sedimentation to individual spermatogenic cells centered on size and mass6-9. The STA-PUT technique offers many advantages over the additional two most broadly utilized strategies to individual spermatogenic cell types: FACS and elutriation10-13. The STA-PUT equipment needs just many items of specific glassware put together in a chilly space or huge refrigerator. Therefore, it is usually much less costly than using a cell sorter or an elutriator. The STA-PUT technique produces higher quantities of cells per cell type and testis than can become categorized by FACS in a similar period framework, although the chastity of each cell 158013-43-5 supplier populace is usually not really as high as those ...
Much prior work has shown that C. elegans spermatogenesis requires the proper morphogenesis and function of its MO secretory vesicles. While many mutants that affect the ultrastructure and cellular position of MO secretory vesicles are known (reviewed by LHernault 2006, 2009), little is known about how the affected proteins alter cell physiology. In this article, we show that morphogenesis of MO secretory vesicles is associated with a physiological transition when this compartment becomes acidified. This acidification is apparently synchronous and occurs coincident with spermatid budding from the residual body, which is when each FB-MO matures into a MO secretory vesicle (Figure 1, D and E). This acidification requires the V-ATPase, which is composed of a V0 sector that forms a pore in the membrane and a V1 sector that hydrolyzes ATP to provide the electromotive force for proton transport through V0 (reviewed by, e.g., Toei et al. 2010). spe-5 mutants do not show this MO acidification. In ...
During spermatogenesis, preleptotene and leptotene spermatocytes, residing in the basal compartment of the seminiferous epithelium, must traverse the blood-testis barrier (BTB) to gain entry to the adluminal compartment for further development at late stage VIII and early stage IX of the epithelial cycle. As such, the timely opening and closing of the BTB is crucial to spermatogenesis. A compromise in this process can lead to infertility. Moreover, the BTB is unique in its relative localization in the seminiferous epithelium compared to the tight junctions (TJs) found in other epithelia. Sertoli cell TJs are situated near the basal lamina in the testis, closest to the basement membrane (a modified form of extracellular matrix [ECM]), unlike TJs found in other epithelia, which are found nearest the apical portion of an epithelium, farthest away from ECM. Needless to say, BTB function in the testis is maintained by intricate regulatory mechanisms. In addition to hormones and cytokines, nitric ...
... (Animation) -At what point in life do spermatogonia begin to undergo meiosis? -How many spermatozoa result from a single primary spermatocyte? -What is spermiogenesis? -What are the structural components of a mature spermatozoon? -How long does it takes for a primary spermatocyte to become a mature spermatozoon? -What testicular hormone is required for maintenance of spermatogenesis? -How do chronically high levels of anabolic steroids such as testosterone suppress spermatogenesis ...
The title of my Ph.D. thesis was "Bioinformatic Analysis of Gene Families in Mouse and Human Spermatogenesis". Initially we determined gene expression profiles during the development of spermatogenesis in newborn mice. I wrote a data integration system to incorporate data from differnt sources including Differential Display, DNA array, and in situ hybridization data. We were able to show that the first wave of spermatogenesis was constituted of three major clusters of expression originating from Sertoli cells, Pachytene germ cells, and spermatids - and that all genes expressed could be associated to one or more of these clusters. This made it possible to determine the germ cell composition of the growing testis from total RNA and work is still ongoing to utilize this to located disrupted germ cell populations that may be caused by hormone like agents such as phthalates, parabenes, and pesticides - leading to impaired testicular function ...
What is the difference between Spermatogenesis and Spermiogenesis? Spermiogenesis consists of a differentiation process while spermatogenesis consists of ..
Mammalian spermatogenesis is regulated by coordinated gene expression in a spatiotemporal manner. The spatiotemporal regulation of major sperm proteins plays important roles during normal development of the male gamete, of which the underlying molecular mechanisms are poorly understood. A-kinase anchoring protein 3 (AKAP3) is one of the major components of the fibrous sheath of the sperm tail that is formed during spermiogenesis. In the present study, we analyzed the expression of sperm-specific Akap3 and the potential regulatory factors of its protein synthesis during mouse spermiogenesis. Results showed that the transcription of Akap3 precedes its protein synthesis by about 2 wk. Nascent AKAP3 was found to form protein complex with PKA and RNA binding proteins (RBPs), including PIWIL1, PABPC1, and NONO, as revealed by coimmunoprecipitation and protein mass spectrometry. RNA electrophoretic gel mobility shift assay showed that these RBPs bind sperm-specific mRNAs, of which proteins are synthesized
de Rooij DG. (2017). The nature and dynamics of spermatogonial stem cells. Development , 144, 3022-3030. PMID: 28851723 DOI. Griswold MD. (2016). Spermatogenesis: The Commitment to Meiosis. Physiol. Rev. , 96, 1-17. PMID: 26537427 DOI. Talwar P & Hayatnagarkar S. (2015). Sperm function test. J Hum Reprod Sci , 8, 61-9. PMID: 26157295 DOI. Yoshida S. (2010). Stem cells in mammalian spermatogenesis. Dev. Growth Differ. , 52, 311-7. PMID: 20388168 DOI. Hogarth CA & Griswold MD. (2010). The key role of vitamin A in spermatogenesis. J. Clin. Invest. , 120, 956-62. PMID: 20364093 DOI. Ruwanpura SM, McLachlan RI & Meachem SJ. (2010). Hormonal regulation of male germ cell development. J. Endocrinol. , 205, 117-31. PMID: 20144980 DOI. Hermo L, Pelletier RM, Cyr DG & Smith CE. (2010). Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 1: background to spermatogenesis, spermatogonia, and spermatocytes. Microsc. Res. Tech. , 73, 241-78. PMID: 19941293 DOI. ...
Taking advantage of conditions that allow spermatogenesis in vitro, the timing and sequence of morphological changes leading from the primary spermatocyte to the spermatozoon is described by light and electron microscopy. Together with previous studies, this allows a detailed description of the nuclear, cytoplasmic, and membrane changes occurring during spermatozoan morphogenesis. By comparison with wild type, abnormalities in spermatogenesis leading to aberrant infertile spermatozoa are found in six fertilization-defective (fer) mutants. In fer-1 mutant males, spermatids appear normal, but during spermiogenesis membranous organelles (MO) fail to fuse with the sperm plasma membrane and a short, though motile. pseudopod is formed. In fer-2, fer-3, and fer-4 mutants, spermatids accumulate 48-nm tubules around their nuclei where the centriole and an RNA containing perinuclear halo would normally be. In all three mutants, spermatids still activate to spermatozoa with normal fusion of their MOs, but ...
SirT1 whole body KO mice have been shown to lack pituitary hormones for reproduction, and fertility in females can be rescued by administration of these hormones (Kolthur-Seetharam et al., 2009; McBurney et al., 2003). The present study demonstrates that SirT1 is necessary in male germ cells for normal spermatogenesis. Mice lacking SirT1 in male germ cells displayed decreased sperm counts, and many of their spermatozoa have aberrant morphology and increased DNA lesions. These defects explain why male germ cell SirT1 KO mice sire only ∼15% of the progeny number that are sired by control mice. Our findings indicate that SirT1 plays an additional essential role in male germ cells.. Cells in the mid-to-late stages of meiosis I contain the highest levels of SirT1, whereas it is present at moderate levels in differentiating spermatogonia and haploid round spermatids. Without SirT1, the appearance of pachytene cells in seminiferous tubules is delayed, demonstrating that SirT1 is required for ...
Spermatogenesis Definition - Spermatogenesis is the process of sperm development. Sperm, the male sex cells, are essential for reproduction, and any...
The spe-10 gene encodes a novel, predicted four-pass integral membrane protein that contains a highly conserved DHHC-CRD motif (Bohm et al. 1997; Putilina et al. 1999). If a potential glycosylation site following TM4 is utilized, then the N-terminal region, the DHHC-CRD zinc-finger, and the C-terminal region should all face the lumen of the MO (Figure 6A). This orientation would allow the N-linked glycans to face the exterior of the cell surface when the MOs fuse to the plasma membrane. Northern hybridizations comparing oogenesis-specific and spermatogenesis-specific transcripts indicate that the spe-10 mRNA is found only in worms that are actively engaged in spermatogenesis (Figure 4B). SPE-10 localizes within the lysosome-like FB-MOs and segregates to spermatids as they bud from the residual body during C. elegans spermatogenesis (Figure 8). These results suggest that a lack of wild-type SPE-10 in the FB-MOs of spe-10 mutants probably causes the previously described sperm ultrastructural ...
Methods of assessing spermatogenesis disorders are described. The biological basis of male infertility or sub fertility is not well understood, due to the lack of research on this topic and the primitive state of current diagnostic procedures. For most males afflicted with spermatogenesis disorders, there is no therapy or rehabilitative method. A careful and expert semen evaluation provides import
Generally, age-related testicular changes are associated with an increase of germ cell degeneration, decline of spermatogenesis and androgen decline resulting in a gradual decrease of sperm count. Unfortunately, an association of these findings to vascular atherosclerotic alterations has never been investigated systematically, although arterial lesions in testicular biopsies of azoospermic men have been described already 30 years ago.
The similarity of the Cbx3hypo/hypospermatogenesis defect to Dnmt3L and Miwi2 mutants [21, 22] prompted us to investigate whether there were any changes in the expression of retrotransposon expression in the mutant testes. For this, we used a polyclonal antibody to the L1-encoded ORF1 protein [23]. ORF1p is required for L1 transposition, and its levels of expression are increased in germ cells, as the L1 transposons become de-repressed [24, 25]. Using this antibody, we found that 45% of the tubules in Cbx3hypo/hypotestes that contained germ cells were positive by immunohistochemistry for ORF1 protein expression, compared with 5% in wild-type testes (see Additional file 6 and 7, Figure s6 and Figure s7). Again, this indicates that the Cbx3hypo/hypomutation may affect the same silencing pathway that is affected in the Dnmt3L and Miwi2 mutants [21, 22].. We next investigated whether the Cbx3 hypo mutation affects the expression of the other two HP1 isotypes, HP1α and HP1β. Accordingly, we stained ...
Meiotic sex chromosome inactivation (MSCI) during spermatogenesis is characterized by transcriptional silencing of genes on both the X and Y chromosomes in mid...
Recent studies have shown that TDRDs are critical regulators of spermatogenesis in mice (Pan et al., 2005; Chuma et al., 2006; Shoji et al., 2009; Vasileva et al., 2009). Based on their expression and the spermatogenic stages by which the phenotypes manifest in mutant animals, TDRDs can be classified into two groups: those that show expression from embryonic germ cells and are critical for progression of the meiotic prophase, and those that begin their expression in neonatal germ cells, show prominent expression from the pachytene stage onwards, and are essential for spermiogenesis. The first group includes TDRD1 and TDRD9 (Chuma et al., 2006; Shoji et al., 2009) and the second group includes TDRD4/RNF17 and TDRD6 (Pan et al., 2005; Vasileva et al., 2009). A key phenotype of the first group of mutants is the derepression of retrotransposons, especially the LINE-1 elements, and the consequent meiotic catastrophe (Chuma et al., 2006; Reuter et al., 2009; Shoji et al., 2009), whereas the second ...
Spermatogenesis, the sperm-generating process, is a complex process involving mitosis of spermatogonia, meiosis of spermatocytes and spermiogenesis of spermatids. The protein expression levels have been well studied by high-throughput proteomic studies, however, the PTMs including phosphorylation have been less explored. Using advanced mass spectrometry, we successfully identified 17,971 phosphorylation sites of 4131 phosphoproteins from adult mouse testes. Gene ontology annotation reveals that those phosphoproteins mainly function in spermatogenesis, mitosis, transcription, translational regulation and RNA splicing. Substrate and kinase annotation reveals important roles of PLK family kinases in the testicular phosphoproteome. Using small molecule inhibitor of PLKs, we found that PLKs play important functions in the spermatocyte cell line GC2. This mouse testicular phosphoproteome can be a rich resource for the study of mechanisms of spermatogenesis ...
Important stages of spermatogenesis relative to the transcription. Here, we commence with the differentiation of prespermatogonial gonocytes in spermatogonia. S
TY - JOUR. T1 - Stage-dependent enzymatic activities in spermatogenesis of mice with the standard karyotype and of chromosomal variants with impaired fertility. AU - Redi, C. A.. AU - Hilscher, B.. AU - Winking, H.. PY - 1983. Y1 - 1983. UR - http://www.scopus.com/inward/record.url?scp=0021064209&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0021064209&partnerID=8YFLogxK. M3 - Article. C2 - 6139043. AN - SCOPUS:0021064209. VL - 15. SP - 322. EP - 330. JO - Andrologia. JF - Andrologia. SN - 0303-4569. IS - 4. ER - ...
... , Authors: Flavia R Mangone, Ana Carolina Pavanelli, Maria A. Nagai. Published in: Atlas Genet Cytogenet Oncol Haematol.
Oogenesis vs Spermatogenesis Sex can be one of the most pleasurable things a couple can do. Some do it for fun while some do it for procreation. All of the
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... :new: SpermatogenesisMethods and ProtocolsSeries: Methods in Molecular Biology, Vol. 927 Barnard, Lori; Aston, Kenneth I. (Eds.)2013
Spermatogenesis arrest information including symptoms, diagnosis, misdiagnosis, treatment, causes, patient stories, videos, forums, prevention, and prognosis.
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Sperm production. Coloured scanning electron micrograph (SEM) of sperm cells (spermatozoa) in the seminiferous tubules of the testis. This is the site of spermatogenesis (sperm production). Each sperm cell consists of a head (grey), which contains the genetic material that fertilises the female egg cell, and a tail (blue), which propels the sperm. The heads of the sperm are buried in Sertoli cells (pink), which nourish the developing sperm. - Stock Image C016/9765
Sperm production. Coloured scanning electron micrograph (SEM) of sperm cells (spermatozoa) in the seminiferous tubules of the testis. This is the site of spermatogenesis (sperm production). Each sperm cell consists of a head (green orange), which contains the genetic material that fertilises the female egg cell, and a tail (blue), which propels the sperm. The heads of the sperm are buried in Sertoli cells (yellow and orange), which nourish the developing sperm. - Stock Image C003/0692
We have published three new studies related to hybridization and speciation. Our study on the disruption of gene expression during spermatogenesis in mice led by postdoc Erica Larson has been accepted in Molecular Biology and Evolution. We present the most detailed assessment of hybrid gene expression across mouse spermatogenesis to date. Aided by novel FACS cell-specific expression data, we find evidence for disruption of X chromosome regulation at multiple stages of spermatogenesis.. Our study on the disruption of gene expression during placental development in hamsters led by recent PhD graduate Tom Brekke has been published in Evolution. In this work we present evidence for extensive disruption of genomic imprinting associated with placental and embryonic overgrowth in hybrid dwarf hamsters. Finally, a collaborative study examining the dynamics of mitochondrial introgression in chipmunks has been published in Genome Biology and Evolution. This work was led by lab postdoc Brice Sarver as part ...
This study aimed to explore the regulatory mechanism of metabolism of xenobiotics by cytochrome P450 during the differentiation process of chicken embryonic stem cells (ESCs) into spermatogonial stem...
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Dynamic analysis of testicular histology of Meig1-deficient mice during the first wave of spermatogenesis. Representative testicular sections stained with Hemat
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In a screen for RNA binding proteins expressed during murine spermatogenesis, we cloned a novel, ancient zinc finger protein possessing a region common to a small class of RNA binding proteins. Zfr (zinc ...
In a screen for RNA binding proteins expressed during murine spermatogenesis, we cloned a novel, ancient zinc finger protein possessing a region common to a small class of RNA binding proteins. Zfr (zinc ...
Grimaldi P, Orlando P, Di Siena S, Lolicato F, Petrosino S, Bisogno T, Geremia R, De Petrocellis L and Di Marzo V. The endocannabinoid system and pivotal role of CB2 receptor in mouse spermatogenesis. PNAS 2009; 106(27):11131- ...
PMID 19926886] Some Single Nucleotide Polymorphisms of the TSSK2 Gene May Be Associated with Human Spermatogenesis Impairment ...
Kallistem is a biotech company specialized in spermatogenesis and in testicular toxicity in vitro. Originating from the Institute of Functional Genomics of Lyon (ENS Lyon), their research in the past 17 years aimed to develop and validate an innovative technology, Bio-AlteR®. This innovatide mdel is a unic technology for predicitve toxicology dedicated to testicular toxicity.
Gene target information for SPATA7 - spermatogenesis associated 7 (human). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
SPATA16 antibody (spermatogenesis associated 16) for IHC-P, WB. Anti-SPATA16 pAb (GTX109460) is tested in Human samples. 100% Ab-Assurance.
TY - JOUR. T1 - Stem Cell Defects in ATM-Deficient Undifferentiated Spermatogonia through DNA Damage-Induced Cell-Cycle Arrest. AU - Takubo, Keiyo. AU - Ohmura, Masako. AU - Azuma, Masaki. AU - Nagamatsu, Go. AU - Yamada, Wakako. AU - Arai, Fumio. AU - Hirao, Atsushi. AU - Suda, Toshio. PY - 2008/2/7. Y1 - 2008/2/7. N2 - Mammalian spermatogenesis is maintained by stem cell capacity within undifferentiated spermatogonial subpopulation. Here, using a combination of surface markers, we describe a purification method for undifferentiated spermatogonia. Flow cytometric analysis revealed that this population is composed of Plzf-positive cells and exhibits quiescence and the side population phenotype, fulfilling general stem cell criteria. We then applied this method to analyze undifferentiated spermatogonia and stem cell activity of Atm-/- mice. Atm-/- testis shows progressive depletion of undifferentiated spermatogonia accompanied by cell-cycle arrest. In Atm-/- undifferentiated spermatogonia, a ...
Looking for online definition of TPR-containing protein involved in spermatogenesis in the Medical Dictionary? TPR-containing protein involved in spermatogenesis explanation free. What is TPR-containing protein involved in spermatogenesis? Meaning of TPR-containing protein involved in spermatogenesis medical term. What does TPR-containing protein involved in spermatogenesis mean?
A spermatogonial stem cell (SSC) is a subtype of undifferentiated spermatogonium. During foetal development gonocytes develop from primordial germ cells and following this SSCs develop from gonocytes in the testis. SSCs are the early precursor for spermatozoa and are responsible for the continuation of spermatogenesis in adult mammals. The stem cells are capable of dividing into more SSCs which is vital for maintaining the stem cell pool. Alternatively they go on to differentiate into spermatocytes, spermatids and finally spermatozoa. One SSC is the precursor for multiple spermatozoa and therefore SSCs are much less numerous in the testes than cells undergoing spermatogenesis. In Humans Undifferentiated spermatogonia can be split into 2 groups; A Dark (Ad) and A Pale (Ap) Ad spermatogonia are reserve stem cells. These cells are capable of dividing to produce more SSCs but usually do not. Ap spermatogonia are actively dividing to maintain the stem cell pool. B1-B4 spermatogonia encompass the ...