Motility of basal fragments of sea urchin sperm flagella | Journal of Cell Science
Both live and reactivated sea urchin sperm flagella were broken by passage through a pipette. Distal fragments stopped, but basal fragments continued to beat. Intact flagella were about 48 micrometer long; basal fragments were about 4-20 micrometer long. Beat periods of tritonated fragments were 11% shorter than those of unbroken controls, possibly reflecting decreased viscous loading; beat periods of live fragments were 18% shorter than those of unbroken controls, possibly reflecting reduced rates of ATP consumption as well as decreased viscous loading. The undulations of basal fragments were compared with those of the basal regions of unbroken flagella, using the patterns of development of the radii and angles of basal bends. Fragments closely resembled basal regions of unbroken flagella, except that bends tended to open as they approached the distal end of a fragment, so that their radii increased more rapidly than those unbroken flagella. Fragments about 4 micrometer long contained only one ...
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Homozygous mutations in SPEF2 induce multiple morphological abnormalities of the sperm flagella and male infertility | Journal...
Methods and results We conducted genetic analyses using whole-exome sequencing in 50 Han Chinese probands with MMAF. Two homozygous stop-gain variants (c.910C,T (p.Arg304*) and c.3400delA (p.Ile1134Serfs*13)) of the SPEF2 (sperm flagellar 2) gene were identified in two unrelated consanguineous families. Consistently, an Iranian subject from another cohort also carried a homozygous SPEF2 stop-gain variant (c.3240delT (p.Phe1080Leufs*2)). All these variants affected the long SPEF2 transcripts that are expressed in the testis and encode the IFT20 (intraflagellar transport 20) binding domain, important for sperm tail development. Notably, previous animal studies reported spontaneous mutations of SPEF2 causing sperm tail defects in bulls and pigs. Our further functional studies using immunofluorescence assays showed the absence or a remarkably reduced staining of SPEF2 and of the MMAF-associated CFAP69 protein in the spermatozoa from SPEF2-affected subjects. ...
SIU SOM Histology ERG
The mature human spermatozoon is about 60 m long and actively motile. It is divided into head, neck and tail. The head (flattened, about 5 m long and 3 m wide) chiefly consists of the nucleus (greatly condensed chromatin!). The anterior 2/3 of the nucleus is covered by the acrosome, which contains enzymes important in the process of fertilisation. The posterior parts of the nuclear membrane forms the so-called basal plate. The neck is short (about 1 m) and attached to the basal plate. A transversely oriented centriole is located immediately behind the basal plate. The neck also contains nine segmented columns of fibrous material, which continue as the outer dense fibres into the tail. The tail is further divided into a middle piece, a principal piece and an end piece. The axonema (the generic name for the arrangement of microtubules in all cilia) begins in the middle piece. It is surrounded by nine outer dense fibres, which are not found in other cilia. In the middle piece (about 5 m long), the ...
The ins and outs of building the sperm tail
Sperm swim, lung cells sweep mucus away, and the cells in the female Fallopian tube move eggs from the ovary to the uterus. Underlying these phenomena are flagella slender, hair-like structures extending from the surface ...
KAKEN - Research Projects | Analysis of flagellar movement of golden hamster spermatozoa by measuring sliding between outer...
Principal Investigator:ISHIJIMA Sumio, Project Period (FY):1990 - 1991, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:動物発生・生理学
Patente US6332677 - Stable substrate structure for a wide swath nozzle array in a high ... - Google Patentes
An inkjet printhead includes a compact substrate of increased stability and structural integrity to provide a high resolution 600 dot-per-inch nozzle array having a one-half inch swath. A plurality of ink vaporization chambers are respectively aligned with the nozzles in two longitudinal columns, one column extending longitudinally along one edge of the substrate and a second column extending longitudinally along an opposite edge of the substrate, with ink feed channels communicating through an ink passage from an underside of the substrate around both edges of the substrate to the vaporization chambers. The ink feed channels have thereby been eliminated from the central portion of the substrate, and replaced by the ink feed channels at the edges of the substrate.
Double X Science: Pregnancy 101: Fertilization is another way to come together during sex
A sperm cell is described as having a head section and a tail section. The head, which is shaped like a flattened oval, contains most of the cellular components, including DNA. The head also contains an important structure called an acrosome, which is basically a sac containing enzymes that will help the sperm fuse with an egg (more about the acrosome below). The role of the tail portion of sperm is to act as a propeller, allowing these cells to swim. At the top of the tail, near where it meets the head, are a ton of tiny structures called mitochondria. These kidney-shaped components are the powerhouses of all cells, and they generate the energy required for the sperm tail to move the sperm toward its target: the egg. ...
Recent Articles | Diversity, Neuroscience And Genetics & Genomics | The Scientist Magazine®| Page 36
The tails of polygamous deer mice sperm have longer midsections than the sperm tails of monogamous individuals of a similar species, and this correlates with improved swimming and competitive ability.. 0 Comments. ...
Recent Articles | Japan And Genetics & Genomics | The Scientist Magazine®| Page 20
The tails of polygamous deer mice sperm have longer midsections than the sperm tails of monogamous individuals of a similar species, and this correlates with improved swimming and competitive ability.. 0 Comments. ...
Dynein structure and power stroke
Dynein ATPases are microtubule motors that are critical to diverse processes such as vesicle transport and the beating of sperm tails; however, their mechanism of force generation is unknown. Each dynein comprises a head, from which a stalk and a stem emerge. Here we use electron microscopy and imag …
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Sperm structure and motility in the eusocial naked mole-rat, Heterocephalus glaber : a case of degenerative orthogenesis in the...
We have studied sperm structure and motility in a eusocial rodent where reproduction is typically restricted to a single male and behaviourally dominant queen. Males rarely compete for access to the queen during her estrus cycle, suggesting little or no role for sperm competition. Our results revealed an atypical mammalian sperm structure with spermatozoa from breeding, subordinate and disperser males being degenerate and almost completely lacking a mammalian phylogenetic stamp. Sperm structure is characterized by extreme polymorphism with most spermatozoa classified as abnormal. Sperm head shapes include round, oval, elongated, lobed, asymmetrical and amorphous. At the ultrastructural level, the sperm head contains condensed to granular chromatin with large open spaces between the chromatin. Nuclear chromatin seems disorganized since chromatin condensation is irregular and extremely inconsistent. The acrosome forms a cap (ca 35%) over the anterior part of the head. A well defined nuclear fossa and
Direction of force generated by the inner row of dynein arms on flagellar microtubules. | JCB
Our goal was to determine the direction of force generation of the inner dynein arms in flagellar axonemes. We developed an efficient means of extracting the outer row of dynein arms in demembranated sperm tail axonemes, leaving the inner row of dynein arms structurally and functionally intact. Sperm tail axonemes depleted of outer arms beat at half the beat frequency of sperm tails with intact arms over a wide range of ATP concentrations. The isolated, outer arm-depleted axonemes were induced to undergo microtubule sliding in the presence of ATP and trypsin. Electron microscopic analysis of the relative direction of microtubule sliding (see Sale, W. S. and P. Satir, 1977, Proc. Natl. Acad. Sci. USA, 74:2045-2049) revealed that the doublet microtubule with the row of inner dynein arms, doublet N, always moved by sliding toward the proximal end of the axoneme relative to doublet N + 1. Therefore, the inner arms generate force such that doublet N pushes doublet N + 1 tipward. This is the same ...
Template:Spermatozoa Terms - Embryology
spermatozoa tail - Following spermiogenesis, the third region of the spermatozoa that has a head, neck and tail). The tail is also divided into 3 structural regions a middle piece, a principal piece and an end piece. In humans: the middle piece (5 µm long) is formed by axonema and dense fibres surrounded by mitochondria; the principal piece (45 µm long) fibrous sheath interconnected by regularly spaced circumferential hoops; the final end piece (5 µm long) has an axonema surrounded by small amount of cytoplasm and plasma membrane ...
Category:Spermatozoa - Embryology
spermatozoa tail - Following spermiogenesis, the third region of the spermatozoa that has a head, neck and tail). The tail is also divided into 3 structural regions a middle piece, a principal piece and an end piece. In humans: the middle piece (5 µm long) is formed by axonema and dense fibres surrounded by mitochondria; the principal piece (45 µm long) fibrous sheath interconnected by regularly spaced circumferential hoops; the final end piece (5 µm long) has an axonema surrounded by small amount of cytoplasm and plasma membrane ...
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OriGene - Odf3 (NM 027019) Human ORF cDNA Clone
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Energy Conversion in Bull Sperm Flagella | JGP
With the use of a specially developed incubation chamber the rates of motility, respiration, and fructolysis were measured simultaneously on semen samples. By inhibiting the respiration with antimycin A, and/or the fructolysis with 2-deoxyglucose, the rates of each of the two ATP-producing pathways could be reduced independently. In this way the ratio of the amount of free energy produced by respiration and by fructolysis could be varied at will from 1 to 0. In uninhibited preparations approximately 75% of the free energy derives from respiration, and 25% from fructolysis. By the use of the absolute rates of respiration, fructolysis, and motility, the efficiency of the conversion of free energy into hydrodynamic work was calculated. After correction for the decay of the preparation during the experiment, this conversion efficiency was found to be 30-45% lower for free energy from respiration than for free energy from fructolysis. The difference in distribution of the enzymes for fructolysis and ...
Verhandlungen der Deutschen Physikalischen Gesellschaft
Meet the beat: an inter-species study of the 3-dimensional flagellar beat — •An Gong1, Sebastian Rode2, Rene Pascal1, Benjamin Friedrich3, Jens Elgeti2, Stephan Irsen1, Luz Perez4, Gerhard Gompper2, Benjamin Kaupp1, and Luis Alvarez1 — 1Center of Advanced European Studies and Research (caesar), Bonn, Germany — 2Institute of Complex Systems, Jülich, Germany — 3Center for Advancing Electronics, Dresden, Germany — 4Instituto de Ciencia y Tecnología Animal, València, Spain. Sperm capture sensory cues that are processed and translated into a time pattern of three-dimensional (3D) flagellar beat for propulsion and steering to find the egg. However, sperm of different species are confronted with quite different fertilization habitats and, accordingly, differ greatly in behaviors. The 3D flagellar beat pattern underlying behaviors and the molecular mechanism involved are not yet understood ...
Txndc2 - Thioredoxin domain-containing protein 2 - Mus musculus (Mouse) - Txndc2 gene & protein
Probably plays a regulatory role in sperm development. May participate in regulation of fibrous sheath (FS) assembly by supporting the formation of disulfide bonds during sperm tail morphogenesis. May also be required to rectify incorrect disulfide pairing and generate suitable pairs between the FS constituents. Can reduce disulfide bonds in vitro in the presence of NADP and thioredoxin reductase.
Does Gene Duplication Perform As Advertised? | Evolution News
Sidc is found to be composed of four paralogs having itself been duplicated twice over. The 5′ untranslated region (UTR) and part of the promoter sequence of the gene derives from AnnX, whereas the translated part and all 300 base pairs (bp) of the 3′ UTR come from the Cdic gene. A sequence comparison of Sdic2 and Cdic reveals that 522 out of 527 residues (99%) can be aligned without difficulty. Sdic has been observed to be expressed in the testes and incorporated into the sperm tail and this is because it has acquired a testis-specific core element, homologous with those of other promoter sequences, from the 3UTR of AnnX. It is unclear whether the element is a translational enhancer or has some other regulatory role in the AnnX gene such as, for example, the mRNA localization. Either way, the gene would seem to contribute to greater fecundity. ...
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Mouse Monoclonal Antibody to SMCP - BidScientific.com
Supplier: ProMab Technologies Type of Product: Monoclonal Antibody Description: Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. The prot
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Moments Notice (page 5)
Karlheinz Stockhausens monumental series of solo piano pieces, Klavierstücke, are inextricably linked to pianist David Tudor. While Stockhausen began composing the cycle in 1952, before he met Tudor, the middle pieces and particularly Klavierstücke XI were composed or re-worked specifically for him. Stockhausen originally planned to write 21 pieces grouped into six sets. The first set (I - IV) was written between 1952 and 1953, the second (V - VIII and XI) between 1954 and 1956, IX and X were finished in 1961 and then came a long break. He began working on additional cycles in the late 70s finishing eight more, some of which were written for synthesizer and electronic keyboards while also incorporating tape and sound projection.. This release re-issues the first recordings of the cycle made in 1958 and 1959, documenting all of the pieces that had been completed up until then. Around the time of the recording, Tudor was traveling frequently in Europe and the US, performing the post-war ...
Red Blood Cell Amigurumi - DIY Geekery
Amazing what you can get done while procrastinating, isnt it? Thats my justification, anyway. Heres a very simple pattern to crochet an erythrocyte (red blood cell), but if you leave out the middle piece the same pattern could be used for any toroid shape. Like a donut, or . Just sayin. Outer ring: ch 12 sc…
Temperature Matching Puzzles
Students will understand the relationship between Fahrenheit and Celsius using these three-piece Temperature Matching Puzzles!Students read the thermometers printed on the middle pieces and find the matching temperatures.Thirty puzzles are pac
TEKT3 Gene - GeneCards | TEKT3 Protein | TEKT3 Antibody
Complete information for TEKT3 gene (Protein Coding), Tektin 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
CiNii 論文 - What have we learned about sea urchin sperm bindin?
YAMAGUCHI Nobuyuki , HIGA Mikihiko , AIBA Kazuhiro , FANG Hui , TANAKA Yoshimasa , URUSHIHARA Hideko Development, growth & differentiation 38(3), 271-279, 1996-06-01 参考文献26件 被引用文献2件 ...
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OriGene - SPEF2 (NM 144722) cDNA Clone
SPEF2 - SPEF2 (untagged)-Human sperm flagellar 2 (SPEF2), transcript variant 2 available for purchase from OriGene - Your Gene Company.
Forums - ADDING WEIGHT & BODY
I have run into what seems to be somewhat of a common problem however. I am getting some pretty noticeable hum. I only have two sources coming from my Decware input selector box into the amp. One is a Denon turntable and the other is an Oppo 105 BDP. When I remove the interconnects from the Oppo the hum is eliminated. All of my components are run through a low dollar surge protector which is going into a standard wall socket. I have tried switching plugs around. Moving the Oppo/Amp off of the surge protector individually and plugging them directly into the wall with no change in the hum (didnt really expect to make a difference). I dont have the budget to have a special circuit created just for this room or the buy any exotic type of power conditioner. Any suggestions as to a reasonable remedy would be welcomed. I have a Rachel amp and have read in the forum that their may be a difference in the grounding on these compared to other Decware amps. I am no engineer and dont know if this actually ...
Forums - ADDING WEIGHT & BODY
I have run into what seems to be somewhat of a common problem however. I am getting some pretty noticeable hum. I only have two sources coming from my Decware input selector box into the amp. One is a Denon turntable and the other is an Oppo 105 BDP. When I remove the interconnects from the Oppo the hum is eliminated. All of my components are run through a low dollar surge protector which is going into a standard wall socket. I have tried switching plugs around. Moving the Oppo/Amp off of the surge protector individually and plugging them directly into the wall with no change in the hum (didnt really expect to make a difference). I dont have the budget to have a special circuit created just for this room or the buy any exotic type of power conditioner. Any suggestions as to a reasonable remedy would be welcomed. I have a Rachel amp and have read in the forum that their may be a difference in the grounding on these compared to other Decware amps. I am no engineer and dont know if this actually ...
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Official OPPO UDP-203 Owners Thread - Page 730 - AVS Forum | Home Theater Discussions And Reviews
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sito.synergy.hygrid[22284]: Its been a while
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Ayus Piece of Mind..: November 2016
لا يُؤَاخِذُكُمُ اللَّهُ بِاللَّغْوِ فِي أَيْمَانِكُمْ وَلَكِنْ يُؤَاخِذُكُمْ بِمَا عَقَّدْتُمُ الأيْمَانَ فَكَفَّارَتُهُ إِطْعَامُ عَشَرَةِ مَسَاكِينَ مِنْ أَوْسَطِ مَا تُطْعِمُونَ أَهْلِيكُمْ أَوْ كِسْوَتُهُمْ أَوْ تَحْرِيرُ رَقَبَةٍ فَمَنْ لَمْ يَجِدْ فَصِيَامُ ثَلاثَةِ أَيَّامٍ ذَلِكَ كَفَّارَةُ أَيْمَانِكُمْ إِذَا حَلَفْتُمْ وَاحْفَظُوا أَيْمَانَكُمْ كَذَلِكَ يُبَيِّنُ اللَّهُ لَكُمْ آيَاتِهِ لَعَلَّكُمْ تَشْكُرُونَ (٨٩) ...