Mira NP, Henriques SF, Keller G, Teixeira MC, Matos RG, Arraiano CM, Winge DR, S -Correia I. Identification of a DNA-binding site for the transcription factor Haa1, required for Saccharomyces cerevisiae response to acetic acid stress. Nucleic Acids Res. 2011 May 17. [Epub ahead of print ...
Only the PBM motif is a classic HLH motif. Three different ChIP-chip-derived motifs are all diverse, but all score highly on ChIP-chip data! Are they motifs of other TFs? Check. 602: GCN4; 1095, TEC1; 1096: resembles 602, but is a closer match to CUP9/TOS8. Also hits GCN4. According to the literature (PMID: 9032238) the core binding site for the Rtg1p-Rtg3p heterodimer is 5-GGTCAC-3; the only motif that resembles this is 1446. Vague resemblance to 602 and 1096. I am going to retain 1446, which represents the literature site; PBM motif 870, which resembles an E-box, and ChIP-chip motif 1445, which scores highest on ChIP-chip data. But give all low confidence ...
I investigate molecular mechanisms that are at the basis of Hox transcription factor specificity by implementing chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) in Drosophila.
SP100兔多克隆抗体(ab43151)可与人样本反应并经WB, IHC, ChIP实验严格验证,被1篇文献引用并得到3个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
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This gene encodes a member of the kinesin superfamily of microtubule-associated molecular motors with functions related to the microtubule cytosekelton. Members of this superfamily play important roles in intracellular transport and cell division. A similar protein in mouse functions in the beta cell antioxidant signaling cascade, acting as a scaffold for the transcription factor specificity protein 1 (Sp1). Mice that lack this gene exhibit beta cell oxidative stress resulting in hypoinsulinemic glucose intolerance. [provided by RefSeq, Jul 2016 ...
In article ,genecutl-2110960149330001 at kosh-161b-1.berkeley.edu,, genecutl at mendel.berkeley.edu (gc) wrote: ,In article ,5480ah$pk3 at sun168.rz.ruhr-uni-bochum.de,, ,please at use.adress.in.signature (User) wrote: , ,* Hello ! ,* Is there anyone who has experience in ,* calcium-phosphat-coprecipitation of Drosophila Schneider cells S2 ? ,* The problem is, that I cannot detect any RNA of my transfected DNAs ,* by Nuclease S1 - Analysis: For example : VAI is a high expressed ,* RNA Pol III - Gen, but the RNA is undetectable after RNA-Preparation ,* by the GIT-method. ,* Have you got any tips for a solution. ,* ,* Thanks for your time ! ,* ,* Björn Sandrock ,* AG Benecke ,* Ruhr-Uni-Bochum, Germany , ,Do your cells look OK after transfection? When you do the precipitation, ,what does the DNA mix look like? Have tried other assays, such as ,luciferase or beta-gal to double check? , ,--gc Hi again ! I think, that my problem might be a problem of the number of cells, which I have used the ...
TY - JOUR. T1 - Pin1-mediated Sp1 phosphorylation by CDK1 increases Sp1 stability and decreases its DNA-binding activity during mitosis. AU - Yang, Hang Che. AU - Chuang, Jian Ying. AU - Jeng, Wen Yih. AU - Liu, Chia I.. AU - Wang, Andrew H.J.. AU - Lu, Pei Jung. AU - Chang, Wen Chang. AU - Hung, Jan Jong. PY - 2014/12/16. Y1 - 2014/12/16. N2 - We have shown that Sp1 phosphorylation at Thr739 decreases its DNA-binding activity. In this study, we found that phosphorylation of Sp1 at Thr739 alone is necessary, but not sufficient for the inhibition of its DNA-binding activity during mitosis. We demonstrated that Pin1 could be recruited to the Thr739(p)-Pro motif of Sp1 to modulate the interaction between phospho-Sp1 and CDK1, thereby facilitating CDK1-mediated phosphorylation of Sp1 at Ser720, Thr723 and Thr737 during mitosis. Loss of the Cterminal end of Sp1 (amino acids 741-785) significantly increased Sp1 phosphorylation, implying that the C-terminus inhibits CDK1-mediated Sp1 phosphorylation. ...
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Mouse monoclonal antibody raised against a partial recombinant SP110. SP110 (NP_004501, 271 a.a. ~ 380 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. (H00003431-M03) - Products - Abnova
Rabbit polyclonal SP100 antibody validated for WB, IHC, ChIP and tested in Human. Referenced in 2 publications and 3 independent reviews. Immunogen…
Polyclonal antibody for SP100 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. SP100 information: Molecular Weight: 100417 MW; Subcellular Localization: Nucleus. Nucleus, PML body. Cytoplasm. Differences in the s
You didnt realise you were already in a dream as you were focusing too much on SP. (this is the very reason I keep telling people at this forum to not focus so much on SP). If you werent focusing on SP and the terrifying images people often get with it, you probably would of just been able to get out of bed and have a good LD but due to your focus on SP and then trying to get out of it, that didnt happen ...
You didnt realise you were already in a dream as you were focusing too much on SP. (this is the very reason I keep telling people at this forum to not focus so much on SP). If you werent focusing on SP and the terrifying images people often get with it, you probably would of just been able to get out of bed and have a good LD but due to your focus on SP and then trying to get out of it, that didnt happen ...
Vasculin antibody [N1N3] (GC-rich promoter binding protein 1) for WB. Anti-Vasculin pAb (GTX122517) is tested in Human samples. 100% Ab-Assurance.
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In the present study, we demonstrate a novel mechanism that causes down-regulation of TβRII. This mechanism involves the binding of an Sp1-HDAC1 complex to a specific Sp1 site of the TβRII promoter in PDAC cells. Treatment of BxPC-3 and MIA PaCa-2 cells with a HDAC inhibitor, TSA, strongly activates TβRII promoter and induces TβRII expression. An increase in the association of TβRII promoter chromatin with acetylated histone H4 was observed after TSA treatment. These findings suggest that histone deacetylation plays a role in repression of the TβRII gene in PDAC. Luciferase reporter assays using serial deletion constructs revealed that a major TSA response region is located within the NRE-2 (−100 to −67) region of the TβRII promoter. Analysis using site-specific mutation constructs demonstrated that a specific Sp1 site (Sp1C) and an inverted CCAAT element located at −102 and −83 bp relative to the transcription start site of TβRII promoter are required for TSA-mediated reversal ...
Shop Type-1 restriction enzyme EcoEI specificity protein ELISA Kit, Recombinant Protein and Type-1 restriction enzyme EcoEI specificity protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Hojo, H. et al. Sp7/Osterix Is restricted to bone-forming vertebrates where it acts as a Dlx co-factor in osteoblast specification. Dev. Cell 37, 1-16 (2016).. This study used ChIP-seq and RNA-seq to analyze the role of the transcription factor Sp7/Osterix during bone formation in mice. The authors generated a transgenic mouse line in which a biotin motif and three FLAG epitopes were attached to the C-terminus of the Sp7 protein to enable the binding sites of Sp7 could be identified by ChIP. ThruPLEX DNA-seq kit was used to generate ChIP-seq libraries to identify osteoblast enhancers. The authors concluded that the appearance of Sp7 within the Sp family was likely to have played a key role in the emergence of bone-forming osteoblasts during vertebrate evolution.. Read now » ...
Hojo, H. et al. Sp7/Osterix Is restricted to bone-forming vertebrates where it acts as a Dlx co-factor in osteoblast specification. Dev. Cell 37, 1-16 (2016).. This study used ChIP-seq and RNA-seq to analyze the role of the transcription factor Sp7/Osterix during bone formation in mice. The authors generated a transgenic mouse line in which a biotin motif and three FLAG epitopes were attached to the C-terminus of the Sp7 protein to enable the binding sites of Sp7 could be identified by ChIP. ThruPLEX DNA-seq kit was used to generate ChIP-seq libraries to identify osteoblast enhancers. The authors concluded that the appearance of Sp7 within the Sp family was likely to have played a key role in the emergence of bone-forming osteoblasts during vertebrate evolution.. Read now » ...
The expression of the trans-acting transcription factor Sp1 in mice was defined by a combination of RNA analysis and immunohistochemical localization of the Sp1 protein. Although ubiquitously expressed, there was an unexpected difference of at least 100-fold in the amount of Sp1 message in different cell types. Sp1 protein levels showed corresponding marked differences. Substantial variations in Sp1 expression were also found in some cell types at different stages of development. Sp1 levels appeared to be highest in developing hematopoietic cells, fetal cells, and spermatids, suggesting that an elevated Sp1 level is associated with the differentiation process. These results indicate that Sp1 has a regulatory function in addition to its general role in the transcription of housekeeping genes.
begingroup$ Hi I previously asked this question. I understand the issue about the within model but I hope you agree it is odd that the pglm documentation explicitly includes within as an option. Anyway I got exactly the same result using the random model as follows: family=list(family=ordered,link=logit) res,-pglm(as.factor(SP_RTG)~NGDP_RPCH+PPPPC_log+GGXWDG_NGDP+PCPIPCH ,index=c(Country,Date),effect=individual,data=dat,family=family, model=random) Error in prepareFixed(start=start,activePar=activePar,fixed=fixed) : argument start is missing, with no default $\endgroup$ - David May 26 15 at 4:46 ...
This graph shows the total number of publications written about "Sp Transcription Factors" by people in this website by year, and whether "Sp Transcription Factors" was a major or minor topic of these publications ...
Sp transcription factors Sp1, Sp3 and Sp4 are highly expressed in cancer cells/tumors and Sp1 is a negative prognostic factor for survival of gastric and pancreatic cancer and glioma patients [43-45]. Although Sp1 and other Sp proteins are important for early embryonic and postnatal development in mice, their expression is relatively low in adult tissues and there is evidence that Sp1 expression decreases with age in rodents and humans [46-48]. The functional importance of Sp1, Sp3 and Sp4 in cancer cells has been confirmed by RNA interference (RNAi) showing that knockdown of Sp transcription factors Sp1, Sp3 and Sp4 (singly or combined) decreases cell proliferation, survival, angiogenesis and inflammation [26, 35, 36, 42]. These results are consistent with identification (by RNAi) of several pro-oncogenic Sp-regulated genes important for cell growth (cyclin D1, EGFR, c-Met), survival (bcl-2, survivin), angiogenesis (VEGF and VEGF receptors), and inflammation (p65 subunit of NFκB) [22, 23, 26, ...
Purpose. Damage to the corneal epithelium results in the massive secretion of fibronectin (FN) shortly after injury and induces the expression of its integrin receptor α5β1. The authors reported previously that FN induces α5 expression in human corneal epithelial cells and rabbit corneal epithelial cells by altering the binding of the transcription factor (TF) Sp1 to a regulatory element from the α5 promoter that it is also flanked by binding sites for the TFs NFI and AP-1. Here, they assessed the function of NFI and AP-1 on α5 gene expression and evaluated the contribution of FN to their overall regulatory influence. Methods. TF binding to the α5 promoter was evaluated in vitro by electrophoretic mobility shift assays and in vivo by ligation-mediated PCR or chromatin immunoprecipitation. TFs expression was monitored by Western blot, whereas their influence was assessed by transfection and RNAi analyses. Results. Coexpression of Sp1, NFI, and AP-1 was demonstrated in all cell types, and ...
Title: Regulation of MAO-A and MAO-B Gene Expression. VOLUME: 11 ISSUE: 15. Author(s):J. C. Shih and K. Chen. Affiliation:Department of Cell andNeurobiology, Keck School of Medicine, University of SouthernCalifornia, 1985 Zonal Ave. Los Angeles, California, 90033, USA. Keywords:mao a, mao b, gene regulation, promoter, sp1, protein kinase c, mapkinase, egr-1. Abstract: MAO A and B genes are made of 15 exons with identical exon-intron organization. They are located on X-chromosome organized in opposite direction, tail to tail with 24kb apart. Both promoters are GC-rich and regulated by transcription factor Sp1. However, they have distinctly different features. MAO B gene, but not MAO A gene, has TATA box. MAO B promoter contains two clusters of overlapping Sp1 sites, the CACCC repressor element. Transcription factors Sp1 and Sp4 can activate MAO B promoter activity through the proximal cluster of Sp1 sites and its activation can be repressed by the over-expression of Sp3 and a related family ...
ID FN908228; SV 1; linear; mRNA; STD; HUM; 2214 BP. XX AC FN908228; XX DT 05-DEC-2010 (Rel. 107, Created) DT 30-AUG-2011 (Rel. 109, Last updated, Version 2) XX DE Homo sapiens mRNA for transcription factor Sp1 (SP1 gene), splice variant c XX KW . XX OS Homo sapiens (human) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. XX RN [1] RP 1-2214 RA Infantino V.; RT ; RL Submitted (04-JUN-2010) to the INSDC. RL Infantino V., Universita degli studi di bari, Farmaco-biologico, Via RL orabona 4, 70125 bari, ITALY. XX RN [2] RX DOI; 10.1016/j.bbrc.2011.07.047. RX PUBMED; 21798247. RA Infantino V., Convertini P., Iacobazzi F., Pisano I., Scarcia P., RA Iacobazzi V.; RT "Identification of a novel Sp1 splice variant as a strong transcriptional RT activator"; RL Biochem. Biophys. Res. Commun. 412(1):86-91(2011). XX DR MD5; 16e1a7647f49a1cfd978aeb25301b12f. DR Ensembl-Gn; ENSG00000185591; ...
Königsmann, T.; Turetzek, N.; Pechmann, M.; Prpic, N. M.: Expression and function of the zinc finger transcription factor Sp6-9 in the spider Parasteatoda tepidariorum. Development Genes and Evolution 227 (6), pp. 389 - 400 (2017 ...
Goat polyclonal SP2/0 Host Cell Proteins antibody validated for WB, ELISA and tested in Mouse. Immunogen corresponding to tissue, cells or virus
The theory of self-reproductive molecular systems involves the consequence that translation must have started from a selected distribution of RNA molecules, that comprised GC-rich sequences of a lengt
The anthrax letter attacks after September 11, 2001 demonstrated the ease of using the anthrax spore as a weapon in a bioterrorist attack. Anthrax is a lethal i...
The ATP-binding cassette (ABC) transporter ABCB1, encoded by the multidrug resistance gene MDR1, is expressed on brain microvascular endothelium and several types of epithelium, but not on endothelia outside the CNS. It is an essential component of the blood-brain barrier. The aim of this study was to identify cell-specific controls on the transcription of MDR1 in human brain endothelium. Reporter assays identified a region of 500bp around the transcription start site that was optimally active in brain endothelium. Chromatin immunoprecipitation identified Sp3 and TFIID associated with this region and EMSA (electrophoretic mobility shift assays) confirmed that Sp3 binds preferentially to an Sp-target site (GC-box) on the MDR1 promoter in brain endothelium. This result contrasts with findings in other cell types and with the colon carcinoma line Caco-2, in which Sp1 preferentially associates with the MDR1 promoter. Differences in MDR1 transcriptional control between brain endothelium and Caco-2 ...
Specificity protein 1 gene is mapped on chromosome 12q13.1 that contains 785 amino acids with a molecular weight of 81kDa encodes a transcription factors which upregulates or downregulates in response to physiological and pathological stimuli. It has three transcript variants encoding in different isoforms found in this gene. Specificity protein 1 binds with high affinity to GC rich sequence that regulates expression of an arrangement of genes drawn in various cellular processes such as growth, differentiation, immune response and apoptosis which in turn modulates cell responses to DNA damage implicated in chromatin remodeling. It binds to the PDGFR-alpha G-box promoter that modulates the cell response to DNA damage as well as functions in the recruitment of SMARCA4 on the c-FOS promoter and necessary in the regulation of FE65 gene expression. Specificity protein 1 is extremely regulated by post translational modification like phosphorylation, proteolytic cleavage, sumoylation, acetylation and ...
The RT² qPCR Primer Assay is the most reliable SYBR® Green-based quantitative real-time PCR assay for gene expression analysis. Our experimentally verified design algorithm yields gene-specific qPCR assays characterized by uniform and high PCR efficiencies and standardized amplification conditions. Every RT² Primer Assay is subjected to rigorous experimental verification. Single product amplification of the correct size and high PCR efficiency are guaranteed when using the appropriate RT² qPCR Master Mixes. The uniform PCR amplification efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses. Browse Primer Assays By Gene ...
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Find info concerning Fairmont State medical transcription. Qualifications for nursing programs vary widely. Learn about the various medical specializations available within technical training programs.
বংশাণু সাধারণতঃ প্রোটিন তৈরির মাধ্যমে তাদের প্রকাশ ঘটায়, যেটি কিনা কোষের সবচেয়ে জটিল কাজগূলো সম্পাদনকারী অণু। প্রোটিন হল এমিনো এসিডের চেইন, আর বংশাণুর ডিএনএ ক্রম (আরএনএ অন্তবর্তীর মাধ্যমে) সুনির্দিষ্ট প্রোটিন ক্রম তৈরির জন্যে ব্যবহৃত হয়। এই প্রক্রিয়াটি বংশাণুর ডিএনএ ক্রমের সাথে মিল থাকা আরএনএ অণু তৈরির মাধ্যমে আরম্ভ হয়, যে প্রক্রিয়াটি প্রতিলিপিকরণ (transcription) নামে পরিচিত। ...
Kluge, S., Vangshardt, R., Ladefoged Larn, A., Zirak-Schmidt, D. H., Dahl, C., Kristensen-McLachlan, R. D., Tessing Schneider, M. & Kewes, P.. 01/02/2020 → 31/12/2023. Projekter: Projekt › Forskning ...
We have characterized the 5′ region of the human alpha 1(V) collagen gene (COL5A1). The transcriptional promoter is shown to have a number of features characteristic of the promoters of housekeeping and growth-control-related genes. It lacks obvious TATA and CAAT boxes, has multiple transcription start sites, has a high GC content, lies within a well-defined CpG island and has a number of consensus sites for the potential binding of transcription factor Sp1. This type of promoter structure, while unusual for a collagen gene, is consistent with the broad distribution of expression of COL5A1 and is reminiscent of the promoter structures of the genes encoding type VI collagen, which has a similarly broad distribution of expression. Stepwise deletion of COL5A1 5′ sequences, placed upstream of a heterologous reporter gene, yielded a gradual decrease in promoter activity, indicating that the COL5A1 promoter is composed of an array of cis-acting elements. A minimal promoter region contained ...
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