Research Corridor has published a new research study titled Solid Phase Extraction Column Market - Growth, Share, Opportunities, Competitive Analysis and Forecast, 2017 - 2025. The Solid Phase Extraction Column market report studies current as well as future aspects of the Solid Phase Extraction Column Market based upon factors such as market dynamics, key ongoing trends and segmentation analysis. Apart from the above elements, the Solid Phase Extraction Column Market research report provides a 360-degree view of the Solid Phase Extraction Column industry with geographic segmentation, statistical forecast and the competitive landscape.. Browse the complete report at http://www.researchcorridor.com/solid-phase-extraction-column-market/. Geographically, the Solid Phase Extraction Column Market report comprises dedicated sections centering on the regional market revenue and trends. The Solid Phase Extraction Column market has been segmented on the basis of geographic regions into North America, ...
The present work describes the development of an analytical method, based on automated on-line solid phase extraction followed by ultra high-performance liquid chromatography coupled with tandem mass spectrometry (SPELC- MS/ MS) for the quantification of 37 pharmaceutical residues, covering various therapeutic classes, and some of their main metabolites, in surface and drinking water. A special attention was given to some glucuronide conjuguates and metabolites of active subtances. Multiple Reaction Monitoring (MRM) was chosen and two transitions per compound are monitored (quantification and confirmation transitions). Quantification is performed by standard addition approach to correct matrix effect. The method provides limit of quantification inferior to 20 ng.L-1 for all compounds. The methodology was successfully applied to the analysis of surface water and drinking water of 8 drinking water treatment plant in west of France. The highest drug concentrations in surface water and drinking water were
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The stationary phase comes in the form of a packed syringe-shaped cartridge, a 96 well plate, a 47- or 90-mm flat disk, or a microextraction by packed sorbent (MEPS) device, a SPE method that uses a packed sorbent material in a liquid handling syringe.[4][5] These can be mounted on its specific type of extraction manifold. The manifold allows multiple samples to be processed by holding several SPE media in place and allowing for an equal number of samples to pass through them simultaneously. In a standard cartridge SPE manifold up to 24 cartridges can be mounted in parallel, while a typical disk SPE manifold can accommodate 6 disks. Most SPE manifolds are equipped with a vacuum port, where vacuum can be applied to speed up the extraction process by pulling the liquid sample through the stationary phase. The analytes are collected in sample tubes inside or below the manifold after they pass through the stationary phase. Solid phase extraction cartridges and disks are available with a variety of ...
n automated method for analyzing free non-cholesterol sterols in human serum using online solid phase extraction-liquid chromatography-mass spectrometry is proposed herein. The method allows the determination of three phytosterols (sitosterol, stigmasterol and campesterol) and two cholesterol precursors (desmosterol and lanosterol). The analysis of sterols in human serum is critical in the study of cholesterol-related disorders, such as inherited familial hypercholesterolemias. Special effort was made to isolate the analytes from the serum lipoproteins, their natural conveyance through the bloodstream. The sample treatment consisted of a Bligh-Dyer extraction followed by dilution of the extract. This treatment allowed the sample to be injected into the online system and ensured the correct detection of the analytes, while avoiding the matrix effects commonly related to serum samples. The analytical performance showed linear ranges that covered two orders of magnitude, with correlation coefficients
Wastewater-treatment plants (WWTPs) are regarded as one of the main sources of antibiotics in the environment. In the present study, the concentrations of multiple antibiotics and their metabolites belonging to 5 antibiotic classes were determined in 3 major Finnish WWTPs. An online solid phase extraction-liquid chromatography-tandem mass spectrometry method was used for the extraction and analysis of the compounds. The method was fully validated using real and synthetic wastewaters. Seven antibiotics and 3 metabolites were found in the analyzed samples. Sulfonamides were removed most efficiently, whereas macrolides usually showed negative removal efficiency during the treatment, which means that the concentrations for individual antibiotics determined in the effluent samples were higher than in the influent samples. Sulfadiazine was found at concentrations up to 1018?ng/L, which was the highest concentration of any of the detected antibiotics in influent. In the effluent samples, the highest ...
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High levels of vanadium have been reported in groundwater (, 0.05 - 5300 g L-1 V) from different parts of Argentina, yet no detailed study of vanadium speciation has been performed. A highly selective strong anion exchange - solid phase extraction (SAX-SPE) method was used (in-situ) for vanadium speciation analysis of groundwater samples from La Pampa - LP (General San Martin and Eduardo Castex) and Buenos Aires - BA (San German) provinces in Argentina. In this method both vanadyl (VIV) and vanadate (VV) were trapped by the complexation with disodium ethylenediaminetetraacetic acid on a pre-conditioned SAX cartridge. In the laboratory, vanadium species were separated at different eluent pH levels. VIV was eluted at pH 4 using methanol and tetrabutylammonium hydroxide. VV was eluted at pH 8 using dihydrogen ammonium phosphate. The eluted species were analysed by inductively coupled plasma mass spectrometry (ICP-MS). This method was validated using an inter-analytical method comparison with ...
Solid phase extraction is a form of digital (step-wise) chromatography designed to extract, partition, and/or adsorb one or more components from a liquid phase (sample) onto stationary phase (sorbent or resin).
10.1002/jssc.201200466. Chapter 3 appears to be the equivalent of a post-print article finally published as: Candish, E., Wirth, H.J., Gooley, A. A., Shellie, R. A., Hilder, E.F., 2015, Characterization of large surface area polymer monoliths and their utility for rapid, selective solid phase extraction for improved sample clean up, Journal of Chromatography A, 1410, 9-18, 10.1016/j.chroma.2015.07.065 ...
Optimization Of Solid Phase Extraction By Experimental Design Methodology For Seperation of Sterol And Tocopherol In Sunflower Oil Deodorizer Distillate Using Zeolite As A Novel Sorbent (Sözlü Sunum ...
Data Bridge Market Research has added an exhaustive research study of the Global solid phase extraction market detailing every single market driver and intricately analyzing the business vertical. The research report has abilities to raise as the most significant market worldwide as it has remained playing a remarka...
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A rapid confirmatory method for determining 12 sulfonamide (SAs) antibacterials in whole milk and eggs is presented. This method is based on the matrix solid-phase dispersion technique with hot water as extractant followed by liquid chromatography (LC)-mass spectrometry (MS). The LC-MS instrument was equipped with an electrospray ion source and a single quadrupole. After 4 mL of a milk sample containing the analytes had been deposited on sand (crystobalite), this material was packed into an extraction cell. SAs were extracted by flowing 4 mL of water through the cell heated at 75 degreesC. With some modifications, this procedure was applied also to eggs. After pH adjustment and filtration, 0.5 mL of the final extracts was then injected into the LC column. MS data acquisition was performed in the positive-ion mode and by monitoring at least three ions for each target compound. The in-source collision-induced dissociation process produced confirmatory ions. At the 50 ng/g level, recovery of the ...
A high-throughput bioanalytical method based on automated sample transfer, automated solid phase extraction, and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) analysis, has been developed for the determination of cetirizine, a selective H1-receptor antagonist. Deuterated cetirizine (cetirizine-d8) was synthesized as described and was used as the internal standard. Samples were transferred into 96-well plates using an automated sample handling system. Automated solid phase extraction was carried out using a 96-channel programmable liquid-handling workstation. Solid phase extraction 96-well plate on polymer sorbent (Strata X) was used to extract the analyte. The extracted samples were injected onto a Betasil silica column (50 × 3, 5 μm) using a mobile phase of acetonitrile-water-acetic acid-trifluroacetic acid (93:7:1:0.025, v/v/v/v) at a flow rate of 0.5 ml/min. The chromatographic run time is 2.0 min per injection, with retention time of cetirizine and ...
Solid Phase Extraction (SPE) cartridges provide a fast and efficient sample clean-up and concentration prior to analysis through GC, HPLC, and/or other instrument methods. SPE method concentrates and purifies analytes from solution by absorption onto a disposable solid phase cartridge, followed by elution of the analyte with a solvent appropriate for instrument analysis.
Phenomenex Inc., a global leader in the manufacture of separation science consumables, introduces Strata Melamine, a solid phase extraction (SPE) sorbent specially designed for food testing. Strata Melamine SPE cartridges simultaneously extracts both melamine and cyanuric acid, cutting sample time in half compared to all other methods.
This thesis describes the development of liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for the trace analysis of active pharmaceutical ingredients (APIs) and their metabolites in aqueous environmental matrices. The research was focused on the development of chiral LC-MS/MS methods for the analysis of fluoxetine and metoprolol, as well as their chiral metabolites in environmental water samples. A method was also developed for the achiral compounds, diazepam and nordiazepam.. The LC-MS/MS methods were validated by the use of the isotope-labeled compounds. As these isotope-labeled compounds were not found in the wastewater samples, the validation could be assessed at trace level concentrations in the actual matrices in which the analytes were detected.. The analytes were extracted from the water samples using solid phase extraction methods. Different types of solid phase extraction sorbents were evaluated. Fluoxetine and norfluoxetine were extracted through the use of a mixed ...
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A simple, rapid and sensitive method for determination of trace levels of domoic acid (DA) in seawater was developed, based on a magnetic solid-phase extraction (MSPE) followed by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Five kinds of ferrite magnetic nanospheres (MFe2O4; M=Fe, Co, Ni, Cu and Zn) were prepared and first used as sorbents for MSPE of DA and ...
Solid phase extraction has become a popular tool for analytical method development as a sample preparation technique. Solid phase sorbents can generally extract analytes based on polarity or selectively based on highly specific interactions between the analyte and an immobilized surface molecule. Biomolecular recognition based solid phases utilize antibodies, nucleic acids, and proteins as a secondary affinity layer on a solid phase support for selective analyte extractions and have been used for sample preparation, purification, separations, bioassays, and molecular diagnostics. Polymer supports for solid phase extraction are desirable for their low cost, abundance, chemical inertness, and pH stability. This thesis reports the use of capillary-channel polymer (C-CP) fibers as a solid support modified for selective extraction and on fiber fluorescence detection of nucleic acids and proteins. The polymer fibers are low cost and their unique shape allows them to be easily adapted into a fluidic fiber
D7168 - 16 Standard Test Method for 99Tc in Water by Solid Phase Extraction Disk , disc, disk, liquid scintillation counting, radioactivity, radiochemistry, solid phase extraction, technetium, water,,
Introduction. The common approach in risk assessment of water bodies is to collect a sample of the water, which is then analysed for potential pollutants in the laboratory. This approach, among other advantages, provides manageable control over accuracy and precision of the results. However, information obtained from spot water samples is only about concentration levels at the time of sampling and may fail to account for episodic contamination. This can be addressed by collecting a series of samples over a time period to obtain a more representative picture of water quality, but the cost of analysis is then increased. An alternative and more cost-effective approach is to obtain time-weighted average (TWA) concentrations of pollutants using passive samplers. Passive samplers are now seen as an alternative to conventional spot samples. This is seen by a number of recent reviews on passive samplers (Vrana et al., 2005; Lu et al., 2002; Petty et al., 2000; Stuer-Lauridsen, 2005; Chimuka and ...
Tetramethylenedisulfotetramine (TETS), commonly known as tetramine, is a highly neurotoxic rodenticide (the LD50, or lethal dose for 50 of test subjects, is 0.1 mg/kg). TETS has been used in hundreds of deliberate and accidental food poisoning events in China. In this report, we describe an extraction method for the quantitation of TETS as spiked into various food matrices, including fruit juices, egg, hot dog, chicken nuggets, turkey deli meat, and ground beef. Quantitation by liquid chromatographyion trap mass spectrometry was based upon selected reaction monitoring of mass-to-charge ratio (m/z) 347 268 and consecutive reaction monitoring of m/z 347268175. An 5 mg TETS concentration was spiked into each food sample. The linear range of quantitation for TETS was 0.520 g/mL. Total recoveries (and percent relative standard deviations) for TETS in various food samples are reported.
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analyzed the content with high resolution LC-MS. Chemometric methods revealed 87 compounds that constitute a specific chemical pattern either qualitatively or quantitatively indicating copepod presence. The majority of the compounds were present in both female and male exudates, but nine compounds were mainly or exclusively present in female exudates and hence potential pheromone candidates. Copepodamide G, known to induce defensive responses in phytoplankton, was among the ten compounds of highest relative abundance in both male and female extracts. The presence of copepodamide G shows that the method can be used to capture and analyze chemical signals from living source organisms. We conclude that solid phase ...
Sphingolipid extraction was performed as described [50] with modifications. Briefly, 500 larvae or 350 adult flies (both male and female were equally represented in control and treatment) were snap‐frozen in liquid nitrogen and homogenized by grinding. The homogenate was added to 201 g of methanol/chloroform/water mixture (3:1:1, v/v/v), followed by sonication for 20 min in room temperature. After overnight incubation, the extract was vortexed and centrifuged at 210 g for 10 min. The organic phase was dried by nitrogen stream, and the dried pellet was re‐dissolved in 2 ml of MeOH/water/CHCl3 (96:94:6, v/v) and applied to a Strata C18‐E solid‐phase extraction cartridge (SPE) (Phenomenex Inc., Torrance, CA, US) which was pre‐conditioned with 4 ml water. Two mL of MeOH was then added into the SPE cartridge after sample loading. A 3 ml of MeOH/CHCl3 (1:1, v/v) was added into the SPE cartridge to elute the sphingolipids. The MeOH/CHCl3 eluent was dried by nitrogen stream. The dried extract ...
Sphingolipid extraction was performed as described [50] with modifications. Briefly, 500 larvae or 350 adult flies (both male and female were equally represented in control and treatment) were snap‐frozen in liquid nitrogen and homogenized by grinding. The homogenate was added to 201 g of methanol/chloroform/water mixture (3:1:1, v/v/v), followed by sonication for 20 min in room temperature. After overnight incubation, the extract was vortexed and centrifuged at 210 g for 10 min. The organic phase was dried by nitrogen stream, and the dried pellet was re‐dissolved in 2 ml of MeOH/water/CHCl3 (96:94:6, v/v) and applied to a Strata C18‐E solid‐phase extraction cartridge (SPE) (Phenomenex Inc., Torrance, CA, US) which was pre‐conditioned with 4 ml water. Two mL of MeOH was then added into the SPE cartridge after sample loading. A 3 ml of MeOH/CHCl3 (1:1, v/v) was added into the SPE cartridge to elute the sphingolipids. The MeOH/CHCl3 eluent was dried by nitrogen stream. The dried extract ...
The present invention relates to fluorinated surfaces which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the surface. Generally, the hydrophilic and electropositive characteristics are expressed at the fluorinated surface. Preferred fluorinated surfaces of the present invention include fluorinated Al(OH)3, fluorinated SiO2 and fluorinated Celite. The fluorinated surfaces of the present invention are particularly useful in processes for purification of DNA from other cellular components. In these processes, a suspension of cellular components is placed in contact with the fluorinated surface, the fluorinated surface is washed to remove all cellular components other than DNA which are bound to the surface, and the bound DNA is eluted from the surface. Lower concentrations of chaotrope in the binding buffer are needed to bind DNA to the fluorinated surfaces.
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A variety of proven techniques, such as filtration, dialysis, liquid/liquid extraction and Solid Phase Extraction (SPE), are routinely adopted in todaylj analytical laboratories to resolve the vast array of sample preparation demands. SPE has established itself as one of the most popular and flexible tools within the analytical laboratory; providing effective, efficient sample concentration and purification prior to HPLC, LC/MS, GC or GC/MS and adaptation to automated techniques such as combinatory chemistry. The resultant SPE product range provides unparalleled performances and satisfies the clients demand for Recovery, Reliability and Reproducibility ...
Solid-phase extraction (SPE) is a technique designed for rapid sample preparation and purification prior to chromatographic analysis. You can optimize your SPE protocols by using SiliCycle SiliaPrepX Polymeric SPE Cartridges and Well Plates.
An efficient, rapid, and selective method for sample pretreatment, namely, molecularly imprinted matrix solid-phase dispersion (MI-MSPD) coupled with gas chromatography (GC), was developed for the rapid isolation of four phosphorothioate organophosphorus pesticides (tolclofos-methyl, phoxim, chlorpyrifos, and parathion-methyl) from carrot and yacon samples. New molecularly imprinted polymer nanomicrospheres were synthesized by using typical structural analogue tolclofos-methyl as a dummy template via surface grafting polymerization on nanosilica. Then, these four pesticides in carrot and yacon were extracted and adsorbed using the imprinted nanomicrospheres and further determined by gas chromatography. Under the optimized conditions, a good linearity of four pesticides was obtained in a range of 0.05–17.0 ng·g|sup|−1|/sup| with |i|R|/i| varying from 0.9971 to 0.9996, and the detection limit of the method was 0.012~0.026 ng·g|sup|−1|/sup| in carrot and
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In phase measuring deflectometry, two orthogonal sinusoidal fringe patterns are separately projected on the test surface and the distorted fringes reflected by the surface are recorded, each with a sequential phase shift. Then the two components of the local surface gradients are obtained by triangulation. It usually involves some complicated and time-consuming procedures (fringe projection in the orthogonal directions, accurate phase shifting).To avoid the complex process, a novel phase extraction algorithm with crossed fringes is presented in this paper. It is based on a least-squares iterative process. Both a numerical simulation and a preliminary experiment are conducted to verify the validity and performance of this algorithm. Experimental results obtained by our method are shown, and comparisons between our experimental results and those obtained by the traditional phase-shifting algorithm and between our experimental results and those measured by the Fizeau interferometer are made ...
An approach based on two-dimensional iterative nonlinear regression for retrieving phase information from single-frame interferograms was formulated and tested for fluid- and heat-flow measurements. Even though an initial crude phase assignment-i.e., fringe-order numbers at limited data points-is needed, the approach does not require complete phase unwrapping as in conventional techniques. Testing of computer-simulated and real interferometric data shows stable convergence and accurate phase extraction. The method works well under a high noise level, including broken fringes or contaminated regions, with a good noise-cleansing capacity. It provides accuracy at image- or opaque-object boundaries and directly offers spatial-gradient values. A weakness, however, can be intensive computation in the iterative estimation. The method is a good candidate for single-frame interferogram reduction.. © 1998 Optical Society of America. Full Article , PDF Article ...
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Having to reach ever lower limits of detection is a daily challange in modern laboratories. In order to succeed in obtaining sufficiently sensitive analysis methods, sample preparation techniques such as Solid Phase Extraction (SPE) or Liquid-Liquid Extraction are often used as concentration steps. The concentration factor achieved in these cases depends on the amount of solvent used for analyte elution from the SPE cartridge or for liquid extraction. Following the extraction step, further concentration of analytes can be achieved by reducing the amount of solvent left in the extract. This is typically achieved by evaporation. Such a concentration step can contribute significantly to improved limits of detection for the overall analytical method. For the evaporative concentration step, commercially available rotary evaporators as well as custom solutions are widely used. These are mainly stand-alone systems for manual operation. The GERSTEL MultiPosition Evaporation Station (mVAP) in combination ...
TY - CONF. T1 - A simplified SPE procedure combined with HPLC-ESI/MS for phospholipid determination. AU - Fauland, Alexander. AU - Trötzmüller, Martin. AU - Guo, Xinghua. AU - Köfeler, Harald. AU - Lankmayr, Ernst. PY - 2012/3/6. Y1 - 2012/3/6. M3 - (Old data) Lecture or Presentation. ER - ...
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Introduction. Chloropropanol is a kind of toxic carcinogenesis generated in chemical production of soy sauce. It also has inhibitory effect on the production of male hormones which decrease the reproductive ability. It is greatly harmful to human body. Daily common three kinds are as following: 1-chloro-2-propanol (ClCH2CHOHCH3); 3-chloro-1,2-propanediol (3-MCPD) and 1,3- dichloro-2- propanol (1,3-DCP).. Experimental. Sample preparation. Accurately weigh 0.1 g chloropropanols standard substance in to100 mL volumetric flask, constant volume to scale by ethyl acetate and get 1 mg/mL stock solution. Gradually dilute the stock solution with acetone to get 1 μg/mL standard working solution. Weigh 290 g sodium chloride, add water to dissolve and dilute to 1000 mL, and then ultrasonic for 20 min. Weighs 2.5 g soy sauce directly loads to the Cleanert MCPD solid phase extraction column, static balance for 10min, wash the column with 15 mL ethyl acetate and collect the eluent. Nitrogen blow the eluent to ...
Metabolomics studies focus on the analysis of small molecules (MW,2000) in biological matrices from micro-organisms, plants, animals, and of human origin. Relatively large sets of samples are processed to allow differentiation between sample types and it is of course critically important to ensure that the analytical variability is lower than the biological variability. In order to achieve this, automating the sample preparation is a good first step, which can contribute significantly towards improving the repeatability of the total analytical procedure.. A typical metabolomics workflow includes extraction, fractionation or clean-up, derivatization, and a concentration step, followed by GC or LC separation and MS detection. In a series of articles, we describe a number of automated methods that are currently applied in our laboratories. In this first article, we focus on extraction and filtration. In a second article, an automatic fractionation procedure based on solid phase extraction will be ...
TY - JOUR. T1 - Online solid phase extraction with liquid chromatography-tandem mass spectrometry for determination of estrogens and glucocorticoids in water. AU - Goh, Shalene Xue Lin. AU - Duarah, Ankur. AU - Zhang, Lifeng. AU - Snyder, Shane A.. AU - Lee, Hian Kee. N1 - Funding Information: This work was supported by the Singapore National Research Foundation under its Environmental & Water Technologies Strategic Research Programme administered by the Environmental & Water Industry Programme Office (EWI) of the Public Utilities Board, Singapore (Grant no. 706-000-016-490/706-000-016-133 ). S.X.L. Goh is grateful for the award of a graduate research scholarship from the EWI. Publisher Copyright: © 2016 Elsevier B.V.. PY - 2016/9/23. Y1 - 2016/9/23. N2 - The present work describes the development of a novel fully automated online solid phase extraction (SPE) coupled with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) using negative electrospray ionisation (ESI) for ...
Solid phase extraction (SPE) is one of the sample preparation methods most widely used by chromatographers, as demonstrated by the numerous published SPE methods found in the literature. Manual SPE cartridge formats can vary from disks through individual cartridges with a range of different volumes to 96-well plates. However, solid phase extraction methods can be tedious and time consuming when performed manually. There is therefore an increasing need for the automation of solid phase extraction methods. A robotic X-Y-Z coordinate autosampler commonly used for sample introduction in GC or HPLC can be used to perform a wide variety of sample preparation techniques using a single instrument and controlling software. The MAESTRO software allows the user to control the automation of solid phase extraction methods. In addition to ease of use and intuitive windows-based programming, the tools to optimize method parameters ensuring efficient sequence creation and maximum sample throughput. The sampler ...
A novel and highly sensitive method for the determination of some heavy metals in skin whitening cosmetics creams using multiwalled carbon nanotubes MWCNTs as solid phase extraction sorbent for the preconcentration of these heavy metals prior to their determination by inductively coupled plasma atomic emission spectrometry is described. Different practical parameters have been thoroughly investigated and the optimum experimental conditions were employed. The developed method was then applied for the determination of arsenic, bismuth, cadmium, mercury, lead and titanium in samples of skin whitening cosmetics. The detection limits under these conditions for As, Bi, Cd, Pb, Hg and Ti were 2.4, 4.08, 0.3, 2.1, 1.8, and 1.8 ng·mL−1, respectively. The relative standard deviations (RSDs) were found to be less than 2.0%. For validation, a certified reference material of NIST SRM 1570a spinach leaves was analyzed and the determined values were in good agreement with the certified values. The recoveries for
|p|This study is focused on the determination of some important antibiotics from different classes in waste water samples using solid phase extraction followed by high performance liquid chromatography with two detectors, diode array and mass spectrometer in positive ionisation mode. The investigated antibiotics include three penicillins (amoxicillin, ampicillin, penicillin G), two cephalosporins (ceftazidime, ceftriaxone), and two tetracyclines (tetracycline, doxycycline). The studied antibiotics were extracted from waste water samples using hydrophilic-lipophilic balanced cartridges. The extraction of antibiotics from water matrices was tested at several pH values. The best recoveries were obtained at pH 3 and 7 respectively. Depending on the nature of antibiotic, the limits of detection and quantification were obtained in the range of 0.07-0.92 µg mL−1 and 0.21-2.77 µg mL−1 respectively. Influent and effluent waste water samples were collected from a Waste Water
China Solid Phase Extraction (SPE) Consumables Market Research Report 2017 is a market research report available at US $3400 for a Single User PDF License from RnR Market Research Reports Library.
TY - JOUR. T1 - A rapid method for extraction of total lipids from whey protein concentrates and separation of lipid classes with solid phase extraction. AU - Vaghela, M. N.. AU - Kilara, A.. PY - 1995/10/1. Y1 - 1995/10/1. N2 - A modified procedure for extraction of total lipids from whey protein concentrates was developed such that stable emulsion with extracting solvents was avoided and the solvent system remained monophasic. Nonlipid contaminants from the extract were removed using gel filtration instead of traditional aqueous washing to prevent any loss of polar lipids. The extraction of total lipids by the modified procedure was complete and comparable with a reference procedure. Traditional thin-layer chromatography is tedious and more qualitative than quantitative for lipid class separation. Total lipids were further separated into free fatty acids, phospholipids, cholesterol ester, triacylglycerol, cholesterol, diacylglycerol, and monoacylglycerol, using modified solid phase extraction ...
Boom method (Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid from a biological sample. This method is characterized by absorbing the nucleic acids (NA) to the silica beads. Boom method (Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid (NA) from biological samples. Essential of this method is the use of silica beads, capable of binding the NA in the presence of a chaotropic substance according to the effect. This method is one of the most widespread methods for isolating nucleic acids from biological samples and is known as a simple, rapid, and reliable method for the small-scale purification of NA from biological sample. This method is said to have been developed and invented by Willem R. Boom et al. around 1990. However, the aforementioned chaotropic effect itself was already known and already reported by Vogelstein and Gillespie before the development of the BOOM method. So the contribution ...
從台灣市場購得舒黨Su-Dangshen (Codonopsis tubulosa)和紋黨Wun-Dang.(C. pilosula),分別用水萃取後,再經solid phase extraction (SPE) 濃縮乾燥,利用核磁共振光譜儀(1H-NMR)分析時,從舒黨發現兩個新四級銨鹽codotubulosine A和codotubulosine B 生物鹼。本篇論文將台灣及中國大陸市場收集各類黨參樣品共42個,利用1H-NMR分析定量各樣品中codotubulosine A、codotubulosine B 生物鹼及adenosine 和5-(hydroxymethyl)furfural之含量。分析結果在黨參、素花黨參、川黨參中以codotubulosine A 為主要成分,在管花黨參中以codotubulosine A 和B 為主要成分,新疆黨參、羊乳、金錢豹和球花黨參均未發現 codotubulosine A 和 B;目前台灣及中國大陸市場常用藥材,將黨參、舒黨、素花黨參和川黨參視為主要商品。因此本論文將codotubulosine A 和 B生物鹼,建議作為黨參之指標成分。利用SPE結合1H-NMR可快速鑑定市售黨參藥材的品種
A fully automated and validated 2D online solid-phase extraction (SPE) high-performance liquid chromatographic method was described for the direct simultaneous determination of clenbuterol (CLEN) and doxazosin (DOX) in spiked human plasma samples. Chromatographic separation of the analytes was achieved by column-switching. SPE column packed with restricted access material for depleting matrix and a reversed phase HPLC column for eluting analytes were used with UV detection on 250 nm. A 10 mM acetate buffer (pH: 4) and acetonitrile mixture was used in gradient elution with 1 mL/min flow rate, as mass spectrometry friendly mobile phase. Calibration graphs were prepared for spiked plasma, and good linearity was achieved over a dynamic range of 75.0 ng/mL-200.0 mu g/mL for CLEN and 37.5 ng/mL-100.0 mu g/mL for DOX. Detection and quantification limits were 8.47 and 11.94 ng/mL, 25.66 and 36.18 ng/mL for CLEN and DOX, respectively. Online column-switching liquid chromatography technique combines the ...
An extraction cartridge, for extracting a component from a composition, contains a packing composition including a buffer composition, diatomaceous earth and absorbent. The cartridge is made by heating each component to assure it is free from contaminants. The diatomaceous earth is sifted to obtain a particular particle size. The cartridge also includes frits and filters for holding the packing composition within the cartridge. All of the components of the cartridge are selected or processed to assure that no contaminants are leached into the sample being extracted. A processing system automatically processes a plurality of cartridges. The processing system, which is capable of continuous operation, includes a platform, a rack for holding the cartridges, sample vials and extraction tubes. A walking beam conveyor system moves the rack along the platform and a system of robotic arms automatically transfers the sample to be extracted from the sample vial to the extraction cartridge and adds a predetermined
ISOLUTE® NH2 solid phase extraction cartridges work well for detecting alcohol biomarkers in human urine, and a recent study reported a successful throughput of 6,000 samples per year. The key advantage to this method is the elimination of the evaporation step prior to analysis by LC-MS/MS.. A new application note from Biotage (AN818.v1) offers some suggestions to optimize this method further, and also verifies the method on patient samples. The ISOLUTE NH2 cartridge format demonstrated as a viable option for urine measurements over a relevant concentration range in clinical reference laboratories.. ...
TY - JOUR. T1 - A case of death after ingestion of an agrochemical spreading agent. AU - Usui, Kiyotaka. AU - Hayashizaki, Yoshie. AU - Okubo, Yoshikazu. AU - Hashiyada, Masaki. AU - Funayama, Masato. PY - 2011/7/15. Y1 - 2011/7/15. N2 - An agrochemical spreading agent was found near the slightly decomposed corpse of a deceased female. The appearance of the stomach contents suggested that ingestion of a surfactant had occurred before death. The spreading agent was found to contain nonionic nonylphenol ethoxylates (NPEOn) and anionic sulfonated naphthalene-formaldehyde condensates (SNFCn). A solid phase extraction cartridge containing a mixed reversed phase-weak anion exchange sorbent (Oasis WAX, Waters) was used to successfully extract both NPEOn and SNFCn from the blood. The cartridge was preconditioned with methanol and acetic acid (AcOH). After the dilute blood sample was applied to the cartridge, it was washed with AcOH, and then NPEOn and SNFCn were eluted with methanol/dichloromethane ...
Eligible patients acquired their individualized dose of MTX on day 1 and blood samples had been collected for 48 h, until day 3, for your evaluation of MTX. Sufferers obtained thirty mg CP 690,550 every single twelve h from day 3 till day 6. On day 6, serial blood samples have been taken for examination STAT inhibitors of CP 690,550. On day 7, patients received their weekly MTX dose mixed with a 30 mg dose of CP 690,550, blood samples had been collected for your following 48 h for evaluation of CP 690,550 and MTX. Blood samples for PK examination of CP 690,550 were collected on day 1 at 0 h, days 6 and 7 at 0, 0. 25, 8 and twelve h, and also at 24 and 48 h publish day 7 dosing. Blood samples for PK examination of MTX have been collected on days 1?3 and days 7?9 at 0, 24 and 48 h.. Samples had been analysed for CP 690,550 concentrations making use of validated solid phase extraction followed by liquid chromatography/tandem mass spectrometry methodology. Samples had been analysed for MTX ...
Charlène Pouech, Florent Lafay, Laure Wiest, Robert Baudot, Didier Léonard, et al.. Monitoring the extraction of additives and additive degradation products from polymer packaging into solutions by multi-residue method including solid phase extraction and ultra-high performance liquid chromatography-tandem mass spectrometry analysis. Analytical and Bioanalytical Chemistry, Springer Verlag, 2014, 406 (5), pp.1493-1507. 〈10.1007/s00216-013-7551-4〉. 〈hal-00924245〉 ...
Abstract. INTRODUCTION: C-peptide is a widely used marker of endogenous insulin secretion. It is used for assessing residual beta cell function and in the diagnostic workup of hypoglycemia. C-peptide is routinely measured in the clinical laboratory by immunoassays, which are sensitive but prone to limitations such as cross-reactivity, between-lot variability, and a lack of concordance across different platforms. LC-MS/MS methods are more specific and can be multiplexed. Previous LC-MS/MS methods developed for serum c-peptide measurement detected intact peptide. Unfortunately, intact peptide is poorly ionized, requiring immunoaffinity extraction or solid phase extraction with two-dimensional chromatography. OBJECTIVE: The objective of this study was to develop and validate a novel enzyme (Glu-C) digestion-based LC-MS/MS assay for the quantification of serum c-peptide. METHODS: The internal standard was isotopically labeled at two leucine residues: EAED*LQVGQVELGGGPGAGSLQP*LALEGSLQ. Serum proteins ...
A sensitive method has been developed for the trace analysis of PAHs and their oxidation products (i.e., nitro-, oxy-, and hydroxy-PAHs) in air particulate matter (PM). Following PM extraction, PAHs, nitro-, oxy-, and hydroxy-PAHs were fractionated using solid phase extraction (SPE) based on their polarities. Gas chromatography-mass spectrometry (GC-MS) conditions were optimized, addressing inject ...
HPLC: high performance liquid chromatography; UV: ultraviolet detection; CE: capillary electrophoresis; FLD: fluorimetric detection; MS: mass spectrometry; MS/MS: tandem mass spectrometry; SPE: solid phase extraction; PLE: pressure liquid extraction ...
A simple solventless injection method was introduced based on the using of a solid-phase microextraction (SPME) fiber for injection of large amounts of the analytes extracted by the magnetic solid phase extraction (MSPE) procedure. The resulted extract from MSPE procedure was loaded on a G-coated SPME fiber, and then the fiber was injected into the gas chromatography (GC) injection port. This method combines the advantages of exhaustive extraction property of MSPE and the solvent-less injection of SPME to improve the sensitivity of the analysis. In addition, the analytes were re-concentrated prior to inject into the gas chromatography (GC) inlet because of the organic solvent removing from the remaining extract of MSPE technique. Injection of the large amounts of analytes was made possible by using the introduced procedure. Fourteen polycyclic aromatic hydrocarbons (PAHs) with different volatility were used as model compounds to investigate the method performance for volatile and semi-volatile ...
A fast and simple modified QuEChERS extraction method was developed for determination of Benzo[a]pyrene (BaP) in 137 traditional (Sangak), semi-industrial (Sangak) and industrial bread samples using spiked calibration curves by GC/MS. Sample preparation includes extraction of BaP into acetone followed by cleanup with dispersive solid phase extraction. The limit of detection and limit of quantification were 0.3 ng/g and 0.5 ng/g, respectively. The values for recoveries and RSD were calculated as 110.5-119.85% and ,11.6% respectively. Average concentration of BaP in Sangak bread samples of Shiraz and Tehran was 0.59 and 0.60 ng/g, respectively. 35.5% of samples of breads collected in Tehran were contaminated with BaP at the amount higher than maximum levels regulated in processed cereal-based foods and baby foods by European Commission (1 ng/g). Seventeen percent of samples of breads collected in Shiraz were contaminated with BaP which 13 % of total samples were ,1 ng/g. BaP content in all ...
Quantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane-ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false ...
Researchers at Griffith University in Australia have developed a new diffusive gradients in thin films (DGT) method to determine the concentration of antimony in polluted waters. This new technique also allows them to measure in situ the speciation of this metalloid in its different oxidation states. Speciation could be carried out before using solid phase extraction (SPE) cartridges, but these present some limitations when it comes to analysing some complex mixtures like seawater.. On the other hand, DGT is a sampling technique that can be used to measure a myriad of analytes in different mixtures. DGT uses a combination of hydrogel-based layers to extract and retain the analyte, that can be eluted and determined later on. This technique has been used to measure trace metals, metalloids, sulphides, phosphates and ammonium. In this case, to enhance the affinity of DGT membranes to antimony, researchers have used thiol-based silica gel layers.. In this paper, the authors present a combination of ...
Biotage QuEChERS products include extraction tubes for the initial extraction and partitioning of analytes from homogenized food samples, and clean up tubes for the dispersive solid phase extraction. All Biotage QuEChERS products come packed in a rack for ease of storage and use on the bench. Additionally, solvent resistant paper labels aid hassle free sample indentification. Biotage supplies pre-weighed extraction and clean up tubes that conform with AOAC1,2 and the EU3 published methods, including those for waxed and highly pigmented fruit and vegetable samples. ...
Between classes, Westerman would dash off to the lab in Thompson Hall to thaw waste-water samples in a water bath. In the afternoons, she prepared the samples in the trace analysis lab, where light streamed in from windows overlooking the glass-walled Oppenheimer Café and the Brown Family Courtyard. Decked out in lab coat, gloves, and safety glasses, and with Johnny Cash belting out mournfully through a speaker, Westerman would begin by putting a small volume of thawed wastewater samples through a filter to clean out the solids. It definitely didnt smell great, Westerman remembers. But it was a lot more dilute than the contents of toilet flush, she adds, and youd be surprised how quickly you get used to it. For the focused work of solid phase extraction, the next step of sample preparation, Westerman would fold her 6-foot-1-inch frame onto a lab stool in front of the fume hood. There, she put small amounts of the cloudy liquid through an array of tubes that looked like upside-down ...
This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using ISOLUTE® SAX strong anion exchange solid phase extraction plates, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. Very clean extracts are possible due to the purely anion exchange retention mechanism available using this silica based sorbent. This minimizes the co-extraction of endogenous interferences through hydrophobic (or non-polar) retention. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included ...
Problem] To provide a polymer which enables the solid phase extraction of a metal ion, particularly a rare earth element, and from which a metal of interest can be collected by burning the polymer after the adsorption of the metal of in ...
A broad range of organic compounds is recognized as environmentally relevant for their potential adverse effects on human and ecosystem health. This method was developed to better determine the distribution of 61 compounds that are typically associated with industrial and household waste as well as some that are toxic and known (or suspected) for endocrine-disrupting potential extracted from environmental sediment samples. Pressurized liquid extraction (PLE) coupled with solid-phase extraction (SPE) was used to reduce sample preparation time, reduce solvent consumption to one-fifth of that required using dichloromethane-based Soxhlet extraction, and to minimize background interferences for full scan GC/MS analysis. Recoveries from spiked Ottawa sand, commercially available topsoil, and environmental stream sediment, fortified at 4-720 ??g per compound, averaged 76 ?? 13%. Initial method detection limits for single-component compounds ranged from 12.5 to 520 ??g/kg, based on 25 g samples. Results from
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Polar Strong Anion (PSA) Exchange - AccuBONDII Sorbents for SPE Chromatography, Agilent Technologies : Bulk sorbents for packing SPE cartridges or to
α-Cobratoxin (CTX) is a large peptide (71 amino acids) with strong analgesic effect and may be misused in sports such as horse racing. To prevent such misuse, a sensitive method is required for detection and confirmation of the toxin in equine samples. CTX was extracted from equine plasma using an optimized mixed-mode solid-phase extraction (SPE) procedure. Extracted CTX was reduced with dithiothreitol and alkylated with iodoacetamide, and then was digested by trypsin at 56 C for 30min. The digest was analysed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), and tryptic peptides T2 ( CFITPDITSK ) and T4 ( TWCDAFCSIR ) were monitored for detection and confirmation of CTX. The limit of detection (LOD) was 0.05ng/mL for CTX in plasma, and the limit of confirmation (LOC) 0.2ng/mL. Unlike small peptides consisting of the 20 canonical amino acids, CTX was stable in equine plasma at ambient temperature for at least 24h. The developed analytical method was successfully applied ...
Thermo Scientific™ HyperSep™ C18 Cartridges 5g Bed Weight; 25mL Column Volume Thermo Scientific™ HyperSep™ C18 Cartridges SPE Columns
Shop a large selection of SPE Columns products and learn more about Thermo Scientific HyperSep Cyano Cartridges 100mg Bed Weight; 1mL Column 100mg Bed Weight; 1mL Column Volume.
The skin sensitisation property of the registration substance, sodium N-lauroyl glutamate, is derived based on the read-across to sodium N-cocoyl glutamate and sodium N-cocoyl glycinate. The registration substance and the read-across sources are amides of fatty acids and amino acids and can be characterized as N-fatty acyl amino acids, of which endogeous occurence and metabolism are known. Based on the comparable chemical structure, comparable phys-chem data and expected comparable metabolism, these compound are likely exhibit comparable toxicity profiles. Sodium N-cocoyl glutamate was investigated for its skin sensitization property according to the OECD 406 (Buehler method). 0.5 ml of the test material wasoccusively applied to the clipped skin of guinea pigs for 6 hours as induction and challenge treatment. None of the twenty treated animals responded. Sodium N-cocoyl glutamate is not skin sensitizing. Sodium N-cocoyl glycinate was investigated for its skin sensitization property according ...
This thesis focuses on the use of molecularly imprinted polymers as selective sorbents for solid-phase extraction (MISPE). The MISPE methods developed were mainly intended for use with biological samples, such as human urine and blood plasma. These body fluids are complex samples, which often need an effective clean-up step before analysis to reduce the levels of possible interfering substances from the matrix, especially if the analytes are present in trace amounts. Solid-phase extraction (SPE) is a well-established and routinely used method for clean-up and preconcentration of samples from diverse matrices. However, conventional SPE sorbents often lack selectivity, leading to co-extraction of interferences, which negatively affects the following detection method. One of the advantages of MISPE is the built-in selectivity for a target analyte, or class of structurally related analytes, enabling the efficient clean-up that is often required for biological samples. The built-in selectivity of ...
CHROMFILTER is a Hongkong company composed by a well-trained working team having experiences in this profession for more than 20 years. As an excellent HPLC consumable products manufacturer, supplier and exporter, we have a factory in Songjiang District, Shanghai City. Our main products include all kinds of chromatography (GC/HPLC), laboratory filtration and laboratory accessories.CHROMFILTER designs, manufactures, distributes and sells a variety of laboratory products including syringe filters and membranes; sample vials and caps; SPE column; Disposable Vacuum Filtration system; sealing matts and other GC/HPLC high quality glass and plastic supplies.
CHROMFILTER is a Hongkong company composed by a well-trained working team having experiences in this profession for more than 20 years. As an excellent HPLC consumable products manufacturer, supplier and exporter, we have a factory in Songjiang District, Shanghai City. Our main products include all kinds of chromatography (GC/HPLC), laboratory filtration and laboratory accessories.CHROMFILTER designs, manufactures, distributes and sells a variety of laboratory products including syringe filters and membranes; sample vials and caps; SPE column; Disposable Vacuum Filtration system; sealing matts and other GC/HPLC high quality glass and plastic supplies.
This page lists B&O compatible phono cartridges, for other turntables we have a wide range of solutions see drop-down menus on page top for all our cartridges, audio products and services.. You have come to the best place for a solution. We are the only manufacturer of these cartridges, and we are authorized to do so under license by Bang and Olufsen. They are fully warranteed against manufacturing defect for one full year. Want to upgrade your cartridge and performance? Easily done. The B&O plug-in replacement cartridge design allows use of an interchangeable and wide range of cartridge models and performance for each turntable model - within each of the two groups below. (Group 1 = SMMC1, SMMC2, SMMC3, SMMC4 and The Voice; Group 2 = SMMC20E, SMMC20EN, SMMC20CL and SMMC20CL+) PLEASE NOTE; Use our model lookup tool to see which group your turntable needs. Cartridges purchased and opened (broken seal) cannot be returned for refund.. Do you want to use a B&O cartridge (Group 1) on a NON-B&O ...
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This article discusses the benef ts of using automated dispersive solid-phase extraction (dSPE) rather than standard SPE in urine analysis. ...
Solid-phase extraction for simultaneous separation and preconcentration of Fe(III) and Zn(II) traces using three chelatants and Ramelak bark-derived activated carbon as a new bio- ...
A fluid pumping assembly for particular use in delivery of sterile fluid used in surgical procedures, includes a pump control structure to which a replaceable fluid pumping cartridge is connected and disconnected to provide convenient replacement and disposability of the cartridge. The pump cartridge is self-contained in that it includes a fluid pump which is adapted for automatic connection to a pump drive structure when the cartridge is inserted into the assembly, so that the sterile fluid is pumped only through the pumping cartridge and fluid flow is entirely isolated from the pump control structure to ensure that the fluid is maintained sterile during pumping. The cartridge can include a selectively activated pulsation control to enable discharging the fluid from the cartridge in either a continuous flow or pulsating flow of the fluid. The cartridge can also include fluid flow dampening and safety vent features. The pump control structure can provide a pneumatic drive system which cycles pressurized