Mung bean nuclease is a nuclease derived from sprouts of the mung bean Vigna radiata that removes nucleotides in a step-wise manner from single-stranded DNA molecules (ssDNA) and is used in biotechnological applications to remove such ssDNA from a mixture also containing double-stranded DNA (dsDNA). This enzyme is useful for transcript mapping, removal of single-stranded regions in DNA hybrids or single-stranded overhangs produced by restriction enzymes, etc. The enzyme degrades single-stranded DNA or RNA to nucleoside 5-monophosphates, but does not digest double-stranded DNA, double-stranded RNA, or DNA / RNA hybrids. Mung Bean Nuclease catalyzes the specific degradation of single-stranded DNA or RNA, and produces mono and oligonucleotides carrying a 5′-P terminus. Mung bean nuclease has a theoretical molecular weight of 39 kDa. Mung bean nuclease has a stringent single-stranded specificity for DNA or RNA and produces 5-phosphoryl oligo- and mononucleotides. Mung bean nuclease requires ...
In my hands, Mung Bean S1 Nuclease is easier to use than Nuclease S1. Mung bean is a single-strand-specific nuclease purified from germinated Mung bean sprouts. Degrades DNA and RNA to 5-phosphoryl mononucleotides. (S. C. Sung and M. Laskowski Sr. 1962 J. Biol. Chem. 237: 506.) Single polypeptide chain MW 39,000. Mung bean nuclease is the enzyme of choice over S1 Nuclease for applications where its low activity on dsDNA is desirable. Ratio of activity on ssDNA versus dsDNA is greater than 1500:1 (measured by hyperchromicity by USB ...
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PNA and single-strand-specific nuclease were used here for SNPs detection. After treatment by single-strand-specific nuclease such as S1 and mung bean, the DNA strand in PNA/DNA duplex, which involves a one-base mismatch, was completely digested. However,
We report, for the first time, the N-terminal amino acid sequences of both intact and cleaved forms (fragments A and B) of Mung bean nuclease, purified from sprouts of Vigna radiata or purchased from Amersham Biosciences ...
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Investigating steric protection of DNA in the presence of nucleases. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
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Mung beans have been a staple of the cuisines of India, China, Korea and Southeast Asia for thousands of years. And with good reason. They are a delicious and healthy source of protein and fiber.In th...
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I dont mind the flashbacks. Its that old acid mung that gets me down - Меня не так беспокоят глюки после кайфа от ЛСД, а вот это липкое состояние
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Know nutrition and calorie facts in 100gms of Mung beans, mature seeds, cooked, boiled, with salt. Includes - total fat, carbohydrates, proteins, vitamins and minerals.
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Strains: Strains of S. cerevisiae used in this study are listed in Table 1. To generate strain MF21 the plasmid pPM19 was used. pPM19 was constructed as follows. First, pYSL1 was digested with XhoI and ClaI, and the 5.3-kb fragment that contains the EXO1 gene was ligated into the XhoI-ClaI site of pGEM7 (Promega, Madison, WI). The resulting plasmid, pPM18, was digested with SalI and EcoRI, which removes the internal 1.2-kb fragment of EXO1, and the protruding DNA termini was converted to blunt ends by treatment with mung bean nuclease. Subsequently, plasmid pFL34 was digested with BglII, and the resulting 3′ recessed ends of the 1.1-kb fragment containing URA3 were filled with the Klenow fragment of DNA polymerase I. The fragment was used to replace the deleted fragment of EXO1 of the plasmid pPM18, generating plasmid pPM19. Subsequently, pPM19 was digested with XhoI and ClaI, and the resulting 4.9-kb fragment containing the exo1::URA3 disruption was transformed into SSL204. The correct ...
Mung Bean Planting and Harvesting. Mung beans, or Vigna radiata, a warm-season legume native to India, are grown today in Asia, Africa, South America and the United States. You might best recognize mung beans as the bean sprouts served in salad bars and used in Asian cooking. Mung beans are grown as a commercial field ...
The anti-inflammatory effect of mung bean ethanol extract on lipopolysaccharide (LPS) stimulated macrophages (J774) was evaluated. The mung bean extract was separated into 5 fractions by normal phase
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5202 Pancreatic cancer represents a leading cause of cancer-related mortality, with only limited (,5 years) survival rate. Cigarette smoking has been implicated in the development of pancreatic cancer. Because of the high mortality rate, it is necessary that early biomarkers be developed before the onset of pathological changes. In this study, we analyzed highly lipophilic adducts in the pancreas DNA of non-smokers (n=7.) and smokers (n=8). Tissues were obtained from the U.S. Cooperative Tissue Network, and the isolated DNAs were analyzed by nuclease P1-mediated 32P-postlabeling/TLC. When chromatographed in standard high-salt, high-urea solvents, adducts migrated in the form of diagonal radioactive zones in both non-smokers and smokers; however, the adduct load was significantly (p=0.031) higher in smokers (231±38 adducts/109 nucleotides, mean±SE) compared with non-smokers (94±42 adducts/109 nucleotides). To determine the chemical nature of the adducts, selected smoker DNA samples were ...
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Mung beans, mature seeds, sprouted, cooked, stir-fried - nutrtion information: calories, carbohydrates, protein, fat and other nutritional food data
DA wrote: , What are the general opinions of the group regarding , digesting tissue culture cell preparations with , nucleases during sample prep for SDS-PAGE. If one , doesnt add nucleases, are there physical or chemical , strategies to make samples less viscous? , Thanks for any thoughts. , David You could sonicate, or run the lysate through a small bore needle (e.g., 25g -ish) and syringe a few times. Nick -- Nick Theodorakis nick_theodorakis at hotmail.com contact form: http://theodorakis.net/contact.html ...
This article looks into some of the features of the free SABR model, in particular in the context of the negative-rate environment.
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Place all but seasoning in 5 quart pan, add water almost to top, simmer until mung beans are done, adding water as needed to keep pot full. Add Greek seasoning mixture only when you are certain the beans are done to your liking.. ...