Simian virus (SV40) large T antigen, molecular model. This antigen is from the simian vacuolating virus 40 (SV40). Large T antigens play a role in regulating the viral life cycle of the polyomaviridae viruses, such as SV40. SV40 is found in monkeys such as Rhesus monkeys and macaques. Potentially tumour-causing in primates and humans, it is used in laboratory research and in vaccines. - Stock Image C025/1808

This topic contains 11 study abstracts on Simian virus 40 (SV40) indicating it may contribute to Simian virus 40 (SV40), Mesothelioma, and Cancer Metastasis

Simian virus 40 (SV40)-mediated transformation of human diploid fibroblasts has provided an effective experimental system for studies of both senescence in cell culture and carcinogenesis. Previous interpretations may have been complicated, however, by the semipermissive virus-cell interaction. In earlier studies, we previously demonstrated that the human diploid fibroblast line HS74 can be efficiently transformed by DNA from replication-defective mutants of SV40 containing a deletion in the viral origin for DNA synthesis (SVori-). In the current study, we found that such SVori- transformants show a significantly increased life span in culture, as compared with either HS74 or an independent transformant containing an intact viral genome, but they nonetheless undergo senescence. We have clonally isolated six immortalized derivatives of one such transformant (SV/HF-5). Growth studies indicate that the immortalized cell lines do not invariably grow better than SV/HF-5 or HS74. Genetic studies ...

In this report we present evidence that simian virus 40 T antigen encodes a biological activity that is functionally equivalent to the transforming activity lost by deletion of the E1A p300-binding region. T-antigen constructs from which the pRb-binding region has been deleted are virtually unable to induce foci of transformed cells in a ras cooperation assay in primary baby rat kidney cells. Nevertheless, such a construct can cooperate with an E1A N-terminal deletion mutant, itself devoid of transforming activity, to induce foci in this assay. The heterologous trans-cooperating activity observed between E1A and T-antigen deletion products is as efficient as trans cooperation between mutants expressing individual E1A domains. The cooperating function can be impaired by a deletion near the N terminus of T antigen. Such a deletion impairs neither the p53-binding function nor the activity of the pRb-binding region.. ...

The oligomers formed by a mutant nonkaryophilic large T antigen of simian virus 40, which lacks residues 110 through 152 of normal large T antigen and transforms only established cells (L. Fischer-Fantuzzi and C. Vesco, Proc. Natl. Acad. Sci. USA 82:1891-1895, 1985), were found to consist predominantly of dimers. Anti-p53 antibodies precipitated 14 to 16S complexes containing the mutant nonkaryophilic large T antigen and p53 from extracts of transformed cells, and anti-p53 indirect immunofluorescence stained these cells in the cytoplasm. ...

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The role of simian virus 40 (SV40) large tumor antigen (T antigen) as a DNA helicase at the replication fork was studied. We found that a T-antigen hexamer complex acts during the unidirectional unwinding of appropriate DNA substrates and is localized directly in the center of the fork, contacting the adjacent double strand as well as the emerging single strands. When bidirectional DNA unwinding, initiated at the viral origin of DNA replication, was analyzed, a larger T-antigen complex that is simultaneously active at both branch points of an unwinding bubble was observed. The size and shape of this helicase complex imply that the T-antigen dodecamer complex, assembled at the origin and active in the localized melting of duplex DNA, is subsequently also used to continue DNA unwinding bidirectionally. Then, however, the dodecamer complex does not split into two hexamer subunits that track along the DNA; rather, the DNA is threaded through the intact complex, with the concomitant extrusion of ...

You searched for: Creator Nathans, Daniel, 1928-1999 Remove constraint Creator: Nathans, Daniel, 1928-1999 Creator Lee, Theresa N. H. (Theresa N. H. Lee) Remove constraint Creator: Lee, Theresa N. H. (Theresa N. H. Lee) Subject DNA Cleavage Remove constraint Subject: DNA Cleavage Subject Simian virus 40 Remove constraint Subject: Simian virus 40 ...

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Deppert, W and Walter, G, Domains of simian virus 40 large t-antigen exposed on the cell surface. (1982). Subject Strain Bibliography 1982. 3601 ...

We previously analyzed human embryonic kidney (HEK) cell lines for the effects that simian virus 40 (SV40) small tumor antigen (ST) has on gene expression using Affymetrix U133 GeneChips. To cross-validate and extend our initial findings, we sought to compare the expression profiles of these cell lines using an alternative microarray platform. METHODS: We have analyzed matched cell lines with and without expression of SV40 ST using an Applied Biosystems (AB) microarray platform that uses single 60-mer oligonucleotides and single-color quantitative chemiluminescence for detection. RESULTS: While we were able to previously identify only 456 genes affected by ST with the Affymetrix platform, we identified 1927 individual genes with the AB platform. Additional technical replicates increased the number of identified genes to 3478 genes and confirmed the changes in 278 (61%) of our original set of 456 genes. Among the 3200 genes newly identified as affected by SV40 ST, we confirmed 20 by QRTPCR including

The genome of Simian Virus 40 (SV40) is a covalently closed circular DNA duplex with about 5200 nucleotide pairs. Based on physiological studies and mapping of temperature-sensitive and deletion...

We have analysed nucleotide sequences at the junction between simian virus 40 (SV40) and cellular DNA in the Fisher rat transformed line tsA30-N2. This line

simian virus 40, polio vaccines, and cancer I take no pleasure in these essays that expose the poor judgment and even recklessness of the US medical establishment. But, facts are facts, albeit unpleasant in many instances. (Update: Summer, 2014. We have recently heard that the introduction of anti-biotics in the early 1940s also seems to…

This entry was posted on June 2, 2011, 3:42 am and is filed under Fugitive Document. You can follow any responses to this entry through RSS 2.0. You can leave a response, or trackback from your own site. ...

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in Research in Veterinary Science (2009), 87(1), 123-32. In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be ... [more ▼]. In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be distinguished from simultaneously isolated colonocytes, as the jejunocytes specifically exhibited the small intestinal peptidase gene transcript, as well as an active alkaline phosphatase. The transformation of both types of cell suspension was performed by retroviral infection, using reproduction-defective viruses bearing the gene coding for the large T antigen of the leukaemia simian virus (SV40). The success of the transfection was demonstrated by (1) a significant increase in cell passage numbers (52-53 vs. 7 passages ...

4FGN: Analysis of the Costructure of the Simian Virus 40 T-Antigen Origin Binding Domain with Site I Reveals a Correlation between GAGGC Spacing and Spiral Assembly.

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TY - JOUR. T1 - Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription. AU - Nakagoshi, H.. AU - Nagase, T.. AU - Kanei-Ishii, C.. AU - Ueno, Y.. AU - Ishii, S.. PY - 1990/3/23. Y1 - 1990/3/23. N2 - The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The c-myb protein purified from the human T-cell line, Molt4, was also shown to recognize the same sequene. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of a c-myb-binding sequence were shown to function as a c-myb-dependent enhancer. These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription.. AB - The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The ...

TY - JOUR. T1 - Transformation of DNA repair-deficient human diploid fibroblasts with a simian virus 40 plasmid. AU - Wood, C. M.. AU - Timme, T. L.. AU - Hurt, M. M.. AU - Brinkley, B. R.. AU - Ledbetter, D. H.. AU - Moses, R. E.. PY - 1987/4. Y1 - 1987/4. N2 - Fibroblasts from patients with xeroderma pigmentosum (XP) complementation groups A, C, D, E, and G, as well as Bloom syndrome (BS) and Fanconi anemia (FA) have been transfected with a plasmid, pSV7, containing the early region of Simian virus 40 (SV40). All of the cultures exhibited cytologic changes characteristic of transformed cells and expressed T-antigen. They also contained integrated copies of DNA derived from the vector, and in several cases, extrachromosomally replicated DNA. Not all of the transfected cultures became immortalized. The transformed xeroderma pigmentosum (XP) cultures retained their UV-sensitive phenotype in all but one case. The BS and FA cell lines retained their characteristic phenotype. All of the cultures, ...

DELETION OF THE CARBOXY TERMINUS OT SIMIAN VIRUS 40 LARGE T ANTIGEN AFFECTS VIRAL LATE GENE EXPRESSION A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy Terryl Stacy DARTMOUTH COLLEGE Hanover, New Hampshire March 8,1990 ...

Lambda-Tris(4,7-diphenyl-1,10-phenanthroline)cobalt(III), a photoactivated DNA-cleaving agent, is a small molecular probe of DNA structure. Because of its chirality, the complex cannot bind to regular right-handed B-form DNA but exhibits site-specific cleavage along the polymer strand at conformationally distinct sites such as those in a left-handed conformation. Both coarse and higher resolution mapping experiments using the chiral cobalt complex indicate intriguing conformational variations along the simian virus 40 genome. Highly specific cleavage is evident in the enhancer and promoter blocks and in the region downstream of 3 termini. A specific cleavage pattern borders an alternating purine/pyrimidine stretch within the enhancer, which was found earlier to bind anti-Z-DNA antibodies. Throughout the simian virus 40 genome, variations in structure delineated with the cobalt complex appear to correlate with regions important for control of gene expression. ...

Li J.J., Kelly T.J.. We recently described a soluble cell-free system derived from monkey cells that is capable of replicating exogenous plasmid DNA molecules containing the simian virus 40 (SV40) origin of replication (J.J. Li, and T.J. Kelly, Proc. Natl. Acad. Sci. U.S.A. 81:6973-6977, 1984). Replication in the system is completely dependent upon the addition of the SV40 large T antigen. In this report we describe additional properties of the in vitro replication reaction. Extracts prepared from cells of several nonsimian species were tested for the ability to support origin-dependent replication in the presence of T antigen. The activities of extracts derived from human cell lines HeLa and 293 were approximately the same as those of monkey cell extracts. Chinese hamster ovary cell extracts also supported SV40 DNA replication in vitro, but the extent of replication was approximately 1% of that observed with human or monkey cell extracts. No replication activity was detectable in extracts ...

Summary Simian virus 40 (SV40)-transformed cells express the SV40-specific tumour transplantation antigen (TSTA) on the cell surface and the SV40-coded tumour antigen in their nuclei. TSTA is defined by SV40-specific transplantation immunity, whereas T-antigen (T-Ag) can be detected serologically by indirect immunofluorescence. Both antigens, however, are derived from the A gene of SV40. We therefore analysed SV40-transformed cells for the presence of serologically detectable T-Ag-related molecules. Such antigens could not be detected on the surface of living SV40-transformed cells in monolayers. However, after a short formaldehyde fixation it was possible to stain the cell surfaces of SV40-transformed cells with sera from rabbits immunized with purified SDS-denatured T-Ag, but not with sera from hamsters bearing SV40-induced tumours. T-Ag-related antigens could be detected with both types of antisera by applying a more sensitive 125I-protein A assay. The T-Ag specificity of the binding of hamster SV40

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The complete nucleotide sequence of the P gene of simian virus 41 (SV41) was determined. The gene was found to be 1406 nucleotides long and to contain a relatively small open reading frame encoding a cysteine-rich V protein with a calculated M r of 24076. We have demonstrated that RNA-editing events occur in SV41 P gene transcripts and that the ratio of edited mRNAs to faithfully copied mRNA (P-mRNA: V-mRNA) is about 1:5 at either 24 or 40 h post-infection. The mRNA with two G insertions was capable of encoding a P protein of 395 amino acids with a predicted M r of 41992. A kinetic study of P and V proteins by Western blot analysis showed that in virus-infected cells the amounts of both proteins were almost equal although the V-mRNA was considerably more abundant than the P-mRNA. Alignment of the SV41 P and V proteins with those of nine other paramyxoviruses demonstrated that irregular gaps were present around the RNA-editing sites.

By Catherine Frompovich After half a century, the 108th U.S. Congress House of Representatives Subcommittee on Human Rights and Wellness finally held a hearing September 10, 2003, on the simian virus (SV40) that was included in the original polio vaccine produced and administered to children in the 1950s and 1960s. Candidly, the first page of ...

SV40 and/or DNA sequences indistinguishable from SV40 have been detected in several types of human tumours. The oncoprotein of Simian virus 40, SV40 large T-antigen (Tag), is known to bind and inactivate tumour suppressor proteins, such as members of the retinoblastoma family and p53, thereby promot …

human alpha 1-antitrypsin promoter, human immunoglobulin heavy chain genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, Simian virus 40 poly A ...

Chang C, Simmons DT, Martin MA and Mora PT (1979) Identification and partial characterization of new antigens from simian virus 40-transformed mouse cells. J Virol 31: 463-471.. Crawford LV, Pim DC and Bulbrook RD (1982) Detection of antibodies against the cellular protein p53 in sera from patients with breast cancer. Int. J. Cancer 30: 403-408.. Crawford L (1983) The 53,000-dalton cellular protein and its role in transformation. Int. Rev. Exp. Path. 25: 1-50.. De Leo AB, Jay G, Appella E, Dubois GC, Law LW and Old LJ (1979) Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse. Proc Natl Acad Sci USA 76: 2420-2424.. Kress M, May E, Cassingena R and May P (1979) Simian Virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 serum. J. Virol. 31: 472-483.. Lane DP and Crawford LV (1979) T antigen is bound to a host protein in SV40-transformed cells. Nature 278: 261-263.. Linzer DIH and Levine AJ ...

Subramani, S. Rescue of chromosomal T-antigen sequences onto extrachromosomally replicating, defective simian virus 40 DNA by homologous recombination.. Molecular and Cellular Biology 6.4 (1986): 1320-1325. Web. 06 Aug. 2020. ...

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AppliedStemCell eCommerce Platform Patient Fibroblasts Immortalization Service [ASC-6020F] - Simian virus 40 (SV40) T antigen has been shown to be the simplest and most reliable agent for the immortalization of many different cell types and the mechanism

p53 is a cellular-encoded phosphoprotein first identified in protein complexes with the large tumor (T) antigen of simian virus 40 (SV40) (Linzer and Levine, 1979; Lane and Crawford, 1979). High...

A hybrid virus is a type of computer virus that combines elements of other virus programs in a new combination that will often...

Goat polyclonal antibody raised against synthetic peptide of V5 Tag. A synthetic peptide (conjugated with KLH) corresponding to amino acids 95-108 of RNA polymerase alpha of subunit simian virus 5. (PAB14287) - Products - Abnova

An article can be defined as the Most Viewed based on the number of clicks to the page, visits, downloads and sharing from the Journal of Clinical and Cellular Immunology .

A comparison of the proteins synthesized in human cells at late times after infection with adenovirus (Ad2) and with the adeno-simian virus 40 (SV40) hybrid viruses revealed polypeptides of 30,000 and 92,000 molecular weight specific for the hybrid viruses Ad2+ND1 and Ad2+ND4, respectively. Cell-free translation of SV40-specific mRNA, prepared from these cells by hybridization of total cytoplasmic RNA to SV40 DNA, showed that the mRNAs specifying these two polypeptides were at least partially encoded by the SV40 portion of the hybrid viruses. Cell-free translation of SV40-specific mRNA prepared from monkey cells infected with SV40 produced polypeptides of 40,000, 43,000, 48,500, and 92,000 molecular weight. The SV40 and Ad2+ND4 92,000-molecular-weight polypeptides made in vitro were very similar in electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels to the polypeptide precipitated by Tegtmeyer (1974) with SV40 anti-T serum.

Human polyomaviruses (JC virus, BK virus and simian virus 40) are causative agents of some human diseases and, interestingly, are involved in processes of cell transformation and oncogenesis. These viruses need the cell cycle machinery of the host cell to complete their replication; so they evolved mechanisms that can interfere with the growth control of infected cells and force them into DNA replication. The retinoblastoma family of proteins (pRb), which includes pRb/p105, p107 and pRb2/p130, acts as one of the most important regulators of the G1/S transition of the cell cycle. Rb proteins represent an important target for viral oncoproteins. Early viral T antigens can bind all members of the pRb family, promoting the activation of the E2F family of transcription factors, thus inducing the expression of genes required for the entry to the S phase. The interaction between early viral antigens and cell cycle regulators represents an important mechanism through which viruses deregulate cell cycle ...

Transport of α-aminoisobutyric acid and 2-deoxy-d-glucose in African green monkey kidney cells was measured 8 to 100 hr following permissive simian virus 40 infection. No differences in transport were detected during the time-period studies, and no significant differences were seen between the apparent Michaelis-Menten constants of normal and virally infected cells. The absence of transport enhancement in permissive simian virus 40 infection suggests that the augmented transport of viral-transformed cell lines devolves upon altered host genome function.. ...

Alpha-globin transcription factor CP2 is a protein that in humans is encoded by the TFCP2 gene.[1][2] TFCP2 is also called Late SV40 factor (LSF) and it is induced by well known oncogene AEG-1.[3] Late SV40 factor (LSF) also acts as an oncogene in hepatocellular carcinoma.[4] Late SV40 factor (LSF) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9).[5] Along with its main oncogene function in hepatocellular carcinoma (HCC) it plays multifaceted role in chemoresistance, epithelial-mesenchymal transition (EMT), allergic response, inflammation and Alzheimers disease.[4][6] The small molecule FQI1 (factor quinolinone inhibitor 1) prevents LSF from binding to HCC DNA which results in HCC cell death.[4][6][7] ...

COS are fibroblast-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 cells with a version of the SV40 virus that can produce large T antigen but has a defect in genomic replication. The CV-1 cell line in turn was derived from the kidney of the African green monkey. The acronym COS is derived from the cells being CV-1 (simian) in Origin, and carrying the SV40 genetic material. Two forms of COS cell lines commonly used are COS-1 and COS-7. The COS cell lines are often used by biologists when studying the monkey virus SV40. Cells from these lines are also often transfected to produce recombinant proteins for molecular biology, biochemistry, and cell biology experiments. When an expression construct with an SV40 promoter is introduced into COS cells, the vector can be replicated substantially by the large T antigen. These COS cells are genetically modified to produce the T antigen from their own genome. Jensen FC, Girardi AJ, Gilden RV, Koprowski H ...

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Because of the high homology that exists between the DNA of SV40 and the DNA of the JC or BK viruses, the identity of the band obtained by PCR and visualized by

A family of dihydroquinolinones that inhibited the proliferation of a number of cancer cell lines and targeted the oncogenic activities of the late simian virus 40 factor (LSF) was discovered. The lead quinolinone inhibitors, 8-(2-propoxyphenyl)-7,8-dihydro-[1,3]dioxolo[4,5-g]quinolin-6(5H)-one, FQI1, and 8-(2-propoxyphenyl)-[1,3]dioxolo[4,5-g]quinolin-6(5H)-one, FQI2, were determined by a comprehensive SAR study. The lead compounds had low micromolar to nanomolar Gi50S and IC50S (concentrations that induced 50% inhibition) in cell growth and LSF-directed luciferase reporter assays, respectively. A distinct correlation between the GI50 and IC50 values indicated antiproliferative effects resulted from inhibition of LSF activity. FQI1 had no growth effects on immortalized human hepatocytes or primary mouse hepatocytes. Overall, FQI1 proved a good drug candidate for hepatocellular carcinoma (HCC). It possessed a low molecular weight and moderate solubility, which was improved by substitution of the ...

mouse connexin45(Cx45) genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), mouse phosphoglycerate kinase promoter (PGK promoter), Phage P1 loxP, E. coli neo, E. coli ...

Simian Virus 40 or Simian vacuolating virus 40 - a polyomavirus that is found in both monkeys and humans. As with other polyomaviruses, it is a DNA virus that can cause tumors. ...

washed sheep red bloodand, hereby, warrant that these ingredients are safe for injection into the body of my patient. I have researched reports to the contrary, such as reports that mercury thimerosal causes severe neurological and immunological damage, and find that they are not credible.I am aware that some vaccines have been found to have been contaminated with Simian Virus 40 (SV 40) and that SV 40 is causally linked by some researchers to non-Hodgkins lymphoma and mesotheliomas in humans as well as in experimental animals. I hereby warrant that the vaccines I employ in my practice do not contain SV 40 or any other live viruses. (Alternately, I hereby warrant that said SV-40 virus or other viruses pose no substantive risk to my patient.)I hereby warrant that the vaccines I am recommending for the care of (Patients name) _______________ do not contain any tissue from aborted human babies (also known as fetuses ...

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Pk (V5) Epitope Tag (GKPIPNPLLGLDST), 1 mg. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).

Pk (V5) Epitope Tag (GKPIPNPLLGLDST), 0.1 mg. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).

Anderson HA, Chen Y, Norkin LC. 1998. MHC class I molecules are enriched in caveolae but do not enter with simian virus 40.. J Gen Virol. 79 ( Pt 6):1469-77. ...

Nuclear targeting sequences are essential for the transport of proteins into the nucleus. The seven-amino-acid nuclear targeting sequence of the SV40 large T antigen has been regarded as the model; however, many nuclear targeting sequences appear to be more complex. We suggest in this review that, d …

include ,EXTERN.h, #include ,perl.h, static PerlInterpreter *my_perl; /** my_eval_sv(code, error_check) ** kinda like eval_sv(), ** but we pop the return value off the stack **/ SV* my_eval_sv(SV *sv, I32 croak_on_error) { dSP; SV* retval; PUSHMARK(SP); eval_sv(sv, G_SCALAR); SPAGAIN; retval = POPs; PUTBACK; if (croak_on_error && SvTRUE(ERRSV)) croak(SvPVx_nolen(ERRSV)); return retval; } /** match(string, pattern) ** ** Used for matches in a scalar context. ** ** Returns 1 if the match was successful; 0 otherwise. **/ I32 match(SV *string, char *pattern) { SV *command = newSV(0), *retval; sv_setpvf(command, my $string = %s; $string =~ %s, SvPV_nolen(string), pattern); retval = my_eval_sv(command, TRUE); SvREFCNT_dec(command); return SvIV(retval); } /** substitute(string, pattern) ** ** Used for =~ operations that modify their left-hand side (s/// and tr///) ** ** Returns the number of successful matches, and ** modifies the input string if there were any. **/ I32 substitute(SV **string, char ...

include ,EXTERN.h, #include ,perl.h, static PerlInterpreter *my_perl; /** my_eval_sv(code, error_check) ** kinda like eval_sv(), ** but we pop the return value off the stack **/ SV* my_eval_sv(SV *sv, I32 croak_on_error) { dSP; SV* retval; STRLEN n_a; PUSHMARK(SP); eval_sv(sv, G_SCALAR); SPAGAIN; retval = POPs; PUTBACK; if (croak_on_error && SvTRUE(ERRSV)) croak(SvPVx(ERRSV, n_a)); return retval; } /** match(string, pattern) ** ** Used for matches in a scalar context. ** ** Returns 1 if the match was successful; 0 otherwise. **/ I32 match(SV *string, char *pattern) { SV *command = NEWSV(1099, 0), *retval; STRLEN n_a; sv_setpvf(command, my $string = %s; $string =~ %s, SvPV(string,n_a), pattern); retval = my_eval_sv(command, TRUE); SvREFCNT_dec(command); return SvIV(retval); } /** substitute(string, pattern) ** ** Used for =~ operations that modify their left-hand side (s/// and tr///) ** ** Returns the number of successful matches, and ** modifies the input string if there were any. **/ I32 ...

This module is the first in a series that discusses the discovery, structure, and function of DNA. Key experiments are discussed: from Griffiths discovery of genetic

Ive been reading a lot about CFIDs and fibro. Some theories are that is has a viral origin, EBV and HHV6 specifically.I have tested postive for both these viruses.....

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