TY - JOUR. T1 - A common signaling pathway via Syk and Lyn tyrosine kinases generated from capping of the sialomucins CD34 and CD43 in immature hematopoietic cells. AU - Tada, Jun Ichi. AU - Omine, Mitsuhiro. AU - Suda, Toshio. AU - Yamaguchi, Naoto. PY - 1999/6/1. Y1 - 1999/6/1. N2 - The sialomucin CD34 is a useful marker for hematopoietic stem/progenitor cells. However, the role of CD34 remains poorly understood. Here we investigate the functions of CD34 and another sialomucin CD43 coexpressed on hematopoietic stem/progenitor cells. Stimulation of undifferentiated hematopoietic KG1a cells with anti-CD34 or anti-CD43 induced homotypic cytoadhesion, accompanied by formation of a long-lived cap of CD34 and CD43 respectively, which colocalized with F-actin. Stimulation with either antibody specifically increased tyrosine phosphorylation of the identical set of proteins of Lyn, Syk, pp60, pp69, and pp77 at the capping site. These events were similar to those observed in monocytic U937 cells ...
Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then counterstained with alcian blue for half to one minute. There was a good correlation of results between the two methods. Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced into routine laboratories for the study of acid mucins in the gastrointestinal diseases.. ...
Hito Alcian Blue pH-2.5 OptimStain™ Kit is formulated for the staining of acid mucins, sulfated / carboxylated acid mucopolysaccharides and sulfated / carboxylated sialomucins (glycoproteins).
The Alcian Blue (pH 2.5) Stain Kit is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins ...
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15 0. We consider a one-sided test with the null hypothesis that, whatever the criterion management or detection sensitivity, speciВcity, or predictive value positive (PVP), the digitally acquired mam- mograms or lossy compressed IInj of a particular rate are worse than analog.
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Clone REA157 recognizes the TRA-1-60 antigen, a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, which is a transmembrane glycoprotein. Podocalyxin is a member of the CD34-related family of sialomucins and is recognized as a stem cell and tumor marker. TRA-1-60 antigen is expressed on the surface of human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonic carcinoma cells (EC). Expression of TRA-1-60 is lost upon differentiation and thus it can be used to assess the differentiation stage of the cells. TRA-1-60 can be detected in the serum of patients with germ cell tumors which contain an EC component. Additional information: Clone REA157 displays negligible binding to Fc receptors. - Liechtenstein
The goals of this study were to 1) demonstrate that the MTA rat mammary adenocarcinoma cell line is nonresponsive to treatment with the bisphosphonate risedronate and 2) compare the in vitro cellular responses of the MTA (nonresponder) and a related rat mammary adenocarcinoma cell line, MTB-01 (responder) to risedronate in order to identify factors responsible for the MTA cell lines response to bisphosphonate treatment.,To establish the MTA cell line as nonresponsive to risedronate treatment, Berlin- Druckery rats received an intracardiac injection of 105 MTA cells. Risedronate was administered daily to test animals beginning on the day of tumor cell inoculation. Control animals received MTA cells and daily saline injections. Animals were euthanized on day 14 and metastatic bone lesions were enumerated and area measurements were taken microscopically from femurs and vertebrae. There was no statistically significant difference in the size or number of metastatic foci in treated verses untreated ...
Bone is the most common site of metastasis for breast cancer, however the reasons for this remain unclear. We hypothesise that under certain conditions mammary cells possess osteomimetic capabilities that may allow them to adapt to, and flourish within, the bone microenvironment. Mammary cells are known to calcify within breast tissue and we have recently reported a novel in vitro model of mammary mineralization using murine mammary adenocarcinoma 4T1 cells. In this study, the osteomimetic properties of the mammary adenocarcinoma cell line and the conditions required to induce mineralization were characterized extensively. It was found that exogenous organic phosphate and inorganic phosphate induce mineralization in a dose dependent manner in 4T1 cells. Ascorbic acid and dexamethasone alone have no effect. 4T1 cells also show enhanced mineralization in response to bone morphogenetic protein 2 in the presence of phosphate supplemented media. The expression of several bone matrix proteins were monitored
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Biotin偶联MAdCAM1抗体[MECA-367](ab25021)可与小鼠样本反应并经WB, IP, IHC, AP, Flow Cyt, BL实验严格验证,被2篇文献引用,实验条件参看说明书。中国75%以上现货。
Little is known about the role of chromosomal abnormalities in the widely used models of rat mammary carcinogenesis. In this study, we cytogenetically analyzed nitrosomethylurea-induced rat mammary adenocarcinomas at different time points of development. As tools to study more advanced stages of malignant progression, we also analyzed the cytogenetic progression of tumors transplanted into younger syngeneic hosts, and of tumors that did not regress or that developed after host ovariectomy. Our results indicate that rat mammary adenocarcinomas appear to start development as diploid lesions with cytogenetically "normal" karyotypes. However, upon progression, tumors showed coexistence of normal diploid clones with abnormal clones bearing specific abnormalities affecting mainly chromosomes 1 and 15. Almost every ovary-independent tumor presented stem lines with specific nonrandom chromosomal abnormalities. Numerical chromosomal abnormalities such as specific trisomies started to develop mainly after ...
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Questionnaire: Tissue engeneering and regenerative medicine approaches are promising approaches to regenerate tissues damaged by trauma or diseases. Several cell types were evaluated based on their „biologic potential", of which cells from adipose tissue, easily harvested subcutaneously. The stromal vascular fraction (SVF) is a heterogeneous mixture of cells obtained by enzymatic digestion of human adipose tissue and depletion of mature adipocytes by centrifugation. It contains multipotent mesenchymal cells, referred to as adipose-derived stem/stromal cells (ASC), in higher numbers of clonogenic cells than the standardly-used bone marrow-derived cells, as well as hematopoietic and vascular cells. The sialomucin CD34 is a type-I transmembrane glycoprotein, the function of which is still largely unknown, broadly expressed by cells in SVF but rapidly lost by CD90+/CD34+ human ASC expanded in monolayer culture. We thus aimed at defining culture conditions allowing the maintenance of CD34 in ASC to ...
Wound Healing Assay Time series DIC images of mouse DA3 cells, derived from the mouse mammary adenocarcinoma cell line D1-DMBA-3, induced in BALB/...
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... is the most versatile and widely used technique for carbohydrate visualization. From a diagnostic point of view...
Invadopodia are actin-based membrane protrusions with high proteolytic activity that are thought to have an important role in cancer-cell invasion and intravasation. However, questions have remained about the formation and precise function of these structures during metastasis. On page 724, John Condeelis and colleagues extend their previous observation that neural Wiskott-Aldrich syndrome protein (N-WASP) is an essential component of invadopodia by discovering that N-WASP-mediated formation of invadopodia is required for the process of invasion and intravasation during breast cancer metastasis. Reducing the activity or expression of N-WASP in rat mammary adenocarcinoma cells leads to a reduction in the number and proteolytic activity of invadopodia in vitro, and decreases the invasive ability of the cells in vivo. The lack of functional N-WASP does not affect tumour size, but decreases the number of circulating tumour cells and the formation of metastasis. Primary tumour cells without ...
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Doubly cloned 13762NF rat mammary adenocarcinoma sublines of differing spontaneous metastatic potentials were tested for their response to ionizing radiation in vitro. Cells were irradiated using a 137Cs source, and colony formation was used to measure radiation survival. Heterogeneous responses of the various clones were observed and compared to clonal origin, spontaneous metastatic potential from mammary fat pad sites, and in vitro passage number. Clones MTF4 and MTF7, both derived from the locally growing tumor and possessing intermediate metastatic potentials, exhibited characteristic radiation survival curves (n = 8, Do = 89 rads, and n = 3, Do = 78 rads respectively). Clone MTA, also derived from the locally growing tumor but possessing high metastatic potential, had a more linear dose response to γ-irradiation (n = 1, Do = 143 rads, r = -0.99). Clone MTLn2, a lung metastases-derived clone which possesses a low metastatic potential, also exhibited a linear response (n = 1, Do = 175 rads, ...
Ive just returned from Copenhagen, Denmark, where I attended the 8th International Colloquium on Paratuberculosis (at considerable personal expense: Copenhagen is EXPENSIVE!) Current thinking among researchers in the field is that MAP itself is mostly not the culprit in the tissue damage. MAPs role is to initiate an inflammatory response in the bowel, through some unknown process/antigen/inflammatory-pathway. This immune response results in inflammation of bowel tissue, whereby the cells of the intestine physically separate, to allow immune cells, primarily macrophages and CD4 + CD8 T-cells, to reach the site of infection. This leads to "leaky gut" syndrome , which permits the contents of the bowel (most accurately described as being similar to the contents of a sewer), to leak through the bowel wall, resulting in a *massive* inflammatory response against the many microbes present in the fecal stream. Continue reading →. ...
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Myeloperoxidase (MPO) is an heterodimeric glycoprotein of 150 kDa with an α2/β2 structure. The two subunits (α and β) have a molecular weight of 55 and 15 kDa, respectively. MPO synthesis occurs in bone marrow at an early stage of myeloid lineage differentiation.
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18122-72-0 - XPMRNVZJQGDGFS-UHFFFAOYSA-I - Periodic acid (H5IO6), sodium salt (1:5) - Similar structures search, synonyms, formulas, resource links, and other chemical information.
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
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Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
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A PNAD Contínua visa produzir indicadores para acompanhar as flutuações trimestrais e a evolução, a médio e longo prazos, da força de trabalho e outras informações necessárias para o estudo e desenvolvimento socioeconômico do País ...