BACKGROUND: Podocalyxin-like 1 (PODXL) is a cell-adhesion glycoprotein and stem cell marker that has been associated with an aggressive tumour phenotype and poor prognosis in several forms of cancer. In this study, we investigated the prognostic impact of PODXL expression in colorectal cancer (CRC). METHODS: Using tissue microarrays and immunohistochemistry, PODXL expression was evaluated in 536 incident CRC cases from a prospective, population-based cohort study. Kaplan-Meier analysis and Cox proportional hazards modelling were used to assess the impact of PODXL expression on cancer-specific survival (CSS) and overall survival (OS). RESULTS: High PODXL expression was significantly associated with unfavourable clinicopathological characteristics, a shorter CSS (hazard ratio (HR) = 1.98; 95% confidence interval (CI) 1.38-2.84, P , 0.001) and 5-year OS (HR = 1.85; 95% CI 1.29-2.64, P = 0.001); the latter remaining significant in multivariate analysis (HR = 1.52; 95% CI 1.03-2.25, P = 0.036). In ...
The PODXL gene encodes for the podocalyxin-like protein 1 which is expressed in podocytes of the kidney glomeruli. PODXL is involved in the regulation of cell adhesion and cell morphology and functions to maintain the filtration pathway in glomeruli by anti-adhesive action on podocytes. PODXL has also been implicated in cancer progression by inducing cell migration and invasion. Based on its role in cancer, PODXL has been suggested as an emerging prognostic biomarker in various forms of cancer such as colorectal cancer ...
Purpose: : PODXL2 (also known as Endoglycan) is a protein closely related to CD34 and podocalyxin. Recent studies have implicated PODXL2 in leukocyte functions, while our own protein association assays have indicated potential interactions with retinal proteins involved in the development of Usher Syndrome. Little is currently known about this proteins role in healthy retinal function. This study seeks to determine the tissue expression pattern of PODXL2 and eludicate its role in retinal function and maintenance using genetic mouse models. Methods: : We developed mutant lines of mice with VelociGenes predesigned construct (#10133) from the KOMP. Expression of PODXL2 mRNA and protein was evaluated by RT-PCR, Immunoblotting, Immunofluorescence, and by following a lacZ reporter gene embedded in the gene targeting construct. Retinal function in the PODXl2 mice was assessed by ERG recordings. Results: : The knockout construct integration has been confirmed in PODXL2 -/- mice, with several regions ...
Definition of sialoglycoproteins in the Definitions.net dictionary. Meaning of sialoglycoproteins. What does sialoglycoproteins mean? Information and translations of sialoglycoproteins in the most comprehensive dictionary definitions resource on the web.
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Background: Podocalyxin-like 1 (PODXL) is an anti-adhesive transmembrane protein that has been demonstrated to be an independent factor of poor prognosis in colorectal cancer (CRC). The gene encoding PODXL is located to chromosome 7, which also harbours the gene for the epidermal growth factor receptor (EGFR). The aim of this study was to examine the associations between PODXL and EGFR expression in CRC in vitro and in vivo.. Methods: EGFR expression was analysed in tumours from three independent patient cohorts; cohort 1 (n = 533), cohort 2 (n = 259) and cohort 3 (n = 310), previously analysed for immunohistochemical PODXL expression and KRAS and BRAF mutations (cohort 1 and 3). Levels of EGFR and PODXL were determined by western blot in six different CRC cell lines.. Results: High expression of PODXL was significantly associated with high EGFR expression (p , 0.001) in all three cohorts, and with BRAF mutation (p , 0.001) in cohort 1 and 3. High EGFR expression correlated with BRAF mutation (p ...
Complete information for PODXL gene (Protein Coding), Podocalyxin Like, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
CHO-Anti-Human PODXL2 F(ab) stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human PODXL2 F(ab) gene to allow expression of the F(ab). It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Podocalyxin (gene name PODXL) is a CD34-related sialomucin implicated in the regulation of cell adhesion, migration and polarity. Upregulated expression of podocalyxin is linked to poor patient survival in epithelial cancers. However, it is not known if podocalyxin has a functional role in tumor progression. We silenced podocalyxin expression in the aggressive basal-like human (MDA-MB-231) and mouse (4T1) breast cancer cell lines and also overexpressed podocalyxin in the more benign human breast cancer cell line, MCF7. We evaluated how podocalyxin affects tumorsphere formation in vitro and compared the ability of podocalyxin-deficient and podocalyxin-replete cell lines to form tumors and metastasize using xenogenic or syngeneic transplant models in mice. Finally, in an effort to develop therapeutic treatments for systemic cancers, we generated a series of antihuman podocalyxin antibodies and screened these for their ability to inhibit tumor progression in xenografted mice. Although deletion of
SwePub titelinformation: Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer
Sialoglycoproteins: Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
PODXL兔多克隆抗体(ab104988)可与人样本反应并经WB, IHC, ICC/IF实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
In this study, we investigated the use of metabolic oligosaccharide engineering and bio-orthogonal ligation reactions combined with lectin microarray and mass spectrometry to analyze sialoglycoproteins in the SW1990 human pancreatic cancer line. Specifically, cells were treated with the azido N-acetylmannosamine analog, 1,3,4-Bu3ManNAz, to label sialoglycoproteins with azide-modified sialic acids. The metabolically labeled sialoglyproteins were then biotinylated via the Staudinger ligation, and sialoglycopeptides containing azido-sialic acid glycans were immobilized to a solid support. The peptides linked to metabolically labeled sialylated glycans were then released from sialoglycopeptides and analyzed by mass spectrometry; in parallel, the glycans from azido-sialoglycoproteins were characterized by lectin microarrays. This method identified 75 unique N-glycosite-containing peptides from 55 different metabolically labeled sialoglycoproteins of which 42 were previously linked to cancer in the literature
The authors describe a highly organized membrane-bound lumenal compartment that they name the "apicosome." They find that the apicosome is an intracellular perinuclear entity rich in apical proteins, positively expressing F-actin, EZRIN, and PODOCALYXIN (PODXL), among others. High accumulation of the marker PODXL has been identified in apicosomal-like structures of mouse blastocysts (Bedzhov et al., 2014). Taniguchi et al. (2017) also show that the apicosome is not present in mouse embryonic stem cells, which makes it a unique property of single-plated hPSCs and mouse epiblast cells. Its novelty is also highlighted by the fact that it does not costain with other organelle markers, making it an independent structure.. By the combination of ultrastructural analyses and live-cell imaging of isolated hPSCs expressing an EZRIN-GFP fusion protein, Taniguchi et al. (2017) characterize the apicosome as a membrane-bound lumen with a diameter of 3-5 µm. It is demarcated by a membrane containing highly ...
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PubMedID: 25396425 | Adhesive and migratory effects of phosphophoryn are modulated by flanking peptides of the integrin binding motif. | PloS one | 11/15/2014
ニワトリ・ポリクローナル抗体 ab26910 交差種: Hu 適用: WB…PODXL抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
Definition: D2-40 is a monoclonal antibody recognizing podoplanin , a 40 000 kD O-linked sialoglycoprotein located in lymphatic endothelial (...)
In a previous study, we demonstrated that nucleotides induce an increase in OPN mRNA steady-state levels in SMCs cultured in the presence or absence of serum.6 The present work shows that the UTP-induced OPN mRNA increase in rat SMCs results from both OPN mRNA stabilization and OPN gene transcription.. To date, few studies have tried to establish the mechanisms of OPN mRNA increase. In particular, it has been shown that neither transforming growth factor-β, a growth factor activating serine/threonine kinase receptors, nor intracellular receptor agonists, such as glucocorticoids and retinoic acid,37,38 induce OPN mRNA stability.3 In this study, we demonstrate that OPN mRNA level is regulated by UTP via a mRNA stabilization mechanism. Ang II, another agonist of G-protein-coupled receptors, can also stabilize OPN mRNA but not PDGF (data not shown). The sequence analysis of OPN mRNA reveals one potential sequence involved in mRNA stability in the 3′ UTR, TAAAT (located at 1084 to 1088). However, ...
Detect osteopontin protein with anti-osteopontin antibody products and ELISA kits for osteopontin test methods. Learn more about osteopontin protein.
Detect osteopontin protein with anti-osteopontin antibody products and ELISA kits for osteopontin test methods. Learn more about osteopontin protein.
R&D Systems™ Bovine Osteopontin/OPN Protein 50ug R&D Systems™ Bovine Osteopontin/OPN Protein Proteins O
Several studies indicate that local immunoregulation and associated cytokines have a putative role in the development of cancer. There is evidence that pro-inflammatory cytokines such as interleukin-1 (IL-1) are critically involved with tumour progression. IL-1 receptor antagonist (IL-1Ra) is known to down regulate and limit the inflammatory response. Therefore we attempted to examine the influence of the known polymorphism of the IL-1Ra gene on the development of human colorectal cancer (CRC). The study included 125 patients with CRC and 134 controls. Variable number tandem repeat (VNTR) polymorphism in intron 2 of the IL-Ra gene was analysed by the polymerase chain reaction method. There was a significant difference in genotype distribution between CRC patients and controls (P=0.025) and also in allelic frequencies (P=0.012). In detail the carriage rate of allele 3 in CRC patients was significantly increased compared with controls (P=0.007). We also found that the allelic distribution differs ...
Leukosialin also known as sialophorin or CD43 (cluster of differentiation 43) is a transmembrane cell surface protein that in humans is encoded by the SPN (sialophorin) gene. Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome. It also appears in about 25% of intestinal MALTomas.[citation needed] Using immunohistochemistry, CD43 can be demonstrated in the paracortical T-cells of healthy lymph nodes and tonsils; it is also positive in a range of lymphoid and myeloid tumours. Although it is present in over 90% of T-cell lymphomas, it is generally less effective at demonstrating this condition than is CD3 antigen. However, it may be useful as part of a panel to demonstrate B-cell ...
Sharp, Julie A., Sung, V., Slavin, J., Thompson, E. W. and Henderson, M. A. 1999, Tumor cells are the source of osteopontin and bone sialoprotein expression in human breast cancer, Laboratory investigation, vol. 79, no. 7, pp. 869-877. ...
Expression of PODXL (Gp200, PC, PCLP) in liver tissue. Antibody staining with HPA002110, HPA045507, CAB016169, CAB062558, CAB068219 and CAB068220 in immunohistochemistry.
Osteopontin has long been implicated in the process of carcinogenesis, progression, and metastatic dissemination of several human tumors, such as breast (12, 13), prostate (15), colon (16), ovarian (17), gastric (18), and lung (14) cancers and more recently in many other tumors, such as pancreatic, renal, endometrial, esophageal, and head and neck carcinomas (30).. In the present study, we decided to focus on osteopontin protein expression detected by immunohistochemistry in a large sample (207 cases) of NSCLCs, because these tumors still have a poor prognosis in spite of the notable advances in diagnosis, staging, treatment, and biological characterization. We analyzed the correlations between osteopontin expression and many clinicopathologic variables to clarify its possible prognostic role. As far as we know, our study is the largest retrospective analysis of the prognostic role of osteopontin expression in patients with NSCLC treated with curative surgery. Moreover, we decided to give ...
Quantikine® ELISA kit for human Osteopontin/OPN (Cat#SOST00). 0.024 ng/mL detection sensitivity. View Osteopontin/OPN ELISA kit details.
The design and selection criteria of the Framingham Offspring study have been described previously.2 On the basis of the sex-specific distributions of echocardiographic LV measurements, we sampled participants from the sixth examination cycle (1995-8) with both LV end diastolic dimension (LVEDD) and wall thickness (LVWT) below the sex-specific median (referent, n = 129), with increased LVEDD (⩾ 90th sex-specific centile, n = 134) and increased LVWT (⩾ 90th sex-specific centile, n = 128) in a 1:1:1 ratio. Eighteen participants were included in both LV dilatation and increased LVWT groups, so the study sample consisted of 373 participants. Participants underwent a standardised medical history and physical examination. Fasting blood samples were drawn and frozen at −70°C without any freeze-thaw cycles until assay. Plasma osteopontin was measured in duplicate by using an enzyme-linked immunosorbent assay (Calbiochem, Inc) with an intra-assay coefficient of variation of 4.6 ...
Chronic degenerative disease of the mares endometrium is characterised by changes in the uterine glands, including cystic dilation, hyperplasia and periglandular fibrosis. Endometrial biopsies were taken from 23 mares with different grades of endometrial degeneration. Solid structures were identified within the lumina of the uterine glands and shown to be calcified by histochemical staining. Most of them were not homogenous but composed of a mixture of mineral and organic substances. Further examinations of these mineralised structures by immunohistochemical methods revealed the presence of the non-collagenous matrix proteins osteopontin, osteonectin and bone sialoprotein, which are known to be involved in calcification processes such as urolithiosis. Osteopontin and bone sialoprotein were identified within the calculi, frequently arranged in concentric layers. Osteonectin was the only matrix protein that was also present in the glandular epithelium. Osteocalcin was not found in either the ...
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OPN (osteopontin), a pro-inflammatory cytokine, has recently emerged as a key factor in both vascular remodelling and the development of atherosclerosis. However, the relationship between OPN and atherosclerosis in patients without symptomatic cardiovascular disease is not clear. Therefore we measured plasma OPN levels and evaluated the correlation between plasma OPN levels and atherosclerosis as target organ damage in patients with EHT (essential hypertension). Plasma OPN levels were measured in 76 patients with EHT using a solid-phase sandwich ELISA. IMT (intima-media thickness), and Vd and Vs (mean diastolic and systolic flow velocities respectively) were evaluated by carotid ultrasound. The Vd/Vs ratio, an index of peripheral arterial resistance, was also calculated. The patients were divided on the basis of median OPN levels into a high-OPN group and a low-OPN group. The mean IMT and aldosterone levels were higher (P=0.024 and 0.031 respectively) and Vd/Vs was lower (P=0.007) in the ...
These data demonstrate icIL-1ra type I mRNA, sIL-1ra mRNA, and IL-1ra protein in human coronary arteries. IL-1ra protein colocalizes with IL-1β predominantly in the endothelium of these arteries, with less IL-1ra mRNA expression occurring in less-diseased DCM arteries. To our knowledge, this is the first documentation of IL-1ra expression by HCAECs. We also show only icIL-1ra type I mRNA expression in HUVECs and HCAECs stimulated in vitro with LPS/PMA or TGF-β. Previous reports have failed to detect IL-1ra mRNA in these cell types with the use of other stimuli and less sensitive detection methods, such as Northern blots,5 and there are no previous reports of immunohistochemistry for IL-1ra in human vessels. With the use of immunoprecipitation/Western blot techniques, our data also confirm that the expression of IL-1ra in HUVECs and HCAECs is intracellular. We have also demonstrated that the rare allele (IL-1RN *2/*2) of a VNTR polymorphism in the IL-1RN gene is associated with significantly ...
Recombinant Human IL-5RA protein is a HEK 293 Protein fragment 21 to 335 aa range, | 85% purity, | 1.000 Eu/µg endotoxin level and validated in SDS-PAGE.
Complete information for IL2RA gene (Protein Coding), Interleukin 2 Receptor Subunit Alpha, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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Mouse Monoclonal Anti-CD43/Sialophorin Antibody (W3/13) [DyLight 488]. Validated: Flow, IHC-Fr, IHC-P. Tested Reactivity: Rat. 100% Guaranteed.
article{9ffbdf28-43ac-4664-bbde-373cd7e125c5, abstract = {The use of cancer biomarkers to anticipate the outlines of disease has been an emerging issue, especially as cancer treatment has made such positive steps in the last few years. Progress in the development of consistent malignancy markers is imminent because advances in genomics and bioinformatics have allowed the examination of immense amounts of data. Osteopontin is a phosphorylated glycoprotein secreted by activated macrophages, leukocytes, and activated T lymphocytes, and is present in extracellular fluids, at sites of inflammation, and in the extracellular matrix of mineralized tissues. Several physiologic roles have been attributed to osteopontin, i.e., in inflammation and immune function, in mineralized tissues, in vascular tissue, and in kidney. Osteopontin interacts with a variety of cell surface receptors, including several integrins and CD44. Binding of osteopontin to these cell surface receptors stimulates cell adhesion, ...
Buy our Recombinant Human IL5RA protein (Fc Chimera). Ab83828 is a protein fragment produced in HEK 293 cells and has been validated in SDS-PAGE. Abcam…
Promab CD106 - This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different
Promab CD106 - This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different
XCL1 / Lymphotactin Protein LS-G5664 is a Recombinant Mouse XCL1 / Lymphotactin aa 22-114 produced in E. coli. It is biologically active.
Bone Sialoprotein antibody (integrin-binding sialoprotein) for ELISA, IHC-P, RIA, WB. Anti-Bone Sialoprotein pAb (GTX44333) is tested in Human samples. 100% Ab-Assurance.
Rabbit anti Human Lymphotactin antibody recognizes human Lymphotactin, otherwise known as XCL1, the only member of the C-chemokine family
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IRAP (Interleukin-1 Receptor Antagonist Protein) is an anti-inflammation therapy that is best for patients suffering from Osteoarthritis.
Laminins [(PUBMED:2404817)] are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation. They are composed of distinct but related alpha, beta and gamma chains. The three chains form a cross-shaped molecule that consist of a long arm and three short globular arms. The long arm consist of a coiled coil structure contributed by all three chains and cross-linked by interchain disulphide bonds. Beside different types of globular domains each subunit contains, in its first half, consecutive repeats of about 60 amino acids in length that include eight conserved cysteines [(PUBMED:2666164)]. The tertiary structure [(PUBMED:8648630), (PUBMED:8648631)] of this domain is remotely similar in its N-terminal to that of the EGF-like module. It is known as a LE or laminin-type EGF-like domain. The number of copies of the LE domain in the different forms of laminins is highly variable; from 3 up to 22 copies have been found. A schematic ...
Laminins [(PUBMED:2404817)] are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation. They are composed of distinct but related alpha, beta and gamma chains. The three chains form a cross-shaped molecule that consist of a long arm and three short globular arms. The long arm consist of a coiled coil structure contributed by all three chains and cross-linked by interchain disulphide bonds. Beside different types of globular domains each subunit contains, in its first half, consecutive repeats of about 60 amino acids in length that include eight conserved cysteines [(PUBMED:2666164)]. The tertiary structure [(PUBMED:8648630), (PUBMED:8648631)] of this domain is remotely similar in its N-terminal to that of the EGF-like module. It is known as a LE or laminin-type EGF-like domain. The number of copies of the LE domain in the different forms of laminins is highly variable; from 3 up to 22 copies have been found. A schematic ...
XCL1 protein is expressed in E. coli, processed, refolded and purified to yield the native, secreted form of the mature chemokine. XCL1 is a ligand for the G protein coupled receptor XCR1 with and EC50 ~ 50 nM. It has been shown the WT XCL1 is able to int
OPN3_MOUSE (Q9WUK7 ), OPN4A_DANRE (Q2KNE5 ), OPN4A_GADMO (Q804X9 ), OPN4B_GADMO (Q804Q2 ), OPN4B_XENLA (O57422 ), OPN4L_DANRE (Q1JPS6 ), OPN4_FELCA (Q5YKK9 ), OPN4_HUMAN (Q9UHM6 ), OPN4_MOUSE (Q9QXZ9 ), OPN4_PHOSU (Q5XXP2 ), OPN4_PODSI (Q4U4D2 ), OPN4_RAT (Q8R456 ), OPN4_RUTRU (Q6XL69 ), OPN5_HUMAN (Q6U736 ), OPN5_MOUSE (Q6VZZ7 ), OPS1_CALVI (P22269 ), OPS1_DROME (P06002 ), OPS1_DROPS (P28678 ), OPS1_MANSE (O02464 ), OPS1_SCHGR (Q94741 ), OPS2_DROME (P08099 ), OPS2_DROPS (P28679 ), OPS2_SCHGR (Q26495 ), OPS3_DROME (P04950 ), OPS3_DROPS (P28680 ), OPS4_DROME (P08255 ), OPS4_DROPS (P29404 ), OPS4_DROVI (P17646 ), OPS5_DROME (P91657 ), OPS6_DROME (O01668 ), OPSB_ANOCA (P51471 ), OPSB_APIME (P90680 ), OPSB_ASTFA (P51472 ), OPSB_BOVIN (P51490 ), OPSB_CARAU (P32310 ), OPSB_CHICK (P28682 ), OPSB_CONCO (O13227 ), OPSB_GEKGE (P35357 ), OPSB_HUMAN (P03999 ), OPSB_MOUSE (P51491 ), OPSB_ORYLA (P87365 ), OPSB_PANPA (P60573 ), OPSB_PANTR (P60015 ), OPSB_RAT (Q63652 ), OPSB_SAIBB (O13092 ), OPSC1_HEMSA (Q25157 ...