An IgG monoclonal antibody to group B meningococci cross-reacts with developmentally regulated polysialic acid units of...
The structurally similar polysialic acid capsules of group B meningococci and Escherichia coli K1 are poor immunogens, and attempts are currently being made to improve their immunogenicity by chemical modifications. An IgG monoclonal antibody to these polysialic acid capsules was used for the study of the presence of structurally similar components in tissue glycoproteins to investigate the reasons for the poor immunogenicity and to evaluate potential dangers in active or passive immunization. By immunoblotting polysialic acid was detected outside the brain in newborn rat kidney, heart, and muscle. It appeared in immunoblots as one component and with similar mobility to the neural cell adhesion molecule N-CAM. Specificity studies of the antibody and endosialidase treatment showed that the polysialic acid glycans detected were composed of chains as long as eight sialic acid residues or more. The polysialic acid was not detected in the corresponding tissues of the adult animal. These results ...
A sub-population of keratan sulphates derived from bovine articular cartilage is capped with α(2-6)-linked N-acetylneuraminic...
Alkaline borohydride-reduced keratan sulphate (KS) chains derived from bovine femoral head cartilage were fractionated by lectin affinity chromatography with Sambucus nigra agglutinin (SNA) into binding and non-binding populations. Analysis of the SNA-binding and non-binding KS chains using 600 MHz 1H n.m.r. spectroscopy showed that the former population contained alpha(2-6)-N-acetylneuraminic acid residues and the latter contained primarily alpha(2-3)-N-acetylneuraminic acid residues as chain terminators. Both populations contained a similar proportion of alpha(2-3)-N-acetylneuraminic acid residues within their protein-linkage regions, and similar sulphation and fucosylation levels. Analysis of these two fractions by gel-permeation chromatography (g.p.c.) on a TSK-30 XL column showed them to have the same size distributions. It was concluded from the n.m.r. spectra and g.p.c. data that the populations differed primarily in the mode of linkage of the chain-terminating sialic acids. ...
Total Synthesis of Legionaminic Acid as Basis for Serological Studies - Leibniz Institute for Natural Product Research and...
Matthies S, Stallforth P, Seeberger PH (2015) Total Synthesis of Legionaminic Acid as Basis for Serological Studies J Am Chem Soc 137, 2848-2851. ...
Adenovirus type 37 binds to cell surface sialic acid through a charge-dependent interaction
Most adenoviruses use the coxsackie-adenovirus receptor (CAR) as a major cellular receptor. We have shown recently that adenovirus types 8, 19a, and 37, which are the major causes of epidemic keratoconjunctivitis, use sialic acid rather than CAR as a major cellular receptor. The predicted isoelectric point of the receptor-interacting knob domain in the adenovirus fiber protein is unusually high (9.0-9.1) in type 8, 19a, and 37. The pKa of sialic acid is low, 2.6, implying a possible involvement of charge in fiber knob-sialic acid interactions. Here we show that (i) positively charged adenovirus knobs require sialic acid for efficient cell membrane interactions; (ii) viral and knob interactions with immobilized sialic acid or cell-surface sialic acid are sensitive to increased ionic strength; (iii) negatively charged molecules such as sulfated glycosaminoglycans inhibit the binding of virions to target cells in a nonspecific, charge-dependent manner; and that (iv) the ability of adenovirus knobs ...
A sub-population of keratan sulphates derived from bovine articular cartilage is capped with alpha(2-6)-linked N...
Alkaline borohydride-reduced keratan sulphate (KS) chains derived from bovine femoral head cartilage were fractionated by lectin affinity chromatography with Sambucus nigra agglutinin (SNA) into binding and non-binding populations. Analysis of the SNA-binding and non-binding KS chains using 600 MHz 1H n.m.r. spectroscopy showed that the former population contained alpha(2-6)-N-acetylneuraminic acid residues and the latter contained primarily alpha(2-3)-N-acetylneuraminic acid residues as chain terminators. Both populations contained a similar proportion of alpha(2-3)-N-acetylneuraminic acid residues within their protein-linkage regions, and similar sulphation and fucosylation levels. Analysis of these two fractions by gel-permeation chromatography (g.p.c.) on a TSK-30 XL column showed them to have the same size distributions. It was concluded from the n.m.r. spectra and g.p.c. data that the populations differed primarily in the mode of linkage of the chain-terminating sialic acids.. ...
Decreased surface sialic acid content is a sensitive indicator of muscle damage - Iwata - 2013 - Muscle & Nerve - Wiley Online...
Iwata, Y., Suzuki, O. and Wakabayashi, S. (2013), Decreased surface sialic acid content is a sensitive indicator of muscle damage. Muscle Nerve, 47: 372-378. doi: 10.1002/mus.23632 ...
Frontiers | Impact of structural aberrancy of polysialic acid and its synthetic enzyme ST8SIA2 in schizophrenia | Frontiers in...
Psychiatric disorders are a group of human diseases that impair higher cognitive functions. Whole-genomic analyses have recently identified susceptibility genes for several psychiatric disorders, including schizophrenia. Among the genes reported to be involved in psychiatric disorders, a gene encoding a polysialyltransferase involved in the biosynthesis of polysialic acid (polySia or PSA) on cell surfaces has attracted attention for its potential role in emotion, learning, memory, circadian rhythm, and behaviors. PolySia is a unique polymer that spatio-temporally modifies neural cell adhesion molecule (NCAM) and is predominantly found in embryonic brains, although it persists in areas of the adult brain where neural plasticity, remodeling of neural connections, or neural generation is ongoing, such as the hippocampus, subventricular zone, thalamus, prefrontal cortex, and amygdala. PolySia is thought to be involved in the regulation of cell-cell interactions; however, recent evidence suggests that it is
Sialic Acids Sweeten a Tumors Life | Cancer Research
Tumor cells of various origins feature increased expression of sialic acid sugars on membrane glycoproteins and glycolipids and their secretion into the tumor microenvironment. Sialic acids are synthesized in and expressed by essentially every vertebrate cell, and are involved in multiple different physiologic processes. However, hypersialylation of tumor cells relative to their untransformed normal counter parts specifically benefits tumor cell growth and correlates with a poor prognosis for patients with cancer (1, 2). Sialic acids comprise a family of more than 50 carbohydrates that share a nine-carbon backbone (C1-9) to which specific chemical modifications are enzymatically attached inside the cell. The most common sialic acid derivate found in mammals is N-Acetylneuraminic acid (Neu5Ac) which bears an acetyl group on the fifth carbon atom (C5). In general, sialic acids terminate the outer end of glycans (sialoglycans), where they are enzymatically linked to other carbohydrates, such as the ...
The Enigma of Enzymatic Sialic Acid O-Acetylation
View more ,Naturally occurring sialic acids can be O-acetylated at any one of four hydroxyl groups, located at position C-4, -7, -8, and -9. This modification, which is found in nearly all animals expressing sialic acids and certain bacteria, is known to be involved in regulating a variety of biological events. One of the more important processes that appear to be heavily influenced by O-acetylation is cancer development. The present review outlines some of the more recent advances towards understanding the role and regulation of sialic acid O-acetylation in human colorectal cancer, basalioma and melanoma. Even though great strides have been made towards identifying and characterising the biological role of O-acetylated sialic acids, detailed information concerning the transferase activity responsible for this modification remains vague. The 7(9)-O- and 4-O-specific acetyltransferases were identified in bovine and horse submandibular glands, respectively, over 30 years ago, however despite the ...
N-Acetyl-Neuraminic Acid | C11H19NO9 - PubChem
N-Acetyl-Neuraminic Acid | C11H19NO9 | CID 439197 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
Polysialic Acid, a Glycan with Highly Restricted Expression, Is Found on Human and Murine Leukocytes and Modulates Immune...
PolySia expression and function have been extensively studied in the nervous system, but the distribution and role of polySia in the immune compartment is still undefined. Herein we demonstrated the presence of polySia on human NK cells and its absence on other immune subsets, a finding that is in accord with two earlier reports (26, 27). NK cells up-regulated the expression of polySia and its scaffold NCAM upon activation by IL-2 treatment. This coregulation suggested that the heightened polySia signals were due to an up-regulation of both scaffold and glycan, rather than solely attributable to expanded polySia chain length. In support of this conclusion, a comparison of DP on resting and activated cells showed that upon activation total polySia was increased and the DP became more homogenous, tending toward small- to medium-length chains. To our knowledge, this is the first elucidation of polySia DP on primary human cells. The DP values we observed were slightly larger than analogous ...
Neuraminidase-dependent degradation of polysialic acid is required for the lamination of newly generated neurons<...
TY - JOUR. T1 - Neuraminidase-dependent degradation of polysialic acid is required for the lamination of newly generated neurons. AU - Sajo, Mari. AU - Sugiyama, Hiroki. AU - Yamamoto, Hideaki. AU - Tanii, Takashi. AU - Matsuki, Norio. AU - Ikegaya, Yuji. AU - Koyama, Ryuta. PY - 2016/1/5. Y1 - 2016/1/5. N2 - Hippocampal granule cells (GCs) are generated throughout the lifetime and are properly incorporated into the innermost region of the granule cell layer (GCL). Hypotheses for the well-regulated lamination of newly generated GCs suggest that polysialic acid (PSA) is present on the GC surface to modulate GC-to-GC interactions, regulating the process of GC migration; however, direct evidence of this involvement is lacking. We show that PSA facilitates the migration of newly generated GCs and that the activity of N-acetyl-α-neuraminidase 1 (NEU1, sialidase 1) cleaves PSA from immature GCs, terminating their migration in the innermost GCL. Developing a migration assay of immature GCs in vitro, ...
Polysialic acid blocks mononuclear phagocyte reactivity, inhibits complement activation, and protects from vascular damage in...
In line with our recent studies, demonstrating that interaction between sialic acid residues and SIGLEC11 contributes to decreased inflammation and neurotoxicity in vitro (Wang & Neumann, 2010; Shahraz et al, 2015), we now provide further insights into the in vivo effects of polysialic acid. SIGLEC11 and oligoSia and polySia were detected in the neuroretina of human post‐mortem donors. In comparison with the human retina, the murine retina showed a more speckled and more even distribution of sialic acids as reported before for rodent retinas (Bartsch et al, 1990; Sawaguchi et al, 1999).. We carefully assessed the immunomodulatory potential of polySia avDP20 in the laser coagulation mouse model in humanized transgenic mice expressing SIGLEC11 on microglia and tissue macrophages. Laser coagulation damage induces AMD‐relevant pathomechanisms including choroidal neovascularization preceded by Iba1‐positive cell accumulation at the RPE/choroid (Eter et al, 2008; Liu et al, 2013; Lückoff et al, ...
NA - Neuraminidase - Influenza A virus (A/swine/Missouri/2124514/2006(H2N3)) - NA gene & protein
Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and
Using sialic acid analogues to define the molecular basis of complement resistance mediated by sialylation of neisseria...
Using sialic acid analogues to define the molecular basis of complement resistance mediated by sialylation of neisseria gonorrhoeae lipopolysaccharide and to design novel therapeutics
Identification of amino acid residues at the active site of endosialidase that dissociate the polysialic acid binding and...
Endosialidase (endo-N-acetylneuraminidase) is a tailspike enzyme of bacteriophages specific for human pathogenic Escherichia coli K1, which specifically recognizes and degrades polySia (polysialic acid). polySia is also a polysaccharide of the capsules of other meningitis- and sepsis-causing bacteria, and a post-translational modification of the NCAM (neural cell-adhesion molecule). We have cloned and sequenced three spontaneously mutated endosialidases of the PK1A bacteriophage and one of the PK1E bacteriophage which display lost or residual enzyme activity but retain the binding activity to polySia. Single to triple amino acid substitutions were identified, and back-mutation constructs indicated that single substitutions accounted for only partial reduction of enzymic activity. A homology-based structural model of endosialidase revealed that all substituted amino acid residues localize to the active site of the enzyme. The results reveal the importance of non-catalytic amino acid residues for ...
Identification of amino acid residues at the active site of endosialidase that dissociate the polysialic acid binding and...
Endosialidase (endo-N-acetylneuraminidase) is a tailspike enzyme of bacteriophages specific for human pathogenic Escherichia coli K1, which specifically recognizes and degrades polySia (polysialic acid). polySia is also a polysaccharide of the capsules of other meningitis- and sepsis-causing bacteria, and a post-translational modification of the NCAM (neural cell-adhesion molecule). We have cloned and sequenced three spontaneously mutated endosialidases of the PK1A bacteriophage and one of the PK1E bacteriophage which display lost or residual enzyme activity but retain the binding activity to polySia. Single to triple amino acid substitutions were identified, and back-mutation constructs indicated that single substitutions accounted for only partial reduction of enzymic activity. A homology-based structural model of endosialidase revealed that all substituted amino acid residues localize to the active site of the enzyme. The results reveal the importance of non-catalytic amino acid residues for ...
Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus | mSphere
All cells are decorated on their surfaces with a complex array of oligosaccharides (glycans) that are attached to membrane glycoproteins and glycolipids or which make up the different components of soluble extracellular materials such as mucus and which are involved in the initial interactions of viruses or other pathogens with cells or mucosal surfaces (1). The chemical diversity of these glycans contributes to their complex functional roles in controlling cell-cell, cell-pathogen, and cell-environment interactions. Linear and branched glycoconjugates of vertebrate cells are often terminated in sialic acids (Sias). Sias have critical roles in maintaining a variety of cell functions due to their abundance as exposed terminal sugars, where they are involved in highly specific and regulated cellular lectin interactions (2, 3). They have also been exploited by many different pathogens as well as by nonpathogenic microbes for host recognition and attachment, including playing a role as receptors for ...
Sialic Acid Analysis | Life Sciences | SGS
Sialic Acid Analysis from SGS - a powerful technique to help you identify and quantify sialic acid species in your biopharmaceutical product.
CATH Domain 2ya5B01
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates ...
N-AcetyIneuraminic Acid, Sialic acid; NANA; Neu5Ac, 25mg - EY Laboratories, Inc.
[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/CXXXX/C-6008-25.pdf]N-AcetyIneuraminic Acid, Sialic acid; NANA; Neu5Ac, 25mg
NEW PSA on hormone therapy - Prostate Cancer - HealingWell.com Forum
Thanks everyone, so glad I come here. I will tell him about the lupron shot. My Uncle does not complain but we know he is very tired and he is losing the strength in his arms (he is a commercial builder). The doctor asked him if he was reading the Snuffy Myers book when he asked about Intermittent. I told him he is going to have to wait until he can approach that question again.. ...
ScamVictimsUnited.com • View topic - Looking for victims willing to be interviewed
I received and email from someone that is putting together a PSA on scams and is looking for scam victims to interview. If you fit the following criteria, please contact me so I can get you in touch with her as soon as possible ...
INT326367 - wiki-pain
In previous studies, we have shown that the highly sialylated renal anti-adhesin Podocalyxin (PC) is expressed in the developing brain [13], [14 ...
Polysialic acid-neural cell adhesion molecule in brain plasticity: from synapses to integration of new neurons. | Center for...
Abstract: Isoforms of the neuronal cell adhesion molecule (NCAM) carrying the linear homopolymer of alpha 2,8-linked sialic acid (polysialic acid, PSA) have emerged as particularly attractive candidates for promoting plasticity in the nervous system. The large negatively charged PSA chain of NCAM is postulated to be a spacer that reduces adhesion forces between cells allowing dynamic changes in membrane contacts. Accumulating evidence also suggests that PSA-NCAM-mediated interactions lead to activation of intracellular signaling cascades that are fundamental to the biological functions of the molecule. An important role of PSA-NCAM appears to be during development, when its expression level is high and where it contributes to the regulation of cell shape, growth or migration. However, PSA-NCAM does persist in adult brain structures such as the hippocampus that display a high degree of plasticity where it is involved in activity-induced synaptic plasticity. Recent advances in the field of ...
Elevated Serum Sialic Acid Concentration in NIDDM and Its Relationship to Blood Pressure and Retinopathy | Diabetes Care
Objective- In view of the possible link between serum sialic acid and cardiovascular disease in the general population, we investigated whether serum total and lipid-associated sialic concentrations are elevated in NIDDM patients compared with normal subjects. We also investigated how sialic acid levels relate to glycemic control, blood pressure, microalbuminuria, retinopathy, and serum lipid levels.. Research Design and Methods- We selected 20 NIDDM patients at random and matched them for age and sex with 20 normal subjects. The patients also had a similar BMI as the control subjects. A first morning blood sample was taken for sialic acid, glucose, fructosamine, and lipid analysis, as was a first morning urine sample for assessment of microalbuminuria. Retinopathy was assessed by fundoscopy.. Results- Both total and lipid-associated sialic acid levels were elevated in the NIDDM patients compared with control subjects (mean ± SD, total: 0.74 ± 0.11 vs. 0.60 ± 0.22 g/L, P , 0.02; ...
Fibrinogen-bound sialic acid levels in the dysfibrinogenaemia of liver disease | Meta
Fibrinogen-bound sialic acid levels were determined in 75 normal controls and 80 patients with liver disease. Patients with abnormal fibrin monomer polymerisation (FMP) had sialic acid levels significantly higher than controls or patients with normal FMP. Enzymatic removal of sialic acid from the abnormal fibrinogens corrected the abnormal FMP and thrombin-clotting times to the range of desialated controls. The accelerating effects of calcium ions, protamine sulphate and Polybrene were largely abolished by desialation, suggesting that these cations accelerate FMP by neutralising the negativity charged sialic acid residues. ...
1991 - Serum sialic acid predicts cardiovascular mortality | 1991 May-Jun : Volume 114, Number 3, Page 91 | ACP Journal Club...
Lindberg and colleagues report on a fascinating observation that serum sialic acid level predicts cardiovascular (and noncardiovascular) mortality. The studys classical design of measuring a factor and relating it to subsequent mortality was greatly strengthened by its large community-based sample. However, this study raises several concerns. First, it is unclear whether any hypothesis about sialic acid had been set out by the investigators 20 years ago or whether this was part of the series of protein measurements that was done without a particular hypothesis. Second, because the measurement of sialic acid was done in the 1960s, another sialated protein may have been measured. Third, many more risk factors for coronary disease are known now than were known 20 years ago; for example, the only lipid measured in that study was total cholesterol. Thus, the relation of other lipids and lipoproteins to sialic acid could not be determined. Also, no data on cigarette smoking were provided. Fourth, ...
Removal of sialic acid from cardiac sarcolemma does not affect contractile function in electrically stimulated guinea pig left...
Calcium is essential for the maintenance of contraction in the heart, and it has been suggested that the glycoprotein matrix on the external surface of cardiac cells is a critical factor in the supply of activator calcium to the beating heart. Sialic acid residues are important calcium-binding sites in the matrix and treatment with neuraminidase, an enzyme which cleaves sialic acid from oligosaccharide chains, has been reported to abolish spontaneous contraction in cultured heart cells, without causing general breakdown in the plasma membrane. However, we report here that in intact, electrically stimulated guinea pig atrial preparations, neuraminidase treatment produces no significant changes in resting tension or force of contraction. It is also without effect on the response to calcium removal and replacement, and inotropic or toxic concentrations of cardiac glycosides. In these experiments, up to 79% of the total tissue sialic acid was removed, and electron microscopy studies showed that this
Localization of the putative sialic acid-binding site on the immunoglobulin superfamily cell-surface molecule CD22. - Oxford...
B-lymphocyte antigen CD22 is a member of the recently described sialoadhesin family of immunoglobulin-like cell-surface glycoproteins that bind glycoconjugates terminating in sialic acid. One prominent ligand for CD22 is the highly glycosylated leukocyte surface protein CD45. Using surface plasmon resonance spectroscopy, we characterized the interaction of recombinant mouse CD22 with native CD45 purified from rat thymus (CD45-thy). By in situ desialylation and resialylation of immobilized CD45-thy, we show that mouse CD22 binds to the sialoglycoconjugate NeuGc alpha 2-6Gal beta 1-4GlcNAc carried on CD45-thy N-glycans. Previous studies have shown that the sialic acid-binding site lies within the two membrane-distal domains of CD22 (domains 1 and 2), which are V-set and C2-set immunoglobulin superfamily domains, respectively. To further localize the binding site, we have made 42 single amino acid substitutions throughout both domains. All 12 mutations that abrogated binding to CD45-thy without disrupting
Polysialic acid transport protein elisa and antibody
Shop Polysialic acid transport protein ELISA Kit, Recombinant Protein and Polysialic acid transport protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Sialic acid levels in the blood in pregnant women with impaired glucose tolerance test | AVESİS
Purpose Gestational diabetes (GDM) is a metabolic disease characterized by the impairment of glucose tolerance during pregnancy. Sialic acid (SA) is a component of glycolipid and glycoproteins found in hormone and enzymes in serum and tissues and high serum SA levels are observed in diabetic patients. ...
Universitätsklinikum Halle(Saale): Project SP2
We have generated two transgenic mice strains (one is overexpressing the mutated key enzyme of the sialic acid biosynthesis, which leads to high sialic acid levels and one has a defect in the sialic acid biosynthesis). Both strains will be compared with wild-type animals. We plan to analyse O-GlcNAc, sialic acid, sialic acid binding proteins and sialic acid-dependent differentiation markers in all organs over the whole lifespan and quantify age-dependent muscle performance in vivo. The outcome of metabolic sialic acid engineering will be analysed in embryonic stem cells (differentiation) and neuronal cells (neurite outgrowth e.g. regeneration). We also plan to analyse the involvement of sialylation and O-GlcNAcylation on the function of endothelial cells. After metabolic engineering we will analyse their barrier capacity and age-related impact on neuronal cells by real-time cell analysis. Since high levels of glucose induce glycation of proteins, we will analyse the function of an artificial ...
Universitätsklinikum Halle(Saale): Project SP2
We have generated two transgenic mice strains (one is overexpressing the mutated key enzyme of the sialic acid biosynthesis, which leads to high sialic acid levels and one has a defect in the sialic acid biosynthesis). Both strains will be compared with wild-type animals. We plan to analyse O-GlcNAc, sialic acid, sialic acid binding proteins and sialic acid-dependent differentiation markers in all organs over the whole lifespan and quantify age-dependent muscle performance in vivo. The outcome of metabolic sialic acid engineering will be analysed in embryonic stem cells (differentiation) and neuronal cells (neurite outgrowth e.g. regeneration). We also plan to analyse the involvement of sialylation and O-GlcNAcylation on the function of endothelial cells. After metabolic engineering we will analyse their barrier capacity and age-related impact on neuronal cells by real-time cell analysis. Since high levels of glucose induce glycation of proteins, we will analyse the function of an artificial ...
Sialic acids are essential molecule with high structural diversity - GLYX-13 microinjection produced a long-lasting...
Sialic acids are essential molecule with high structural diversity. metastasis and growth, microbiome biology, and pathology. It allows molecular mimicry in pathogens which allows them to flee sponsor immune responses. Sialic acidity offers discovered part in therapeutics Recently. In this section weve highlighted the (i) variety of sialic acidity, (ii) their event in the varied existence forms, (iii) sialylation and disease, and (iv) sialic acidity and therapeutics. (((((((pathogen C [78] and murine hepatitis S pathogen [81], respectively. 3.1. acetyltransferases can be found: (a) acetyl-CoA:sialate 7 [9]-((((((NeuD can be thought to enter the standard PSA biosynthetic pathway via NeuA/NeuS [164]; light-green discontinuous arrow: LPS can be exposed for the external membrane. Asterisk: LPS sialyltransferase; NanC, Neu5Ac-specific porin; Kps, PSA capsule export program; SatABCD, Neu5Ac ABC (ATP-binding cassette) transporter; SiaPQM, Neu5Ac Capture (tripartite ATP-independent periplasmic) ...
Allomyrina dichotoma - EY Laboratories, Inc.
The hemolymph of the Japanese beetle, Allomyrina dichotoma, contains a lectin with a specificity for β-D-galactose 1 . This lectin, which can be purified by affinity and ion-exchange chromatography, is composed of two isolectins, Allo A-I and Allo A-II. The material provided by EY Laboratories is a mixture of the two isolectins, with Allo A-II being the predominant isolectin. Allo A is active between pH 5-9. Allo A has a greater binding affinity for β-D-galactose than RCA-I and it does not bind to α-D-galactose 2. Immobilized Allo A-II binds most strongly with α2,6 sialylated complex-type oligosaccharides, but free N-acetylneuraminic acid up to 2M does not displace such glycoconjugates, nor does α2,3-link sialic acid react with the lectin 3 . In spleen cell activation studies the Allo A lectin has been shown to be as mitogenic as Con A and more mitogenic than PHA-L. In the production of Interleukin-2 (Il-2), at a concentration of 0.5 µg/ml, Allo A had higher Il-2 units production than Con ...
Content 17-3
Variations of serum sialic acid level in liver cirrhosis. Saatea Arif, Najeeb-ul-Haq, Ruhila Hanif, A. Sattar Khan, Jamil-ur-Rehman, Taufeeq Ahmed Mufti. Comparison of students performance under composite scheme and split scheme of first professional MBBS. examination. Tehseen Iqbal, Naila Hamid, Hamid Javaid ...
Frontiers | Artemin and an Artemin-Derived Peptide, Artefin, Induce Neuronal Survival, and Differentiation Through Ret and NCAM...
Artemin (ARTN) is a neurotrophic factor from the GDNF family ligands (GFLs) that is involved in development of the nervous system and neuronal differentiation and survival. ARTN signals through a complex receptor system consisting of the RET receptor tyrosine kinase and a glycosylphosphatidylinositol-anchored co-receptor GFL receptor α, GFRα3. We found that ARTN binds directly to neural cell adhesion molecule (NCAM) and that ARTN-induced neuritogenesis requires NCAM expression and activation of NCAM-associated signaling partners, thus corroborating that NCAM is an alternative receptor for ARTN. We designed a small peptide, artefin, that could interact with GFRα3 and demonstrated that this peptide agonist induces RET phosphorylation and mimics the biological functions of ARTN - neuroprotection and neurite outgrowth. Moreover, artefin mimicked the binding of ARTN to NCAM and required NCAM expression and activation for its neurite elongation effect, thereby suggesting that artefin represents a binding
Plus it
In human serum, at least two molecular species of the neural cell adhesion molecule (NCAM) with molecular weights of 110,000-130,000 and 150,000-180,000, respectively, can be identified by Western blotting. Both are characterized by the absence of epitopes for monoclonal antibodies KD11 and MG5, which specifically recognize intracellular domains of the human NCAM transmembrane isoforms, NCAM-140 and NCAM-180. In contrast to the Mr 110,000-130,000 molecule also detectable in serum samples from healthy blood donors, the Mr 150,000-180,000 molecule appears to be tumor associated. The only difference between these two species is shown to be the presence of long chains of α-(2,8)-linked N-acetylneuraminic acids, which are characteristic for the so-called embryonic NCAM form. After treatment with endoneuraminidase N, the Mr 150,000-180,000 molecule can no longer be discriminated from the Mr 110,000-130,000 molecule in Western blotting as well as gel and anion exchange chromatography experiments. The ...
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Langley, K. und Gratzl, Manfred (1991): Neural Cells adhesion Molecule NCAM in neural and endocrine cells. In: Gratzl, Manfred und Langley, K. (Hrsg.): Markers for Neural and Endocrine Cells : molecular and cell biology, diagnostic applications. Winheim: Wiley-VCH Verlag. S. 133-178 [PDF, 6MB] ...
Publications of Faculty of Medicine: Significance Of Serum Lipid Peroxides, Total And Lipid-Bound Sialic Acid As Markers For...
Paper: Significance Of Serum Lipid Peroxides, Total And
Lipid-Bound Sialic Acid As Markers For Liver
Diseases , Author: Thanaa Hamed, Mahasen Abdel Sattar, Fauna Abd El- Salam*,
Sherif Abou El Dahab**, and Amal Idris , Year: 2000 , Faculty of Medicine, Benha University
Sialic acid synthase elisa and antibody
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sialic acid binding Ig like lectin 11 Biomolecules | Biocompare
Compare sialic acid binding Ig like lectin 11 Biomolecules from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
Most recent papers with the keyword Central nervous system recovery | Read by QxMD
Escherichia coli K1 strains are major causative agents of invasive disease of the new born. The age dependency of infection can be reproduced in the neonatal rat. Colonization of the small intestine following oral administration of K1 bacteria leads rapidly to invasion of the blood circulation; bacteria that avoid capture by the mesenteric lymphatic system and evade antibacterial mechanisms in the blood may disseminate to cause organ-specific infections such as meningitis. Some E. coli K1 surface constituents, in particular the polysialic acid capsule, are known to contribute to invasive potential but a comprehensive picture of the factors that determine the fully virulent phenotype has not so far emerged ...
Most recent papers with the keyword Neonatal diseases | Read by QxMD
Escherichia coli K1 strains are major causative agents of invasive disease of the new born. The age dependency of infection can be reproduced in the neonatal rat. Colonization of the small intestine following oral administration of K1 bacteria leads rapidly to invasion of the blood circulation; bacteria that avoid capture by the mesenteric lymphatic system and evade antibacterial mechanisms in the blood may disseminate to cause organ-specific infections such as meningitis. Some E. coli K1 surface constituents, in particular the polysialic acid capsule, are known to contribute to invasive potential but a comprehensive picture of the factors that determine the fully virulent phenotype has not so far emerged ...
Sialic Acids Summary Report | CureHunter
Sialic Acids: A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
Sialic Acids as Biomarkers for Cardiovascular Disease | EUROCARB 2019
Cardiovascular disease (CVD) is the leading cause of death for men and women worldwide, causing 3.9 million (45% of all deaths) each year in Europe alone [1]. CVD encompasses a wide array of diseases such as heart attack, stroke and coronary artery disease. Assessing CVD risk can be complex, due to the multitude of factors to consider. Taken together, these factors form risk assessments such as QRISK, giving a percentage chance of mortality in a given time frame [2]. While QRISK gives good insight into broad CVD risk factors, it may not be entirely accurate [3]. Therefore, biomarkers that could be used to improve the prediction of CVD mortality are of interest. These would not replace assessments such as QRISK but rather be used in conjunction for more accurate risk management. Sialic acid (Neu5Ac) has previously been identified as a biomarker for CVD [4]. Neu5Ac content can be affected by several other factors separate from CVD, as such five di-O-acetylated derivatives of Neu5Ac have been ...
Evolutionary gene mutation blamed for diabetes, obesity | EmaxHealth
Diabetes is estimated to affect over 25 million individuals in the U.S., and 285 million people worldwide, said Jane J. Kim, M.D., a -participating researcher from the Department of Pediatrics at the University of California, San Diego in La Jolla, CA. Our study for the first time links human-specific sialic acid changes to insulin and glucose metabolism and therefore opens up a new perspective in understanding the causes of diabetes.. The researchers studied two groups of mice that lacked CMAH. They found they could not produce a type of sialic acid called NeuSGc. The second group of mice were normal. When the mice were given a high fat diet, leading them to become obese. Only the mice lacking the ability to produce sialic acid on the cell surface from absence of NeuSGc developed impaired insulin production.. Gerald Weissmann, M.D., Editor-in-Chief of the FASEB Journal wonders if humans lack the CMAH enzyme for a reason related to survival. Perhaps it is a defense against bacteria or ...
Subject: sialic acids / Publication Year: 2017 - PubAg Search Results
In N-acetylneuraminic acid, apart from O9 and O8, a possible glycosylation site is the O4 position. For example, gangliosides HLG-2 and HPG-7 are considered to be potential lead compounds for carbohydrate-based drug development to treat neural disorders. However, the construction of their α(1 → 4) fucosyl sialic acid and α(2 → 4) linkages between sialic acids is difficult because of the regioselec ...
Sialoglycoproteins Summary Report | CureHunter
Sialoglycoproteins: Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
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