TY - JOUR. T1 - Action of bovine serum albumin on cytochrome c oxidase activity and proton pumping. T2 - A role for fatty acids in enzyme function?. AU - Sharpe, Martyn. AU - Perin, Ivano. AU - Nicholls, Peter. PY - 1996/8/5. Y1 - 1996/8/5. N2 - Bovine serum albumin (BSA) at micromolar concentrations causes a red shift of the Sorer band of bovine cytochrome c oxidase with a slow biphasic time course. It also inhibits the turnover of detergent-isolated enzyme in a similarly slow manner; the progress of this inhibition is halted by palmitate and other fatty acids. The inhibitory bovine serum albumin effect may involve fatty acid depletion from the enzyme. Respiration by cytochrome c oxidase vesicles (proteoliposomes) in the presence of ionophores (uncontrolled) shows only a small inhibition by BSA but preincubation of such vesicles with BSA induces a loss of proton pumping activity. After incubation of BSA-depleted proteoliposomes in the presence of reductant with combinations of fatty acids, ...
Bovine serum albumin (BSA) protein is widely used to fabricate antifouling coatings for nanobiotechnology applications. Numerous BSA protein options are…
Rabbit Anti-Bovine Serum Albumin (BSA) IgG ELISA Kit, 96 tests, Quantitative ELISA Kit 710-110-BSR Rabbit Anti-Bovine Serum Albumin (BSA) IgG ELISA Kit, 96 tests, Quantitative ELISA Kit 710-110-BSR
Could anybody tell me please what happens to serum albumin on platinum Surfaces. More precisely : How can the unusual adsorption behaviour of serum albumin on platinum, measured by means of ellipsometry, be explained. The time dependence of the amount adsorbed is not reproducible. Thanks a lot ! Trixi Leistner, Joachim Steiner ...
The influence of electrostatic interactions on membrane fouling during the separation of bovine serum albumin (BSA) from solution was studied in a cross-flow ultratiltration system. Experiments were carried out at different pH values between 3.78 and 7.46; and for different ionic strengths between 0.001 M and 0.1 M. The changes in permeate flux, cake layer resistance, zeta potentials of BSA and polyether sulfone (PES) membranes, and electrostatic interaction energies, were evaluated. At all of the ionic conditions studied, PES membranes are negatively charged. However, BSA molecules are either negatively or positively charged depending on the ionic environment. Whereas the cake layer resistance decreased with increasing pH and ionic strength, the permeate fluxes increased. The calculated electrostatic energy was a minimum at the isoelectric point (IEP) of BSA. However, at this point, the cake resistances corresponding to fouling at each ionic strength, were not minimized. Below the IEP of BSA, ...
Zirconia is known to be one of the best materials for the chromatographic support due to its excellent chemical, thermal and mechanical stability. In this work we report preparation and use of bovine...
OBJECTIVE - To evaluate the prevalence of IgG antibodies to bovine serum albumin (BSA) in a cohort of Brazilian children and young adults with IDDM.RESEARCH DESIGN END METHODS - Sera from 81 subjects with ,1 year of IDDM (group 1), 111 subjects with ,1 year of IDDM (group 2), and 207 normoglycemic subjects were tested using an immunofluorimetric assay. A receiver-operating-characteristic curve was used to establish the threshold of anti-BSA antibody titers defining the positivity of the assay.RESULTS - the distribution of the fluorimetric index (FI) of anti-BSA antibodies did not have a gaussian profile. Rank sum of FI was significantly higher inpatients than in control subjects (P , 0.0001). Average logFI values of both IDDM groups were significantly higher than that of the control group (P , 0.005 for both groups). There was a trend toward higher FI levels in group 1 than in group 2 (P = 0.06). A FI cutoff of 0.7 optimized the ratio of true-positive to false-positive of the assay, with the ...
Rabbit recombinant monoclonal Bovine Serum Albumin antibody [EPR12774] - BSA and Azide free. Validated in WB, IHC and tested in Mouse, Rat, Cow, Human.
TY - JOUR. T1 - Epi-Fluorescence imaging at the air-water interface of fibrillization of bovine serum albumin and human insulin. AU - Sessions, Kristen. AU - Sacks, Stuart. AU - Li, Shanghao. AU - Leblanc, Roger M.. PY - 2014/7/15. Y1 - 2014/7/15. N2 - Protein fibrillization is associated with many devastating neurodegenerative diseases. This process has been studied using spectroscopic and microscopic methods. In this study, epi-fluorescence at the air-water interface was developed as an innovative technique for observing fibrillization of bovine serum albumin and human insulin. This journal is. AB - Protein fibrillization is associated with many devastating neurodegenerative diseases. This process has been studied using spectroscopic and microscopic methods. In this study, epi-fluorescence at the air-water interface was developed as an innovative technique for observing fibrillization of bovine serum albumin and human insulin. This journal is. UR - ...
Bovine Bovine Serum Albumin, FITC conjugated/tagged from Biomeda Corporation,FITC~Bovine Serum Albumin,biological,biology supply,biology supplies,biology product
Lab Reagents Bsa Assay Laboratories manufactures the typical bsa solution reagents distributed by Genprice. The Typical Bsa Solution reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact bsa assay. Other Typical products are available in stock. Specificity: Typical Category: Bsa Group: Solution Solution information ...
Description: A polyclonal antibody for detection of Bovine Serum Albumin from Cow. This Bovine Serum Albumin antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant ...
Crespo-Félez, I, Castañeda-Sampedro, A, Sánchez, DI, Fernández-Alegre, E, Álvarez-Rodríguez, M, Domínguez, JC et al.. Effect of Single Layer Centrifugation Porcicoll (70%, 80% and 90%) or supplementation with reduced glutathione, seminal plasma and bovine serum albumin on frozen-thawed boar sperm. Anim. Reprod. Sci. 2017;187 :167-173. doi: 10.1016/j.anireprosci.2017.11.002. PubMed PMID:29113727 ...
8. After washing extensively, place the column of Glutathione-Agarose beads in the Reaction Buffer containing 100 μg/ml Bovine Serum Albumin on ice (or at 4°C, see Hint #5 ...
Bovine plasma was collected at a slaughterhouse into 10% sodium citrate. Each experiment was performed with 2,000 ml of this plasma and pH was adjusted to 7.2 with acetate buffer, pH 4.0 (4 M sodium acetate and 10 M acetic acid). A mixture of ethanol and chloroform was slowly added under homogenization to a final concentration of 19% ethanol and 0.6% chloroform. This mixture was kept at 4oC and was homogenized for an additional 20 min in order to obtain complete hemoglobin precipitation. After this step, plasma was kept in a cold room at -5oC overnight for g-globulin precipitation. The precipitate consisting of hemoglobin and g-globulin was removed by centrifugation at 2,500 g for 10 min at -5oC (Sorvall RC-3B plus, Du Pont, Wilmington, DE, USA) and discarded. The pH of supernatant I (Figure 1) was adjusted to 6.0 with acetate buffer solution, pH 4.0, under homogenization for about 30 min at -5oC and the preparation was left to stand for 6 h to precipitate a- and ß-globulin, which were removed ...
The interactions of the sugars glucose and lactose with the transport protein bovine serum albumin (BSA) were investigated using fluorescence, FT-IR and circular dichroism (CD) techniques. The results indicated that glucose could be bonded and transported by BSA, mainly involving hydrogen bonds and van der W
Bovine Serum Albumin - Lyophilized, Fraction V- Test Specification Protein Purity (HPLC) Not less than 98% Sodium, 10% Solution Not More Than 50 mEq/L
This study investigated the interaction between eupatorin and bovine serum albumin (BSA) using ultraviolet-visible (UV-vis) absorption, fluorescence, synchronous fluorescence, circular dichroism (CD) spectroscopies, and molecular modeling at pH 7.4. Results of UV-vis and fluorescence spectroscopies illustrated that BSA fluorescence was quenched by eupatorin via a static quenching mechanism. Thermodynamic parameters revealed that hydrophobic and electrostatic interactions played major roles in the interaction. Moreover, the efficiency of energy transfer, and the distance between BSA and acceptor eupatorin, were calculated. The effects of eupatorin on the BSA conformation were analyzed using UV-vis, CD, and synchronous fluorescence. Finally, the binding of eupatorin to BSA was modeled using the molecular docking method.
合成系列含缩醛的双链正离子类脂分子,并用荧光光谱研究其与牛血清蛋白(BSA)的相互作用.通过荧光的变化,解释蛋白质构象的变化.在低类脂浓度时,少量类脂分子束缚在牛血清蛋白周围,荧光有很大幅度的淬灭,蛋白质本身肽链被解开,与此同时最大发射波长从(344±1) nm 蓝移到(331±1) nm.由于疏水相互作用,更多类脂分子不断地聚集在蛋白质周围,牛血清蛋白中的两个色氨酸残基被完全地包裹在类脂分子形成的双分子膜中,荧光强度不断增加直到恒定不变.;Interactions of a series of dialkyl cationic lipids linking with bovine serum albumin (BSA) through acetal (linker) have been studied by the fluorescence spectroscopy. At low concentrations of cationic lipids, the fluorescence intensity of BSA decreased with binding of cationic lipid, and the maximum of emission wavelength shifted from (344±1)nm to (331±1)nm. It indicates that the BSA goes to uncoiled flexible
Materials : Bovine Serum Albumin (MW 66.5 KDa, Faction V), Dipentaerythritol pentaacrylate (5Acl, MW 524.21), Propylamine, Pentylamine, Hexylamine,
BioAssay record AID 750838 submitted by ChEMBL: Binding affinity to bovine serum albumin assessed as decrease in fluorescence intensity by Scatchard plot analysis.
Concentration: 1 mg / ml, determined by the Lowry method Source: Bovine Serum Albumin was purchased from Boehringer Mannheim and was modified by carbamylation to form CBL modified BSA. Buffer: In 10 mM PBS, 0.15 M NaCl 0.5 mM EDTA, 0.01 % NaN3, pH 7.4. Storage: -20°C for short and long-term storage. Aliquot to avoid re
TY - JOUR. T1 - Hepatic disposition characteristics of glycosylated bovine serum albumin in rats. AU - Ogawara, K.. AU - Nishikawa, M.. AU - Takakura, Y.. AU - Hashida, M.. PY - 1996/1/1. Y1 - 1996/1/1. UR - http://www.scopus.com/inward/record.url?scp=0029790951&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0029790951&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0029790951. SP - 665. EP - 666. JO - Proceedings of the Controlled Release Society. JF - Proceedings of the Controlled Release Society. SN - 1022-0178. IS - 23. ER - ...
Sigma-Aldrich offers Sigma-A2153, Bovine Serum Albumin for your research needs. Find product specific information including CAS, MSDS, protocols and references.
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[153 Pages Report] Bovine Serum Albumin Market by Form, Grade (Technical-& Diagnostic-Grade, Research/Reagent-Grade, Food Grade), End User (Life Sciences Industry, Feed Industry, Food Industry, Research Institutes), and Region
Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
Global fetal bovine serum market is expected to grow at a significant CAGR in the upcoming period as the scope and its applications are rising enormously across the globe. Fetal bovine serum (FBS) is a media supplement used for cell culture and is derived from whole blood obtained from usual bovine fetuses collected from healthy cows at slaughterhouses. It is a byproduct of meat processing industries. The key component of FBS is bovine serum albumin. The factors that are playing a major role in the growth of fetal bovine serum market are the growing demand from end-users and is easy for regulatory approvals. However, ethical issues during blood collection method and the environmental conditions may restrain the overall market in the years to come. The market is segmented based on application, end user, and region. The market may be categorized based on applications like diagnostics, drug discovery, human and animal vaccine production, cell culture media, in vitro fertilization, and others that ...
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Albumin is one of the most extensively used proteins in biological research today. It acts as a powerful antioxidant in cell culture. It binds, sequesters and stabilizes a variety of molecular species which are often unstable. This acidic, soluble protein has both high-affinity and secondary binding sites, optimizing the roles that fatty acids, metals, disulfides, and other molecules play in cellular metabolism. Seralab BSA products are prepared by heat shock with the exception of GEM-700-108 which is produced by a modified method of the Cohn cold ethanol fractionation method. Heat shock produces the highest purity BSA product Cohn fractionation method remains the gold standard - this method preserves the biological activity of albumin. Ready-to-buy solutions offer the convenience of not having to solubilise BSA in - house. Available in 20g, 100g and 1kg quantities (lyophilised); or 50mL, 100mL and 1L volumes (solution). Sourced serum or plasma is filtered through a 0.2 μm filter before
Features. *Manufactured according to FDA cGMPs. *For 1%, 3% and 5% fresh BSA solution. *Ultra low protease, endotoxin and IgG. *Preweighed and highly soluble. **International Serum Industry Association (ISIA) certified. *Ideal for immunoassay ( EIA, RIA, western blocker, immunofluorescent staining, etc), antibody dilution, enzyme stabilizer; protein standard; serology; mammalian cell culture. This small package of bovine serum albumin (BSA) is accurately pre-measured and easy to make 1%, 3% or 5% fresh blocking buffer. Compared to shorter shelf life in a large bottled package, individually packed BSA bags has a longer storage time of at least 5 years.. Our bulk order of BSA is a high purity, lyophilized Fraction V powder. This product has been tested and can be used in western blot, IHC, ICC, protease-sensitive Immunoassays such as RIA, EIA and ICF, protein standard, diluent and enzyme stabilizer , hybridization and selected cell culture applications.. Fresh blocking buffer made of preweighed ...
Essentially Fatty Acid Free; Produced by heat-shock isolation; Purity of ≥ 98%; CAS Number: 9048-46-8. Store at 2˚ to 8˚C.. Find this and many more products.
Albumin solution can be used as a medium in density gradient centrifugation or as an addition to tissue culture growth medium. Stable at least 2 years at 5°C. Sterile.Proteins are used in routine laboratory procedures such as binding enzymes or coupling peptides to carrier proteins. These kits, mixture solutions, and collagen matrices fulfill a myriad of essential laboratory functions for developing relationships between proteins and other cellular components. The stimulating proteins offered have various amino acid arrangements and functions to fulfill any sample manipulation for testing purposes in any field.
* found in: Bovine Serum Albumin Standard, We offer two options for the BSA protein standards: Bovine Serum Albumin Standard BSA standard (2mg/ml)..
Geminis Cohn fraction BSA is made by a modification of the original cold-ethanol plasma fractionation process developed in 1939 by Professor Edwin Cohn. Its characteristics include a minimum 96% albumin, with a pH of 7.0 - 7.4. This product is suitable as a growth nutrient in tissue culture media, for increasing the viscosity and protein levels in certain antibiotic assay media, to bind inhibitors in growth media, and as a molecular-weight standard. Ships ambient. Store refrigerated at 2 to 8°C CAS Number 9048-46-8 ...
Cold alcohol isolation suitable for sensitive biochemical and diagnostic assays. DNase and RNase free, virtually free of globulins and other interfering contaminants.
Proliant Biologicals line of Ultra-High Monomer BSA solutions are focused on the IVD industrys goals for improved assay sensitivity and earlier analyte detection. Continuing to set the standard for BSA manufacturers around the globe, Proliant operates two dedicated manufacturing facilities (Boone, Iowa and Feilding, New Zealand). Utilizing Proliants proprietary Closed Loop process, all BSA manufactured meets and exceeds the stringent ISIA Certified traceability standards. The Ultra High Monomer BSA Solutions are specifically designed to assist researchers & manufacturers increase the performance of their BSA in critical reagents while reducing nonspecific binding & assay background interference.. ...
Whats the Big Idea? Understanding the Laboratory Experience in the AP* Chemistry Curriculum If you teach AP* Chemistry, youre already aware, or need to be, that changes to the course curriculum are here, which means youll probably have to change your classroom instruction. To help relieve your anxiety, heres an overview of the course revisions, some important dates to remember, and 2 ways Carolina can help to ensure your success during this transition. View » ...
Whats the Big Idea? Understanding the Laboratory Experience in the AP* Chemistry Curriculum If you teach AP* Chemistry, youre already aware, or need to be, that changes to the course curriculum are here, which means youll probably have to change your classroom instruction. To help relieve your anxiety, heres an overview of the course revisions, some important dates to remember, and 2 ways Carolina can help to ensure your success during this transition. View » ...
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We are pleased to welcome you to our new website. As a thank you for your continued customer loyalty, all newly registered accounts will receive a complimentary 5% discount.. ...
Abstract The use of bovine serum albumin (BSA) in routine biochemical assays such as restriction enzyme digestion and immunodetection is plagued largely by two common contaminants, DNase and immunoglobulins G (IgGs). Acetylation of BSA
The deletion mutation was introduced into the ABI 2.1 embryonic stem (ES) cell line (Soriano et al., 1991) and transmitted to the germline as described previously (Bullard et al., 1996). Chimeric mice were bred with C57BL/6J mice, and the mice used in these studies were maintained on a mixed 129/SvEv and C57BL/6J background. The mutation is being back-crossed on the C57BL/6J background for future work.. Southern, Northern, and Western analyses. Southern blot hybridization was performed according to standard methods (Sambrook et al., 1989) using a hybridization solution of 0.125m NaPO4, pH 7.0, 0.25 mNaCl, 1 mm EDTA, 10% polyethylene glycol (PEG-8000), 7% SDS, and 1% bovine serum albumin (BSA) at 65°C overnight followed by washing to a final stringency of 0.2× SSC/0.1% SDS at 65°C and autoradiography at −80°C.. Total RNA was isolated from brain after homogenization in Ultraspec II (Tel Test, Houston, TX). Total RNA was resolved on a 1.2% agarose gel in 10 mm NaPO4 buffer, pH 6.8, after ...
The Cotinine-4 - BSA product is an Antigen Conjugate based on a BSA (Bovine Serum Albumin) carrier, attached to proprietary Cotinine derivatives
Fetal Bovine Serum USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma; Synonyms: FCS,serum,FBS,sera; find Sigma-Aldrich-F2442 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
TY - JOUR. T1 - Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses. AU - Bai, Wei. AU - Wu, Zheqiong. AU - Mitra, Somenath. AU - Brown, Jared M.. N1 - Funding Information: This work was supported by grants from the National Institutes of Health (R01 ES019311, R01 ES023209, and R03 ES023036). Publisher Copyright: © 2016 Wei Bai et al.. PY - 2016. Y1 - 2016. N2 - Carboxylation of multiwalled carbon nanotubes (MWCNTs) has been used to improve solubility in aqueous systems and for further functionalization with biologically active moieties for biomedical uses. An important consideration is that oxidation debris is generated during the process of carboxylation, which can be removed by base washing. We hypothesized that surface modification as well as purification by debris removal may alter physicochemical properties of MWCNTs and their ability to bind proteins. We utilized pristine MWCNT, carboxylated MWCNTs ...
Looking for online definition of bovine serum albumin in the Medical Dictionary? bovine serum albumin explanation free. What is bovine serum albumin? Meaning of bovine serum albumin medical term. What does bovine serum albumin mean?
The specific interfacial area of bovine serum albumin (BSA) adsorbed on montmorillonite was deduced from the ratio between the quantity of cations exchanged on adsorption of BSA and the quantity of protein bound. A paramagnetic cation, Mn2+, was used, and its release from the clay surface was followed by measuring the line broadening effect of displaced Mn2+ on the 31P NMR signal from orthophosphate in the solution. From experiments conducted at different protein/clay ratios and different pH, it was deduced: (i) that no more than one monolayer was adsorbed; (ii) that the specific interfacial area was the same at low and at high surface coverage; (iii) that below the isoelectric point (i.e.p.) the specific interfacial area of BSA increased with decreasing pH, with a constant surface coverage of the clay surface; and (iv) that above the i.e.p. the surface coverage of the clay decreased with further increases in pH. It is assumed that electrostatic interactions between the protein and the clay surface play
Psoas muscle was quickly dissected adjacent to the femoral nerve, with the former tip region of the catheter located in the middle of the tissue block, and placed in a normoxic (i.e. , equilibrated with air), cooled (4°C) relaxing solution (solution 1: 10 mm EGTA, 3 mm Mg2+, 20 mm taurine, 0.5 mm dithiothreitol, 5 mm ATP, 15 mm phosphocreatine, 20 mm imidazole, and 0.1 m K+2-[N-morpholino]ethane sulfonic acid, pH 7.2). To assess mitochondrial respiration, we used a permeabilized muscle fiber technique.24Bundles of 2- to 5-mg fibers were excised from the surface of the psoas and then permeabilized in solution 1 with 50 μg/ml saponin added. The bundle was then washed twice for 10 min each time in solution 2 (10 mm EGTA, 3 mm Mg2+, 20 mm taurine, 0.5 mm dithiothreitol, 3 mm phosphate, 1 mg/ml fatty acid-free bovine serum albumin, 20 mm imidazole, and 0.1 m K+2-[N-morpholino]ethane sulfonic acid, pH 7.2) to remove saponin. All procedures were performed at 4°C with extensive stirring. The success ...
The binding characteristics of reduced hepatic membrane proteins for acetylated low-density lipoprotein (acetyl-LDL) and maleylated bovine serum albumin (Mal-BSA) have been examined. Two receptor activities were extracted from hepatic membranes in the presence of octyl beta-D-glucoside and beta-mercaptoethanol, and were separated by chromatography on Mal-BSA-Sepharose 4B. The receptors were revealed by ligand blotting. The active binding proteins had apparent molecular masses of 35 and 15 kDa in SDS/polyacrylamide gels. Equilibrium studies with protein-phosphatidylcholine complexes indicated that the reduced 35 kDa protein expresses two binding sites for Mal-BSA and one for acetyl-LDL, whereas the 15 kDa protein-phosphatidylcholine complex binds 131I-Mal-BSA and 131I-acetyl-LDL with a 4:1 stoichiometry. 131I-Mal-BSA binding was linear with both proteins, with a Kd of 4.8 nM at the 35 kDa protein and a Kd of 5.6 nM at the 15 kDa protein. The 35 kDa protein displayed saturable binding of ...
Bovine serum albumin (BSA) and fatty acid-free human serum albumin (HSAFAF) reduce the Km values for UGT2B7 substrates by sequestering inhibitory long-chain fatty acids released by incubations of human liver microsomes (HLM) and HEK293 cells expressing this enzyme. However, the scope of the albumin effect is unknown. In this investigation we characterized the effects of albumin on the kinetics of 4-methylumbelliferone (4MU) glucuronidation by UDP-glucuronosyltransferase (UGT) 1A1, 1A6, and 1A9, and propofol (PRO) glucuronidation by UGT1A9 and HLM. BSA and HSAFAF, but not human serum albumin, reduced the Km values for 4MU and PRO glucuronidation by UGT1A9. For example, HSAFAF (2%) reduced the Km values for 4MU and PRO glucuronidation from 13.4 to 2.9 and 41 to 7.2 μM, respectively. Similarly, HSAFAF (2%) reduced the Km for PRO glucuronidation by HLM from 127 to 10.6 μM. Arachidonic, linoleic, and oleic acids and a mixture of these decreased the rates of 4MU and PRO glucuronidation by UGT1A9. ...
TY - JOUR. T1 - A liposome reversibly coated with serum albumin. AU - Sato, Hikari. AU - Nakamura, Yuta. AU - Nakhaei, Elnaz. AU - Funamoto, Daiki. AU - Kim, Chan Woo. AU - Yamamoto, Tatsuhiro. AU - Kishimura, Akihiro. AU - Mori, Takeshi. AU - Katayama, Yoshiki. PY - 2014. Y1 - 2014. N2 - Herein, we reported a design of liposome affording a reversible coating of serum albumin on its surface. We synthesized a lipid modified with a serum albumin-specific endogenous ligand and prepared liposome, including this lipid. We successfully confirmed the coating of the liposome surface with serum albumin. The liposome presented here would be applicable to a drug carrier with enhanced blood circulation.. AB - Herein, we reported a design of liposome affording a reversible coating of serum albumin on its surface. We synthesized a lipid modified with a serum albumin-specific endogenous ligand and prepared liposome, including this lipid. We successfully confirmed the coating of the liposome surface with serum ...
Layer-by-layer structures (e.g. polyelectrolyte multilayers and protein-polyelectrolyte multilayers) play a very important role in surface modification processes. In the present study, electrokinetic measurements were applied for the investigation of poly(allylamine hydrochloride)/poly(sodium 4-styrenesulphonate) (PAH/PSS) multilayer formation, with PAH being a terminal layer, as a function of pH. Additionally, the effect of supporting electrolyte (KCl) concentration on multilayer formation was tested. Silica particles were used as the solid substrate. Furthermore, the adsorption of bovine serum albumin (BSA) on previously formed multilayer was examined as a function of pH and BSA concentration. It was confirmed that the electrokinetic measurements are suitable for monitoring the formation of various multilayers. In all investigated systems the process of multilayer formation was found to depend on conditions (ionic strength and pH) under which the multilayer was formed. Moreover, in the case of ...
Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection Zhipeng Chen,* Juan Chen,* Li Wu, Weidong Li, Jun Chen, Haibo Cheng, Jinhuo Pan, Baochang CaiDepartment of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China*These authors contributed equally to this workObjective: To evaluate the potential of hyaluronic acid (HA)-coated bovine serum albumin nanoparticles (BSANPs) as a novel chondrocyte-targeting drug-delivery nanomedicine.Methods: The HA-BSANPs were characterized by dynamic light scattering, transmission electron microscopy, differential scanning calorimetry, and X-ray diffraction. Fluorescence imaging was used to visualize the distribution of nanoparticles after intra-articular injection. The chondrocyte-targeting efficiency and cellular uptake mechanism of HA-BSANPs were investigated using endocytic inhibitors.Results: HA-BSANPs were successfully prepared with HA coating the
TY - JOUR. T1 - Adsorption and Conformation of Bovine Serum Albumin with Blue-Emitting Gold Nanoclusters at the Air/Water and Lipid/Water Interfaces. AU - Putra, Ridwan P.. AU - Ikumura, Yoshinori. AU - Horino, Hideyuki. AU - Hori, Akiko. AU - Rzeznicka, Izabela I.. PY - 2019/12/17. Y1 - 2019/12/17. N2 - Protein-encapsulated nanoclusters (NCs) are emerging as a versatile platform for in-vivo imaging and other biomedical applications due to their ultrasmall size and excitation in the near-infrared region. Encapsulation may however affect protein structure, size, charge, and its interaction with lipid membranes. In this study, bulk characterization methods along with surface-sensitive vibrational sum-frequency generation (VSFG) spectroscopy were employed to study the secondary structure of bovine serum albumin (BSA) with blue-emitting Au8NCs at the air/water and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DPPG) lipid/water interfaces. With this approach, the difference in the ...
Influence of amphiphiles on surface energy (γ), emulsifying activity index (EAI) and emulsion stability (ES) of fatty-acid free bovine serum albumin (FAF-BSA), bovine serum albumin (BSA), and β-lactoglobulin A (β-LGA) was studied. Contact angle measurements (θ) of sessile drops of protein-surfactant mixtures were determined using water and α-bromonaphthalene as solvents of known parameters to calculate γ. All surfactants except zwitterionic Z8 increased γ. The greatest increase was by anionic SDS. All surfactants significantly reduced EAI in spite of increased γ. ES was reduced by all surfactants except cationic TDTM which, conversely, enhanced ES by up to 186% for BSA and β-LGA.
Learn more about Bovine Serum Albumin (Fraction V) and get the best prices in the life science marketplace at AG Scientific, Inc. We have over 20 years of experience with biochemical supplies that were excited to share with you!
TY - JOUR. T1 - Application of Fluorescence Spectroscopy to Quantify Shear-Induced Protein Conformation Change. AU - Themistou, Efrosyni. AU - Singh, Indrajeet. AU - Shang, Chengwei. AU - Balu-Iyer, Sathy V.. AU - Alexandridis, Paschalis. AU - Neelamegham, Sriram. PY - 2009/11/4. Y1 - 2009/11/4. N2 - Rapid and robust methods are required to quantify the effect of hydrodynamic shear on protein conformation change. We evaluated such strategies in this work and found that the binding of the fluorescent probe 4,4-dianilino-1, 1-binaphthyl-5,5-disulfonic acid (bis-ANS) to hydrophobic pockets in the blood protein von Willebrand factor (VWF) is enhanced upon the application of fluid shear to the isolated protein. Significant structural changes were observed when the protein was sheared at shear rates ,= 6000/s for similar to 3.5 min. The binding of bis-ANS to multimeric VWF, but not dimeric VWF or control protein bovine serum albumin, was enhanced upon fluid shear application. Thus, ...
Externally supplied protein (bovine serum albumin, BSA) affects root development of Arabidopsis, increasing root biomass, root hair length, and root thickness. While these changes in root morphology may enhance access to soil microenvironments rich in organic matter, we show here that the presence of protein in the growth medium increases the plants resilience to the root pathogen Cylindrocladium sp.. ...
An amperometric biosensor for L-glutamic acid (Glu) was constructed by the adsorption and dip coating of L-glutamate oxidase (GluOx, 200 U ml-1 phosphate buffer, pH 7.4) onto 60-µm radius Teflon-coated Pt wire (1 mm exposed length). The enzyme was then trapped on the surface by electropolymerisation of o-phenylenediamine that also served to block electroactive interference. This procedure afforded electrodes with similar substrate sensitivity compared with the classical approach of immobilising enzyme from a solution of monomer, and represents an approximately 10000-fold increase in the yield of biosensors from a batch of enzyme. A number of strategies were examined to enhance the sensitivity and selectivity of the Pt/PPD/GluOx sensors operating at 0.7 V versus SCE. Pre-coating the Pt with lipid and incorporation of the protein bovine serum albumin into the polymer matrix were found to improve the performance of the electrode. The sensors had a fast response time, high sensitivity to Glu, with ...
Fourier transform infrared (FT-IR) spectra have been measured for defatted bovine serum albumin (BSA) in D,sub,2,/sub,O with a concentration of 2.0 wt % over a temperature range of 26-90 °C and the corresponding difference spectra have been calculated by subtracting the contribution of D,sub,2,/sub,O at the same temperature. Evolving factor analysis (EFA) by selecting two factors and three factors has been employed to analyze the temperature-dependent difference IR spectra in the 1700-1600 cm,sup,-1,/sup, spectral region of the defatted BSA in D,sub,2,/sub,O solution. Three-factor EFA has been employed to determine the distinction of the three protein species involved in the process of temperature elevation: native, transitional, and denatured protein. The temperature profiles obtained from three-factor EFA indicate that heat-induced conformational change in the secondary structures of defatted BSA in D,sub,2,/sub,O undergoes two two-state transitions, a drastic transition and a slight ...
Nanochemistry offers stimulating opportunities for a wide variety of applications in the biosciences. Understanding of the interaction of nanoparticles with biomolecules such as proteins is very important as it can help better design and fabricate nanocomposites for applications in diagnostics, drug delivery, and cell monitoring. In this work, the interaction of Bovine Serum Albumin (BSA) and two types of metal oxide nanoparticles (titanium and tin) have been studied using the intrinsic fluorescence of tryptophan residue from the proteins measured by steady state and time resolved fluorescence techniques. The nanoparticles which were fabricated using a novel synthetic process have average sizes of ~2 nm (SnO2) and ~6 nm (estimated for TiO2) and have very high solubilities in a variety of solvents. The Stern-Volmer plots indicate an effective quenching process by TiO2 nanoparticles whereas SnO2 nanoparticles have a lower quenching efficiency for BSA fluorescence. Static quenching is the major ...
Bovine Serum Albumin (BSA) Antigen Capture ELISA - Photometric, 5 x 96 Tests. |p|The advent of biological products produced using animal components has created a need to ensure their removal prior to use.
Rivaroxaban is a direct inhibitor of coagulation factor Xa and is used for venous thromboembolic disorders. The rivaroxaban interaction with BSA was studied to understand its PK and PD (pharmacokinetics and pharmacokinetics) properties. Multi-spectroscopic studies were used to study the interaction which included UV spectrophotometric, spectrofluorometric and three dimensional spectrofluorometric studies. Further elucidation of data was done by molecular simulation studies to evaluate the interaction behavior between BSA and rivaroxaban. Rivaroxaban quenched the basic fluorescence of BSA molecule by the process of static quenching since rivaroxaban and BSA form a complex that results in shift of the absorption spectra of BSA molecule. A decline in the values of binding constants was detected with the increase of temperatures (298-308 K) and the binding constants were in range from 1.32 × 105 to 4.3 × 103 L mol−1 indicating the instability of the BSA and rivaroxaban complex at higher temperatures.
Rivaroxaban is a direct inhibitor of coagulation factor Xa and is used for venous thromboembolic disorders. The rivaroxaban interaction with BSA was studied to understand its PK and PD (pharmacokinetics and pharmacokinetics) properties. Multi-spectroscopic studies were used to study the interaction which included UV spectrophotometric, spectrofluorometric and three dimensional spectrofluorometric studies. Further elucidation of data was done by molecular simulation studies to evaluate the interaction behavior between BSA and rivaroxaban. Rivaroxaban quenched the basic fluorescence of BSA molecule by the process of static quenching since rivaroxaban and BSA form a complex that results in shift of the absorption spectra of BSA molecule. A decline in the values of binding constants was detected with the increase of temperatures (298-308 K) and the binding constants were in range from 1.32 × 105 to 4.3 × 103 L mol−1 indicating the instability of the BSA and rivaroxaban complex at higher temperatures.
It is generally believed that intravenous application of cationic vectors is limited by the binding of abundant negatively charged serum components, which may cause rapid clearance of the therapeutic agent from the blood stream. However, previous studies show that systemic delivery of cationic gene vectors mediates specific and efficient transfection within the lung, mainly as a result of interaction of the vectors with serum proteins. Based on these findings, a novel and charge-density-controllable siRNA delivery system is developed to treat lung metastatic cancer by using cationic bovine serum albumin (CBSA) as the gene vector. By surface modification of BSA, CBSA with different isoelectric points (pI) is synthesized and the optimal cationization degree of CBSA is determined by considering the siRNA binding and delivery ability, as well as toxicity. The CBSA can form stable nanosized particles with siRNA and protect siRNA from degradation. CBSA also shows excellent abiliies to intracellularly ...
SPR has been demonstrated to be a powerful optical technique for bioaffinity studies at the solid/solution interface. This application note describes the kinetic studies of ferulic acid and bovine serum albumin (BSA) pre-immobilized onto an alkanethiol self-assembled monolayer (SAM)-modified gold sensor chips.
We determined whether primed and unprimed B cells in the spleen of (BALB/c × C57BL/Ka)F(1) mice contain subpopulations that express a predominant surface Ig isotype. Spleen cells were stained for surface isotypes and sorted on the fluorescence-activated cell sorter (FACS) in order to obtain B cells bearing predominantly IgM (μp cells), IgD (δp cells), or IgG (γp cells). Each population was assayed for its capacity to restore the adoptive primary and secondary anti-bovine serum albumin (BSA) antibody response in irradiated syngeneic recipients. In addition, the adoptive response restored by isotype-predominant cells was compared to that restored by isotype- positive cells (B cells bearing a given surface isotype alone or in combination with others). The experimental results show that μp cells restore the adoptive primary and secondary IgM and IgG responses to BSA, and γP cells restore only the primary and secondary IgG response. Δp Cells restored the adoptive secondary IgG response, but ...
This fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States. Sterility tests are performed on each lot of fetal bovine serum using current USP and EP methods. Specific tests for contaminants are listed below in the Specification Certificate.   This serum is tested for ability to support the growth and maintenance of embryonic stem cells in an undifferentiated state.  Undifferentiated embryonic stem cells are determined by colony morphology and expression of three pluripotent markers.  The results are compared to a control lot of serum. The serum is triple filtered through 0.1 μM filters and each lot of fetal bovine serum is tested for sterility. Origin This fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States. Test* Specification Sterility Testing (Current USP and EP) Bacteria and Fungi No growth Virus Testing (9 CFR 113.53) Fluorescent
Membranous and/or proliferative pneumonitis, similar in certain features to human interstitial pneumonitis, developed in rabbits making hyperactive antibody response to daily injections of bovine serum albumin (BSA) administered in multiple large doses sufficient to maintain the state of relative antigen-antibody equivalence. The pulmonary lesions were associated with deposition in alveolar capillary walls and interstitium of antigen, host globulin and complement, presumably in immune complexes. In some rabbits chronic interstitial pneumonitis, characterized by thickening of alveolar capillary walls, interstitial fibrosis and deposition of fibrinogen, was observed. The production of immune complex pneumonitis seems to depend on the degree of the antibody response because rabbits developing chronic serum sickness with low doses of BSA, rabbits with acute serum sickness as well as nonresponders showed no pulmonary alterations. This observation is comparable to that described by Dixon in his ...
* found in: Bovine Albumin, Fraction V, Protease-Free, Bovine Albumin, Cohn Modified, Lyophilized, Ovalbumin from Chicken Egg =80% Purity, Bovine Albumin,..
Calf bovine serum, iron fortified is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Blood is collected from donor animals by venipuncture between the ages of 16 and 22 weeks. Calf bovine serum is supplemented with iron to off-set the high concentrations of transferrin produced by formula-fed animals prior to collection. Sterility tests are performed on each lot of calf bovine serum using current USP and EP methods. Each lot is also checked for its ability to support the growth of cells. Specific testing for contaminants is listed below in the Specification Certificate. Origin This calf bovine serum is manufactured from calf bovine blood collected in USDA-inspected abattoirs located in the United States. Test* Specification Sterility Testing (Current USP and EP) Bacteria and Fungi No growth Virus Testing (9 CFR 113.53) Fluorescent antibody Bluetongue Bovine Adenovirus Bovine Parvovirus Bovine Respiratory Syncytial Virus Bovine Diarrhea
Concentrated solution of biotinylated Bovine Serum Albumin (BSA) conjugated to Tacrolimus (FK506). A carbon spacer arm of 19.0 Å links BSA to the Tacrolimus moiety. Each BSA molecule contains several Tracrolimus units. Dilutions of the provided conjugate can be made in 1X Phosphate Buffer Saline, pH 7.4. This conjugate
Cells were harvested and proliferation and secreted cytokines analysed as described Lenvatinib supplier previously. Proteins were immobilized on the beads, as per the manufacturers instructions. Briefly, 0·5 ml of the provided Dynabeads were washed twice with phosphate-buffered saline (PBS), resuspended in 200 µl of PBS per tube, and 20 µg of anti-CD3ε and/or the indicated µg amount of anti-BTLA test antibody (or antibodies) reagent was absorbed passively to the beads, mixed well and incubated at room temperature for 60 min. The tube was vortexed (bench top) every 3 min to ensure. mixing. Then 100 µl of a 0·5% bovine serum albumin (BSA) solution in PBS was added to each tube and the volume adjusted to 500 µl with PBS to block any unoccupied bead surface. The beads were incubated at 4°C for. 3 days with shaking and then washed three times with 0·1% BSA in PBS buffer. They were finally resuspended in 500 µl of 0·1% BSA in PBS to yield a final bead concentration of 4 × 108/ml and the ...
TY - JOUR. T1 - Antibody synthesis by transferred lymphoid cells. T2 - The influence of the host genetic environment on the duration of the immune response. AU - Wakefield, June D.. AU - Rose, Noel R.. PY - 1968. Y1 - 1968. N2 - Spleen cells from 129/JaxHe and C3H/He mice, actively immunized with bovine serum albumin, were transferred to 129/ JaxHe, C3H/He, and F1 hybrids of these strains. Grafts of spleen cells from the two strains showed similar patterns of behavior, in that the antibody to bovine serum albumin was detectable for at least 60 days in syngenie recipients when male spleen cells were transferred to male recipients. Females accepted male cells transiently; antibody rarely was present at the 4th week. Parental spleen cells produced antibody for limited periods of 1 month in F1 mice. The two mouse strains differed in their responsiveness to certain grafts of skin. Female 129 mice rejected male 129 skin grafts, whereas C3H females appeared to accept grafts of C3H male skin. Such ...
The animal multiples of human exposure calculations are based on body surface area (BSA) comparisons of oral doses administered to animals and an EXTINA Foam maximum recommended human dose (MRHD) of 8 grams (equivalent to 2.67 mg ketoconazole/kg/day for a 60 kg individual or 98.8 mg ketoconazole/m2/day).. Embryofetal development studies have been conducted in mice, rats and rabbits with orally administered ketoconazole. When orally administered to mice on gestational days 6 through 18 (covering the period of organogenesis), ketoconazole was embryotoxic (25 mg/kg and higher; 0.8 times the MRHD based on BSA comparisons) with a high incidence of resorptions, increased number of stillbirths and delayed parturition. Delays in maturation were also observed. There was no evidence of maternal toxicity or malformations at up to 50 mg/kg (1.5 times the MRHD based on BSA comparisons). No treatment related developmental effects were observed at 10 mg/kg (0.3 times the MRHD based on BSA comparisons).. In the ...
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dyes functional group. See the product information sheets for specific reactive dyes more information.. Coelenterazines and D-luciferin. Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also ...
Correspondence re: D. A. Beck, R. D. Rossen, D. B. Dubois, and C. O. Felice. Synthesis of Antigens, Cross-Reactive with Bovine Serum Albumin, by Cultured Neuroblastoma Cells. Cancer Res. 43: 858-863, 1983; D. A. Beck, R. D. Rossen, A. Cangir, and D. B. Dubois. Correlation of Immune Complexes in Disseminated Neuroblastoma with Serum Antibody to Bovine Serum Albumin. Cancer Res., 43: 879-885, 1983 ...
The objective of this study is to investigate the impact of Ultra-High Pressure (UHP) combined with moderate heat treatment on the allergenic properti..
Fetal Bovine Serum (FBS) Central America, USDA approved (100 ml500 ml) by Biowest, The Serum Specialist. Purchase online or contact us !
Human Serum, AB Serum, Fetal Bovine Serum, Antibiotic Solutions, Albumin, Plasma, Serum, Blood, Reagents, Aqueous Solutions, Media, Clinical Lab Products, Contract Manufacturer
Get accurate and quick report of Serum Albumin Test in ahmednagar at your nearest Metropolis lab or your home at affordable cost. The serum albumin test looks at the levels of albumin in a persons blood.
Get accurate and quick report of Serum Albumin Test in Navsari at your nearest Metropolis lab or your home at affordable cost. The serum albumin test looks at the levels of albumin in a persons blood.
Human Serum, AB Serum, Fetal Bovine Serum, Antibiotic Solutions, Albumin, Plasma, Serum, Blood, Reagents, Aqueous Solutions, Media, Clinical Lab Products, Contract Manufacturer