Background Pneumococcal serotype identification is essential to monitor pneumococcal vaccine effectiveness and serotype replacement. Sequetyping PCR failed in 7/40 (18%) Jaceosidin manufacture isolates. For the rest of the isolates, sequetyping designated the right serotype/-group to 29/33 (88%) control isolates. From the 132/135 (98%) nasopharyngeal pneumococcal isolates that may be typed, 69/132 (52%) and 112/132 (85%) had been assigned the right serotype/-group by rmPCR and sequetyping respectively. The serotypes of 63/132 (48%) isolates werent contained in the rmPCR -panel. All except three isolates (serotype 25A and 38) had been theoretically amplified and differentiated in to the right serotype/-group with some strains providing ambigous outcomes (serotype 13/20, 17F/33C, and 11A/D/1818F). From the pneumococcal serotypes recognized with this scholarly research, 69/91 (76%) werent contained Jaceosidin manufacture in the current PCV13. Probably the most determined serotypes had been 11A ...
The Salmonella Serotyping Assay (SSA) is a multiplex, nucleic acid-based assay for use in identifying the serotype of a Salmonella isolate.
The purpose of this study was to develop a simple and non-labour-intensive molecular method to identify the phase 1 H antigens of Salmonella. The variable region of the flagellin gene, fliC, from 96 Salmonella strains representing 51 different phase 1 H antigens was sequenced in one direction. Unique sequences were found for 45 of the 51 different antigens. We were not able to separate either H:z42 from H:d; H:g, q from H:g, m, q; H:l, w from H:Rl, z40 or H:l, (v),z13 from H:l,z,13. Several phase 2 H antigens were found to be encoded by fliC. Polymorphism, at the subspecies level, was observed in fliC of H:b, H:d, H:z10, H:z and especially H:k. By this method we were also able to confirm that one monophasic strain possesses a new antigen, H:z91. This study shows that sequence-based typing of the phase 1 H antigen of Salmonella is a good alternative to serotyping when strains are non-typable by serological methods ...
Video created by Technical University of Denmark (DTU) for the course Whole genome sequencing of bacterial genomes - tools and applications. Whole genome sequencing tools- demonstration of analysis tools for Serotyping of Salmonella and ...
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2017 The Authors. The competitive pressure from non-vaccine serotypes may have helped pneumococcal conjugate vaccines (PCVs) to limit vaccine-Type (VT) serotype prevalence. We aimed to investigate if, consequently, the indirect protection of vaccines targeting most pneumococcal serotypes could fall short of the profound effects of current formulations.We compared three previously described pneumococcal models harmonized to simulate 20 serotypes with a combined pre-vaccination prevalence in children younger than 5-years-old of 40%. We simulated vaccines of increasing valency by adding serotypes in order of their competitiveness and explored their ability to reduce VT carriage by 95% within 10 years after introduction. All models predicted that additional valency will reduce indirect vaccine effects and hence the overall vaccine impact on carriage both in children and adults. Consequently, the minimal effective coverage (efficacy against carriage vaccine coverage) needed to eliminate VT carriage ...
We present here a parsimonious mechanistic model, developed as a generalization of existing deterministic models of pneumococcal transmission, which is capable of reproducing many of the distinct features of S. pneumoniae. We show that the means of non-specific and specific immunity are capable of governing the patterns of between-host competition and coexistence, and that stochastic effects in low prevalent serotypes may result in apparent epidemics, if these serotypes also have a high propensity to cause invasive disease (as has been observed in several countries with serotype 1, for instance). We further show that high carriage prevalence observed in developing country settings [25] and in native populations [36] might arise from a less effective immune response (owing to malnutrition, genetic differences or other factors) rather than differences in the number of contacts alone. Moreover, we use the model to assess the impact of vaccination, and contrast a vaccine targeted at the most ...
Adeno-associated virus (AAV) vectors of various serotypes are considered to have high potential for gene therapy applications. Currently, manufacturing
Ready-to-use AAV available from Addgenes viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1, AAV2, AAV5, AAV8 and AAV9
Serotype distribution and PCV immunization coverage among the S. pneumoniae isolates. a. Serotypes and immunization coverage rates of PCVs among all age groups.
CDC DENV-1-4 : real-time RT-PCR assay for detection and serotype identification of dengue virus : instructions for use package insert [Spanish] : El ensayo de RT-PCR en tiempo real para DENV-1-4 de los Centros para el Control y la Prevención de Enf ...
Science & Technology, Life Sciences & Biomedicine, Veterinary Sciences, VETERINARY SCIENCES, HIGH-PATHOGENICITY ISLAND, IN-VIVO EXPRESSION, FIBRONECTIN-BINDING, SERUM RESISTANCE, P-FIMBRIAE, FAMILY ENTEROBACTERIACEAE, SALMONELLA-ENTERITIDIS, COMPLEMENT RESISTANCE, AGGREGATIVE FIMBRIAE, CURLI PRODUCTION ...
ViroTag® ADVX (for manual sampling) utilizes a fluorescently-labeled, high-affinity antibody which binds to a unique epitope specifically expressed on adenovirus. The product has been shown to quantify multiple serotypes (2-6) and is considered pan-reactive. With the Virus Counter 3100, use this rapid, no-wash labeling procedure and take adenovirus quantification to new levels of accuracy, speed and simplicity!. Product specifications: The ViroTag ADVX kit (catalog number 92097) contains all reagents and consumables necessary to analyze 200 samples using the Virus Counter 3100 instrument for manual sampling, including:. ...
abms RNAi Expression System is now available in Adeno-Associated Virus format, combining the efficient expression of any siRNA with the advantage of non-integrating AAV for a new, powerful gene knockdown tool for gene therapy. The system is based on a unique convergent promoter design for greater efficiency of target gene knockdown without the need for a hair-pin loop structure commonly utilized in single promoter vectors, and includes a GFP reporter for monitoring transduction. abm offers a comprehensive library of human, mouse, and rat siRNAs in vector or pre-packaged AAV formats, available in multiple serotypes for tissue-specific knockdown.
Hi, I am trying to locate a commercial source for rabbit polyclonal antibodies to HIV P24 and gp120. I hope to use these in Western blots of clinical iso- lates and would hope that they would have cross-reactivity with multiple serotypes. Thanks, --Tom ...
Isolation, serotyping, antimicrobial minimum inhibitory concentration and phatogeneticity determination of erysipelothrixrhusiopathiae from tonsils of apparently healthy slaughter pigs.
Some of the worlds top scientists are gathering at Penn State next week for an invitation-only workshop to discuss how to reach a consensus on techniques to identify new serotypes of E. coli. Penn States 50-year-old E. coli Reference Center is hosting the international meeting. "We hope the workshop here can lead to a consensus," said… Continue Reading. ...
Some of the worlds top scientists are gathering at Penn State next week for an invitation-only workshop to discuss how to reach a consensus on techniques to identify new serotypes of E. coli. Penn States 50-year-old E. coli Reference Center is hosting the international meeting. "We hope the workshop here can lead to a consensus," said… Continue Reading. ...
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two. Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa. The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to. XR1.3-E531K ...
AAV serotypes 1 and 6 share 99% homology in that only a 6 amino acid difference separates the two. Researchers were able to individually change these amino acids to represent the residue expressed by the other serotype and vice versa. The amino acids and their changes are listed below for this plasmid and include the parental residue, position of the residue, and residue changed to. XR1.4-F584L ...
The ICS program continued to expand in 2007. Monitoring rates of disease and levels of antimicrobial resistance in S. pneumoniae, H. influenzae, N. meningitidis, GAS and GBS via use of the ICS system is important in providing data on groups at risk for disease, measurement of effectiveness of prevention measures, and emerging challenges in serotype distribution and antimicrobial resistance. Efforts to expand ICS to include all circumpolar nations will continue ...
Definition of quellung reaction in the Definitions.net dictionary. Meaning of quellung reaction. What does quellung reaction mean? Information and translations of quellung reaction in the most comprehensive dictionary definitions resource on the web.
Streptococcus suis (S. suis) is a major swine pathogen and an emerging zoonotic agent. Serotypes 1, 2, 3, 7, 9, 14 and 1/2 are the most prevalent serotypes of this pathogen. However, almost all studies were carried out on serotype 2 strains. Therefore, characterization of genomic features of other serotypes will be required to better understand their virulence potential and phylogenetic relationships among different serotypes. Four Chinese S. suis strains belonging to serotypes 1, 7, 9 and 1/2 were sequenced using a rapid, high-throughput approach. Based on the 13 corresponding serotype strains, including 9 previously completed genomes of this bacterium, a full comparative genomic analysis was performed. The results provide evidence that (i) the pan-genome of this species is open and the size increases with addition of new sequenced genomes, (ii) strains of serotypes 1, 3, 7 and 9 are phylogenetically distinct from serotype 2 strains, but all serotype 2 strains, plus the serotype 1/2 and 14 strains, are
A number of investigators in a variety of countries have compared these two serotyping protocols in terms of the strengths and advantages of each and what they have achieved since their introduction; however, it is difficult to compare O antigen typing results to those of HL antigen typing given that each scheme is used to detect different antigens on the bacterium. In 1985, Patton et al. (21) performed a comparative study using the Penner and Lior methods for serotyping Campylobacter. Their findings indicated that 96.1% of isolates were typeable by the Penner method and that 92.1% were typeable by the Lior method. In 1993, a second study by the same group determined that, of a representative sample of 298 Campylobacter isolates from across the United States, a total of 24 O antisera were needed to serotype 84.6% of the strains by the HS scheme (20). Among the most common serotypes were O:1 (or O:1,8), O:13,16,43,50, O:8 (or O:8,17), O:4, O:5−,5+, O:2, O:3, O:6,7,25,29, O:19, and O:15,38. One ...
TY - JOUR. T1 - Diagnostic accuracy of a serotype-specific antigen test in community-acquired pneumonia. AU - Huijts, S.M.. AU - Pride, M.W.. AU - Vos, J.M.. AU - Jansen, K.U.. AU - Webber, C.. AU - Gruber, W.. AU - Boersma, W.G.. AU - Snijders, D.. AU - Kluijtmans, J.A.J.W.. AU - van der Lee, I.. AU - Kuipers, B.A.. AU - van den Ende, A.. AU - Bonten, M.J.M.. PY - 2013. Y1 - 2013. U2 - 10.1183/09031936.00137412. DO - 10.1183/09031936.00137412. M3 - Article. C2 - 23397295. VL - 42. SP - 1283. EP - 1290. JO - European Respiratory Journal. JF - European Respiratory Journal. SN - 0903-1936. IS - 5. ER - ...
From the CDCs Pink Book:. Streptococcus pneumoniae bacteria are lancet-shaped, gram-positive, facultative anaerobic organisms. They are typically observed in pairs (diplococci) but may also occur singularly or in short chains. Some pneumococci are encapsulated, their surfaces composed of complex polysaccharides. Encapsulated organisms are pathogenic for humans and experimental animals, whereas organisms without capsular polysaccharides are not. Most S. pneumoniae serotypes have been shown to cause serious disease, but only a few serotypes produce the majority of pneumococcal infections. The 10 most common serotypes are estimated to account for about 62% of invasive disease worldwide. The ranking and serotype prevalence differ by patient age group and geographic area. In the United States, the seven most common serotypes isolated from blood or cerebrospinal fluid (CSF) of children younger than 6 years of age account for 80% of infections. These seven serotypes account for only about 50% of ...
TY - JOUR. T1 - A mathematical model for the spread of Strepotococcus pneumoniae with transmission dependent on serotype. AU - Greenhalgh, David. AU - Lamb, Karen Elaine. AU - Robertson, Christopher. PY - 2012. Y1 - 2012. N2 - We examine a mathematical model for the transmission of Streptococcus Pneumoniae amongst young children when the carriage transmission coefficient depends on the serotype. Carriage means pneumococcal colonization. There are two sequence types (STs) spreading in a population each of which can be expressed as one of two serotypes. We derive the differential equation model for the carriage spread and perform an equilibrium and global stability analysis on it. A key parameter is the effective reproduction number R e. For R e ≤ 1, there is only the carriage-free equilibrium (CFE) and the carriage will die out whatever be the starting values. For R e , 1, unless the effective reproduction numbers of the two STs are equal, in addition to the CFE there are two carriage ...
Antisera against hexons of serotypes 2, 4, 5, and 6 (subgroup III), and 15 (subgroup II) were absorbed with purified hexons of various serotypes representing the different subgroups of human adenoviruses. Group, subgroup, and type specificities of hexons could be distinguished. The subgroup specificity of type 4 hexons resembled that of hexons of subgroup I members (types 3, 11, and 16). Antihexon sera gave a type-specific inhibition of virion-associated hemagglutinin. The inhibiting activity of different sera was found to be inversely related to the length of fibers of the serotype concerned. Virions of serotypes carrying fibers shorter than about 20 nm (types 3, 4, 9, 11, and 15) were readily inhibited, whereas those of serotypes with longer fibers (types 2 and 6) were inhibited only by relatively large amounts of antibody measured in terms of homotypic complement fixation activity. The reciprocal cross-neutralization between serotypes 4 and 16 was studied separately. Hexons of both serotypes ...
Recent nasopharyngeal colonization with a new serotype almost always precedes infection.4,5 Colonization rates are highest in infants and preschool children, where they may be as high as 35%.4 Children may carry different serotypes at different times but are almost always colonized by those limited serotypes responsible for infections in this age group.2 Carriage rates are lower in older children (40-50%) and in adults (5-30%).6,7 PCV7 includes the 7 most common serotypes causing colonization and infection in children (4, 6B, 9V, 14, 18C, 19F, and 23F). These serotypes and the cross-reactive serotypes (6A, 9A, 9L, 18B, 18F) caused 86% of cases of bacteremia, 83% of cases of meningitis, and 65% of cases of acute otitis media in children younger than 6 years.3 These serotypes also accounted for more than 80% of penicillin-resistant colonizing serotypes.8 ...
Looking for quellung? Find out information about quellung. Swelling of the capsule of a bacterial cell, caused by contact with serum containing antibodies capable of reacting with polysaccharide material in the... Explanation of quellung
Adeno-associated viruses (AAVs) are a class of single stranded DNA viruses that are not able to replicate without a helper virus. This makes them a perfect tool for the iGEM community, as no special safety requirements have to be fulfilled to work with a non-replication virus, because it is non-pathogenic. Because of their wide range of tropism they are used for transgene delivery in a variety of gene therapeutic approaches. In the class of AAVs, there are several serotypes that have been isolated from humans or non-human primates, the first and most well-known of them being AAV2. AAV serotypes are defined as naturally evolved variants of AAV that do not react to the same antibodies. All serotypes show different tissue specificites when injected into mouse or humans, and this tissue tropism is thought to be mainly due to interactions between the virus capsid and receptors on the cell surface. Most AAVs exhibit a rather broad tropism, AAV2 and AAV9 for example have been shown to transduce liver, ...
Despite the fact that more than 180 serotypes were characterised in this laboratory, the majority of incidents were due to only a few serotypes: 13 serotypes were responsible for 67.7 % of the total incidents (Table 1). Serovar Typhimurium was by far the most common, being recorded in 917 incidents (representing 26.8 % of the total), the majority of which occurred in poultry (598 incidents) in which it accounted for 65 % of the incidents. It was also the most common serotype reported in cattle (187 incidents), pigs (90 incidents) and sheep (42 incidents). As expected, the host-adapted serotypes such as S. Dublin, S. Choleraesuis and S. Enteritidis were almost exclusively isolated from cattle, pigs and poultry respectively. The low numbers of S. Gallinarum isolated from chickens was unusual but not unexpected. Biotyping methods identified these bacteria as S. Gallinarum and not S. Pullorum. Serovar Gallinarum is a common cause of septicaemia in layers and is frequently isolated by laboratories ...
About half of all WAIHA cases will have an autoantibody that reacts with all cells tested, including donor cells. The presence of an IgG autoantibody can be confirmed by elution. Elution is the process by which RBC-bound antibody is removed from the red cells and recovered, being sure that antibody reactivity is maintained so that antibody specificity can be determined. The eluate is usually reactive with all cells tested. Most IgG autoantibodies have an Rh-like specificity, such as anti-e. In order to identify the specific antibody, the laboratory would need to have a supply of rare cells such as Rhnull and D-- cells. Other specificities include those to high incidence antigens or a null phenotype. Examples include autoanti-U, autoanti-Wrb, autoanti-Ena, autoanti-Kpb, and autoanti-Vel ...
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Two hundred and thirty-two strains of Escherichia coli belonging to infantile enteropathogenic serotypes isolated in the United Kingdom during 1980 and 1981 were tested for resistance to 10 antimicrobial drugs. Resistance to one or more drugs was found in 134 (57.8%) of the strains, with resistance to sulphonamides, streptomycin, tetracycline, and ampicillin occurring most commonly. Resistance was transferable in 65 out of 104 resistant strains. These findings are a cause for concern because they indicate that the choice of treatment for severe illness is limited and suggest that a large pool of drug-resistant organisms exists in the community. ...
Book Hotel Marco Polo, Muenster on TripAdvisor: See 6 traveller reviews, candid photos, and great deals for Hotel Marco Polo, ranked #34 of 48 hotels in Muenster and rated 4 of 5 at TripAdvisor.
A biovar is a variant prokaryotic strain that differs physiologically or biochemically from other strains in a particular species.[1][2] Morphovars (or morphotypes) are those strains that differ morphologically. Serovars (or serotypes) are those strains that have antigenic properties that differ from other strains. ...
ONCOS-102 (previously known as CGTG-102) is a genetically modified replication competent oncolytic human adenovirus based on serotype 5. It is armed with GMCSF transgene and has a 24 bp deletion restraining the replication exclusively in tumors. The viral capsid has been modified for effective transduction of tumor cells. ...
Identification of the specific O serogroup(s) responsible for the positive STEC result and verifies that all targets relevant to the definition of a Top 6 STEC (eae, stx and O group) are present within a single ...
Abstract(#br) Background(#br) Streptococcus pneumoniae serotype 5 is among the most common serotypes causing invasive pneumococcal disease (IPD) in The Gambia. We anticipate that introduction of the 13-valent pneumococcal conjugate vaccine (PCV-13) into routine vaccination in The Gambia will reduce serotype 5 IPD. However, the emergence of new clones that have altered their genetic repertoire through capsular switching or genetic recombination after vaccination with PCV-13 poses a threat to this public health effort. In order to monitor for potential genetic changes post-PCV-13 vaccination, we established the baseline population structure, epidemiology, and antibiotic resistance patterns of serotype 5 before the introduction of PCV-13.(#br) Methods(#br)Fifty-five invasive S. pneumoniae... serotype 5 isolates were recovered from January 2009 to August 2011 in a population-based study in the Upper River Region of The Gambia. Serotyping was done by latex agglutination and confirmed by ...
Background: To date, more than 90 Streptococcus pneumoniae (S. pneumoniae) capsular serotypes are known. The prevalence of these serotypes varies according to the geographical area and the regional vaccination program. Due to the lack of regular vaccination programs for S. pneumoniae in developing countries, serotyping of the prevalent isolates is useful in selecting the correct vaccine. The present study aimed to evaluate common serotypes of pneumococcal meningitis in Bojnurd, Iran.Methods: All cerebrospinal fluid (CFS) samples suspected for bacterial meningitis were analyzed. The samples were collected during 2014-2018 in the Laboratory of Imam Reza Hospital (Bojnurd, Iran). Due to the high rate of false-negative cultures, polymerase chain reaction (PCR) was used for the detection of lytA and psaA genes of S. pneumoniae. In addition, the modified Marimons PCR method was used for serotyping the bacteria. The data were analyzed using Pearsons Chi-square test. Results: Out of the 901 CSF samples, 106
The Quellung reaction, also called the Neufeld reaction, is a biochemical reaction in which antibodies bind to the bacterial capsule of Streptococcus pneumoniae, Klebsiella pneumoniae, Neisseria meningitidis, Bacillus anthracis, Haemophilus influenzae, Escherichia coli, and Salmonella. The antibody reaction allows these species to be visualized under a microscope. If the reaction is positive, the capsule becomes opaque and appears to enlarge. Quellung is the German word for "swelling" and describes the microscopic appearance of pneumococcal or other bacterial capsules after their polysaccharide antigen has combined with a specific antibody. The antibody usually comes from a bit of serum taken from an immunized laboratory animal. As a result of this combination, and precipitation of the large, complex molecule formed, the capsule appears to swell, because of increased surface tension, and its outlines become clearly demarcated. The pneumococcal Quellung reaction was first described in 1902 by the ...
In humans, Streptococcus agalactiae or group B streptococcus (GBS) is a frequent coloniser of the rectovaginal tract, a major cause of neonatal infectious disease and an emerging cause of disease in non-pregnant adults. In addition, Streptococcus agalactiae causes invasive disease in fish, compromising food security and posing a zoonotic hazard. We studied the molecular epidemiology of S. agalactiae in fish and other aquatic species to assess potential for pathogen transmission between aquatic species and humans. Isolates from fish (n = 26), seals (n = 6), a dolphin and a frog were characterized by pulsed-field gel electrophoresis, multilocus sequence typing and standardized 3-set genotyping, i.e. molecular serotyping and profiling of surface protein genes and mobile genetic elements. Four subpopulations of S. agalactiae were identified among aquatic isolates. Sequence type (ST) 283 serotype III-4 and its novel single locus variant ST491 were detected in fish from Southeast Asia and shared a 3-set
Streptococcus agalactiae (GBS) is a common pathogen to infect newborn, woman, the elderly, and immuno-compromised human and fish. 37 fish isolates and 554 human isolates of the GBS in 2007-2012 were investigated in serotypes, antibiotic susceptibility, genetic difference and pathogenicity to tilapia. PCR serotyping determined serotype Ia for all fish GBS isolates and only in 3.2 % (3-4.2 %) human isolates. For fish isolates, all consisted a plasmid less than 6 kb and belonged to ST7 type, which includes mainly pulsotypes I and Ia, with a difference in a deletion at the largest DNA fragment. These fish isolates were susceptible to all antimicrobials tested in 2007 and increased in non-susceptibility to penicillin, and resistance to clindamycin and ceftriaxone in 2011. Differing in pulsotype and lacking plasmid from fish isolates, human serotype Ia isolates were separated into eight pulsotypes II-IX. Main clone ST23 included pulsotypes II and IIa (50 %) and ST483 consisted of
Infectious bronchitis virus (IBV) causes an acute, highly contagious respiratory disease in chickens, which results in significant economic losses in commercial flocks. One of the major hurdles associated with the control of the disease is the continued emergence of new serotypes/variants due to mutation or recombination. This also makes the diagnosis of disease using conventional serological methods difficult. Therefore, there is a need for alternative vaccines and serotyping methods. ^ A multiplex PCR for Massachusetts and Arkansas serotypes of IBV was developed. Two serotype specific PCR products, 1026 bp for Massachusetts and 896 bp for Arkansas were amplified; the detection limits for both were 5 pg of viral RNA. By testing different isolates/serotypes of IBV and other avian bacterial and viral pathogens, the high specificity of the multiplex PCR was demonstrated. In addition, its ability to detect Massachusetts and Arkansas as co-infections was revealed. ^ To explore the potential use of
Despite the concern of replacement disease, notably by serotype 19A after 7-valent conjugate vaccine (PCV7) use, serotype 19A was increasingly recognized in Korean children before the introduction of PCV7. To understand the dynamics of serogroup 19 prevalence from 1991-2006, we serotyped 538 pediatric pneumococcal isolates. Serogroup 19 isolates (n = 126) were characterized by antimicrobial drug susceptibility, presence of mefA/ermB, and multilocus sequence typing. Overall, the proportion of serotype 19A isolates increased but serotype 19F decreased. Among children <5 years of age, the proportion of serotype 19A isolates in invasive pneumococcal disease increased from 0% in 1991-1994 to 8%-10% in 1995-2000, reached 26% in 2001-2003, and remained at 20% in 2004-2006 when vaccine coverage did not exceed 25% (p = 0.005 for trend). This study demonstrates that the expansion of multidrug-resistant ST320 was responsible for the increase in serotype 19A before PCV7 use.
TY - JOUR. T1 - Application of WGS data for O-specific antigen analysis and in silico serotyping of Pseudomonas aeruginosa isolates. AU - Thrane, Sandra Wingaard. AU - Taylor, Véronique L.. AU - Lund, Ole. AU - Lam, Joseph S.. AU - Jelsbak, Lars. PY - 2016. Y1 - 2016. N2 - Accurate typing methods are required for efficient infection control. The emergence of whole genome sequencing (WGS) technologies has enabled the development of genomics-based methods applicable for routine typing and surveillance of bacterial pathogens. In this study, we developed the Pseudomonas aeruginosa serotyper (PAst) program, which enabled in silico serotyping of P. aeruginosa isolates using WGS data. PAst has been made publically available as a web-service, and aptly facilitate high-throughput serotyping analysis. The program overcomes critical issues such as the loss of in vitro typeability often associated with P. aeruginosa isolates from chronic infections, and quickly determines the serogroup of an isolate based ...
Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide Academic Article ...
Author Summary Streptococcus pneumoniae, or pneumococcus, is an important pathogen worldwide and causes a wide range of diseases, mostly in young children and the elderly. There are 91 serotypes of pneumococcus, each of which produces a unique polysaccharide, called the capsule, that attaches to the bacterial surface and prevents it from being cleared by the host. The serotypes differ greatly in their prevalence in the human population. There is currently a vaccine, effective in infancy, which targets seven clinically important serotypes, but several types not covered by the vaccine are beginning to increase in carriage frequency. As a result, it is critical to understand why some serotypes are frequently carried in the human population while others are not. In this study, we find that the high-prevalence serotypes tend to be more heavily encapsulated and more resistant to killing by neutrophils. Significantly, we find that the biochemical properties of the different polysaccharides can be used to
The relative invasive disease potential of Streptococcus pneumoniae among children after PCV introduction: A systematic review and meta-analysis ...