We report genetic maps for diploid (D) and tetraploid (AtDt) Gossypium genomes composed of sequence-tagged sites (STS) that foster structural, functional, and evolutionary genomic studies. The maps include, respectively, 2584 loci at 1.72-cM (∼600 kb) intervals based on 2007 probes (AtDt) and 763 loci at 1.96-cM (∼500 kb) intervals detected by 662 probes (D). Both diploid and tetraploid cottons exhibit negative crossover interference; i.e., double recombinants are unexpectedly abundant. We found no major structural changes between Dt and D chromosomes, but confirmed two reciprocal translocations between At chromosomes and several inversions. Concentrations of probes in corresponding regions of the various genomes may represent centromeres, while genome-specific concentrations may represent heterochromatin. Locus duplication patterns reveal all 13 expected homeologous chromosome sets and lend new support to the possibility that a more ancient polyploidization event may have predated the A-D ...

This track shows locations of Sequence Tagged Site (STS) markers along the draft assembly. These markers have been mapped using either genetic mapping (Genethon, Marshfield, and deCODE maps), radiation hybridization mapping (Stanford, Whitehead RH, and GeneMap99 maps) or YAC mapping (the Whitehead YAC map) techniques. Since August 2001, this track no longer displays fluorescent in situ hybridization (FISH) clones, which are now displayed in a separate track.. Genetic map markers are shown in blue; radiation hybrid map markers are shown in black. When a marker maps to multiple positions in the genome, it is shown in a lighter color.. ...

A continuous array of overlapping clones covering the entire human chromosome 21q was constructed from human yeast artificial chromosome libraries using sequence-tagged sites as landmarks specifically detected by polymerase chain reaction. The yeast artificial chromosome contiguous unit starts with pericentromeric and ends with subtelomeric loci of 21q. The resulting order of sequence-tagged sites is consistent with other physical and genetic mapping data. This set of overlapping clones will promote our knowledge of the structure of this chromosome and the function of its genes.

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These topics assume that Analysis Services is installed on a member server in a domain, instead of on a domain controller or on a stand-alone computer. Installing Analysis Services on a domain controller is not recommended for performance and security reasons. For more information about how to help secure the Windows operating system, see the Microsoft Security Web site. ...

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For each backup file, the user who runs the restore command must have permission to read from the backup location specified for each file. To restore an Analysis Services database that is not installed on the server, the user must also be a member of the server role for that Analysis Services instance. To overwrite an Analysis Services database, the user must have one of the following roles: a member of the server role for the Analysis Services instance, or a member of a database role with Full Control (Administrator) permissions on the database to be restored. ...

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Binding elements define out-of-line bindings, other than data sources and data source views, for Analysis Services objects to be processed by a Batch or Process command. For more information about processing objects, see Processing Objects (XMLA).. For more information about out-of-line bindings, see Data Sources and Bindings (Analysis Services - Multidimensional Data). ...

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We have generated 66 sequence-tagged-site (STS) markers from cDNA clones of Cryptomeria japonica, and 60% of them have already been mapped into C. japonica linkage groups. All of the STS markers show

ABSTRACT = { We examined the amount and nature of variation revealed by cDNA-based sequence-tagged-site (STS) markers in Norway spruce (Picea abies (L.) Karst.) using 39 pairs of heterologous primers that were based upon arbitrary genes in black spruce (Picea mariana (Mill.) B.S.P.). A panel of 22 diverse Norway spruce genotypes was screened for variation that could be observed directly using standard agarose gel electrophoresis, without additional manipulation of amplification products. Examination of marker segregation among haploid megagametophytes revealed that nine markers behaved in a codominant manner, two markers had codominant length polymorphisms and null alleles, and four others had dominant length polymorphisms. DNA sequencing of codominant alleles at seven loci indicated that most insertions/deletions (indels) were in noncoding regions and that alleles often differed by the presence or absence of direct repeats that ranged in size from three to 23 bp. The nine markers that showed ...

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Richa Agarwala and Alejandro Schäffer developed software, called rh_tsp_map, to construct radiation hybrid maps and to integrate maps that contain overlapping marker sets. Many improvements in version 3.0 of rh_tsp_map were implemented by Edward Rice. He is also first author of an extensive tutorial and set of man pages that now accompany the rh_tsp_map download shown as a link on the left. The radiation hybrid mapping methods are based on: a new strategy to select framework markers, a known reduction from the radiation hybrid mapping problem to the traveling salesman problem, and using the existing software CONCORDE to solve large instances of the traveling salesman problem. The map construction software was used at NCBI to construct dense human radiation hybrid maps. Follow the link on the right to learn more about these maps. The software has also been used to construct maps of the cat and the dog, which are described in some of the references, as well as other vertebrates. Participants ...

Richa Agarwala and Alejandro Schäffer developed software, called rh_tsp_map, to construct radiation hybrid maps and to integrate maps that contain overlapping marker sets. Many improvements in version 3.0 of rh_tsp_map were implemented by Edward Rice. He is also first author of an extensive tutorial and set of man pages that now accompany the rh_tsp_map download shown as a link on the left. The radiation hybrid mapping methods are based on: a new strategy to select framework markers, a known reduction from the radiation hybrid mapping problem to the traveling salesman problem, and using the existing software CONCORDE to solve large instances of the traveling salesman problem. The map construction software was used at NCBI to construct dense human radiation hybrid maps. Follow the link on the right to learn more about these maps. The software has also been used to construct maps of the cat and the dog, which are described in some of the references, as well as other vertebrates. Participants ...

Once a tiling path has been found, the BACs that form this path are sheared at random into smaller fragments and can be sequenced using the shotgun method on a smaller scale.. Although the full sequences of the BAC contigs is not known, their orientations relative to one another are known. There are several methods for deducing this order and selecting the BACs that make up a tiling path. The general strategy involves identifying the positions of the clones relative to one another and then selecting the least number of clones required to form a contiguous scaffold that covers the entire area of interest. The order of the clones is deduced by determining the way in which they overlap.[15] Overlapping clones can be identified in several ways. A small radioactively or chemically labeled probe containing a sequence-tagged site (STS) can be hybridized onto a microarray upon which the clones are printed.[15] In this way, all the clones that contain a particular sequence in the genome are identified. ...

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The molecular marker analysis positioned each deletion breakpoint relative to a defined region on the current MGD/CCR genetic map. This analysis did not identify deletions in addition to the previously characterized 17Pub with breakpoints useful for further refining the ∼0.8-cM functional interval associated with perturbed mesoderm development leading to midgestational lethality of the 1Acrg mutant embryo (Welsh and OBrien 2000). However, a 1.4-Mb BAC contig has been assembled over this critical region and is being used for the identification of candidate genes (Kuriharaet al. 2000). Several of the deletion breakpoints were positioned within the previously characterized functional intervals associated with genes that are essential for newborn survival and normal skeletal and CNS development. In these regions, all of the available D14Mit SSLP markers or STS markers derived from BAC ends were used to construct higher resolution maps (Figures 2 and 3). The ordering of the breakpoints within ...

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SageCode =, print 1+2, # This is the default code if none given. ButtonText =, Start/Restart the Interactive Cell, CellServer =, http://sagecell.sagemath.org, SageAnswerName =, sageAnswer, # not used yet SageAnswerValue =, ansList, # not used yet AutoEvaluateCell =, true, # false requires student to activate cell ShowAnswerBlank =, hidden, # Set to input to see Sage answer AnswerReturn =, 1, # Set to 0 if Sage returns nothing HideElements =, [], # List of items to hide in cell LinkedCells =, false, # To allow for sharing between multiple cells ...

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