Septins are guanosine-5-triphosphate-binding proteins involved in wide-ranging cellular processes including cytokinesis, vesicle trafficking, membrane remodelling and scaffolds, and with diverse binding partners. Precise roles for these structural proteins in most processes often remain elusive. Identification of small molecules that inhibit septins could aid in elucidating the functions of septins and has become increasingly important, including the description of roles for septins in pathogenic phenomena such as tumorigenesis. The plant growth regulator forchlorfenuron, a synthetic cytokinin known to inhibit septin dynamics, likely represents an informative probe for septin function. This report deals with septins of the human blood fluke Schistosoma mansoni and their interactions with forchlorfenuron. Recombinant forms of three schistosome septins, SmSEPT5, SmSEPT7.2 and SmSEPT10, interacted with forchlorfenuron, leading to rapid polymerization of filaments. Culturing developmental stages ...

Septin proteins are necessary for cytokinesis in budding yeast and Drosophila and are thought to be the subunits of the yeast neck filaments. To test whether septins actually form filaments, an immunoaffinity approach was used to isolate a septin complex from Drosophila embryos. The purified complex is comprised of the three previously identified septin polypeptides Pnut, Sep2, and Sep1. Hydrodynamic and sequence data suggest that the complex is composed of a heterotrimer of homodimers. The complex copurifies with one molecule of bound guanine nucleotide per septin polypeptide. It binds and hydrolyzes exogenously added GTP. These observations together with conserved sequence motifs identify the septins as members of the GTPase superfamily. We discuss a model of filament structure and speculate as to how the filaments are organized within cells. ...

Septins certainly are a category of conserved proteins that form hetero-oligomeric complexes that assemble into filaments. septin filaments in the medial cortex in fission candida. These insights should aid understanding of the organization and function of the varied septin constructions in animal cells. Intro Genes that encode septins were identified first more than 30 years ago in budding candida (cells communicate seven septins, five of which are involved in mitosis (explained below). Homologous genes have been recognized and characterized in many additional eukaryotic varieties [2,3]. The genome of encodes five septins, two and spp.) encode septin orthologs, but higher vegetation do not. Similarly, the genomes of protozoa, additional eukaryotic protists ((a budding microbe that harbors a membrane-bounded nucleoid, but is definitely classified with the bacterial phyla based on the majority of its recognizable genes) lacks identifiable septins. Table 1 Classification of septins in several ...

Post-translational modifications of septins are described in many different organisms from yeast to mammals. The most common modification is a phosphorylation, which has been shown to have an effect on septin dynamics (Tang and Reed 2002; Versele and Thorner 2004; Garcia et al. 2011; Sinha et al. 2007; Meseroll et al. 2012, 2013; Amin et al. 2008; Koch et al. 2015). In this work, we discovered that Drosophila septin Pnut is phosphorylated during early embryogenesis. Mass spectrometry has identified that the phosphorylation occurs at the extreme C-terminus of the protein on amino acids T509 and S517. One of these phosphorylation sites (S517) was also found during mass spectrometry-based phosphoproteome analysis of Drosophila embryos; however, no functional studies were performed (Zhai et al. 2008).. Members of the septin family vary greatly in terms of the sequence and length of their N- and C-termini (Hall and Russell 2004). In the crystal structure of the human SEPT2/6/7 complex (Sirajuddin et ...

Both the sec6/8 complex and septin filaments have been implicated in directing vesicles and proteins to sites of active membrane addition in yeast. The rat brain sec6/8 complex coimmunoprecipitates with a filament composed of four mammalian septins, suggesting an interaction between these complexes. …

The septins are a class of GTP-binding cytoskeletal proteins conserved throughout eukaryotes. They were first described as a set of cell division cycle mutants in the budding yeast Saccharomyces cerevisiae, but since their initial discovery septins have been found to participate in a variety of cellular functions, including scaffolding of other signalling proteins and acting as membrane diffusion barriers. Misexpression or dysfunction of septins in humans is associated with neurodegenerative diseases and cancer. Septins polymerize into heteromeric complexes and form a number of different cortically-associated higher-order structures, such as rings and bars, in cells. Although it is evident that the ability of septins to form these structures is tightly linked to their functionality, the precise mechanisms governing septin structure organization remain mysterious. This thesis addresses some of the questions surrounding septin complex formation and their assembly into the array of higher order ...

Yeast and mammalian septins associate with biological membranes through a highly conserved polybasic region at the N-terminus of the GTP-binding domain (Fig. 2A). Through this region, recombinant yeast septins associate preferentially with phosphatidylinositol (4)-phosphate [PtdIns(4)P] and phosphatidylinositol (5)-phosphate [PtdIns(5)P], whereas recombinant SEPT4 specifically binds phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P2] and phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5)P3] (Casamayor and Snyder, 2003; Zhang et al., 1999). In yeast cells that exhibit defective PtdIns(4)P synthesis, septins fail to assemble properly at the mother-bud neck (Casamayor and Snyder, 2003). In mammalian cells, sequestration of PtdIns(4,5)P2 and reduction of its overall level at the membrane leads to the loss of SEPT4 filaments (Zhang et al., 1999), and lipid-based signaling events and PtdIns(4,5)P2-mediated regulation of the actin cytoskeleton might indirectly influence septin ...

Another recent study (6) independently discovered in human Sept9 two predicted AHs, one adjacent to (partially overlapping) the known polybasic motif, and another next to a previously uncharacterized polybasic motif. The polybasic motifs turned out to be important for septin polymerization, and it was the AHs that mediated membrane association. Yet another recent study (7) exploited synthetic vesicles and wavy membrane surfaces to come to essentially the same conclusions as Cannon et al. (1) in terms of yeast septin filament length/orientation/packing and curvature preference. The new vesicle experiments provide evidence supporting much older observations (8) that septin binding can induce membrane curvature, hinting that septins may be not just sensors but also sculptors of curved membranes. Finally, Beber et al. derive a mathematical model that may reconcile a long-standing controversy in the yeast septin field regarding how to explain observed 90° rotations in septin filament orientation ...

Article Saccharomyces cerevisiae afr1 protein is a protein phosphatase 1/glc7-targeting subunit that regulates the septin cytoskeleton during mating. Glc7, the type1 serine/threonine phosphatase in the yeast Saccharomyces cerevisiae, is targeted by a...

In this project we apply shRNA mediated knock down of candidate genes, immunofluorescence- and live cell microscopy as well as in vitro assays using purified proteins.. Septins were discovered in the bakers yeast Saccharomyces cerevisiae and still today yeast serves as a prime model organism for research on septins due to its ease of genetic manipulation and the limited number of different septin subunits. We aim at understanding the mechanisms of septin filament formation and nucleotide binding by combining biochemical assays, structural biology and cell biology. Recently our laboratory presented the first crystal structure of a yeast septin (Brausemann et al. 2016) (figure 2.2). Currently we study the mechanisms of nucleotide binding and hydrolysis in yeast septins in vitro and aim at translating our results to human septins. A robust purification pipeline for yeast- and human septin rods was therefore established in our laboratory (Renz et al. 2013 and ongoing, unpublished results).. In ...

Septins are filament-forming proteins important for organizing the cortex of animal and fungal cells. In mammals, 13 septin paralogues were recently shown to assemble into core heterohexamer and heterooctamer complexes, which serve as building blocks for apolar filamentous structures that differ among cell types. To determine how tissue-specific septin paralogue expression may shape core heteromer repertoires and thereby modulate properties of septin filaments, we devised protocols to analyze native septin heteromers with distinct numbers of subunits. Our evidence based on genetically manipulated human cells supports and extends recent concepts of homology subgroup-restricted assembly into distinct categories of apolar heterohexamers and heterooctamers. We also identify a category of tetramers that have a subunit composition equivalent to an octameric building block. These atypical tetramers are prevalent in lymphocytes and neural tissues, in which octamers are abundant but hexamers are rare. ...

Glc7, the type1 serine/threonine phosphatase in the yeast Saccharomyces cerevisiae, is targeted by auxiliary subunits to numerous locations in the cell, where it regulates a range of physiological pathways. We show here that the accumulation of Glc7 at mating projections requires Afr1, a protein required for the formation of normal projections. AFR1-null mutants fail to target Glc7 to projections, and an Afr1 variant specifically defective in binding to Glc7 [Afr1(V546A F548A)] forms aberrant projections. The septin filaments in mating projections of AFR1 mutants initiate normally but then rearrange asymmetrically as the projection develops, suggesting that the Afr1-Glc7 holoenzyme may regulate the maintenance of septin complexes during mating. These results demonstrate a previously unknown role for Afr1 in targeting Glc7 to mating projections and in regulating the septin architecture during mating. ...

Eukaryotic cells clearly monitor and respond to variations in the organization of the cytoskeleton, but the precise sensors that are used are not well understood. In yeast cells, septin proteins must be organized into filaments in a ring-like structure at a site where a bud forms and where cytokinesis eventually pinches off the daughter cells. If septins are not properly assembled, a checkpoint mechanism is engaged that delays progression of the cell division cycle so that cells do not undergo mitosis. The protein kinase Hsl1 is activated in cells with properly assembled septins and transmits a signal that promotes entry into mitosis. Hanrahan and Snyder now report that this kinase itself appears to function as a sensor of septin status. They show that, like many kinases, Hsl1 contains an autoinhibitory domain that binds to and inhibits activity of the catalytic domain. Two-hybrid analysis showed that this same kinase inhibitory domain also contains multiple septin-binding domains. In vitro, ...

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).

The septins are a conserved family of proteins that have been proposed to carry out diverse functions. In budding yeast, the septins become localized to the site of bud emergence in G1 but have not been thought to carry out important functions at this stage of the cell cycle. We show here that the septins function in redundant mechanisms that are required for formation of the bud neck and for the normal pattern of cell growth early in the cell cycle. The Shs1 septin shows strong genetic interactions with G1 cyclins and is directly phosphorylated by G1 cyclin-dependent kinases, consistent with a role in early cell cycle events. However, Shs1 phosphorylation site mutants do not show genetic interactions with the G1 cyclins or obvious defects early in the cell cycle. Rather, they cause an increased cell size and aberrant cell morphology that are dependent upon inhibitory phosphorylation of Cdk1 at the G2/M transition. Shs1 phosphorylation mutants also show defects in interaction with the Gin4 kinase, which

3. Functional analysis of SEPTIN9 isoforms. Septins are a family of filament forming proteins. Septin filaments, which are referred to as the fourth part of the cytoskeleton, consist of repeats of heteromeric octamers. Several Septins, are associated with a variety of tumors. This is particularly true for Septin9, which surprisingly seem to have both proto-oncogenic and tumor suppressor function. This dualistic function might be connected to different SEPTIN9 isoforms that have been shown to differentially direct the association of Septin filaments with microtubules and acting filaments. We try to elucidate the impact of SEPTIN9 isoforms on the cellular localization and cell stability.. ...

Members of the septin gene family are highly homologous and have been found in several species of fungi and animals where they play critical roles in cytokinesis and cell surface organization (reviewed by Field and Kellogg 1999). Interestingly, septins have not been reported in algae or land plants. It seems most likely that septins arose in a common ancestor of fungi and animals after divergence of the green plants (Wainrightet al. 1993). It is also possible that septins arose in an earlier common ancestor shared by green plants, fungi, and animals and were later lost in the green plant lineage by nonorthologous gene displacement or lineage-specific gene loss. Regardless of the evolutionary path, it is not surprising that septins are present in animals and fungi and absent in plants in view of the major differences in division mechanisms. Animal and fungal cells divide centripetally; that is, the division furrow progresses from the cortex to the interior. Plant cells, however, divide ...

Septins polymerize into heterooligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation. Filaments are assembled from asymmetrical heterotrimers, composed of SEPT2, SEPT6 and SEPT7 that associate head-to-head to form a hexameric unit. Within the trimer, directly interacts with SEPT6, while interaction with SEPT2 seems indirect. In the absence of SEPT6, forms homodimers. Interacts directly with CENPE and links CENPE to septin filaments composed of SEPT2, SEPT6 and SEPT7. Interacts with SEPT5 and SEPT8 (By similarity). Interacts with SEPT9 and SEPT11. Component of a septin core octomeric complex consisting of SEPT12, SEPT7, SEPT6 and SEPT2 or SEPT4 in the order 12-7-6-2-2-6-7-12 or 12-7-6-4-4-6-7-12 and located in the sperm annulus; the SEPT12:SEPT7 association is mediated by the respective GTP-binding domains (PubMed:25588830 ...

The septins certainly are a grouped category of GTPase enzymes necessary for cytokinesis and are likely involved in exocytosis. the -synuclein-positive cytoplasmic inclusions of Parkinsons disease, dementia with Lewy systems and multiple program atrophy [21]. Sept5 interacts with Parkin, an Electronic3 ubiquitin-protein ligase implicated in autosomal recessive familial Parkinsons disease, marketing Sept5 degradation [22]. Sept5 overexpression in the mind induces selective dopamine neurodegeneration and inhibits dopamine secretion [23]. Three septins have already been associated with severe myeloid leukaemia [Sept5, Sept6 (septin 6) and Sept9 (MSF, also known as E-septin or Ov/Br)] by fusion using the MLL gene [24,25]. Four septins, Sept2, Sept4, Sept1 (Diff6) and Sept7 (cdc10), are located in neurofibrillary tangles in post-mortem mind from patients suffering from Alzheimers disease [26], recommending that septins may possess a function within the aetiology of neuronal disease. Sept5 and ...

A tight ring-shaped structure that forms in the division plane at the site of cytokinesis; composed of members of the conserved family of filament-forming proteins called septins as well as septin-associated proteins. This type of septin structure is obse…

Cells of the budding yeast Saccharomyces cerevisiae select a site for polarized growth in a specific pattern depending on their cell type. Haploid a and α cells bud in the axial budding pattern, which requires assembly of a landmark that includes the Bud4 protein. To understand how an axial bud site is established, we performed a structure-function analysis of Bud4. Bud4 contains DUF1709 (domain of unknown function), which is similar to a part of the anillin-homology domain, and a putative Pleckstrin homology (PH) domain near its C terminus. While its localization depends on septins, a conserved family of GTP-binding proteins, Bud4 is necessary for the stable inheritance of septin rings during cell division. Although some anillins interact directly with septins, we find that neither DUF1709 nor the PH domain is necessary for targeting Bud4 to the mother-bud neck. Instead, this C-terminal region is critical for association of Bud4 with Bud3 and other components of the axial landmark. Remarkably, ...

This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimers disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012 ...

Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced ...

Septins are guanosine-5′-triphosphate-binding protein involved with wide-ranging cellular procedures including cytokinesis vesicle trafficking membrane remodeling and scaffolds and with diverse binding companions. FCF. Recombinant types of 3 schistosome septins septins that have received the real brands septins in vitro. Furthermore putative stabilization of septin polymers inside the schistosome correlated with the motility of its developmental ...

Hybrid genes are created by trans-splicing, sense/antisense transcription, genome rearrangement, or intergenic splicing between two genes [1-5]. The creation of new genes is a potential risk factor for tumor development, yet may also promote diversification by inducing gene substitution, translocation, inversion, or rearrangement [1, 6-8]. As a result, hybrid genes have the ability to be either harmful or advantageous to humans [9, 10].. Cancer genes have somatic mutations similar to chromosomal translocations that result in hybrid transcripts by apposing one gene to the regulatory regions of another. For example, a hybrid transcript is created by genomic rearrangement of the mixed-lineage leukemia (MLL) gene at 11q23 and the septin family (SEPT6) gene at Xq24 in acute leukemia patients [11]. These rearrangements result in fusions with at least 40 other genes, resulting in expression of hybrid proteins with leukemogenic activity [12, 13]. Occasionally hybrid gene formations can also contribute ...

Septin 4 is encoded by the SEPT4 gene in humans and is expressed at high levels in the brain and heart. It is a filament-forming GTPase that is localized to the cytoskeleton. Septin 4 may also play a role in cytokinesis. Septin 4 exists as several isoforms, one of which is found in mitochondria and is implicated in apoptosis and cancer. Alternate names for septin 4 include apoptosis-related protein in the TGF-beta signaling pathway (ARTS), bradeion beta, brain protein H5, H5, MART, SEP4, cell division control-related protein 2, cerebral protein 7, CE5B3, CE5B3 beta, Peanut-like protein 2 (PNUTL2), hucep-7, hCDCREL-2, and septin-M.. Septin 7 is highly similar to yeast CDC10, Drosophila Diff 6, and mouse H5 protein. Each of these proteins contains a GTP-binding motif. It functions in gliomagenesis and in the suppression of glioma cell growth. It is required for the association of centromere-associated protein E with the kinetochore during mitosis. Alternative splicing results in multiple isoforms ...

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion.

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 ...

Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress ...

Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells ...

CDC11 is a component of Septins. Septins are GTPases involved in cytokinesis. They assemble early in the cell cycle as a patch at the incipient bud…

The idea for the glass bead experiment came from many rich intellectual discussions with other members of the MBL community, says Bridges, who has accompanied Gladfelter to the MBL each summer since 2012. Both our collaborations and the imaging resources at MBL were central to this work.. ###. Citation:. Bridges, A. A., Jentzsch, M.S., Oakes, P.W., Occhipinti, P., and Gladfelter, A.S. (2016) Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton. J Cell Biol 213:23-32, doi:10.1083/jcb.201512029.. The Marine Biological Laboratory (MBL) is dedicated to scientific discovery - exploring fundamental biology, understanding marine biodiversity and the environment, and informing the human condition through research and education. Founded in Woods Hole, Massachusetts in 1888, the MBL is a private, nonprofit institution and an affiliate of the University of Chicago.. ...

Ubiquitin-protein Ligase (E3); Controls Septin Dynamics, Spindle Position Checkpoint (SPOC) With Ligase Dma2p By Regulating Recruitment Of Elm1p To Bud Neck; Regulates Levels Of EIF2 Subunit Gcd11p, As Well As Abundance, Localization, And Ubiquitination Of Cdk Inhibitory Kinase Swe1p; Ubiquitinates Cyclin Pcl1p; Ortholog Of Human RNF8, Similar To Human Chfr; Contains FHA, RING Fingers; DMA1 Has A Paralog, DMA2, That Arose From The Whole Genome Duplication

ARTS is an unusual septin-like mitochondrial protein that was originally shown to mediate TGF-beta-induced apoptosis. Recently, we found that ARTS is also important for cell killing by other pro-apoptotic factors, such as arabinoside, etoposide, staurosporine and Fas. In Drosophila, the IAP antagoni …

Sept: Meaning of Sept . What does Sept mean? Everything name meaning, origin, pronunciation, numerology, popularity and more information about Sept at NAMEANING.NET

PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.

Complete information for SEPT3 gene (Protein Coding), Septin 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Complete information for SEPT3 gene (Protein Coding), Septin 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Reaktivität: Human, Affe, Maus and more. 68 verschiedene SEPT5 Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!

David Wright didnt want to go out at all. But at least hell be able to go out his way when he suits up in a Mets uniform for the last time on Sept. 29 at Citi Field. Wright will be activated on Sept

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Protein Involved In Bud-site Selection; Required For Axial Budding Pattern; Localizes With Septins To Bud Neck In Mitosis And May Constitute An Axial Landmark For Next Round Of Budding

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Hereditary neuralgic amyotrophy is a type of nervous system disease that affects the brachial plexus. Common signs and symptoms include episodes of severe pain and muscle wasting in one or both shoulders and arms. Attacks may be spontaneous or triggered (e.g., by exercise, childbirth, surgery, infection etc.). Secondary complications, such as decreased sensation, abnormal sensations (e.g., numbness and tingling), chronic pain, and impaired movement may develop overtime. Affected members in some families may share additional distinct physical and facial characteristics. Hereditary neuralgic amyotrophy can be caused by mutations in the SEPT9 gene. It is inherited in an autosomal dominant fashion ...

Autophagy is a conserved cellular degradation pathway wherein double-membrane vesicle called autophagosomes capture long-lived proteins and damaged or superfluous organelles and deliver them to the lysosome for degradation. Septins are conserved GTP-binding proteins involved in many cellular processes, including phagocytosis and autophagy of intracellular bacteria, but no role in general autophagy was known. In budding yeast, septins polymerize into ring-shaped arrays of filaments required for cytokinesis. In an unbiased genetic screen and in subsequent targeted analysis, we found autophagy defects in septin mutants, and co-localized septins in rings at the pre-autophagosomal structure (PAS) and on autophagosomes where they physically interact with the autophagy proteins Atg8 and Atg9 ...

Anti-endothelial-cell antibodies are associated with psychiatric manifestations in systemic lupus erythematosus (SLE).Our primary aim in this study was to seek and characterize molecules that behave as endothelial autoantigens in SLE patients with psychiatric manifestations. By screening a cDNA library from human umbilical artery endothelial cells with serum from an SLE patient with psychosis, we identified one positive strongly reactive clone encoding the C-terminal region (C-ter) of Nedd5, an intracytoplasmatic protein of the septin family. To evaluate anti-Nedd5 serum immunoreactivity, we analyzed by ELISA specific IgG responses in 17 patients with SLE and psychiatric manifestations (group A), 34 patients with SLE without psychiatric manifestations (group B), 20 patients with systemic sclerosis, 20 patients with infectious mononucleosis, and 35 healthy subjects. IgG specific to Nedd5 C-ter was present in 14 (27%) of the 51 SLE patients. The mean optical density value for IgG immunoreactivity ...

TY - JOUR. T1 - SEPT12-Microtubule complexes are required for sperm head and tail formation. AU - Kuo, Pao Lin. AU - Chiang, Han Sun. AU - Wang, Ya Yun. AU - Kuo, Yung Che. AU - Chen, Mei Feng. AU - Yu, I. Shing. AU - Teng, Yen Ni. AU - Lin, Shu Wha. AU - Lin, Ying Hung. PY - 2013/11/7. Y1 - 2013/11/7. N2 - The septin gene belongs to a highly conserved family of polymerizing GTP-binding cytoskeletal proteins. SEPTs perform cytoskeletal remodeling, cell polarity, mitosis, and vesicle trafficking by interacting with various cytoskeletons. Our previous studies have indicated that SEPTIN12+/+/+/- chimeras with a SEPTIN12 mutant allele were infertile. Spermatozoa from the vas deferens of chimeric mice indicated an abnormal sperm morphology, decreased sperm count, and immotile sperm. Mutations and genetic variants of SEPTIN12 in infertility cases also caused oligozoospermia and teratozoospermia. We suggest that a loss of SEPT12 affects the biological function of microtublin functions and causes ...

A coated substrate and a method of forming a diffusion barrier coating system between a substrate and a MCrAl coating, including a diffusion barrier coating deposited onto at least a portion of a substrate surface, wherein the diffusion barrier coating comprises a nitride, oxide or carbide of one or more transition metals and/or metalloids and a MCrAl coating, wherein M includes a transition metal or a metalloid, deposited on at least a portion of the diffusion barrier coating, wherein the diffusion barrier coating restricts the inward diffusion of aluminum of the MCrAl coating into the substrate.

a IF of FA proteins paxillin in control, SEPT9-KD and SEPT9-Ov cells. IF of SEPT9_i1 (red) and paxillin (green) in SEPT9-Ov cells. b Quantification of FAs and average FA size per nucleus (*p , 0.05, vs control group; △p , 0.05, vs Knockdown group). c Interaction between SEPT9_i1 and paxillin by COIP. d Western blot confirming the inhibition in the expression of paxillin in knockdown group and overexpression of SEPT9_i1 promoting the expression of paxillin (*p , 0.05, ***p , 0.001) ...

Thank you for sharing this Journal of Virology article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...

Geisinger Wyoming Valley Medical Center Announces Newborns for the days of September 23 to Oct. 13, 2017. Lindsay, Melinda and Edwin, Scranton, a daughter, Sept. 23. Linden, Rachel and Mike, Wilkes-Barre, a daughter, Sept. 24. Briggs, Aaleeyah and Wade, Gary, Edwardsville, a daughter, Sept. 25. Melendez, Crystalee and Jimenez, Jean Serrano, Kingston, a son, Sept. 25. Johnson, Alexis and Darby, Johnny, Edwardsville, a daughter, Sept. 25. Connolly, Kaitlyn and VanWhy, David, Wyoming, a daughter, Sept. 25. Skoniecki, Mackenzie and Waligun, Jaggar, Plymouth, a daughter, Sept. 25. Shuman, Amanda and Lamoreaux, Lloyd, Hunlock Creek, a daughter, Sept. 26. Sikora, Autumn and Adams, Jason, Huntington Mills, a son, Sept. 26. Graham, Caitlin and Joseph, Waverly, a daughter, Sept. 26. Miller, Stephanie and Jeff, Tunkhannock, a son, Sept. 27. Gross, Valerie and Bevans, John, Hazle Township, a son, Sept. 27. Drevenak, Kimberly and Andrew, Hanover Township, a daughter, Sept. 27. Dias, Kendra Leigh and Davis, ...

Introduction Altered expression of Septin 9 ( SEPT9), a septin coding for multiple isoform variants, has been observed in several carcinomas, including colorectal, head and neck, ovarian and breast,...

Mates L، Korpos E، Deak F، Liu Z، Beier DR، Aszodi A، Kiss I (Mar 2002). Comparative analysis of the mouse and human genes (Matn2 and MATN2) for matrilin-2, a filament-forming protein widely distributed in extracellular matrices. Matrix Biol. 21 (2): 163-74. PMID 11852232. doi:10.1016/S0945-053X(01)00194-9. ...

Blog on SEPT5 positive control product: The SEPT5 sept5 (Catalog #MBS543230) is a Positive Control and is intended for research purposes only. Th...

Sept12 - mouse gene knockout kit via CRISPR, 1 kit. |dl||dt|Kit Component:|/dt||dd|- |strong|KN315528G1|/strong|, Sept12 gRNA vector 1 in |a href=http://www.origene.com/CRISPR-CAS9/Detail.

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Aulia, Septin Ambar and , Imron Rosyadi, S.E, M.Si. (2016) Pengaruh Pendidikan, Pelatihan Dan Motivasi Kerja Terhadap Produktivitas Kerja Karyawan (Pada Pt. Inti Sukses Garmindo,Semarang). Skripsi thesis, Universitas Muhammadiyah Surakarta. ...

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Go to a pop stars concert, play a game of D&D, attend an art exhibit opening or enjoy the outdoors among a field of sunflowers.

The Pentagon will require members of the U.S. military to get the COVID-19 vaccine by Sept. 15, according to a memo obtained by The Associated Press. That deadline could be pushed up if the vaccine receives final FDA approval or infection rates continue to rise.

The city must make a $300,000 principal payment by Sept. 2 toward the $11.9 million it borrowed in 2005 to help fund the construction of the Covelli Centre.

View Notes - CH310-318 HW2key_1 from CH 54410 at University of Texas. Sessler CH310M/318M Homework Problem Set 2 310M Due Wednesday Sept. 17 318M 310M TIPS Circle the section in which you are

There will be a spectacular total lunar eclipse on the night of Sept. 27-28, 2015, a newly dubbed Mega Hemorrhaging Eclipse. Here is info on what lunar eclipses are and how to observe the eclipse.

The Shadowland Theatre of Ellenville, New York, ends its revival of British actress Shelagh Stephenson's The Memory of Water Sept. 12 The third US production of the play began performances Aug. 27. Bill Lelbach directs.

Customized TV Listings are available here: www.latimes.com/tvtimes Click here to download TV listings for the week of Sept. 1 - 7, 2013 in PDF format This weeks TV Movies SUNDAY

Class Preparation Advanced ReadingSilent Waves, All of Part 1, All of 2; in Part 5 just chapter one and all of Part 6.Lymph Drainage Therapy 1 Anatomical TermsIn order to be best prepared for LDT1, be sure you clearly understand the following words and, as applicable, know where to locate them in the body:• Proximal, distal, medial, lateral, anterior, posterior, superior (cephalic), inferior (caudal)• Acute, subacute• Afferent, efferent vessel

Hey ladies, Im ttc #1 & Im CD8 so Ill be testing the first week of Sept, anyone else trying this month & testing next too? Im [...] - Poster:Maggy S - Page: 62 - Pages:344 - Last post08-12... Read more on Netmums

TERA: Rising developer Bluehole Studio will deliver the games next major update, Dungeon Assault, on Sept. 10, bringing four new dungeons to the action MMO, publisher En Masse Entertainment...

Satterthwaite, Mark, Steven R. Williams, and Konstantinos E. Zachariadis. 2018. Price Discovery Using a Double Auction, Sept. 17..

Buy tickets to PG Pizza Night (Meet the Mentors) (Postgraduates) on Thursday 23 Sept at E41, Portland Building, University Park, Portland Building, University Park, Nottingham, | FIXR

Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.

|strong|SEPT. 14 to SEPT. 20|/strong| When then-county commissioner Chen Ding-nan (陳定南) announced that movie theaters in Yilan County no longer needed to play the national anthem before each showing, the authorities were displeased. It was Sept. 13, 1988, over a year after the lifting of martial law, but the decades-old tradition where moviegoers had to stand and sing the anthem still endured. Of course, Chen sugarcoated his decision:

Inmitten bezaubernder, grüner Landschaft liegt unser Sportlerhof (528 m SH). Unser neuer, baubiologischer Zubau umfasst eine Ferienwohnung für 2-5 Personen, ein Familienzimmer für 2-4 Personen und ein Zimmer für 2-3 Personen. Diese sind mit Vollholzmöbeln ausgestattet, die Elternschlafzimmer mit Zirbenholz, Dusche/WC extra, und mit sehr viel Liebe zum Detail geschmückt. Der südseitige Balkon mit Glasüberdachung bzw. eine Terrasse bei jeder Wohnung laden zum gemütlichen Entschleunigen ein. Brötchenservice gerne zubuchbar, Frühstück auf Anfrage im Vorhinein! Ab drei Nächten Salzkammergut Card gratis!. HERBSTANGEBOT Waldnessangebote: 29. Aug. bis 01. Sept./01. bis 04. Sept./05. bis 08. Sept./ 03. bis 06. Okt./ 06. bis 09. Okt./ 10. bis 13. Okt. 2019. Auf geht´s zur 1. Waldnessdestination Frauen-Power-Entdeckungsreise -buchen Sie Ihren Mädelstrip, Mama-Tochter Ausflug, Freundinnen-Stammtisch etc. - Sie werden beeindruckt sein! Wald wirkt wirklich!Verbringen Sie mit Ihren liebsten ...

THE EXOTIC CAT CLUB OF QLD Inc. SEPT 26th 2021 [COVID SAFE EVENT - QLD GOVT. COVID RULES]. Exotic schedule SEPT - PDF Exotic schedule SEPT 21 - WORD. ...

InstituteiHumanInstituteResearchAreaCellBiologyContactxufei@shanghaitech.edu.cn,WebpageBiography-Sept2013-presentResearchAssociateProfessor,iHumanInstitute,ShanghaiTechUniversityFocusedonGPCRsignalinginstemcelldevelopmentandcancer-Sept2012-Sept2013ScientificConsultant,RuiYiInc.,LaJolla,CA,USA-Aug2011-Sept2012FounderandDirector,RDRuiYiBiopharmaceuticsTechnologyCo.,Ltd.,Shanghai,ChinaFocusedonbiologicdrugdevelopmenttargetingonGPCR-July2006-June2011Ph.D.inBiologyTheScrippsResearchInstitute(TSRI),La

I felt it was time to do another life update vlog for anyone curious whats going on in my life right now, and it turned out I have a lot to say, so I broke it up into three parts approximately by category. All three parts are embedded below in the order that they were filmed, so if you want a comprehensive idea of everything Ive got going on, you can watch them in order ...