Variations in free secretory component levels in mucosal secretions of the rat. | The Journal of Immunology
The present studies were conducted to compare the levels of free secretory component (SC) in a number of rat mucosal secretions and to determine whether SC content varies significantly during the four stages of the estrous cycle. Levels of SC, as measured by radioimmunoassay, were markedly different in various external secretions. Bile contained the highest amount, irrespective of whether SC was normalized to volume or protein. Concentrations of SC in saliva or uterine fluid from intact rats were approximately 20- to 30-fold less than measured in bile. When SC levels were normalized to protein, the SC to protein ratios in uterine, vaginal, and respiratory secretions were six to 18 times greater than values calculated in salivary and small intestinal fluids. Analysis of SC levels in mucosal secretions during the estrous cycle indicated significant variations occur in uterine and vaginal samples, but not in saliva or small intestinal secretions. In the uterine lumen, SC levels were highest at ...
Université Francois Rabelais - Tours - Human eosinophils express a receptor for secretory component. Role in secretory IgA...
The existence of a functional receptor for secretory component (SC) on the eosinophil membrane might explain the preferential degranulation induced by secretory IgA (sIgA) when compared to serum IgA. Indeed, flow cytometry analysis revealed that purified human SC could bind to a subpopulation (4-59%) of blood eosinophils purified from 19 patients with eosinophilia. Binding of radiolabeled human SC could be competitively inhibited using unlabeled SC or secretory IgA but not with serum IgA or IgG. Immunoprecipitation and immunosorbent chromatography using human SC revealed the presence of a major component at 15 kDa in eosinophil extracts as well as in culture supernatants but not in neutrophils. The 15-kDa protein eluted from the human SC immunosorbent was able to bind to SC or to sIgA but not to serum IgA. Eosinophils preincubated with human SC or sIgA released eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) after addition of anti-SC or anti-IgA monoclonal antibody as respective cross
ReP USP - Detalhe do registro: Immunoassays of secretory iga and secretory component
NAGAO, A T; RAMOS, O L; PEREIRA, Arnaldo Bueno. Immunoassays of secretory iga and secretory component. Brazilian Journal of Medical and Biological Research, Ribeirao Preto, v. 23, p. 211-24, 1990 ...
IgA Secretory Component / ECM1 Antibody - With BSA and Azide - Purified Mouse Monoclonal Antibody WB, IHC, IF, FC, IP, E - Buy...
IgA Secretory Component / ECM1 Antibody - With BSA and Azide, Purified Mouse Monoclonal Antibody validated in WB, IHC, IF, FC, IP, E (AH10327-20), Abgent
ECM-1/Secretory Component P85 Antibody (SPM217) [Allophycocyanin] (NBP2-34747APC): Novus Biologicals
Mouse Monoclonal Anti-ECM-1/Secretory Component P85 Antibody (SPM217) [Allophycocyanin]. Validated: Flow. Tested Reactivity: Human, Rat. 100% Guaranteed.
Blood component production - Stock Image C004/0021 - Science Photo Library
Blood component production. Technician with a bioreactor used for producing blood components. Bioreactors are devices that maintain a specific environment to allow organisms or their derivative substances to undergo chemical processes. Here it is being used to culture various blood components such as plasma proteins, growth and coagulation factors, albumin, and immunoglobulin. Photographed at the Laboratoire de Fractionnement et des Biotechnologies (Laboratory of Fractionation and Biotechnology, LFB), Paris, France. - Stock Image C004/0021
Peroxisome proliferator-activated receptor γ level contributes to structural integrity and component production of elastic...
TY - JOUR. T1 - Peroxisome proliferator-activated receptor γ level contributes to structural integrity and component production of elastic fibers in the aorta. AU - Tai, Haw Chih. AU - Tsai, Pei Jane. AU - Chen, Ju Yi. AU - Lai, Chao Han. AU - Wang, Kuan Chieh. AU - Teng, Shih Hua. AU - Lin, Shih Chieh. AU - Chang, Alice Y.W.. AU - Jiang, Meei Jyh. AU - Li, Yi Heng. AU - Wu, Hua Lin. AU - Maeda, Nobuyo. AU - Tsai, Yau Sheng. PY - 2016/6/1. Y1 - 2016/6/1. N2 - Loss of integrity and massive disruption of elastic fibers are key features of abdominal aortic aneurysm (AAA). Peroxisome proliferator-activated receptor γ (PPARγ) has been shown to attenuate AAA through inhibition of inflammation and proteolytic degradation. However, its involvement in elastogenesis during AAA remains unclear. PPARγ was highly expressed in human AAA within all vascular cells, including inflammatory cells and fibroblasts. In the aortas of transgenic mice expressing PPARγ at 25% normal levels (PpargC/- mice), we ...
Component Production Tech I (Evenings) - Houston, TX
Apply for Component Production Tech I (Evenings) position at Gulf Coast Regional Blood Center in Houston, TX on InformationTechnologyJobs.com - Job ID: 12914999
A study of the role of Rab27 in lacrimal gland acinar cell secretory trafficking :: University of Southern California...
Tear proteins are supplied by the regulated fusion of secretory vesicles with the apical surface of lacrimal gland acinar cells, utilizing trafficking mechanisms largely yet uncharacterized. We investigated the role of Rab27b in regulating the exocytotic pathway of these secretory vesicles. Evaluation of morphological changes by transmission electron microscopy of lacrimal glands from Rab27b-/- and Rab27ash/ash/Rab27b-/- mice, but not Ashen mice deficient in Rab27a, showed significant changes in organelle morphology which included an approximate 50% decrease in abundance of secretory vesicles and decreased vesicle localization in the subapical region. Along with an apparent secretory pathway effect, knockout of Rab27b also resulted in a two-fold increase in the number of lysosomes, four-fold increase number of damaged mitochondria, two-fold increase in the formation of autophagosome-like organelles, and observed increased ER swelling and vesiculation. Confocal fluorescence microscopy analysis, ...
Functional expression of the polymeric immunoglobulin receptor from cloned cDNA in fibroblasts. | JCB
The polymeric immunoglobulin receptor, a transmembrane protein, is made by a variety of polarized epithelial cells. After synthesis, the receptor is sent to the basolateral surface where it binds polymeric IgA and IgM. The receptor-ligand complex is endocytosed, transported across the cell in vesicles, and re-exocytosed at the apical surface. At some point the receptor is proteolytically cleaved so that its extracellular ligand binding portion (known as secretory component) is severed from the membrane and released together with the polymeric immunoglobulin at the apical surface. We have used a cDNA clone coding for the rabbit receptor and a retroviral expression system to express the receptor in a nonpolarized mouse fibroblast cell line, psi 2, that normally does not synthesize the receptor. The receptor is glycosylated and sent to the cell surface. The cell cleaves the receptor to a group of polypeptides that are released into the medium and co-migrate with authentic rabbit secretory ...
Recombinant Human Polymeric Immunoglobulin Receptor/PIgR (C-6His) | Bon Opus Biosciences
Recombinant Human Poly-Ig receptor is produced by our Mammalian expression system and the target gene encoding Lys19-Arg638 is expressed with a 6His tag at the C-terminus. Bon Opus Cat.# C381 (BP074)
Protein traffic in polarized epithelial cells: the polymeric immunoglobulin receptor as a model system | Journal of Cell Science
As a model system to study protein traffic in polarized epithelial cells, we have used the polymeric immunoglobulin receptor. This receptor travels first to the basolateral surface, where it can bind polymeric IgA or IgM. The receptor is then endocytosed and delivered to endosomes. The receptor is sorted into transcytotic vesicles, which are exocytosed at the apical surface. The 103-amino acid cytoplasmic domain of the receptor contains several sorting signals. The 17 residues closest to the membrane are an autonomous signal that is necessary and sufficient for basolateral sorting. For rapid endocytosis there are two independent signals, both of which contain critical tyrosine residues. Finally, transcytosis is signaled by phosphorylation of a particular serine. ...
Human pIgR mimetic peptidic ligand for affinity purification of IgM: Part I: Ligand design and binding mechanism | ScholarBank...
Synthetic peptides incorporating the complementarity determining region 2 (CDR2)-like loop of domain 1 of human polymeric immunoglobulin receptor (hpIgR-D1) were investigated for their interactions with human Immunoglobulin-M (hIgM) and other hIgs. pep14, a 14mer peptide, emerged as a unique biomimetic ligand with specificity to interact with hIgM and negligible affinity for hIgG, hIgE, hIgA1 and bovine serum albumin. Surface plasmon resonance-based binding studies between immobilized pep14 and fragments of hIgM molecule, namely Fc5μ and Fab, suggested that pep14 was binding to a motif in the constant domain 2 of hIgM. Modified peptides, pep13, pep5A and pep14A were investigated for their interaction with hIgM/ hIgG1 to elucidate the binding mechanism of pep14. Cysteine in pep14 was identified to be crucial for inducing thiophilic-like interactions between the ligand and hIgM while glutamic acid had a significant role in attributing specificity to the ligand to interact with hIgM. Circular ...
Immunoglobulin A - Wikipedia
The high prevalence of IgA in mucosal areas is a result of a cooperation between plasma cells that produce polymeric IgA (pIgA), and mucosal epithelial cells that express an immunoglobulin receptor called the polymeric Ig receptor (pIgR). pIgA is released from the nearby activated plasma cells and binds to pIgR. This results in transportation of IgA across mucosal epithelial cells and its cleavage from pIgR for release into external secretions.[13]. Production of sIgA against specific antigens depends on sampling of M cells and underlying dendritic cells, T cell activation, and B cell class switching in GALT, mesenteric lymph nodes, and isolated lymphoid follicles in the small intestine.[14]. Polymeric IgA (mainly the secretory dimer) is produced by plasma cells in the lamina propria adjacent to mucosal surfaces. It binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells, and is taken up into the cell via endocytosis. The receptor-IgA complex passes through ...
Analysis of endocytic and trafficking pathways of potential candidates for drug delivery in HeLa and lacrimal gland acinar...
When identifying potential candidates for drug delivery or biomedical diagnostic tools, it is of utmost importance to delineate, as best as possible, their uptake and intracellular trafficking pathways. Here we investigated these pathways, in a simple cell model first, followed by a more complex and physiologically-relevant cell model, for three potential theranostic and drug delivery candidates. The first is a nanoparticle based on difluoroboron dibenzoylmethane-poly(lactic acid), that exhibits unique molecular-weight dependent emission properties. We showed that these nanoparticles are highly photostable, in vitro and in situ, resisting laser-induced photobleaching under conditions that destroy the fluorescence associated with a common photostable probe, LysoTracker Blue. Also, we showed that the internalized nanoparticles do not accumulate in acidic compartments such as late endosomes and lysosomes, but rather in a non-lysosomal perinuclear compartment. Additionally, we demonstrated that ...
Most recent papers with the keyword John Zhang | Read by QxMD
Loss of secretory immunoglobulin A (SIgA) is common in the small airways of patients with chronic obstructive pulmonary disease (COPD) and may contribute to disease pathogenesis. Using mice that lack SIgA in the airways due to genetic deficiency of polymeric immunoglobulin receptor (pIgR-/- mice), we investigated the role of neutrophils in driving the fibrotic small airway wall remodeling and emphysema that develops spontaneously in these mice. By flow cytometry, we found an increase in the percentage of neutrophils among CD45+ cells in the lungs, as well as an increase in total neutrophils, in pIgR-/- mice compared to wild-type (WT) controls ...
Structural Insights into Antibody-Mediated Mucosal Immunity - CaltechAUTHORS
The mucosal regions of the body are responsible for defense against environmental pathogens. Particularly in the lumen of the gut, antibody-mediated immune responses are critical for preventing invasion by pathogens. In this chapter, we review structural studies that have illuminated various aspects of mucosal immunity. Crystal structures of IgA1-Fc and IgA-binding fragments of the polymeric immunoglobulin receptor and FcαRI, combined with models of intact IgA and IgM from solution scattering studies, reveal potential mechanisms for immune exclusion and induction of inflammatory responses. Other recent structures yield insights into bacterial mechanisms for evasion of the host immune response. ...
HT-29 | Creative Bioarray
Established from an adenocarcinoma from a 44-year-old female. Tumorigenic in nude mice and has ability to grow in agar. They have human adrenergic alpha2A urokinase receptor (u-PAR), vitamin D (moderate expression) and urokinase receptor (u-PAR); The cell line can produce secretory component of IgA, carcinoembryonic antigen (CEA), transforming growth factor beta binding protein and mucin. Ultrastructural features include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough ER with free ribosomes, lipid droplets, few primary and many secondary lysosomes. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution ...
Antibody to coliform antigens in urine samples from patients with symptoms of urinary tract infection. | Journal of Clinical...
An enzyme linked immunoadsorbent assay (ELISA) was used to detect IgG, IgM, and IgA antibody in urine to a crude antigenic mixture prepared from six arbitrarily chosen strains of common coliform urinary pathogens. In a group of patients with symptoms of urinary tract infection 24 of 80 (30%) urine samples were positive on culture by conventional methods, 58 of 80 (72.5%) were positive for polymorphs on microscopy, and 72 of 80 (90%) were positive for antibody by ELISA. Secretory component was detected in 28 of 80 (35%) samples, and this is consistent with local antibody production. The results show that antibody can be detected in a wide range of urine samples using a simple antigen mixture, and this may form the basis of a useful test to diagnose urinary tract infection.. ...
On the mechanism of unidirectional killing in mixtures of two cytotoxic T lymphocytes. Unidirectional polarization of...
In mixtures of two CTL of the type a anti-b and b anti-c, only the latter is lysed; i.e., killing is unidirectional. Here, we show that two profound types of changes occur in the effector CTL but not in the target CTL upon formation of couples between them. One is that the microtubule organizing center (and presumably the Golgi apparatus that is invariably colocalized with it) is reoriented inside the effector CTL to face the bound target CTL. This unidirectional reorientation, it is proposed, serves to direct putative cytotoxic secretory components derived from the Golgi apparatus of the effector cell to the site of cell-cell binding. The second unidirectional change is in the membrane-associated cytoskeleton of the effector CTL in the area of target cell binding. The cytoskeletal protein talin, but not any of four other such proteins assayed, is highly concentrated at the contacting membrane of the effector CTL, while it is uniformly distributed over the entire membrane of the bound target ...
Experimental IgA nephropathy in bile duct ligated rats<...
TY - JOUR. T1 - Experimental IgA nephropathy in bile duct ligated rats. AU - Melvin, Tyrone. AU - Burke, Barbara. AU - Michael, Alfred F.. AU - Kim, Youngki. N1 - Funding Information: The authors wish to thank Ms. Kathy Divine, Ms. Crystal Blocher, and Ms. Kim Pinkham for technical assistance, Mr. Marshall Hoff for the preparation of figures, and Ms. Mary Jo Jansen for typing the manuscript. This work was supported in part by grants from the NIH (AM007087) and the Viking Childrens Fund. Dr. Brian Underdown kindly provided rabbit anti-rat secretory component antibody.. PY - 1983/6. Y1 - 1983/6. N2 - Polymeric IgA and polymeric IgA-containing immune complexes are transported from blood to bile through hepatocyte-bound secretory component. In order to investigate interruption of this transport and its effect on the glomerulus, Sprague-Dawley rats underwent bile duct ligation. Renal tissue obtained at the time of sacrifice was stained by immunofluorescent techniques with antibodies to IgG, IgM, and ...
Effect of nocodazole on vesicular traffic to the apical and basolateral surfaces of polarized MDCK cells.
Author(s): Breitfeld, PP; McKinnon, WC; Mostov, KE | Abstract: A polarized cell, to maintain distinct basolateral and apical membrane domains, must tightly regulate vesicular traffic terminating at either membrane domain. In this study we have examined the extent to which microtubules regulate such traffic in polarized cells. Using the polymeric immunoglobulin receptor expressed in polarized MDCK cells, we have examined the effects of nocodazole, a microtubule-disrupting agent, on three pathways that deliver proteins to the apical surface and two pathways that deliver proteins to the basolateral surface. The biosynthetic and transcytotic pathways to the apical surface are dramatically altered by nocodazole in that a portion of the protein traffic on each of these two pathways is misdirected to the basolateral surface. The apical recycling pathway is slowed in the presence of nocodazole but targeting is not disrupted. In contrast, the biosynthetic and recycling pathways to the basolateral surface are
The Dry Eye Digest: Abstract: The peptides in our tears...
Tear fluid is a complex mixture of biological compounds, including carbohydrates, lipids, electrolytes, proteins, and peptides. Despite the physiological importance of tear fluid, little is known about the identity of its endogenous peptides. In this study, we analyzed and identified naturally occurring peptide molecules in human reflex tear fluid by means of LC-MALDI-TOF-TOF. Tandem MS analyses revealed 30 peptides, most of which have not been identified before. Twenty-six peptides are derived from the proline-rich protein 4 and 4 peptides are derived from the polymeric immunoglobulin receptor. Based on their structural characteristics, we suggest that the identified tear fluid peptides contribute to the protective environment of the ocular surface ...
Toji, JP - Patent applications
LOW-THERMAL-EXPANSION NI-BASED SUPER-HEAT-RESISTANT ALLOY FOR BOILER AND HAVING EXCELLENT HIGH-TEMPERATURE STRENGTH, AND BOILER COMPONENT AND BOILER COMPONENT PRODUCTION METHOD USING THE SAME - Disclosed is a low-thermal-expansion Ni-based super-heat-resistant alloy for a boiler, which has excellent high-temperature strength. The alloy can be welded without the need of carrying out any aging treatment. The alloy has a Vickers hardness value of 240 or less. The alloy comprises (by mass) C in an amount of 0.2% or less, Si in an amount of 0.5% or less, Mn in an amount of 0.5% or less, Cr in an amount of 10 to 24%, one or both of Mo and W in such an amount satisfying the following formula: Mo+0.5 W=5 to 17%, Al in an amount of 0.5 to 2.0%, Ti in an amount of 1.0 to 3.0%, Fe in an amount of 10% or less, and one or both of B and Zr in an amount of 0.02% or less (excluding 0%) for B and in an amount of 0.2% or less (excluding 0%) for Zr, with the remainder being 48 to 78% of Ni and unavoidable ...
Table E1). dimeric type (1:1 proportion of monomeric and Rabbit - Stimulant effects of adenosine antagonists on operant behavior
Table E1). dimeric type (1:1 proportion of monomeric and Rabbit Polyclonal to EPHB1 dimeric; Athens Technology and Research, Athens, GA) was added to the basolateral mass media. Twenty-four hours afterwards, apical washings were gathered and replicate wells were harvested for pIgR and histology gene and protein analyses. Goat polyclonal anti-SC/pIgR antibody (Ur&Chemical Systems, Minneapolis, MN) was used for West and IHC blotting. Total SIgA and IgA concentrations in apical washings were measured using particular ELISAs. Current Polymerase String Response Total RNA from microdissected bronchial epithelial cells was removed using the RNAqueous-Micro Package (Applied Biosystems/Ambion, Austin texas, Texas), regarding to the manufacturers process. Total RNA from air-liquid user interface (ALI) cultured cells was singled out using the RNeasy Mini package (Qiagen, Valencia, California), regarding to the manufacturers specs. Primer sequences had been as comes after: (Forwards ...
Anti-inflammatory role for intrac... preview & related info | Mendeley
(2003) Fernandez et al. Immunity. Intestinal epithelial cells (IEC) play a central role in innate and acquired mucosal immunity. They ensure early signaling to trigger an inflammatory response against pathogens. Moreover, IEC mediate transcytosis of dimeric IgA (dIgA), through the polymeric-immun...
Harvey R Kaslow, | Keck School of Medicine of USC
Publications. P2X7 receptor-dependent and -independent T cell death is induced by nicotinamide adenine dinucleotide. J Immunol. 2005 Feb 15; 174(4):1971-9. View in: PubMed. Expression of IL-10 and TNF-inhibitor genes in lacrimal gland epithelial cells suppresses their ability to activate lymphocytes. Cornea. 2002 Mar; 21(2):210-4. View in: PubMed. Effect of anti-inflammatory cytokines on the activation of lymphocytes by lacrimal gland acinar cells in an autologous mixed cell reaction. Adv Exp Med Biol. 2002; 506(Pt B):789-94. View in: PubMed. Autologous lacrimal-lymphoid mixed-cell reactions induce dacryoadenitis in rabbits. Exp Eye Res. 2000 Jul; 71(1):23-31. View in: PubMed. Lacrimal gland epithelial cells stimulate proliferation in autologous lymphocyte preparations. Exp Eye Res. 2000 Jul; 71(1):11-22. View in: PubMed. Sjögrens autoimmunity: how perturbation of recognition in endomembrane traffic may provoke pathological recognition at the cell surface. J Mol Recognit. 1998; 11(1-6):40-8. ...
Zoological Research
Polymeric immunoglobulin receptors(pIgR) are key participants in the formation and secretion of secretory IgA(S-IgA), which is critical for the prevention of microbial infection and colonization in the respiratory system. Although increased respiratory colonization and infections are common in HIV/AIDS, little is known about the expression of pIgR in the airway mucosa of these patients. To address this, the expression levels of pIgR in the tracheal mucosa and lungs of SHIV/SIV-infected rhesus macaques were examined by real-time RTPCR and confocal microscopy. We found that the levels of both PIGR mRNA and pIgR immunoreactivity were lower in the tracheal mucosa of SHIV/SIV-infected rhesus macaques than that in non-infected rhesus macaques, and the difference in pIgR immunoreactivity was statistically significant. IL-17A, which enhances pIgR expression, was also changed in the same direction as that of pIgR. In contrast to changes in the tracheal mucosa, pIgR and IL-17A levels were higher in the ...
AMERICAN GROUND TRANSPORT
As measured by the structural economic criteria set forth in part I, automobile manufacturing is one of the least competitive industries in the American economy.48 Its structural concentration is unprecedented. One firm, General Motors, alone accounts for 50 percent of industry sales; 97 percent of domestic production is centered in three firms; four firms manufacture virtually all passenger cars produced and sold in this country.49 This degree of concentration is considerably greater than that generally considered inimical to competitive conduct and performance.50 In fact, the added feature of a single dominant firm (GM) increases the likelihood that the industry will behave and perform in a manner not unlike that of a cohesive monopoly.51. The extent of vertical integration in this industry is unparalleled. All four firms are integrated upstream into major components production and downstream into exclusive franchised distribution. All produce their own bodies and most of their stampings. All ...
Aerospace and Automotive
The transportation sector moves towards greater energy efficiency in all areas (i.e. from component production over to fuel consumption) in order to meet the latest performance and environmental targets. The success and progress fulfilling these goals is highly dependent on the availability of new engineering materials and manufacturing methods that enable significant weight savings associated with simultaneous improvements in component performance. Titanium alloys exhibit higher specific strength than other structural materials as well as excellent corrosion and creep resistance up to about 500 °C. These properties represent many performance advantages for the transportation industry. Despite these benefits and the relatively large resource reserves (titanium is the fourth most abundant metal in the earths crust), titanium alloys still come at high production costs that limit their industrial usage.. Titanium-based components are mainly produced via the classical process of ingot metallurgy ...
Immune components of the intestinal mucosae of ageing and protein deficient mice<...
TY - JOUR. T1 - Immune components of the intestinal mucosae of ageing and protein deficient mice. AU - Lim, T. S.. AU - Messiha, N.. AU - Watson, R. R.. PY - 1981/1/1. Y1 - 1981/1/1. N2 - The studies were designed to investigate the effect of ageing and low protein (4%) diet on antibody-dependent cell-mediated cytotoxicity (ADCC) and IgA concentration in the intestine. Both ADCC activity and IgA concentration were shown to reach mature levels at 17 weeks old. The effect of ageing was greater on ADCC activity which showed a drastic drop in activity at 75 weeks old than IgA which had only a slight decline in concentration at this age. The low protein diet has a greater suppressive effect on IgA concentration than on ADCC activity. As the duration of feeding low protein to the young mice increased, greater decline in IgA concentration in the intestine was observed. There was, however, no significant difference from the normal mice in ADCC activity and IgA concentration of aged and adult mice given ...
3.3.2. Properties of Immunoglobulins
3.3.2. Properties of Immunoglobulins. General. All immunoglobulins appear to be glycoproteins but the carbohydrate content ranges from 2-3% for IgG to 12-14% for IgM, IgD and IgE. The physicochemical proper-ties of the immunoglobulins are summarized in Table 3.2. Each class possesses a characteristic type of heavy chain. Thus IgG possesses g chains; IgM, m chains; IgA, achains, IgD, dchains and IgE, echains. Variation in heavy chain structure within a class gives rise to immunoglobulin subclasses. For example the human IgG pool consists of four subclasses reflecting four distinct types of g heavy chain. The physicochemical properties of the immunoglobulins vary between the different classes. Note that IgA occurs in a dimeric form {sigA) in association with a protein chain termed the secretory piece. The diversity of structure of the different classes suggests that they perform different functions, in addition to their primary function of antigen binding. In spite of this diver-sity all ...
IgA receptor elisa and antibody
Shop IgA receptor ELISA Kit, Recombinant Protein and IgA receptor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Why do B cells mutate their immunoglobulin receptors?
B cells have the unique ability to acquire large numbers of point mutations in the variable segment of rearranged immunoglobulin (Ig) genes during a germinal center reaction. It is broadly accepted that somatic hypermutation (SHM) and affinity maturation are required to generate memory B cells and t …
CiNii Articles - Kato Yasuko
An Examination of Procedure for Measuring Secretory IgA in Saliva and the Relationship Between Allergy and IgA Concentration in Students. (Kawasaki Medical Welfare Journal Vol.7,No.1,1997 Abstracts) (1998 ...
Altered secretory immunoglobulin a on skin surface after intensive exercise<...
TY - JOUR. T1 - Altered secretory immunoglobulin a on skin surface after intensive exercise. AU - Eda, Nobuhiko. AU - Shimizu, Kazuhiro. AU - Suzuki, Satomi. AU - Tanabe, Yoko. AU - Lee, Eunjae. AU - Akama, Takao. PY - 2013/9/1. Y1 - 2013/9/1. N2 - Eda, N, Shimizu, K, Suzuki, S, Tanabe, Y, Lee, E, and Akama, T. Altered secretory immunoglobulin A on skin surface after intensive exercise. J Strength Cond Res 27(9): 2581-2587, 2013-The aim of this study was to determine the effects of high-intensity endurance exercise on skin immunity by estimating secretory immunoglobulin A (SIgA) and staphylococci on skin surface. Seven healthy adult men (age, 22.3 ± 2.0 years) performed bicycle exercise at 75% HRmax for 60 minutes from 2030 to 2130 hours. Secretory immunoglobulin A was obtained from 1 ml extraction liquids stirred with the microtube homogenizer in the open end of a polypropylene tube for 60 seconds. Secretory immunoglobulin A concentrations were measured using enzyme-linked immunosorbent assay. ...
VPS29 - Vacuolar protein sorting-associated protein 29 - Homo sapiens (Human) - VPS29 gene & protein
Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (Probable
Embryonic Mesenchymal Multipotent Cell Differentiation on Electrospun Biodegradable Poly(ester amide) Scaffolds for Model...
Vascular differentiation of stem cells and matrix component production on electrospun tubular scaffolds is desirable to engineer blood vessels. The mouse e
Further reading - Immune Response - Barnard Health Care
Fazel S, Wiersma EJ and Shulman MJ (1997) Interplay of J chain and disulfide bonding in assembly of polymeric IgM. International Immunology 9: 1149-1158.. Hendrickson BA, Conner DA, Ladd DJ et al (1995) Altered hepatic transport of immunoglobulin A in mice lacking the J chain, journal of Experimental Medicine 182: 1905-1911.. Inman FP and Mestecky J (1974) The J chain of polymeric immunoglobulins. Contemporary Topics in Molecular Immunology 3: 111-141.. Koshland ME (1985) The coming of age of the immunoglobulin J chain. Annual Revieiv of Immunology 3: 425-453.. Mestecky J and McGhee JR (1987) Immunoglobulin A (IgA): molecular and cellular interactions involved in IgA biosynthesis and immune response. Advances in Immunology 40: 153-245.. Takahashi T, Iwase T, Takenouchi N et al (1996) The Joining (J) chain is present in invertebrates that do not express immunoglobulins. Proceedings of the National Academy of Sciences of the USA 93: 1886-1891.. ...
Role of membrane receptors in the induction of an in vitro secondary a by S Cammisuli, C Henry et al.
Cammisuli, S; Henry, C; and Wofsy, L, Role of membrane receptors in the induction of an in vitro secondary anti-hapten response. I. Differentiation of b memory cells to plasma cells is independent of antigen- -immunoglobulin receptor interaction. (1978). Subject Strain Bibliography 1978. 3488 ...
INT162745 - wiki-pain
These aberrant forms of HLA-B27 can be recognized by immunomodulatory killer cell immunoglobulin receptors, such as KIR3DL1 and KIR3DL2, and the immunoglobulin-like transcript ILT-4 ...
Table of Contents | Infection and Immunity
Mucosal surfaces like those present in the lung, gut, and mouth interface with distinct external environments. These mucosal gateways are not only portals of entry for potential pathogens but also homes to microbial communities that impact host health. Secretory immunoglobulin A (SIgA) is the single most abundant acquired immune component secreted onto mucosal surfaces and, via the process of immune exclusion, shapes the architecture of... ...
SIGA Technologies Reports Financial Results for the Fourth Quarter and Year Ended 2012
NEW YORK, March 6, 2013-- SIGA Technologies, Inc., a company specializing in the development of pharmaceutical agents to fight pathogens capable of use as bioweapons, today reported its financial results for the quarter and year ended December 31, 2012.
Immunoglobulin M and Secretory Immunoglobulin A: Presence of a Common Polypeptide Chain Different from Light Chains | Semantic...
Unique polypeptide chains have been isolated from S-sulfonated light-chain fractions of human serum immunoglobulin M and colostral immunoglobulin A. Their electrophoretic mobilities, molecular weights, peptide maps, amino acid compositions, and antigenic determinants are very similar or perhaps identical but differ from those of light chains and secretory piece.
Sleeping Bunny Pattern #16 | Crochet Patterns
Gauge: 6 sc make 1 inch.. BODY-Starting at neck, ch 30. Join ends. 1st rnd: 2 sc in first ch, 2 in second, 12 sc, 2 sc in next 3 ch, 11 sc, 2 sc in last ch. 2nd rnd: 2 sc in 1 sc, 2 sc in 1 sc, 14 sc, 2 sc in 1 sc, 1 sc, 2 sc in 1 sc, 14 sc. 3rd: 2 sc in 1 sc, 16 sc, 2 sc in 1 sc, 2 sc, 2 sc in 1 sc, 15 sc, 2 sc in 1 sc. 4th: 1 sc, 2 sc in 1 sc, 16 sc, 2 sc in 1 sc, 4 sc, 2 sc in 1 sc, 15 sc, 2 sc in 1 sc, 1 sc. 5th: 2 sc, 2 sc in 1 sc, 15 sc, 2 sc in 1 sc, 6 sc, 2 sc in 1 sc, 16 sc, 2 sc in 1 sc, 1 sc. 6th: 3 sc, 2 sc in 1 sc, 15 sc, 2 sc in 1 sc, 15 sc, 7 sc, 2 sc in 1 sc, 2 sc in 1 sc, 4 sc. 7th: 3 sc, 2 sc in 1 sc, 14 sc, 2 sc in 1 sc, 9 sc, 2 sc in 1 sc, 14 sc, 2 sc in 1 sc, 6 sc. 8th: 2 sc, 2 sc in 1 sc, 17 sc, 2 sc in 1 sc, 9 sc, 2 sc in 1 sc, 18 sc, 2 sc in 1 sc, 5 sc. Sc till piece measures 3½ inches. Divide in quarters, dec. 1 each quarter. Repeat. Inc. in same place for 7 rnds. Divide in half to form legs. Starting from crotch; 1st rnd. sc. 2nd rnd: 11 hdc, 6 sc, 2 sc inc. 1 sc, 6 ...
2006 Honda FSC600A AC FRONT BRAKE MASTER CYLINDER (FSC600A) | Competition Accessories
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β2-Microglobulin Production and Secretion by Lymphocytes in Culture | The Journal of Immunology
A β2-microglobulin with molecular weight of 12,600 daltons was initially isolated from the urine and serum of patients with renal tubular insufficiency (1). Smithies and Poulik (2) determined the partial amino acid sequence of this globulin and found that 24 of the first 46 amino acid residues were homologous with the amino acid sequence of parts of the heavy chain of the G-1 myeloma protein. This suggested that a structural relationship existed between the β2-microglobulin and the constant region of human IgG immunoglobulins. In spite of this structural relatedness, specific antisera against all known immunoglobulin chains (including secretory piece and J-chain) did not react with this protein. Subsequently, Peterson et al. (3) established the complete sequence of this protein and found that 28 amino acid residues of the β2-microglobulin were homologous with the CH3 domain of the myeloma protein. An homologous protein was also isolated recently from the dog (4), and its partial amino acid ...
Antievolution.org - Antievolution.org Discussion Board -Topic::A Separate Thread for Gary Gaulin
When a B cell recognizes an antigen, it is stimulated to divide (or proliferate). During proliferation, the B cell receptor locus undergoes an extremely high rate of somatic mutation that is at least 10^5 - 10^6 fold greater than the normal rate of mutation across the genome.[2] Variation is mainly in the form of single base substitutions, with insertions and deletions being less common. These mutations occur mostly at hotspots in the DNA, known as hypervariable regions. These regions correspond to the complementarity determining regions; the sites involved in antigen recognition on the immunoglobulin.[4] The exact nature of this targeting is poorly understood, although is thought to be controlled by a balance of error-prone and high fidelity repair.[5] This directed hypermutation allows for the selection of B cells that express immunoglobulin receptors possessing an enhanced ability to recognize and bind a specific foreign antigen.[1 ...
Transglutaminase is essential for IgA nephropathy development acting through IgA receptors • Research - Institut Pasteur
IgA nephropathy (IgAN) is a common cause of renal failure worldwide. Treatment is limited because of a complex pathogenesis, including unknown factors
FUKUDA Mitsunori
Synaptotagmin-like proteins control the formation of a single apical membrane domain in epithelial cells..[Nature Cell Biol.,14(8),(2012),838-849]Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. E., Bryant, D. M., Vergarajauregui, S., Yasuda, T., Bañón-Rodríguez, I., Bernascone, I., Datta, A., Spivak, N., Young, K., Slim, C. L., Brakeman, P. R., Fukuda, M., Mostov, K. E. and Martín-Belmonte, F ...
Search of: Graves disease - List Results - ClinicalTrials.gov
Microbiome modification (beneficial modification of the gut microbioma relates to a reduction at the end of treatment of at least 5% of the Firmicutes:Bacteroides ratio and 30% of the anti-TSHr antibody titer and of total IgG and IgA concentrations ...
Start - Arctic Paper
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