Introduction: Soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) has been reported to be a novel biomarker for early diagnosis of acute coronary syndromes (ACS); however, it is unclear when serum sLOX-1 levels begin to increase before the onset of ACS. Lectin-like oxidized low-density lipoprotein receptor-1 that is expressed predominantly in lipid-laden area underneath a fibromuscular cap is released in part as sLOX-1 by proteolytic cleavage.. Hypothesis: We reasoned that plaque disruption, which exposed the lipid-burden to circulating blood, would play a key role of increasing the serum sLOX-1 levels.. Methods: We evaluated time-dependent changes in sLOX-1 levels during elective percutaneous coronary interventions (PCI) in the consecutive 43 patients with coronary heart disease (CHD). Intravascular ultrasound was used to confirm the occurrence of procedural plaque disruption during PCI. Serum sLOX-1 levels were measured immediately before PCI (baseline), 6 hours, 12 hours, ...
Background: Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) appears to play an important role in the atherosclerotic plaque vulnerability and rupture. LOX-1 is cleaved and released as soluble LOX-1 (sLOX-1), and elevated sLOX-1 level may be indicative of plaque instability. sLOX-1 level was shown to be a sensitive and specific biomarker for diagnosing acute coronary syndrome (ACS). Recently, sLOX-1 level was shown to be a reliable prognostic biomarker after ACS. It is unclear how the plasma sLOX-1 level at the acute phase is related to the prognosis in patients with ST-elevation acute myocardial infarction (STEMI).. Methods and Results: We examined the relation between sLOX-1 level at admission and prognosis prospectively in the consecutive 153 STEMI patients admitted within 24 hours after the onset. All patients underwent emergent coronary angiography and 144 were treated with emergent percutaneous coronary intervention. The patients were divided into 2 groups by the median ...
This study reports the molecular mechanisms responsible for the previously observed atherogenic properties of the adaptor protein p66Shc. In particular, it provides mechanistic evidence on how p66Shc mediates the deleterious effects of oxLDL uptake in the endothelium, eventually leading to vascular dysfunction and plaque formation. In primary HAECs, oxLDL induces phosphorylation of p66Shc at the Ser36 residue through activation of PKCβ2 and JNK, respectively. Further, we demonstrated that serial activation of PKCβ2 and JNK, eventually leading to p66Shc phosphorylation, is the main mechanism responsible for increased O2−· generation following exposure of HAECs to oxLDL.. p66Shc is the only isoform of the Shc adaptor protein family that acts as a redox enzyme. p66Shc appears to be equally involved in mediating acute and chronic oxidative stress, and it represents an important final common molecular pathway in aging and chronic cardiovascular disease.26 The latter effect of p66Shc may be of ...
The 3rd International Symposium on Healthy Aging (ISHA 2008), Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 1-2 March 2008 ...
Correlation between plasma concentration of soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) and brachial-ankle pulse wave velocity (baP
Aims The lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), encoded by the OLR1 gene, has been implicated in the pathogenesis of atherosclerosis. We therefore evaluated the genotyping of OLR1 gene in a sample of 55 patients with Metabolic Syndrome, a clinical condition characterized by a high cardiovascular risk.. Methods and Patients The genotyping of the LOX-1 was performed by polymerase chain reaction (PCR) analysis of the IVS4-14 A,G OLR1 polymorphism embedded within the OLR1 Linkage Disequilibrium block. Patients were assessed for routine serum parameters, microalbuminuria, insulin resistance (HOMA) and oxidative stress (thiobarbituric acid reactive substances, TBARs and thioredoxin).. Results The allele or genotype distribution of the OLR1 IVS4-14 A,G was not statistically different between MS and controls subjects. A positive association was found between IVS4-14 GG genotype, microalbuminuria and fasting glycaemia as well as a higher frequency of type 2 diabetes, elevated ...
References. 1. Gotsman I, Sharpe AH, Lichtman AH. T-cell costimulation and coinhibition in atherosclerosis. Circ Res 2008; 103: 1220-1231, doi: 10.1161/CIRCRESAHA.108.182428. [ Links ] 2. Ross R. Atherosclerosis - an inflammatory disease. N Engl J Med 1999; 340: 115-126, doi: 10.1056/NEJM199901143400207. [ Links ] 3. Tousoulis D, Davies G, Stefanadis C, Toutouzas P, Ambrose JA. Inflammatory and thrombotic mechanisms in coronary atherosclerosis. Heart 2003; 89: 993-997, doi: 10.1136/heart.89.9.993. [ Links ] 4. Liuzzo G. Atherosclerosis: an inflammatory disease. Rays 2001; 26: 221-230. [ Links ] 5. Hayashida K, Kume N, Minami M, Kita T. Lectin-like oxidized LDL receptor-1 (LOX-1) supports adhesion of mononuclear leukocytes and a monocyte-like cell line THP-1 cells under static and flow conditions. FEBS Lett 2002; 511: 133-138, doi: 10.1016/S0014-5793(01)03297-5. [ Links ] 6. Wang X, Ria M, Kelmenson PM, Eriksson P, Higgins DC, Samnegard A, et al. Positional identification of TNFSF4, encoding OX40 ...
Complete information for OLR1 gene (Protein Coding), Oxidized Low Density Lipoprotein Receptor 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for OLR1 gene (Protein Coding), Oxidized Low Density Lipoprotein Receptor 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Most importantly, L5 was significantly elevated in STEMI individuals when compared with otherwise healthy control subjects. These changes in the fractional composition of LDL, often called bad cholesterol because of its role in atherogenesis, may add thrombophilic properties. Platelets that were exposed to relevant dosages of purified L5 exhibited improved adenosine 5-diphosphate-stimulated aggregation medically, P-selectin manifestation, and GP IIb/IIIa activation with signaling through platelet-activating element receptor and lectin-like oxidized LDL receptor-1. Endothelium subjected to L5 inside a also manner expressed cells element and P-selectin that also backed platelet activation and aggregation. As shown previously, L5 also mediated endothelial apoptosis by reducing manifestation from the fibroblast development element-2 (FGF2) promoter via an epigenetic system (CpG methylation). Injecting L5 into mice corroborated these ex vivo results. Will raised L5 result in a domino impact thats ...
Mouse LOX-1 / OLR1 protein (1564-LX) is manufactured by R&D Systems, over 95% purity. Reproducible results in binding activity assays. Learn More...
Shop L-type lectin-like domain-containing protein ELISA Kit, Recombinant Protein and L-type lectin-like domain-containing protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
AIMS/INTRODUCTION: The purpose of the present study was to investigate the possible effect of oral magnesium sulfate (MgSO4 ) in the reduction of atherosclerosis plaques through inhibition of lectin-like low-density lipoprotein receptor-1 (LOX-1) gene expression in diabetic vessels. MATERIALS AND METHODS: A total of 50 rats were divided into five groups, including non-diabetic control, Mg-treated non-diabetic control, chronic diabetic, Mg-treated chronic diabetic and insulin-treated chronic diabetic. The induction of diabetes was carried out by streptozotocin. The Mg-treated chronic diabetic and Mg-treated non-diabetic control groups were treated with 10 g/L of MgSO4 added to their drinking water. The insulin-treated chronic diabetic group received 2.5 U/kg of insulin twice per day. The fasting blood glucose level and bodyweight were determined weekly. Blood pressure measurement and the intraperitoneal glucose tolerance test were carried out after 16 weeks, and the plasma levels of Mg, lipid ...
Perform reliable PCR with Bio-Rads Olr588 primer pair, for Rat. Designed for EvaGreen-based detection with digital PCR (ddPCR).
Perform reliable PCR with Bio-Rads Olr587 primer pair, for Rat. Designed for EvaGreen-based detection with digital PCR (ddPCR).
Generally, age-related testicular changes are associated with an increase of germ cell degeneration, decline of spermatogenesis and androgen decline resulting in a gradual decrease of sperm count. Unfortunately, an association of these findings to vascular atherosclerotic alterations has never been investigated systematically, although arterial lesions in testicular biopsies of azoospermic men have been described already 30 years ago.
Since the discovery of the lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1) by Sawamura and colleagues in 1997, this multi-ligand receptor has been implicated in atherosclerosis and diabetes. Oxidised LDL binding and trafficking via LOX-1 cause the activation of downstream signal transduction that cause pro-athrogenic changes such as endothelial dysfunction, apoptosis and foam cell formation. However, the molecular mechanisms have not be been fully explained. In this study, tetracycline- inducible cell lines expressing LOX-1 wild-type and trafficking-defective LOX-1-D5A were developed. The findings show different trafficking properties between LOX-1-WT and LOX-1-D5A in response to oxidised LDL. Due to these differences, LOX-1-WT and LOX-1-D5A in response to oxidised LDL exhibited differential downstream signal transduction. Moreover, 24 hour stimulation of oxidised LDL via LOX-1-WT caused decreased endothelial cell permeability; however, the underlying mechanism is not clear. The ...
... - useful links to statutes, OLR reports and websites concerning smoking, smoking in the workplace, public health issues and selling tobacco
Previously we reported that the P2Y2 receptor (P2Y2R) is one of the predominant purinergic receptors expressed in human coronary artery endothelial cells (HCAEC), and that P2Y2R activation by ATP or UTP induces dramatic up-regulation of tissue factor (TF), key initiator of the coagulation cascade. However, the molecular mechanism of this P2Y2R-TF axis remains unclear. Here we report a role of a newly identified AP-1 consensus sequence along with its new binding components in P2Y2R regulation of TF transcription. We identified with bioinformatics tools that a novel AP-1 site at -1363 bp of human TF promoter region is highly conserved across multiple species. P2Y2R activation increased TF promoter activity and mRNA expression in HCAEC. Truncation, deletion, and mutation of this new distal AP-1 site all significantly supressed TF promoter activity in response to P2Y2R activation. EMSA and ChIP assays further confirmed that upon P2Y2R activation, c-Jun, ATF-2 and Fra-1, but not the typical c-Fos, ...
Engineering of non-toxic nanoparticles for tissue-specific targeting has gained widespread attention this decade. Liposomes were one of the earliest classes of engineered nanoparticles to be utilized for the purpose of delivering drugs within the human body. While many have successfully designed and tested such
integral component of membrane, olfactory receptor activity, G-protein coupled receptor signaling pathway, sensory perception of smell
Adamkiewicz, T.V., McSherry, C., Bach, F.H. et al. Natural killer lectin-like receptors have divergent carboxyl-termini, distinct from C-type lectins. Immunogenetics 39, 218 (1994). https://doi.org/10.1007/BF00241264. Download ...
Hong Yu Wang, Chao Quan, Chunxiu Hu, Bingxian Xie, Yinan Du, Liang Chen, Wei Yang, Liu Yang, Qiaoli Chen, Bin Shen, Bian Hu, Zhihong Zheng, Haibo Zhu, Xingxu Huang, Guowang Xu, Shuai Chen ...
How is Quasi-Cycle Low-Density Parity Check abbreviated? QC-LDPC stands for Quasi-Cycle Low-Density Parity Check. QC-LDPC is defined as Quasi-Cycle Low-Density Parity Check very rarely.
METHODS AND RESULTS: Endothelial-specific LOX-1 transgenic mice were generated using the Tie2 promoter (LOX-1TG). Oxidized low-density lipoprotein uptake was enhanced in cultured endothelial cells, but not in macrophages of LOX-1TG mice. Six-week-old male LOX-1TG and wild-type (WT) mice were fed a high-cholesterol diet (HCD) for 30 weeks. Increased reactive oxygen species production, impaired endothelial nitric oxide synthase activity and endothelial dysfunction were observed in LOX-1TG mice as compared with WT littermates. LOX-1 overexpression led to p38 phosphorylation, increased nuclear factor κB activity and subsequent up-regulation of vascular cell adhesion molecule-1, thereby favouring macrophage accumulation and aortic fatty streaks. Consistently, HCD-fed double-mutant LOX-1TG/ApoE(-/-) displayed oxidative stress and vascular inflammation with higher aortic plaques than ApoE(-/-) controls. Finally, bone marrow transplantation experiments showed that endothelial LOX-1 was sufficient for ...
The lymphatic endothelial receptor LYVE-1 has been implicated in both uptake of hyaluronan (HA) from tissue matrix and in facilitating transit of leukocytes and tumor cells through lymphatic vessels based largely onin vitrostudies with recombinant receptor in transfected fibroblasts. Curiously, however, LYVE-1 in lymphatic endothelium displays little if any binding to HAin vitro, and this has led to the conclusion that the native receptor is functionally silenced, a feature that is difficult to reconcile with its proposedin vivofunctions. Nonetheless, as we reported recently, LYVE-1 can function as a receptor for HA-encapsulated Group A streptococci and mediate lymphatic dissemination in mice. Here we resolve these paradoxical findings and show that the capacity of LYVE-1 to bind HA is strictly dependent on avidity, demanding appropriate receptor self-association and/or HA multimerization. In particular, we demonstrate the prerequisite of a critical LYVE-1 threshold density and show that HA binding may
Our present results establish for the first time that MTI-induced apoptosis and 15-LOX-1 expression are linked mechanistically in colorectal cancer cells. The demethylating agents 5-Azadc, 5-azacytidine, and zebularine can induce 15-LOX-1 expression, indicating that methylation may play a role in 15-LOX-1 regulation. 5-Azadc induced 15-LOX-1 expression in a dose- and time-dependent manner and induced growth arrest and apoptosis in Caco-2 colon cancer cells. Inhibiting 15-LOX-1 with caffeic acid (at the 2.2 μmol/L concentration shown to be specific for 15-LOX-1 inhibition) or siRNA attenuated 5-Azadc-induced growth inhibition and apoptosis, indicating a direct relationship between 15-LOX-1 and these biological effects. These findings identify a molecular mechanism by which MTIs induce apoptosis and other cellular effects and further elucidate the role of 15-LOX-1 in human colorectal carcinogenesis. Our findings are consistent with results of other studies, which showed a 15-LOX-1-apoptosis link ...
Chung-Sheng Shi, Guey-Yueh Shi, Hsi-Min Hsiao, Yuan-Chung Kao, Kuan-Lin Kuo, Chih-Yuan Ma, Cheng-Hsiang Kuo, Bi-Ing Chang, Chuan-Fa Chang, Chun-Hung Lin, Chi-Huey Wong, Hua-Lin Wu
Bacteria produce a diverse array of antagonistic compounds to restrict growth of microbial rivals. Contributing to this warfare are bacteriocins: secreted antibacterial peptides, proteins and multi-protein complexes. These compounds typically eliminate competitors closely related to the producer. Lectin-like bacteriocins (LlpAs) constitute a distinct class of such proteins, produced by Pseudomonas as well as some other proteobacterial genera. LlpAs share a common architecture consisting of two B-lectin domains, followed by a short carboxy-terminal extension. Two surface-exposed moieties on susceptible Pseudomonas cells are targeted by the respective lectin modules. The carboxy-terminal domain binds D-rhamnose residues present in the lipopolysaccharide layer, whereas the amino-terminal domain interacts with a polymorphic external loop of the outer-membrane protein insertase BamA, hence determining selectivity. The absence of a toxin-immunity module as found in modular bacteriocins and other polymorphic
Looking for online definition of low-density lipoprotein receptor class A domain-containing protein 1 in the Medical Dictionary? low-density lipoprotein receptor class A domain-containing protein 1 explanation free. What is low-density lipoprotein receptor class A domain-containing protein 1? Meaning of low-density lipoprotein receptor class A domain-containing protein 1 medical term. What does low-density lipoprotein receptor class A domain-containing protein 1 mean?
Mediators of Inflammation is a peer-reviewed, Open Access journal that publishes original research and review articles on all types of inflammatory mediators, including cytokines, histamine, bradykinin, prostaglandins, leukotrienes, PAF, biological response modifiers and the family of cell adhesion-promoting molecules.
The molecular mechanisms of atherosclerosis development are complex and still poorly understood. Recent studies revealed that TLR/IL-1R signaling is involved in the development of atherosclerotic plaques. In this study, we investigated the role of IRAK4 kinase activity in the development of atherosclerosis. NF-κB activation and NF-κB-dependent proinflammatory gene expression were abrogated in IRAK4KI macrophage upon addition of putative atherogenic ligands acLDL and NO2-LDL. IRAK4 protein, especially its kinase activity, is required for the aortic plaque formation as well as proinflammatory cytokine production important for the development of atherosclerosis. Although the total cholesterol and plasma lipoprotein distribution are comparable, IRAK4KI/ApoE−/− mice showed dramatic reduction in aortic sinus lesion size compared with that in ApoE−/− mice, indicating that the critical role of IRAK4 kinase activity in the development of atherosclerosis through the regulation of ...
Gokce G, Ozsarlak-Sozer G, Oran I, Oktay G, Ozkal S, Kerry Z. Taurine suppresses oxidative stress-potentiated expression of lectin-like oxidized low-density lipoprotein receptor and restenosis in balloon-injured rabbit iliac artery. Clin Exp Pharmacol Physiol. 2011;38(12):811-8 ...
Exercse 1 DETERINTION OF OLR SS FRO FREEZING POINT DEPRESSION Collgatve propertes The propertes we now consder are the lowerng of vapour pressure, the elevaton of bolng pont, the depresson of freezng pont,
The deciduous tree Terminalia bellirica found in Southeast Asia is extensively used in traditional Indian Ayurvedic medicine for the treatment of hypertension, rheumatism, and diabetes. The anti-atherogenic effect of Terminalia bellirica fruit has not been fully elucidated. Here, we investigated the effect of Terminalia bellirica extract (TBE) on low-density lipoprotein (LDL) oxidation and inflammation in macrophages. TBE showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (EC50: 7.2 ± 1.2 μg/mL) and 15-lipoxygenase inhibitory activity. TBE also significantly inhibited free radical-induced LDL oxidation compared to the solvent control in vitro. In THP-1 macrophages, TBE treatment resulted in significant decreases of the mRNA expression of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and lectin-like oxidized LDL receptor-1 (LOX-1). TBE also reduced matrix metalloproteinase (MMP)-9 secretion and intracellular reactive oxygen species (ROS) production in THP-1
Antibody Database - Conjugates for the antigen Killer cell lectin-like receptor subfamily A, member 3 / KLRA3 in the Antibody Database
Reagents for the antigen Killer cell lectin-like receptor subfamily C, member 2 / KLRC2 stained with 7C in the Antibody Database
Low-density lipoprotein receptor (LDL-R) is a cell surface receptor protein expressed in a variety of solid cancers including lung, colon, breast, brain and liver, therefore opens up opportunities to deliver lysosome sensitive anti-cancer agents, especially synthetic nucleic acid-based therapeutic mole- cules. In this study, we focused on developing novel nucleic acid molecules specific to LDL-R. For this purpose, we performed in vitro selection procedure via SELEX methodologies using mammalian cell- expressed human recombinant LDL-R protein as a target. After ten rounds of selections, we identified a novel DNA oligonucleotide aptamer, RNV-L7, that can bind specifically to LDL-R protein with high affinity and specificity (Kd = 19.6 nM). Furthermore, flow cytometry and fluorescence imaging assays demonstrated efficient binding to LDL-R over-expressed human cancer cells including Huh-7 liver can- cer cells and MDA-MB-231 breast cancer cells with a binding affinity of ∼200 nM. Furthermore, we ...
BACKGROUND: In addition to being a risk marker for cardiovascular disease, much recent data suggest that C-reactive protein (CRP) promotes atherogenesis. Decreased endothelial NO and prostacyclin (PGI2) contribute to a proatherogenic and prothrombotic state. We have shown that CRP decreases endothelial NO synthase expression and bioactivity in human aortic endothelial cells (HAECs). PGI2 is a potent vasodilator and inhibitor of platelet aggregation. Hence, the aim of this study was to examine the effect of CRP on PGI2 release from HAECs and human coronary artery endothelial cells (HCAECs ...
CD93是一個高度醣基化的穿膜蛋白,普遍地表現在內皮細胞、嗜中性白血球、幹細胞裡。因為CD93與thrombomodulin 的結構相似;且同屬於C-type lectin-like domain superfamily。目前已知thrombomodulin 在細胞與細胞之間貼附扮演重要角色,可以透過其lectin-like domain ...
Dear Sir, you should be using the adiabatic formulae? Your derived pressure lapse rate is for constant temperature. The principle is sound, however the adiabatic formula cannot have T = 255 at the centre of mass of the column of atmosphere. Although, I understand you have used the average temperature in the isometric formula. I am convinced that the +33C is due to gravitational redistribution of kinetic energies of molecules. I am still working on the problem in my own time. Observations from radiosondes actually give a better fit to pressure with height using the isometric equation you describe, than with the adiabatic formula, this is a bit confusing for me and I am still working on it. The radiosonde data I have looked at shows the average temperature (weighted by mass of course) of the atmospheric column is equal to the OLR measured by satellite - within a couple of percent. I got the OLR data from KNMI. I am looking for a more rigorous demonstration - i.e. resisting the urge to use the ...
1996 (English)In: Chemické listy (Print), ISSN 0009-2770, E-ISSN 1213-7103, Vol. 90, no 9, 698-699 p.Article in journal (Refereed) Published ...
Elder GA, Cho JY, English DF, Franciosi S, Schmeidler J, Sosa MA, Gasperi RD, Fisher EA, Mathews PM, Haroutunian V, Buxbaum JD. Elevated plasma cholesterol does not affect brain Abeta in mice lacking the low-density lipoprotein receptor ...
Kim J, Castellano JM, Jiang H, Basak JM, Parsadanian M, Pham V, Mason SM, Paul SM, Holtzman DM. Overexpression of low-density lipoprotein receptor in the brain markedly inhibits amyloid deposition and increases extracellular A beta clearance ...
B-1 cells and IgM NAbs. In mice, B-1a cells are more properly regarded as an arc of innate immunity. They respond to T cell-independent antigens by secretion of IgM and IgA NAbs, whose repertoire results from natural selection. Importantly, OSEs such as those found on oxLDL and apoptotic cells are a major target of NAbs in both mice and humans (reviewed in ref. 7). Considerable evidence supports an atheroprotective role for IgM specific for OSEs in mice. Immunization of mice with heat-killed S. pneumoniae, which shares molecular identity with oxidized phospholipids of oxLDL, leads to marked increases in IgM specific for oxLDL, which blocks oxLDL uptake by macrophages and inhibits atherosclerosis (79). T-bet deficiency and CD74 deficiency, both associated with reduced atherosclerosis, result in marked increases in IgM NAbs specific for oxLDL (33, 80). Ldlr-/- mice deficient in the ability to secrete IgM (sIgM-/- mice) have dramatically increased atherosclerosis (81). Splenectomy was shown to ...
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