Aim: Glycosylation of serum proteins is affected with prolonged heavy drinking, and carbohydrate deficient transferrin (CDT) is well established and highly specific biomarker of sustained alcohol consumption. However, total amount of sialic acid is not the only glycoepitope that may be altered as a result of the disease. This work is focused on glycan structures altered in salivary glycoproteins of alcoholics, indicating the most efficient carriers of such marker glycoepitopes. Methods: Salivary glycoproteins of 31 alcohol-dependent patients and 21 healthy controls were studied by means of lectin ELISA and lectin blotting with the lectins specific for core and antennary fucose, α2,3-bound sialic acid as well as T and Tn antigens in O-glycans. Results: In direct lectin ELISA, core fucosylation, α2,3 sialylation and expression of T-antigen were significantly lowered in the saliva of alcohol-dependent patients. In lectin blotting ten glycoprotein bands were analyzed. The profile of ...
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Salp15 is a tick saliva protein that inhibits CD4(+) T cell differentiation through its interaction with CD4. The protein inhibits early signaling events during T cell activation and IL-2 production. Because murine Experimental Autoimmune Encephalomyelitis development is mediated by central nervous system-infiltrating CD4(+) T cells that are specific for myelin-associated proteins, we sought to determine whether the treatment of mice with Salp15 during EAE induction would prevent the generation of proinflammatory T cell responses and the development of the disease. Surprisingly, Salp15-treated mice developed more severe EAE than control animals. The treatment of EAE-induced mice with the tick saliva protein did not result in increased infiltration of T cells to the central nervous system, indicating that Salp15 had not affected the permeability of the blood-brain barrier. Salp15 treatment did not affect the development of antibody responses against the eliciting peptide or the presence of IFNγ ...
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The overall goal of our study was to compare the proteins found in the saliva proteomes of three mammals: human, mouse and rat. Our first objective was to compare two human proteomes with very different analysis depths. The 89 shared proteins in this comparison apparently represent a core of highly-expressed human salivary proteins. Of the proteins unique to each proteome, one-half to 2/3 lack signal peptides and probably are contaminants instead of less highly-represented salivary proteins. We recently published the first rodent saliva proteomes with saliva collected from the genome mouse (C57BL/6) and the genome rat (BN/SsNHsd/Mcwi). Our second objective was to compare the proteins in the human proteome with those we identified in the genome mouse and rat to determine those common to all three mammals, as well as the specialized rodent subset. We also identified proteins unique to each of the three mammals, because differences in the secreted protein constitutions can provide clues to differences in
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The present invention relates to the identification of novel oral cancer and periodontal disease biomarkers. Further, the present invention provides novel methods of diagnosing and for providing a pro
Ticks are obligate blood sucking parasites which transmit a wide range of pathogens worldwide including protozoa, bacteria and viruses. Additionally, tick feeding alone may result in anemia, dermatosis and toxin-induced ...
Mosquitoes are able to adapt to feed on blood by the salivary glands which created a protein that works against the haemostasis process. This study aims to investigate the salivary glands proteins expression of 50 adult female An. dirus A mosquitoes, a main vector of malaria in Thailand, each group with an age of 5 days which were artificial membrane fed on sugar, normal blood, blood infected with P. vivax, and blood infected with P. falciparum. Then mosquito salivary gland proteins were analyzed by SDS-PAGE on days 0, 1, 2, 3, and 4 after feeding. The findings revealed that the major salivary glands proteins had molecular weights of 62, 58, 43, 36, 33, 30, and 18 kDa. One protein band of approximately 13 kDa was found in normal blood and blood infected with P. vivax fed on day 0. A stronger protein band, 65 kDa, was expressed from the salivary glands of mosquitoes fed with P. vivax- or P. falciparum-infected blood on only day 0, but none on days 1 to 4. The study shows that salivary glands proteins
TY - JOUR. T1 - The crystal structure of nitrophorin 2. A trifunctional antihemostatic protein from the saliva of Rhodnius prolixus. AU - Andersen, John F.. AU - Montfort, William R.. PY - 2000/9/29. Y1 - 2000/9/29. N2 - Nitrophorin 2 (NP2) (also known as prolixin-S) is a salivary protein that transports nitric oxide, binds histamine, and acts as an anticoagulant during blood feeding by the insect Rhodnius prolixus. The 2.0-Å crystal structure of NP2 reveals an eight-stranded antiparallel β-barrel containing a ferric heme coordinated through His57, similar to the structures of NP1 and NP4. All four Rhodnius nitrophorins transport NO and sequester histamine through heme binding, but only NP2 acts as an anticoagulant. Here, we demonstrate that recombinant NP2, but not recombinant NP1 or NP4, is a potent anticoagulant; recombinant NP3 also displays minor activity. Comparison of the nitrophorin structures suggests that a surface region near the C terminus and the loops between β strands B-C and ...
Occurrence of gustducin-immunoreactive cells in von Ebners glands of guinea pigs. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Saliva of the blood feeding sand fly Lutzomyia longipalpis was previously shown to inhibit the alternative pathway (AP) of the complement system. Here, we have identified Lufaxin, a protein component in saliva, as the inhibitor of the AP. Lufaxin inhibited the deposition of C3b, Bb, Properdin, C5b and C9b on agarose-coated plates in a dose dependent manner. It also inhibited the activation of factor B in normal serum, but had no effect on the components of the membrane attack complex. Surface plasmon resonance (SPR) experiments demonstrated that Lufaxin stabilizes the C3b-B proconvertase complex when passed over a C3b surface in combination with factor B. Lufaxin was also shown to inhibit the activation of factor B by factor D in a reconstituted C3b-B, but did not inhibit the activation of C3 by reconstituted C3b-Bb. Proconvertase stabilization does not require the presence of divalent cations, but addition of Ni2+ increases the stability of complexes formed on SPR surfaces. Stabilization of the C3b-B
Histatins are antimicrobial and antifungal proteins, and have been found to play a role in wound-closure.[2][3] A significant source of histatins is found in the serous fluid secreted by Ebners glands, salivary glands at the back of the tongue, and produced by Acinus cells.[4] Here they offer some early defense against incoming microbes.[5] The three major histatins are 1, 3, and 5. Histatin 2 is a degradation product of histatin 1, and all other histatins are degradation products of histatin 3. Therefore there are only two genes, HTN1 and HTN3. Histatins antifungal properties has been seen with fungus such as Candida glabrata, Candida krusei, Saccharomyces cerevisiae, and Cryptococcus neoformans.[6] Histatins also precipitate tannins from solution - thus preventing alimentary adsorption.[7] ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Human salivary glands and the oral mucosa secrete a wide spectrum of antimicrobial agents which are believed to be essential for the protection and maintenance of a healthy oral environment. Histatins are believed to play a key role in controlling opportunistic fungal pathogens, such as Candida albicans, in the oral cavity. An increase in the immunocompromised population over the last few decades has established Candida spp. as clinically important oral fungal pathogens. Widespread use of the limited numbers of antifungal agents available to treat candidal infections has led to the rapid development of drug-resistant strains, which are the main cause for antifungal treatment failures. Histatins are naturally occurring antimicrobial peptides which have potent anticandidal activity at physiological concentrations, are non-toxic to mammalian cells, and do not induce resistance. Their mechanism of action is distinct from that of the azole-based antifungal drugs, and they are fungicidal against both ...
A gel-based proteomics approach was used to screen for proteins of differential abundance between the saliva of smokers and those who had never smoked. Subjecting precipitated proteins from whole human saliva of healthy non-smokers to two-dimensional electrophoresis (2-DE) generated typical profiles comprising more than 50 proteins. While 35 of the proteins were previously established by other researchers, an additional 22 proteins were detected in the 2-DE saliva protein profiles generated in the present study. When the 2-DE profiles were compared to those obtained from subjects considered to be heavy cigarette smokers, three saliva proteins, including interleukin-1 receptor antagonist, thioredoxin and lipocalin-1, showed significant enhanced expression. The distribution patterns of lipocalin-1 isoforms were also different between cigarette smokers and on-smokers. The three saliva proteins have good potential to be used as biomarkers for the adverse effects of smoking and the risk for inflammatory and
Molecularly imprinted polymers (MIPs) have long been studied for applications in biomolecule recognition and binding; compared with natural antibodies, they may offer advantages in cost and stability. We report on the development of MIPs that “self-report†concentrations of bound analytes via fluorescence changes in embedded quantum dots (QDots). Composite QDot/MIPs were prepared using phase inversion of poly(ethylene- co-vinyl alcohol) (EVAL) solutions with various ethylene mole ratios in the presence of salivary target molecules (e.g. amylase, lipase, and lysozyme). These major protein components of saliva have been implicated as possible biomarkers for pancreatic cancer. The optimum (highest imprinting effectiveness) ethylene mole ratios of the commercially available EVALs were found to be 32, 38, and 44 mol% for the imprinting of amylase, lipase, and lysozyme, respectively. QD fluorescence quenching was observed on binding of analytes to composite MIPs in a concentration-dependent ...
Davison, Glen, Allgrove, Judith and Gleeson, Michael (2009) Salivary antimicrobial peptides (LL-37 and alpha-defensins HNP1-3), antimicrobial and IgA responses to prolonged exercise. European Journal of Applied Physiology, 106 (2). pp. 277-284. ISSN 1439-6319 (doi:10.1007/s00421-009-1020-y) ...
|p|Peptide YY|sub|3-36|/sub| is a satiation hormone released postprandially into the bloodstream from L-endocrine cells in the gut epithelia. In the current report, we demonstrate PYY|sub|3-36|/sub| is also present in murine as well as in human saliva. In mice, salivary PYY|sub|3-36|/sub| derives from plasma and is also synthesized in the taste cells in taste buds of the tongue. Moreover, the cognate receptor Y2R is abundantly expressed in the basal layer of the progenitor cells of the tongue epithelia and von Ebners gland. The acute augmentation of salivary PYY|sub|3-36|/sub| induced stronger satiation as demonstrated in feeding behavioral studies. The effect is mediated through the activation of the specific Y2 receptor expressed in the lingual epithelial cells. In a long-term study involving diet-induced obese (DIO) mice, a sustained increase in PYY|sub|3-36|/sub| was achieved using viral vector-mediated gene delivery targeting salivary glands. The chronic increase in salivary PYY|sub|3-36|/sub|
Saliva may be the key to explaining the age and sex bias exhibited by influenza and other diseases, according to a new study. Published in ACS Journal of
Ticks are obligate blood sucking parasites which transmit a wide range of pathogens worldwide including protozoa, bacteria and viruses. Additionally, tick feeding alone may result in anemia, dermatosis and toxin-induced ...
Practical advice, tutorials, and peer-reviewed research on liquid chromatography (HPLC), gas chromatography (GC), mass spectrometry (MS) and related techniques.
Histatins are peptides exclusively present in the saliva of higher primates. They are secreted by the salivary glands and play an important role in protection of the mouth. Recently it was found that one of the histatins, histatin 1 (Hst1), improves cellsubstrate adhesion as well as cell-cell adhesion, suggesting a role in maintenance of the epithelial architecture and barrier function. The effect of Hst1 on cell-substrate adhesion is very pronounced. In vitro studies show that even on glass, a substrate on which adherent cells not readily attach, cells attach rapidly in the presence of Hst1. Preliminary results also show that Hst1 improves cell adhesion on titanium.. Click here for more information on this collaboration opportunity. ...
En CD4 oppregning metoden, α-test, er beskrevet som bruker hele spytt for å gi raske og nøyaktige CD4-tall. De α-test koster pennies og ...
Definition of salivary proline-rich proteins in the Definitions.net dictionary. Meaning of salivary proline-rich proteins. What does salivary proline-rich proteins mean? Information and translations of salivary proline-rich proteins in the most comprehensive dictionary definitions resource on the web.
A family of proline-rich proteins that constitute the majority of the protein component of SALIVA. Salivary proline-rich proteins occur as acidic, basic and glycosylated basic proteins. They perform a variety of functions such as adhering to the acquired ENAMEL PELLICLE, acting as lubricants and precipitating TANNINS ...
TY - JOUR. T1 - Role of binding site loops in controlling nitric oxide release. T2 - Structure and kinetics of mutant forms of nitrophorin 4. AU - Maes, Estelle M.. AU - Weichsel, Andrzej. AU - Andersen, John F.. AU - Shepley, Donald. AU - Montfort, William R.. PY - 2004/6/1. Y1 - 2004/6/1. N2 - Nitrophorins are ferric heme proteins that transport nitric oxide (NO) from blood-sucking insects to victims. NO binding is tighter at lower pH values, as found in the insect salivary gland, and weaker at the pH of the victims tissue, facilitating NO release and subsequent vasodilation. Previous structural analyses of nitrophorin 4 (NP4) from Rhodnius prolixus revealed a substantial NO-induced conformational change involving the A-B and G-H loops, which rearrange to desolvate the distal pocket and pack nonpolar residues against the heme-ligated NO. Previous kinetic analyses revealed a slow, biphasic, and pH-dependent NO release, which was proposed to be associated with loop movements. In this study, we ...
Objective: The composition of the salivary interface (pellicle) between dental restorations and oral mucosa may be critical to the biocompatibility of the restoration. The purpose of this study was to examine the molecular composition of the salivary pellicle on nickel-chromium alloy in vivo. Method and materials: The molecular components of nickel-chromium pellicle was examined with sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot analyses. Results: Only limited numbers of salivary proteins were found to participate in the formation of nickel-chromium pellicle in vivo. Salivary amylase and secretory immunoglobulin A were among the proteins identified in the pellicle. Conclusion: In vivo, nickel-chromium pellicle consists of selectively adsorbed salivary proteins. Because both salivary amylase and secretory immunoglobulin A are antimicrobial proteins, it is possible that they play a role in modulating the microbial flora on the nickel-chromium prosthesis ...
article{f429a20c-cc6f-41ca-9f07-404d5d006fd6, abstract = {Cysteine-rich secretory protein 3 (CRISP-3; also known as SGP28) was originally discovered in human neutrophilic granulocytes. We have recently developed a sensitive sandwich enzyme-linked immunosorbent assay for CRISP-3 and demonstrated the presence of CRISP-3 in exocrine secretions. To investigate the subcellular localization and mobilization of CRISP-3 in human neutrophils, we performed subcellular fractionation of resting and activated neutrophils on three-layer Percoll density gradients, release-studies of granule proteins in response to different secretagogues, and double-labeling immunogold electron microscopy. CRISP-3 was found to be localized in a subset of granules with overlapping characteristics of specific and gelatinase granules and mobilized accordingly, thus confirming the hypothesis that peroxidase-negative granules exist as a continuum from specific to gelatinase granules regarding protein content and mobilization. ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Iron atom in PDB 1koi: Crystal Structure of Nitrophorin 4 From Rhodnius Prolixus Complexed With Nitric Oxide At 1.08 A Resolution
7] "Molecular basis of salivary proline-rich protein and peptide synthesis: cell-free translations and processing of human and macaque statherin mRNAs and partial amino acid sequence of their signal peptides." Oppenheim F.G.et.al. 3476566 ...
This graph shows the total number of publications written about "Histatins" by people in this website by year, and whether "Histatins" was a major or minor topic of these publications ...
Fingerprint Dive into the research topics of Leech antihemostatic proteins share the T-knot scaffold, a disulfide-reinforced structural motif. Together they form a unique fingerprint. ...
A study published online in The FASEB Journal delves into the mystifying fact that wounds in your mouth heal faster and more efficiently than wounds elsewhere. Until now, it was understood that saliva played a part in the wound healing process, though the extent of its role was unknown. The study examined the effects of salivary peptide histatin-1 on angiogenesis (blood vessel formation), which is critical to the efficiency of wound healing. Researchers found that histatin-1 promotes angiogenesis, as well as cell adhesion and migration. (more…). Read More ...
The self-assembling properties of thermally-sensitive amphiphilic elastin-like multiblock recombinamers have been combined with the capacities of calcium phosphate binding of the SN(A)15 epitope inspired by the salivary protein statherin. In this regard, the interaction between calcium and phosphate ions was examined in the presence of two hybrid recombinamers. The first recombinamer comprised a simple amphiphilic diblock in which the SN(A)15 epitopes were combined, at the gene level, to the hydrophilic end. This recombinamer can self-assemble into nanoparticles that can control the transformation of amorphous calcium phosphate (ACP) into a fibre-like hydroxyapatite structure. In the other recombinamer, the SN(A)15 domains are distributed along the monomer chain, with the hydrophilic blocks being distributed amongst the hydrophobic ones. In this case, the resulting nanohybrid ACP/recombinamer organises into neuron-like structures. Thus, combining the amphiphilic elastin-like recombinamers to the ...
Human saliva contains a natural painkiller called opiorphin. Due to its powerful painkilling ability, scientists are trying to synthesize a new natural painkiller from opiorphin. It is great, because it doesnt have the same addicting attributes that morphine does and would work better with the body. In a study, 1 milligram of opiorphin gave the same results as 3 milligrams of morphine did. In another study, involving rats standing on pins, they needed 6 times more morphine than opiorphin to make them oblivious to the pain. Opiorphin works in the nerve cells of the spine. It stops the usual destruction of natural pain killing opiates in the spine called enkephalins. It is such a simple molecule, that scientists believe itll be possible to synthesize it and produce large quantities of it without needing to isolate it from saliva. Another approach is to find a drug that will make the body produce more opiorphin to manage pain.. (Source). ...
9. Feeling of warmth. Mosquito Facts. Mosquitoes are flying insects that tend to be more prevalent where there is standing water. They are more active early in the morning and early in the evening. Female mosquitoes lay their eggs in stagnant water. Only the female mosquito bites and feeds on human blood, as they need this blood in order to produce their eggs. Male mosquitoes feed on water and nectar. When a person is bitten, the mosquito injects its saliva into the skin which contains proteins that prevent the human blood from clotting. This allows the blood to be transferred to the mosquitos mouth. The typical localized itching, swelling, and/or redness of the skin that results from the bite is not directly due to the bite itself, but rather caused by the bodys immune response to the proteins in the mosquitos saliva. An "allergic reaction" to a mosquito bite is defined when there is a severe immune reaction against the salivary proteins of the mosquito, thus causing the more severe symptoms ...
Saliva is an important body fluid and can be used to monitor a patients health status. It contains multiple biomarkers, which gives it unique diagnostic potential. Different conditions, including some cancers, infectious diseases, and heart diseases, can be detected by testing your saliva.. Salivary diagnostics is not a new field in medicine. However, it has not been the bodily fluid of choice because the tested components appear in relatively small amounts when compared to blood serum. This makes routine diagnostics somewhat challenging. Some cutting-edge technology has recently been introduced that is changing this. A group of Indian scientists has been working on the oral fluid nanosensor test, which could non-invasively detect biomarkers for oral cancer. The nanosensor test is ultrasensitive and combines various techniques to detect salivary proteins and RNA biomarkers. Biochip technology is also being implemented in salivary diagnostics.. These point of care devices are miniature sensor ...
Our research into the molecular mechanisms of charge-dependent transport across biological membranes is directed toward two classes of proton pumps (P-type and V-type), a family of proton-coupled potassium transporters (TRK proteins), and a peculiar group of potassium channels which form as intramembrane homodimers (TOKs). All of these are studied in microorganisms, especially fungi, made accessible by full-genome sequences and by advanced electrophysiological techniques. A new, completely unexpected, direction for this research as been the investigation of interactions between specific membrane proteins and the rapidly growing catalogue of small proteins known as "host-defense peptides" or Ribosomally synthesized AntiMicrobial peptides ("RAMPs"). Some of these kill microorganisms by directly forming membrane pores, but more act by stealth, subverting the functions of surface proteins by reaction from the cell interior. One group of RAMPs, the salivary histatins, kills each of our three current ...
1) INSERM U289, Pitié-Salpêtrière, Paris, France; (2) CNRS UPR 640, Pitié-Salpêtrière, Paris, France; (3) INRIA Epidaure, Sophia-Antipolis, France; (4) Neuroradiology Dept., Pitié-Salpêtrière, Paris, France; (5) CIC, Pitié-Salpêtrière, Paris, ...
The purpose of this study is to better understand the genetic causes of cancer and the inherited tendency to develop cancer. To accomplish this, blood specimens and/or saliva samples and/or tumor and normal tissue blocks from patients and families of patients with cancer will be collected. Blood specimens will be frozen and stored for analysis at a later date. Tumor tissue and normal tissue will be stored for analysis at a later date. In order to perform this study, patients and members of their families will be asked to provide blood samples and/or saliva samples. Individuals will be asked to provide a history of cancer in their relatives at the time the blood sample is given. No relatives will be contacted before they have been asked by a family member if they wish to participate in this study. If they do wish to participate, the relatives should indicate this by returning the Family Member Consent for Contact Form After we receive this form, arrangements may be made for the family member to ...
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O. Zitka, S. Skalickova, S. Krizkova, M. Vlkova, V. Adam, R. Kizek Chromatographia (2013) 76:611-619 In this study, we optimized method for the isolation
WKP Saliva is a watery substance formed in the mouths of animals, secreted by the salivary glands. Human saliva comprises 99.5% mostly water, (...)
WKP Saliva is a watery substance formed in the mouths of animals, secreted by the salivary glands. Human saliva comprises 99.5% mostly water, (...)
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The collaboration of researchers of five universities reported on Tuesday that it has cataloged all 1,116 unique proteins found in human saliva glands, approximately 20 percent of which are also found in blood, said Fred Hagan, a researcher at the University of Rochester Medical Center in New York who worked on the study ...
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The veinus plexuses in testis drain into testicular vein. On left side the testicular vein drain into left renal vein. On right side the right testicular vein drains into inferior vena cava. The Varicocele is common on left side because the pressure in renal vein is more as compared to inferior vena cava. ...