Before scientist learned how to make a synthetic growth hormone, removing it painstakingly in small amounts from the pituitary glands of human cadavers. a. scientists ...
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TY - JOUR. T1 - Saccharomyces cerevisiae proteins involved in hybrid DNA formation in vitro. AU - Heyer, W. D.. AU - Johnson, A. W.. AU - Norris, D. N.. AU - Tishkoff, D.. AU - Kolodner, R. D.. PY - 1991. Y1 - 1991. N2 - RecA-like activities that can form hybrid DNA in vitro have been identified in a wide variety of organisms. We have previously described the strand exchange protein 1 (SEP1) from the yeast Saccharomyces cerevisiae that can form hybrid DNA in vitro. Purified as an Mr 132 000 polypeptide, recent molecular and immunological studies have now shown that the native form is an Mr 175 000 polypeptide containing strand exchange activity. The gene encoding SEP1 has been cloned and sequenced. The primary sequence failed to reveal any significant sequence homology to other sequences in data base searches. In vivo SEP1 was found to be essential for normal meiosis as cells containing a homozygous insertion mutation in the SEP1 gene failed to sporulate. In order to identify additional factors ...
TY - JOUR. T1 - A regulated MET3-GLC7 gene fusion provides evidence of a mitotic role for Saccharomyces cerevisiae protein phosphatase 1. AU - Black, S. AU - Andrews, P D. AU - Sneddon, A A. AU - Stark, M J. PY - 1995. Y1 - 1995. N2 - Saccharomyces cerevisiae possesses a single essential gene (GLC7) encoding protein phosphatase 1 (PP1). Elevated expression of this gene from the GAL1 promoter is highly detrimental to the cell, causing a growth defect and aberrant bud morphology, which leads to cells exhibiting long, extended buds. By comparison, expression of GLC7 from the weaker MET3 promoter was without significant effect on either growth or morphology. However, repression of GLC7 expression from the MET3 promoter in cells where the MET3-GLC7 fusion was the sole source of PP1 resulted in a mitotic delay. Such cultures showed a massive decrease in the rate of proliferation in conjunction with a significant increase in the proportion of large, budded cells. 46-diamidino-2-phenylindole ...
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I reveal that Saccharomyces cerevisiae Rtt109p promotes genome stability and resistance to DNA-damaging agents, and that it does this by functionally cooperating with the histone chaperone Asf1p to maintain normal chromatin structure. Furthermore, I show that, as for Asf1p, Rtt109p is required for histone H3 acetylation on lysine 56 (K56) in vivo. Moreover I show that Rtt109p directly catalyzes this modification in vitro in a manner that is stimulated by Asf1p. These data establish Rtt109p as a member of a new class of histone acetyltransferases and show that its actions are critical fro cell survival in the presence of DNA damage during S phase. In the second part of this thesis, I reveal that cells deleted for Saccharomyces cerevisiae ESC2 exhibit synthetic sickness when combined with deletions of many genes involved in maintaining genomic stability. Moreover, I show that esc2Δ mutant cells exhibit increased recombination frequency and increased relocalisation of recombination repair protein ...
New Sequences ============= S82971 S82971 1775bp DNA PLN 10-FEB-1997 PEX13=PAS20 [Saccharomyces cerevisiae, Genomic, 1775 nt]. PEX13; Pex13p. SCRGA1 X90950 4305bp DNA PLN 07-FEB-1997 S.cerevisiae rga1 (dbm1) gene. DBM1; pheromone response; RGA1 gene; RGA1 (DBM1); Rga1p (Dbm1p). SCU17262 U17262 3051bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae Pip1p (PIP1) gene, complete cds. PIP1; Pip1p. SCU17263 U17263 2251bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae Pip2p (PIP2) gene, complete cds. PIP2; Pip2p. SCU17264 U17264 1842bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae Pip3p (PIP3) gene, complete cds. PIP3; Pip3p. SCU85960 U85960 1720bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae RNA polymerase II-specific TBP associated factor Taf40p (TAF40) gene, complete cds. TAF40; RNA polymerase II specific TBP associated; factor. SCU86641 U86641 1657bp DNA PLN 08-FEB-1997 Saccharomyces cerevisiae Rim9p (RIM9) gene, complete cds. RIM9; Rim9p. =========== Updated Features/Annotations ============= YSCDYS1 ...
TY - JOUR. T1 - Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae. AU - Higgins, David R.. AU - Prakash, Satya. AU - Reynolds, Paul. AU - Prakash, Louise. PY - 1983. Y1 - 1983. N2 - We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.. AB - We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or ...
TY - JOUR. T1 - Cooperative interactions between pairs of homologous chromatids during meiosis in Saccharomyces cerevisiae. AU - Mell, Joshua Chang. AU - Komachi, Kelly. AU - Hughes, Owen. AU - Burgess, Sean. PY - 2008/6. Y1 - 2008/6. N2 - We report a novel instance of negative interference during Saccharomyces cerevisiae meiosis, where Cremediated recombination between pairs of allelic loxP sites is more frequent than expected. We suggest that endogenous crossover recombination mediates cooperative pairing interactions between all four chromatids of a meiotic bivalent.. AB - We report a novel instance of negative interference during Saccharomyces cerevisiae meiosis, where Cremediated recombination between pairs of allelic loxP sites is more frequent than expected. We suggest that endogenous crossover recombination mediates cooperative pairing interactions between all four chromatids of a meiotic bivalent.. UR - http://www.scopus.com/inward/record.url?scp=49849083414&partnerID=8YFLogxK. UR - ...
In the study, 300 male day-old, Ross 308 broiler chicks were used. Experiment groups were designed as follows: control; 0.1 % Saccharomyces cerevisiae; 0.2 % Saccharomyces cerevisiae; 0.4 % Saccharomyces cerevisiae. The experimental diets were chemically analyzed according to the methods of the Association of Official Analytical Chemists. Twelve groups were obtained, including three replicates for each experimental group. Each replicated group was comprised of 25 chicks, and thus 75 chicks were placed in each experimental group. After 42 days, broiler chickens were slaughtered. Tibiotarsi were weighed with a digital scale, and the lengths were measured with a digital caliper after the drying process. Cortical areas were measured with the ImageJ Image Processing and Analysis Program. A UTEST Model-7014 tension and compression machine and a Maxtest software were used to determine the bone strength of the tibiotarsus. The severity of the tibial dyschondroplasia lesion was evaluated as 0, +1, +2 and ...
ARAUJO, Roberta A.C. et al. Monitoring Saccharomyces cerevisiae populations by mtDNA restriction analysis and other molecular typing methods during spontaneous fermentation for production of the artisanal cachaça. Braz. J. Microbiol. [online]. 2007, vol.38, n.2, pp.217-223. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822007000200006.. An ecological study on Saccharomyces cerevisiae populations in spontaneous fermentation has been conducted in three vats of a cachaça distillery in Minas Gerais, Brazil. Ninety-seven yeast isolates were collected at the beginning, the middle and at the end of the production period, and were identified by standard methods. Differentiation between the indigenous S. cerevisiae strains isolated was performed by mitochondrial DNA (mtDNA) restriction analysis, RAPD-PCR, and PCR fingerprint using an intron splice primer. Analysis of the mtDNA restriction profiles revealed 12 different patterns, 11 corresponding to indigenous yeasts (I to XI) and one (XII) to a ...
Yeast co-immunoprecipitation dataset ; Gavin et al, 2006 YBR119W YPL178W YBR119W YML046W YBR119W YKL012W YBR119W YDR235W YBR119W YIL061C YBR119W YDR240C YBR119W YGR013W YBR119W YMR125W YBR152W YER172C YBR152W YMR240C YBR152W YMR288W YBR152W YPL213W YBR152W YIR009W YBR152W YDL043C YBR152W YJL203W YBR152W YDR473C YBR152W YGR091W YBR152W YGR075C YBR152W YPR178W YBR152W YBR055C YBR152W YHR165C YBR152W YDL030W YBR152W YML049C YBR152W YER029C YBR152W YKL173W YBR152W YDL098C YBR152W YOR308C YDL087C YER172C YDL087C YMR288W YDL087C YHR086W YDL087C YER165W YDL087C YML046W YDL087C YKL012W YDL087C YDR235W YDL087C YHR165C YDL087C YML049C YDL087C YER029C YDL087C YLR275W YDL087C YLR147C YDL087C YIL061C YDL087C YKL173W YDL087C YDR240C YDL087C YGR013W YDL087C YMR125W YDL087C YGR162W YDL087C YLR298C YDL175C YJL050W YDL175C YPL190C YDL175C YOL115W YDR235W YHR086W YDR235W YML046W YDR235W YKL012W YDR235W YIL061C YDR235W YGR013W YDR235W YMR125W YDR240C YHR086W YDR240C YML046W YDR240C YDR235W YDR240C YHR165C YDR240C ...
The Saccharomyces cerevisiae SNF2 gene affects the expression of many diversely regulated genes and has been implicated in transcriptional activation. We report here the cloning and characterization of STH1, a gene that is homologous to SNF2. STH1 is essential for mitotic growth and is functionally distinct from SNF2. A bifunctional STH1-beta-galactosidase protein is located in the nucleus. The predicted 155,914-Da STH1 protein is 72% identical to SNF2 over 661 amino acids and 46% identical over another stretch of 66 amino acids. Both STH1 and SNF2 contain a putative nucleoside triphosphate-binding site and sequences resembling the consensus helicase motifs. The large region of homology shared by STH1 and SNF2 is conserved among other eukaryotic proteins, and STH1 and SNF2 appear to define a novel family of proteins related to helicases. ...
The Sec18 protein (Sec18p) of the yeast Saccharomyces cerevisiae has been identified as a component involved in the vesicular transport of proteins through the secretory and endocytotic pathways. Sec18p is a homologue of the mammalian protein NSF which has been shown, using a number of in vitro transport assay systems and affinity purification procedures, to interact with other proteins in a multisubunit protein complex. This work represents two approaches taken with the aim of identifying proteins that interact with Sec18p in the yeast Saccharomyces cerevisiae. Isolation of protein complexes was first attempted by affinity purification of a tagged version of Sec18p. The protein was C-terminally tagged with a protein A moiety from Staphylococcus aureus containing IgG binding domains. It was hoped that the affinity of protein A for IgG Sepharose could be used to isolate protein complexes that formed in vivo with the Sec18p. Although the fusion construct was shown to be active in vivo, specific ...
Under amino acid starvation conditions, the bakers" yeast Saccharomyces cerevisiae activates a system called "General control of amino acid biosynthesis". Gcn4p, the transcription factor of this system induces the expression of more than 50 genes involved in the different amino acid biosynthetic pathways. In this thesis it could be shown that during simultaneous limitation of amino acids and nitrogen the general control is not activated. More exactly, even a decrease of the Gcn4p activity was detected, which was traced back onto a reduction of the Gcn4 protein amount in the cell. This decrease of the intracellular concentration was caused by translational control of the GCN4 mRNA, which was able to repress even a 2-fold increase of the GCN4 transcription rate. Furthermore during nitrogen starvation conditions no correlation between the stature of eIF-2 phosphorylation and GCN4 expression was observed. For this reason an involvement of the already known mechanism of translation! al regulation of ...
Pulsed electric field (PEF) treatment can be used for non-thermal inactivation of microorganisms. The aim of this paper is to investigate PEF treatment of yeast, Saccharomyces cerevisiae, using three different field waveforms: square; non-oscillating exponential and oscillating exponential. The PEF system used in this paper consists of a pulsed power supply and a parallel-plane metallic electrodes treatment cell located in an air-pressurised chamber. PEF treatment of the yeast was conducted using electric field impulses with magnitudes of 67 kV/cm and 80 kV/cm. The efficacy of the PEF treatment for inactivation of the yeast cells was assessed by comparison of the PEF-treated and untreated yeast populations. Results showed that 3-log10 reduction in the yeast population can be achieved with 100 impulses using all tested waveforms. Amongst all three tested waveforms non-oscillating exponential impulses demonstrated improved PEF performance. The effect of duration of treatment and peak magnitude ...
HOMOLOGOUS recombination is required for the faithful repair of DNA double-strand breaks (DSBs) that arise during normal cellular processes or from exposure of cells to DNA-damaging agents. Central to the process of homologous recombination is the Rad51 protein, which facilitates synapsis and strand invasion into homologous duplex DNA (San Filippo et al. 2008). Rad51 belongs to the RecA family of homologous pairing proteins (Aboussekhra et al. 1992; Basile et al. 1992; Shinohara et al. 1992). Yeast and humans have two RecA homologs: Rad51 and the meiosis-specific Dmc1 (Bishop et al. 1992; San Filippo et al. 2008). In addition, the Saccharomyces cerevisiae RAD55 and RAD57 genes encode proteins with sequence similarity to RecA and Rad51 and are considered to be Rad51 paralogs (Kans and Mortimer 1991; Lovett 1994). Mutation of RAD51, RAD55, or RAD57 confers sensitivity of ionizing radiation (IR) and defects in mitotic and meiotic recombination, indicating that their functions are not redundant ...
TRA1 is an essential gene in Saccharomyces cerevisiae that encodes a 437 kDa protein product. It is a member of a family of key signaling and regulatory molecules that contain a C-terminal phosphatidylinositol-3-kinase (PI3K) domain [1] and is a component of two multisubunit transcriptional regulatory complexes, the SAGA/SLIK and NuA4 complexes, which also contain the histone acetyltransferase enzymes, Gcn5 and Esa1, respectively [2-4]. Tra1 interacts directly with transcriptional activator proteins and is thought to be critical in recruitment of SAGA/SLIK and NuA4 to their target promoters [5-8].. Previously we identified mutations in the C-terminal PI3K domain of Tra1 that showed defects in transcriptional activation, sensitivity to ethanol and the cell wall destabilizing agent calcofluor white and resulted in shortened telomeres [9]. The pattern of changes neither fully mimicked those seen upon disruption of other SAGA/SLIK nor NuA4 components. For example, unlike strains with deletions of ...
During the production of wine and beer, the yeast Saccharomyces cerevisiae can encounter an environment that is deficient in zinc, resulting in a sluggish or a stuck ferment. It has been shown that the Zap1p-transcription factor induces the expression of a regulon in response to zinc deficiency; however, it was evident that a separate regulon was also activated during zinc deficiency in a Zap1p-independent manner. This study discovered the Msn2p and Msn4p (Msn2/4p) transcriptional activator proteins to be an additional control mechanism inducing the stress response during zinc deficiency. Promoter sequence analysis identified the stress response element (STRE) motif, recognized by Msn2/4p, and was significantly enriched in the promoters of genes induced by zinc deficiency. An investigation using genome-wide analyses revealed a distinct regulon consisting of STREcontaining genes whose zinc-responsive expression was abolished in an msn2 msn4 double mutant. An STRE-driven lacZ reporter ...
Yeast cells. Coloured Scanning Electron Micrograph (SEM) of yeast cells, Saccharomyces cerevisiae. This fungus, also known as Bakers or Brewers yeast, consists of single vegetative cells. Some cells can be seen dividing by budding off new cells. Saccharomyces cerevisiae ferments sugar, producing alcohol and carbon dioxide in the process. It has long been used in brewing beer, the production of wine and in baking leavened bread (carbon dioxide causes the dough to rise). Medically, dried Bakers yeast is used as a rich source of vitamin B1, riboflavin and nicotinic acid. Magnification: x125 at 6x7cm size. x200 at 4x5 - Stock Image B250/0646
The effect of yeast (Saccharomyces cerevisiae) on fattening performances of growing cattle is an article from MOJ Ecology & Environmental Sciences for MedCrave Group. The aim of this experiment was to evaluate the yeast on fattening performances of the growing cattle. The experiment was carried out with 179 imported 12-14 months old growing mixed breed bulls (Hereford, Angus, Brangus, and some other crossbreds) that were allocated to control and yeast group according to the breeds and body weight. Experimental diet was formulated with 19 % roughages (alfalfa and wheat straw) containing 13% crude protein. Yeast group was supplemented 40g d-1 live yeast containing 1.23×1011 CFU/g. The study lasted 62 days from May to July. Initial body weight were 393.91±4,43 for control and 395.56±4.45kg for yeast group. After test period, daily gain was similar (1465.85±26.76 vs. 1451.42±34.05g d-1, P|0.05) for the bull receiving the diet without yeast compared to the bulls receiving yeast. Similar results were
Saccharomyces Cerevisiae Yeast Cells Sem Scanning as a 8x6 Glass Mount from CMSP Photo Prints. Fast and safe delivery. Saccharomyces Cerevisiae Yeast Cells. these Microorganisms Fungi are Used to Raise Bread Dough the Yeasts Produce
The Saccharomyces cerevisiae transcription factor Spt20/Ada5 was originally identified by mutations that suppress Ty insertion alleles and by mutations that suppress the toxicity caused by Gal4-VP16 overexpression. Here we present evidence for physical associations between Spt20/Ada5 and three other Spt proteins, suggesting that they exist in a complex. A related study demonstrates that this complex also contains the histone acetyltransferase, Gcn5, and Ada2. This complex has been named SAGA (Spt/Ada/Gcn5 acetyltransferase). To identify functions that genetically interact with SAGA, we have screened for mutations that cause lethality in an spt20Δ/ada5Δ mutant. Our screen identified mutations in SNF2, SIN4, and GAL11. These mutations affect two known transcription complexes: Snf/Swi, which functions in nucleosome remodeling, and Srb/mediator, which is required for regulated transcription by RNA polymerase II. Systematic analysis has demonstrated that spt20Δ/ada5Δ and spt7Δ mutations cause ...
Saccharomyces cerevisiae sudah sejak lama digunakan sebagai starter fermentasi pembuatan roti dan minuman beralkohol. Dalam buku ini, Saccharomyces crervisiae dimanfaatkan sebagai agensia modifikasi dalam pengolahan pangan, kemampuan S. cerevisiae dalam merombak komponen pangan, produk metabolit yang dihasilkan oleh S. cerevisiae, modifikasi terhadap perubahan sifat beberapa produk pangan oleh S. cerevisiae seperti tapioka, tempe, dan modifikasi fermentasi kakao. Pengertian dasar mengenai khamir perlu dipahami oleh mahasiswa yang khususnya mempelajari mikrobiologi pangan, mikrobiologi industri dan teknologi pangan. S.cerevisiae adalah khamir ...
The production of bio-based chemicals, fuels, pharmaceuticals and food additives by microbial fermentation is a rapidly growing field. There is an increasing demand for efficient cell factories that enable the production of biofuels and biochemicals from renewable resources at low and competitive cost. The knowledge of genetics, physiology, biochemistry and large-scale fermentation of bakers yeast Saccharomyces cerevisiae, combined with the advent of genome engineering and recombinant DNA technology makes it a preferred host for many industrial bio-based applications, ranging from biofuels and bulk chemicals to nutraceuticals and pharmaceuticals [1-8]. Furthermore, S. cerevisiae has the advantage of being easy to manipulate genetically with a range of established cloning and vector systems [6, 9].. Production organisms with multi-enzyme pathways often require precise control of the expression level of the associated genes [2, 5, 10]. Besides regulating promoter strength, the copy number of ...
Bakers yeast Saccharomyces cerevisiae is one of the most important and widely used cell factories for recombinant protein production. Many strategies have been applied to engineer this yeast for improving its protein production capacity, but productivity is still relatively low, and with increasing market demand, it is important to identify new gene targets, especially targets that have synergistic effects with previously identified targets. Despite improved protein production, previous studies rarely focused on processes associated with intracellular protein retention. Here we identified genetic modifications involved in the secretory and trafficking pathways, the histone deacetylase complex, and carbohydrate metabolic processes as targets for improving protein secretion in yeast. Especially modifications on the endosome-to-Golgi trafficking was found to effectively reduce protein retention besides increasing protein secretion. Through combinatorial genetic manipulations of several of the newly
Saccharomyces cerevisiae ATCC ® 201390D-5™ Designation: Genomic DNA from Saccharomyces cerevisiae Strain BY4743 (ATCC ® 201390™) Application:
Saccharomyces cerevisiae ATCC ® 201389D-5™ Designation: Genomic DNA from Saccharomyces cerevisiae Strain BY4742 (ATCC ® 201389™) Application:
The Pumilio family (PUF) proteins are conserved among the eukaryotes (42). They bind to specific sequences in the 3′ untranslated region (3′UTR) of target transcripts via their conserved and characteristic PUF domain and thereby inhibit the stability or translatability of these target mRNAs (32, 50). Indeed, the PUF domain appears sufficient for PUF proteins to affect their target transcripts (32, 50). Five PUF proteins, Puf1p to Puf5p, were thought to exist in the budding yeast Saccharomyces cerevisiae (37, 49). A sixth, Puf6p, has recently been reported (9). None are essential (9, 37, 49). One of the yeast PUF proteins, Mpt5p, also known as Htr1p (23), Puf5p (37), or Uth4p (20), promotes replicative life span (3, 20, 21), the number of generations a virgin daughter cell can undergo before becoming senescent. Mpt5p is a robust regulator of ageing, since it also affects life span in a long-lived genetic background (17).. In addition to displaying a short replicative life span, mutants ...
To grow, eukaryotic cells must expand by inserting glycerolipids, sphingolipids, sterols, and proteins into their plasma membrane, and maintain the proper levels and bilayer distribution. A fungal cell must coordinate growth with enlargement of its cell wall. In Saccharomyces cerevisiae, a plasma membrane‐localized protein kinase complex, Target of Rapamicin (TOR) complex‐2 (TORC2) (mammalian ortholog is mTORC2), serves as a sensor and masterregulator of these plasma membrane‐ and cell wall‐associated events by directly phosphorylating and thereby stimulating the activity of two types of effector protein kinases: Ypk1 (mammalian ortholog is SGK1), along with a paralog (Ypk2); and, Pkc1 (mammalian ortholog is PKN2/PRK2). Ypk1 is a central regulator of pathways and processes required for plasma membrane lipid and protein homeostasis, and requires phosphorylation on its T‐loop by eisosome‐associated protein kinase Pkh1 (mammalian ortholog is PDK1) and a paralog (Pkh2). For cell survival under
Septins are a family of eukaryotic GTP-binding proteins that associate into linear rods, which, in turn, polymerize end-on-end into filaments and further assemble into other, more elaborate super-structures at discrete subcellular locations. Hence, septin-based ensembles are considered elements of the cytoskeleton. One function of these structures that has been well-documented in studies conducted in budding yeast Saccharomyces cerevisiae is to serve as a scaffold that recruits regulatory proteins, which dictate the spatial and temporal control of certain aspects of the cell division cycle. In particular, septin-associated protein kinases couple cell cycle progression with cellular morphogenesis. Thus, septin-containing structures serve as signaling platforms that integrate a multitude of signals and coordinate key downstream networks required for cell cycle passage. This review summarizes what we currently understand about how the action of septin-associated protein kinases and their substrates control
1P-022 Saccharomyces cerevisiaeの糖代謝における転写制御ネットワークの予測(遺伝子工学,一般講演)1P-022 Saccharomyces cerevisiaeの糖代謝における転写制御ネットワークの予測(遺伝子工学,一般講演)AN10549378 ...
ERK5 is a mitogen-activated protein (MAP) kinase regulated in human cells by diverse mitogens and stresses but also suspected of mediating the effects of a number of oncogenes. Its expression in the slt2Delta Saccharomyces cerevisiae mutant rescued several of the phenotypes caused by the lack of Slt2p (Mpk1p) cell integrity MAP kinase. ERK5 is able to provide this cell integrity MAP kinase function in yeast, as it is activated by the cell integrity signaling cascade that normally activates Slt2p and, in its active form, able to stimulate at least one key Slt2p target (Rlm1p, the major transcriptional regulator of cell wall genes). In vitro ERK5 kinase activity was abolished by Hsp90 inhibition. ERK5 activity in vivo was also lost in a strain that expresses a mutant Hsp90 chaperone. Therefore, human ERK5 expressed in yeast is an Hsp90 client, despite the widely held belief that the protein kinases of the MAP kinase class are non-Hsp90-dependent activities. Two-hybrid and protein binding studies ...
TY - JOUR. T1 - Subcellular distribution of glutathione and its dynamic changes under oxidative stress in the yeast Saccharomyces cerevisiae. AU - Zechmann, Bernd. AU - Liou, Liang-Chun. AU - Koffler, Barbara E.. AU - Horvat, Lucija. AU - Tomasic, Ana. AU - Fulgosi, Hrvoje. AU - Zhang, Zhaojie. PY - 2011. Y1 - 2011. U2 - 10.1111/j.1567-1364.2011.00753.x. DO - 10.1111/j.1567-1364.2011.00753.x. M3 - Article. VL - 11. SP - 631. EP - 642. JO - FEMS yeast research. JF - FEMS yeast research. SN - 1567-1356. IS - 8. ER - ...
Read "The Genetic Control of Cell Growth and Development in Yeast Saccharomyces cerevisiae: Disturbed Sporulation in Diploids with a Decreased Activity of the Ras/cAMP Signal Transduction Pathway, Russian Journal of Genetics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
In Saccharomyces cerevisiae, disruption of the YCF1 gene increases the sensitivity of cell growth to mercury. Transformation of the resulting ycf1 null mutant with a plasmid harbouring YCF1 under the control of the GAL promoter largely restores the wild-type resistance to the metal ion. The protective effect of Ycf1p against the toxicity of mercury is especially pronounced when yeast cells are grown in rich medium or in minimal medium supplemented with glutathione. Secretory vesicles from S. cerevisiae cells overproducing Ycf1p are shown to exhibit ATP-dependent transport of bis(glutathionato)mercury. Moreover, using beta-galactosidase as a reporter protein, a relationship between mercury addition and the activity of the YCF1 promoter can be shown. Altogether, these observations indicate a defence mechanism involving an induction of the expression of Ycf1p and transport by this protein of mercury-glutathione adducts into the vacuole. Finally, possible coparticipation in mercury tolerance of other ABC
Evolution of multigene families are considered in the review on the example of the PHO gene family encoding the structure of acid phosphatases in the yeast Saccharomyces cerevisiae. Analysis of the...
MOTIZUKI, M., MITSUI, K., ENDO, Y. and TSURUGI, K. (1986), Detection and partial characterization of the chromatin-associated proteases of yeast Saccharomyces cerevisiae. European Journal of Biochemistry, 158: 345-350. doi: 10.1111/j.1432-1033.1986.tb09757.x ...
Cell volume is an important parameter for modelling cellular processes. Temperature-induced variability of cellular size, volume, intracellular granularity, a fraction of budding cells of yeast Saccharomyces cerevisiae CEN.PK 113-7D (in anaerobic glucose unlimited batch cultures) were measured by flow cytometry and matched with the performance of the biomass growth (maximal specific growth rate (μmax), specific rate of glucose consumption, the rate of maintenance, biomass yield on glucose). The critical diameter of single cells was 7.94 μm and it is invariant at growth temperatures above 18.5°C. Below 18.5°C, it exponentially increases up to 10.2 μm. The size of the bud linearly depends on μmax, and it is between 50% at 5°C and 90% at 31°C of the averaged single cell. The intracellular granularity (side scatter channel (SSC)-index) negatively depends on μmax. There are two temperature regions (5-31°C vs. 33-40°C) where the relationship between SSC-index and various cellular parameters ...
Amphiphysins are proteins thought to be involved in synaptic vesicle endocytosis. Amphiphysins share a common BAR domain, which can sense and/or bend membranes, and this function is believed to be essential for endocytosis. Saccharomyces cerevisiae cells lacking the amphiphysin ortholog Rvs161 are i …
Winters MJ, Pryciak PM. Interaction with the SH3 domain protein Bem1 regulates signaling by the Saccharomyces cerevisiae p21-activated kinase Ste20. Mol Cell Biol. 2005 Mar; 25(6):2177-90 ...
Yeast Saccharomyces cerevisiae in vivo Prp8 splicing assay(A) Schematic representation of the two-step splicing pathway (SS, splice site; BS, branch site). Brie
This unit presents detailed protocols for a range of centrifugation‐based subcellular fractionation procedures for the yeast Saccharomyces cerevisiae
TY - JOUR. T1 - Increased stress parameter synthesis in the yeast Saccharomyces cerevisiae after treatment with 4-hydroxy-2-nonenal. AU - Wonisch, Willibald. AU - Hayn, Marianne. AU - Schaur, Jörg. AU - Tatzber, Franz. AU - Kranner, Ilse. AU - Grill, Dieter. AU - Winkler, Rudolf. AU - Bilinski, Tomasz. AU - Kohlwein, Sepp-Dieter. AU - Esterbauer, Hermann. PY - 1997. Y1 - 1997. U2 - 10.1016/S0014-5793(97)00123-3. DO - 10.1016/S0014-5793(97)00123-3. M3 - Article. VL - 405. SP - 11. EP - 15. JO - FEBS letters. JF - FEBS letters. SN - 0014-5793. IS - 1. ER - ...
The covalent attachment of ubiquitin (Ub) to short-lived or damaged proteins is believed to be the signal that initiates their selective degradation. In several cases, it has been shown that the proteolytic signal takes the form of a multi-Ub chain in which successive Ub molecules are linked tandemly at lysine 48 (K-48). Here we show that Ub molecules can be linked together in vivo at two other lysine positions, lysine 29 (K-29) and lysine 63 (K-63). The formation of these alternative linkages is strongly dependent on the presence of the stress-related Ub conjugating enzymes UBC4 and UBC5. Furthermore, expression of Ub carrying a K-63 to arginine 63 substitution in a strain of Saccharomyces cerevisiae that is missing the poly-Ub gene, UBI4, fails to compensate for the stress defects associated with these cells. Taken together, these results suggest that the formation of multi-Ub chains involving K-63 linkages plays an important role in the yeast stress response. In broader terms, these results ...
Background: The yeast Saccharomyces cerevisiae provides intriguing possibilities for synthetic biology and bioprocess applications, but its use is still constrained by cellular characteristics that limit the product yields. Considering the production of advanced biopharmaceuticals, a major hindrance lies in the yeast endoplasmic reticulum (ER), as it is not equipped for efficient and large scale folding of complex proteins, such as human antibodies. Results: Following the example of professional secretory cells, we show that inducing an ER expansion in yeast by deleting the lipid-regulator gene OPI1 can improve the secretion capacity of full-length antibodies up to fourfold. Based on wild-type and ER-enlarged yeast strains, we conducted a screening of a folding factor overexpression library to identify proteins and their expression levels that enhance the secretion of antibodies. Out of six genes tested, addition of the peptidyl-prolyl isomerase CPR5 provided the most beneficial effect on ...
I use this paper in my graduate genetics course. It describes a global screen for synthetic defects involving DNA integrity, which reveals a network of 16 functional modules. The paper illustrates screens based on genetic interactions (in this case, synthetic lethality or fitness defects) and the systems biology used to evaluate the results of such a screen. It also illustrates the use of Saccharomyces cerevisiae as a model system ...
We investigated the relationship in Saccharomyces cerevisiae between the cell cycle start function, CDC25, and two mutants defining components of the cAMP pathway. The thermolabile adenylate cyclase mutant cyr1-2(ts) is phenotypically similar to the temperature-sensitive mutant cdc25(ts) in that both mutants, when shifted to the restrictive temperature, arrest in G1 of the cell cycle and permit the initiation of meiosis and sporulation. The mutant bcy1 [a lesion resulting in a low level of regulatory (R) subunit and a high level of active, catalytic (C) subunit of the CAMP-dependent protein kinase] suppresses the temperature-sensitive phenotype of cyr1-2(ts) and confers an asporogenous phenotype. We found that cdc25(ts) complemented cyr1-2(ts), and, unlike cyr1-2(ts), was not suppressible by bcy1, demonstrating that cyr1 and CDC25 must encode different functions. Also our results indicate that CDC25 does not encode the R subunit of the CAMP-dependent protein kinase. In addition, although the cdc25(ts)
The angelic acid moiety represents an essential modification in many biologically active products. These products are commonly known as angelates and several studies have demonstrated their therapeutic benefits, including anti-inflammatory and anti-cancer effects. However, their availability for use in the development of therapeutics is limited due to poor extraction yields. Chemical synthesis has been achieved but its complexity prevents application, therefore microbial production may offer a promising alternative. Here, we engineered the budding yeast Saccharomyces cerevisiae to produce angelyl-CoA, the CoA-activated form of angelic acid. For yeast-based production of angelyl-CoA we first expressed genes recently identified in the biosynthetic cluster ssf of Streptomyces sp. SF2575 in S. cerevisiae. Exogenous feeding of propionate and heterologous expression of a propionyl-CoA synthase from Streptomyces sp. were initially employed to increase the intracellular propionyl-CoA level, resulting in
Essential for protein sorting in meiotic cell division of Saccharomyces cerevisiae; it binds microtubules. Could also be involved in microtubule-associated motility. Necessary for membrane protein retention in a late Golgi compartment. Interacts with the MVP1 protein.
Saccharomyces cerevisiae is a species of yeast. It is perhaps the most useful yeast, having been instrumental to baking and brewing since ancient times. It is believed that it was originally isolated FROM chado.the skins of grapes (one can see the yeast as a component of the thin white film on the skins of some dark-colored fruits such as plums; it exists among the waxes of the cuticle). It is one of the most intensively studied eukaryotic model organisms in molecular and cell biology, much like Escherichia coli as the model bacterium. It is the microorganism behind the most common type of fermentation. S. cerevisiae cells are round to ovoid, 5-10 micrometres in diameter. It reproduces by a division process known as budding ...
Biosprint® is an active yeast (Saccharomyces cerevisiae MUCL 39885) used for animal feed. Biosprint is authorized by the European Union as feed additive for piglets, cattle for fattening, dairy cows, horses and sows.
New Sequences ============= SCOST5GEN X97545 652bp DNA PLN 31-OCT-1996 S.cerevisiae OST5 gene. 9.5 kD subunit; N-oligosaccharyltransferase; OST5 gene; OST5; 9.5 kD subunit of N-oligosaccharyltransferase. SCU33754 U33754 7676bp DNA PLN 02-NOV-1996 Saccharomyces cerevisiae Vam7p (VAM7), ras-like GTPase (YPT11) and MIG1-like zinc finger protein (MLZ1) genes, complete cds and Sip2p (SPM2) gene, partial cds. VAM7; Vam7p; YPT11; ras-like GTPase; MLZ1; MIG1-like zinc finger protein; SIP2; Sip2p. SCU62252 U62252 1288bp DNA PLN 01-NOV-1996 Saccharomyces cerevisiae mitochondrial inheritance component Mdm12p (MDM12) gene, nuclear gene encoding mitochondrial protein, complete cds. MDM12; mitochondrial inheritance component Mdm12p. =========== Updated Features/Annotations ============= SCCHXIV43 Z46843 43481bp DNA PLN 30-OCT-1996 S.cerevisiae chromosome XIV DNA (43.5 kb). Fk506-bindingadenosine deaminase; cyclase-associated protein; cytoskeleton-associated protein (putative); orf2; orf3; MFA2; mating ...
Saccharomyces cerevisiae is the first eukaryote for which a genome was completely sequenced and it has been studied intensely as a model organism for decades (1). The physiology and genetics for this Crabtree positive, non-motile, unicellular yeast that reproduces by budding are well characterized (7). S. cerevisiae is used routinely in the baking industry and for alcohol fermentations. It is generally recognized as safe (GRAS) and tolerant to a wide range of physiological stresses such as low pH, high ethanol and high osmotic stress (2). It has gained wide-spread use as a host for recombinant protein production (5), pentose utilization capabilities have been developed (2, 4) and yeast have potential for consolidated bioprocessing (CBP) (3, 6 ...
... is the species name of yeast used for making sake. Yeast (called Kobo in Japanese) is the microorganism that is essential for the creation of fermented alcohol. Yeast does this by eating any available sugars in the mash and then converting them to alcohol and carbon dioxide. The yeast also gives of acids which convert to esters, thereby influencing the overall aroma of the sake as well. There are various strains of yeast that can impact the taste and aromas in various ways. Most brewers purchase commercially available sake yeast, however a few breweries will isolate and maintain proprietary strains of sake yeast.. ...
The biological interpretation of genetic interactions is a major challenge. Recently, Kelley and Ideker proposed a method to analyze together genetic and physical networks, which explains many of the known genetic interactions as linking different pathways in the physical network. Here, we extend this method and devise novel analytic tools for interpreting genetic interactions in a physical context. Applying these tools on a large-scale Saccharomyces cerevisiae data set, our analysis reveals 140 between-pathway models that explain 3765 genetic interactions, roughly doubling those that were previously explained. Model genes tend to have short mRNA half-lives and many phosphorylation sites, suggesting that their stringent regulation is linked to pathway redundancy. We also identify pivot proteins that have many physical interactions with both pathways in our models, and show that pivots tend to be essential and highly conserved. Our analysis of models and pivots sheds light on the organization of the
BioAssay record AID 460553 submitted by ChEMBL: Antiaging effect in Saccharomyces cerevisiae K6001 expressing uth1 mutant assessed as extension of replicative life span after 2 days.
Invertase from Saccharomyces cerevisiae that meets the specifications developed at the fifty-seventh meeting was considered to be acceptable because S. cerevisiae is commonly used in the preparation of food. Its use should be limited by Good Manufacturing Practice. ...
Domain architecture and assignment details (superfamily, family, region, evalue) for YDL008W from Saccharomyces cerevisiae SGD. Plus protein sequence and external database links.
TY - JOUR. T1 - Study of immobilized yeast, Saccharomyces cerevisiae, using flow microcalorimetry.. AU - Owusu, Richard. AU - Finch, Arthur. PY - 1985. Y1 - 1985. N2 - When immobilized yeast was exposed to nutrients, the resulting heat effect (dQ/dt; J sec-1) increased exponentially with a doubling time (t2) of 2.2±0.3hr. The half life (dQ/dt) under non-growing conditions with sucrose as substrate was 84hr. The kinetics of the transformation of a series of sugars were characterised. The Michaelis constant (Km) and maximal heat effect, (dQ/dt)max, were determined using two common enzyme kinetics linearization plots. The shapes of the Eadie plots for some sugars are discussed in terms of currently proposed mechanisms of their uptake. AB - When immobilized yeast was exposed to nutrients, the resulting heat effect (dQ/dt; J sec-1) increased exponentially with a doubling time (t2) of 2.2±0.3hr. The half life (dQ/dt) under non-growing conditions with sucrose as substrate was 84hr. The kinetics of ...
Mitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G2 phases are collectively known as interphase. In yeast Cdc28 is the catalytic subunit of the cyclin-dependent kinase (CDK). At G1 phase Cdc28 associates with G1-cyclins Cln1 to Cln3, while B-type cyclins Clb1 to Clb6 regulate Cdc28 during S, G2, and M phases. Cln3/Cdc28 activity is required for cells to pass through start, the commitment point in G1. When Cln3/Cdc28 accumulates more than a certain threshold, SBF (Swi4/Swi6) and MBF (Mbp1/Swi6) are activated, promoting transcription of Cln1, Cln2, and other genes required for S-phase progression. Cln1 and Cln2 interacting with Cdc28 promote activation of B-type cyclin associated CDK, which drives DNA replication and entry into mitosis. Specifically, Cdc28 association with Clb2 and Clb1 promotes entry into mitosis. Cells suffering from DNA damage, ...
Domain architecture and assignment details (superfamily, family, region, evalue) for YMR175W-A from Saccharomyces cerevisiae SGD. Plus protein sequence and external database links.
Abstract. Functional analysis of genes from Saccharomyces cerevisiae has been the major goal after determination of genome sequences. Even though several tools for molecular-genetic analyses have been developed, only a limited number of reliable genetic tools are available to support functional assay at protein level. Epitope tagging is a powerful tool for detecting, purifying, and functional studying of proteins. But systematic tagging systems developed with integration vectors are not available. Here, we have constructed a set of integration vectors allowing a translational fusion of interested proteins to the four different epitope tags (HA, Myc, Flag, and GFP). To confirm function and expression of C-terminal-tagged proteins, we used Cdc11, a component of the septin filament that encircles the mother bud neck and consists of five major proteins: Cdc3, Cdc10, Cdc11, Cdc12, and Sep7. The tagged version of Cdc11 expressed under its endogenous promoter was found to be physiologically functional, ...
The Saccharomyces Cerevisiae Morphological Database(SCMD) is a collection of micrographs of budding yeast mutants. Micorgraphs of mutants with altered cell morphology were taken at Ohya Group, University of Tokyo, from a set of the haploid MATa deleted strains obtained from EUROSCARF. From the micrographs, disruptant cells are automatically extracted by our novel cell-image processing software developed at Morishita Group, University of Tokyo. Heterozygous essential gene deletion set, DAmP collection set, natural yeast strain set and others were analyzed by this software. ...
A 5 nm tomographic slice from a vitreous section of a Saccharomyces cerevisiae cell. (M) is a Mitochondrion and (V) a vacuole. Scale bar, 100 nm. Uppe...
Domain combinations containing the Ubiquitin-like superfamily in Saccharomyces cerevisiae 69_4. Domain architectures illustrate each occurrence of the Ubiquitin-like superfamily.
A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc20, a cell-cycle regulated activator of anaphase-promoting complex/cyclos...
Read "Induction of Hsp104 synthesis in Saccharomyces cerevisiae in the stationary growth phase is inhibited by the petite mutation, Russian Journal of Genetics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
1. The problem of the influence of protein concentration on the kinetic parameters of enzymes has been approached studying the glycolytic enzymes from Saccharomyces cerevisiae in permeabilized cells (in situ). 2. The values of Km and Vmax for the different enzymes were essentially the same in dilute solutions of protein and in concentrated ones (in situ) except in the case of enolase where some differences were observed. 3. Functioning of the whole glycolytic pathway was compared in situ and in vitro measuring the rate of the fermentation of glucose. The rate of fermentation in situ was two fold higher than in vitro and the lag before active fermentation was also much shorter. 4. An unidentified phosphorylated compound, possibly polyphosphate, accumulates during the fermentation of glucose under in situ ...
Domain architectures containing the following SCOP superfamilies _gap_,81296,_gap_ in Saccharomyces cerevisiae M22. Domain architectures illustrate each occurrence of _gap_,81296,_gap_.
A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc15, a protein kinase involves in cytokinesis. These phase and GFPimages ...
Please enjoy this information on EpiCor made available through the generosity of Embrias adoption. Information on EpiCor, yeast fermentate, Saccharomyces cerevisiae.
Gene target information for CTT1 - catalase T (Saccharomyces cerevisiae S288C). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
O estudo do efeito da complexidade estrutural da fonte de nitrogênio no transporte de amônio em Saccharomyces cerevisiae foi realizado cultivando-se o microrganismo em um meio mínimo contendo glicose e fontes de nitrogênio, variando de um simples sal de amônio (sulfato de amônio) a aminoácidos livres (casaminoácidos) e peptídeos (peptona). O transporte de amônio foi avaliado acompanhando-se a entrada do análogo metilamônio, utilizando duas metodologias diferentes: transporte de metilamônio radioativo e efluxo de potássio acoplado ao transporte de metilamônio em células crescidas em diferentes condições de cultivo. A cinética de transporte de amônio é detectada nos meios contendo peptona e amônio e não no meio suplementado com casaminoácidos, e o transporte medido em diferentes fases de crescimento sugere que o processo é mais estável em células crescidas em peptona. Os resultados descritos neste trabalho indicam que a complexidade estrutural interfere com a expressão ...
A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged TEM1. TEM1 is a GTP-binding protein of the ras superfamily involved in te...
... Given the expiration of fossil fuels, conventional energy sources require renewable, efficient,
Tetes tebu merupakan limbah pengolahan gula yang mengandung gula cukup tinggi sehingga sangat potensial dimanfaatkan sebagai media fermentasi. Fermentasi tetes tebu untuk menghasilkan bioetanol menjadi salah satu upaya megurangi jumlah limbah dan memenuhi kebutuhan Bahan Bakar Minyak (BBM) yang semakin meningkat. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pH dan lama fermentasi terhadap produksi bioetanol dari tetes tebu (molase) dengan cara fermentasi menggunakan Saccharomyces cerevisiae. Penelitian ini meliputi proses fermentasi dan pemisahan bioetanol dari media fermentasi. Proses fermentasi dilakukan dengan variasi pH 4, 4,5, dan 5, sedangkan variasi lama fermentasi dilakukan selama 3, 4, 5, dan 6 hari. Bioetanol hasil fermentasi dipisahkan dari media fermentasi dengan metode destilasi fraksinasi dan untuk mengukur kadar bioetanol digunakan metode kromatografi gas. Data yang diperoleh pada setiap perlakuan dianalisis menggunakan analisis varians (ANOVA) dan dilanjutkan ...
Saccharomyces cerevisiae Y12 - Organisms are classified by taxonomy into specified groups such as the multicellular animals, plants, and fungi; or unicellular microorganisms such as a protists, bacteria, and archaea.
The optimum pH range of saccharomyces cerevisiae is typically between four and six in the pH scale, as claimed by a 2005 study conducted by Neelakantam V. Narendranath and Ronan Power, which was...
Author: Stagge, F.; Genre: Thesis; Published in Print: 2010; Title: Etablierung neuartiger Fluoreszenzmarkierungen in der Bäckerhefe Saccharomyces cerevisiae für die hochauflösende Mikroskopie mitochondrialer Proteine.
Pl ss baba mikr b form ban, alkalmas mint sz rakoztat aj nd k, vagy mint tan t eszk z sz l knek s tan roknak. Az leszt gomba vagy s r leszt (Saccharomyces cerevisiae) a sarjadz - vagy leszt gomb k egy fajt ja. A korai id k ta ez a legfontosabb leszt faj - a keny rs t sn l s s rf z sn l haszn lj k. Els k nt a sz l h j n izol lt k (a s t t sz n gy m lcs k mint a...
Du, N., Stillman, B. (2001) Identification and characterization of ORC-interacting protein: Yph1p in Saccharomyces cerevisiae. Clinical Cancer Research, 7 (11). 3793S-3793S. ISSN 1078-0432 ...
Mitogen-activated protein kinase SLT2/MPK1; Serine/threonine MAP kinase; coordinates expression of all 19S regulatory particle assembly-chaperones (RACs) to control proteasome abundance; involved in regulating maintenance of cell wall integrity, cell cycle progression, nuclear mRNA retention in heat shock, septum assembly; required for mitophagy, pexophagy; affects recruitment of mitochondria to phagophore assembly site; plays role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway (484 aa ...
TRANSKRIPTIONSREGULATION (MOLEKULARE GENETIK); ENZYMATISCHE ASPEKTE DES METABOLISMUS (BIOCHEMIE); AMINOSÄURENBIOSYNTHESE + AMINOSÄURENANABOLISMUS (METABOLISMUS); PROTEIN-DNA-WECHSELWIRKUNGEN; DNA-BINDENDE PROTEINE; TRANSKRIPTIONSAKTIVATOREN (MOLEKULARE GENETIK); TRANSCRIPTIONAL REGULATION (MOLECULAR GENETICS); ENZYMATIC ASPECTS OF METABOLISM (BIOCHEMISTRY); AMINO ACID BIOSYNTHESIS + AMINO ACID ANABOLISM (METABOLISM); PROTEIN-DNA INTERACTIONS; DNA BINDING PROTEINS; TRANSCRIPTIONAL ACTIVATORS (MOLECULAR GENETICS ...
The analysis of nrg1Delta demonstrates that Nrg1 plays a role in glucose repression of the SUC2 and GAL genes of S. cerevisiae. Thus, three repressors, Nrg1, Mig1, and Mig2, are involved as the downstream targets of the glucose signaling in S. cerevisiae.
Serine/threonine protein kinase required for cell-type-specific transcription and signal transduction in yeast. It is thought that it is phosphorylated by the ste11 protein kinase and that it can phosphorylate the FUS3 and or KSS1 kinases.
Studies in yeast and mammalian cells have identified the cyclin C-Cdk8p complex as a critical component of gene expression in response to extracellular signals during stress response, differentiation, and development [reviewed in Nemet et al. (2014)]. Work in yeast demonstrated that cyclin C-Cdk8p repress the transcription of many genes involved in diauxic shift and metabolism (Holstege et al. 1998; van de Peppel et al. 2005). Many molecular targets have been identified as important mediators of locus-specific transcriptional controls by cyclin C-Cdk8p. First, in vitro and in vivo studies indicate that the CDK8 complex prevents association between RNA pol II and the core mediator, thus inhibiting transcription (Naar et al. 2002; Knuesel et al. 2009; Ebmeier and Taatjes 2010). Second, the cyclin C-Cdk8p kinase phosphorylates transcriptional activators of stress responsive genes, including Ste12p, Phd1p, Msn2p, and Gcn4p, to stimulate their proteolysis (Nelson et al. 2003; Raithatha et al. 2012). ...
Stock video footage Yeast cells. Coloured scanning electron micrograph (SEM) of cells of bakers yeast (Saccharomyces cerevisiae) from part of a dried, commercial yeast pellet. The fungus consists of ...
Chromosomes of Saccharomyces contain a single linear double-stranded DNA with few repreated sequences. It is believed that these repetitions are caused by the encoding of ribosomal RNA. Less than 5% of sequences have introns. The Saccharomyces cerevisiaegenome was completed in 1996. This species has 6,000 genes. Because of its genetic structure, Saccharomyces cerevisiae is a useful research organism. For example, scientists at the Woolford Laboratory at Carnegie Mellon University have used it to study pathways of ribosome assembly. This in turn has led to a better understanding of the genetic structure not just of this organism, but to certain genetic processes in general. Like other eukaryotes, the 40S ribonucleoprotien contains one 18S rRNA and 32 ribosomal protiens. These and other rRNA structures come from a single 35S transcript. This transcript is synthesizes by polymerase I, while pre-RNA is transcribed by polymerase III. The pre-RNA is later packaged in a 90S RNP. These processes are ...
STRUKTUR UND FUNKTION VON GENEN (GENETIK); SACCHAROMYCES (MYKOLOGIE); TRYPTOPHAN (AMINOSÄUREN, BIOCHEMIE); STRUCTURE AND FUNCTION OF GENES (GENETICS); SACCHAROMYCES (MYCOLOGY); TRYPTOPHANE (AMINO ACIDS, BIOCHEMISTRY ...
The Saccharomyces Genome Database (SGD) provides comprehensive integrated biological information for the budding yeast Saccharomyces cerevisiae.
This page presents information for the proteins of known structure with sequence similarity to the Saccharomyces cerevisiae protein NPL3/YDR432W. The analysis was performed by comparing yeast protein sequences against protein sequences in The RCSB Protein Databank (PDB) using WU-BLAST ...
GUP1 deletion extends mean and maximum replicative lifespan by 32 and 30%, respectively, as well as chronological lifespan. DR-induced maximal replicative lifespan extension is not further increased by GUP1 deletion, while gup1 mutant displayed slightly longer chronological lifespan under DR [2581]. ...
The fission yeast pob1 gene encodes a protein of 871 amino acids carrying an SH3 domain, a SAM domain, and a PH domain. Gene disruption and construction of a temperature-sensitive pob1 mutant indicated that pob1 is essential for cell growth. Loss of its function leads to quick cessation of cellular elongation. Pob1p is homologous to two functionally redundant Saccharomyces cerevisiae proteins, Boi1p and Boi2p, which are necessary for cell growth and relevant to bud formation. Overexpression of pob1 inhibits cell growth, causing the host cells to become round and swollen. In growing cells, Pob1p locates at cell tips during interphase and translocates near the division plane at cytokinesis. Thus, this protein exhibits intracellular dynamics similar to F-actin patches. However, Pob1p constitutes a layer, rather than patches, at growing cell tips. It generates two split discs flanking the septum at cytokinesis. The pob1-defective cells no longer elongate but swell gradually at the middle, eventually ...
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Yeast relevance is related to the easy determination of the link between gene and protein function (Botstein and Fink 1988). Based on the high evoluti...
A.4 Naider, F., Son, C. A., Sargsyan, H., and Becker, J. M., Biochemical and mutagenic analysis of a G protein-coupled receptor: Photocrosslinking of the tridecapeptide alpha-Factor into Ste2p of Saccharomyces cerevisiae reveals contact points between the peptide and its receptor binding site. "3rd International Peptide Symposium/28th European Peptide Symposium", (2004), p.93-95 ...
S cerevisiae RSC complex: an essential, abundant chromatin-remodeling complex of multiple subunits; has homology to SWI/SNF complex; amino acid sequence given in first source
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1LKJ: The solution structure of apocalmodulin from Saccharomyces cerevisiae implies a mechanism for its unique Ca2+ binding property.