We developed databases and tools to display and analyze next-gen sequencing data for a number of organisms and related varieties (mainly for plant small RNAs).
ISOLATE II Plant miRNA Kit is specially designed for the rapid, phenol-free isolation of high quality and highly enriched small RNA (|200nt) without bias, from a wide range of plant cells and tissues.
Plants small to robust, in loose to dense, soft or rigid tufts, dull green to glaucous, sometimes yellowish or yellowish brown above, radiculose proximally. Stems erect, often forked, 1--15 cm, roundish in cross section, cortex of somewhat firm walled cells forming an indistinct to distinct hyaloderm. Leaves erect-appressed to spreading or circinate, occasionally flexuose when dry, spreading when moist, slenderly lanceolate-subulate from a more or less sheathing, non plicate base; lamina 2- or 3-stratose at margins or throughout; margins revolute at shoulders, serrate to serrulate distally, teeth single or paired; costa usually strong and abaxially prominent, sometimes obscure in distal acumen and low in profile, subpercurrent to excurrent; distal laminal cells small, subquadrate to oblong linear, firm-walled, prorulose on both surfaces; basal cells elongate to rectangular or linear, thin-walled or infrequently thick walled toward costa, pale, smooth. Sexual condition dioicous, autoicous or ...
The substantial differences between the biogenesis of animal and plant miRNAs are also reflected in the differences in their requirements for target recognition. It has long been known that plant miRNAs often have targets with perfect [72] or, more frequently, near-perfect [10] complementarity, facilitating relatively simple identification. Canonical plant miRNA target sites are found in 5 UTRs, ORFs and 3 UTRs, as well as within non-protein-coding transcripts, suggesting that all RNA contexts are equally amenable to miRNA-directed regulation in plants. Many of these plant miRNA targets succumb to AGO-catalyzed cleavage when they encounter a cognate miRNA; the characteristic remnants of these cleavage reactions enable molecular confirmation of plant miRNA target predictions in vivo [72-74]. However, not all plant miRNA-target interactions lead to AGO-catalyzed slicing. Some plant miRNA targets have conserved central mismatches embedded within perfectly base-paired regions at the 5 and 3 ends ...
MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancie
Small RNAs have emerged as a means of genetic control that were completely unsuspected only 10 years ago, points out Gregory Hannon, Ph.D., of Cold Spring Harbor Laboratory, who led the research. What we are now finding is that the diversity of small RNAs in many systems is even greater than previously suspected. The new study shows this in Arabidopsis (a model plant), where small RNAs have a major role in controlling the epigenetic organization of the entire genome.. Conventional DNA sequencing methods gave us glimpses of the various classes of small RNAs that exist in plants and animals. However, only with 454 Sequencing have we been able to fully appreciate the extent of small RNA populations and the breadth of biological processes they might affect, adds Dr. Hannon.. The study, entitled, Distinct Catalytic and Non-Catalytic Roles of ARGONAUTE4 in RNA-directed DNA Methylation, which describes a method for obtaining a comprehensive view of the total small RNAs from a single sample, was ...
Attention Due to government legislation, the selling of seeds, herbs and plants from the same location that therapeutic uses are given about plants, would contravene the law. THEREFORE… We do not display or advertise any information of therapeutic uses, of any kind on this web site. We believe that all information on this website is in line with all legal codes of practice ...
RNA silencing pathways control the expression of genes and other DNA loci by the action of small RNA molecules and are found in many eukaryotes. In plants there are a number of RNA silencing pathways, of which RNA-directed DNA methylation (RdDM) is one. In this pathway the small RNA molecules direct DNA methylation, resulting in the down regulation of expression of the target locus. In terms of the mechanism of the pathway it is mostly well characterised but several gaps exist in our knowledge. These relate to its initiation, where it is not known how RdDM targets the correct locus; methylation, where it is unclear how the action of small RNAs triggers methylation; and chromatin modification, where it is unclear how methylated DNA is converted into higher order chromatin modification. These gaps in the pathway raised the possibility of the involvement of novel proteins and so this project aimed to identify and characterise mutants in these proteins. Screening of a library of putative RdDM ...
Fu et al. demonstrate a requirement for the heterochromatin factors CMT and DDM1 in RNA-directed DNA methylation in maize. Plant Cell https://doi.org/10.1105/
One of the most important developments in molecular biology over the past two decades is the emerging picture of a new level of gene regulation under the control of small yet versatile RNAs (1). Small RNA (sRNA) molecules are widely recognized as common and effective modulators of gene expression in many eukaryotic organisms. According to current knowledge, sRNAs are generally divided into several categories, including microRNAs (miRNAs), short interfering RNAs (siRNAs), trans-acting siRNAs (ta-siRNAs), natural antisense transcript siRNAs (nat-siRNAs), and piwi-interacting RNAs (piRNAs) in metazoans (2). In plants, microRNAs (miRNAs) are produced from partially complementary dsRNA precursor molecules (3, 4). These plant miRNAs are the most characterized sRNAs, and the pathways by which they are generated and they play roles in gene regulation have been well documented (2, 3, 5). Several hundred genes encoding miRNAs in plants have been experimentally identified by the traditional Sanger ...
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MicroRNAs (miRNAs) are a class of small RNA molecules that regulate gene expression by inhibiting the protein translation or targeting the mRNA cleavage. They play many important roles in living organism cells; however, the knowledge on miRNAs functions has become more extensive upon their identification in biological
A web-based easy-to-use tool dedicated to the identification of smRNAs with stem-loop shaped precursors (such as microRNAs and short hairpin RNAs) and their target genes/transcripts. PsRobot performs fast analysis to identify smRNAs with stem-loop shaped precursors among batch input data and predicts their targets using a modified Smith-Waterman algorithm. PsRobot integrates the expression data of smRNAs in major plant smRNA biogenesis gene mutants and smRNA-associated protein complexes to give clues to the smRNA generation and functional processes.
Although I am fully convinced of the truth of the views given in this volume, I by no means expect to convince experienced naturalists whose minds are stocked with a multitude of facts all viewed, during a long course of years, from a point of view directly opposite to mine. It is so easy to hide our ignorance under such expressions as plan of creation, unity of design, etc., and to think that we give an explanation when we only restate a fact. Any one whose disposition leads him to attach more weight to unexplained difficulties than to the explanation of a certain number of facts will certainly reject the theory. ...
MicroRNAs (miRNAs) are single stranded non-coding endogenous small RNAs of about 22 nucleotides, which are directly involved in regulating gene expression at post transcr..
Our small ZZ plant for delivery is the ideal choice for inexperienced plant parents. Not only is it adorable, with glossy dark green leaves, its also one of our most low-maintenance houseplants. It thrives in spots with low levels of light, and can go for weeks without watering. Keep it on your desk in the office or o
Meyers, B.C., Axtell, M.J., Bartel, B., Bartel, D.P., Baulcombe, D., Bowman, J.L., Cao, X., Carrington, J.C., Chen, X., Green, P.J., Griffiths-Jones, S., Jacobsen, S.E., Mallory, A.C., Martienssen, R.A., Poethig, R.S., Qi, Y., Vaucheret, H., Voinnet, O., Watanabe, Y., Weigel, D., Zhu, J.K. (2008) Criteria for annotation of plant microRNAs. Plant Cell 20:3186-3190. PMID: 19074682 ...
8 Meyers, B.C., Axtell, M.J., Bartel, B., Bartel, D.P., Baulcombe, D., Bowman, J.L., Cao, X., Carrington, J.C., Chen, X., Green, P.J., Griffiths-Jones, S., Jacobsen, S.E., Mallory, A.C., Martienssen, R.A., Poethig, R.S., Qi, Y., Vaucheret, H., Voinnet, O., Watanabe, Y., Weigel, D., Zhu, J.K. (2008) Criteria for annotation of plant microRNAs. Plant Cell 20:3186-3190. PMID: 19074682 ...
With all four features, a more negative score is associated with a more favorable site. The context score is the sum of the above scores, and the context score percentile is the percentile rank of each site compared to all sites for this miRNA family. Thus a high context score percentile (between 50 and 100) shows that a specific site is more favorable than most other sites of this miRNA. In a gene with multiple sites for one miRNA family, a total context score is calculated as the sum of context scores for the most favorable (most negative) miRNA in this family (as shown on the miRNA family pages [example]). If the context score for any of these sites is positive (unfavorable), its contribution to the total context score is 0. The representative miRNA is the miRNA in this family with the most favorable total context score ...
With all four features, a more negative score is associated with a more favorable site. The context score is the sum of the above scores, and the context score percentile is the percentile rank of each site compared to all sites for this miRNA family. Thus a high context score percentile (between 50 and 100) shows that a specific site is more favorable than most other sites of this miRNA. In a gene with multiple sites for one miRNA family, a total context score is calculated as the sum of context scores for the most favorable (most negative) miRNA in this family (as shown on the miRNA family pages [example]). If the context score for any of these sites is positive (unfavorable), its contribution to the total context score is 0. The representative miRNA is the miRNA in this family with the most favorable total context score ...
TY - JOUR. T1 - Methylation as a crucial step in plant microRNA biogenesis. AU - Yu, Bin. AU - Yang, Zhiyong. AU - Li, Junjie. AU - Minakhina, Svetlana. AU - Yang, Maocheng. AU - Padgett, Richard W.. AU - Steward, Ruth. AU - Chen, Xuemei. PY - 2005/2/11. Y1 - 2005/2/11. N2 - Methylation on the base or the ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and is thought to be crucial for ribosome biogenesis and function. Artificially introduced 2′-O-methyl groups in small interfering RNAs (siRNAs) can stabilize siRNAs in serum without affecting their activities in RNA interference in mammalian cells. Here, we show that plant microRNAs (miRNAs) have a naturally occurring methyl group on the ribose of the last nucleotide. Whereas methylation of rRNAs depends on guide RNAs, the methyltransferase protein HEN1 is sufficient to methylate miRNA/miRNA* duplexes. Our studies uncover a new and crucial step in plant miRNA biogenesis and have profound implications in the function of miRNAs.. AB - ...
Molecular Plant-Microbe Interactions 26:617-625...Jang-Kyun Seo,1 Jianguo Wu,2 Yifan Lii,1 Yi Li,2 and Hailing Jin1...© 2013 The American Phytopathological Society...Small RNAs regulate a multitude of cellular processes, including development, stress responses, metabolism, and maintenance of genome integrity, in a sequence-specific manner. Accumulating evidence reveals that host endogenous small RNAs and small RNA pathway components play important roles in plan...
p,In plants, tasiRNAs form a class of endogenous secondary siRNAs produced through the action of RNA-DEPENDENT-RNA-POLYMERASE-6 (RDR6) upon microRNA-mediated cleavage of non-coding TAS RNAs. In Arabidopsis thaliana, TAS1, TAS2 and TAS4 tasiRNA production proceeds via a single cleavage event mediated by 22nt-long or/and asymmetric miRNAs in an ARGONAUTE-1 (AGO1)-dependent manner. By contrast, tasiRNA production from TAS3 seems to follow the so-called two-hit process, where dual targeting of TAS3, specifically mediated by the 21nt-long, symmetric miR390, initiates AGO7-dependent tasiRNA production. Interestingly, features for TAS3 tasiRNA production differ in other plant species and we show here that such features also enable TAS3 tasiRNA biogenesis in Arabidopsis, and that a single miR390 targeting event is, in fact, sufficient for this process, suggesting that the one-hit model underpins all the necessary rudiments of secondary siRNA biogenesis from plant TAS transcripts. Further results ...
Plant microRNAs (miRNAs) have recently been reported to be involved in the cross-kingdom regulation of specific cellular and physiological processes in animals. However, little of this phenomenon is known for the communication between host plant and insect herbivore. In this study, the plant-derived miRNAs in the hemolymph of a cruciferous specialist Plutella xylostella were identified by small RNAs sequencing. A total of 39 miRNAs with typical characteristics of plant miRNAs were detected, of which 24 had read counts ≥ 2 in each library. Three plant-derived miRNAs with the highest read counts were validated, and all of them were predicted to target the hemocyanin domains-containing genes of P. xylostella. The luciferase assays in the Drosophila S2 cell demonstrated that miR159a and novel-7703-5p could target BJHSP1 and PPO2 respectively, possibly in an incomplete complementary pairing mode. We further found that treatment with agomir-7703-5p significantly influenced the pupal development and egg
植物 miRNA 與 mRNA 完全或接近完全的配對結合後, 會引起目標基因在配對的第十位核酸上發生剪切,進行基因表達的調控。 伴隨第二代定序的進步,利用 PARE* (Parallel Analysis of RNA Ends) 方法, 以降解組定序(Degradome Sequencing)測出剪切位點, 被剪切的 3 片段以 RNA 連接酶連接上 5 adaptor 後, 經反轉錄形成雙股 cDNA, 再以 EcoP 15I** 酵素切位接上 3 adaptor 後往下進行定序分析。 目前此方法已成功應用於阿拉伯芥,水稻等植物的降解組定序上 ...
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Phenol-free Plant RNA Isolation Kit is designed to isolate RNA from plant tissue or cells. This kit eliminates the use of organic solvent extractions by using phenol/chloroform free reagents. Sufficient reagents are provided for 50 extractions. Advantages: Fast and Simple Phenol/chloroform free Exelent recovery Isolate
MicroRNAs (miRNAs) regulate the expression of target mRNAs in plants and animals [1]. Plant miRNA targets have been predicted on… Expand ...
One type of dsRNA is microRNA (miRNA). MicroRNA are basically small pieces of RNA that interact with your genes, essentially stopping certain genes from being expressed. MiRNA exists in human body fluid naturally; however, microRNA also exists in plants, and research has shown that eating the wrong plants may transfer this plant miRNA to humans - with potentially devastating implications.. The study, published in 2011, determined that microRNA from cooked plant foods like rice, wheat and potatoes can in fact collect in your blood and tissue, leading to a number of potential health problems.5 The study further revealed that microRNA remains completely stable after not only cooking, but through the digestion process as well. Most importantly, the researchers found a significant quantity of microRNA in the human body, concluding that:. ...
The characteristics of microbial small RNA transcription are largely unknown, while it is of primary importance for a better identification of molecules with functional activities in the gut niche under both healthy and disease conditions. By performing combined analyses of metagenomic and small RNA sequencing (sRNA-Seq) data, we characterized both the human and microbial small RNA contents of stool samples from healthy individuals and... ...
Plants small to occasionally medium sized, forming tufts or thin mats, glossy green to yellowish-green or golden. Primary stems short and inconspicuous, or conspicuous and creeping and spreading. Secondary stems erect to ascending, or stems and branches spreading or subascending, spirally foliate or complanate; pseudoparaphyllia apparently absent; radiculose below. Leaves ovate- to oblong-lanceolate or obovate-oblong symmetric or asymmetric, apex acuminate, acute or obtuse-apiculate, base slightly decurrent on one or both sides; margins plane to recurved, entire to bluntly or sharply serrate, bordered; costae single, 1/3-3/4 lamina length, sometimes forked along costa; laminal cells either short to ± long hexagonal and walls weak or lax, or cells oval to rhomboidal and walls firm; alar region undifferentiated (occasionally border cells at base numerous and extend part or fully across to costa). Propagula absent or present in leaf axial, short to long cylindrical. Autoicous, rarely dioicous. ...
Relative number of gains and losses of entire miRNA families during metazoan evolution. The relative gain is the number of gained miRNA families compared to the
رشد و متابولیسم گیاه تحت تأثیر انواع محرک‌های زنده و غیرزنده‌ از جمله تنش‌های محیطی قرار می‌گیرد که گیاه از طریق هورمون‌ها به آن‌ها پاسخ می‌دهد. miRNAها، گروهی از RNAهای کوچک غیر کدکننده هستند که برخی از آن‌ها در سیگنالینگ هورمون‌های گیاهی نقش دارند. در این مطالعه با استفاده از تکنیک qRT-PCR، الگوی بیان miR159a,b، miR160، miR167a,b و miR171a، که به‌ترتیب در تنظیم بیان فاکتورهای رونویسی MYB، ARF، ARF، SCL نقش دارند، در دو رقم حساس و متحمل به تنش خشکی در گندم مورد بررسی قرار گرفتند. بررسی میزان شباهت نوکلئوتیدی نشان داد که بیشترین شباهت در هر یک از این خانواده‌ها در ناحیه تولید
komakino fanzine - Giardini di Mir - w/ Mokda: Visti i Giardini di Mirò ieri sera, al circolo degli artisti a roma. - Pieno di ...
PathwayCommons: ctd Pathway. [MIR138-1 results in decreased expression of H2AFX] onclick=removeFacet(PathwayCommons: ctd Pathway/[MIR138-1 results in decreased expression of H2AFX])> PathwayCommons: ctd Pathway [MIR138-1 results in decreased expression of H2AFX] ...
Small RNA-guided gene silencing at the transcriptional and post-transcriptional levels has emerged as an important mode of gene regulation in plants and animals. Thus far, conventional sequencing of small RNA libraries from rice led to the identification of most of the conserved miRNAs. Deep sequencing of small RNA libraries is an effective approach to uncover rare and lineage- and/or species-specific microRNAs (miRNAs) in any organism. In order to identify new miRNAs and possibly abiotic-stress regulated small RNAs in rice, three small RNA libraries were constructed from control rice seedlings and seedlings exposed to drought or salt stress, and then subjected to pyrosequencing. A total of 58,781, 43,003 and 80,990 unique genome-matching small RNAs were obtained from the control, drought and salt stress libraries, respectively. Sequence analysis confirmed the expression of most of the conserved miRNAs in rice. Importantly, 23 new miRNAs mostly each derived from a unique locus in rice genome were
MicroRNAs (miRNAs) and their regulatory functions have been extensively characterized in model species but whether apple has evolved similar or unique regulatory features remains unknown. We performed deep small RNA-seq and identified 23 conserved, 10 less-conserved and 42 apple-specific miRNAs or families with distinct expression patterns. The identified miRNAs target 118 genes representing a wide range of enzymatic and regulatory activities. Apple also conserves two TAS gene families with similar but unique trans-acting small interfering RNA (tasiRNA) biogenesis profiles and target specificities. Importantly, we found that miR159, miR828 and miR858 can collectively target up to 81 MYB genes potentially involved in diverse aspects of plant growth and development. These miRNA target sites are differentially conserved among MYBs, which is largely influenced by the location and conservation of the encoded amino acid residues in MYB factors. Finally, we found that 10 of the 19 miR828-targeted MYBs undergo
Oxidative stress in plants can be triggered by many environmental stress factors, such as drought and salinity. Brachypodium distachyon is a model organism for the study of biofuel plants and crops, such as wheat. Although recent studies have found many oxidative stress response-related proteins, the mechanism of microRNA (miRNA)-mediated oxidative stress response is still unclear. Using next generation high-throughput sequencing technology, the small RNAs were sequenced from the model plant B. distachyon 21 (Bd21) under H2O2 stress and normal growth conditions. In total, 144 known B. distachyon miRNAs and 221 potential new miRNAs were identified. Further analysis of potential new miRNAs suggested that 36 could be clustered into known miRNA families, while the remaining 185 were identified as B. distachyon-specific new miRNAs. Differential analysis of miRNAs from the normal and H2O2 stress libraries identified 31 known and 30 new H2O2 stress responsive miRNAs. The expression patterns of seven
The novel NEBNext Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries.
The novel NEBNext Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries.
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
There is a desperate need for new means to diagnose and treat most human diseases. Extracellular small RNAs provide great promise. In particular, recent findings provide strong evidence for their utility as biomarkers. Furthermore, very recent work shows functional roles for at least a subset of the extracellular small RNAs, which provides an opportunity for their manipulation in the treatment of disease. However, as the field is so recent with only anecdotal evidence in limited systems, there remains a fundamental gap in our knowledge of how wide spread the phenomenon of functional extracellular small RNAs really is and how can its potential be fully realized. This U19 Centers long-term goal is to uncover paradigms of extracellular small RNA function in health and disease and apply those paradigms to clinically relevant settings including biomarker discovery and therapeutic intervention. Members of the Center already have a significant track record in these areas. The objective here is to ...
Epipterygium Lindb., Öfvers. Förh. Kongl. Svenska Vetensk.-Akad. 19: 603. 1862. Plants small, dull or glossy, pale, glaucous-green to pinkish or reddish green in loose tufts; stems forked or simple; rhizoids sparse, reddish brown, lightly papillose. Leaves dimorphic, lateral leaves in 2-3 rows, obovate to broadly elliptic, acute, sometimes decurrent, dorsal leaves in 1-3 rows, smaller and narrower than the lateral leaves, lanceolate to linear-lanceolate acute to shortly acuminate; margins bordered or elimbate, plane, entire to obscurely serrulate above; costa ½ to _ the leaf length in lateral leaves, shorter in dorsal leaves; cells broadly rhombic, rhomboidal or linear, lax or firm, thin-walled, alar cells undifferentiated. Dioicous. Perigonia terminal, bud-like. Setae reddish orange, smooth. Capsules cylindrical to pyriform, inclined to pendent; exothecial cells collenchymatous; stomata superficial; opercula conic-apiculate; annuli rudimentary or revoluble; peristome double, exostome densely ...
R200c, miR205 miR-miR376b, miR381, miR4095p, miR410, miR114 TNBC casesTaqMan qRTPCR (Thermo Fisher Scientific) SYBR green qRTPCR (Qiagen Nv) TaqMan qRTPCR
TY - JOUR. T1 - Elucidation of the RNA Recognition Code for Pentatricopeptide Repeat Proteins Involved in Organelle RNA Editing in Plants. AU - Yagi, Yusuke. AU - Hayashi, Shimpei. AU - Kobayashi, Keiko. AU - Hirayama, Takashi. AU - Nakamura, Takahiro. N1 - Copyright: Copyright 2013 Elsevier B.V., All rights reserved.. PY - 2013/3/5. Y1 - 2013/3/5. N2 - Pentatricopeptide repeat (PPR) proteins are eukaryotic RNA-binding proteins that are commonly found in plants. Organelle transcript processing and stability are mediated by PPR proteins in a gene-specific manner through recognition by tandem arrays of degenerate 35-amino-acid repeating units, the PPR motifs. However, the sequence-specific RNA recognition mechanism of the PPR protein remains largely unknown. Here, we show the principle underlying RNA recognition for PPR proteins involved in RNA editing. The distance between the PPR-RNA alignment and the editable C was shown to be conserved. Amino acid variation at 3 particular positions within the ...
The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgens patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.. Workflow. ...
1. MatzkeM, KannoT, DaxingerL, HuettelB, MatzkeAJ (2009) RNA-mediated chromatin-based silencing in plants. Curr Opin Cell Biol 21: 367-376.. 2. LawJA, JacobsenSE (2010) Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat Rev Genet 11: 204-220.. 3. CastelSE, MartienssenRA (2013) RNA interference in the nucleus: roles for small RNAs in transcription, epigenetics and beyond. Nat Rev Genet 14: 100-112.. 4. MirouzeM, ReindersJ, BucherE, NishimuraT, SchneebergerK, et al. (2009) Selective epigenetic control of retrotransposition in Arabidopsis. Nature 461: 427-430.. 5. TsukaharaS, KobayashiA, KawabeA, MathieuO, MiuraA, et al. (2009) Bursts of retrotransposition reproduced in Arabidopsis. Nature 461: 423-426.. 6. SlotkinRK, VaughnM, BorgesF, TanurdzicM, BeckerJD, et al. (2009) Epigenetic reprogramming and small RNA silencing of transposable elements in pollen. Cell 136: 461-472.. 7. MosherRA, MelnykCW, KellyKA, DunnRM, StudholmeDJ, et al. (2009) Uniparental ...
Lesion mimic mutants are used to elucidate mechanisms controlling plant responses to pathogen attacks and environmental stresses. Here, a new rice (Oryza sativa) lesion mimic mutant, natural blight leaf 3 (nbl3), was identified from T-DNA insertion li
These investigators were studying specific miRNA in plants. They found that such plant derived small RNAs were present in human serum, presumably from ingested plant material.. They also investigated the presence of these plant-derived miRNAs in mice fed with either rice or mouse chow. The rice-fed mice had a higher levels of plant-derived miRNAs compared to the mice fed with chow. And when the researchers added plant miRNAs to the chow, this resulted in higher plant miRNAs in the mouse serum.. Cooking the rice had no effect. That is, the plant-derived miRNAs apparently survived not only the digestive process, but also cooking temperatures.. Apparently what happens is that the plant-derived miRNAs are absorbed by the cells in the intestines. These intestinal cells then package the miRNAs and secrete them into the bloodstream.. What are the implications?. Well, of course, the main implication is that the cooked or uncooked plant material you eat may actually affect the regulation of some of your ...
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
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Genome-wide profiling and functional analyses reveal a network of heterochromatin and small RNA factors that silences repetitive elements and prevents genotoxic stress to ensure fertility.
Quantification of miRNA expression can be performed using a variety of technologies, including NGS and real-time PCR (qPCR). While NGS is the default tool for novel miRNA discovery, commercially available library preparation kits introduce biases and involve tedious procedures. As a result, qPCR has been the most commonly used technology for quantification of miRNA expression - until now. The QIAseq miRNA Library Kit defines a new generation in small RNA sequencing products and includes several distinct features not found in other sequencing kits. With the QIAseq miRNA Library Kit, the power of NGS has been combined with single molecule quantification from Unique Molecular Indices (UMI) to generate the most representative expression data possible. In recent years, NGS has emerged as a highly advanced research tool for both high-throughput miRNA expression analysis and novel miRNA discovery. The QIAseq miRNA Library Kit procedure does not require gel purification, excision and elution, which ...
Aliases : GRMZM2G158839. Description : 35.1.5 not assigned.no ontology.pentatricopeptide (PPR) repeat-containing protein Encodes a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts. pigment defective 247 (PDE247). ...
Except for TAS1 and TAS2 loci, the rapidly evolving PPR-P clade yields the most easily recognizable set of RDR6/DCL4-dependent siRNAs in Arabidopsis. Most of the PPR-P clade transcripts are targeted by multiple miRNAs and/or tasiRNAs, which efficiently drive the cleavage fragments through the RDR6/DCL4 pathway. This very likely reinforces silencing that might be directed via targeting by miR161, miR400, and TAS1/TAS2 tasiRNAs. Given the abundance of the resulting clade-derived siRNAs, it is puzzling why siRNA biogenesis does not spread outside of the clade. Despite 33 nonclade PPR-P transcripts possessing multiple, plausible target sites for sequenced, clade-derived siRNAs, the RDR6/DCL4 system was not directed to this group of transcripts. It is possible that the target prediction algorithm, which we have used successfully with miRNAs and tasiRNAs (Allen et al., 2005; Fahlgren et al., 2007), did not accurately identify prospective targets for this analysis. It is also possible that ...
The ASRP website organizes information about Arabidopsis thaliana small RNAs. This site is funded by NSF grant MCB-1231726. All small RNAs have either been isolated by the ASRP or published by other labs ...
Objective: In this study, tumor-stage predictive abilities of miR21, miR155, miR29a and miR92a were evaluated in rectal cancer (RC). Methods: Expression of miR21, miR155, miR29a and miR92a was detected and quantitated in tumor tissue and in adjacent normal tissue from 40 patients by TaqMan MicroRNA assay. Results: Significant overexpression of miR21, miR155, miR29a and miR92a was observed in RC tissues. While high expression of miR21, miR155 and miR29a in N1-2 and C-D stages presented a potential correlation with N and Duke stages, partial correlation analysis suggested that only miR155 rather than miR21 and miR29a played a greater influencing role. Receiver operating characteristics (ROC) curve analysis showed that miR155 could discriminate N0 from N1-2 with 85.0% sensitivity and 85.0% specificity, N2 from N0-1 with 90.0% sensitivity and 96.7% specificity, and C-D stage from A-B stage with 81.0% sensitivity and 84.2% specificity. Conclusions: Increase in expression of miR155 might represent a novel
The long-term research interest of my laboratory is to understand the molecular mechanisms underlying small RNA metabolism and function. Small RNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), are 20 to 24 nucleotide (nt) RNAs that function as sequence-specific regulators of gene expression at both transcriptional and post-transcriptional levels in eukaryotes.. These small RNAs are involved in numerous cellular processes including development, differentiation, proliferation, apoptosis, and stress responses. We currently employ a combination of genetic, biochemical, cell biological, and genomic approaches to identify and characterize components involved in small RNA metabolism in Arabidopsis thaliana.. ...
microRNAs (miRNAs) are a class of negative regulators that take part in many processes such as growth and development, stress responses, and metabolism in plants. Recently, miRNAs were shown to function in plant nutrient metabolism. Moreover, several miRNAs were identified in the response to nitrogen (N) deficiency. To investigate the functions of other miRNAs in N deficiency, deep sequencing technology was used to detect the expression of small RNAs under N-sufficient and -deficient conditions. The results showed that members from the same miRNA families displayed differential expression in response to N deficiency. Upon N starvation, the expression of miR169, miR171, miR395, miR397, miR398, miR399, miR408, miR827, and miR857 was repressed, whereas those of miR160, miR780, miR826, miR842, and miR846 were induced. miR826, a newly identified N-starvation-induced miRNA, was found to target the AOP2 gene. Among these N-starvation-responsive miRNAs, several were involved in cross-talk among responses to
The International Indian Film Academy brings its annual award show to Florida. Alicia Powell reports. Credit to Reuters. COURTESY IIFA/WIZCRAFT. NO RESALE
QaziTM Total RNA Isolation Kit is a rapid, phenol-free, filter based RNA isolation system. It can be used to isolate total RNA from animal and plant tissue, cultured cells, bacteria, yeast, and viral particles with some modification of the Lysis/Binding Buffer. Plant RNA Isolation Aid is included with QaziTMTotal RNA Isolation Kit (Plant tissues) to... Read More ...
A method of detection and quantification of miRNA free of specific cells of cells, tissue and / or organs in the body fluid for the evaluation of cell death in vivo in different tissues and organs, where in vivo cell death is associated with a disorder of a specific tissue and / or organ comprising: the analysis of a sample of body fluid selected from blood, serum and urine obtained from a subject to determine one or more specific miRNA sequences, wherein said analysis comprises the step of detecting said miRNA with a primer and / or probe that is substantially complementary to a portion of said specific miRNA sequences.
This miR-95-5p miRNA inhibitor is designed to bind to miR-95-5p mature miRNA, inhibiting its effect on the cell. This miRNA inhibitor can be used in experiments to investigate miRNA targets and roles in the cell.
MicroRNAs are ~22nt small RNAs that regulate gene expression post transcriptionally. They have the potential to regulate a large fraction of the human genes, an...