p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
http://fottea.czechphycology.cz/_contents/F12-1-2012-07.pdf Fučíková, K & L.A. Lewis. 2012. Intersection of Chlorella, Muriella, and Bracteacoccus: Resurrecting the genus Chromochloris Kol & Chodat (Chlorophyceae, Chlorophyta). Fottea 12(1):83-93 ...
An INF file is a file used by the Windows family of operating systems; normally in the setup of hardware or the installation of software. INF files tell...
Cell growth is tightly coupled to nutrient availability. The target of rapamycin (TOR) kinase transmits nutritional and environmental cues to the cellular growth machinery. TOR functions in two distinct multiprotein complexes, termed TOR complex 1 (TORC1) and TOR complex 2 (TORC2). While the structure and functions of TORC1 are highly conserved in all eukaryotes, including algae and plants, TORC2 core proteins seem to be missing in photosynthetic organisms. TORC1 controls cell growth by promoting anabolic processes, including protein synthesis and ribosome biogenesis, and inhibiting catabolic processes such as autophagy. Recent studies identified rapamycin-sensitive TORC1 signaling regulating cell growth, autophagy, lipid metabolism, and central metabolic pathways in the model unicellular green alga Chlamydomonas reinhardtii. The central role that microalgae play in global biomass production, together with the high biotechnological potential of these organisms in biofuel production, has drawn attention
The unicellular green alga Chlamydomonas reinhardtii excels at acclimating to a changing environment. We analyzed expression patterns of its three genomes in cells grown under light-dark cycles. Nearly 85% of transcribed genes show differential expression, with different sets of transcripts being up-regulated over the course of the day to coordinate cellular growth before undergoing cell division. Parallel measurements of select metabolites and pigments, physiological parameters and a subset of proteins allow us to infer metabolic events and to evaluate the impact of the transcriptome on the proteome. Among new findings is the observation that Chlamydomonas exhibits low respiratory activity at night and relies instead on fermentative metabolism; we propose that the ferredoxin FDX9 acts as the electron donor to fermentative hydrogenases. The light stress responsive genes PSBS, LHCSR1 and LHCSR3 all show an acute response to light at dawn under abrupt dark-to light transitions. LHCSR3 genes also ...
algae distinguished chiefly by having flagella and a clear green color, their chlorophyll being masked little if at all by other pigments. ...
RNA pyrophosphohydrolase (RppH) catalyzes the removal of pyrophosphate from 5 triphosphorylated RNAs thereby initiating RNA degradation. The enzyme has originally been identified in bacteria but homologs are present in eukaryotes where they are thought to be located in plastids or mitochondria. A homolog of the bacterial RNA pyrophosphohydrolase is present in the unicellular green alga Chlamydomonas reinhardtii suggesting that Chlamydomonas RppH has a role in mRNA degradation in the chloroplast of the alga. The purpose of this project was to determine the localization of the RppH homologue in C. reinhardtii. Localization was investigated using two different constructs, a histidine-tagged version of the Chlamydomonas rppH and a histidine-tagged 5rppH-GFP construct.. A plasmid vector containing Chlamydomonas rppH-6xHN was introduced into C. reinhardtii by nuclear transformation. PCR, RT-PCR, sequencing, and DNA and RNA blotting techniques were used to indentify positive transformants at the DNA ...
The time course of and the influence of light intensity and light quality on the induction of a mitochondrial carbonic anhydrase (CA) in the unicellular green alga Chlamydomonas reinhardtii was characterized using western and northern blots. This CA was expressed only under low-CO2 conditions (ambient air). In asynchronously grown cells, the mRNA was detected 15 min after transfer from air containing 5% CO2 to ambient air, and the 21-kD polypeptide was detected on western blots after 1 h. When transferred back to air containing 5% CO2, the mRNA disappeared within 1 h and the polypeptide was degraded within 3 d. Photosynthesis was required for the induction in asynchronous cultures. The induction increased with light up to 500 mu mol m(-2) s(-1), where saturation occurred. In cells grown synchronously, however, expression of the mitochondrial CA was also detected in darkness. Under such conditions the expression followed a circadian rhythm, with mRNA appearing in the dark 30 min before the light ...
A doença do sarrafo é uma doença neurológica herdada que seja frequentemente fatal. Torna-se geralmente durante a infância, entre as idades de 5 e 10 anos
The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families.Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes. ...
The unicellular green alga Chlamydomonas reinhardtii is an ideal model organism for studies of ciliary function and assembly. In assays for biological and biochemical effects of various factors on flagellar structure and function, synchronous culture is advantageous for minimizing variability. Here, we have characterized a method in which 100% synchronization is achieved with respect to flagellar length but not with respect to the cell cycle. The method requires inducing flagellar regeneration by amputation of the entire cell population and limiting regeneration time. This results in a maximally homogeneous distribution of flagellar lengths at 3 h postamputation. We found that time-limiting new protein synthesis during flagellar synchronization limits variability in the unassembled pool of limiting flagellar protein and variability in flagellar length without affecting the range of cell volumes. We also found that long- and short-flagella mutants that regenerate normally require longer and ...
Algae Details | EE159 | Class: Chlorophyceae | Strain: Hemichloris sp. | Media: Bristol Medium | Origin: Antarctica | Description of Location: | Collection: (1983) | Isolation: | Isolator Number: | Deposition: E.I. Friedmann | Relatives: CCMEE 556 A834-521 | Also Known As: | Notes:
TY - CONF. T1 - TL, OSL, and phototransferred TL in beta-irradiated anion-defective Al2O3. AU - Chernov, V.. AU - Piters, T. M.. AU - Barboza-Flores, M.. PY - 2004/8/1. Y1 - 2004/8/1. U2 - 10.1016/j.radmeas.2004.01.030. DO - 10.1016/j.radmeas.2004.01.030. M3 - Paper. SP - 685. EP - 688. T2 - Radiation Measurements. Y2 - 1 August 2004. ER - ...
To find the area P under the normal probability curve N(mean, standard_deviation) within the interval (left, right), type in the 4 parameters and press "Calculate". The standard normal curve N(0,1) has a mean=0 and s.d.=1. Use -inf and +inf for infinite limits.. ...
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When Venus was used as a GOI with this system, a positive correlation was found between paromomycin resistance and Venus fluorescence, indicating that expression from the two ORFs is coupled. It initially seemed possible that a mechanism such as stop-codon read through (Jackson et al. 2012) might result in expression of the GOI and APHVIII products as a single fusion protein that provides paromomycin resistance. However, this appears not to be the case, because the method works equally well when two or three different stop codons are inserted between the ORFs, and/or the ORFs are placed out-of-frame, and Western blotting detected no such fusion products. Thus, it seems most likely that APHVIII is translated by post-termination reinitiation (Kozak 2007; Skabkin et al. 2013), in which the ribosome remains associated with the mRNA after termination, continues scanning, and reinitiates translation at a downstream (or, occasionally, upstream: Skabkin et al. 2013) AUG. Such events have been well ...
Fusion of green fluorescent protein (GFP) to proteins is a powerful method to investigate dynamic processes in vivo. The green flagellate Chlamydomonas reinhardtii is a model organism for studying the eukaryotic flagella. In this work the GFP-tagging of proteins was employed in order to analyse proteins of the flagellar basal apparatus. Striated fiber assembling (SFA), centrin and deflagellation induced protein of 13 kDa (DIP13) were tagged with GFP at the C-terminal domain. In addition SFA was tagged at the N-terminal domain. The chimeric genes were stably transformed in C. reinhardtii. SFA is the mayor component of the striated microtubule associated fibers (SMAFS). GFP tagged SFA was incorporated into this fibers. N-terminal tagged SFA had similar properties like the wild-type protein. The length of the fibers increased with the strength of expression. The head domain of SFA is essential for fiber formation and photobleaching experiments did not show a pronounced dynamic of the fibers. The ...
View Stock Photo of The Green Algae Chlamydomonas With Its Two Flagella Sem X6000. Find premium, high-resolution photos at Getty Images.
Chromochloris zofingiensis (Dönz) Fucíková & L.A.Lewis 2012: 91, figs 6-10. Published in: Fucíková, C. & Lewis, L.E. (2012). Intersection of Chlorella, Muriella and Bracteacoccus: Resurrecting the genus Chromochloris Kol et Chodat (Chlorophyceae, Chlorophyta). Fottea 12(1): 83-93 ...
Algae Details UTEX Number: 762 Class: Chlorophyceae Strain: Carteria sp. Media: Origin: Bloomington, Indiana USA Description of Location: pond Type Culture: No Collection: Isolation: R.C. Starr (Fall 1955) Isolator Number: Deposition: Relatives: Also Known As: Notes:
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ANTONIUK, Sérgio A. et al. Meningite bacteriana aguda na infância: fatores de risco para complicações agudas e sequelas. J. Pediatr. (Rio J.) [online]. 2011, vol.87, n.6, pp.535-540. ISSN 0021-7557. http://dx.doi.org/10.2223/JPED.2138.. OBJETIVO: Estudo retrospectivo que visa avaliar as complicações neurológicas agudas e sequelas neurológicas das meningites bacterianas agudas na infância, a fim de determinar possíveis sinais de alerta. MÉTODOS: Foram avaliadas crianças (entre 1 mês e 14 anos) internadas entre 2003 e 2006, com meningite bacteriana aguda. RESULTADOS: Dos 44 pacientes incluídos, 17 (38,6%) apresentaram complicações neurológicas agudas, sendo crise convulsiva a mais frequente (31,8%). Os pacientes com complicações neurológicas agudas apresentaram com mais frequência: menor contagem de neutrófilos (p = 0,03), crise convulsiva na admissão (p , 0,01) e S. pneumoniae como agente etiológico (p = 0,01). Os fatores de risco para o desenvolvimento de complicações ...
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Read "Characterization of the LI818 polypeptide from the green unicellular alga Chlamydomonas reinhardtii, Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
In den Jahren 1955 bis 1957 wurden 383 Kulturkartoffelsorten auf ihr Verhalten gegenüber 15 Rassen der Phytophthora infestans geprüft und dabei festgestellt, daß 61 Sorten das Gen R1und 7 Sorten...
Second hand copy. As new. - Contents (partly): Introduction/ Material and Methods/ Results (Water Analyses/ The Phytoplankton of the Waters/ The genus Scenedesmus/ Subgenus Acutodesmus HEGEW./ Subgenus Scenedesmus/ Subgenus Desmodesmus CHOD)/ Discussion of Results/ Acknowledgements/ Summary/ References/ Index/ Tables/ Plates. - Publishers new copies available at EUR 61.00 ...
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https://version-10-5.string-db.org/cgi/network.pl?all_channels_on=1&block_structure_pics_in_bubbles=0&direct_neighbor=1&hide_disconnected_nodes=0&hide_node_labels=0&network_display_mode=svg&network_flavor=evidence&targetmode=proteins&identifier=3055. ...
Rusaini, , and Owens, Leigh (2019) The effect of viral infection on the relationship between the LOS cells and moulting stages of the black tiger prawn (Penaeus monodon). Aquaculture, Aquarium, Conservation and Legislation, 12 (4). pp. 1087-1101. Roberts, David A., Shiels, Laura, Tickle, Julian, de Nys, Rocky, and Paul, Nicholas A. (2018) Bioremediation of aluminium from the waste water of a conventional water treatment plant using the freshwater macroalga Oedogonium. Water, 10 (5). 626. Ram, Ravinesh (2017) Influence of processing techniques on quality and nutritional composition of the tropical sea cucumber Holothuria scabra. PhD thesis, James Cook University. Romano, Nicholas, and Zeng, Chaoshu (2017) Cannibalism of decapod crustaceans and implications for their aquaculture: a review of its prevalence, influencing factors, and mitigating methods. Reviews in Fisheries Science & Aquaculture, 25 (1). pp. 42-69. Ram, Ravinesh, Chand, Roveena V., Forrest, Andrew, and Southgate, Paul C. (2017) ...
The green alga Chlamydomonas reinhardtii possesses a CO2 concentratingmechanism (CCM) which helps in successful acclimationto low CO2 conditions. Current models of the CCM postulate that aseries of ion transporters bring HCO3- from outside the cell to thethylakoid lumen, where the carbonic anhydrase CAH3 dehydratesaccumulated HCO3- to CO2, raising the CO2 concentration forRubisco. Previously, HCO3- transporters have been identified atboth the plasma membrane and the chloroplast envelope, butthe transporter thought to be on the thylakoid membrane hasnot been identified. Three paralogous genes (BST1, BST2, BST3)belonging to the bestrophin family have been found to be upregulatedin low CO2 conditions, and their expression is controlledby CIA5, a transcription factor that controls many CCM genes.YFP fusions demonstrate that all three proteins are located onthe thylakoid membrane, and interactome studies indicate thatthey might associate with chloroplast CCM components. A singlemutant defective in ...
Figure 1: Model of dual function of the pyruvate dehydrogenase complex. In this project, we aim at elucidating the precise molecular working mode of this regulatory principle involving metabolic control of gene expression. By following molecular, genetical, and biochemical approaches in C. reinhardtii, we use in vivo and in vitro techniques to characterize both the RNA binding and the enzymatic forms of DLA2 which appears to shuttle between different protein sub-complexes. A special focus is on the dynamics and composition of these complexes and the role of acetate/acetyl-CoA for balancing both functions of DLA2. This work is complemented by related analyses in cyanobacteria and higher plants, to test for an evolutionary conservation of such a regulatory switch.. A second project is based on recent work from our group that has shown that in C. reinhardtii the translation of the psbD mRNA encoding the D2 protein of PSII is redox-regulated via a single disulphide bridge between the trans-acting ...
Editors note: I first read about this in The Celestine Prophecy (awesome book, btw) back in 1997. A biological research team at Bielefeld University has made a groundbreaking discovery showing that plants can draw an alternative source of energy from other plants. This finding could also have a major impact on the future of bioenergy eventually providing the evidence to show that people draw energy from others in much the same way.. Members of Professor Dr. Olaf Kruses biological research team have confirmed for the first time that a plant, the green alga Chlamydomonas reinhardtii, not only engages in photosynthesis, but also has an alternative source of energy: it can draw it from other plants. The research findings were released this week in the online journal Nature Communications published by the renowned journal Nature.. Flowers need water and light to grow and people are no different. Our physical bodies are like sponges, soaking up the environment. "This is exactly why there are certain ...
The unicellular green alga is becoming an invaluable super model tiffany livingston system in plant biology. presented into our algal appearance strains bring about recombinant protein deposition degrees of up to 0.25?% of the full total cellular protein. Furthermore, in ...
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