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Differential scanning calorimetry, urea denaturation, and X-ray crystallography were combined to study the structural and energetic consequences of refilling an engineered cavity in the hydrophobic core of RNase T1 with CH(3), SH, and OH groups. Three valines that cluster together in the major hydrophobic core of T1 were each replaced with Ala, Ser, Thr, and Cys. Compared to the wild-type protein, all these mutants reduce the thermodynamic stability of the enzyme considerably. The relative order of stability at all three positions is as follows: Val , Ala approximately equal to Thr , Ser. The effect of introducing a sulfhydryl group is more variable. Surprisingly, a Val --, Cys mutation in a hydrophobic environment can be as or even more destabilizing than a Val --, Ser mutation. Furthermore, our results reveal that the penalty for introducing an OH group into a hydrophobic cavity is roughly the same as the gain obtained from filling the cavity with a CH(3) group. The inverse equivalence of the ...
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The 3M Value Index Score (VIS) is a population-centric value measure that complements existing quality and total cost-of-care (TCC) metrics.
A ribonuclease, RNase T-tat, specifically designed to hydrolyze the TAR RNA of HIV-1 virus has been engineered. The protein was made by domain swapping the TAT peptide at the loop 3 position of ribonuclease T1. The RNase T-tat maintains a guanine-specific RNA hydrolytic activity, and characteristically displayed a specific affinity for the TAR RNA of HIV-1. In the in vitro and in vivo assays, the ...
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MPGGGSQEYGVLCIQEYRKNSKVESSTRNNFMGLKDHLGHDLGHLYVESTDPQLSPAVPWSTVENPSMDT 1 - 70 VNVGKDEKEASEENASSGDSEENTNSDHESEQLGSISVEPGLITKTHRQLCRSPCLEPHILKRNEILQDF 71 - 140 KPEESQTTSKEAKKPPDVVREYQTKLEFALKLGYSEEQVQLVLNKLGTDALINDILGELVKLGNKSEADQ 141 - 210 TVSTINTITRETSSLESQRSESPMQEIVTDDGENLRPIVIDGSNVAMSHGNKEVFSCRGIKLAVDWFLER 211 - 280 GHKDITVFVPAWRKEQSRPDALITDQEILRKLEKEKILVFTPSRRVQGRRVVCYDDRFIVKLAFESDGII 281 - 350 VSNDNYRDLANEKPEWKKFIDERLLMYSFVNDKFMPPDDPLGRHGPSLDNFLRKKPIVPEHKKQPCPYGK 351 - 420 KCTYGHKCKYYHPERGSQPQRSVADELRAMSRNTAAKTANEGGLVKSNSVPCSTKADSTSDVKRGAPKRQ 421 - 490 SDPSIRTQVYQDLEEKLPTKNKLETRSVPSLVSIPATSTAKPQSTTSLSNGLPSGVHFPPQDQRPQGQYP 491 - 560 SMMMATKNHGTPMPYEQYPKCDSPVDIGYYSMLNAYSNLSLSGPRSPERRFSLDTDYRISSVASDCSSEG 561 - 630 SMSCGSSDSYVGYNDRSYVSSPDPQLEENLKCQHMHPHSRLNPQPFLQNFHDPLTRGQSYSHEEPKFHHK 631 - 700 PPLPHLALHLPHSAVGARSSCPGDYPSPPSSAHSKAPHLGRSLVATRIDSISDSRLYDSSPSRQRKPYSR 701 - 770 QEGLGSWERPGYGIDAYGYRQTYSLPDNSTQPCYEQFTFQSLPEQQEPAWRIPYCGMPQDPPRYQDNREK 771 - 840 ...
Buy our Natural cow Ribonuclease A protein. Ab52579 is an active full length protein produced in Nativesyntheticaly and has been validated in ChIP. Abcam…
The report generally describes kethoxal, examines its uses, production methods, patents. KETHOXAL market situation is overviewed; kethoxal manufacturers
Compare angiogenin, ribonuclease, RNase A family, 5 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
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Britton RA, Wen T, Schaefer L, Pellegrini O, Uicker WC, Mathy N, Tobin C, Daou R, Szyk J, Condon C. Maturation of the 5 end of Bacillus subtilis 16S rRNA by the essential ribonuclease YkqC/RNase J1. Mol Microbiol. 2007 Jan; 63(1):127-38 ...
Specifically, the enzymes are involved in endonucleolytic cleavage of 3-phosphomononucleotides and 3-phosphooligonucleotides ending in C-P or U-P with 2,3-cyclic phos
View mouse Dicer1 Chr12:104687742-104751952 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
MR imaging is greatly improving care to wounded warriors, many who suffer head injuries from roadside bombs, said Lt. Col. Sean Jersey, M.D., a third-year radiology resident at David Grant Medical Center, Travis Air Force Base, California, who discussed the current and future role of mobile MR imaging in combat and the clinical benefit and types of diseases identified with the modality. Although he was not able to attend RSNA 2012, Lt. Col. Robert Jesinger, M.D., Task Force Medical East Expeditionary Medical Support Squadron radiology flight commander, deployed to Afghanistan in 2012 and led the project to expand MR imaging in a combat war zone. Maj. Samuel Southam, M.D., also contributed to the project.. In addition to brain injuries, trauma, tumors, infectious/inflammatory diseases and congenital disorders are among the afflictions diagnosed and treated by mobile MR imaging on host nationals, military contractors and Special and U.S./NATO Forces and others who otherwise would not have ...
1. U.v. difference spectra show that the anionic surfactant sodium n-dodecyl sulphate unfolds ribonuclease A at pH7.3 and 10.3, but that the cationic surfactant n-dodecyltrimethylammonium bromide does not affect the conformation of the enzyme. 2. Equilibrium-dialysis experiments show that sodium n-dodecyl sulphate binds to ribonuclease A, but no binding of n-dodecyltrimethylammonium bromide could be detected at pH7.3. 3. The enzymic activity of ribonuclease A is unaffected by n-dodecyltrimethylammonium bromide up to a concentration of 0.03m at 25°C. 4. Ultracentrifuge studies support the conclusion that n-dodecyltrimethylammonium bromide does not interact significantly with ribonuclease A. 5. The enthalpy change as measured by microcalorimetry on binding of sodium n-dodecyl sulphate to ribonuclease A is consistent with an exothermic enthalpy of binding occurring simultaneously with an endothermic enthalpy of chain unfolding.. ...
The affinity of RI for ribonucleases is among the highest for any protein-protein interaction; the dissociation constant of the RI-RNase A complex is in the femtomolar (fM) range under physiological conditions while that for the RI-angiogenin complex is less than 1 fM. Despite this high affinity, RI is able to bind a wide variety of RNases A despite their relatively low sequence identity. Both biochemical studies and crystallographic structures of RI-RNase A complexes suggest that the interaction is governed largely by electrostatic interactions, but also involves substantial buried surface area.[3][4] RIs affinity for ribonucleases is important, since many ribonucleases have cytotoxic and cytostatic effects that correlate well with ability to bind RI.[5] Mammalian RIs are unable to bind certain pancreatic ribonuclease family members from other species. In particular, amphibian RNases, such ranpirnase and amphinase from the Northern leopard frog, escape mammalian RI and have been noted to have ...
The Ribonuclease A Superfamily is composed of a group of structurally similar peptides that are secreted by immune cells and epithelial tissues. Several members of the Ribonuclease A Superfamily demonstrate antimicrobial activity, and it has been suggested that some of these ribonucleases play an essential role in host defense. Ribonuclease 7 (RNase 7) is an epithelial-derived secreted peptide with potent broad-spectrum antimicrobial activity. This review summarizes the published literature on RNase 7s antimicrobial properties, structure, regulation, and contributions to host defense. In doing so, we conclude by highlighting key knowledge gaps that must be investigated to completely understand the potential of developing RNase 7 as a novel therapeutic for human infectious diseases.
Ribonuclease Rnase Z complexed with transfer RNA (ribonucleic acid). Computer model showing the structure of bacterial ribonuclease Rnase Z (orange) complexed with synthetic transfer RNA (cyan). From Bacillus subtilis. - Stock Image C035/8314
Ribonuclease H1, Ribonuclease H1, 5-R(*GP*GP*AP*GP*UP*GP*CP*GP*AP*CP*AP*CP*CP*UP*GP*AP*UP*UP*CP*C)-3), 5-D(*DGP*DGP*DAP*DAP*DTP*DCP*DAP*DGP*DGP*DTP*DGP*DTP*DCP*DGP*DCP*DAP*DCP*DTP*DCP*DT)-3 ...
CP000859.PE14 Location/Qualifiers FT CDS_pept 15398..16750 FT /codon_start=1 FT /transl_table=11 FT /locus_tag="Dole_0014" FT /product="putative ribonuclease BN" FT /note="TIGRFAM: putative ribonuclease BN; PFAM: FT ribonuclease BN; KEGG: pin:Ping_1819 putative ribonuclease FT BN" FT /db_xref="EnsemblGenomes-Gn:Dole_0014" FT /db_xref="EnsemblGenomes-Tr:ABW65824" FT /db_xref="GOA:A8ZRQ7" FT /db_xref="InterPro:IPR017039" FT /db_xref="InterPro:IPR036388" FT /db_xref="InterPro:IPR036390" FT /db_xref="UniProtKB/TrEMBL:A8ZRQ7" FT /protein_id="ABW65824.1" FT /translation="MNESKKKRLAARTSGALDFLRTGIWRVRLRELETRERVLVRYARI FT FMIAGREFITDGGPLRASALTFYTVLSLVPVMALAFAVAKGFGLQQTLEKEVLAQFPGQ FT EAVILQMIEYARALLDQTKGGLLAGVGVAVLIWTVIKVLNNIEKSFNAIWANTTPRSMG FT KKFSDYLSIMLVGPLLLILSGSATVLVATQVTAITNKIFFLGWFAPIIMTGLQLLPYLF FT VWLLFSFIYGFMPNTRVPVRSCIFGGVLAGTAFKLLQWAYLIFQVGVSRYNAIYGSFAA FT LPLFLIWMQLSWLVTLFGAELAYAHQSVGHYELEPDSRNISDFLKRIYGLYVAHLLVKT FT FKNGEPPLTADQISARLDLPIRMVNRLLETLSAAGLATQTLSGTGGDPAWQPGRDITDI FT ...
TY - JOUR. T1 - Improved deoxyribozymes for synthesis of covalently branched DNA and RNA. AU - Lee, Christine S.. AU - Mui, Timothy P.. AU - Silverman, Scott K.. PY - 2011/1/17. Y1 - 2011/1/17. N2 - A covalently branched nucleic acid can be synthesized by joining the 2′-hydroxyl of the branch-site ribonucleotide of a DNA or RNA strand to the activated 5′-phosphorus of a separate DNA or RNA strand. We have previously used deoxyribozymes to synthesize several types of branched nucleic acids for experiments in biotechnology and biochemistry. Here, we report in vitro selection experiments to identify improved deoxyribozymes for synthesis of branched DNA and RNA. Each of the new deoxyribozymes requires Mn2+ as a cofactor, rather than Mg2+ as used by our previous branch-forming deoxyribozymes, and each has an initially random region of 40 rather than 22 or fewer combined nucleotides. The deoxyribozymes all function by forming a three-helix-junction (3HJ) complex with their two oligonucleotide ...
NMR study of the cold, heat, and pressure unfolding of ribonuclease A. Related Articles NMR study of the cold, heat, and pressure unfolding of
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The circular-dichroism and proton-magnetic-resonance spectra of complexes of ribonuclease A with dihydrouridine 3′-phosphate, 2′- and 3′-CMP, arabinosyl-3′-CMP, 1-(2-hydroxyethyl)cytosine 2′-phosphate and 1-(3-hydroxypropyl)cytosine 3′-phosphate were studied. Comparison of the results shows that non-additivity of the circular-dichroic spectrum of an enzyme-nucleotide complex may be due to: (a), alteration of the circular dichroic spectrum of the nucleotide under the influence of the asymmetric protein matrix (induced dichroism), and (b) a change in the nucleotide conformation. The contribution of each of the two factors was estimated to calculate the circular-dichoroic spectra of 2′-CMP and 3′-CMP in complex with ribonuclease A. 3′-CMP in this complex was characterized by negative circular dichroism in the long-wavelength absorption band of the nucleotide, whereas 2′-CMP was characterized by positive circular dichroism. Since both nucleotides in the complex are known to be in ...
Summary of Activities: * Construct 17 PCR reactions worked, as verified by Chris Anderson * New oligos for a second restriction site in Construct 17 were ordered * No work on Construct 17 was carried out today * First Zymo small fragment clean up of Construct 18 wobble product carried out * Digestion of Construct 18 was carried out (with full wobble product volume - 50 ul) * Second Zymo small fragment clean up of Construct 18 digestion product carried out * Construct 18 DIGESTED CLEAN stored in blue-stripes box with 50 ul * Digestion of Construct 19 was carried out (with 8 ul of gene synthesis reaction volume) * Small fragment Zymo clean up of Construct 19 digestion product carried out * Gel purification of Construct 19 digestion product not carried out * Analytical gel of Construct 19 pre-digestion was carried out - part was there * Construct 19 DIGESTED CLEAN NOGP stored in blue-stripes box with 10 ul * Construct 19 PRE DIGEST stored in blue-stripes box with 32 ul left To do today: * Clean, ...
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1AFK: Crystal structures of ribonuclease A complexes with 5-diphosphoadenosine 3-phosphate and 5-diphosphoadenosine 2-phosphate at 1.7 A resolution.
1mgwA:56-137 1mgrA:56-137 1uckB:11-92 1i70A:11-92 2sarA:11-92 1ucjB:11-92 1lniB:11-92 1ay7A:11-92 1t2hB:11-92 1boxA:11-92 1uclA:11-92 1rgeB:11-92 1t2iA:11-92 1c54A:11-92 1rsnB:11-92 1gmqA:11-92 1uciA:11-92 1sarB:11-92 1gmpA:11-92 1rgfA:11-92 1rggB:11-92 1rghB:11-92 1i8vB:11-92 1gmrB:11-92 1ynvX:11-92 1py3B:79-159 1pylA:79-159 2rbiB:72-161 1goyA:72-161 1gouB:72-161 1govA:72-161 1bujA:72-161 1baoB:67-156 1bsdA:67-156 1banB:67-156 1brhA:67-156 1brgC:67-156 1brkC:67-156 1bnsA:67-156 1bnfB:67-156 1bgsB:67-156 1bnjB:67-156 1bsaB:67-156 1bsbC:67-156 1b3sB:67-156 1x1wB:67-156 1bniB:67-156 1b2xB:67-156 1b2zA:67-156 1bscC:67-156 1bseB:67-156 1x1yB:67-156 1briC:67-156 1b2uC:67-156 1b27C:67-156 1b20B:67-156 1bnr :67-156 1b2sC:67-156 1yvs :67-156 1brsC:67-156 1brjC:67-156 1bneA:67-156 1bngC:67-156 1a2pA:67-156 1x1uB:67-156 1fw7A:67-156 1rnbA:67-156 1b21C:67-156 1x1xB:67-156 1brnM:67-156 1b2mA:46-129 1i0vA:46-129 1rls :46-129 1fysA:46-129 1bviB:46-129 1i2eA:46-129 2hohD:46-129 3rnt :46-129 6gsp :46-129 4gsp ...
Altman, S., Baer, M.F., Bartkiewicz, M., Gold, H., Guerrier-Takada, C., Kirsebom, L.A., Lumelsky, N., Peck, K.: Gene, 82, 63-64 (1989) (Review)PubMedCrossRefGoogle Scholar ...
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