rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" , ,rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/8117", ,dc:creator,Harris, Christopher M.,/dc:creator, ,dc:contributor,Molla, Gianluca,/dc:contributor, ,dcterms:issued,1999,/dcterms:issued, ,dc:contributor,Pilone, Mirella S.,/dc:contributor, ,foaf:homepage rdf:resource="http://localhost:8080/jspui"/, ,dc:contributor,Harris, Christopher M.,/dc:contributor, ,dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/, ,dc:contributor,Motteran, Laura,/dc:contributor, ,dcterms:bibliographicCitation,First publ. in: Flavins and flavoproteins 1999 : ...
A fatal case of meningitis due to Rhodotorula mucilaginosa in a 28 year-old HIV-negative male with a history of Hodgkin lymphoma who underwent salvage chemotherapy is presented. Reviewing the literature we identified 13 cases with central nervous sys
Article Examination of Pb|sup|2+|/sup| bio-sorption onto |italic|Rhodotorula mucilaginosa|/italic| using response surface methodology. With the rapid industrial development, wastewater has been a risk for environmental contamination. We aimed ...
Introduction. Lignocellulosic material containing cellulose, hemicellulose, and lignin is an abundant renewable organic resource that can be used for the production of energy and biochemicals (Weber et al., 2010). The conversion of both the cellulose and hemicellulose fractions for producing biochemicals is being intensively studied. Between 23% to 40% of the lignocellulosic biomass consists of hemicellulose, the main component being xylose in most hardwoods and annual plants (Lee et al., 1979). Whereas the fermentation of glucose can be carried out efficiently with the common brewers yeast (Saccharomyces cerevisiae), the bioconversion of the pentose fraction (xylose and arabinose) presents a challenge since it is not metabolized by this species. Over the past decades, numerous studies have been carried out on various aspects of D-xylose bioconversion (Winkelhausen and Kuzmanova, 1998; Townsend and Howarth, 2010). D-xylose can be used by bacteria, yeasts and fungi through different pathways ...
Glutathione reductase (GR) catalyzes the reduction of glutathione disulfide, which helps to maintain a cellular reducing environment during stress in organisms. However, GR of polar yeast has not...
Lignocelluloses as agricultural, industrial, forest residuals, food-processing waste or paper wastes have the potential to serve as low-cost and abundant source of raw materials for the production of different biochemicals ...
The l-mandelate dehydrogenase (l-MDH) from the yeast Rhodotorula graminisis a mitochondrial flavocytochrome b2 which catalyses the oxidation of mandelate to phenylglyoxylate coupled with the reduction of cytochrome c. We have used the N-terminal sequence of the enzyme to isolate the gene encoding this enzyme using the PCR. Comparison of the genomic sequence with the sequence of cDNA prepared by reverse transcription PCR revealed the presence of 11 introns in the coding region. The predicted amino acid sequence indicates a close relationship with the flavocytochromes b2 from Saccharomyces cerevisiae and Hansenula anomala, with about 40% identity to each. The sequence shows that a key residue for substrate specificity in S. cerevisiae flavocytochrome b2, Leu-230, is replaced by Gly in l-MDH. This substitution is likely to play an important part in determining the different substrate specificities of the two enzymes. We have developed an expression system and purification protocol for recombinant ...
Onychomycosis is a common superficial fungal infection, which usually caused by dermatophytes, yeast and non-dermatophytic moulds. Recently, we isolated a Rhodotorula minuta isolate from a 15-year-old immunocompetent girl student in Hangzhou (China) that was identified using microscopy, culture morphology, histological diagnosis, API 20C AUX Yeast Identification Kit and sequencing of the Internal Transcribed Spacer region. In vitro, antifungal susceptibility tests showed that this yeast isolate was susceptible to low concentrations of amphotericin B, itraconazole, voriconazole and 5-flvoriconaz but that it appeared to be dose-dependent susceptible to fluconazole(MIC = 16 μg/ml). Furthermore, the effective result of therapy with itraconazole against R. minuta was consistent with that of susceptibility tests. ...
The feasibility of heterotrophic-phototrophic symbioses was tested via pairing of yeast strains Cryptococcus curvatus, Rhodotorula glutinis, or Saccharomyces cerevisiae with a sucrose-secreting cyanobacterium Synechococcus elongatus. The phototroph S. elongatus showed no growth in standard BG-11 medium with yeast extract, but grew well in BG-11 medium alone or supplemented with yeast nitrogen base without amino acids (YNB w/o aa). Among three yeast species, C. curvatus and R. glutinis adapted well to the BG-11 medium supplemented with YNB w/o aa, sucrose, and various concentrations of NaCl needed to maintain sucrose secretion from S. elongatus, while growth of S. cerevisiae was highly dependent on sucrose levels. R. glutinis and C. curvatus grew efficiently and utilized sucrose produced by the partner in co-culture. Co-cultures of S. elongatus and R. glutinis were sustained over 1 month in both batch and in semi-continuous culture, with the final biomass and overall lipid yields in the batch co-culture
The presence of very reactive C1-C4 molecules adversely affects the quality bio-oils produced from thepyrolysis of lignocellulosic materials. In this paper a scheme to produce lipids with Cryptococcus curvatus from the carboxylic acids in the pyrolytic aqueous phase collected in fractional condensers is proposed. The capacities of three oleaginous yeasts C. curvatus, Rhodotorula glutinis, Lipomyces starkeyi to ferment acetate, formate, hydroxylacat-aldehyde, phenol and acetol were investigated. While acetate could be a good carbon source for lipid production, formate provides additional energy and contributes to yeast growth and lipid production as auxiliary energy resource. Acetol could slightly support yeast growth, but it inhibits lipid accumulation. Hydroxyacetaldehyde and phenols showed high yeast growth and lipid accumulation inhibition. A pyrolytic aqueous phase with 20 g/L acetate was fermented with C. curvatus, after neutralization and detoxification to produce 6.9 g/L dry biomass and ...
Marchant, R (1969) The fine structure and development of the fructification of Lycoperdon perlatum. Transactions of the British Mycological Society, 53 . pp. 63-68. [Journal article] Smith, DG and Marchant, R (1969) Unbalanced cell-wall synthesis in chloramphenicol-grown Rhodotorula glutinis. Antonie van Leeuwenhoek, 35 . pp. 113-119. [Journal article] Smith, DG and Marchant, R (1969) The use of ferritin-conjugated antibody for an electron microscopic investigation of hyphal growth in Fusarium culmorum. Microbios, 2 . pp. 177-183. [Journal article] Smith, DG, Marchant, R, Maroudas, NG and Wilkie, D (1969) A comparative study of the mitochondrial structure of petite strains of Saccharomyces cerevisiae. Journal of general Microbiology, 56 . pp. 47-54. [Journal article] ...
Itraconazole, fluconazole, voriconazole and posaconazole - the mechanism of action of itraconazole is the same as the other azole antifungals: it inhibits the fungal cytochrome P450 oxidase-mediated synthesis of ergosterol.. Fluconazole is active against most Candida species, with the absolute exception of Candida krusei and partial exception of Candida glabrata and a small number of isolates of Candida albicans, Candida tropicalis, Candida parapsilosis and other rare species. It is also active against the vast majority of Cryptococcus neoformans isolates. It is active against many other yeasts including Trichosporon beigelii, Rhodotorula rubra, and the dimorphic endemic fungi including Blastomyces dermatitidis, Coccidioides immitis, Histoplasma capsulatumand Paracoccidioides brasiliensis. It is less active than itraconazole against these dimorphic fungi. It is not active against Aspergillus or Mucorales. It is active against skin fungi such as Trichophyton. Increasing resistance in Candida ...
Die Biosynthese der Karotenoide im Hefepilz Rhodotorula gracilis III. Der Einfluss verschiedener Stickstoffquellen, des pH und des Verhältnisses von Stickstoff zu Kohlenstoff im Nährboden auf die Bildung der ...
Background: Rothia spp. are gram positive bacteria which are known to cause infections in the immune compromised host. Literature is limited on the epidemiology and clinical significance of Rothiabacteremia. Methods: We reviewed medical records of all patients with blood cultures positive from 2006-2014. Descriptive analysis was performed, as well as comparative analysis of neutropenic patients (absolute neutrophil count ≤ 1000 /μL) at the time of bacteremia with non-neutropenic patients at the time of bacteremia. Fischer`s exact tests were used for comparisons of proportions and medians, respectively, with p-values ,0.05 considered statistically significant. Results: 29 patients with Rothia bacteremia were identified. Median age was 58 years (range 27-73), with no significant gender difference (p=0.94). Charlson co-morbidity score of 4 or greater was found in 20 (69%) of patients; 20 (69%) patients had a hematologic malignancy or bone marrow transplant. While there were 15 deaths, only 1 was ...
Petri dish culture of a yeastlike organism (fungus), Rhodotorula sp, known as a red yeast to the production of a carotenoid pigment. This Fungus is a saprophytic organism, meaning that it acquires nutrition from dead or decaying tissue, either plant or animal. As a food spoilage organism it can contaminate domestic & industrial food. Rhodotorula sp appears in two forms, capsulated & noncapsulated. The noncapsulated variety is seen here. The capsulated version produces a slimy mucilage, the surface becoming smooth & shiny. Its function is uncertain, but in pathogenic species it is associated with increased virulence. - Stock Image B250/0197
Introduction & Objective: In recent years, microorganisms have been applied as biocatalysts for making pharmaceutically natural products. Microbial biotransformation of caffeine suggests a dual approach for biodegradation of toxic caffeine from polluted environments and a method for the production of medically and pharmaceutically valuable dimethylxanthines. The present work describes the identification of ...
Las levaduras son hongos que forman sobre los medios de cultivo colonias pastosas, constituidas en su mayor parte por células aisladas que suelen ser esféricas, ovoideas, elipsoideas o alargadas. Las levaduras son organismos aerobios y aunque muchas especies son fermentadoras, otras no lo son, como los géneros Cryptococcus y Rhodotorula. Las levaduras suelen fermentar unos pocos glúcidos, principalmente hexosas y disacáridos ...
Figure 1. 1a. Bilateral peribronchial infiltration on chest radiogram revealed at admission. 1b. Bilateral diffuse consolidation and ground-glass opacity on thorax computed tomography. He deteriorated suddenly on the fifteenth day accompanied by fever and his dyspnea and oxygen need were aggravated again. Chest radiograph didnt show any new findings. Complete blood count revealed a hemoglobin level of 8.4 g/dL, white blood cell of 10.6x109 /L, platelet count of 113 x109 /L. There was no severe neutropenia but meropenem was started owing to underlying risk factors. Rothia mucilaginosa was isolated on blood culture; as it was susceptible to meropenem and vancomycin; vancomycin was restarted. After five days his dyspnea and oxygen requirement began to diminish. There was no growth on the blood culture obtained 48 hours after the beginning of treatment. The patient was discharged after receiving 14 days of meropenem and vancomycin therapy after a negative blood culture result.. DISCUSSION. Rothia ...
Interaction of acidithiobacillus ferrooxidans, rhizobium phaseoli and rhodotorula sp. in bioleaching process based on lotka-volterra model . Dongwei Li ;Electronic Journal of Biotechnology 2016, 22. Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Recent research on the flavoenzyme D-amino acid oxidase from Rhodotorula gracilis (RgDAAO) has revealed new, intriguing properties of this catalyst and offers novel biotechnological applications. Among them, the reaction of RgDAAO has been exploited in the analytical determination of the D-amino acid content in biological samples. However, because the enzyme does not oxidize acidic D-amino acids, it cannot be used to detect the total amount of D-amino acids. We now present the results obtained using a random mutagenesis approach to produce RgDAAO mutants with a broader substrate specificity. The libraries of RgDAAO mutants were generated by error-prone PCR, expressed in BL21(DE3)pLysS Escherichia coli cells and screened for their ability to oxidize different substrates by means of an activity assay. Five random mutants that have a modified substrate specificity, more useful for the analytical determination of the entire content of D-amino acids than wild-type RgDAAO, have been isolated. With ...
Mandelic acid (MA), an important component in pharmaceutical syntheses, is currently produced exclusively via petrochemical processes. Growing concerns over the environment and fossil energy costs have inspired a quest to develop alternative routes to MA using renewable resources. Herein we report the first direct route to optically pure MA from glucose via genetic modification of the L-phenylalanine pathway in E. coli. The introduction of hydroxymandelate synthase (HmaS) from Amycolatopsis orientalis into E. coli led to a yield of 0.092 g/L S-MA. By combined deletion of competing pathways, further optimization of S-MA production was achieved, and the yield reached 0.74 g/L within 24 h. To produce R-MA, hydroxymandelate oxidase (Hmo) from Streptomyces coelicolor and D-mandelate dehydrogenase (DMD) from Rhodotorula graminis were co-expressed in an S-MA-producing strain, and the resulting strain was capable of producing 0.68 g/L R-MA. Finally, phenylpyruvate feeding experiments suggest that HmaS is a
Im currently doing a feasibily report on the use of Oleaginous yeast in the manufacturing of acidic protease for the used in fodd product and have a few questions that I hope you might be able to help me. 1. Can you recommend the type of Oleaginous yeast avaiable for used in the production of the protease. 2. How do I used it in the manufacturing process using waste cheese whey as a media. Thank you very much J Y Kee ...
SUMMARY: When eight strains of the oleaginous yeasts Candida curvata, Lipomyces starkeyi, Rhodosporidium toruloides and Trichosporon cutaneum were starved of carbon after having accumulated lipid up to 34% of their biomass, the lipid was readily converted to new biomass in all cases except the two strains of L. starkeyi. When C. curvata D was grown in a two-stage chemostat with the second stage as a carbon-starvation vessel (but containing NH+ 4) biosynthesis of new biomass reached 1.9 ± 0.2 g per g lipid utilized. Experiments in a single-stage chemostat undergoing transition from lipid accumulation (nitrogen-limited medium) to carbon-starvation conditions showed that the lipid in C. curvata was rapidly mobilized. When the lipid was pre-labelled with 14C, a transitory pool of rapidly metabolizable non-lipid material appeared within 1.5 h of the initiation of starvation. Rates of lipid loss indicated that initiation of lipid degradation occurred immediately carbon was lost from the external medium.
Principal Investigator:KONNO Ryuichi, Project Period (FY):1993 - 1995, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Kidney internal medicine
General Information: Rothia mucilaginosa is a gram-positive, cocci-shaped bacterium that inhabits the oral cavity. The bacterium is considered an opportunistic pathogen and has been associated with endocarditis, meningitis, and peritonitis. ...
D-Amino Acid Oxidase Inhibitor III, AS057278 - CAS 402-61-9 - Calbiochem CAS 402-61-9 - Find MSDS or SDS, a COA, data sheets and more information.
D-Amino Acid Oxidase Inhibitor III, AS057278 - CAS 402-61-9 - Calbiochem CAS 402-61-9 - Find MSDS or SDS, a COA, data sheets and more information.
Oleaginous yeast Rhodosporidium toruloides is an excellent microbial lipid producer using carbohydrates as the feedstock [1]. Therefore, it is important to develop molecular biology tools to further manipulate this microorganism. Extraction of high-quality RNA from R. toruloides is particular challenging due to high level of polysaccharides, lipids and other secondary metabolites [2]. To obtain an optimal protocol for RNA extraction from R. toruloides, four methods, including guanidine thiocyanate [3], glass beads-hot acid phenol, glass beads-TRIzol, and modified RNAiso, were evaluated. RNA quality was assessed using UV absorbance (A260/A280 and A260/A230), agarose gel electrophoresis, reverse transcription (RT)-PCR reactions, and Gel-Pro analyzer 4.5. Large differences in RNA yield and quality among protocols were found. The optimum method was modified RNAiso method, where RNA was isolated using liquid nitrogen-RNAiso with salt precipitation and the addition of PVP andβ-mercaptoethanol. This ...
Objectives To target a carotenoid biosynthetic gene in the oleaginous yeast Rhodosporidium toruloides by using the Agrobacterium-mediated transformation (AMT) method.
Kocho and Bula are fermented product of Enset (Ensete ventricosum). It is the staple food for 20 million people in Ethiopia. The aim of study was to isolate, identify and characterize yeast species from fermented kocho and bulla by using Biolog Micro station. 300 Kocho samples were collected from Angacha District. 0.1ml of serially diluted samples were Streaked on yeast pepton dextrose agar and incubated at 280C. Pure yeast colony inoculum were prepared at 9ml distilled water at 49% +2 turbidometer and transferred in to YT micro plate. Incubated for 24-72 hours at 28oC and micro plate reading were carried out using MicroLog 3 Software version. 4.20.05. Seven yeast species were identified from study samples. Biolog Micro station 100% probability and |0.5 Similarity read identify Cryptococcus albidus Var aerus, Guilliermondella selenospora, Rhodotorula acheniorum and Trichosporon beigelii. 99% Cryptococcus terreus A, 98% Candida zylandase, 86% Kluyveramyces delphensis respectively. Characterization of
COVID-19 (short for "Coronavirus Disease 2019) is the disease caused by the novel coronavirus, SARS-CoV-2. Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. COVID-19 represents a particular challenge to people with serious chronic medical conditions such as diabetes. To keep our community as informed as possible, the American Diabetes Association is partnering with the Chan Zuckerberg Initiatives Meta to highlight the latest research developments at the intersection of COVID-19 and diabetes. ...
The killer effect of 37 species of Candida, Cryptococcus, Hansenula, Pichia, Rhodotorula, Saccharomyces, and Trichosporon on 100 Candida albicans isolates of human and animal origin was studied. All of the C. albicans cultures were sensitive to one or more killer yeasts. The factors affecting the killer phenomenon on C. albicans were investigated for realizing a simple system for the differentiation of the 100 C. albicans isolates. By using this system, it was possible to differentiate up to 512 isolates of C. albicans according to their susceptibility to the killer effect of nine selected killer yeasts. The use of this method as an epidemiological marker in the case of presumptive nosocomial infections due to C. albicans is also reported. ...
The ability to utilize nitrate as a sole source of nitrogen is an important taxonomic indicator of some species. Previous research by Hipkin has grouped yeasts on the basis of nitrate utilization, with some species being able to assimilate nitrate ( Brettanomyces, Candida, Hansenula, Pachysolen, and Rhodotorula ) while others are not ( Kluyveromyces, Pichia, Saccharomyces and Schizosaccharomyces ). (8) Nitrate assimilation is defined simply as the utilization of a nitrogen source by a microorganism in the presence of oxygen. A positive reaction is indicated by the presence of growth or the use of a pH indicator in the medium. The indicator method is a modification of the Wickerham method that was devised by Adams and Cooper. (7) The use of this method is easier to read than conventional techniques, yet it is equally as reliable. (7) Additionally, this method is not affected by carry-over of the inoculum. Hardy Diagnostics Modified Nitrate Assimilation Agar contains a yeast carbon base composed ...
The level of microbiological contamination with yeasts and moulds in samples of goat milk collected in spring, summer and autumn season was evaluated. Yeasts are present in a larger number in the analyzed goat milk with values between 1.9-3.42 log CFU/ml, contrast to molds that had a range of mean values between 1.47-3.02 log CFU/ml. Of the milk samples were isolated mould strains belonged to genera: Cladosporium sp., Fusarium sp., Penicillium sp. and yeasts genera as Kluyveromyces sp., Rhodotorula sp., Cryptococcus sp. The outcome of this study indicate mycotic contamination of goat milk that can pose serious health risk to consumers ...
The level of microbiological contamination with yeasts and moulds in samples of goat milk collected in spring, summer and autumn season was evaluated. Yeasts are present in a larger number in the analyzed goat milk with values between 1.9-3.42 log CFU/ml, contrast to molds that had a range of mean values between 1.47-3.02 log CFU/ml. Of the milk samples were isolated mould strains belonged to genera: Cladosporium sp., Fusarium sp., Penicillium sp. and yeasts genera as Kluyveromyces sp., Rhodotorula sp., Cryptococcus sp. The outcome of this study indicate mycotic contamination of goat milk that can pose serious health risk to consumers ...
Save 45% Solaray - Red Yeast Rice 600 mg 120 Capsules Red Yeast Rice 600 mg Non-Irradiated Citrinin-Free** Green Screened Solaray Brand Red Yeast Rice is rigorously tested to comply with our Green Screened process to assure the highest quality formula. The Green Screened process means our Red Yeast Rice is tested for impurities and contaminants, including citrinin (**screened using UPLC-MS down to 1ppm). This stringent screening allows us to offer a pure, high-quality, non-irradiated source of Red Yeast Rice. Vegetarian Capsules.
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Low prices on Red Yeast Rice! Red yeast rice contains rice that has been naturally fermented with red yeast. Its a unique natural product native to China that has been used in Asian traditional medical systems for centuries. It contains contains key compounds, similar to statins.
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1C0K: The x-ray structure of D-amino acid oxidase at very high resolution identifies the chemical mechanism of flavin-dependent substrate dehydrogenation.
1C0K: The x-ray structure of D-amino acid oxidase at very high resolution identifies the chemical mechanism of flavin-dependent substrate dehydrogenation.
Tellurium dioxide chemical properties, What are the chemical properties of Tellurium dioxide 7446-07-3, What are the physical properties of Tellurium dioxide ect.
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