The gene encoding a thermostable cellulase of family 12 was previously isolated from a Rhodothermus marinus through functional screening. CelA is a protein of 260 aminoacyl residues with a 28-residue amino-terminal signal peptide. Mature CelA was poorly synthesized in some Escherichia coli strains and not at all in others. Here we present an alternative approach for its heterologous production as a secreted polypeptide in Streptomyces. CelA was successfully over-expressed as a secreted polypeptide in Streptomyces lividans TK24. To this end, CelA was fused C-terminally to the secretory signal peptide of the subtilisin inhibitor protein (Sianidis et al. in J Biotechnol. 121: 498-507, 2006) from Streptomyces venezuelae and a new cloning strategy developed. Optimal growth media and conditions that stall biomass production promote excessive CelA secretion. Under optimal growth conditions in nutrient broth medium, significant amounts of mature CelA (50-90 mg/L or 100-120 mg/g of dry cell weight) are secreted

The pentose phosphate pathway is a process of glucose turnover that produces NADPH as reducing equivalents and pentoses as essential parts of nucleotides. There are two different phases in the pathway. One is irreversible oxidative phase in which glucose-6P is converted to ribulose-5P by oxidative decarboxylation, and NADPH is generated [MD:M00006]. The other is reversible non-oxidative phase in which phosphorylated sugars are interconverted to generate xylulose-5P, ribulose-5P, and ribose-5P [MD:M00007]. Phosphoribosyl pyrophosphate (PRPP) formed from ribose-5P [MD:M00005] is an activated compound used in the biosynthesis of histidine and purine/pyrimidine nucleotides. This pathway map also shows the Entner-Doudoroff pathway where 6-P-gluconate is dehydrated and then cleaved into pyruvate and glyceraldehyde-3P [MD:M00008 ...

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

article{569d6702-1354-4b3b-8b97-3c11c9807854, abstract = {Metabolic stress is a phenomenon often discussed in conjunction with recombinant protein production in Escherichia coli. This investigation shows how heterologous protein production, and the presence of host cell proteases is related to: i) IPTG induction, ii) cell mass concentration at the time of induction and iii) the presence of metabolites (glutamic acid or those from TSB) during the post induction phase of high-cell-density (HCD) fed-batch cultivations. Two thermostable xylanase variants and one thermostable cellulase, all originating from Rhodothermus marinus were expressed in E. coli strain BL21 (DE3). A three-fold difference in the specific activity of both xylanase variants [between 7000 and 21000 U / (g cdw)], was observed under the different conditions tested. Upon induction at high cell-mass concentrations employing a nutrient feed devoid of the metabolites above, the specific activity of the xylanase variants, was initially ...

In the present study, TreZ from Zunongwangia sp. was isolated and expressed in E. coli BL21 (DE3). The optimum temperature for TreZ is 50 °C, which is higher than that of many previously reported trehalases, such as those in Apis mellifera L 7 °C [22], Rhodotorula rubra 30 °C [23], Saccharomyces cerevisiae 40 °C [24], and Rhizopus microsporus var. rhizopodiformis 45 °C [25]. However, a trehalase from a thermophilic bacterium Rhodothermus marinus has a higher optimal temperature of 88 °C than TreZ [26].. Another remarkable characteristic of TreZ is its extreme salt-tolerance (Fig. 3). The enzyme was found to be active over a wide NaCl concentration range (0-5 M exhibiting the maximum activity at 1 M NaCl (136 %) and retaining more than 100 % of its original activity at 0.5-3.5 M NaCl. This behavior is similar to that of a xylanase and two amylases from Zunongwangia sp., which showed the optimum activity at 3, 1.5 and 2 M NaCl, respectively [27-29]. TreZ retains activity with or without NaCl ...

Quiescence (G0) represents an assortment of reversible, cell cycle-arrested states that are resistant to unfavorable conditions and associated with cancer persistence. G0 involves regulated gene expression with selective mRNA expression and decreased canonical translation. Low mTOR activity in G0 activates the cap complex inhibitor, eIF4EBP, and impairs canonical translation. The alternative translation mechanisms in G0 remain to be uncovered. Our data show that microRNAs, regulatory, non-coding RNAs that target distinct mRNAs to alter gene expression, can associate with alternative complexes and translation factors to regulate specific mRNA translation in G0. One subset of transcripts expressed in G0 includes specific mRNAs recruited by an FXR1a-associated microRNP (microRNA-protein complex) for translation activation in G0 mammalian cells. MicroRNPs predominantly mediate repression and downregulation; however, FXR1a-microRNP lacks conventional microRNP repressors, and instead, contains a ...

Although ovarian cancer implants can be targeted using an avidin-3 rhodamineX complex, there are several reasons to consider alternative complexes. GmSA-20ROX is substantially less immunogenic compared with avidin, and GmSA has been used as the basis for a technetium-99m-labeled radiopharmaceutical to assess hepatic reserve in humans. Additionally, GmSA-20ROX binds target cells more rapidly and more efficiently than avidin due to its multivalency (7, 16). Because GmSA synthesis involves the conjugation of 23 d-galactosamines reacted with carboxyl groups rather than amino groups on an albumin molecule (7), there are multiple binding sites for the d-galactose receptor and the molecule has a favorably high isoelectric point (7, 16). Moreover, activation via dequenching of the rhodamineX after cellular internalization provides a generalizable platform for targeted fluorescent probes by exchanging the targeting moiety (5). We used the d-galactose receptor as a target in this study because it is ...

These proteins are members of the NhaD Na+:H+ Antiporter (NhaD) Family (TC 2.A.62). A single member of the NhaD family has been characterized. This protein is the NhaD protein of Vibrio parahaemolyticus which has 12 GES predicted transmembrane regions. It has been shown to catalyze Na+/H+ antiport, but Li+ can also be a substrate ...

I dont mean to bash AC or anything (or offend anyone somehow) but Im probably going to skip as this franchise IMO is milked. I dont know how they can make the gameplay any different from the last one and how is hunting possibly going to make a difference? In economy? I wanted ACIII to be all about Desmond which hes more than likely going to get a few missions. The way I feel about ACIII is the same about how I felt with AC:R which is nothing new. I thought AC:R would have at least more than a few missions with Altair but all I got was what? 5?? And I know this might sound dumb to some people but why cant they for once make one full game about Desmond since he is the main character, not his ancestors. I dont know how to explain it but Im done with AC. AC died when AC:B came out.. ...

Marinus Pharmaceuticals is a clinical stage biopharmaceutical company dedicated to the development of ganaxolone, offering a new mechanism of action with demonstrated safety and convenient dosing for patients affected by epilepsy and neuropsychiatric disorders.

Hi guys, so after a long time of thinking, ordering, waiting, re-designing and putting together parts I am finally finalizing my MendelMax 1.5 Here are some pictures, the machine is still not done, but there is already a lot of work done. This is the second 3D-printer Im building, after working wi

Wood cell walls represent highly complex biocomposites that consists of repertoires of polysaccharides and lignins integrated into a three-dimensional conglomerate [1, 2]. At the microstructural level, wood cell walls are composed of both primary- and dominating secondary cell wall layers in which the major (cellulose, lignin and hemicelluloses) and minor (pectins, proteins) chemical components are heterogeneously dispersed and integrated. Understanding the cell walls complexity is of great interest both for plant biology and for plant processing in food and pulp industry.. One of the key main challenges for an improved understanding of wood cell wall structure especially, in relation to properties, is an ability to specifically localise and visualised the spatial distribution of the major polysaccharide components (cellulose and hemicelluloses) in vivo. In lignified cell walls this is quite challenging since the hemicelluloses interface between the cellulose and lignin forming a heterogeneous ...

This paper deals with mechanical fault monitoring in induction machines from stator current measurements. The considered faults lead to amplitude and/or phase modulations of the measured stator current. The different faults can be characterized by their time-frequency signatures via the Wigner distribution. The time-frequency representations apply to complex signals that may be obtained through the Hilbert transform of the real measured signal. In case of high frequency modulations, it can not be taken advantage of the time frequency signatures. This study proposes an alternative complex signal representation for modulated stator currents. The so-called space vector is obtained through the Concordia transform. From three stator current measurements, the Concordia transform builds a complex vector which conveniently carries the information about phase and amplitude modulations. This paper applies and compares the Wigner distribution computed with the Hilbert and Concordia transforms in case of simulated

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Talk , contribs)‎ . . (2,996 bytes) (+2,996)‎ . . (Created page with ,!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption --, {{UnderConstruct...) ...

Today was Pauls last day of treatment. It was a gorgeous sunny day so after he wrapped up, we walked into town. Paul went to the gym, I took a walk and then we celebrated with CAKE. Tomorrow morning at 5:45 AM local time we head home via a very long travel day that takes…

Beli Jual LIBBY 3 Pcs Popok Tali Bayi Baby Motif 0-3M IX-86G Barang Murah dari Marinus marinus974 - Jakarta Selatan hanya di Bukalapak

Catalyzes the hydrolysis of glucosylglycerate (GG) to glycerate and glucose (PubMed:25341489, PubMed:31316802). Involved in recovery from nitrogen starvation by promoting the rapid mobilization of the glucosylglycerate that accumulates under these conditions (PubMed:25341489). Can also hydrolyze mannosylglycerate (MG), with tenfold lower efficiency (PubMed:31316802).

ltsikora 617 tiscali cz djamale csc 470 ofir dk carlos olea reyes 167 ybb ne jp gerry fleming33 191 bigmir net stephanie marinus 284 mercadolibre ar p c kroon 518 akeonet com ...

On Mars, significant amounts (0.4 - 0.6 %) of perchlorate ions were detected in dry soil by the Phoenix Wet Chemistry Laboratory and later confirmed with the Mars Science Laboratory. Therefore, the ability of Hydrogenothermus marinus, a desiccation tolerant bacterium, to survive and grow in the presence of perchlorates was determined. Results indicated that H. marinus was able to tolerate concentrations of sodium perchlorate up to 200 mM (≙ 1.6 %) during cultivation without any changes in its growth pattern. After the addition of up to 440 mM (≙ 3.7 %) sodium perchlorate, H. marinus showed significant changes in cell morphology; from single motile short rods to long cell chains up to 80 cells. Furthermore, it was shown that the known desiccation tolerance of H. marinus is highly influenced by a pre-treatment with different perchlorates; additive effects of desiccation and perchlorate treatments are visible in a reduced survival rate. These data demonstrate that thermophiles, especially H. marinus,

Note (January 2018): We are now in full development of our individual CBM pages in CAZypedia and our long-term goal is to have complete coverage of all of the CBM families represented in the CAZy database. This effort was initiated by Alicia Lammerts van Bueren, together with the Senior Curators, and is now being led by Elizabeth Ficko-Blean. If you would like to become involved in the development of the CBM pages, please contact Elizabeth Ficko-Blean at ...

InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.

From BioPortfolio: SummaryMarinus Pharmaceuticals Inc Marinus is a biopharmaceutical company that manufactures and commercializes therapeutics for the treatment of epilepsy and ot...

Staphylothermus marinus is a marine organism that was isolated from hydrothermal sediment off the the coast of Vulcano Island in Italy. It can also be found from black smokers on the ocean floor. In a rich medium, Staphylothermus marinus grows in an optimum temperature of 92 degrees Celsius, but when nutrients are sparce, the optimum temperature drops to 85 degrees Celsius. For growth in a lab, a complex nutrient source is needed for optimum growth. The morphology of the Staphylothermus marinus can differ depending on the nutrients available. When nutrients are plentiful, Staphylothermus marinus grow as giant cells in a slightly irregular coccus shape with diameters up to 15 mm. Low nutrient concentrations produce little cells with diameters ranging from 0.5 to 1.0 mm. Up to 100 of these cells can cluster together to form grape-like groups. S. marinus is related to Aeropyrum pernix, Hyperthermus butylicus, and Ignicoccus hospitalis. (5) Describe the appearance, habitat, etc. of the organism, ...

1. One of the major steps in protein folding is getting rid of water from the protein core. In S. marinus, large water clusters, 7 to 9 water molecules, have been found in the nonpolar cavities of the tetrabrachion. At about 110 degrees Celsius, the clustering becomes unfavorable and signals the drying process to begin. This drying process is the start of protein denaturation. The study shows that the unusually stable tetrabrachion structure can possibly be explained by the large hydrophobic cavities that are the binding sites for two proteases. (4) 2. One of the proteases in S. marinus has been tested to be one of the most stable proteases known to man. It is part of the subtilisin family and is the only known ezyme that is part of the S-layer. It has a very high resistance toward denaturing agents. (7) 3. The phosphoenolpyruvate synthase of S. marinus has been an interest to academia due to its unusual stability in high temperatures. The synthase forms a homomultimeric complex which is 83 kDa ...

Marinus Pharmaceuticals, Inc. (Nasdaq: MRNS), a pharmaceutical company dedicated to the development of innovative therapeutics to treat rare seizure disorders,

Stat SI TUESDAY, DECEMBER 20,183a . . - -1) fc.AAl WHOLE i0. 14 JO ritlNTBD AND IMJCMSHKD BY SCOTT & WRIGHT. Office on Stale-street, third door went of the Clinton Bank, j. n vilh aciii:, eimtor. Qj The Jot.R KAti Id puhliidied once n work, cenorully, mid twice a week diiriinr, the nesaion sf tliu Htute Lcuinliiture. 1ricc, Tliree Do I! sirs a year in ndvttmC, or Threo liolliirs and Kilty Cents at the end of the yenr. No sul Kcrilier allowed to discontinue while lie remains Indehted to the oltice. I.elturs on hiLiinvM should he udtlresned to t lie Iuhlwlicrij. COMMUNICATIONS. For the Ohio State Juumul, Nr. Editor: I proceed to fulfill my promise, to furnish you with a plan for tlio, rational umusutnent of tlio youth of your city, in such n way us to culiivate their mituls nn.l thur inor.ila, anil improve thuir taste. without the dumrcr of leading them in tlio road to ruin. I propose that vour citizenn call n public mretins. LV advortisemctit in the principal papers) to take ill in Tintters ...

Stat SI TUESDAY, DECEMBER 20,183a . . - -1) fc.AAl WHOLE i0. 14 JO ritlNTBD AND IMJCMSHKD BY SCOTT & WRIGHT. Office on Stale-street, third door went of the Clinton Bank, j. n vilh aciii:, eimtor. Qj The Jot.R KAti Id puhliidied once n work, cenorully, mid twice a week diiriinr, the nesaion sf tliu Htute Lcuinliiture. 1ricc, Tliree Do I! sirs a year in ndvttmC, or Threo liolliirs and Kilty Cents at the end of the yenr. No sul Kcrilier allowed to discontinue while lie remains Indehted to the oltice. I.elturs on hiLiinvM should he udtlresned to t lie Iuhlwlicrij. COMMUNICATIONS. For the Ohio State Juumul, Nr. Editor: I proceed to fulfill my promise, to furnish you with a plan for tlio, rational umusutnent of tlio youth of your city, in such n way us to culiivate their mituls nn.l thur inor.ila, anil improve thuir taste. without the dumrcr of leading them in tlio road to ruin. I propose that vour citizenn call n public mretins. LV advortisemctit in the principal papers) to take ill in Tintters ...

Oprah is obsessed with the Bartesian Cocktail Machine and so are we. We tried the Keurig-like cocktail maker - check out our full review.

Information Published by: Centers for Disease Control and Prevention Español , Other Languages Flu vs. COVID-19 Influenza (Flu) and COVID-19 are both contagious respiratory illnesses, but they are caused by different viruses. COVID-19 is caused by infection with a new coronavirus (called SARS-CoV-2) and flu is caused by infection with influenza viruses. Because some of the symptoms of flu and COVID-19 are similar, …. ...